Rh5CG588200

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010441
Physical Location & Seq
Reverse (-)
83335110 .. 83338830
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG588200.1

Sequence Viewer

Length: 747 bp
ATGGAGGGAAGCTCGCTTCTCAGTCTCAGCTGCTCCTCTGCTCTTCCCAATTTAATACCCACTCCTTCTTACTCCTCTCTAAGCTTTACTCTCTCCCACTCCTCATCCTCTTCTTCTTCTTCTTCTCCTTTGCTATTTGGTAGATCCCATCTCGTGGGTTTAATCCCAAGACCATTCACTGTTCGAACCTCTCTCCGCACTTCCACGTTCGCTGCCGTCGATGAAGCTCTCGAAAAGTCCAACTCTGATTCCAGAGATATGTTGCCCAAGATTGACAAGAGTGGCAGATTTTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTCTCCATTATTTATGCTTCCTGCTTAGAAGGCTCCGACCCGGTACGCTTATTTGAAAAGCGAATGAATGTCCGTCGAGAACCCGGGTATGAATTTGACAAGGCTTCATTGTTGGAATATAATCCCATGAGCTTCGGAGGACCCCCTGTCACTGTTCAAACTGTTGAAGAAGCAGATGAGCTCCTGCTCAATGACCAAAAGGAATCTGCAATCGAAGCGGAAGTTCTTGCAGCTCCTCCAAAGTTGGTATACAGCAAACTGATTTTAAGATTTGCAAGGAAACTTTTGGTTGCAGTTATGGATAAATGGGACAGTCATGTGCTTGTCATTGACAGAGTTGCCCCTCCAAATTGGAAGGTGTGTACAAGGTTTAGAGGTCAAGTCACATTTTTACACTTCCACATTTGTCTTTTGTTCCAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

248

Amino Acids

27.5

Weight (kDa)

8.21

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 154
AccI GTMKAC 1 cut(s) 570
AciI CCGC 3 cut(s) 196, 306, 539
AclWI GGATC 1 cut(s) 138
AcsI RAATTY 1 cut(s) 413
AfaI GTAC 2 cut(s) 366, 685
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 3 cut(s) 377, 479, 488
AhdI GACNNNNNGTC 1 cut(s) 467
AluBI AGCT 8 cut(s) 12, 30, 84, 227, 316, 453, 502, 554
AluI AGCT 8 cut(s) 12, 30, 84, 227, 316, 453, 502, 554
Alw21I GWGCWC 2 cut(s) 318, 504
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 404
ApeKI GCWGC 4 cut(s) 30, 212, 294, 551
ApoI RAATTY 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 3 cut(s) 362, 405, 406
AsuII TTCGAA 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 461
BanII GRGCYC 2 cut(s) 318, 504
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 318, 504
BbvI GCAGC 4 cut(s) 17, 199, 306, 563
BccI CCATC 1 cut(s) 156
BceAI ACGGC 1 cut(s) 200
BcnI CCSGG 3 cut(s) 362, 405, 406
BcoDI GTCTC 1 cut(s) 29
BisI GCNGC 5 cut(s) 31, 213, 295, 306, 552
BlsI GCNGC 5 cut(s) 32, 214, 296, 307, 553
Bme1390I CCNGG 3 cut(s) 362, 405, 406
Bme18I GGWCC 1 cut(s) 461
BmeRI GACNNNNNGTC 1 cut(s) 467
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 2 cut(s) 355, 463
BmrFI CCNGG 3 cut(s) 362, 405, 406
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 184
BpuMI CCSGG 3 cut(s) 362, 405, 406
BsaJI CCNNGG 1 cut(s) 404
Bsc4I CCNNNNNNNGG 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 2 cut(s) 34, 40
BseRI GAGGAG 4 cut(s) 25, 64, 91, 546
BseXI GCAGC 4 cut(s) 17, 199, 306, 563
BsiHKAI GWGCWC 2 cut(s) 318, 504
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 2 cut(s) 362, 405
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 644
BsoBI CYCGRG 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 318, 504
Bsp1407I TGTACA 1 cut(s) 683
Bsp143I GATC 1 cut(s) 143
BspACI CCGC 3 cut(s) 196, 306, 539
BspCNI CTCAG 2 cut(s) 33, 39
BspLI GGNNCC 2 cut(s) 355, 463
BspPI GGATC 1 cut(s) 138
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 683
BssECI CCNNGG 1 cut(s) 404
BssMI GATC 1 cut(s) 143
BssNAI GTATAC 1 cut(s) 571
BssSI CACGAG 1 cut(s) 152
Bst1107I GTATAC 1 cut(s) 571
Bst2BI CACGAG 1 cut(s) 152
Bst4CI ACNGT 4 cut(s) 181, 475, 484, 635
Bst6I CTCTTC 2 cut(s) 48, 115
BstAPI GCANNNNNTGC 1 cut(s) 291
BstAUI TGTACA 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 14, 318
BstDEI CTNAG 4 cut(s) 20, 26, 80, 346
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 1 cut(s) 146
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 3 cut(s) 291, 351, 536
BstSCI CCNGG 3 cut(s) 360, 403, 404
BstV1I GCAGC 4 cut(s) 17, 199, 306, 563
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BstZ17I GTATAC 1 cut(s) 571
BtsCI GGATG 1 cut(s) 104
BtsIMutI CAGTG 2 cut(s) 177, 471
Cac8I GCNNGC 2 cut(s) 14, 318
Cfr13I GGNCC 1 cut(s) 461
Cfr9I CCCGGG 1 cut(s) 404
Csp6I GTAC 2 cut(s) 365, 684
CviAII CATG 2 cut(s) 448, 638
CviQI GTAC 2 cut(s) 365, 684
DdeI CTNAG 4 cut(s) 20, 26, 80, 346
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
DriI GACNNNNNGTC 1 cut(s) 467
Eam1104I CTCTTC 2 cut(s) 48, 115
Eam1105I GACNNNNNGTC 1 cut(s) 467
EarI CTCTTC 2 cut(s) 48, 115
Ecl136II GAGCTC 2 cut(s) 316, 502
Eco24I GRGCYC 2 cut(s) 318, 504
Eco47I GGWCC 1 cut(s) 461
Eco53kI GAGCTC 2 cut(s) 316, 502
Eco88I CYCGRG 1 cut(s) 404
EcoICRI GAGCTC 2 cut(s) 316, 502
EcoO109I RGGNCCY 1 cut(s) 461
EcoT38I GRGCYC 2 cut(s) 318, 504
FaeI CATG 2 cut(s) 451, 641
FaiI YATR 8 cut(s) 260, 336, 411, 441, 449, 571, 620, 639
FaqI GGGAC 1 cut(s) 644
FatI CATG 2 cut(s) 447, 637
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 31, 213, 295, 306, 552
FokI GGATG 1 cut(s) 91
FriOI GRGCYC 2 cut(s) 318, 504
Fsp4HI GCNGC 5 cut(s) 31, 213, 295, 306, 552
GluI GCNGC 5 cut(s) 31, 213, 295, 306, 552
HapII CCGG 2 cut(s) 362, 405
Hin1II CATG 2 cut(s) 451, 641
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 2 cut(s) 248, 524
HpaII CCGG 2 cut(s) 362, 405
Hpy166II GTNNAC 2 cut(s) 571, 684
Hpy188I TCNGA 3 cut(s) 247, 358, 458
Hpy188III TCNNGA 3 cut(s) 230, 252, 398
Hpy8I GTNNAC 2 cut(s) 571, 684
Hpy99I CGWCG 2 cut(s) 221, 399
HpyAV CCTTC 3 cut(s) 75, 344, 670
HpyCH4III ACNGT 4 cut(s) 181, 475, 484, 635
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 5 cut(s) 294, 530, 551, 596, 614
HpyF10VI GCNNNNNNNGC 3 cut(s) 291, 351, 536
HpyF3I CTNAG 4 cut(s) 20, 26, 80, 346
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 2 cut(s) 451, 641
Kzo9I GATC 1 cut(s) 143
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 4 cut(s) 38, 359, 507, 559
LpnPI CCDG 8 cut(s) 265, 313, 322, 355, 375, 418, 480, 518
Lsp1109I GCAGC 4 cut(s) 17, 199, 306, 563
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 469, 703
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MboII GAAGA 7 cut(s) 35, 102, 105, 108, 111, 114, 500
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 318, 504
MluCI AATT 3 cut(s) 49, 413, 670
MmeI TCCRAC 3 cut(s) 264, 381, 414
MnlI CCTC 9 cut(s) 46, 85, 112, 118, 199, 452, 567, 675, 689
MseI TTAA 3 cut(s) 53, 161, 587
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 2 cut(s) 362, 405
MspR9I CCNGG 3 cut(s) 362, 405, 406
MwoI GCNNNNNNNGC 3 cut(s) 291, 351, 536
NciI CCSGG 3 cut(s) 362, 405, 406
NdeII GATC 1 cut(s) 143
NlaIII CATG 2 cut(s) 451, 641
NlaIV GGNNCC 2 cut(s) 355, 463
NmuCI GTSAC 2 cut(s) 469, 703
NspV TTCGAA 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 2 cut(s) 248, 524
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 5 cut(s) 32, 214, 296, 307, 553
PpuMI RGGWCCY 1 cut(s) 461
Psp124BI GAGCTC 2 cut(s) 318, 504
Psp5II RGGWCCY 1 cut(s) 461
PspN4I GGNNCC 2 cut(s) 355, 463
PspPI GGNCC 1 cut(s) 461
PspPPI RGGWCCY 1 cut(s) 461
PsuI RGATCY 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 2 cut(s) 366, 685
RsaNI GTAC 2 cut(s) 365, 684
SacI GAGCTC 2 cut(s) 318, 504
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 3 cut(s) 53, 161, 587
SatI GCNGC 5 cut(s) 31, 213, 295, 306, 552
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 3 cut(s) 362, 405, 406
SduI GDGCHC 2 cut(s) 318, 504
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 1 cut(s) 461
SmaI CCCGGG 1 cut(s) 406
Sse9I AATT 3 cut(s) 49, 413, 670
SsiI CCGC 3 cut(s) 196, 306, 539
SstI GAGCTC 2 cut(s) 318, 504
StyD4I CCNGG 3 cut(s) 360, 403, 404
TaaI ACNGT 4 cut(s) 181, 475, 484, 635
TaiI ACGT 1 cut(s) 209
TaqI TCGA 5 cut(s) 184, 219, 231, 397, 534
TasI AATT 3 cut(s) 49, 413, 670
TatI WGTACW 1 cut(s) 683
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 2 cut(s) 248, 524
Tru1I TTAA 3 cut(s) 53, 161, 587
Tru9I TTAA 3 cut(s) 53, 161, 587
TscAI CASTG 2 cut(s) 184, 478
TseFI GTSAC 2 cut(s) 469, 703
TseI GCWGC 4 cut(s) 30, 212, 294, 551
Tsp45I GTSAC 2 cut(s) 469, 703
TspDTI ATGAA 4 cut(s) 237, 401, 417, 426
TspGWI ACGGA 1 cut(s) 383
TspMI CCCGGG 1 cut(s) 404
TspRI CASTG 2 cut(s) 184, 478
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 1 cut(s) 413
XmaI CCCGGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.