Rmu_sc0001137.1_g000001

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001137.1
OG0010441
Physical Location & Seq
Forward (+)
6650 .. 9848
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001137.1_g000001.1.cds

Sequence Viewer

Length: 924 bp
atggagggaagctcgcttctcagtctcagctgctcctctgctcttcccaatttaatacccactccttcttactcctctctaagctttactctctcccactcctcatcctcttcttcttcttcttctcctttgctatttggtagatcccatctcgtgggtttaatcccaagaccattcactgttcgaacctctctccgcacttccacgttcgctgccgtcgatgaagctctcgaaaagtccaactctgattccagagatatgttgcccaagattgacaagagtggcagattttgcagccccagagccgccagagagctcgctctctccattatttatgcttcctgcttagaaggctccgacccagtacgcttatttgaaaagcgaatgaatgtccgtcgagaacccgggtatgaatttgacaaggcttcattgttggaatataatcccatgagcttcggaggaccccctgtcacagttcaaactgttgaagaagcagatgagctcctgctcaatgacgaaaaggaatctgcaatcgaagcggaagttctagcagctcctccaaagttggtatacagcaaactgattttaagatttgcaaggaaacttttggttgcagttatggataaatgggacagtcatgtgcttgtcattgacagagttgcccctccaaattggaaggatgagccaggaagcagaatcttggagctttgcatccttcacttggcaatgtcagaaataacagtgttaggaacgcgacaccagattgtcattaatgaggctgtagatcttgctaaacgtttctgtgatgggtcagccccccgcacaatcaatggttgtcttagaacattcgtcaatggccttgaagaatctggtttaactccagctttggggaaacagaaagcggtcactatcagtattaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

307

Amino Acids

33.55

Weight (kDa)

6.25

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 154
AccI GTMKAC 1 cut(s) 570
AccII CGCG 1 cut(s) 754
AciI CCGC 5 cut(s) 196, 306, 539, 820, 902
AclI AACGTT 1 cut(s) 796
AclWI GGATC 1 cut(s) 138
AcsI RAATTY 1 cut(s) 413
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 3 cut(s) 154, 721, 887
AgsI TTSAA 4 cut(s) 377, 479, 488, 863
AhdI GACNNNNNGTC 1 cut(s) 467
AjnI CCWGG 1 cut(s) 685
Alw21I GWGCWC 2 cut(s) 318, 504
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 1 cut(s) 856
ApeKI GCWGC 4 cut(s) 30, 212, 294, 551
ApoI RAATTY 1 cut(s) 413
AseI ATTAAT 1 cut(s) 771
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 2 cut(s) 405, 406
AsuII TTCGAA 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 461
BanII GRGCYC 2 cut(s) 318, 504
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 2 cut(s) 318, 504
BbvI GCAGC 4 cut(s) 17, 199, 306, 563
BccI CCATC 2 cut(s) 156, 800
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 687
BcnI CCSGG 2 cut(s) 405, 406
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 780
BglII AGATCT 1 cut(s) 784
BisI GCNGC 5 cut(s) 31, 213, 295, 306, 552
BlsI GCNGC 5 cut(s) 32, 214, 296, 307, 553
Bme1390I CCNGG 3 cut(s) 405, 406, 687
Bme18I GGWCC 1 cut(s) 461
BmeRI GACNNNNNGTC 1 cut(s) 467
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 2 cut(s) 355, 463
BmrFI CCNGG 3 cut(s) 405, 406, 687
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 720
BmuI ACTGGG 1 cut(s) 356
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 864
Bpu14I TTCGAA 1 cut(s) 184
BpuMI CCSGG 2 cut(s) 405, 406
BsaJI CCNNGG 1 cut(s) 404
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 721, 887
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 1 cut(s) 732
BseBI CCWGG 1 cut(s) 687
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 3 cut(s) 104, 685, 711
BseLI CCNNNNNNNGG 3 cut(s) 154, 721, 887
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 2 cut(s) 34, 40
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 4 cut(s) 25, 64, 91, 546
BseXI GCAGC 4 cut(s) 17, 199, 306, 563
Bsh1236I CGCG 1 cut(s) 754
BshFI GGCC 1 cut(s) 858
BsiHKAI GWGCWC 2 cut(s) 318, 504
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 405
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 3 cut(s) 154, 721, 887
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 1 cut(s) 858
BsoBI CYCGRG 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 318, 504
Bsp143I GATC 2 cut(s) 143, 784
BspACI CCGC 5 cut(s) 196, 306, 539, 820, 902
BspANI GGCC 1 cut(s) 858
BspCNI CTCAG 2 cut(s) 33, 39
BspFNI CGCG 1 cut(s) 754
BspLI GGNNCC 2 cut(s) 355, 463
BspPI GGATC 1 cut(s) 138
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 404
BssMI GATC 2 cut(s) 143, 784
BssNAI GTATAC 1 cut(s) 571
BssSI CACGAG 1 cut(s) 152
Bst1107I GTATAC 1 cut(s) 571
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 5 cut(s) 181, 475, 484, 635, 742
Bst6I CTCTTC 2 cut(s) 48, 115
BstAPI GCANNNNNTGC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 14, 318
BstDEI CTNAG 5 cut(s) 20, 26, 80, 346, 839
BstF5I GGATG 3 cut(s) 104, 685, 711
BstFNI CGCG 1 cut(s) 754
BstKTI GATC 2 cut(s) 146, 787
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 2 cut(s) 143, 784
BstMWI GCNNNNNNNGC 3 cut(s) 291, 351, 536
BstNI CCWGG 1 cut(s) 687
BstSCI CCNGG 3 cut(s) 403, 404, 685
BstSFI CTRYAG 1 cut(s) 780
BstUI CGCG 1 cut(s) 754
BstV1I GCAGC 4 cut(s) 17, 199, 306, 563
BstX2I RGATCY 2 cut(s) 143, 784
BstYI RGATCY 2 cut(s) 143, 784
BstZ17I GTATAC 1 cut(s) 571
BsuRI GGCC 1 cut(s) 858
BtsCI GGATG 3 cut(s) 104, 685, 711
BtsIMutI CAGTG 2 cut(s) 177, 747
Cac8I GCNNGC 2 cut(s) 14, 318
Cfr13I GGNCC 1 cut(s) 461
Cfr9I CCCGGG 1 cut(s) 404
Csp6I GTAC 1 cut(s) 365
CviAII CATG 2 cut(s) 448, 638
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 5 cut(s) 20, 26, 80, 346, 839
DpnI GATC 2 cut(s) 145, 786
DpnII GATC 2 cut(s) 143, 784
DriI GACNNNNNGTC 1 cut(s) 467
Eam1104I CTCTTC 2 cut(s) 48, 115
Eam1105I GACNNNNNGTC 1 cut(s) 467
EarI CTCTTC 2 cut(s) 48, 115
Ecl136II GAGCTC 2 cut(s) 316, 502
Eco24I GRGCYC 2 cut(s) 318, 504
Eco47I GGWCC 1 cut(s) 461
Eco53kI GAGCTC 2 cut(s) 316, 502
Eco88I CYCGRG 1 cut(s) 404
EcoICRI GAGCTC 2 cut(s) 316, 502
EcoO109I RGGNCCY 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 685
EcoT38I GRGCYC 2 cut(s) 318, 504
FaeI CATG 2 cut(s) 451, 641
FaiI YATR 8 cut(s) 260, 336, 411, 441, 449, 571, 620, 639
FaqI GGGAC 1 cut(s) 644
FatI CATG 2 cut(s) 447, 637
FauI CCCGC 1 cut(s) 827
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 31, 213, 295, 306, 552
FokI GGATG 3 cut(s) 91, 692, 698
FriOI GRGCYC 2 cut(s) 318, 504
Fsp4HI GCNGC 5 cut(s) 31, 213, 295, 306, 552
FspBI CTAG 1 cut(s) 548
GluI GCNGC 5 cut(s) 31, 213, 295, 306, 552
GsuI CTGGAG 1 cut(s) 864
HaeIII GGCC 1 cut(s) 858
HapII CCGG 1 cut(s) 405
Hin1II CATG 2 cut(s) 451, 641
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 4 cut(s) 248, 524, 696, 866
HpaII CCGG 1 cut(s) 405
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 4 cut(s) 247, 358, 458, 733
Hpy188III TCNNGA 3 cut(s) 230, 252, 398
Hpy8I GTNNAC 1 cut(s) 571
Hpy99I CGWCG 2 cut(s) 221, 399
HpyAV CCTTC 4 cut(s) 75, 344, 670, 725
HpyCH4III ACNGT 5 cut(s) 181, 475, 484, 635, 742
HpyCH4IV ACGT 2 cut(s) 206, 796
HpyCH4V TGCA 5 cut(s) 294, 530, 596, 614, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 291, 351, 536
HpyF3I CTNAG 5 cut(s) 20, 26, 80, 346, 839
HpySE526I ACGT 2 cut(s) 206, 796
Hsp92II CATG 2 cut(s) 451, 641
Kzo9I GATC 2 cut(s) 143, 784
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 5 cut(s) 38, 359, 507, 559, 703
Lsp1109I GCAGC 4 cut(s) 17, 199, 306, 563
LweI GCATC 1 cut(s) 720
MaeI CTAG 1 cut(s) 548
MaeII ACGT 2 cut(s) 206, 796
MaeIII GTNAC 2 cut(s) 469, 904
MalI GATC 2 cut(s) 145, 786
MboI GATC 2 cut(s) 143, 784
MboII GAAGA 8 cut(s) 35, 102, 105, 108, 111, 114, 500, 875
MflI RGATCY 2 cut(s) 143, 784
MhlI GDGCHC 2 cut(s) 318, 504
MluCI AATT 3 cut(s) 49, 413, 670
MmeI TCCRAC 3 cut(s) 264, 381, 414
MnlI CCTC 9 cut(s) 46, 85, 112, 118, 199, 452, 567, 675, 769
MseI TTAA 6 cut(s) 53, 161, 587, 771, 875, 918
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 3 cut(s) 405, 406, 687
MvaI CCWGG 1 cut(s) 687
MvnI CGCG 1 cut(s) 754
MwoI GCNNNNNNNGC 3 cut(s) 291, 351, 536
NciI CCSGG 2 cut(s) 405, 406
NdeII GATC 2 cut(s) 143, 784
NlaIII CATG 2 cut(s) 451, 641
NlaIV GGNNCC 2 cut(s) 355, 463
NmuCI GTSAC 2 cut(s) 469, 904
NspV TTCGAA 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 4 cut(s) 248, 524, 696, 866
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 5 cut(s) 32, 214, 296, 307, 553
PpuMI RGGWCCY 1 cut(s) 461
PshBI ATTAAT 1 cut(s) 771
Psp124BI GAGCTC 2 cut(s) 318, 504
Psp1406I AACGTT 1 cut(s) 796
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 685
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 2 cut(s) 355, 463
PspPI GGNCC 1 cut(s) 461
PspPPI RGGWCCY 1 cut(s) 461
PsuI RGATCY 2 cut(s) 143, 784
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
SacI GAGCTC 2 cut(s) 318, 504
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 6 cut(s) 53, 161, 587, 771, 875, 918
SatI GCNGC 5 cut(s) 31, 213, 295, 306, 552
Sau3AI GATC 2 cut(s) 143, 784
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 3 cut(s) 405, 406, 687
SduI GDGCHC 2 cut(s) 318, 504
SfaNI GCATC 1 cut(s) 720
SfcI CTRYAG 1 cut(s) 780
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 1 cut(s) 461
SmaI CCCGGG 1 cut(s) 406
Sse9I AATT 3 cut(s) 49, 413, 670
SsiI CCGC 5 cut(s) 196, 306, 539, 820, 902
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 2 cut(s) 318, 504
StyD4I CCNGG 3 cut(s) 403, 404, 685
TaaI ACNGT 5 cut(s) 181, 475, 484, 635, 742
TaiI ACGT 2 cut(s) 209, 799
TaqI TCGA 5 cut(s) 184, 219, 231, 397, 534
TasI AATT 3 cut(s) 49, 413, 670
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 4 cut(s) 248, 524, 696, 866
Tru1I TTAA 6 cut(s) 53, 161, 587, 771, 875, 918
Tru9I TTAA 6 cut(s) 53, 161, 587, 771, 875, 918
TscAI CASTG 2 cut(s) 184, 747
TseFI GTSAC 2 cut(s) 469, 904
TseI GCWGC 4 cut(s) 30, 212, 294, 551
Tsp45I GTSAC 2 cut(s) 469, 904
TspDTI ATGAA 4 cut(s) 237, 401, 417, 426
TspGWI ACGGA 1 cut(s) 383
TspMI CCCGGG 1 cut(s) 404
TspRI CASTG 2 cut(s) 184, 747
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 461
VspI ATTAAT 1 cut(s) 771
XapI RAATTY 1 cut(s) 413
XmaI CCCGGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 570
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.