Prupe.6G001800_v2.0.a1

NusB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0010441
Physical Location & Seq
Forward (+)
256654 .. 259861
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G001800.1

Sequence Viewer

Length: 915 bp
ATGGAGGGAAGCTCGCTTCTCAGTCACAGCTGCTGCTCTTCTCCCACTTTAAAACCCCATCTATCTTTCTCATTAAGTTTCACTCTCTCCTCTTCTTCGTCTTCTTCTTTGCTCTTCGGAAAATCCCACCTTCTGGGTTTAATCCAAAGACCATTGTTGGCCACCGTATCGCTATCCTCCCCTCGAACCTCTCTCCGCACTTCGACCTTCACTCTCGACGAAGCTCTGGAAAAGCCCAACAGTGATTCAAGAGAGATGTTGCCCAAGATTGATAAGAGTGGCAGATTTTGCAGCCCCAGAGCCGCCAGAGAGCTCGCTCTGTCGATTGTTTATGCTGCTTGTTTAGAAGGTTCTGACCCAGTACGGTTATTCGAAAAGCGAATGAATGTCCGTCGAGAACCCGGGTATGAATTCGACAGGGCTTCGTTGCTTGAATATAATCCCATGAGCTTCGGAGGACCCCCGGTCACAGTTGAAACTGTCGAAGAAGCAGATGAGCTTCTGCGGAATGATGAAAAGGAATCTGCAATTGAAGCAGAAGTTCTTGCAGCTCCTCCAAAATTGGTATACAGCAAACTGATTTTGCGATTTACTAGGAAACTTCTGGTTGCAGTCATGGATAAATGGGACAGTCATGTGCTTGTCATTGACAAGGTTGCCCCTCCAAATTGGAAGGATGAGCCAGCTGGCAGAATCTTGGAACTTTGCATCCTTCACTTGGCAATGTCAGAAATAACAGTGCTAGAAACACGACACCCGATTGTCATTAACGAGGCTGTAGATCTTGCTAAACGGTTCTGTGATGGATCTGCACCCCGTGTTATCAATGGTTGTCTTAGAACATTCGTGAAAGGTATTGAAGAAACTGGTGGTTTAACAACGGCCTTGGGGTATAAACAGAAAGTGGTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NusB

Protein Analysis

305

Amino Acids

33.5

Weight (kDa)

6.62

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26370 AT4G26370 AT4G26370
fragaria_vesca FvH4_3g45910 FvH4_3g45910 FvH4_3g45910
malus_domestica MD11G1001800.v1.1
prunus_persica Prupe.6G001800_v2.0.a1
pyrus_communis pycom11g00180
rosa_chinensis RchiOBHm_Chr5g0084001
rosa_laevigata RLG00000037071
rosa_multiflora Rmu_sc0001137.1_g000001 Rmu_ssc0000372.1_g000077
rosa_roxburghii Rroxscaffold_1G00000650
rosa_rugosa Rorug05G0488200 Rorug05G0488300
rosa_samantha Rh5AG540900 Rh5BG566200 Rh5CG588200 Rh5DG574500
rosa_wichuraiana Rw5G050300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 133
AccI GTMKAC 1 cut(s) 567
AciI CCGC 3 cut(s) 196, 303, 505
AclWI GGATC 1 cut(s) 814
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 410
AdeI CACNNNGTG 1 cut(s) 818
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 2 cut(s) 133, 718
AgsI TTSAA 5 cut(s) 249, 434, 476, 533, 860
AhdI GACNNNNNGTC 1 cut(s) 464
AloI GAACNNNNNNTCC 2 cut(s) 693, 725
AluBI AGCT 8 cut(s) 12, 30, 224, 313, 450, 499, 551, 686
AluI AGCT 8 cut(s) 12, 30, 224, 313, 450, 499, 551, 686
Alw21I GWGCWC 1 cut(s) 315
AlwI GGATC 1 cut(s) 814
AlwNI CAGNNNCTG 1 cut(s) 33
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 2 cut(s) 159, 882
ApeKI GCWGC 5 cut(s) 30, 33, 291, 335, 548
ApoI RAATTY 1 cut(s) 410
AspS9I GGNCC 1 cut(s) 458
AsuC2I CCSGG 3 cut(s) 402, 403, 464
AsuII TTCGAA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 1 cut(s) 458
BalI TGGCCA 1 cut(s) 161
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 5 cut(s) 17, 20, 303, 322, 560
BccI CCATC 2 cut(s) 66, 797
BceAI ACGGC 1 cut(s) 897
BcnI CCSGG 3 cut(s) 402, 403, 464
BfaI CTAG 2 cut(s) 594, 743
BfmI CTRYAG 1 cut(s) 777
BglII AGATCT 1 cut(s) 781
BisI GCNGC 6 cut(s) 31, 34, 292, 303, 336, 549
BlsI GCNGC 6 cut(s) 32, 35, 293, 304, 337, 550
Bme1390I CCNGG 3 cut(s) 402, 403, 464
Bme18I GGWCC 1 cut(s) 458
BmeRI GACNNNNNGTC 1 cut(s) 464
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 3 cut(s) 402, 403, 464
BmrI ACTGGG 1 cut(s) 353
BmsI GCATC 1 cut(s) 717
BmuI ACTGGG 1 cut(s) 353
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 372
BpuMI CCSGG 3 cut(s) 402, 403, 464
BsaJI CCNNGG 3 cut(s) 401, 462, 885
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 718
Bse1I ACTGG 2 cut(s) 359, 871
Bse3DI GCAATG 1 cut(s) 729
BseDI CCNNGG 3 cut(s) 401, 462, 885
BseGI GGATG 2 cut(s) 682, 708
BseLI CCNNNNNNNGG 2 cut(s) 133, 718
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 1 cut(s) 34
BseNI ACTGG 2 cut(s) 359, 871
BseRI GAGGAG 2 cut(s) 79, 543
BseXI GCAGC 5 cut(s) 17, 20, 303, 322, 560
BsgI GTGCAG 1 cut(s) 795
BshFI GGCC 2 cut(s) 161, 884
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 2 cut(s) 402, 464
BslFI GGGAC 1 cut(s) 641
BslI CCNNNNNNNGG 2 cut(s) 133, 718
BsmFI GGGAC 1 cut(s) 641
BsnI GGCC 2 cut(s) 161, 884
BsoBI CYCGRG 1 cut(s) 401
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 2 cut(s) 781, 806
BspACI CCGC 3 cut(s) 196, 303, 505
BspANI GGCC 2 cut(s) 161, 884
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 460
BspPI GGATC 1 cut(s) 814
BspQI GCTCTTC 2 cut(s) 43, 119
BspT104I TTCGAA 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 729
BsrI ACTGG 2 cut(s) 359, 871
BssECI CCNNGG 3 cut(s) 401, 462, 885
BssMI GATC 2 cut(s) 781, 806
BssNAI GTATAC 1 cut(s) 568
BssT1I CCWWGG 1 cut(s) 885
Bst1107I GTATAC 1 cut(s) 568
Bst4CI ACNGT 8 cut(s) 166, 242, 366, 472, 481, 632, 739, 795
Bst6I CTCTTC 3 cut(s) 43, 97, 119
BstAPI GCANNNNNTGC 1 cut(s) 288
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 14, 315, 684, 688
BstDEI CTNAG 2 cut(s) 20, 836
BstF5I GGATG 2 cut(s) 682, 708
BstKTI GATC 2 cut(s) 784, 809
BstMBI GATC 2 cut(s) 781, 806
BstMWI GCNNNNNNNGC 2 cut(s) 288, 533
BstSCI CCNGG 3 cut(s) 400, 401, 462
BstSFI CTRYAG 1 cut(s) 777
BstV1I GCAGC 5 cut(s) 17, 20, 303, 322, 560
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 781, 806
BstYI RGATCY 2 cut(s) 781, 806
BstZ17I GTATAC 1 cut(s) 568
BsuRI GGCC 2 cut(s) 161, 884
BtsCI GGATG 2 cut(s) 682, 708
BtsIMutI CAGTG 2 cut(s) 247, 744
Cac8I GCNNGC 4 cut(s) 14, 315, 684, 688
CaiI CAGNNNCTG 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 458
Cfr9I CCCGGG 1 cut(s) 401
Csp6I GTAC 1 cut(s) 362
CviAII CATG 4 cut(s) 445, 616, 635, 912
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 2 cut(s) 20, 836
DpnI GATC 2 cut(s) 783, 808
DpnII GATC 2 cut(s) 781, 806
DraI TTTAAA 1 cut(s) 51
DraIII CACNNNGTG 1 cut(s) 818
DriI GACNNNNNGTC 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 3 cut(s) 43, 97, 119
Eam1105I GACNNNNNGTC 1 cut(s) 464
EarI CTCTTC 3 cut(s) 43, 97, 119
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 885
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 410
EcoT14I CCWWGG 1 cut(s) 885
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 4 cut(s) 448, 619, 638, 915
FaiI YATR 9 cut(s) 333, 408, 438, 446, 568, 617, 636, 894, 913
FaqI GGGAC 1 cut(s) 641
FatI CATG 4 cut(s) 444, 615, 634, 911
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 6 cut(s) 31, 34, 292, 303, 336, 549
FokI GGATG 2 cut(s) 689, 695
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 6 cut(s) 31, 34, 292, 303, 336, 549
FspBI CTAG 2 cut(s) 594, 743
GluI GCNGC 6 cut(s) 31, 34, 292, 303, 336, 549
HaeIII GGCC 2 cut(s) 161, 884
HapII CCGG 2 cut(s) 402, 464
Hin1II CATG 4 cut(s) 448, 619, 638, 915
HinfI GANTC 3 cut(s) 245, 521, 693
HpaII CCGG 2 cut(s) 402, 464
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 4 cut(s) 119, 355, 455, 730
Hpy188III TCNNGA 5 cut(s) 215, 227, 249, 395, 847
Hpy8I GTNNAC 1 cut(s) 568
Hpy99I CGWCG 2 cut(s) 221, 396
HpyAV CCTTC 5 cut(s) 140, 217, 341, 667, 722
HpyCH4III ACNGT 8 cut(s) 166, 242, 366, 472, 481, 632, 739, 795
HpyCH4V TGCA 6 cut(s) 291, 527, 548, 611, 708, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 533
HpyF3I CTNAG 2 cut(s) 20, 836
Hsp92II CATG 4 cut(s) 448, 619, 638, 915
Kzo9I GATC 2 cut(s) 781, 806
LguI GCTCTTC 2 cut(s) 43, 119
LmnI GCTCC 1 cut(s) 556
Lsp1109I GCAGC 5 cut(s) 17, 20, 303, 322, 560
LweI GCATC 1 cut(s) 717
MaeI CTAG 2 cut(s) 594, 743
MaeIII GTNAC 3 cut(s) 23, 466, 907
MalI GATC 2 cut(s) 783, 808
MboI GATC 2 cut(s) 781, 806
MboII GAAGA 8 cut(s) 30, 84, 87, 93, 96, 106, 497, 872
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 2 cut(s) 781, 806
MhlI GDGCHC 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 4 cut(s) 410, 528, 560, 667
MluNI TGGCCA 1 cut(s) 161
MnlI CCTC 8 cut(s) 100, 187, 192, 199, 449, 564, 672, 766
Mox20I TGGCCA 1 cut(s) 161
MscI TGGCCA 1 cut(s) 161
MseI TTAA 5 cut(s) 50, 74, 140, 768, 875
Msp20I TGGCCA 1 cut(s) 161
MspA1I CMGCKG 2 cut(s) 30, 686
MspI CCGG 2 cut(s) 402, 464
MspR9I CCNGG 3 cut(s) 402, 403, 464
MunI CAATTG 1 cut(s) 528
MwoI GCNNNNNNNGC 2 cut(s) 288, 533
NciI CCSGG 3 cut(s) 402, 403, 464
NdeII GATC 2 cut(s) 781, 806
NlaIII CATG 4 cut(s) 448, 619, 638, 915
NlaIV GGNNCC 1 cut(s) 460
NmuCI GTSAC 3 cut(s) 23, 466, 907
NspV TTCGAA 1 cut(s) 372
PciSI GCTCTTC 2 cut(s) 43, 119
PfeI GAWTC 3 cut(s) 245, 521, 693
PflMI CCANNNNNTGG 1 cut(s) 133
PkrI GCNGC 6 cut(s) 32, 35, 293, 304, 337, 550
PpuMI RGGWCCY 1 cut(s) 458
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 458
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 1 cut(s) 458
PspPPI RGGWCCY 1 cut(s) 458
PstNI CAGNNNCTG 1 cut(s) 33
PsuI RGATCY 2 cut(s) 781, 806
PvuII CAGCTG 2 cut(s) 30, 686
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
SacI GAGCTC 1 cut(s) 315
SapI GCTCTTC 2 cut(s) 43, 119
SaqAI TTAA 5 cut(s) 50, 74, 140, 768, 875
SatI GCNGC 6 cut(s) 31, 34, 292, 303, 336, 549
Sau3AI GATC 2 cut(s) 781, 806
Sau96I GGNCC 1 cut(s) 458
ScrFI CCNGG 3 cut(s) 402, 403, 464
SduI GDGCHC 1 cut(s) 315
SfaNI GCATC 1 cut(s) 717
SfcI CTRYAG 1 cut(s) 777
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 1 cut(s) 458
SmaI CCCGGG 1 cut(s) 403
Sse9I AATT 4 cut(s) 410, 528, 560, 667
SsiI CCGC 3 cut(s) 196, 303, 505
SspMI CTAG 2 cut(s) 594, 743
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 3 cut(s) 400, 401, 462
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 8 cut(s) 166, 242, 366, 472, 481, 632, 739, 795
TaqI TCGA 8 cut(s) 184, 203, 216, 323, 372, 394, 414, 483
TasI AATT 4 cut(s) 410, 528, 560, 667
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 3 cut(s) 245, 521, 693
Tru1I TTAA 5 cut(s) 50, 74, 140, 768, 875
Tru9I TTAA 5 cut(s) 50, 74, 140, 768, 875
TscAI CASTG 2 cut(s) 247, 744
TseFI GTSAC 3 cut(s) 23, 466, 907
TseI GCWGC 5 cut(s) 30, 33, 291, 335, 548
Tsp45I GTSAC 3 cut(s) 23, 466, 907
TspDTI ATGAA 3 cut(s) 398, 423, 528
TspGWI ACGGA 1 cut(s) 380
TspMI CCCGGG 1 cut(s) 401
TspRI CASTG 2 cut(s) 247, 744
Van91I CCANNNNNTGG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 458
XapI RAATTY 1 cut(s) 410
XmaI CCCGGG 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 567
XspI CTAG 2 cut(s) 594, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.