Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0011058

Physical Location & Seq

Genomic Coordinates Reverse (-)

27247255 .. 27251170

Gene Structure 3916 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1192000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGACGACGACGACGACGTTCGGAGCTACGAACACGGCGGTGCTGGGGGAGCCCAAGGTGCAGATTGGGAGGTACCAGGGGCTGCGCTCAGCAAACTCGCTTTGCCTCACCAGAAGCCGACAAGTGCCTCTCTCTTCCGTTTCTTCCTCTTCGCTTATTCGAGCCGTCGCTACGCCTCCAAAGGCCGAGACTGCAACTGAGACGAAGCGGAGTAAGGTTGAAATATTCAAAGAACAGAGCAACTTCATCAGATACCCTCTTAATGAAGAGATTTTGACGGATGCCCCGAACATTAATGAGGCTGCTACGCAACTCATTAAGTTCCACGGGAGCTATCAACAATATAACCGAGATGAACGTGGTGGAAGGTCCTACTCCTTCATGCTTCGTACCAAGAACCCTTGTGGGAAAGTGTCAAACCAATTATACTTGACCATGGATGATCTTGCTGATCAGTTTGGAATTGGTACTCTTCGTTTGACCACAAGGCAGACGTTTCAGCTCCATGGGGTTCTGAAAAAGGATCTAAAGACTGTAATGAGTAGCATCATTAGAAGCATGGGTTCAACTCTTGGTGCCTGTGGTGATCTTAACAGAAACGTTCTTGCTCCTCCTGCTCCAATACAAAGAAAGGATTACCTTTGCGCACAGCAGACTGCTGAGAACATTGCAGCATTGCTGACTCCACAGTCTGGATTTTACTACGATGTTTGGGTGGATGGAGAAAAGTTCCTGACTGCAGAATCTCCTGAAGTAACAAAGGCCCGCAATGATAATTCTCATGGAACAAACTTCCCTGATTCACCTGAGCCCATTTATGGAACTCAGTTCTTGCCCAGGAAGTTTAAAGTTGCAGTTACAGTGCCAACAGACAACTCGGTGGATATTCTCACAAATGATATTGGTGTTGTGGTTGTTTCTGATGATAACGGGGAGCCTCAAGGGTTTAACCTATATGTTGGTGGTGGTATGGGAAGGACACACAGGATGGAATCCACTTTTCCCCGTCTGGCTGAGCCATTGGGTTATGTCCCCAAAGAGGATATCCTATATGCCATTAAGGCTATAGTTGTTACACAACGTGAAAATGGGAGAAGAGATGATCGTAGGTATAGTAGGATGAAATATTTGATCAGCTCGTGGGGGATTGAAAAGTTCAGAAGTGTAGTGGAACAATACTACGGAAAGAAATTTGAGCCTTTCCGTGAGTTGCCGGAGTGGGAATTCAAAAGTCATTTGGGATGGCACAAGCAGGGTGATGGCAGTTATTATTGTGGGCTTCATGTTGATAATGGTCGGATTGGAGGAGTTATGAAGCAGGCATTAAGAGAGGTTATAGAAAAGTATAATTTGAGTATAAGGCTCACACCAAACCAGAATATCATATTGTGTGATATTCGCAGTGCATGGAAGCGTCCCATCACCACAGTTCTTGCAAAGGCCGGTCTGCTGCATCCTAGGTTTGTAGACCCCCTCAACCTAACTGCCATGGCATGCCCAGCCTTCCCACTATGCCCACTGGCAATAACTGAAGCTGAACGAGGTATACCTGACATTCTTAAGCGGGTTCGAGCAGTATTTGAGAAGGTTGGTCTGAAGTACAATGAATCAGTAGTTATAAGGATTACTGGATGCCCTAATGGGTGTGCTAGGCCATACATGGCTGAACTTGGACTAGTTGGTGATGGTCCAAATAGCTATCAGATCTGGCTTGGAGGAACATCCAATCAAACCTCACTCGCAAAATCCTTCATGAACAAGGTCAAGGTTCAAGACCTTGAAAAGGTTTTCGAACCTTTGTTCTATTACTGGAAACGTAAACGACAATCTAAAGAGTCATTTGGCAACTTCACAAACCGCTTGGGATTTGAGAAACTTCAAGAGTTGGTTGACAAATGGGAAGGTCCAGAGGCATCACCAACACGTTACAACTTGAAACTTTTTGCTGATAAGGAAACGTATGAAGCTGTGGATGAACTTGCAAAGCTGCAGAATAAGAATGCTCACCAGTTAGCAATGGAAGTTATTCGCAATTTTGTTGCTTCCCAGCACAATGGGAAAAGCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006275 GO:0006323 GO:0006355 GO:0006790 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0008152 GO:0009266 GO:0009295 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009889 GO:0009890 GO:0009892 GO:0009941 GO:0009987 GO:0010319 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016002 GO:0016020 GO:0016043 GO:0016491 GO:0016667 GO:0016673 GO:0019219 GO:0019222 GO:0019418 GO:0019419 GO:0019424 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031967 GO:0031975 GO:0036385 GO:0042644 GO:0042646 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0046872 GO:0046914 GO:0048046 GO:0048519 GO:0048523 GO:0050311 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0055114 GO:0060255 GO:0065007 GO:0071103 GO:0071840 GO:0080090 GO:0097159 GO:1900160 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko00920 ko01100 ko01120 map00920 map01100 map01120

Pfam Domains

Protein Families

NIR_SIR NIR_SIR_ferr

Protein Analysis

690

Amino Acids

77.56

Weight (kDa)

9.2

Isoelectric Point (pI)

42.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIR_SIR_ferr	PF03460	126 - 181	1.5e-14	Nitrite/Sulfite reductase ferredoxin-like half domain
NIR_SIR	PF01077	223 - 398	2.5e-42	Nitrite and sulphite reductase 4Fe-4S domain
NIR_SIR_ferr	PF03460	416 - 468	1.6e-12	Nitrite/Sulfite reductase ferredoxin-like half domain
NIR_SIR	PF01077	495 - 632	5.2e-11	Nitrite and sulphite reductase 4Fe-4S domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04590
fragaria_vesca	FvH4_3g28720 FvH4_3g28720 FvH4_3g28720 FvH4_3g28720
malus_domestica	MD03G1173800.v1.1 MD11G1192000.v1.1
prunus_persica	Prupe.4G250500_v2.0.a1
pyrus_communis	pycom03g13140 pycom11g16770
rosa_chinensis	RchiOBHm_Chr5g0052341
rosa_laevigata	RLG00000034823
rosa_multiflora	Rmu_sc0000968.1_g000009
rosa_roxburghii	Rroxscaffold_1G00028250
rosa_rugosa	Rorug05G0272600
rosa_samantha	Rh5AG345200 Rh5BG356300 Rh5CG381600 Rh5DG371400
rosa_wichuraiana	Rw5G032580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1619
AasI	GACNNNNNNGTC	1 cut(s)	688
Acc16I	TGCGCA	1 cut(s)	646
Acc65I	GGTACC	1 cut(s)	72
AccB1I	GGYRCC	2 cut(s)	72, 575
AccB7I	CCANNNNNTGG	1 cut(s)	692
AccI	GTMKAC	2 cut(s)	1467, 1546
AciI	CCGC	5 cut(s)	38, 208, 766, 1564, 1858
AclI	AACGTT	1 cut(s)	600
AclWI	GGATC	1 cut(s)	531
AcsI	RAATTY	2 cut(s)	1190, 1223
AcuI	CTGAAG	3 cut(s)	771, 1551, 1616
AdeI	CACNNNGTG	1 cut(s)	1082
AfaI	GTAC	4 cut(s)	74, 391, 469, 1601
AfiI	CCNNNNNNNGG	4 cut(s)	692, 818, 1039, 1783
AflII	CTTAAG	1 cut(s)	1559
AflIII	ACRYGT	1 cut(s)	1922
AgsI	TTSAA	9 cut(s)	221, 229, 567, 1151, 1228, 1772, 1781, 1880, 1936
AhlI	ACTAGT	1 cut(s)	1675
AjnI	CCWGG	2 cut(s)	75, 836
AleI	CACNNNNGTG	1 cut(s)	38
AluBI	AGCT	8 cut(s)	26, 333, 502, 1137, 1535, 1698, 1967, 1987
AluI	AGCT	8 cut(s)	26, 333, 502, 1137, 1535, 1698, 1967, 1987
Alw26I	GTCTC	2 cut(s)	182, 194
AlwI	GGATC	1 cut(s)	531
AlwNI	CAGNNNCTG	1 cut(s)	82
AoxI	GGCC	4 cut(s)	183, 762, 1440, 1652
ApeKI	GCWGC	5 cut(s)	82, 302, 671, 1450, 1987
ApoI	RAATTY	2 cut(s)	1190, 1223
AseI	ATTAAT	1 cut(s)	294
Asp700I	GAANNNNTTC	1 cut(s)	2025
Asp718I	GGTACC	1 cut(s)	72
AspA2I	CCTAGG	1 cut(s)	1457
AspLEI	GCGC	2 cut(s)	87, 647
AspS9I	GGNCC	4 cut(s)	369, 763, 1688, 1904
AsuHPI	GGTGA	8 cut(s)	100, 596, 795, 1268, 1414, 1694, 1908, 1997
AsuII	TTCGAA	1 cut(s)	1791
AvaII	GGWCC	3 cut(s)	369, 1688, 1904
AvrII	CCTAGG	1 cut(s)	1457
BanI	GGYRCC	2 cut(s)	72, 575
BanII	GRGCYC	2 cut(s)	54, 813
BauI	CACGAG	1 cut(s)	1138
BbvI	GCAGC	5 cut(s)	69, 289, 683, 1437, 1974
BccI	CCATC	6 cut(s)	713, 982, 1236, 1253, 1427, 1679
BceAI	ACGGC	2 cut(s)	51, 149
BciT130I	CCWGG	2 cut(s)	77, 838
BclI	TGATCA	2 cut(s)	451, 1131
BcoDI	GTCTC	2 cut(s)	182, 194
BcuI	ACTAGT	1 cut(s)	1675
BfaI	CTAG	3 cut(s)	1458, 1650, 1676
BfmI	CTRYAG	3 cut(s)	738, 1065, 1988
BfrI	CTTAAG	1 cut(s)	1559
BglI	GCCNNNNNGGC	1 cut(s)	1061
BglII	AGATCT	1 cut(s)	1704
BisI	GCNGC	5 cut(s)	83, 303, 672, 1451, 1988
BlnI	CCTAGG	1 cut(s)	1457
BlpI	GCTNAGC	2 cut(s)	88, 1014
BlsI	GCNGC	5 cut(s)	84, 304, 673, 1452, 1989
Bme1390I	CCNGG	2 cut(s)	77, 838
Bme18I	GGWCC	3 cut(s)	369, 1688, 1904
BmgT120I	GGNCC	4 cut(s)	369, 763, 1688, 1904
BmiI	GGNNCC	4 cut(s)	51, 74, 577, 936
BmrFI	CCNGG	2 cut(s)	77, 838
BmsI	GCATC	5 cut(s)	271, 555, 1462, 1622, 1922
Bpu10I	CCTNAGC	1 cut(s)	807
Bpu1102I	GCTNAGC	2 cut(s)	88, 1014
Bpu14I	TTCGAA	1 cut(s)	1791
BpuEI	CTTGAG	1 cut(s)	924
BsaJI	CCNNGG	8 cut(s)	54, 76, 325, 435, 505, 836, 1457, 1488
Bsc4I	CCNNNNNNNGG	4 cut(s)	692, 818, 1039, 1783
Bse118I	RCCGGY	1 cut(s)	1442
Bse1I	ACTGG	4 cut(s)	1524, 1633, 1814, 2008
Bse3DI	GCAATG	4 cut(s)	666, 674, 775, 2022
BseBI	CCWGG	2 cut(s)	77, 838
BseDI	CCNNGG	8 cut(s)	54, 76, 325, 435, 505, 836, 1457, 1488
BseLI	CCNNNNNNNGG	4 cut(s)	692, 818, 1039, 1783
BseMI	GCAATG	4 cut(s)	666, 674, 775, 2022
BseMII	CTCAG	6 cut(s)	102, 189, 651, 798, 839, 1005
BseNI	ACTGG	4 cut(s)	1524, 1633, 1814, 2008
BseRI	GAGGAG	2 cut(s)	600, 1320
BseXI	GCAGC	5 cut(s)	69, 289, 683, 1437, 1974
BseYI	CCCAGC	3 cut(s)	43, 1498, 2046
BsgI	GTGCAG	1 cut(s)	80
BshFI	GGCC	4 cut(s)	185, 764, 1442, 1654
BshNI	GGYRCC	2 cut(s)	72, 575
BsiSI	CCGG	2 cut(s)	1214, 1443
BslFI	GGGAC	2 cut(s)	1016, 1401
BslI	CCNNNNNNNGG	4 cut(s)	692, 818, 1039, 1783
BsmAI	GTCTC	2 cut(s)	182, 194
BsmBI	CGTCTC	1 cut(s)	194
BsmFI	GGGAC	2 cut(s)	1016, 1401
BsmI	GAATGC	1 cut(s)	2005
BsnI	GGCC	4 cut(s)	185, 764, 1442, 1654
Bsp119I	TTCGAA	1 cut(s)	1791
Bsp1286I	GDGCHC	2 cut(s)	54, 813
Bsp143I	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
Bsp1720I	GCTNAGC	2 cut(s)	88, 1014
Bsp19I	CCATGG	3 cut(s)	435, 505, 1488
BspACI	CCGC	5 cut(s)	38, 208, 766, 1564, 1858
BspANI	GGCC	4 cut(s)	185, 764, 1442, 1654
BspCNI	CTCAG	6 cut(s)	101, 190, 652, 799, 838, 1006
BspHI	TCATGA	1 cut(s)	1752
BspLI	GGNNCC	4 cut(s)	51, 74, 577, 936
BspMAI	CTGCAG	2 cut(s)	742, 1992
BspPI	GGATC	1 cut(s)	531
BspT104I	TTCGAA	1 cut(s)	1791
BspT107I	GGYRCC	2 cut(s)	72, 575
BspTI	CTTAAG	1 cut(s)	1559
BsrDI	GCAATG	4 cut(s)	666, 674, 775, 2022
BsrFI	RCCGGY	1 cut(s)	1442
BsrI	ACTGG	4 cut(s)	1524, 1633, 1814, 2008
BssAI	RCCGGY	1 cut(s)	1442
BssECI	CCNNGG	8 cut(s)	54, 76, 325, 435, 505, 836, 1457, 1488
BssMI	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
BssNAI	GTATAC	1 cut(s)	1547
BssSI	CACGAG	1 cut(s)	1138
BssT1I	CCWWGG	5 cut(s)	54, 435, 505, 1457, 1488
Bst1107I	GTATAC	1 cut(s)	1547
Bst2BI	CACGAG	1 cut(s)	1138
Bst2UI	CCWGG	2 cut(s)	77, 838
Bst4CI	ACNGT	4 cut(s)	535, 690, 862, 1429
Bst6I	CTCTTC	5 cut(s)	139, 154, 261, 477, 1090
BstAFI	CTTAAG	1 cut(s)	1559
BstBI	TTCGAA	1 cut(s)	1791
BstC8I	GCNNGC	3 cut(s)	766, 1320, 1495
BstDEI	CTNAG	6 cut(s)	88, 198, 660, 807, 825, 1014
BstDSI	CCRYGG	4 cut(s)	325, 435, 505, 1488
BstENI	CCTNNNNNAGG	1 cut(s)	1781
BstHHI	GCGC	2 cut(s)	87, 647
BstKTI	GATC	7 cut(s)	445, 454, 526, 589, 1105, 1134, 1707
BstMAI	GTCTC	2 cut(s)	182, 194
BstMBI	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
BstMWI	GCNNNNNNNGC	6 cut(s)	49, 58, 191, 614, 1061, 1499
BstNI	CCWGG	2 cut(s)	77, 838
BstNSI	RCATGY	1 cut(s)	1497
BstSCI	CCNGG	2 cut(s)	75, 836
BstSFI	CTRYAG	3 cut(s)	738, 1065, 1988
BstV1I	GCAGC	5 cut(s)	69, 289, 683, 1437, 1974
BstX2I	RGATCY	2 cut(s)	523, 1704
BstXI	CCANNNNNNTGG	1 cut(s)	2054
BstYI	RGATCY	2 cut(s)	523, 1704
BstZ17I	GTATAC	1 cut(s)	1547
BsuRI	GGCC	4 cut(s)	185, 764, 1442, 1654
BtgI	CCRYGG	4 cut(s)	325, 435, 505, 1488
BtsI	GCAGTG	1 cut(s)	1408
BtsIMutI	CAGTG	3 cut(s)	867, 1408, 1517
Cac8I	GCNNGC	3 cut(s)	766, 1320, 1495
CaiI	CAGNNNCTG	1 cut(s)	82
CciI	TCATGA	1 cut(s)	1752
CfoI	GCGC	2 cut(s)	87, 647
Cfr10I	RCCGGY	1 cut(s)	1442
Cfr13I	GGNCC	4 cut(s)	369, 763, 1688, 1904
CseI	GACGC	1 cut(s)	1403
Csp6I	GTAC	4 cut(s)	73, 390, 468, 1600
CspCI	CAANNNNNGTGG	2 cut(s)	1414, 1449
CviQI	GTAC	4 cut(s)	73, 390, 468, 1600
DdeI	CTNAG	6 cut(s)	88, 198, 660, 807, 825, 1014
DpnI	GATC	7 cut(s)	444, 453, 525, 588, 1104, 1133, 1706
DpnII	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
DraI	TTTAAA	1 cut(s)	847
DraIII	CACNNNGTG	1 cut(s)	1082
DrdI	GACNNNNNNGTC	1 cut(s)	688
DseDI	GACNNNNNNGTC	1 cut(s)	688
Eam1104I	CTCTTC	5 cut(s)	139, 154, 261, 477, 1090
EarI	CTCTTC	5 cut(s)	139, 154, 261, 477, 1090
Eco130I	CCWWGG	5 cut(s)	54, 435, 505, 1457, 1488
Eco24I	GRGCYC	2 cut(s)	54, 813
Eco32I	GATATC	1 cut(s)	1045
Eco47I	GGWCC	3 cut(s)	369, 1688, 1904
Eco57I	CTGAAG	3 cut(s)	771, 1551, 1616
EcoNI	CCTNNNNNAGG	1 cut(s)	1781
EcoO109I	RGGNCCY	1 cut(s)	369
EcoRI	GAATTC	1 cut(s)	1223
EcoRII	CCWGG	2 cut(s)	75, 836
EcoRV	GATATC	1 cut(s)	1045
EcoT14I	CCWWGG	5 cut(s)	54, 435, 505, 1457, 1488
EcoT38I	GRGCYC	2 cut(s)	54, 813
ErhI	CCWWGG	5 cut(s)	54, 435, 505, 1457, 1488
Esp3I	CGTCTC	1 cut(s)	194
FaqI	GGGAC	2 cut(s)	1016, 1401
FauI	CCCGC	2 cut(s)	773, 1557
FbaI	TGATCA	2 cut(s)	451, 1131
FblI	GTMKAC	2 cut(s)	1467, 1546
Fnu4HI	GCNGC	5 cut(s)	83, 303, 672, 1451, 1988
FriOI	GRGCYC	2 cut(s)	54, 813
Fsp4HI	GCNGC	5 cut(s)	83, 303, 672, 1451, 1988
FspBI	CTAG	3 cut(s)	1458, 1650, 1676
FspI	TGCGCA	1 cut(s)	646
GlaI	GCGC	2 cut(s)	86, 646
GluI	GCNGC	5 cut(s)	83, 303, 672, 1451, 1988
GsaI	CCCAGC	3 cut(s)	47, 1502, 2050
HaeIII	GGCC	4 cut(s)	185, 764, 1442, 1654
HapII	CCGG	2 cut(s)	1214, 1443
HgaI	GACGC	1 cut(s)	1403
HhaI	GCGC	2 cut(s)	87, 647
Hin6I	GCGC	2 cut(s)	85, 645
HinP1I	GCGC	2 cut(s)	85, 645
HincII	GTYRAC	1 cut(s)	1891
HindII	GTYRAC	1 cut(s)	1891
HinfI	GANTC	6 cut(s)	682, 743, 800, 992, 1607, 1835
HpaII	CCGG	2 cut(s)	1214, 1443
HphI	GGTGA	8 cut(s)	100, 596, 795, 1268, 1414, 1694, 1908, 1997
Hpy166II	GTNNAC	4 cut(s)	1468, 1547, 1820, 1891
Hpy188I	TCNGA	8 cut(s)	23, 251, 516, 922, 1160, 1299, 1596, 1704
Hpy188III	TCNNGA	7 cut(s)	693, 733, 749, 1753, 1772, 1880, 1907
Hpy8I	GTNNAC	4 cut(s)	1468, 1547, 1820, 1891
Hpy99I	CGWCG	5 cut(s)	10, 13, 16, 19, 170
HpyAV	CCTTC	7 cut(s)	360, 388, 969, 1513, 1579, 1759, 1895
HpyCH4III	ACNGT	4 cut(s)	535, 690, 862, 1429
HpyCH4IV	ACGT	8 cut(s)	17, 358, 494, 600, 1081, 1816, 1924, 1958
HpyF10VI	GCNNNNNNNGC	6 cut(s)	49, 58, 191, 614, 1061, 1499
HpyF3I	CTNAG	6 cut(s)	88, 198, 660, 807, 825, 1014
HpySE526I	ACGT	8 cut(s)	17, 358, 494, 600, 1081, 1816, 1924, 1958
HspAI	GCGC	2 cut(s)	85, 645
KpnI	GGTACC	1 cut(s)	76
Ksp22I	TGATCA	2 cut(s)	451, 1131
Kzo9I	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
LmnI	GCTCC	7 cut(s)	23, 49, 330, 507, 613, 622, 934
Lsp1109I	GCAGC	5 cut(s)	69, 289, 683, 1437, 1974
LweI	GCATC	5 cut(s)	271, 555, 1462, 1622, 1922
MaeI	CTAG	3 cut(s)	1458, 1650, 1676
MaeII	ACGT	8 cut(s)	17, 358, 494, 600, 1081, 1816, 1924, 1958
MaeIII	GTNAC	4 cut(s)	754, 856, 1072, 1925
MalI	GATC	7 cut(s)	444, 453, 525, 588, 1104, 1133, 1706
MboI	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
MboII	GAAGA	6 cut(s)	126, 135, 141, 278, 464, 1107
MflI	RGATCY	2 cut(s)	523, 1704
MhlI	GDGCHC	2 cut(s)	54, 813
MluCI	AATT	7 cut(s)	422, 462, 775, 1190, 1223, 1348, 2032
MlyI	GAGTC	2 cut(s)	676, 1844
MmeI	TCCRAC	1 cut(s)	1277
MroXI	GAANNNNTTC	1 cut(s)	2025
MseI	TTAA	9 cut(s)	261, 294, 318, 591, 846, 948, 1059, 1325, 1560
MslI	CAYNNNNRTG	1 cut(s)	38
MspCI	CTTAAG	1 cut(s)	1559
MspI	CCGG	2 cut(s)	1214, 1443
MspR9I	CCNGG	2 cut(s)	77, 838
Mva1269I	GAATGC	1 cut(s)	2005
MvaI	CCWGG	2 cut(s)	77, 838
MwoI	GCNNNNNNNGC	6 cut(s)	49, 58, 191, 614, 1061, 1499
NcoI	CCATGG	3 cut(s)	435, 505, 1488
NdeII	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
NlaIV	GGNNCC	4 cut(s)	51, 74, 577, 936
NmeAIII	GCCGAG	1 cut(s)	211
NsbI	TGCGCA	1 cut(s)	646
NspI	RCATGY	1 cut(s)	1497
NspV	TTCGAA	1 cut(s)	1791
OliI	CACNNNNGTG	1 cut(s)	38
PaeI	GCATGC	1 cut(s)	1497
PagI	TCATGA	1 cut(s)	1752
PcsI	WCGNNNNNNNCGW	3 cut(s)	11, 14, 284
PctI	GAATGC	1 cut(s)	2005
PdmI	GAANNNNTTC	1 cut(s)	2025
PfeI	GAWTC	4 cut(s)	743, 800, 992, 1607
PflMI	CCANNNNNTGG	1 cut(s)	692
PkrI	GCNGC	5 cut(s)	84, 304, 673, 1452, 1989
PleI	GAGTC	2 cut(s)	676, 1843
PpsI	GAGTC	2 cut(s)	676, 1843
PpuMI	RGGWCCY	1 cut(s)	369
PshBI	ATTAAT	1 cut(s)	294
PsiI	TTATAA	1 cut(s)	1619
Psp1406I	AACGTT	1 cut(s)	600
Psp5II	RGGWCCY	1 cut(s)	369
Psp6I	CCWGG	2 cut(s)	75, 836
PspFI	CCCAGC	3 cut(s)	43, 1498, 2046
PspGI	CCWGG	2 cut(s)	75, 836
PspN4I	GGNNCC	4 cut(s)	51, 74, 577, 936
PspPI	GGNCC	4 cut(s)	369, 763, 1688, 1904
PspPPI	RGGWCCY	1 cut(s)	369
PsrI	GAACNNNNNNTAC	2 cut(s)	1530, 1562
PstI	CTGCAG	2 cut(s)	742, 1992
PstNI	CAGNNNCTG	1 cut(s)	82
PsuI	RGATCY	2 cut(s)	523, 1704
RsaI	GTAC	4 cut(s)	74, 391, 469, 1601
RsaNI	GTAC	4 cut(s)	73, 390, 468, 1600
RseI	CAYNNNNRTG	1 cut(s)	38
SaqAI	TTAA	9 cut(s)	261, 294, 318, 591, 846, 948, 1059, 1325, 1560
SatI	GCNGC	5 cut(s)	83, 303, 672, 1451, 1988
Sau3AI	GATC	7 cut(s)	442, 451, 523, 586, 1102, 1131, 1704
Sau96I	GGNCC	4 cut(s)	369, 763, 1688, 1904
SchI	GAGTC	2 cut(s)	676, 1844
ScrFI	CCNGG	2 cut(s)	77, 838
SduI	GDGCHC	2 cut(s)	54, 813
SfaNI	GCATC	5 cut(s)	271, 555, 1462, 1622, 1922
SfcI	CTRYAG	3 cut(s)	738, 1065, 1988
SfuI	TTCGAA	1 cut(s)	1791
SinI	GGWCC	3 cut(s)	369, 1688, 1904
SmiMI	CAYNNNNRTG	1 cut(s)	38
SmlI	CTYRAG	2 cut(s)	939, 1559
SmoI	CTYRAG	2 cut(s)	939, 1559
SpeI	ACTAGT	1 cut(s)	1675
SphI	GCATGC	1 cut(s)	1497
Sse9I	AATT	7 cut(s)	422, 462, 775, 1190, 1223, 1348, 2032
SsiI	CCGC	5 cut(s)	38, 208, 766, 1564, 1858
SspI	AATATT	2 cut(s)	225, 1127
SspMI	CTAG	3 cut(s)	1458, 1650, 1676
StyD4I	CCNGG	2 cut(s)	75, 836
StyI	CCWWGG	5 cut(s)	54, 435, 505, 1457, 1488
TaaI	ACNGT	4 cut(s)	535, 690, 862, 1429
TaiI	ACGT	8 cut(s)	20, 361, 497, 603, 1084, 1819, 1927, 1961
TaqI	TCGA	3 cut(s)	160, 1570, 1791
TasI	AATT	7 cut(s)	422, 462, 775, 1190, 1223, 1348, 2032
TatI	WGTACW	1 cut(s)	1599
TfiI	GAWTC	4 cut(s)	743, 800, 992, 1607
Tru1I	TTAA	9 cut(s)	261, 294, 318, 591, 846, 948, 1059, 1325, 1560
Tru9I	TTAA	9 cut(s)	261, 294, 318, 591, 846, 948, 1059, 1325, 1560
TscAI	CASTG	3 cut(s)	867, 1408, 1524
TseI	GCWGC	5 cut(s)	82, 302, 671, 1450, 1987
TspGWI	ACGGA	4 cut(s)	127, 293, 1193, 1197
TspRI	CASTG	3 cut(s)	867, 1408, 1524
Van91I	CCANNNNNTGG	1 cut(s)	692
Vha464I	CTTAAG	1 cut(s)	1559
VpaK11BI	GGWCC	3 cut(s)	369, 1688, 1904
VspI	ATTAAT	1 cut(s)	294
XagI	CCTNNNNNAGG	1 cut(s)	1781
XapI	RAATTY	2 cut(s)	1190, 1223
XceI	RCATGY	1 cut(s)	1497
XmaJI	CCTAGG	1 cut(s)	1457
XmiI	GTMKAC	2 cut(s)	1467, 1546
XmnI	GAANNNNTTC	1 cut(s)	2025
XspI	CTAG	3 cut(s)	1458, 1650, 1676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.