Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004457

Physical Location & Seq

Genomic Coordinates Reverse (-)

14410620 .. 14426279

Gene Structure 15660 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1151500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3345 bp

ATGCAGCCGAATCTGTCTCCCTTCGGCAGCGTTTTGGCGAATCCGTTTTTGTTCAATGGCGATTTGAGCGAGGGCATTGAGACTCCTGGGGTTTTGTTCTTGGTCCCCTTTTTTCTGTTCCAAGGTGGTGCAATGGATTTATCAAAGGTCGGCGAGAAGATTCTGAGCTCCGTTAGGTCTGCCAGATCGCTCGGCCTTCTCCCTTCTGCTTCTGATCGACCAGAGGTTCCCGCACGAGCTGCAGCTGCCGCAGCTGTTGCTCGTGCTATTGCTGGACTGCCTCCGCATCAAAGGTTTAGTCTATCATCAAGCTCTGAAGAATTGAGCTCGATATATGGAAGCAAACATCATGGTCAAGAAGTAGAGGAGATAGAGGAAGAATTCTATGAAGAGAATTTTGATCCAGTCAGACATATTTTGGAGCATATTCCATCTGAAGAAAGTGAGCTCGCTTATTTTGAGAAACAGGCCGCTCTTAGATTGGCACAACTGGATAGAGTAGCTGAACGTTTATCCCGCAATGTTATGGAACATCATGAAGTAATGGTGAAGGGGATGCATTTGGTCAGGGAATTGGAGAAAGACTTGAAAATTGCAAATGTCATCTGCATGAATGGAAGGCGGCATCTGACCTCATCAAGGAATGAGGTTTCTAGGGATCTCATCGTAAATTCCAATTCCAAAAAGAAACAAGCTCTTCTGGACATGCTTCCTGTTTTAACTGAGCTTGGTCATGCACTGAAAATGCAAGCAGAGCTCGAGTCTCTGGTTGAGGAAGGAAATTACTGCAAGGCCTTTCAAGTCCTGTCTGAATATCTACAGCTGTTAGATACTTTTTCAGATCTTTCTGCAGTACAAGAGATGAGCCGTGGTGTAGAGGTTTGGCTGGGAAAAACTCTTCAAAAGCTGGATTCCTTGTTGCTAGGAGTGTGCCAGGAGTTCAATGAGGAGGGCTATATAACTGTGGTCGATGCTTATGCACTAATTGGGGATATCTCTGGCCTTGCTGAGAAGATACAAAGTTTCTTTATGCAGGAAGTTCTATCAGAAACCCACTCTATATTGAAGAATATTGTGCAAGAGGAGGATCAGGGATTTCACATGCAAAATAGTCGACTTACCTACAGCGATCTATGCCTTCAGATACCTGAACCAAAGTTTAGGCAGTGTTTGTTAAACACACTAGCTATCCTTTTCAAGTTGATGTGTTCATATCATGAAATCATGGGCTTTCAGCTGGATAATAGGGATTCAGCCGGTAAAACTCCAAGCATGACACAGAAGGAAAGTGACATCTCTCCGACTCCTGGGGGGGTTCAGCAAATTTCACCTCCTTGTTCTTCCCAAAAAGTTAATGGCTCTCTTGCCGAATCTGTAGATATAGTGCCTGGTTCAGCGTACATAGACGAATCTACAACCACCTGCTCTTTGGTTGAACCTGCAGGAAACACAACTTCCACTAATTATCAAAATTTAGTTGATGAGGCAAGTAAGGATGACAGTACAACATCAACAAGTGGATCCCCATGGTATCAATTACGGAAAGATGCCACAGCATTTGTTTCACAAACCCTTCAGAGGGGACGCAAAAATCTTTGGCAACTCACAGCTACTCGTGTATCGGTCTTGCTTTCTTCGGCTTCTGTTTCTTCTGCTAGCATACACCAATTCTTGAAAAACTATGAAGATCTGGGTGTCTTTATCTTGGCTGGGGAAGCATTTTGTGGAATTGAAGCAGCTGACTTCAGGCAGAAGTTGAAGGCAGTATGTGAAAATTATTTTTTAGCTTTTCATCGGCAAAATATACATGCGCTTAAAATGGTGTTGGAAAGAGAAATTTGGTTGATACTGCCACCAGATACAGTACAAGAAATTACATTTCCCGGGCTTGCCGGTGATGGAGCACCTATAATTGTTCCATCTGAGGGTAATTCTAATGCTAGGGTTCTACATTCTGATAAGTCTACAAGAGTTGTTGATACTGGTGCCAAGAAGAGTGGATTCTCTAATTGGCTAAGAAATGGAAACCCCTTTTTGTTAAAGCTGGCACATACATCCAAAGAAGGTCTTAAGTGGAACAGTACAACTGGTGAATCTGATGGTAATTTTAGTGAAAGGCTTGGTGATAAGGTCTCTCCACAAAACAGTGATTCAAGTCATTCTAATGGTGCTAATTTTGTTTCGGAAGAAGAAAATGAAGATCTTCTTGCAGACTTTATTGATGAGGATAGTCAACTCCCAAGTCGAATTTCGAAACCTAGACTGTCAAGAAATCAATCTTTACATTATAATGATGGAGAAATCATAGCTCAGACTGGATCATCTATTTGTCTTCTAAGGTCAATGGATAAATATGCCAGGCTTATGCAGAAATTGGAAATAGTCAACGTTGAGTTCTTCAAGGGAATATGCCAGTTGTTTGAGGTATTTTTTCATTTTGTGTTTGAAACATTTGCTCAGCAAAACAACAATGCAGGTGGAAAAGGGTCACCTGATCCGATTAATTATCGGCTGAAGACAGCCTTGTCCAGAATACAACAAGACTGTGACCAGTGGATAAAGTCCCCGTCATCTTCATCCACATCTCTAAATTCGTCATATACAGACATCACACCTATGAGTCCTCCCAGTAACACACCAGGAACATCCTTTGGTCTCAAGGAAAGATGCGCAGGTGCTGACACTATCACCCTTGTTGCTCGAATATTGCATAGATCTAAAGCTCATCTCAAGACAATGCTACTTCAGAATAATGCTGCTGTGGTTGAAGACTTCTATGCACATCTGGTTGATGCTGTACCATATCTTGTAGAGCACATTCATAGGACAACTGCAAGACAACTGCTCCATATTAATGGTTATGTGGATCGGATAGCCAATGCAAAATGGGAAGTGAAAGAGCTTGGACTAGAGCATAATGGGTATGTTGATTTGTTATTGGGAGAATTCAAGCACTATAAAACAAGGCTTGCTCATGGGGGAATTCGCAAGGAGGTTCAAGACCTCCTCTTAGAATACGGACTTGAAATTGTGGCCCAAACTCTCATAGAAGGACTCTCCCGTGTCAAGAGGTGTACAGATGAAGGGCGGGCTCTCATGTCATTGGATCTACAGGTTTTGATCAATGGCTTGCAGCATTTTGTGGCAATGAACGTGAAGCCCCATTTACAAATAGTTGAAGCCTTCATTAAGGCATACTATCTTCCGGAAACCGAATATGTGCACTGGGCACGTGCTCACCCGGAATACACAAAAAATCAAATTGTTGGGTTGATCAACCTTGTTGCCACTATGAAAAGTTGGAAGAGAAAAACCAGACTGGAAGTGCTAGAAAAGATTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF2451 Vps54_N

Protein Analysis

1115

Amino Acids

124.21

Weight (kDa)

5.67

Isoelectric Point (pI)

44.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps54_N	PF10475	121 - 411	6.5e-97	Vacuolar-sorting protein 54, of GARP complex
Syndetin_C	PF10474	884 - 1114	1.3e-76	Syndetin, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004457)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27900 AT2G27900
fragaria_vesca	FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380 FvH4_3g30380
malus_domestica	MD03G1129700.v1.1 MD11G1151500.v1.1
prunus_persica	Prupe.6G114700_v2.0.a1 Prupe.6G114700_v2.0.a1 Prupe.6G114700_v2.0.a1
pyrus_communis	pycom11g12360
rosa_chinensis	RchiOBHm_Chr5g0056331
rosa_laevigata	RLG00000035088
rosa_multiflora	Rmu_sc0000317.1_g000010
rosa_roxburghii	Rroxscaffold_1G00023990 Rroxscaffold_1G00027590
rosa_rugosa	Rorug05G0298900 Rorug05G0299000 Rorug05G0299000
rosa_samantha	Rh5AG369700 Rh5BG380700 Rh5CG404100 Rh5DG393900
rosa_wichuraiana	Rw0G012620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2289
AarI	CACCTGC	3 cut(s)	1430, 2465, 2663
AasI	GACNNNNNNGTC	1 cut(s)	2524
Acc16I	TGCGCA	1 cut(s)	2671
Acc36I	ACCTGC	4 cut(s)	1430, 1447, 2465, 2663
AccB1I	GGYRCC	1 cut(s)	1985
AccBSI	CCGCTC	1 cut(s)	473
AccI	GTMKAC	2 cut(s)	1114, 1964
AccIII	TCCGGA	1 cut(s)	3205
AciI	CCGC	7 cut(s)	231, 249, 284, 471, 517, 622, 3088
AclI	AACGTT	2 cut(s)	508, 2388
AclWI	GGATC	9 cut(s)	395, 666, 1095, 1515, 1528, 2326, 2489, 2874, 3114
AcuI	CTGAAG	7 cut(s)	336, 456, 1124, 1559, 1729, 2534, 2729
AcvI	CACGTG	1 cut(s)	3233
AfaI	GTAC	7 cut(s)	855, 1400, 1504, 1866, 2083, 2799, 3076
AfiI	CCNNNNNNNGG	5 cut(s)	639, 1307, 1578, 1579, 1925
AflII	CTTAAG	1 cut(s)	2069
AjnI	CCWGG	6 cut(s)	85, 933, 1306, 1387, 2357, 2638
AjuI	GAANNNNNNNTTGG	2 cut(s)	2233, 2265
Alw21I	GWGCWC	8 cut(s)	170, 329, 450, 759, 1906, 2817, 3225, 3238
Alw26I	GTCTC	5 cut(s)	21, 74, 768, 2137, 2660
Alw44I	GTGCAC	1 cut(s)	3221
AlwI	GGATC	9 cut(s)	395, 666, 1095, 1515, 1528, 2326, 2489, 2874, 3114
AlwNI	CAGNNNCTG	1 cut(s)	2678
Ama87I	CYCGRG	2 cut(s)	758, 1883
Aor13HI	TCCGGA	1 cut(s)	3205
AoxI	GGCC	5 cut(s)	193, 468, 792, 1000, 3033
ApaLI	GTGCAC	1 cut(s)	3221
ApeKI	GCWGC	9 cut(s)	4, 27, 239, 242, 245, 251, 1736, 2756, 3133
ArsI	GACNNNNNNTTYG	2 cut(s)	283, 315
AseI	ATTAAT	2 cut(s)	2502, 2853
Asp700I	GAANNNNTTC	1 cut(s)	2202
AspLEI	GCGC	2 cut(s)	1813, 2672
AspS9I	GGNCC	2 cut(s)	103, 3034
AsuC2I	CCSGG	3 cut(s)	1884, 1885, 3242
AsuHPI	GGTGA	8 cut(s)	559, 1320, 1907, 2102, 2135, 2481, 2680, 3230
AsuII	TTCGAA	1 cut(s)	2252
AsuNHI	GCTAGC	1 cut(s)	1655
AvaI	CYCGRG	2 cut(s)	758, 1883
AvaII	GGWCC	1 cut(s)	103
BaeGI	GKGCMC	2 cut(s)	3225, 3232
BaeI	ACNNNNGTAYC	2 cut(s)	1602, 1635
BamHI	GGATCC	1 cut(s)	1520
BanI	GGYRCC	1 cut(s)	1985
BanII	GRGCYC	5 cut(s)	170, 329, 450, 759, 3094
BauI	CACGAG	3 cut(s)	234, 261, 1614
BbrPI	CACGTG	1 cut(s)	3233
BbsI	GAAGAC	3 cut(s)	2324, 2522, 2775
Bbv12I	GWGCWC	8 cut(s)	170, 329, 450, 759, 1906, 2817, 3225, 3238
BbvI	GCAGC	9 cut(s)	16, 39, 226, 232, 254, 263, 1748, 2743, 3145
BccI	CCATC	5 cut(s)	439, 1892, 1927, 2093, 2288
BceAI	ACGGC	1 cut(s)	852
BcgI	CGANNNNNNTGC	4 cut(s)	959, 993, 1094, 1128
BciT130I	CCWGG	6 cut(s)	87, 935, 1308, 1389, 2359, 2640
BclI	TGATCA	2 cut(s)	3120, 3273
BcnI	CCSGG	3 cut(s)	1884, 1885, 3242
BcoDI	GTCTC	5 cut(s)	21, 74, 768, 2137, 2660
BfaI	CTAG	8 cut(s)	654, 923, 1184, 1656, 1941, 2259, 2909, 3329
BfmI	CTRYAG	7 cut(s)	240, 818, 849, 1123, 1374, 1440, 3110
BfrI	CTTAAG	1 cut(s)	2069
BfuAI	ACCTGC	4 cut(s)	1430, 1447, 2465, 2663
BglII	AGATCT	4 cut(s)	841, 1687, 2200, 2714
BlpI	GCTNAGC	1 cut(s)	2457
Bme1390I	CCNGG	9 cut(s)	87, 935, 1308, 1389, 1884, 1885, 2359, 2640, 3242
Bme18I	GGWCC	1 cut(s)	103
BmeT110I	CYCGRG	2 cut(s)	758, 1883
BmgT120I	GGNCC	2 cut(s)	103, 3034
BmiI	GGNNCC	4 cut(s)	105, 228, 1522, 1987
BmrFI	CCNGG	9 cut(s)	87, 935, 1308, 1389, 1884, 1885, 2359, 2640, 3242
BmrI	ACTGGG	2 cut(s)	2622, 3235
BmsI	GCATC	7 cut(s)	295, 546, 634, 961, 1537, 2657, 2782
BmtI	GCTAGC	1 cut(s)	1659
BmuI	ACTGGG	2 cut(s)	2622, 3235
BpiI	GAAGAC	3 cut(s)	2324, 2522, 2775
Bpu1102I	GCTNAGC	1 cut(s)	2457
Bpu14I	TTCGAA	1 cut(s)	2252
BpuEI	CTTGAG	2 cut(s)	2642, 2714
BpuMI	CCSGG	3 cut(s)	1884, 1885, 3242
BsaAI	YACGTR	1 cut(s)	3233
BsaI	GGTCTC	2 cut(s)	2137, 2660
BsaJI	CCNNGG	6 cut(s)	86, 121, 868, 1307, 1526, 1883
BsaWI	WCCGGW	1 cut(s)	3205
BsaXI	ACNNNNNCTCC	2 cut(s)	2829, 2859
Bsc4I	CCNNNNNNNGG	5 cut(s)	639, 1307, 1578, 1579, 1925
Bse118I	RCCGGY	2 cut(s)	1256, 1892
Bse3DI	GCAATG	3 cut(s)	138, 526, 3153
BseAI	TCCGGA	1 cut(s)	3205
BseBI	CCWGG	6 cut(s)	87, 935, 1308, 1389, 2359, 2640
BseDI	CCNNGG	6 cut(s)	86, 121, 868, 1307, 1526, 1883
BseGI	GGATG	5 cut(s)	561, 1501, 2054, 2576, 2645
BseLI	CCNNNNNNNGG	5 cut(s)	639, 1307, 1578, 1579, 1925
BseMI	GCAATG	3 cut(s)	138, 526, 3153
BseMII	CTCAG	6 cut(s)	155, 714, 999, 1914, 2324, 2471
BseRI	GAGGAG	4 cut(s)	380, 962, 1097, 2996
BseSI	GKGCMC	2 cut(s)	3225, 3232
BseXI	GCAGC	9 cut(s)	16, 39, 226, 232, 254, 263, 1748, 2743, 3145
BseYI	CCCAGC	2 cut(s)	886, 1709
BshFI	GGCC	5 cut(s)	195, 470, 794, 1002, 3035
BshNI	GGYRCC	1 cut(s)	1985
BsiHKAI	GWGCWC	8 cut(s)	170, 329, 450, 759, 1906, 2817, 3225, 3238
BsiHKCI	CYCGRG	2 cut(s)	758, 1883
BsiSI	CCGG	5 cut(s)	1257, 1884, 1893, 3206, 3242
BslFI	GGGAC	3 cut(s)	89, 1596, 2548
BslI	CCNNNNNNNGG	5 cut(s)	639, 1307, 1578, 1579, 1925
BsmAI	GTCTC	5 cut(s)	21, 74, 768, 2137, 2660
BsmFI	GGGAC	3 cut(s)	89, 1596, 2548
BsnI	GGCC	5 cut(s)	195, 470, 794, 1002, 3035
Bso31I	GGTCTC	2 cut(s)	2137, 2660
BsoBI	CYCGRG	2 cut(s)	758, 1883
Bsp119I	TTCGAA	1 cut(s)	2252
Bsp13I	TCCGGA	1 cut(s)	3205
Bsp1407I	TGTACA	1 cut(s)	3074
Bsp1720I	GCTNAGC	1 cut(s)	2457
Bsp19I	CCATGG	1 cut(s)	1526
BspACI	CCGC	7 cut(s)	231, 249, 284, 471, 517, 622, 3088
BspANI	GGCC	5 cut(s)	195, 470, 794, 1002, 3035
BspCNI	CTCAG	6 cut(s)	156, 715, 1000, 1915, 2323, 2470
BspEI	TCCGGA	1 cut(s)	3205
BspHI	TCATGA	2 cut(s)	535, 1216
BspLI	GGNNCC	4 cut(s)	105, 228, 1522, 1987
BspMAI	CTGCAG	3 cut(s)	244, 853, 1444
BspMI	ACCTGC	4 cut(s)	1430, 1447, 2465, 2663
BspOI	GCTAGC	1 cut(s)	1659
BspPI	GGATC	9 cut(s)	395, 666, 1095, 1515, 1528, 2326, 2489, 2874, 3114
BspQI	GCTCTTC	1 cut(s)	702
BspT104I	TTCGAA	1 cut(s)	2252
BspT107I	GGYRCC	1 cut(s)	1985
BspTI	CTTAAG	1 cut(s)	2069
BspTNI	GGTCTC	2 cut(s)	2137, 2660
BsrBI	CCGCTC	1 cut(s)	473
BsrDI	GCAATG	3 cut(s)	138, 526, 3153
BsrFI	RCCGGY	2 cut(s)	1256, 1892
BsrGI	TGTACA	1 cut(s)	3074
BssAI	RCCGGY	2 cut(s)	1256, 1892
BssECI	CCNNGG	6 cut(s)	86, 121, 868, 1307, 1526, 1883
BssSI	CACGAG	3 cut(s)	234, 261, 1614
BssT1I	CCWWGG	2 cut(s)	121, 1526
Bst2BI	CACGAG	3 cut(s)	234, 261, 1614
Bst2UI	CCWGG	6 cut(s)	87, 935, 1308, 1389, 2359, 2640
Bst4CI	ACNGT	7 cut(s)	964, 1502, 1864, 2081, 2147, 2265, 2546
Bst6I	CTCTTC	5 cut(s)	384, 702, 903, 1988, 3299
BstAFI	CTTAAG	1 cut(s)	2069
BstAPI	GCANNNNNTGC	2 cut(s)	239, 257
BstAUI	TGTACA	1 cut(s)	3074
BstBAI	YACGTR	1 cut(s)	3233
BstBI	TTCGAA	1 cut(s)	2252
BstC8I	GCNNGC	8 cut(s)	450, 750, 1657, 1890, 2046, 2970, 3090, 3131
BstDSI	CCRYGG	2 cut(s)	868, 1526
BstEII	GGTNACC	1 cut(s)	2487
BstENI	CCTNNNNNAGG	1 cut(s)	637
BstF5I	GGATG	5 cut(s)	561, 1501, 2054, 2576, 2645
BstHHI	GCGC	2 cut(s)	1813, 2672
BstMAI	GTCTC	5 cut(s)	21, 74, 768, 2137, 2660
BstMWI	GCNNNNNNNGC	9 cut(s)	66, 239, 245, 248, 251, 257, 754, 1134, 1715
BstNI	CCWGG	6 cut(s)	87, 935, 1308, 1389, 2359, 2640
BstNSI	RCATGY	3 cut(s)	709, 1105, 1811
BstPI	GGTNACC	1 cut(s)	2487
BstSCI	CCNGG	9 cut(s)	85, 933, 1306, 1387, 1882, 1883, 2357, 2638, 3240
BstSFI	CTRYAG	7 cut(s)	240, 818, 849, 1123, 1374, 1440, 3110
BstSLI	GKGCMC	2 cut(s)	3225, 3232
BstV1I	GCAGC	9 cut(s)	16, 39, 226, 232, 254, 263, 1748, 2743, 3145
BstV2I	GAAGAC	3 cut(s)	2324, 2522, 2775
BstX2I	RGATCY	7 cut(s)	658, 841, 1520, 1687, 2200, 2714, 3106
BstXI	CCANNNNNNTGG	1 cut(s)	2855
BstYI	RGATCY	7 cut(s)	658, 841, 1520, 1687, 2200, 2714, 3106
BsuRI	GGCC	5 cut(s)	195, 470, 794, 1002, 3035
BtgI	CCRYGG	2 cut(s)	868, 1526
BtsCI	GGATG	5 cut(s)	561, 1501, 2054, 2576, 2645
BtsI	GCAGTG	1 cut(s)	1172
BtsIMutI	CAGTG	5 cut(s)	737, 1172, 2152, 2558, 3223
BveI	ACCTGC	4 cut(s)	1430, 1447, 2465, 2663
Cac8I	GCNNGC	8 cut(s)	450, 750, 1657, 1890, 2046, 2970, 3090, 3131
CaiI	CAGNNNCTG	1 cut(s)	2678
CciI	TCATGA	2 cut(s)	535, 1216
CfoI	GCGC	2 cut(s)	1813, 2672
Cfr10I	RCCGGY	2 cut(s)	1256, 1892
Cfr13I	GGNCC	2 cut(s)	103, 3034
Cfr9I	CCCGGG	1 cut(s)	1883
CseI	GACGC	1 cut(s)	1593
Csp6I	GTAC	7 cut(s)	854, 1399, 1503, 1865, 2082, 2798, 3075
CspCI	CAANNNNNGTGG	6 cut(s)	1540, 1575, 1978, 2013, 2458, 2493
CviQI	GTAC	7 cut(s)	854, 1399, 1503, 1865, 2082, 2798, 3075
DrdI	GACNNNNNNGTC	1 cut(s)	2524
DseDI	GACNNNNNNGTC	1 cut(s)	2524
Eam1104I	CTCTTC	5 cut(s)	384, 702, 903, 1988, 3299
EarI	CTCTTC	5 cut(s)	384, 702, 903, 1988, 3299
Ecl136II	GAGCTC	4 cut(s)	168, 327, 448, 757
Eco130I	CCWWGG	2 cut(s)	121, 1526
Eco147I	AGGCCT	1 cut(s)	794
Eco24I	GRGCYC	5 cut(s)	170, 329, 450, 759, 3094
Eco31I	GGTCTC	2 cut(s)	2137, 2660
Eco32I	GATATC	1 cut(s)	994
Eco47I	GGWCC	1 cut(s)	103
Eco53kI	GAGCTC	4 cut(s)	168, 327, 448, 757
Eco57I	CTGAAG	7 cut(s)	336, 456, 1124, 1559, 1729, 2534, 2729
Eco72I	CACGTG	1 cut(s)	3233
Eco88I	CYCGRG	2 cut(s)	758, 1883
Eco91I	GGTNACC	1 cut(s)	2487
EcoICRI	GAGCTC	4 cut(s)	168, 327, 448, 757
EcoNI	CCTNNNNNAGG	1 cut(s)	637
EcoO65I	GGTNACC	1 cut(s)	2487
EcoRI	GAATTC	3 cut(s)	380, 2945, 2982
EcoRII	CCWGG	6 cut(s)	85, 933, 1306, 1387, 2357, 2638
EcoRV	GATATC	1 cut(s)	994
EcoT14I	CCWWGG	2 cut(s)	121, 1526
EcoT22I	ATGCAT	1 cut(s)	561
EcoT38I	GRGCYC	5 cut(s)	170, 329, 450, 759, 3094
ErhI	CCWWGG	2 cut(s)	121, 1526
FalI	AAGNNNNNCTT	6 cut(s)	2052, 2084, 2190, 2222, 2507, 2539
FaqI	GGGAC	3 cut(s)	89, 1596, 2548
FauI	CCCGC	3 cut(s)	238, 524, 3081
FbaI	TGATCA	2 cut(s)	3120, 3273
FblI	GTMKAC	2 cut(s)	1114, 1964
FokI	GGATG	5 cut(s)	568, 1508, 2041, 2563, 2632
FriOI	GRGCYC	5 cut(s)	170, 329, 450, 759, 3094
FspBI	CTAG	8 cut(s)	654, 923, 1184, 1656, 1941, 2259, 2909, 3329
FspI	TGCGCA	1 cut(s)	2671
GlaI	GCGC	2 cut(s)	1812, 2671
GsaI	CCCAGC	2 cut(s)	890, 1713
HaeIII	GGCC	5 cut(s)	195, 470, 794, 1002, 3035
HapII	CCGG	5 cut(s)	1257, 1884, 1893, 3206, 3242
HgaI	GACGC	1 cut(s)	1593
HhaI	GCGC	2 cut(s)	1813, 2672
Hin6I	GCGC	2 cut(s)	1811, 2670
HinP1I	GCGC	2 cut(s)	1811, 2670
HincII	GTYRAC	3 cut(s)	1115, 2234, 2386
HindII	GTYRAC	3 cut(s)	1115, 2234, 2386
HpaII	CCGG	5 cut(s)	1257, 1884, 1893, 3206, 3242
HphI	GGTGA	8 cut(s)	559, 1320, 1907, 2102, 2135, 2481, 2680, 3230
Hpy166II	GTNNAC	6 cut(s)	1115, 1965, 2234, 2386, 3075, 3223
Hpy8I	GTNNAC	6 cut(s)	1115, 1965, 2234, 2386, 3075, 3223
HpyCH4III	ACNGT	7 cut(s)	964, 1502, 1864, 2081, 2147, 2265, 2546
HpyCH4IV	ACGT	4 cut(s)	508, 2388, 3153, 3232
HpyF10VI	GCNNNNNNNGC	9 cut(s)	66, 239, 245, 248, 251, 257, 754, 1134, 1715
HpySE526I	ACGT	4 cut(s)	508, 2388, 3153, 3232
HspAI	GCGC	2 cut(s)	1811, 2670
Kpn2I	TCCGGA	1 cut(s)	3205
Ksp22I	TGATCA	2 cut(s)	3120, 3273
LguI	GCTCTTC	1 cut(s)	702
LmnI	GCTCC	4 cut(s)	173, 421, 1901, 2850
Lsp1109I	GCAGC	9 cut(s)	16, 39, 226, 232, 254, 263, 1748, 2743, 3145
LweI	GCATC	7 cut(s)	295, 546, 634, 961, 1537, 2657, 2782
MaeI	CTAG	8 cut(s)	654, 923, 1184, 1656, 1941, 2259, 2909, 3329
MaeII	ACGT	4 cut(s)	508, 2388, 3153, 3232
MaeIII	GTNAC	4 cut(s)	1289, 2487, 2546, 2630
MbiI	CCGCTC	1 cut(s)	473
MflI	RGATCY	7 cut(s)	658, 841, 1520, 1687, 2200, 2714, 3106
MlyI	GAGTC	5 cut(s)	76, 770, 1297, 2629, 3048
MmeI	TCCRAC	3 cut(s)	1325, 1806, 3281
Mph1103I	ATGCAT	1 cut(s)	561
MroI	TCCGGA	1 cut(s)	3205
MroXI	GAANNNNTTC	1 cut(s)	2202
MseI	TTAA	9 cut(s)	719, 1175, 1353, 1815, 2039, 2070, 2502, 2853, 3189
MslI	CAYNNNNRTG	5 cut(s)	608, 2163, 2289, 2615, 2853
MspA1I	CMGCKG	5 cut(s)	245, 254, 823, 1237, 1739
MspCI	CTTAAG	1 cut(s)	2069
MspI	CCGG	5 cut(s)	1257, 1884, 1893, 3206, 3242
MspR9I	CCNGG	9 cut(s)	87, 935, 1308, 1389, 1884, 1885, 2359, 2640, 3242
MvaI	CCWGG	6 cut(s)	87, 935, 1308, 1389, 2359, 2640
MwoI	GCNNNNNNNGC	9 cut(s)	66, 239, 245, 248, 251, 257, 754, 1134, 1715
NciI	CCSGG	3 cut(s)	1884, 1885, 3242
NcoI	CCATGG	1 cut(s)	1526
NheI	GCTAGC	1 cut(s)	1655
NlaIV	GGNNCC	4 cut(s)	105, 228, 1522, 1987
NmeAIII	GCCGAG	1 cut(s)	171
NmuCI	GTSAC	3 cut(s)	1289, 2487, 2546
NsbI	TGCGCA	1 cut(s)	2671
NsiI	ATGCAT	1 cut(s)	561
NspI	RCATGY	3 cut(s)	709, 1105, 1811
NspV	TTCGAA	1 cut(s)	2252
PaeR7I	CTCGAG	1 cut(s)	758
PagI	TCATGA	2 cut(s)	535, 1216
PaqCI	CACCTGC	3 cut(s)	1430, 2465, 2663
PceI	AGGCCT	1 cut(s)	794
PciSI	GCTCTTC	1 cut(s)	702
PdmI	GAANNNNTTC	1 cut(s)	2202
PleI	GAGTC	5 cut(s)	76, 769, 1297, 2628, 3048
PmaCI	CACGTG	1 cut(s)	3233
PmlI	CACGTG	1 cut(s)	3233
PpsI	GAGTC	5 cut(s)	76, 769, 1297, 2628, 3048
Ppu21I	YACGTR	1 cut(s)	3233
PshBI	ATTAAT	2 cut(s)	2502, 2853
PsiI	TTATAA	1 cut(s)	2289
Psp124BI	GAGCTC	4 cut(s)	170, 329, 450, 759
Psp1406I	AACGTT	2 cut(s)	508, 2388
Psp6I	CCWGG	6 cut(s)	85, 933, 1306, 1387, 2357, 2638
PspCI	CACGTG	1 cut(s)	3233
PspEI	GGTNACC	1 cut(s)	2487
PspFI	CCCAGC	2 cut(s)	886, 1709
PspGI	CCWGG	6 cut(s)	85, 933, 1306, 1387, 2357, 2638
PspN4I	GGNNCC	4 cut(s)	105, 228, 1522, 1987
PspPI	GGNCC	2 cut(s)	103, 3034
PspXI	VCTCGAGB	1 cut(s)	758
PstI	CTGCAG	3 cut(s)	244, 853, 1444
PstNI	CAGNNNCTG	1 cut(s)	2678
PsuI	RGATCY	7 cut(s)	658, 841, 1520, 1687, 2200, 2714, 3106
PvuII	CAGCTG	5 cut(s)	245, 254, 823, 1237, 1739
RsaI	GTAC	7 cut(s)	855, 1400, 1504, 1866, 2083, 2799, 3076
RsaNI	GTAC	7 cut(s)	854, 1399, 1503, 1865, 2082, 2798, 3075
RseI	CAYNNNNRTG	5 cut(s)	608, 2163, 2289, 2615, 2853
SacI	GAGCTC	4 cut(s)	170, 329, 450, 759
SalI	GTCGAC	1 cut(s)	1113
SapI	GCTCTTC	1 cut(s)	702
SaqAI	TTAA	9 cut(s)	719, 1175, 1353, 1815, 2039, 2070, 2502, 2853, 3189
Sau96I	GGNCC	2 cut(s)	103, 3034
SbfI	CCTGCAGG	1 cut(s)	1444
SchI	GAGTC	5 cut(s)	76, 770, 1297, 2629, 3048
ScrFI	CCNGG	9 cut(s)	87, 935, 1308, 1389, 1884, 1885, 2359, 2640, 3242
SdaI	CCTGCAGG	1 cut(s)	1444
SfaNI	GCATC	7 cut(s)	295, 546, 634, 961, 1537, 2657, 2782
SfcI	CTRYAG	7 cut(s)	240, 818, 849, 1123, 1374, 1440, 3110
Sfr274I	CTCGAG	1 cut(s)	758
SfuI	TTCGAA	1 cut(s)	2252
SinI	GGWCC	1 cut(s)	103
SlaI	CTCGAG	1 cut(s)	758
SmaI	CCCGGG	1 cut(s)	1885
SmiMI	CAYNNNNRTG	5 cut(s)	608, 2163, 2289, 2615, 2853
SmlI	CTYRAG	4 cut(s)	758, 2069, 2657, 2729
SmoI	CTYRAG	4 cut(s)	758, 2069, 2657, 2729
Sse8387I	CCTGCAGG	1 cut(s)	1444
SseBI	AGGCCT	1 cut(s)	794
SsiI	CCGC	7 cut(s)	231, 249, 284, 471, 517, 622, 3088
SspI	AATATT	2 cut(s)	1072, 2706
SspMI	CTAG	8 cut(s)	654, 923, 1184, 1656, 1941, 2259, 2909, 3329
SstI	GAGCTC	4 cut(s)	170, 329, 450, 759
StuI	AGGCCT	1 cut(s)	794
StyD4I	CCNGG	9 cut(s)	85, 933, 1306, 1387, 1882, 1883, 2357, 2638, 3240
StyI	CCWWGG	2 cut(s)	121, 1526
TaaI	ACNGT	7 cut(s)	964, 1502, 1864, 2081, 2147, 2265, 2546
TaiI	ACGT	4 cut(s)	511, 2391, 3156, 3235
TaqI	TCGA	8 cut(s)	217, 329, 759, 969, 1114, 2245, 2252, 2701
TaqII	GACCGA	1 cut(s)	1612
TatI	WGTACW	5 cut(s)	853, 1502, 1864, 2081, 3074
TauI	GCSGC	3 cut(s)	251, 473, 625
Tru1I	TTAA	9 cut(s)	719, 1175, 1353, 1815, 2039, 2070, 2502, 2853, 3189
Tru9I	TTAA	9 cut(s)	719, 1175, 1353, 1815, 2039, 2070, 2502, 2853, 3189
TscAI	CASTG	5 cut(s)	744, 1172, 2152, 2558, 3230
TseFI	GTSAC	3 cut(s)	1289, 2487, 2546
TseI	GCWGC	9 cut(s)	4, 27, 239, 242, 245, 251, 1736, 2756, 3133
Tsp45I	GTSAC	3 cut(s)	1289, 2487, 2546
TspGWI	ACGGA	4 cut(s)	33, 160, 1555, 3033
TspMI	CCCGGG	1 cut(s)	1883
TspRI	CASTG	5 cut(s)	744, 1172, 2152, 2558, 3230
Vha464I	CTTAAG	1 cut(s)	2069
VneI	GTGCAC	1 cut(s)	3221
VpaK11BI	GGWCC	1 cut(s)	103
VspI	ATTAAT	2 cut(s)	2502, 2853
XagI	CCTNNNNNAGG	1 cut(s)	637
XceI	RCATGY	3 cut(s)	709, 1105, 1811
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1352, 1426
XhoI	CTCGAG	1 cut(s)	758
XmaI	CCCGGG	1 cut(s)	1883
XmiI	GTMKAC	2 cut(s)	1114, 1964
XmnI	GAANNNNTTC	1 cut(s)	2202
XspI	CTAG	8 cut(s)	654, 923, 1184, 1656, 1941, 2259, 2909, 3329
Zsp2I	ATGCAT	1 cut(s)	561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.