Rh5AG540900

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010441
Physical Location & Seq
Reverse (-)
92868039 .. 92871235
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG540900.1

Sequence Viewer

Length: 918 bp
ATGGAGGGAAGCTCGCTTCTCAGTCTCAGCTGCTCCTCTGCTCTTCTCAATTTAATACCCACTCCTTCTTACTCCTCTCTAAGCTTTACTCTCTCCCACTCCTCATCCTCTTCTTCTTCTCCTTTGCTATTTGGTAGATCCCATCTCGTGGGTTTAATCCCAAGACCATTCACTGTTCGAACCTCTCTCCGCACTTCCACGTTCGCTGCCGTCGATGAAGCTCTCGAAAAGTCCACCTCTGATTCCAGAGATATGTTGCCCAAGATTGACAAGAGTGGCAGATTTTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTCTCCATTATTTATGCTTCCTGCTTAGAAGGTTCCGACCCAGTACGCTTATTTGAAAAGCGAATGAATGTCCGTCGAGAACCCGGGTATGAATTTGACAAGGCTTCATTGTTGGAATATAATCCCATGAGCTTCGGAGGACCCCCTGTCACAGTTCAAACTGTTGAAGAAGCAGATGAGCTCCTGCTCAATGACGAAAAGGAATCTGCAATCGAAGCGGAAGTTCTTGCAGCTCCTCCAAAGTTGGTATACAGCAAACTGATTTTAAGATTTGCAAGGAAACTTTTGGTTGCAGTTATGGATAAATGGGACAGTCATGTGCTTGTCATTGACAGAGTTGCCCCTCCAAATTGGAAGGATGAGCCAGGAAGCAGAATCTTGGAGCTTTGCATCCTTCACTTGGCAATGTCAGAAATAACAGTGTTAGGAACGCGACACCAGATTGTCATTAATGAGGCTGTAGATCTTGCTAAACGTTTCTGTGATGGGTCAGCCCCCCGCACAATCAATGGTTGTCTTAGAACATTCGTCAATGGCCTTGAAGAATCTGGTTTAACTCCAGCTTTGGGGAAACAGAAAGCTGTCACTATCAGTATTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

305

Amino Acids

33.38

Weight (kDa)

6.25

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusB PF01029 191 - 282 4e-08 NusB family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 148
AccI GTMKAC 1 cut(s) 564
AccII CGCG 1 cut(s) 748
AciI CCGC 4 cut(s) 190, 300, 533, 814
AclI AACGTT 1 cut(s) 790
AclWI GGATC 1 cut(s) 132
AcsI RAATTY 1 cut(s) 407
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 3 cut(s) 148, 715, 881
AgsI TTSAA 4 cut(s) 371, 473, 482, 857
AhdI GACNNNNNGTC 1 cut(s) 461
AjnI CCWGG 1 cut(s) 679
Alw21I GWGCWC 2 cut(s) 312, 498
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 132
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 1 cut(s) 850
ApeKI GCWGC 4 cut(s) 30, 206, 288, 545
ApoI RAATTY 1 cut(s) 407
AseI ATTAAT 1 cut(s) 765
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 2 cut(s) 399, 400
AsuII TTCGAA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 1 cut(s) 455
BanII GRGCYC 2 cut(s) 312, 498
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 312, 498
BbvI GCAGC 4 cut(s) 17, 193, 300, 557
BccI CCATC 2 cut(s) 150, 794
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 1 cut(s) 681
BcnI CCSGG 2 cut(s) 399, 400
BcoDI GTCTC 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 774
BglII AGATCT 1 cut(s) 778
BisI GCNGC 5 cut(s) 31, 207, 289, 300, 546
BlsI GCNGC 5 cut(s) 32, 208, 290, 301, 547
Bme1390I CCNGG 3 cut(s) 399, 400, 681
Bme18I GGWCC 1 cut(s) 455
BmeRI GACNNNNNGTC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 349, 457
BmrFI CCNGG 3 cut(s) 399, 400, 681
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 714
BmuI ACTGGG 1 cut(s) 350
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 858
Bpu14I TTCGAA 1 cut(s) 178
BpuMI CCSGG 2 cut(s) 399, 400
BsaJI CCNNGG 1 cut(s) 398
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 715, 881
Bse1I ACTGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 726
BseBI CCWGG 1 cut(s) 681
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 3 cut(s) 104, 679, 705
BseLI CCNNNNNNNGG 3 cut(s) 148, 715, 881
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 2 cut(s) 34, 40
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 4 cut(s) 25, 64, 91, 540
BseXI GCAGC 4 cut(s) 17, 193, 300, 557
Bsh1236I CGCG 1 cut(s) 748
BshFI GGCC 1 cut(s) 852
BsiHKAI GWGCWC 2 cut(s) 312, 498
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 3 cut(s) 148, 715, 881
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 1 cut(s) 852
BsoBI CYCGRG 1 cut(s) 398
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 312, 498
Bsp143I GATC 2 cut(s) 137, 778
BspACI CCGC 4 cut(s) 190, 300, 533, 814
BspANI GGCC 1 cut(s) 852
BspCNI CTCAG 2 cut(s) 33, 39
BspFNI CGCG 1 cut(s) 748
BspLI GGNNCC 2 cut(s) 349, 457
BspPI GGATC 1 cut(s) 132
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 726
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 2 cut(s) 137, 778
BssNAI GTATAC 1 cut(s) 565
BssSI CACGAG 1 cut(s) 146
Bst1107I GTATAC 1 cut(s) 565
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 681
Bst4CI ACNGT 5 cut(s) 175, 469, 478, 629, 736
Bst6I CTCTTC 2 cut(s) 48, 115
BstAPI GCANNNNNTGC 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 14, 312
BstDEI CTNAG 5 cut(s) 20, 26, 80, 340, 833
BstF5I GGATG 3 cut(s) 104, 679, 705
BstFNI CGCG 1 cut(s) 748
BstKTI GATC 2 cut(s) 140, 781
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 2 cut(s) 137, 778
BstMWI GCNNNNNNNGC 2 cut(s) 285, 530
BstNI CCWGG 1 cut(s) 681
BstSCI CCNGG 3 cut(s) 397, 398, 679
BstSFI CTRYAG 1 cut(s) 774
BstUI CGCG 1 cut(s) 748
BstV1I GCAGC 4 cut(s) 17, 193, 300, 557
BstX2I RGATCY 2 cut(s) 137, 778
BstYI RGATCY 2 cut(s) 137, 778
BstZ17I GTATAC 1 cut(s) 565
BsuRI GGCC 1 cut(s) 852
BtsCI GGATG 3 cut(s) 104, 679, 705
BtsIMutI CAGTG 2 cut(s) 171, 741
Cac8I GCNNGC 2 cut(s) 14, 312
Cfr13I GGNCC 1 cut(s) 455
Cfr9I CCCGGG 1 cut(s) 398
Csp6I GTAC 1 cut(s) 359
CviAII CATG 2 cut(s) 442, 632
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 5 cut(s) 20, 26, 80, 340, 833
DpnI GATC 2 cut(s) 139, 780
DpnII GATC 2 cut(s) 137, 778
DriI GACNNNNNGTC 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 48, 115
Eam1105I GACNNNNNGTC 1 cut(s) 461
EarI CTCTTC 2 cut(s) 48, 115
Ecl136II GAGCTC 2 cut(s) 310, 496
Eco24I GRGCYC 2 cut(s) 312, 498
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 2 cut(s) 310, 496
Eco88I CYCGRG 1 cut(s) 398
EcoICRI GAGCTC 2 cut(s) 310, 496
EcoO109I RGGNCCY 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 679
EcoT38I GRGCYC 2 cut(s) 312, 498
FaeI CATG 2 cut(s) 445, 635
FaiI YATR 8 cut(s) 254, 330, 405, 435, 443, 565, 614, 633
FaqI GGGAC 1 cut(s) 638
FatI CATG 2 cut(s) 441, 631
FauI CCCGC 1 cut(s) 821
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 5 cut(s) 31, 207, 289, 300, 546
FokI GGATG 3 cut(s) 91, 686, 692
FriOI GRGCYC 2 cut(s) 312, 498
Fsp4HI GCNGC 5 cut(s) 31, 207, 289, 300, 546
GluI GCNGC 5 cut(s) 31, 207, 289, 300, 546
GsuI CTGGAG 1 cut(s) 858
HaeIII GGCC 1 cut(s) 852
HapII CCGG 1 cut(s) 399
Hin1II CATG 2 cut(s) 445, 635
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 4 cut(s) 242, 518, 690, 860
HpaII CCGG 1 cut(s) 399
Hpy166II GTNNAC 2 cut(s) 234, 565
Hpy188I TCNGA 4 cut(s) 241, 352, 452, 727
Hpy188III TCNNGA 3 cut(s) 224, 246, 392
Hpy8I GTNNAC 2 cut(s) 234, 565
Hpy99I CGWCG 2 cut(s) 215, 393
HpyAV CCTTC 4 cut(s) 75, 338, 664, 719
HpyCH4III ACNGT 5 cut(s) 175, 469, 478, 629, 736
HpyCH4IV ACGT 2 cut(s) 200, 790
HpyCH4V TGCA 6 cut(s) 288, 524, 545, 590, 608, 705
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 530
HpyF3I CTNAG 5 cut(s) 20, 26, 80, 340, 833
HpySE526I ACGT 2 cut(s) 200, 790
Hsp92II CATG 2 cut(s) 445, 635
Kzo9I GATC 2 cut(s) 137, 778
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 4 cut(s) 38, 501, 553, 697
Lsp1109I GCAGC 4 cut(s) 17, 193, 300, 557
LweI GCATC 1 cut(s) 714
MaeII ACGT 2 cut(s) 200, 790
MaeIII GTNAC 2 cut(s) 463, 898
MalI GATC 2 cut(s) 139, 780
MboI GATC 2 cut(s) 137, 778
MboII GAAGA 6 cut(s) 35, 102, 105, 108, 494, 869
MflI RGATCY 2 cut(s) 137, 778
MhlI GDGCHC 2 cut(s) 312, 498
MluCI AATT 3 cut(s) 49, 407, 664
MmeI TCCRAC 2 cut(s) 375, 408
MseI TTAA 6 cut(s) 53, 155, 581, 765, 869, 912
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 3 cut(s) 399, 400, 681
MvaI CCWGG 1 cut(s) 681
MvnI CGCG 1 cut(s) 748
MwoI GCNNNNNNNGC 2 cut(s) 285, 530
NciI CCSGG 2 cut(s) 399, 400
NdeII GATC 2 cut(s) 137, 778
NlaIII CATG 2 cut(s) 445, 635
NlaIV GGNNCC 2 cut(s) 349, 457
NmuCI GTSAC 2 cut(s) 463, 898
NspV TTCGAA 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 48
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 4 cut(s) 242, 518, 690, 860
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 5 cut(s) 32, 208, 290, 301, 547
PpuMI RGGWCCY 1 cut(s) 455
PshBI ATTAAT 1 cut(s) 765
Psp124BI GAGCTC 2 cut(s) 312, 498
Psp1406I AACGTT 1 cut(s) 790
Psp5II RGGWCCY 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 679
PspGI CCWGG 1 cut(s) 679
PspN4I GGNNCC 2 cut(s) 349, 457
PspPI GGNCC 1 cut(s) 455
PspPPI RGGWCCY 1 cut(s) 455
PsuI RGATCY 2 cut(s) 137, 778
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SacI GAGCTC 2 cut(s) 312, 498
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 6 cut(s) 53, 155, 581, 765, 869, 912
SatI GCNGC 5 cut(s) 31, 207, 289, 300, 546
Sau3AI GATC 2 cut(s) 137, 778
Sau96I GGNCC 1 cut(s) 455
ScrFI CCNGG 3 cut(s) 399, 400, 681
SduI GDGCHC 2 cut(s) 312, 498
SfaNI GCATC 1 cut(s) 714
SfcI CTRYAG 1 cut(s) 774
SfuI TTCGAA 1 cut(s) 178
SinI GGWCC 1 cut(s) 455
SmaI CCCGGG 1 cut(s) 400
Sse9I AATT 3 cut(s) 49, 407, 664
SsiI CCGC 4 cut(s) 190, 300, 533, 814
SstI GAGCTC 2 cut(s) 312, 498
StyD4I CCNGG 3 cut(s) 397, 398, 679
TaaI ACNGT 5 cut(s) 175, 469, 478, 629, 736
TaiI ACGT 2 cut(s) 203, 793
TaqI TCGA 5 cut(s) 178, 213, 225, 391, 528
TasI AATT 3 cut(s) 49, 407, 664
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 4 cut(s) 242, 518, 690, 860
Tru1I TTAA 6 cut(s) 53, 155, 581, 765, 869, 912
Tru9I TTAA 6 cut(s) 53, 155, 581, 765, 869, 912
TscAI CASTG 2 cut(s) 178, 741
TseFI GTSAC 2 cut(s) 463, 898
TseI GCWGC 4 cut(s) 30, 206, 288, 545
Tsp45I GTSAC 2 cut(s) 463, 898
TspDTI ATGAA 4 cut(s) 231, 395, 411, 420
TspGWI ACGGA 1 cut(s) 377
TspMI CCCGGG 1 cut(s) 398
TspRI CASTG 2 cut(s) 178, 741
Van91I CCANNNNNTGG 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 455
VspI ATTAAT 1 cut(s) 765
XapI RAATTY 1 cut(s) 407
XmaI CCCGGG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.