RchiOBHm_Chr5g0084001

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010441
Physical Location & Seq
Reverse (-)
89636216 .. 89639736
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35810

Sequence Viewer

Length: 912 bp
ATGGAGGGAAGCTCGCTTCTCAGTCTCAGCTGCTCCTCTGCTCTTCCCAATTTAATACCCACTCCTTCTTACTCCTCTCTAAGCTTTACTCTCTCCCACTCCTCATCCTCTTCTTCTTTGCTATTTGGTAAATCCCATCTCGTGGGTTTAATCCCAAGACCATTCACTGTTCGAACCTCTCTCCGCACTTCCACGTTCGCTGCCGTCGATGAAGCTCTCGAAAAGTCCAACTCTGATTCCAGAGATATGTTGCCCAAGATTGACAAGAGTGGCAGATTTTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTCTCCATTATTTATGCTTCCTGCTTAGAAGGTTCCGACCCAGTACGCTTATTTGAAAAGCGAATGAATGTCCGTCGAGAACCCGGGTATGAATTTGACAAGGCTTCATTGTTGGAATATAATCCCATGAGCTTCGGAGGACCCCCTGTCACAGTTCAAACTGTTGAAGAAGCAGATGAGCTCCTGCTCAATGACGAAAAGGAATCTGCAATCGAAGCGGAAGTTCTTGCAGCTCCTCCAAAGTTGGTATACAGCAAACTGATTTTGAGATTCGCAAGGAAACTTTTGGTTGCAGTTATGGATAAATGGGACAGTCATGTGCTTGTCATTGACAGAGTTGCCCCTCCAAATTGGAAGGATGAGCCAGGAAGCAGAATCTTGGAGCTTTGCATCCTTCACTTGGCAATGTCAGAAATAACAGTGTTAGGAACGCGACACCAGATTGTCATTAATGAGGCTGTAGATCTTGCTAAACGTTTCTGTGATGGGTCAGCCCCCCGCACAATCAATGGTTGTCTTAGAACATTCGTCAATGGCCTTGAAGAATCTGGTTTAACTCCAGCTTTGGGGAAACAGAAAGCGGTCACTATCAGTATTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

303

Amino Acids

33.16

Weight (kDa)

6.25

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusB PF01029 189 - 280 3.9e-08 NusB family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 1 cut(s) 558
AccII CGCG 1 cut(s) 742
AciI CCGC 5 cut(s) 184, 294, 527, 808, 890
AclI AACGTT 1 cut(s) 784
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 1 cut(s) 354
AfiI CCNNNNNNNGG 3 cut(s) 142, 709, 875
AgsI TTSAA 4 cut(s) 365, 467, 476, 851
AhdI GACNNNNNGTC 1 cut(s) 455
AjnI CCWGG 1 cut(s) 673
Alw21I GWGCWC 2 cut(s) 306, 492
Alw26I GTCTC 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 392
AoxI GGCC 1 cut(s) 844
ApeKI GCWGC 4 cut(s) 30, 200, 282, 539
ApoI RAATTY 1 cut(s) 401
AseI ATTAAT 1 cut(s) 759
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 2 cut(s) 393, 394
AsuII TTCGAA 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 449
BanII GRGCYC 2 cut(s) 306, 492
BauI CACGAG 1 cut(s) 140
Bbv12I GWGCWC 2 cut(s) 306, 492
BbvI GCAGC 4 cut(s) 17, 187, 294, 551
BccI CCATC 2 cut(s) 144, 788
BceAI ACGGC 1 cut(s) 188
BciT130I CCWGG 1 cut(s) 675
BcnI CCSGG 2 cut(s) 393, 394
BcoDI GTCTC 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 768
BglII AGATCT 1 cut(s) 772
BisI GCNGC 5 cut(s) 31, 201, 283, 294, 540
BlsI GCNGC 5 cut(s) 32, 202, 284, 295, 541
Bme1390I CCNGG 3 cut(s) 393, 394, 675
Bme18I GGWCC 1 cut(s) 449
BmeRI GACNNNNNGTC 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 2 cut(s) 343, 451
BmrFI CCNGG 3 cut(s) 393, 394, 675
BmrI ACTGGG 1 cut(s) 344
BmsI GCATC 1 cut(s) 708
BmuI ACTGGG 1 cut(s) 344
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 852
Bpu14I TTCGAA 1 cut(s) 172
BpuMI CCSGG 2 cut(s) 393, 394
BsaJI CCNNGG 1 cut(s) 392
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 709, 875
Bse1I ACTGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 1 cut(s) 675
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 3 cut(s) 104, 673, 699
BseLI CCNNNNNNNGG 3 cut(s) 142, 709, 875
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 2 cut(s) 34, 40
BseNI ACTGG 1 cut(s) 350
BseRI GAGGAG 4 cut(s) 25, 64, 91, 534
BseXI GCAGC 4 cut(s) 17, 187, 294, 551
Bsh1236I CGCG 1 cut(s) 742
BshFI GGCC 1 cut(s) 846
BsiHKAI GWGCWC 2 cut(s) 306, 492
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 1 cut(s) 393
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 3 cut(s) 142, 709, 875
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 1 cut(s) 846
BsoBI CYCGRG 1 cut(s) 392
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 306, 492
Bsp143I GATC 1 cut(s) 772
BspACI CCGC 5 cut(s) 184, 294, 527, 808, 890
BspANI GGCC 1 cut(s) 846
BspCNI CTCAG 2 cut(s) 33, 39
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 2 cut(s) 343, 451
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 1 cut(s) 350
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 1 cut(s) 772
BssNAI GTATAC 1 cut(s) 559
BssSI CACGAG 1 cut(s) 140
Bst1107I GTATAC 1 cut(s) 559
Bst2BI CACGAG 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 5 cut(s) 169, 463, 472, 623, 730
Bst6I CTCTTC 2 cut(s) 48, 115
BstAPI GCANNNNNTGC 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 14, 306
BstDEI CTNAG 5 cut(s) 20, 26, 80, 334, 827
BstF5I GGATG 3 cut(s) 104, 673, 699
BstFNI CGCG 1 cut(s) 742
BstKTI GATC 1 cut(s) 775
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 1 cut(s) 772
BstMWI GCNNNNNNNGC 2 cut(s) 279, 524
BstNI CCWGG 1 cut(s) 675
BstSCI CCNGG 3 cut(s) 391, 392, 673
BstSFI CTRYAG 1 cut(s) 768
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 4 cut(s) 17, 187, 294, 551
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
BstZ17I GTATAC 1 cut(s) 559
BsuRI GGCC 1 cut(s) 846
BtsCI GGATG 3 cut(s) 104, 673, 699
BtsIMutI CAGTG 2 cut(s) 165, 735
Cac8I GCNNGC 2 cut(s) 14, 306
Cfr13I GGNCC 1 cut(s) 449
Cfr9I CCCGGG 1 cut(s) 392
Csp6I GTAC 1 cut(s) 353
CviAII CATG 2 cut(s) 436, 626
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 5 cut(s) 20, 26, 80, 334, 827
DpnI GATC 1 cut(s) 774
DpnII GATC 1 cut(s) 772
DriI GACNNNNNGTC 1 cut(s) 455
Eam1104I CTCTTC 2 cut(s) 48, 115
Eam1105I GACNNNNNGTC 1 cut(s) 455
EarI CTCTTC 2 cut(s) 48, 115
Ecl136II GAGCTC 2 cut(s) 304, 490
Eco24I GRGCYC 2 cut(s) 306, 492
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 2 cut(s) 304, 490
Eco88I CYCGRG 1 cut(s) 392
EcoICRI GAGCTC 2 cut(s) 304, 490
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 673
EcoT38I GRGCYC 2 cut(s) 306, 492
FaeI CATG 2 cut(s) 439, 629
FaiI YATR 8 cut(s) 248, 324, 399, 429, 437, 559, 608, 627
FaqI GGGAC 1 cut(s) 632
FatI CATG 2 cut(s) 435, 625
FauI CCCGC 1 cut(s) 815
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 5 cut(s) 31, 201, 283, 294, 540
FokI GGATG 3 cut(s) 91, 680, 686
FriOI GRGCYC 2 cut(s) 306, 492
Fsp4HI GCNGC 5 cut(s) 31, 201, 283, 294, 540
GluI GCNGC 5 cut(s) 31, 201, 283, 294, 540
GsuI CTGGAG 1 cut(s) 852
HaeIII GGCC 1 cut(s) 846
HapII CCGG 1 cut(s) 393
Hin1II CATG 2 cut(s) 439, 629
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 5 cut(s) 236, 512, 579, 684, 854
HpaII CCGG 1 cut(s) 393
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 4 cut(s) 235, 346, 446, 721
Hpy188III TCNNGA 3 cut(s) 218, 240, 386
Hpy8I GTNNAC 1 cut(s) 559
Hpy99I CGWCG 2 cut(s) 209, 387
HpyAV CCTTC 4 cut(s) 75, 332, 658, 713
HpyCH4III ACNGT 5 cut(s) 169, 463, 472, 623, 730
HpyCH4IV ACGT 2 cut(s) 194, 784
HpyCH4V TGCA 5 cut(s) 282, 518, 539, 602, 699
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 524
HpyF3I CTNAG 5 cut(s) 20, 26, 80, 334, 827
HpySE526I ACGT 2 cut(s) 194, 784
Hsp92II CATG 2 cut(s) 439, 629
Kzo9I GATC 1 cut(s) 772
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 4 cut(s) 38, 495, 547, 691
Lsp1109I GCAGC 4 cut(s) 17, 187, 294, 551
LweI GCATC 1 cut(s) 708
MaeII ACGT 2 cut(s) 194, 784
MaeIII GTNAC 2 cut(s) 457, 892
MalI GATC 1 cut(s) 774
MboI GATC 1 cut(s) 772
MboII GAAGA 5 cut(s) 35, 102, 105, 488, 863
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 2 cut(s) 306, 492
MluCI AATT 3 cut(s) 49, 401, 658
MmeI TCCRAC 3 cut(s) 252, 369, 402
MnlI CCTC 9 cut(s) 46, 85, 112, 118, 187, 440, 555, 663, 757
MseI TTAA 5 cut(s) 53, 149, 759, 863, 906
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 3 cut(s) 393, 394, 675
MvaI CCWGG 1 cut(s) 675
MvnI CGCG 1 cut(s) 742
MwoI GCNNNNNNNGC 2 cut(s) 279, 524
NciI CCSGG 2 cut(s) 393, 394
NdeII GATC 1 cut(s) 772
NlaIII CATG 2 cut(s) 439, 629
NlaIV GGNNCC 2 cut(s) 343, 451
NmuCI GTSAC 2 cut(s) 457, 892
NspV TTCGAA 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 5 cut(s) 236, 512, 579, 684, 854
PflMI CCANNNNNTGG 1 cut(s) 142
PkrI GCNGC 5 cut(s) 32, 202, 284, 295, 541
PpuMI RGGWCCY 1 cut(s) 449
PshBI ATTAAT 1 cut(s) 759
Psp124BI GAGCTC 2 cut(s) 306, 492
Psp1406I AACGTT 1 cut(s) 784
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 2 cut(s) 343, 451
PspPI GGNCC 1 cut(s) 449
PspPPI RGGWCCY 1 cut(s) 449
PsuI RGATCY 1 cut(s) 772
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
SacI GAGCTC 2 cut(s) 306, 492
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 5 cut(s) 53, 149, 759, 863, 906
SatI GCNGC 5 cut(s) 31, 201, 283, 294, 540
Sau3AI GATC 1 cut(s) 772
Sau96I GGNCC 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 393, 394, 675
SduI GDGCHC 2 cut(s) 306, 492
SfaNI GCATC 1 cut(s) 708
SfcI CTRYAG 1 cut(s) 768
SfuI TTCGAA 1 cut(s) 172
SinI GGWCC 1 cut(s) 449
SmaI CCCGGG 1 cut(s) 394
Sse9I AATT 3 cut(s) 49, 401, 658
SsiI CCGC 5 cut(s) 184, 294, 527, 808, 890
SstI GAGCTC 2 cut(s) 306, 492
StyD4I CCNGG 3 cut(s) 391, 392, 673
TaaI ACNGT 5 cut(s) 169, 463, 472, 623, 730
TaiI ACGT 2 cut(s) 197, 787
TaqI TCGA 5 cut(s) 172, 207, 219, 385, 522
TasI AATT 3 cut(s) 49, 401, 658
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 5 cut(s) 236, 512, 579, 684, 854
Tru1I TTAA 5 cut(s) 53, 149, 759, 863, 906
Tru9I TTAA 5 cut(s) 53, 149, 759, 863, 906
TscAI CASTG 2 cut(s) 172, 735
TseFI GTSAC 2 cut(s) 457, 892
TseI GCWGC 4 cut(s) 30, 200, 282, 539
Tsp45I GTSAC 2 cut(s) 457, 892
TspDTI ATGAA 4 cut(s) 225, 389, 405, 414
TspGWI ACGGA 1 cut(s) 371
TspMI CCCGGG 1 cut(s) 392
TspRI CASTG 2 cut(s) 172, 735
Van91I CCANNNNNTGG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 449
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 1 cut(s) 401
XmaI CCCGGG 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.