Rorug05G0488200

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010441
Physical Location & Seq
Reverse (-)
67152912 .. 67153711
800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0488200.1

Sequence Viewer

Length: 708 bp
ATGGCTTCTTCAGCAATGGCGCTTTCCTCTCCTTCTCTAGCTGGCCAAGCTGTGAAGCTCAGTCCATCTACATCTAATCTCCTTGGTGAGGGCAGAGTCACAATGCGCAAAACCGCAGCCAAGTCCAAAACTGTTTCAGGCAGCCCATGGTACGGTGCAGATCGTGTCAAGTACTTGGGCCCATTTTCCGGTGAGGCTCCTTCATACCTCACCGGTGAGTTTCCCGGGGACTACGGTTGGGACACTGCTGGGCTATCAGCGGATCTCGAGACCTTTGCCAAAAACCGTGAGCTTGAGGTCATCCATTCCAGATGGGCAATGCTCGGCGCCTTGGGTTGTGTTTTCCCTGAACTCCTAGCCCGCAACGGAGTCAAGTTCGGCGAAGCCGTTTGGTTCAAGGCCGGAGCCCAGATATTCAGTGAGGGTGGGCTACAGAGTTACCGGTGGGCACTCGGTGAGGTGGTAGACCCATTGTACCCCGGCGGGAGTTTCGACCCGTTAGGCCTTGCAGAAGATCCAGAAGCTTTTGCTGAGCTAAAGGTAAAGGAGCTCAAGAATGGAAGACTAGCTATGTTTTCCATGTTCGGGTTTTTCGTACAAGCCATTGTTACCGGGAAGGGGCCAATCAAGAACCTGGCTGACCACTTAGCTGACCCTGTTAACAACAATGCTTGGTCATATGCCACCAACTTTGTTCCCGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

235

Amino Acids

25.13

Weight (kDa)

6.16

Isoelectric Point (pI)

25.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroa_b-bind PF00504 66 - 202 9e-43 Chlorophyll A-B binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 465
AciI CCGC 4 cut(s) 114, 260, 361, 483
AclWI GGATC 2 cut(s) 270, 509
AcoI YGGCCR 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 327
AdeI CACNNNGTG 1 cut(s) 455
AfaI GTAC 4 cut(s) 152, 173, 476, 597
AfiI CCNNNNNNNGG 5 cut(s) 88, 152, 188, 585, 618
AgeI ACCGGT 2 cut(s) 212, 441
AgsI TTSAA 1 cut(s) 397
AjnI CCWGG 1 cut(s) 633
AluBI AGCT 9 cut(s) 41, 50, 58, 292, 524, 535, 550, 569, 650
AluI AGCT 9 cut(s) 41, 50, 58, 292, 524, 535, 550, 569, 650
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 2 cut(s) 270, 509
Ama87I CYCGRG 2 cut(s) 224, 266
AoxI GGCC 5 cut(s) 43, 178, 399, 502, 620
ApaI GGGCCC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 116, 141
AsiGI ACCGGT 2 cut(s) 212, 441
AspLEI GCGC 3 cut(s) 22, 108, 329
AspS9I GGNCC 3 cut(s) 178, 179, 620
AsuC2I CCSGG 5 cut(s) 225, 226, 480, 613, 699
AsuHPI GGTGA 5 cut(s) 98, 202, 203, 227, 467
AvaI CYCGRG 2 cut(s) 224, 266
BaeGI GKGCMC 2 cut(s) 182, 451
BalI TGGCCA 1 cut(s) 45
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 3 cut(s) 182, 409, 552
BbsI GAAGAC 1 cut(s) 568
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 2 cut(s) 128, 153
BccI CCATC 2 cut(s) 73, 306
BceAI ACGGC 1 cut(s) 371
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 1 cut(s) 635
BcnI CCSGG 5 cut(s) 225, 226, 480, 613, 699
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 3 cut(s) 38, 356, 566
BfmI CTRYAG 1 cut(s) 431
BfoI RGCGCY 2 cut(s) 23, 330
BisI GCNGC 2 cut(s) 117, 142
BlpI GCTNAGC 1 cut(s) 531
BlsI GCNGC 2 cut(s) 118, 143
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 6 cut(s) 225, 226, 480, 613, 635, 699
BmeT110I CYCGRG 2 cut(s) 224, 266
BmgT120I GGNCC 3 cut(s) 178, 179, 620
BmiI GGNNCC 5 cut(s) 180, 198, 328, 406, 621
BmrFI CCNGG 6 cut(s) 225, 226, 480, 613, 635, 699
BpiI GAAGAC 1 cut(s) 568
Bpu1102I GCTNAGC 1 cut(s) 531
BpuEI CTTGAG 2 cut(s) 314, 536
BpuMI CCSGG 5 cut(s) 225, 226, 480, 613, 699
BsaHI GRCGYC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 6 cut(s) 82, 146, 224, 225, 330, 478
BsaWI WCCGGW 3 cut(s) 188, 212, 441
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 152, 188, 585, 618
Bse118I RCCGGY 2 cut(s) 212, 441
Bse3DI GCAATG 2 cut(s) 21, 324
BseBI CCWGG 1 cut(s) 635
BseDI CCNNGG 6 cut(s) 82, 146, 224, 225, 330, 478
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 5 cut(s) 88, 152, 188, 585, 618
BseMI GCAATG 2 cut(s) 21, 324
BseMII CTCAG 2 cut(s) 73, 522
BseSI GKGCMC 2 cut(s) 182, 451
BseXI GCAGC 2 cut(s) 128, 153
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 177
BshFI GGCC 5 cut(s) 45, 180, 401, 504, 622
BshNI GGYRCC 1 cut(s) 326
BshTI ACCGGT 2 cut(s) 212, 441
BsiHKAI GWGCWC 1 cut(s) 552
BsiHKCI CYCGRG 2 cut(s) 224, 266
BsiSI CCGG 8 cut(s) 189, 213, 225, 402, 442, 480, 612, 699
BslFI GGGAC 2 cut(s) 242, 254
BslI CCNNNNNNNGG 5 cut(s) 88, 152, 188, 585, 618
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 242, 254
BsnI GGCC 5 cut(s) 45, 180, 401, 504, 622
Bso31I GGTCTC 1 cut(s) 263
BsoBI CYCGRG 2 cut(s) 224, 266
Bsp120I GGGCCC 1 cut(s) 178
Bsp1286I GDGCHC 4 cut(s) 182, 409, 451, 552
Bsp143I GATC 3 cut(s) 160, 262, 514
Bsp1720I GCTNAGC 1 cut(s) 531
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 4 cut(s) 114, 260, 361, 483
BspANI GGCC 5 cut(s) 45, 180, 401, 504, 622
BspCNI CTCAG 2 cut(s) 72, 523
BspLI GGNNCC 5 cut(s) 180, 198, 328, 406, 621
BspPI GGATC 2 cut(s) 270, 509
BspT107I GGYRCC 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 2 cut(s) 21, 324
BsrFI RCCGGY 2 cut(s) 212, 441
BssAI RCCGGY 2 cut(s) 212, 441
BssECI CCNNGG 6 cut(s) 82, 146, 224, 225, 330, 478
BssMI GATC 3 cut(s) 160, 262, 514
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 3 cut(s) 82, 146, 330
Bst2UI CCWGG 1 cut(s) 635
Bst4CI ACNGT 4 cut(s) 133, 155, 236, 287
BstACI GRCGYC 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 43, 361
BstDEI CTNAG 3 cut(s) 59, 531, 646
BstDSI CCRYGG 1 cut(s) 146
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 1 cut(s) 300
BstH2I RGCGCY 2 cut(s) 23, 330
BstHHI GCGC 3 cut(s) 22, 108, 329
BstKTI GATC 3 cut(s) 163, 265, 517
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 3 cut(s) 160, 262, 514
BstMWI GCNNNNNNNGC 2 cut(s) 11, 47
BstNI CCWGG 1 cut(s) 635
BstSCI CCNGG 6 cut(s) 223, 224, 478, 611, 633, 697
BstSFI CTRYAG 1 cut(s) 431
BstSLI GKGCMC 2 cut(s) 182, 451
BstV1I GCAGC 2 cut(s) 128, 153
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 2 cut(s) 262, 514
BstYI RGATCY 2 cut(s) 262, 514
BsuRI GGCC 5 cut(s) 45, 180, 401, 504, 622
BtgI CCRYGG 1 cut(s) 146
BtsCI GGATG 1 cut(s) 300
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 243, 424
Cac8I GCNNGC 2 cut(s) 43, 361
CfoI GCGC 3 cut(s) 22, 108, 329
Cfr10I RCCGGY 2 cut(s) 212, 441
Cfr13I GGNCC 3 cut(s) 178, 179, 620
Cfr9I CCCGGG 1 cut(s) 224
Csp6I GTAC 4 cut(s) 151, 172, 475, 596
CspAI ACCGGT 2 cut(s) 212, 441
CspCI CAANNNNNGTGG 2 cut(s) 673, 708
CviAII CATG 2 cut(s) 147, 580
CviQI GTAC 4 cut(s) 151, 172, 475, 596
DdeI CTNAG 3 cut(s) 59, 531, 646
DinI GGCGCC 1 cut(s) 328
DpnI GATC 3 cut(s) 162, 264, 516
DpnII GATC 3 cut(s) 160, 262, 514
DraIII CACNNNGTG 1 cut(s) 455
EaeI YGGCCR 1 cut(s) 43
Ecl136II GAGCTC 1 cut(s) 550
Eco130I CCWWGG 3 cut(s) 82, 146, 330
Eco147I AGGCCT 1 cut(s) 504
Eco24I GRGCYC 3 cut(s) 182, 409, 552
Eco31I GGTCTC 1 cut(s) 263
Eco53kI GAGCTC 1 cut(s) 550
Eco88I CYCGRG 2 cut(s) 224, 266
EcoICRI GAGCTC 1 cut(s) 550
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 633
EcoT14I CCWWGG 3 cut(s) 82, 146, 330
EcoT38I GRGCYC 3 cut(s) 182, 409, 552
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
ErhI CCWWGG 3 cut(s) 82, 146, 330
FaeI CATG 2 cut(s) 150, 583
FaiI YATR 6 cut(s) 148, 205, 572, 581, 679, 681
FaqI GGGAC 2 cut(s) 242, 254
FatI CATG 2 cut(s) 146, 579
FauI CCCGC 2 cut(s) 368, 476
FauNDI CATATG 1 cut(s) 679
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 2 cut(s) 117, 142
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 3 cut(s) 182, 409, 552
Fsp4HI GCNGC 2 cut(s) 117, 142
FspBI CTAG 3 cut(s) 38, 356, 566
FspI TGCGCA 1 cut(s) 107
GlaI GCGC 3 cut(s) 21, 107, 328
GluI GCNGC 2 cut(s) 117, 142
GsaI CCCAGC 1 cut(s) 252
HaeII RGCGCY 2 cut(s) 23, 330
HaeIII GGCC 5 cut(s) 45, 180, 401, 504, 622
HapII CCGG 8 cut(s) 189, 213, 225, 402, 442, 480, 612, 699
HhaI GCGC 3 cut(s) 22, 108, 329
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 2 cut(s) 150, 583
Hin6I GCGC 3 cut(s) 20, 106, 327
HinP1I GCGC 3 cut(s) 20, 106, 327
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 2 cut(s) 96, 369
HpaI GTTAAC 1 cut(s) 661
HpaII CCGG 8 cut(s) 189, 213, 225, 402, 442, 480, 612, 699
HphI GGTGA 5 cut(s) 98, 202, 203, 227, 467
Hpy166II GTNNAC 2 cut(s) 466, 661
Hpy188III TCNNGA 6 cut(s) 266, 268, 309, 518, 553, 628
Hpy8I GTNNAC 2 cut(s) 466, 661
HpyAV CCTTC 3 cut(s) 42, 210, 610
HpyCH4III ACNGT 4 cut(s) 133, 155, 236, 287
HpyCH4V TGCA 2 cut(s) 158, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 47
HpyF3I CTNAG 3 cut(s) 59, 531, 646
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 2 cut(s) 150, 583
HspAI GCGC 3 cut(s) 20, 106, 327
KasI GGCGCC 1 cut(s) 326
KspAI GTTAAC 1 cut(s) 661
Kzo9I GATC 3 cut(s) 160, 262, 514
LmnI GCTCC 3 cut(s) 202, 404, 547
Lsp1109I GCAGC 2 cut(s) 128, 153
MaeI CTAG 3 cut(s) 38, 356, 566
MaeIII GTNAC 3 cut(s) 97, 437, 607
MalI GATC 3 cut(s) 162, 264, 516
MboI GATC 3 cut(s) 160, 262, 514
MboII GAAGA 2 cut(s) 524, 573
MflI RGATCY 2 cut(s) 262, 514
MhlI GDGCHC 4 cut(s) 182, 409, 451, 552
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 2 cut(s) 105, 378
MnlI CCTC 7 cut(s) 37, 82, 187, 218, 289, 415, 451
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 1 cut(s) 660
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 8 cut(s) 189, 213, 225, 402, 442, 480, 612, 699
MspR9I CCNGG 6 cut(s) 225, 226, 480, 613, 635, 699
MvaI CCWGG 1 cut(s) 635
MwoI GCNNNNNNNGC 2 cut(s) 11, 47
NarI GGCGCC 1 cut(s) 327
NciI CCSGG 5 cut(s) 225, 226, 480, 613, 699
NcoI CCATGG 1 cut(s) 146
NdeI CATATG 1 cut(s) 679
NdeII GATC 3 cut(s) 160, 262, 514
NlaIII CATG 2 cut(s) 150, 583
NlaIV GGNNCC 5 cut(s) 180, 198, 328, 406, 621
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 97
NsbI TGCGCA 1 cut(s) 107
PaeR7I CTCGAG 1 cut(s) 266
PceI AGGCCT 1 cut(s) 504
PcsI WCGNNNNNNNCGW 2 cut(s) 384, 591
PfoI TCCNGGA 1 cut(s) 697
PinAI ACCGGT 2 cut(s) 212, 441
PkrI GCNGC 2 cut(s) 118, 143
PleI GAGTC 2 cut(s) 104, 377
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 2 cut(s) 104, 377
Psp124BI GAGCTC 1 cut(s) 552
Psp6I CCWGG 1 cut(s) 633
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 633
PspN4I GGNNCC 5 cut(s) 180, 198, 328, 406, 621
PspOMI GGGCCC 1 cut(s) 178
PspPI GGNCC 3 cut(s) 178, 179, 620
PsuI RGATCY 2 cut(s) 262, 514
RsaI GTAC 4 cut(s) 152, 173, 476, 597
RsaNI GTAC 4 cut(s) 151, 172, 475, 596
SacI GAGCTC 1 cut(s) 552
SaqAI TTAA 1 cut(s) 660
SatI GCNGC 2 cut(s) 117, 142
Sau3AI GATC 3 cut(s) 160, 262, 514
Sau96I GGNCC 3 cut(s) 178, 179, 620
ScaI AGTACT 1 cut(s) 173
SchI GAGTC 2 cut(s) 105, 378
ScrFI CCNGG 6 cut(s) 225, 226, 480, 613, 635, 699
SduI GDGCHC 4 cut(s) 182, 409, 451, 552
SfcI CTRYAG 1 cut(s) 431
SfoI GGCGCC 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 266
SgrAI CRCCGGYG 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 266
SmaI CCCGGG 1 cut(s) 226
SmlI CTYRAG 3 cut(s) 266, 293, 551
SmoI CTYRAG 3 cut(s) 266, 293, 551
SseBI AGGCCT 1 cut(s) 504
SsiI CCGC 4 cut(s) 114, 260, 361, 483
SspDI GGCGCC 1 cut(s) 326
SspMI CTAG 3 cut(s) 38, 356, 566
SstI GAGCTC 1 cut(s) 552
StuI AGGCCT 1 cut(s) 504
StyD4I CCNGG 6 cut(s) 223, 224, 478, 611, 633, 697
StyI CCWWGG 3 cut(s) 82, 146, 330
TaaI ACNGT 4 cut(s) 133, 155, 236, 287
TaqI TCGA 2 cut(s) 267, 492
TatI WGTACW 1 cut(s) 171
Tru1I TTAA 1 cut(s) 660
Tru9I TTAA 1 cut(s) 660
TscAI CASTG 2 cut(s) 250, 424
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 116, 141
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 192
TspGWI ACGGA 1 cut(s) 381
TspMI CCCGGG 1 cut(s) 224
TspRI CASTG 2 cut(s) 250, 424
XagI CCTNNNNNAGG 1 cut(s) 86
XhoI CTCGAG 1 cut(s) 266
XmaI CCCGGG 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 3 cut(s) 38, 356, 566
ZrmI AGTACT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.