Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004771

Physical Location & Seq

Genomic Coordinates Forward (+)

15344519 .. 15348271

Gene Structure 3753 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1158000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGATCTCACTCACTCCCACTCCCTCTCTCGTCCACCGCACAATCTCCGCTGTCGTCGTCCTCTTCCCGATCCCAAACCCGACAAGGGTGCACCCTAGCAGTAGCACCAGCCCCTTCAGGATCCGATGCTCTTCGGCTGGCTCGGGCTTGCAGGAGGCGCCCAAGTCCTCCGAGGAGAGGAAGCTGAGCTCCGAGCTCTACTCGTCGGTGCCATTCCCGGCGGTGAAGGGGGCAAAGAGGGTGGTGCTGGTGAGGCACGGGCAGAGCACGTGGAACGAGGAAGGCCGGATCCAGGGGAGCTCCAATTTCTCTGTGCTCACCAAGAAAGGCGAGGCTCAGGCTGAGACTTCCCGCCTGATGCTCATCGATGATGCCTTCGATGTCTGCTTCACCAGTCCCCTGACGAGGTCAAAGAGAACAGCTGAAATTATATGGGGCCCTCGCGAAGAGGAAATGATTATGGACTCTGATTTGAGGGAAATTGACTTGTATGCATTCCAAGGCCTCTTGAAGCATGAGGGAAAAGCAAAATTTGGTGAAGCTTTTCATCAATGGCAGGTAGATGCTGCAAACTTTATTATTGATGGTCATTATCCTGTGAGAGAGTTATGGGCGCGTGCTAGGAGCTGCTGGAATCGAATACTGTTGCATGAAAGCAGGTCTGTTCTTGTGGTTGCTCACAATGCTGTTAATCAAGCTCTCATTGCAACTGCTATTGGATTGGGAACGGAGTACTTTAGGGTTTTAGTTCAGAGCAATTGTGGTGTAAGTGTGCTGGATTTCATCCCACGAGCTGAGGGTGGATCACCATTTATTTGCCTCAATCGCCTAAACCAGACCCCAAGTTCACCGGTTGCTAGTGGAAGTTCAGGAGGAAGGAAAGCAAGTAAGCGCATTGTACTTGTTTGTCATGGATCCACACAGAGCGACACAGAGACTGGTTTTCCTTCAACCGGGGATCAACCAATGGACATGCTTGGGGTTATAAAGTCCCAGAAAACTGCAGAGCTACTTCTTGATTTGAAAGTGAGTTCTATAGTTAGCAGTCCGAAGAAAGCTTGTGTTGAAACAGCAATTACCATCTCCAAAGTGCAAGAAGCTGCAGATTGCTTGGGTGTTGATTGTGTGCCGCGCGACGTGGAAATGAAACAGATGGAGAACCTTGATGTTGAAGACATCCTTCAGCGCTCATCTAAGGCAAGACATCTGAACTTGATAACAGAAGGTGGAACAAAGATTCTACCTATTCGGCCTGGTTGGTTGAATGGATACGAGGACAGTGTGATGGGAGATGTGTGGGACCAATCTGAGAAGGCCTGGCAGTATCTCATAAACGAACTAGCTGCTGAAGCCGAACCAGAAAGAGTTGTTGTAGCAGTTGGCCATCCTGCATTGCACATAGCACTGATGGGCCACTGCCTGAATCTGACAAAAGAATGGTTGGGATCATTTCATCTTGATGCAGGAAGCATCAGTGTCATCGACTTTCCTGACGGTCCTAATGGAAGAGGAGTTATTCGGTGCATAAACTATACCGCTCATATGGGGAGATGGGCAACCCCCATCACCAGATCAACACTCGACGACGAAGAGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0047538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

His_Phos_1

Protein Analysis

533

Amino Acids

58.38

Weight (kDa)

6.13

Isoelectric Point (pI)

49.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
His_Phos_1	PF00300	81 - 262	9.2e-39	Histidine phosphatase superfamily (branch 1)
His_Phos_1	PF00300	299 - 499	1.6e-12	Histidine phosphatase superfamily (branch 1)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004771)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22620 AT5G22620 AT5G22620 AT5G22620 AT5G22620 AT5G22620
fragaria_vesca	FvH4_3g24210
malus_domestica	MD11G1158000.v1.1
prunus_persica	Prupe.6G119800_v2.0.a1 Prupe.6G119800_v2.0.a1 Prupe.6G119800_v2.0.a1
pyrus_communis	pycom03g09120 pycom03g09130 pycom11g12830
rosa_chinensis	RchiOBHm_Chr5g0043311
rosa_laevigata	RLG00000034178
rosa_multiflora	Rmu_sc0000060.1_g000001 Rmu_sc0000060.1_g000024 Rmu_sc0017676.1_g000001 Rmu_sc0025644.1_g000001 Rmu_sc0042577.1_g000001
rosa_roxburghii	Rroxscaffold_1G00037510 Rroxscaffold_7G00214910
rosa_rugosa	Rorug05G0205200
rosa_samantha	Rh5AG290700 Rh5AG290900 Rh5BG297300 Rh5CG327100 Rh5DG306900
rosa_wichuraiana	Rw5G026900 Rw5G026970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	986
Acc36I	ACCTGC	2 cut(s)	547, 648
AccB1I	GGYRCC	2 cut(s)	157, 208
AccBSI	CCGCTC	1 cut(s)	1538
AccII	CGCG	4 cut(s)	444, 616, 1132, 1134
AciI	CCGC	6 cut(s)	37, 48, 221, 352, 1130, 1536
AcoI	YGGCCR	1 cut(s)	1381
AcsI	RAATTY	1 cut(s)	530
AcuI	CTGAAG	3 cut(s)	100, 1166, 1368
AcvI	CACGTG	1 cut(s)	270
AcyI	GRCGYC	1 cut(s)	158
AfaI	GTAC	2 cut(s)	734, 900
AfeI	AGCGCT	1 cut(s)	1187
AfiI	CCNNNNNNNGG	5 cut(s)	85, 177, 292, 405, 953
AgeI	ACCGGT	1 cut(s)	850
AgsI	TTSAA	6 cut(s)	511, 951, 1024, 1067, 1172, 1264
AjiI	CACGTC	1 cut(s)	1138
AjnI	CCWGG	3 cut(s)	291, 1252, 1316
Alw21I	GWGCWC	6 cut(s)	93, 191, 198, 269, 302, 318
Alw26I	GTCTC	2 cut(s)	338, 929
Alw44I	GTGCAC	1 cut(s)	89
AlwNI	CAGNNNCTG	1 cut(s)	938
Ama87I	CYCGRG	1 cut(s)	142
Aor51HI	AGCGCT	1 cut(s)	1187
AoxI	GGCC	7 cut(s)	283, 436, 502, 1250, 1314, 1381, 1411
ApaI	GGGCCC	1 cut(s)	440
ApaLI	GTGCAC	1 cut(s)	89
ApeKI	GCWGC	4 cut(s)	566, 627, 1100, 1343
ApoI	RAATTY	1 cut(s)	530
AsiGI	ACCGGT	1 cut(s)	850
Asp700I	GAANNNNTTC	1 cut(s)	543
AspLEI	GCGC	5 cut(s)	160, 616, 894, 1134, 1188
AspS9I	GGNCC	5 cut(s)	436, 437, 1300, 1411, 1496
AsuC2I	CCSGG	2 cut(s)	218, 955
AsuHPI	GGTGA	8 cut(s)	235, 262, 310, 382, 548, 798, 840, 1558
AvaI	CYCGRG	1 cut(s)	142
AvaII	GGWCC	2 cut(s)	1300, 1496
BaeGI	GKGCMC	2 cut(s)	93, 440
BalI	TGGCCA	1 cut(s)	1383
BamHI	GGATCC	3 cut(s)	120, 288, 914
BanI	GGYRCC	2 cut(s)	157, 208
BanII	GRGCYC	4 cut(s)	191, 198, 302, 440
BauI	CACGAG	1 cut(s)	789
BbrPI	CACGTG	1 cut(s)	270
BbsI	GAAGAC	1 cut(s)	1179
Bbv12I	GWGCWC	6 cut(s)	93, 191, 198, 269, 302, 318
BbvCI	CCTCAGC	1 cut(s)	795
BbvI	GCAGC	4 cut(s)	553, 614, 1087, 1330
BccI	CCATC	8 cut(s)	578, 1088, 1147, 1279, 1392, 1402, 1545, 1571
BcgI	CGANNNNNNTGC	4 cut(s)	70, 104, 1325, 1359
BciT130I	CCWGG	3 cut(s)	293, 1254, 1318
BciVI	GTATCC	1 cut(s)	1262
BcnI	CCSGG	2 cut(s)	218, 955
BcoDI	GTCTC	2 cut(s)	338, 929
BfaI	CTAG	4 cut(s)	96, 621, 858, 1340
BfmI	CTRYAG	3 cut(s)	1002, 1035, 1101
BfoI	RGCGCY	2 cut(s)	161, 1189
BfuAI	ACCTGC	2 cut(s)	547, 648
BfuI	GTATCC	1 cut(s)	1262
BisI	GCNGC	5 cut(s)	567, 628, 1101, 1130, 1344
BlpI	GCTNAGC	1 cut(s)	185
BlsI	GCNGC	5 cut(s)	568, 629, 1102, 1131, 1345
BmcAI	AGTACT	1 cut(s)	734
Bme1390I	CCNGG	5 cut(s)	218, 293, 955, 1254, 1318
Bme18I	GGWCC	2 cut(s)	1300, 1496
BmeT110I	CYCGRG	1 cut(s)	142
BmgBI	CACGTC	1 cut(s)	1138
BmgT120I	GGNCC	5 cut(s)	436, 437, 1300, 1411, 1496
BmiI	GGNNCC	8 cut(s)	122, 159, 210, 290, 437, 438, 916, 1301
BmrFI	CCNGG	5 cut(s)	218, 293, 955, 1254, 1318
BmsI	GCATC	6 cut(s)	116, 348, 361, 553, 1450, 1479
BpiI	GAAGAC	1 cut(s)	1179
BplI	GAGNNNNNCTC	2 cut(s)	185, 217
Bpu10I	CCTNAGC	2 cut(s)	336, 795
Bpu1102I	GCTNAGC	1 cut(s)	185
BpuMI	CCSGG	2 cut(s)	218, 955
Bsa29I	ATCGAT	1 cut(s)	366
BsaAI	YACGTR	1 cut(s)	270
BsaBI	GATNNNNATC	1 cut(s)	362
BsaHI	GRCGYC	1 cut(s)	158
BsaJI	CCNNGG	4 cut(s)	171, 292, 499, 954
BsaWI	WCCGGW	1 cut(s)	850
BsaXI	ACNNNNNCTCC	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	5 cut(s)	85, 177, 292, 405, 953
Bse118I	RCCGGY	1 cut(s)	850
Bse1I	ACTGG	2 cut(s)	393, 943
Bse3DI	GCAATG	2 cut(s)	702, 1391
Bse8I	GATNNNNATC	1 cut(s)	362
BseBI	CCWGG	3 cut(s)	293, 1254, 1318
BseCI	ATCGAT	1 cut(s)	366
BseDI	CCNNGG	4 cut(s)	171, 292, 499, 954
BseGI	GGATG	3 cut(s)	783, 1176, 1384
BseJI	GATNNNNATC	1 cut(s)	362
BseLI	CCNNNNNNNGG	5 cut(s)	85, 177, 292, 405, 953
BseMI	GCAATG	2 cut(s)	702, 1391
BseMII	CTCAG	5 cut(s)	176, 333, 350, 786, 1299
BseNI	ACTGG	2 cut(s)	393, 943
BseRI	GAGGAG	2 cut(s)	188, 1524
BseSI	GKGCMC	2 cut(s)	93, 440
BseXI	GCAGC	4 cut(s)	553, 614, 1087, 1330
Bsh1236I	CGCG	4 cut(s)	444, 616, 1132, 1134
BshFI	GGCC	7 cut(s)	285, 438, 504, 1252, 1316, 1383, 1413
BshNI	GGYRCC	2 cut(s)	157, 208
BshTI	ACCGGT	1 cut(s)	850
BshVI	ATCGAT	1 cut(s)	366
BsiHKAI	GWGCWC	6 cut(s)	93, 191, 198, 269, 302, 318
BsiHKCI	CYCGRG	1 cut(s)	142
BsiSI	CCGG	4 cut(s)	218, 286, 851, 954
BslFI	GGGAC	3 cut(s)	381, 976, 1313
BslI	CCNNNNNNNGG	5 cut(s)	85, 177, 292, 405, 953
BsmAI	GTCTC	2 cut(s)	338, 929
BsmFI	GGGAC	3 cut(s)	381, 976, 1313
BsmI	GAATGC	1 cut(s)	494
BsnI	GGCC	7 cut(s)	285, 438, 504, 1252, 1316, 1383, 1413
BsoBI	CYCGRG	1 cut(s)	142
Bsp120I	GGGCCC	1 cut(s)	436
Bsp1286I	GDGCHC	7 cut(s)	93, 191, 198, 269, 302, 318, 440
Bsp143I	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
Bsp1720I	GCTNAGC	1 cut(s)	185
Bsp68I	TCGCGA	1 cut(s)	444
BspACI	CCGC	6 cut(s)	37, 48, 221, 352, 1130, 1536
BspANI	GGCC	7 cut(s)	285, 438, 504, 1252, 1316, 1383, 1413
BspCNI	CTCAG	5 cut(s)	177, 334, 349, 787, 1300
BspDI	ATCGAT	1 cut(s)	366
BspFNI	CGCG	4 cut(s)	444, 616, 1132, 1134
BspLI	GGNNCC	8 cut(s)	122, 159, 210, 290, 437, 438, 916, 1301
BspMAI	CTGCAG	2 cut(s)	1006, 1105
BspMI	ACCTGC	2 cut(s)	547, 648
BspQI	GCTCTTC	1 cut(s)	136
BspT107I	GGYRCC	2 cut(s)	157, 208
BsrBI	CCGCTC	1 cut(s)	1538
BsrDI	GCAATG	2 cut(s)	702, 1391
BsrFI	RCCGGY	1 cut(s)	850
BsrI	ACTGG	2 cut(s)	393, 943
BssAI	RCCGGY	1 cut(s)	850
BssECI	CCNNGG	4 cut(s)	171, 292, 499, 954
BssMI	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
BssNI	GRCGYC	1 cut(s)	158
BssSI	CACGAG	1 cut(s)	789
BssT1I	CCWWGG	1 cut(s)	499
Bst2BI	CACGAG	1 cut(s)	789
Bst2UI	CCWGG	3 cut(s)	293, 1254, 1318
Bst4CI	ACNGT	3 cut(s)	645, 1280, 1496
Bst6I	CTCTTC	5 cut(s)	68, 136, 441, 1501, 1584
BstACI	GRCGYC	1 cut(s)	158
BstBAI	YACGTR	1 cut(s)	270
BstC8I	GCNNGC	3 cut(s)	139, 149, 618
BstDEI	CTNAG	6 cut(s)	185, 336, 342, 795, 1194, 1308
BstF5I	GGATG	3 cut(s)	783, 1176, 1384
BstFNI	CGCG	4 cut(s)	444, 616, 1132, 1134
BstH2I	RGCGCY	2 cut(s)	161, 1189
BstHHI	GCGC	5 cut(s)	160, 616, 894, 1134, 1188
BstKTI	GATC	9 cut(s)	6, 72, 123, 291, 806, 917, 961, 1448, 1574
BstMAI	GTCTC	2 cut(s)	338, 929
BstMBI	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
BstMWI	GCNNNNNNNGC	6 cut(s)	157, 253, 683, 704, 825, 1349
BstNI	CCWGG	3 cut(s)	293, 1254, 1318
BstNSI	RCATGY	1 cut(s)	976
BstSCI	CCNGG	5 cut(s)	216, 291, 953, 1252, 1316
BstSFI	CTRYAG	3 cut(s)	1002, 1035, 1101
BstSLI	GKGCMC	2 cut(s)	93, 440
BstUI	CGCG	4 cut(s)	444, 616, 1132, 1134
BstV1I	GCAGC	4 cut(s)	553, 614, 1087, 1330
BstV2I	GAAGAC	1 cut(s)	1179
BstX2I	RGATCY	3 cut(s)	120, 288, 914
BstYI	RGATCY	3 cut(s)	120, 288, 914
Bsu15I	ATCGAT	1 cut(s)	366
BsuI	GTATCC	1 cut(s)	1262
BsuRI	GGCC	7 cut(s)	285, 438, 504, 1252, 1316, 1383, 1413
BsuTUI	ATCGAT	1 cut(s)	366
BtrI	CACGTC	1 cut(s)	1138
BtsCI	GGATG	3 cut(s)	783, 1176, 1384
BtsI	GCAGTG	1 cut(s)	1414
BtsIMutI	CAGTG	4 cut(s)	1285, 1403, 1414, 1480
BtuMI	TCGCGA	1 cut(s)	444
BveI	ACCTGC	2 cut(s)	547, 648
Cac8I	GCNNGC	3 cut(s)	139, 149, 618
CaiI	CAGNNNCTG	1 cut(s)	938
CfoI	GCGC	5 cut(s)	160, 616, 894, 1134, 1188
Cfr10I	RCCGGY	1 cut(s)	850
Cfr13I	GGNCC	5 cut(s)	436, 437, 1300, 1411, 1496
ClaI	ATCGAT	1 cut(s)	366
Csp6I	GTAC	2 cut(s)	733, 899
CspAI	ACCGGT	1 cut(s)	850
CspCI	CAANNNNNGTGG	2 cut(s)	222, 257
CviAII	CATG	4 cut(s)	515, 650, 911, 973
CviQI	GTAC	2 cut(s)	733, 899
DdeI	CTNAG	6 cut(s)	185, 336, 342, 795, 1194, 1308
DinI	GGCGCC	1 cut(s)	159
DpnI	GATC	9 cut(s)	5, 71, 122, 290, 805, 916, 960, 1447, 1573
DpnII	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
EaeI	YGGCCR	1 cut(s)	1381
Eam1104I	CTCTTC	5 cut(s)	68, 136, 441, 1501, 1584
EarI	CTCTTC	5 cut(s)	68, 136, 441, 1501, 1584
Ecl136II	GAGCTC	3 cut(s)	189, 196, 300
Eco130I	CCWWGG	1 cut(s)	499
Eco147I	AGGCCT	2 cut(s)	504, 1316
Eco24I	GRGCYC	4 cut(s)	191, 198, 302, 440
Eco47I	GGWCC	2 cut(s)	1300, 1496
Eco47III	AGCGCT	1 cut(s)	1187
Eco53kI	GAGCTC	3 cut(s)	189, 196, 300
Eco57I	CTGAAG	3 cut(s)	100, 1166, 1368
Eco72I	CACGTG	1 cut(s)	270
Eco88I	CYCGRG	1 cut(s)	142
EcoICRI	GAGCTC	3 cut(s)	189, 196, 300
EcoO109I	RGGNCCY	2 cut(s)	436, 437
EcoRII	CCWGG	3 cut(s)	291, 1252, 1316
EcoT14I	CCWWGG	1 cut(s)	499
EcoT22I	ATGCAT	1 cut(s)	496
EcoT38I	GRGCYC	4 cut(s)	191, 198, 302, 440
EgeI	GGCGCC	1 cut(s)	159
EheI	GGCGCC	1 cut(s)	159
ErhI	CCWWGG	1 cut(s)	499
FaeI	CATG	4 cut(s)	518, 653, 914, 976
FalI	AAGNNNNNCTT	2 cut(s)	1164, 1196
FaqI	GGGAC	3 cut(s)	381, 976, 1313
FatI	CATG	4 cut(s)	514, 649, 910, 972
FauI	CCCGC	1 cut(s)	359
FauNDI	CATATG	1 cut(s)	1542
Fnu4HI	GCNGC	5 cut(s)	567, 628, 1101, 1130, 1344
FokI	GGATG	3 cut(s)	770, 1163, 1371
FriOI	GRGCYC	4 cut(s)	191, 198, 302, 440
Fsp4HI	GCNGC	5 cut(s)	567, 628, 1101, 1130, 1344
FspBI	CTAG	4 cut(s)	96, 621, 858, 1340
GlaI	GCGC	5 cut(s)	159, 615, 893, 1133, 1187
GluI	GCNGC	5 cut(s)	567, 628, 1101, 1130, 1344
HaeII	RGCGCY	2 cut(s)	161, 1189
HaeIII	GGCC	7 cut(s)	285, 438, 504, 1252, 1316, 1383, 1413
HapII	CCGG	4 cut(s)	218, 286, 851, 954
HhaI	GCGC	5 cut(s)	160, 616, 894, 1134, 1188
Hin1I	GRCGYC	1 cut(s)	158
Hin1II	CATG	4 cut(s)	518, 653, 914, 976
Hin6I	GCGC	5 cut(s)	158, 614, 892, 1132, 1186
HinP1I	GCGC	5 cut(s)	158, 614, 892, 1132, 1186
HindIII	AAGCTT	2 cut(s)	540, 1056
HinfI	GANTC	4 cut(s)	464, 634, 1237, 1423
HpaII	CCGG	4 cut(s)	218, 286, 851, 954
HphI	GGTGA	8 cut(s)	235, 262, 310, 382, 548, 798, 840, 1558
Hpy166II	GTNNAC	3 cut(s)	34, 91, 848
Hpy188I	TCNGA	9 cut(s)	125, 172, 193, 469, 753, 1050, 1209, 1309, 1428
Hpy188III	TCNNGA	8 cut(s)	67, 118, 443, 508, 870, 1016, 1457, 1490
Hpy8I	GTNNAC	3 cut(s)	34, 91, 848
Hpy99I	CGWCG	5 cut(s)	59, 208, 1139, 1586, 1589
HpyAV	CCTTC	9 cut(s)	124, 220, 275, 385, 870, 957, 1190, 1217, 1306
HpyCH4III	ACNGT	3 cut(s)	645, 1280, 1496
HpyCH4IV	ACGT	2 cut(s)	269, 1137
HpyF10VI	GCNNNNNNNGC	6 cut(s)	157, 253, 683, 704, 825, 1349
HpyF3I	CTNAG	6 cut(s)	185, 336, 342, 795, 1194, 1308
HpySE526I	ACGT	2 cut(s)	269, 1137
Hsp92I	GRCGYC	1 cut(s)	158
Hsp92II	CATG	4 cut(s)	518, 653, 914, 976
HspAI	GCGC	5 cut(s)	158, 614, 892, 1132, 1186
KasI	GGCGCC	1 cut(s)	157
Kzo9I	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
LguI	GCTCTTC	1 cut(s)	136
LmnI	GCTCC	4 cut(s)	194, 297, 305, 624
Lsp1109I	GCAGC	4 cut(s)	553, 614, 1087, 1330
LweI	GCATC	6 cut(s)	116, 348, 361, 553, 1450, 1479
MaeI	CTAG	4 cut(s)	96, 621, 858, 1340
MaeII	ACGT	2 cut(s)	269, 1137
MalI	GATC	9 cut(s)	5, 71, 122, 290, 805, 916, 960, 1447, 1573
MbiI	CCGCTC	1 cut(s)	1538
MboI	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
MboII	GAAGA	6 cut(s)	55, 123, 458, 1063, 1184, 1518
MfeI	CAATTG	1 cut(s)	757
MflI	RGATCY	3 cut(s)	120, 288, 914
MhlI	GDGCHC	7 cut(s)	93, 191, 198, 269, 302, 318, 440
MlsI	TGGCCA	1 cut(s)	1383
MluCI	AATT	6 cut(s)	304, 426, 480, 530, 757, 1074
MluNI	TGGCCA	1 cut(s)	1383
Mly113I	GGCGCC	1 cut(s)	158
MlyI	GAGTC	1 cut(s)	458
Mox20I	TGGCCA	1 cut(s)	1383
Mph1103I	ATGCAT	1 cut(s)	496
MroXI	GAANNNNTTC	1 cut(s)	543
MscI	TGGCCA	1 cut(s)	1383
MseI	TTAA	1 cut(s)	690
MslI	CAYNNNNRTG	1 cut(s)	1458
Msp20I	TGGCCA	1 cut(s)	1383
MspA1I	CMGCKG	2 cut(s)	50, 422
MspI	CCGG	4 cut(s)	218, 286, 851, 954
MspR9I	CCNGG	5 cut(s)	218, 293, 955, 1254, 1318
MunI	CAATTG	1 cut(s)	757
Mva1269I	GAATGC	1 cut(s)	494
MvaI	CCWGG	3 cut(s)	293, 1254, 1318
MvnI	CGCG	4 cut(s)	444, 616, 1132, 1134
MwoI	GCNNNNNNNGC	6 cut(s)	157, 253, 683, 704, 825, 1349
NarI	GGCGCC	1 cut(s)	158
NciI	CCSGG	2 cut(s)	218, 955
NdeI	CATATG	1 cut(s)	1542
NdeII	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
NlaIII	CATG	4 cut(s)	518, 653, 914, 976
NlaIV	GGNNCC	8 cut(s)	122, 159, 210, 290, 437, 438, 916, 1301
NruI	TCGCGA	1 cut(s)	444
NsiI	ATGCAT	1 cut(s)	496
NspI	RCATGY	1 cut(s)	976
PceI	AGGCCT	2 cut(s)	504, 1316
PciSI	GCTCTTC	1 cut(s)	136
PctI	GAATGC	1 cut(s)	494
PdmI	GAANNNNTTC	1 cut(s)	543
PfeI	GAWTC	3 cut(s)	634, 1237, 1423
PflFI	GACNNNGTC	1 cut(s)	406
PinAI	ACCGGT	1 cut(s)	850
PkrI	GCNGC	5 cut(s)	568, 629, 1102, 1131, 1345
PleI	GAGTC	1 cut(s)	458
PluTI	GGCGCC	1 cut(s)	161
PmaCI	CACGTG	1 cut(s)	270
PmlI	CACGTG	1 cut(s)	270
PpsI	GAGTC	1 cut(s)	458
Ppu21I	YACGTR	1 cut(s)	270
PsiI	TTATAA	1 cut(s)	986
Psp124BI	GAGCTC	3 cut(s)	191, 198, 302
Psp6I	CCWGG	3 cut(s)	291, 1252, 1316
PspCI	CACGTG	1 cut(s)	270
PspGI	CCWGG	3 cut(s)	291, 1252, 1316
PspN4I	GGNNCC	8 cut(s)	122, 159, 210, 290, 437, 438, 916, 1301
PspOMI	GGGCCC	1 cut(s)	436
PspPI	GGNCC	5 cut(s)	436, 437, 1300, 1411, 1496
PstI	CTGCAG	2 cut(s)	1006, 1105
PstNI	CAGNNNCTG	1 cut(s)	938
PsuI	RGATCY	3 cut(s)	120, 288, 914
PsyI	GACNNNGTC	1 cut(s)	406
PvuII	CAGCTG	1 cut(s)	422
RruI	TCGCGA	1 cut(s)	444
RsaI	GTAC	2 cut(s)	734, 900
RsaNI	GTAC	2 cut(s)	733, 899
RseI	CAYNNNNRTG	1 cut(s)	1458
SacI	GAGCTC	3 cut(s)	191, 198, 302
SapI	GCTCTTC	1 cut(s)	136
SaqAI	TTAA	1 cut(s)	690
SatI	GCNGC	5 cut(s)	567, 628, 1101, 1130, 1344
Sau3AI	GATC	9 cut(s)	3, 69, 120, 288, 803, 914, 958, 1445, 1571
Sau96I	GGNCC	5 cut(s)	436, 437, 1300, 1411, 1496
ScaI	AGTACT	1 cut(s)	734
SchI	GAGTC	1 cut(s)	458
ScrFI	CCNGG	5 cut(s)	218, 293, 955, 1254, 1318
SduI	GDGCHC	7 cut(s)	93, 191, 198, 269, 302, 318, 440
SfaNI	GCATC	6 cut(s)	116, 348, 361, 553, 1450, 1479
SfcI	CTRYAG	3 cut(s)	1002, 1035, 1101
SfoI	GGCGCC	1 cut(s)	159
SinI	GGWCC	2 cut(s)	1300, 1496
SmiMI	CAYNNNNRTG	1 cut(s)	1458
Sse9I	AATT	6 cut(s)	304, 426, 480, 530, 757, 1074
SseBI	AGGCCT	2 cut(s)	504, 1316
SsiI	CCGC	6 cut(s)	37, 48, 221, 352, 1130, 1536
SspDI	GGCGCC	1 cut(s)	157
SspMI	CTAG	4 cut(s)	96, 621, 858, 1340
SstI	GAGCTC	3 cut(s)	191, 198, 302
StuI	AGGCCT	2 cut(s)	504, 1316
StyD4I	CCNGG	5 cut(s)	216, 291, 953, 1252, 1316
StyI	CCWWGG	1 cut(s)	499
TaaI	ACNGT	3 cut(s)	645, 1280, 1496
TaiI	ACGT	2 cut(s)	272, 1140
TaqI	TCGA	5 cut(s)	366, 378, 637, 1482, 1581
TasI	AATT	6 cut(s)	304, 426, 480, 530, 757, 1074
TatI	WGTACW	2 cut(s)	732, 898
TauI	GCSGC	1 cut(s)	1132
TfiI	GAWTC	3 cut(s)	634, 1237, 1423
Tru1I	TTAA	1 cut(s)	690
Tru9I	TTAA	1 cut(s)	690
TscAI	CASTG	4 cut(s)	1285, 1410, 1421, 1480
TseI	GCWGC	4 cut(s)	566, 627, 1100, 1343
TspDTI	ATGAA	5 cut(s)	536, 666, 772, 1160, 1442
TspGWI	ACGGA	1 cut(s)	743
TspRI	CASTG	4 cut(s)	1285, 1410, 1421, 1480
Tth111I	GACNNNGTC	1 cut(s)	406
VneI	GTGCAC	1 cut(s)	89
VpaK11BI	GGWCC	2 cut(s)	1300, 1496
XapI	RAATTY	1 cut(s)	530
XceI	RCATGY	1 cut(s)	976
XmnI	GAANNNNTTC	1 cut(s)	543
XspI	CTAG	4 cut(s)	96, 621, 858, 1340
ZrmI	AGTACT	1 cut(s)	734
Zsp2I	ATGCAT	1 cut(s)	496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.