Rw5G026900

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004771
Physical Location & Seq
Reverse (-)
39216795 .. 39220160
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026900.1

Sequence Viewer

Length: 1170 bp
ATGCTCTTCGGGCCTCCAAGAGGCCAAGGACACAGATCAGCTCCCATCGGACGAGCTCTCCGCCTCGTCGCCGTTCCCGGCCGTCAAGGCGGCGAAGAGGGTGGTTCTGGTGAGGCACGGGCAGAGCACGTGGAACGAGGAAGTAGGATCCAAGCAGCTCCAATTTCTCAGTGCTCACCAACAAAGGCGAGGCTCAGGCCCAGACTTCCCGCCAAATGCTCATCGGAGATGCCTTCGATGTCTGCTTCGCCAGTCCCTTGGCACGGTCAAAGAGAACAGCTGAAATTATCATGGGACACTCGCAAAGAGGAAATGATTATGGATTTTGATTTGAGGGAAATCGACTTGTACTCCTTTCAAGGCCTCTTAAAGCATGAGGGAAAAGAAAAGTTTGGTGCAGCTTTTCAGAAATGGCAGGTAGATGCACCAAATTTTAGCATTGATGATCATTATCCAGTGAGAGAGTTATGGGCACGTGCTAGGAGCTGCTGGACTAGAATACTATTGCATGAAAGCAGGTCTGTTCTTGTGGTTGCTCATAATGCTGTTAATCAGGCTCTCGTTGCAACAGCCATTGGACTGGGAACAGAGTATTTCAGGACTTTAGTTCAGAGCAATTGTGGGGTGGACCACCATATCTGCCTCAATCGTCTAAACCAGACCCCAAGTTCACCTATTGAAGCTGGAAGTTCAGGGGGAAGGAAAACTAGTAAGCGGATCATACTTGTTTGTCATGGATCCACACAAATAAATGCAGAGCCAATGGACATGCTTGGAGTTATAAAGGCCCAGAAAATTGCCGAGCTACTTCTTGATTTAAAAGTGAACTCTATAGTTAGCAGTCCTAAGGAAGCTTGTGTTGAAACAGCGATTGCCATCTCCAGAGTACAAGAAGCTGCAGATTGTTTGGGAGCTGACTCTGTGCCACGCTCTGTGGAGATGAAGCAGATGGAGAACCTTGATGTTGAAAACATTCTTCAGAAGTCAAATAAGAATGAAATGGGGATTCCATCTCTTCAACCCGGTTGGCTTAATGGGTTTGAGGATAGAGTCGTGGAAGCTGTGTGGGATCAATCTGAGAAGGCCTGGCAGTCTCTGCTAGAAGAAGTAGCTGATGAAGCAGCACCGGAAAAAATGTTGTTGCAGTTGGTCATCCTGCATTGCACATAG

Protein Analysis

389

Amino Acids

42.99

Weight (kDa)

6.44

Isoelectric Point (pI)

57.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 100 - 209 2.8e-14 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
Acc36I ACCTGC 2 cut(s) 406, 507
AciI CCGC 4 cut(s) 61, 90, 210, 715
AclWI GGATC 6 cut(s) 142, 155, 725, 732, 745, 1077
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 962
AcvI CACGTG 2 cut(s) 130, 476
AfaI GTAC 2 cut(s) 350, 888
AfiI CCNNNNNNNGG 2 cut(s) 20, 263
AgsI TTSAA 5 cut(s) 359, 680, 863, 968, 1019
AhlI ACTAGT 1 cut(s) 707
AjnI CCWGG 1 cut(s) 1085
Alw21I GWGCWC 3 cut(s) 58, 129, 176
Alw26I GTCTC 1 cut(s) 1098
AlwI GGATC 6 cut(s) 142, 155, 725, 732, 745, 1077
AlwNI CAGNNNCTG 1 cut(s) 1096
AoxI GGCC 7 cut(s) 11, 22, 79, 197, 361, 786, 1083
ApeKI GCWGC 5 cut(s) 155, 398, 486, 896, 1121
ApoI RAATTY 1 cut(s) 430
Asp700I GAANNNNTTC 1 cut(s) 972
AspS9I GGNCC 4 cut(s) 11, 198, 628, 787
AsuC2I CCSGG 2 cut(s) 78, 1023
AsuHPI GGTGA 3 cut(s) 122, 168, 663
AvaII GGWCC 1 cut(s) 628
AxyI CCTNAGG 1 cut(s) 846
BaeGI GKGCMC 1 cut(s) 475
BamHI GGATCC 2 cut(s) 147, 737
BanII GRGCYC 1 cut(s) 58
BbrPI CACGTG 2 cut(s) 130, 476
Bbv12I GWGCWC 3 cut(s) 58, 129, 176
BbvI GCAGC 5 cut(s) 167, 410, 473, 883, 1133
BccI CCATC 4 cut(s) 53, 884, 943, 1018
BceAI ACGGC 2 cut(s) 56, 66
BciT130I CCWGG 1 cut(s) 1087
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 2 cut(s) 78, 1023
BcoDI GTCTC 1 cut(s) 1098
BcuI ACTAGT 1 cut(s) 707
BfaI CTAG 4 cut(s) 480, 495, 708, 1100
BfmI CTRYAG 2 cut(s) 831, 897
BfuAI ACCTGC 2 cut(s) 406, 507
BglI GCCNNNNNGGC 1 cut(s) 87
BisI GCNGC 6 cut(s) 91, 156, 399, 487, 897, 1122
BlsI GCNGC 6 cut(s) 92, 157, 400, 488, 898, 1123
Bme1390I CCNGG 3 cut(s) 78, 1023, 1087
Bme18I GGWCC 1 cut(s) 628
BmgT120I GGNCC 4 cut(s) 11, 198, 628, 787
BmiI GGNNCC 2 cut(s) 149, 739
BmrFI CCNGG 3 cut(s) 78, 1023, 1087
BmrI ACTGGG 1 cut(s) 590
BmsI GCATC 2 cut(s) 219, 412
BmuI ACTGGG 1 cut(s) 590
BpmI CTGGAG 1 cut(s) 865
Bpu10I CCTNAGC 1 cut(s) 194
BpuMI CCSGG 2 cut(s) 78, 1023
BsaAI YACGTR 2 cut(s) 130, 476
BsaBI GATNNNNATC 2 cut(s) 450, 875
BsaJI CCNNGG 2 cut(s) 25, 257
BsaWI WCCGGW 1 cut(s) 1126
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 263
Bse1I ACTGG 3 cut(s) 251, 455, 585
Bse21I CCTNAGG 1 cut(s) 846
Bse3DI GCAATG 1 cut(s) 1159
Bse8I GATNNNNATC 2 cut(s) 450, 875
BseBI CCWGG 1 cut(s) 1087
BseDI CCNNGG 2 cut(s) 25, 257
BseGI GGATG 1 cut(s) 1152
BseJI GATNNNNATC 2 cut(s) 450, 875
BseLI CCNNNNNNNGG 2 cut(s) 20, 263
BseMI GCAATG 1 cut(s) 1159
BseMII CTCAG 3 cut(s) 182, 208, 1068
BseNI ACTGG 3 cut(s) 251, 455, 585
BseSI GKGCMC 1 cut(s) 475
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 5 cut(s) 167, 410, 473, 883, 1133
BsgI GTGCAG 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 7 cut(s) 13, 24, 81, 199, 363, 788, 1085
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 3 cut(s) 58, 129, 176
BsiSI CCGG 3 cut(s) 78, 1023, 1127
BslFI GGGAC 2 cut(s) 239, 308
BslI CCNNNNNNNGG 2 cut(s) 20, 263
BsmAI GTCTC 1 cut(s) 1098
BsmFI GGGAC 2 cut(s) 239, 308
BsnI GGCC 7 cut(s) 13, 24, 81, 199, 363, 788, 1085
Bsp1286I GDGCHC 4 cut(s) 58, 129, 176, 475
Bsp143I GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
BspACI CCGC 4 cut(s) 61, 90, 210, 715
BspANI GGCC 7 cut(s) 13, 24, 81, 199, 363, 788, 1085
BspCNI CTCAG 3 cut(s) 181, 207, 1069
BspLI GGNNCC 2 cut(s) 149, 739
BspMAI CTGCAG 1 cut(s) 901
BspMI ACCTGC 2 cut(s) 406, 507
BspPI GGATC 6 cut(s) 142, 155, 725, 732, 745, 1077
BspQI GCTCTTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 1159
BsrI ACTGG 3 cut(s) 251, 455, 585
BssECI CCNNGG 2 cut(s) 25, 257
BssMI GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
BssT1I CCWWGG 2 cut(s) 25, 257
Bst2UI CCWGG 1 cut(s) 1087
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 3 cut(s) 11, 90, 1020
BstAPI GCANNNNNTGC 1 cut(s) 1096
BstBAI YACGTR 2 cut(s) 130, 476
BstDEI CTNAG 4 cut(s) 168, 194, 846, 1077
BstENI CCTNNNNNAGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 1152
BstKTI GATC 6 cut(s) 38, 150, 448, 720, 740, 1072
BstMAI GTCTC 1 cut(s) 1098
BstMBI GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 6 cut(s) 10, 87, 542, 563, 1096, 1118
BstNI CCWGG 1 cut(s) 1087
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 3 cut(s) 76, 1021, 1085
BstSFI CTRYAG 2 cut(s) 831, 897
BstSLI GKGCMC 1 cut(s) 475
BstV1I GCAGC 5 cut(s) 167, 410, 473, 883, 1133
BstX2I RGATCY 2 cut(s) 147, 737
BstXI CCANNNNNNTGG 2 cut(s) 258, 580
BstYI RGATCY 2 cut(s) 147, 737
BstZI CGGCCG 1 cut(s) 79
Bsu36I CCTNAGG 1 cut(s) 846
BsuRI GGCC 7 cut(s) 13, 24, 81, 199, 363, 788, 1085
BtsCI GGATG 1 cut(s) 1152
BtsIMutI CAGTG 2 cut(s) 176, 462
BveI ACCTGC 2 cut(s) 406, 507
CaiI CAGNNNCTG 1 cut(s) 1096
Cfr13I GGNCC 4 cut(s) 11, 198, 628, 787
Csp6I GTAC 2 cut(s) 349, 887
CviAII CATG 5 cut(s) 291, 374, 509, 734, 769
CviQI GTAC 2 cut(s) 349, 887
DdeI CTNAG 4 cut(s) 168, 194, 846, 1077
DpnI GATC 6 cut(s) 37, 149, 447, 719, 739, 1071
DpnII GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
DraI TTTAAA 1 cut(s) 819
EaeI YGGCCR 1 cut(s) 79
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 3 cut(s) 11, 90, 1020
EarI CTCTTC 3 cut(s) 11, 90, 1020
EciI GGCGGA 1 cut(s) 50
Ecl136II GAGCTC 1 cut(s) 56
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 25, 257
Eco147I AGGCCT 2 cut(s) 363, 1085
Eco24I GRGCYC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 628
Eco52I CGGCCG 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 962
Eco72I CACGTG 2 cut(s) 130, 476
Eco81I CCTNAGG 1 cut(s) 846
EcoICRI GAGCTC 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 1085
EcoT14I CCWWGG 2 cut(s) 25, 257
EcoT38I GRGCYC 1 cut(s) 58
ErhI CCWWGG 2 cut(s) 25, 257
FaeI CATG 5 cut(s) 294, 377, 512, 737, 772
FaqI GGGAC 2 cut(s) 239, 308
FatI CATG 5 cut(s) 290, 373, 508, 733, 768
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 6 cut(s) 91, 156, 399, 487, 897, 1122
FokI GGATG 1 cut(s) 1139
FriOI GRGCYC 1 cut(s) 58
Fsp4HI GCNGC 6 cut(s) 91, 156, 399, 487, 897, 1122
FspBI CTAG 4 cut(s) 480, 495, 708, 1100
GluI GCNGC 6 cut(s) 91, 156, 399, 487, 897, 1122
GsuI CTGGAG 1 cut(s) 865
HaeIII GGCC 7 cut(s) 13, 24, 81, 199, 363, 788, 1085
HapII CCGG 3 cut(s) 78, 1023, 1127
Hin1II CATG 5 cut(s) 294, 377, 512, 737, 772
HindIII AAGCTT 1 cut(s) 852
HinfI GANTC 3 cut(s) 917, 1006, 1050
HpaII CCGG 3 cut(s) 78, 1023, 1127
HphI GGTGA 3 cut(s) 122, 168, 663
Hpy166II GTNNAC 3 cut(s) 628, 671, 826
Hpy188I TCNGA 6 cut(s) 50, 226, 408, 612, 981, 1078
Hpy188III TCNNGA 3 cut(s) 598, 812, 882
Hpy8I GTNNAC 3 cut(s) 628, 671, 826
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 3 cut(s) 243, 693, 1075
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 2 cut(s) 129, 475
HpyCH4V TGCA 9 cut(s) 398, 425, 508, 566, 755, 899, 1144, 1159, 1164
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 87, 542, 563, 1096, 1118
HpyF3I CTNAG 4 cut(s) 168, 194, 846, 1077
HpySE526I ACGT 2 cut(s) 129, 475
Hsp92II CATG 5 cut(s) 294, 377, 512, 737, 772
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 4 cut(s) 46, 163, 483, 911
Lsp1109I GCAGC 5 cut(s) 167, 410, 473, 883, 1133
LweI GCATC 2 cut(s) 219, 412
MaeI CTAG 4 cut(s) 480, 495, 708, 1100
MaeII ACGT 2 cut(s) 129, 475
MalI GATC 6 cut(s) 37, 149, 447, 719, 739, 1071
MboI GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
MboII GAAGA 4 cut(s) 107, 968, 1007, 1115
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 2 cut(s) 147, 737
MhlI GDGCHC 4 cut(s) 58, 129, 176, 475
MluCI AATT 5 cut(s) 162, 284, 430, 616, 795
MlyI GAGTC 2 cut(s) 911, 1059
MroXI GAANNNNTTC 1 cut(s) 972
MseI TTAA 4 cut(s) 368, 549, 818, 1032
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 3 cut(s) 78, 1023, 1127
MspR9I CCNGG 3 cut(s) 78, 1023, 1087
MunI CAATTG 1 cut(s) 616
MvaI CCWGG 1 cut(s) 1087
MwoI GCNNNNNNNGC 6 cut(s) 10, 87, 542, 563, 1096, 1118
NciI CCSGG 2 cut(s) 78, 1023
NdeII GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
NlaIII CATG 5 cut(s) 294, 377, 512, 737, 772
NlaIV GGNNCC 2 cut(s) 149, 739
NmeAIII GCCGAG 1 cut(s) 826
NspI RCATGY 1 cut(s) 772
PceI AGGCCT 2 cut(s) 363, 1085
PciSI GCTCTTC 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 1 cut(s) 1006
PkrI GCNGC 6 cut(s) 92, 157, 400, 488, 898, 1123
PleI GAGTC 2 cut(s) 911, 1058
PmaCI CACGTG 2 cut(s) 130, 476
PmlI CACGTG 2 cut(s) 130, 476
PpsI GAGTC 2 cut(s) 911, 1058
Ppu21I YACGTR 2 cut(s) 130, 476
PsiI TTATAA 1 cut(s) 782
Psp124BI GAGCTC 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 1085
PspCI CACGTG 2 cut(s) 130, 476
PspGI CCWGG 1 cut(s) 1085
PspN4I GGNNCC 2 cut(s) 149, 739
PspPI GGNCC 4 cut(s) 11, 198, 628, 787
PstI CTGCAG 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 1096
PsuI RGATCY 2 cut(s) 147, 737
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 2 cut(s) 350, 888
RsaNI GTAC 2 cut(s) 349, 887
SacI GAGCTC 1 cut(s) 58
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 4 cut(s) 368, 549, 818, 1032
SatI GCNGC 6 cut(s) 91, 156, 399, 487, 897, 1122
Sau3AI GATC 6 cut(s) 35, 147, 445, 717, 737, 1069
Sau96I GGNCC 4 cut(s) 11, 198, 628, 787
SchI GAGTC 2 cut(s) 911, 1059
ScrFI CCNGG 3 cut(s) 78, 1023, 1087
SduI GDGCHC 4 cut(s) 58, 129, 176, 475
SfaNI GCATC 2 cut(s) 219, 412
SfcI CTRYAG 2 cut(s) 831, 897
SinI GGWCC 1 cut(s) 628
SpeI ACTAGT 1 cut(s) 707
Sse9I AATT 5 cut(s) 162, 284, 430, 616, 795
SseBI AGGCCT 2 cut(s) 363, 1085
SsiI CCGC 4 cut(s) 61, 90, 210, 715
SspMI CTAG 4 cut(s) 480, 495, 708, 1100
SstI GAGCTC 1 cut(s) 58
StuI AGGCCT 2 cut(s) 363, 1085
StyD4I CCNGG 3 cut(s) 76, 1021, 1085
StyI CCWWGG 2 cut(s) 25, 257
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 2 cut(s) 132, 478
TaqI TCGA 2 cut(s) 236, 342
TasI AATT 5 cut(s) 162, 284, 430, 616, 795
TatI WGTACW 2 cut(s) 348, 886
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 1 cut(s) 1006
Tru1I TTAA 4 cut(s) 368, 549, 818, 1032
Tru9I TTAA 4 cut(s) 368, 549, 818, 1032
TscAI CASTG 2 cut(s) 176, 462
TseI GCWGC 5 cut(s) 155, 398, 486, 896, 1121
TspDTI ATGAA 4 cut(s) 525, 956, 1011, 1131
TspRI CASTG 2 cut(s) 176, 462
VpaK11BI GGWCC 1 cut(s) 628
XagI CCTNNNNNAGG 1 cut(s) 18
XapI RAATTY 1 cut(s) 430
XceI RCATGY 1 cut(s) 772
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 4 cut(s) 480, 495, 708, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.