MD11G1096300.v1.1

E3 ubiquitin-protein ligase SDIR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005097
Physical Location & Seq
Forward (+)
8014593 .. 8018606
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1096300.v1.1.491

Sequence Viewer

Length: 858 bp
ATGAGTTTTGTTTTCCGAGGGACTAGAGCAGATATTGAAAGTGGCTTTCCAGGACATATTCCCGAACGACCAGCAATGCGTATACATGCAGCTCGACCAGTTAGTATGAATTCACTGGCATTTCTTGTCACGGTTATACTCCTATTCATGATATTAAACTCTCACCAGATGTCTCCGAACTTTCTGCTGTGGCTAGTTGTGGGTGTCTTTTTGATGGCCACAACCCTGAGGATGTTTGCAACTTGTCAGCAACTTCAAGCCCAAGCCCGATCACATGCTGCAGCTGCAAGTGGCATGCTCAGTCATACGGAACTGCGACTGCACATGCCACCTTCAATAGCTTTTGCAACCAGAGGACGATTACAAGGACTAAGACTCCAGCTGGCACTGCTTGACCGTGAGTTTGATGATCTAGATTATGATACTTTGAGGGCTCTGGACTCTGATAATGCCTCAACAGCAACTTCAATGAGTGAGGAGGAGATTAATGCTTTACCCGTCCACAAGCACAAAGTCACTGGCCCAGAGAACGTTGATGGCTCGTCACTGCAACATGCATCATCATCTTCAGCTCCAGTTGAGCTGACAAAGCAAGATGGGAGCATGAAGGAGCAAGTCTCCAAAAGGATGGACGGGAGCATGAAGGATTTAGAAAATGAGCTGACATGCAGCATTTGCTTAGAGCAAGTTAACAGAGGGGACCTAGTTCGTAGCTTGCCATGTTTGCATCAGTTCCATGCTAACTGCATAGATCCTTGGCTCCGGCAGCAAGGCACATGCCCTGTGTGTAAATTTAGAGCTGGGTCAACATGGCAGGAGAGCCGGGAGAGTGGATCGGACGGTTCGGACATGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

286

Amino Acids

31.49

Weight (kDa)

6.11

Isoelectric Point (pI)

60.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 222 - 264 3e-14 Ring finger domain
zf-RING_11 PF17123 223 - 250 1.2e-09 RING-like zinc finger
zf-C3HC4 PF00097 223 - 263 2.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 223 - 263 4e-09 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 240 - 264 6.6e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 82
AclI AACGTT 1 cut(s) 531
AclWI GGATC 2 cut(s) 746, 841
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 2 cut(s) 109, 791
AcuI CTGAAG 1 cut(s) 552
AgsI TTSAA 4 cut(s) 38, 257, 336, 468
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 9 cut(s) 92, 284, 341, 382, 572, 583, 661, 714, 800
AluI AGCT 9 cut(s) 92, 284, 341, 382, 572, 583, 661, 714, 800
Alw26I GTCTC 2 cut(s) 177, 622
AlwI GGATC 2 cut(s) 746, 841
AoxI GGCC 2 cut(s) 216, 520
ApeKI GCWGC 6 cut(s) 89, 278, 281, 284, 669, 766
ApoI RAATTY 2 cut(s) 109, 791
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AseI ATTAAT 1 cut(s) 486
AspS9I GGNCC 2 cut(s) 521, 700
AsuC2I CCSGG 1 cut(s) 824
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 700
AxyI CCTNAGG 1 cut(s) 227
BalI TGGCCA 1 cut(s) 218
BanII GRGCYC 1 cut(s) 436
BbvI GCAGC 6 cut(s) 101, 265, 271, 293, 681, 778
BccI CCATC 4 cut(s) 208, 530, 590, 622
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 51
BcnI CCSGG 1 cut(s) 824
BcoDI GTCTC 2 cut(s) 177, 622
BfaI CTAG 4 cut(s) 24, 194, 413, 704
BfmI CTRYAG 1 cut(s) 279
BisI GCNGC 6 cut(s) 90, 279, 282, 285, 670, 767
BlsI GCNGC 6 cut(s) 91, 280, 283, 286, 671, 768
Bme1390I CCNGG 2 cut(s) 51, 824
Bme18I GGWCC 1 cut(s) 700
BmgT120I GGNCC 2 cut(s) 521, 700
BmiI GGNNCC 2 cut(s) 701, 761
BmrFI CCNGG 2 cut(s) 51, 824
BmsI GCATC 2 cut(s) 566, 736
BplI GAGNNNNNCTC 2 cut(s) 602, 634
BpmI CTGGAG 2 cut(s) 362, 558
BpuMI CCSGG 1 cut(s) 824
BsaJI CCNNGG 2 cut(s) 16, 755
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bse1I ACTGG 4 cut(s) 98, 120, 523, 575
Bse21I CCTNAGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 16, 755
BseGI GGATG 2 cut(s) 237, 633
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 2 cut(s) 218, 313
BseNI ACTGG 4 cut(s) 98, 120, 523, 575
BseRI GAGGAG 2 cut(s) 491, 494
BseXI GCAGC 6 cut(s) 101, 265, 271, 293, 681, 778
BseYI CCCAGC 1 cut(s) 800
BsgI GTGCAG 1 cut(s) 305
BshFI GGCC 2 cut(s) 218, 522
BsiSI CCGG 2 cut(s) 763, 823
BslFI GGGAC 2 cut(s) 34, 713
BsmAI GTCTC 2 cut(s) 177, 622
BsmFI GGGAC 2 cut(s) 34, 713
BsnI GGCC 2 cut(s) 218, 522
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 4 cut(s) 269, 409, 751, 833
BspANI GGCC 2 cut(s) 218, 522
BspCNI CTCAG 2 cut(s) 219, 312
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 701, 761
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 2 cut(s) 746, 841
BsrDI GCAATG 1 cut(s) 81
BsrI ACTGG 4 cut(s) 98, 120, 523, 575
BssECI CCNNGG 2 cut(s) 16, 755
BssMI GATC 4 cut(s) 269, 409, 751, 833
BssNAI GTATAC 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 755
Bst1107I GTATAC 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 133, 398, 842
BstAPI GCANNNNNTGC 1 cut(s) 675
BstC8I GCNNGC 3 cut(s) 296, 384, 716
BstDEI CTNAG 4 cut(s) 227, 299, 371, 679
BstF5I GGATG 2 cut(s) 237, 633
BstKTI GATC 4 cut(s) 272, 412, 754, 836
BstMAI GTCTC 2 cut(s) 177, 622
BstMBI GATC 4 cut(s) 269, 409, 751, 833
BstMWI GCNNNNNNNGC 7 cut(s) 284, 388, 458, 589, 675, 724, 766
BstNI CCWGG 1 cut(s) 51
BstNSI RCATGY 7 cut(s) 89, 278, 298, 328, 557, 669, 780
BstSCI CCNGG 2 cut(s) 49, 822
BstSFI CTRYAG 1 cut(s) 279
BstV1I GCAGC 6 cut(s) 101, 265, 271, 293, 681, 778
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 628
BstYI RGATCY 1 cut(s) 751
BstZ17I GTATAC 1 cut(s) 83
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 2 cut(s) 218, 522
BtsCI GGATG 2 cut(s) 237, 633
BtsI GCAGTG 2 cut(s) 386, 545
BtsIMutI CAGTG 4 cut(s) 113, 386, 516, 545
Cac8I GCNNGC 3 cut(s) 296, 384, 716
CciI TCATGA 1 cut(s) 147
Cfr13I GGNCC 2 cut(s) 521, 700
DdeI CTNAG 4 cut(s) 227, 299, 371, 679
DpnI GATC 4 cut(s) 271, 411, 753, 835
DpnII GATC 4 cut(s) 269, 409, 751, 833
EaeI YGGCCR 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 755
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 700
Eco57I CTGAAG 1 cut(s) 552
Eco81I CCTNAGG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 700
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 755
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 755
FaqI GGGAC 2 cut(s) 34, 713
FblI GTMKAC 1 cut(s) 82
Fnu4HI GCNGC 6 cut(s) 90, 279, 282, 285, 670, 767
FokI GGATG 2 cut(s) 244, 640
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 6 cut(s) 90, 279, 282, 285, 670, 767
FspBI CTAG 4 cut(s) 24, 194, 413, 704
GluI GCNGC 6 cut(s) 90, 279, 282, 285, 670, 767
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 2 cut(s) 362, 558
HaeIII GGCC 2 cut(s) 218, 522
HapII CCGG 2 cut(s) 763, 823
HincII GTYRAC 2 cut(s) 691, 807
HindII GTYRAC 2 cut(s) 691, 807
HinfI GANTC 2 cut(s) 375, 440
HpaI GTTAAC 1 cut(s) 691
HpaII CCGG 2 cut(s) 763, 823
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 4 cut(s) 83, 502, 691, 807
Hpy188I TCNGA 5 cut(s) 17, 177, 445, 838, 847
Hpy188III TCNNGA 4 cut(s) 62, 148, 413, 437
Hpy8I GTNNAC 4 cut(s) 83, 502, 691, 807
HpyAV CCTTC 3 cut(s) 342, 601, 637
HpyCH4III ACNGT 3 cut(s) 133, 398, 842
HpyCH4IV ACGT 1 cut(s) 531
HpyF10VI GCNNNNNNNGC 7 cut(s) 284, 388, 458, 589, 675, 724, 766
HpyF3I CTNAG 4 cut(s) 227, 299, 371, 679
HpySE526I ACGT 1 cut(s) 531
KspAI GTTAAC 1 cut(s) 691
Kzo9I GATC 4 cut(s) 269, 409, 751, 833
LmnI GCTCC 5 cut(s) 577, 600, 610, 636, 765
Lsp1109I GCAGC 6 cut(s) 101, 265, 271, 293, 681, 778
LweI GCATC 2 cut(s) 566, 736
MaeI CTAG 4 cut(s) 24, 194, 413, 704
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 3 cut(s) 127, 514, 543
MalI GATC 4 cut(s) 271, 411, 753, 835
MboI GATC 4 cut(s) 269, 409, 751, 833
MboII GAAGA 1 cut(s) 558
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 218
MluCI AATT 2 cut(s) 109, 791
MluNI TGGCCA 1 cut(s) 218
MlyI GAGTC 2 cut(s) 369, 434
MnlI CCTC 8 cut(s) 11, 222, 347, 423, 463, 469, 472, 689
Mox20I TGGCCA 1 cut(s) 218
Mph1103I ATGCAT 1 cut(s) 559
MscI TGGCCA 1 cut(s) 218
MseI TTAA 3 cut(s) 155, 486, 690
Msp20I TGGCCA 1 cut(s) 218
MspA1I CMGCKG 2 cut(s) 284, 382
MspI CCGG 2 cut(s) 763, 823
MspR9I CCNGG 2 cut(s) 51, 824
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 7 cut(s) 284, 388, 458, 589, 675, 724, 766
NciI CCSGG 1 cut(s) 824
NdeII GATC 4 cut(s) 269, 409, 751, 833
NlaIV GGNNCC 2 cut(s) 701, 761
NmuCI GTSAC 3 cut(s) 127, 514, 543
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 7 cut(s) 89, 278, 298, 328, 557, 669, 780
PaeI GCATGC 1 cut(s) 298
PagI TCATGA 1 cut(s) 147
PfoI TCCNGGA 1 cut(s) 49
PkrI GCNGC 6 cut(s) 91, 280, 283, 286, 671, 768
PleI GAGTC 2 cut(s) 369, 434
PpsI GAGTC 2 cut(s) 369, 434
PpuMI RGGWCCY 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 486
Psp1406I AACGTT 1 cut(s) 531
Psp5II RGGWCCY 1 cut(s) 700
Psp6I CCWGG 1 cut(s) 49
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 701, 761
PspPI GGNCC 2 cut(s) 521, 700
PspPPI RGGWCCY 1 cut(s) 700
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 751
PvuII CAGCTG 2 cut(s) 284, 382
SaqAI TTAA 3 cut(s) 155, 486, 690
SatI GCNGC 6 cut(s) 90, 279, 282, 285, 670, 767
Sau3AI GATC 4 cut(s) 269, 409, 751, 833
Sau96I GGNCC 2 cut(s) 521, 700
SchI GAGTC 2 cut(s) 369, 434
ScrFI CCNGG 2 cut(s) 51, 824
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 2 cut(s) 566, 736
SfcI CTRYAG 1 cut(s) 279
SinI GGWCC 1 cut(s) 700
SphI GCATGC 1 cut(s) 298
Sse9I AATT 2 cut(s) 109, 791
SspMI CTAG 4 cut(s) 24, 194, 413, 704
StyD4I CCNGG 2 cut(s) 49, 822
StyI CCWWGG 1 cut(s) 755
TaaI ACNGT 3 cut(s) 133, 398, 842
TaiI ACGT 1 cut(s) 534
TaqI TCGA 1 cut(s) 94
TasI AATT 2 cut(s) 109, 791
Tru1I TTAA 3 cut(s) 155, 486, 690
Tru9I TTAA 3 cut(s) 155, 486, 690
TscAI CASTG 4 cut(s) 120, 393, 523, 552
TseFI GTSAC 3 cut(s) 127, 514, 543
TseI GCWGC 6 cut(s) 89, 278, 281, 284, 669, 766
Tsp45I GTSAC 3 cut(s) 127, 514, 543
TspDTI ATGAA 4 cut(s) 122, 136, 620, 656
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 4 cut(s) 120, 393, 523, 552
VpaK11BI GGWCC 1 cut(s) 700
VspI ATTAAT 1 cut(s) 486
XapI RAATTY 2 cut(s) 109, 791
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 7 cut(s) 89, 278, 298, 328, 557, 669, 780
XmiI GTMKAC 1 cut(s) 82
XspI CTAG 4 cut(s) 24, 194, 413, 704
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.