Rh5DG574500

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010441
Physical Location & Seq
Reverse (-)
88160660 .. 88163507
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG574500.1

Sequence Viewer

Length: 897 bp
ATGGAGGGAAGCTCGCTTCTCAGTCTCAGCTGCTCCTCTGCTCTTCCCAGTTTGATACCCACTCCCTCTTACTCCTCTATAAGCTTCAACTCCCACTCCTCGTCTTCTTCTTCTTTGCTATTCGGAAAATCATATCTCGTGGGTTTAATCCCAAGACCATTCACCGTTCGAACCTCTCTCCGCACTTCCACGTTCGCCGCCGTCGATGAAGCTCTCGAAAAGCCCAACTCTGATTCCAGAGATATGTTGCCCAAGATTGACAAGAGTGGCAGATTTTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTCTCCATTATTTATGCTTCCTGCTTAGAAGGTTCCGACCCAGTAAGGTTATTTGAAAAGCGAATGAATGTCCGTCGAGAACCCGGGTATGAATTTGACAAGGCTTCATTGTTGGAATATAATCCCATGAGCTTTGGAGGACCCCCTGTCACTGTTCAAACTGTTGAAGAAGCAGATGAGCTCCTGCTCAATGACCAAAAGGAATCTGCAATCGAAGCGGAAGTTCTTGCAGCTCCTCCAAAGTTGGTATACAGCAAACTGATTTTGAGATTTGCAAGGAAACTTTTGGTTGCAGTTATGGATAAATGGGACAGTCATGTGCTTGTCATTGACAGAGTTGCCCCTCCAAATTGGAAGGATGAGCCAGGAAGCAGAATCTTGGAGCTTTGCATCCTTCACTTGGCAATGTCAGAAATAACAGTGTTAGGAACGCGACACCAGATTGTCATTAATGAGGCTGTAGATCTTGCTAAACGTTTCTGTGATGGGTCAGCCCCCCGCACAATCAATGGTTGTCTTAGAACATTCGTCAATGGCCTTGAAGAATCTGGTTTAACTCCAGCTTTGGGGAAACAGAAAACCGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

298

Amino Acids

32.67

Weight (kDa)

6.46

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusB PF01029 191 - 279 4.2e-08 NusB family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 555
AccII CGCG 1 cut(s) 739
AciI CCGC 5 cut(s) 181, 198, 291, 524, 805
AclI AACGTT 1 cut(s) 781
AcsI RAATTY 1 cut(s) 398
AfiI CCNNNNNNNGG 2 cut(s) 706, 872
AgsI TTSAA 5 cut(s) 88, 362, 464, 473, 848
AhdI GACNNNNNGTC 1 cut(s) 452
AjnI CCWGG 1 cut(s) 670
Alw21I GWGCWC 2 cut(s) 303, 489
Alw26I GTCTC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 389
AoxI GGCC 1 cut(s) 841
ApeKI GCWGC 3 cut(s) 30, 279, 536
ApoI RAATTY 1 cut(s) 398
AseI ATTAAT 1 cut(s) 756
AspS9I GGNCC 1 cut(s) 446
AsuC2I CCSGG 2 cut(s) 390, 391
AsuHPI GGTGA 1 cut(s) 154
AsuII TTCGAA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 389
AvaII GGWCC 1 cut(s) 446
BanII GRGCYC 2 cut(s) 303, 489
BauI CACGAG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 303, 489
BbvI GCAGC 3 cut(s) 17, 291, 548
BccI CCATC 1 cut(s) 785
BceAI ACGGC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 672
BcnI CCSGG 2 cut(s) 390, 391
BcoDI GTCTC 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 765
BglII AGATCT 1 cut(s) 769
BisI GCNGC 5 cut(s) 31, 198, 280, 291, 537
BlsI GCNGC 5 cut(s) 32, 199, 281, 292, 538
Bme1390I CCNGG 3 cut(s) 390, 391, 672
Bme18I GGWCC 1 cut(s) 446
BmeRI GACNNNNNGTC 1 cut(s) 452
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 2 cut(s) 340, 448
BmrFI CCNGG 3 cut(s) 390, 391, 672
BmrI ACTGGG 2 cut(s) 42, 341
BmsI GCATC 1 cut(s) 705
BmuI ACTGGG 2 cut(s) 42, 341
BpiI GAAGAC 1 cut(s) 96
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 849
Bpu14I TTCGAA 1 cut(s) 169
BpuMI CCSGG 2 cut(s) 390, 391
BsaJI CCNNGG 1 cut(s) 389
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 2 cut(s) 706, 872
Bse1I ACTGG 2 cut(s) 48, 347
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 389
BseGI GGATG 2 cut(s) 670, 696
BseLI CCNNNNNNNGG 2 cut(s) 706, 872
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 34, 40
BseNI ACTGG 2 cut(s) 48, 347
BseRI GAGGAG 4 cut(s) 25, 64, 88, 531
BseXI GCAGC 3 cut(s) 17, 291, 548
Bsh1236I CGCG 1 cut(s) 739
BshFI GGCC 1 cut(s) 843
BsiHKAI GWGCWC 2 cut(s) 303, 489
BsiHKCI CYCGRG 1 cut(s) 389
BsiSI CCGG 1 cut(s) 390
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 2 cut(s) 706, 872
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 1 cut(s) 843
BsoBI CYCGRG 1 cut(s) 389
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 303, 489
Bsp143I GATC 1 cut(s) 769
BspACI CCGC 5 cut(s) 181, 198, 291, 524, 805
BspANI GGCC 1 cut(s) 843
BspCNI CTCAG 2 cut(s) 33, 39
BspFNI CGCG 1 cut(s) 739
BspLI GGNNCC 2 cut(s) 340, 448
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 2 cut(s) 48, 347
BssECI CCNNGG 1 cut(s) 389
BssMI GATC 1 cut(s) 769
BssNAI GTATAC 1 cut(s) 556
BssSI CACGAG 1 cut(s) 137
Bst1107I GTATAC 1 cut(s) 556
Bst2BI CACGAG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 6 cut(s) 166, 460, 469, 620, 727, 889
Bst6I CTCTTC 1 cut(s) 48
BstAPI GCANNNNNTGC 1 cut(s) 276
BstBI TTCGAA 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 14, 303
BstDEI CTNAG 4 cut(s) 20, 26, 331, 824
BstF5I GGATG 2 cut(s) 670, 696
BstFNI CGCG 1 cut(s) 739
BstKTI GATC 1 cut(s) 772
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 1 cut(s) 769
BstMWI GCNNNNNNNGC 2 cut(s) 276, 521
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 3 cut(s) 388, 389, 670
BstSFI CTRYAG 1 cut(s) 765
BstUI CGCG 1 cut(s) 739
BstV1I GCAGC 3 cut(s) 17, 291, 548
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BstZ17I GTATAC 1 cut(s) 556
BsuRI GGCC 1 cut(s) 843
BtsCI GGATG 2 cut(s) 670, 696
BtsIMutI CAGTG 2 cut(s) 456, 732
Cac8I GCNNGC 2 cut(s) 14, 303
Cfr13I GGNCC 1 cut(s) 446
Cfr9I CCCGGG 1 cut(s) 389
CviAII CATG 2 cut(s) 433, 623
DdeI CTNAG 4 cut(s) 20, 26, 331, 824
DpnI GATC 1 cut(s) 771
DpnII GATC 1 cut(s) 769
DriI GACNNNNNGTC 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 48
Eam1105I GACNNNNNGTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 48
Ecl136II GAGCTC 2 cut(s) 301, 487
Eco24I GRGCYC 2 cut(s) 303, 489
Eco47I GGWCC 1 cut(s) 446
Eco53kI GAGCTC 2 cut(s) 301, 487
Eco88I CYCGRG 1 cut(s) 389
EcoICRI GAGCTC 2 cut(s) 301, 487
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 670
EcoT38I GRGCYC 2 cut(s) 303, 489
FaeI CATG 2 cut(s) 436, 626
FaqI GGGAC 1 cut(s) 629
FatI CATG 2 cut(s) 432, 622
FauI CCCGC 1 cut(s) 812
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 5 cut(s) 31, 198, 280, 291, 537
FokI GGATG 2 cut(s) 677, 683
FriOI GRGCYC 2 cut(s) 303, 489
Fsp4HI GCNGC 5 cut(s) 31, 198, 280, 291, 537
GluI GCNGC 5 cut(s) 31, 198, 280, 291, 537
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 1 cut(s) 843
HapII CCGG 1 cut(s) 390
Hin1II CATG 2 cut(s) 436, 626
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 4 cut(s) 233, 509, 681, 851
HpaII CCGG 1 cut(s) 390
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 4 cut(s) 125, 232, 343, 718
Hpy188III TCNNGA 3 cut(s) 215, 237, 383
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 2 cut(s) 206, 384
HpyAV CCTTC 3 cut(s) 329, 655, 710
HpyCH4III ACNGT 6 cut(s) 166, 460, 469, 620, 727, 889
HpyCH4IV ACGT 2 cut(s) 191, 781
HpyCH4V TGCA 6 cut(s) 279, 515, 536, 581, 599, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 276, 521
HpyF3I CTNAG 4 cut(s) 20, 26, 331, 824
HpySE526I ACGT 2 cut(s) 191, 781
Hsp92II CATG 2 cut(s) 436, 626
Kzo9I GATC 1 cut(s) 769
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 4 cut(s) 38, 492, 544, 688
Lsp1109I GCAGC 3 cut(s) 17, 291, 548
LweI GCATC 1 cut(s) 705
MaeII ACGT 2 cut(s) 191, 781
MaeIII GTNAC 2 cut(s) 454, 889
MalI GATC 1 cut(s) 771
MboI GATC 1 cut(s) 769
MboII GAAGA 6 cut(s) 35, 96, 99, 102, 485, 860
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 2 cut(s) 303, 489
MluCI AATT 2 cut(s) 398, 655
MmeI TCCRAC 2 cut(s) 366, 399
MnlI CCTC 9 cut(s) 46, 76, 85, 109, 184, 437, 552, 660, 754
MseI TTAA 3 cut(s) 146, 756, 860
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 3 cut(s) 390, 391, 672
MvaI CCWGG 1 cut(s) 672
MvnI CGCG 1 cut(s) 739
MwoI GCNNNNNNNGC 2 cut(s) 276, 521
NciI CCSGG 2 cut(s) 390, 391
NdeII GATC 1 cut(s) 769
NlaIII CATG 2 cut(s) 436, 626
NlaIV GGNNCC 2 cut(s) 340, 448
NmuCI GTSAC 2 cut(s) 454, 889
NspV TTCGAA 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 4 cut(s) 233, 509, 681, 851
PkrI GCNGC 5 cut(s) 32, 199, 281, 292, 538
PpuMI RGGWCCY 1 cut(s) 446
PshBI ATTAAT 1 cut(s) 756
Psp124BI GAGCTC 2 cut(s) 303, 489
Psp1406I AACGTT 1 cut(s) 781
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 2 cut(s) 340, 448
PspPI GGNCC 1 cut(s) 446
PspPPI RGGWCCY 1 cut(s) 446
PsuI RGATCY 1 cut(s) 769
PvuII CAGCTG 1 cut(s) 30
SacI GAGCTC 2 cut(s) 303, 489
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 3 cut(s) 146, 756, 860
SatI GCNGC 5 cut(s) 31, 198, 280, 291, 537
Sau3AI GATC 1 cut(s) 769
Sau96I GGNCC 1 cut(s) 446
ScrFI CCNGG 3 cut(s) 390, 391, 672
SduI GDGCHC 2 cut(s) 303, 489
SfaNI GCATC 1 cut(s) 705
SfcI CTRYAG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 169
SinI GGWCC 1 cut(s) 446
SmaI CCCGGG 1 cut(s) 391
Sse9I AATT 2 cut(s) 398, 655
SsiI CCGC 5 cut(s) 181, 198, 291, 524, 805
SstI GAGCTC 2 cut(s) 303, 489
StyD4I CCNGG 3 cut(s) 388, 389, 670
TaaI ACNGT 6 cut(s) 166, 460, 469, 620, 727, 889
TaiI ACGT 2 cut(s) 194, 784
TaqI TCGA 5 cut(s) 169, 204, 216, 382, 519
TasI AATT 2 cut(s) 398, 655
TauI GCSGC 2 cut(s) 200, 293
TfiI GAWTC 4 cut(s) 233, 509, 681, 851
Tru1I TTAA 3 cut(s) 146, 756, 860
Tru9I TTAA 3 cut(s) 146, 756, 860
TscAI CASTG 2 cut(s) 463, 732
TseFI GTSAC 2 cut(s) 454, 889
TseI GCWGC 3 cut(s) 30, 279, 536
Tsp45I GTSAC 2 cut(s) 454, 889
TspDTI ATGAA 4 cut(s) 222, 386, 402, 411
TspGWI ACGGA 1 cut(s) 368
TspMI CCCGGG 1 cut(s) 389
TspRI CASTG 2 cut(s) 463, 732
VpaK11BI GGWCC 1 cut(s) 446
VspI ATTAAT 1 cut(s) 756
XapI RAATTY 1 cut(s) 398
XmaI CCCGGG 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.