Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

28817256 .. 28823148

Gene Structure 5893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1199600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2874 bp

ATGGATAATCTGAAGGAGCCTCTGTTGAAGCCTTTGGATATTGATAACAAAGATAAAAGAATTAGAACAGTGAAGTTTAAAATAGGGGATATCGAGTGTGCATCTTGTGCAACTACCATTGAATCTGTGTTGGGAAAGCTTGATGGGATCAAGAGTGCAACAGTGTCGCCCATTGAAGGCCAAGCTGCAGTCAATTATATACCAGTGCTCATTAATGCAAGAAAAATTAAGGAAGCTGTAGAAGACGCTGGTTTCCCTGTTTCTGAATTTCCAGAACAAGATGTAGCAGTATGCCGACTGAGGATAAAAGGAATGGCATGCACCAGCTGCTCCGAGTCTGTTGAGTGCGCTCTTCGAATGGTCGATGGAGTGAAAAATGCAGTTGTTGGTCTAGCCCTTGAAGAAGCAAAGGTTCACTTTGATCCCAACCTCACTGATACTAGTTGCATTATTCAAGCAATTGAAGATGCTGGCTTTGGGGCTGAACTGATTAGTTCTGGGAATGATGTCAGCAAACTTCATCTAAAACTTGAAGGAATTGATTCTCCAGAGGATATGACTATTGTCCAATCCTCCGTTGAATCAGTTGGAGGAGTAAGTAATGTTGAAGTCAACTTGGCAGAGAAGAAGGTGACTATTACCTATGATTCAGACCTCACAGGTCCAAGATCTCTTATACGTTGTATTGAAGAAGCTGGCCGTGAATCCAAGGTGTACCAGGCAAGCTTGTATGTTCCTCCAAGACCAAGAGAAGCTGAGCGGAAACATGAGATTCAGATGTACAGGAACCAGTTTTTCCTTAGCTGCCTATTTTCGGTTCCTATATTTTTATTCTCAATGGTGCTTCCGATGCTTCCTCCTTATGGAAACTGGTTAGACTACAAGGTTCACAATATGCTCACAGTAGGGATGCTTTTAAGATGGATTCTTTGCACACCAGTGCAGTTCATTGTTGGCCGGAGGTTCTATGTTGGATCATATCATGCATTGAGACGTAGATCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTTTACTCTGTCTATATTGCAATAAAAGCATTTGCTTTGGATAAATTTGAAGGGCAAGATTTCTTTGAAACTAGTTCTATGTTGATATCCTTTATACTCTTGGGAAAGTACTTAGAAGTTCTGGCTAAAGGGAAGACATCTGATGCTTTGGCGAAGCTGACAGACCTTGCTCCTGATACGGCTTTTCTGTTGTCATTAGATGACGATGACAATGTTATCTCAGAGATCGAAATCAGCACTCAACTTATTCAAAAAAATGATATACTTAAGGTTGCTCCGGGGGCAAAAGTTCCTGTTGATGGGATTGTAGTTCGTGGTCACAGCTATGTGAATGAGAGTATGATCACAGGAGAGGCAACACCCATTTCTAAAAAACTTGGTGACAAGGTAATTGGTGGCACTATGAATGAGAATGGATGCTTACTGGTGAAGGCAACTCATGTTGGTTCGGAGACTGCACTTTCCCAAATAGTTCAACTTGTTGAAGCTGCACAGCTTGCCAGAGCACCTGTTCAGAAACTAGCTGACCAGATTTCAAAGTTTTTTGTTCCAACAGTTGTTATTGTAGCATTTTTGACATGGCTTGGATGGTTCATCCCTGGAGAATTTGGTCTTTTCCCGGAAAACTGGATACCAAAAGGCATGGATAAATTTGAGCTGGCATTACAATTCGGTATCTCAGTATTAGTAGTTGCTTGCCCTTGTGCCTTGGGACTAGCAACCCCTACAGCAGTAATGGTTGCAACAGGGAAGGGTGCTTCGCAAGGTGTTCTTATCAAGGGTGGAAATGCACTCGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAAACTGGGACTCTGACAGTTGGGAAACCCGTGGTAGTCAATGCAGTGCTCTTCTCCAGTTACTCAATGGAGGAGTTCTGTGCTGTCTCTATTGCCACAGAGGCAAATAGTGAACACCCAATAGCGAAATCGGTGGTGGAGCATGCTAAGAGGCTGCTAAAGACGTTTGGATCAACTGAACATGTAATGGAGGCTAAGGATTTTGAGGTGCACACAGGAGCTGGCGTTAGTGGAAGAGTTGGTGAAAAAATGGTTTTGGTTGGGAACAAGAGACTCATGCGGCATAATAATGTCCAGGTTGGTCCTGAGGTTGAGAAATATGTTTCGGAACATGAGAATCTGGCTCGAACATGTGTGTTGGTTGCCATTGATGGAAAGATTGCGGGGTCTTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCGCGGGTCATCTCTTATCTTCACTCGATGAACATTACCAGCATCATGGTTACTGGTGATAACTGGGCTACAGCAGCAGCCATCGCAAAGGAGGTTGGCATTAATAAGGTGTATGCTGAGACGGATCCACTGGGAAAAGCTGATAGAATCAAAGAATTGCAGATGAAAGGAATGGCGGTGGCAATGGTGGGAGATGGAATCAATGACTCTCCAGCTTTGGTTGCAGCAGATGTTGGCATGGCAATTGGTGCTGGCACCGACGTAGCTATAGAAGCCGCTGATATAGTTCTGATGAAGAGCAACTTGGAAGATGTAGTTACAGCCATAGATCTCTCCAGAAAAACTATGTCACGAATTCGATTGAATTATGTCTGGGCCCTGGGCTACAATATCCTCGGCATGCCAGTCGCAGCTGGAGTCTTGTTCCCTTTCACTGGAATTCGACTACCACCATGGCTTGCCGGTGCATGTATGGCTGCTTCATCTCTCAGCGTGGTATGTTCTTCTCTCTTGTTGCAGTCATATAAGAAGCCCTTGCATATTCAGAGCGTCTCGAACGGCAAGTTTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

958

Amino Acids

103.37

Weight (kDa)

5.99

Isoelectric Point (pI)

36.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	26 - 85	1e-12	Heavy-metal-associated domain
HMA	PF00403	100 - 159	3.7e-09	Heavy-metal-associated domain
HMA	PF00403	189 - 235	1.3e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	424 - 511	8.2e-25	P-type ATPase actuator domain
Hydrolase	PF00702	620 - 844	5.7e-34	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1031, 2555
AccBSI	CCGCTC	1 cut(s)	760
AccII	CGCG	1 cut(s)	2305
AciI	CCGC	6 cut(s)	760, 2158, 2261, 2305, 2477, 2577
AclWI	GGATC	7 cut(s)	155, 416, 982, 2056, 2276, 2420, 2433
AcoI	YGGCCR	2 cut(s)	697, 955
AcsI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2655, 2739
AcuI	CTGAAG	1 cut(s)	32
AfaI	GTAC	3 cut(s)	716, 782, 1157
AfiI	CCNNNNNNNGG	5 cut(s)	176, 814, 863, 1346, 2735
AflII	CTTAAG	1 cut(s)	1313
AflIII	ACRYGT	2 cut(s)	2059, 2228
AhlI	ACTAGT	2 cut(s)	440, 1118
AjnI	CCWGG	4 cut(s)	717, 1645, 2172, 2679
AjuI	GAANNNNNNNTTGG	2 cut(s)	2588, 2620
AleI	CACNNNNGTG	1 cut(s)	938
Alw21I	GWGCWC	4 cut(s)	210, 1555, 1929, 2091
Alw26I	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2857
Alw44I	GTGCAC	1 cut(s)	2087
AlwI	GGATC	7 cut(s)	155, 416, 982, 2056, 2276, 2420, 2433
AlwNI	CAGNNNCTG	2 cut(s)	2099, 2300
AoxI	GGCC	4 cut(s)	178, 697, 955, 2676
ApaI	GGGCCC	1 cut(s)	2680
ApaLI	GTGCAC	1 cut(s)	2087
ApoI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2655, 2739
AseI	ATTAAT	2 cut(s)	213, 2403
Asp700I	GAANNNNTTC	1 cut(s)	541
AspLEI	GCGC	2 cut(s)	350, 2305
AspS9I	GGNCC	4 cut(s)	662, 2180, 2676, 2677
AsuC2I	CCSGG	2 cut(s)	1326, 1667
AsuHPI	GGTGA	6 cut(s)	643, 1439, 1486, 1870, 2132, 2369
AsuII	TTCGAA	1 cut(s)	355
AvaII	GGWCC	2 cut(s)	662, 2180
AxyI	CCTNAGG	1 cut(s)	2184
BaeGI	GKGCMC	3 cut(s)	1034, 2091, 2680
BamHI	GGATCC	1 cut(s)	2425
BanI	GGYRCC	2 cut(s)	1031, 2555
BanII	GRGCYC	1 cut(s)	2680
BarI	GAAGNNNNNNTAC	2 cut(s)	2764, 2796
BbsI	GAAGAC	2 cut(s)	249, 1187
Bbv12I	GWGCWC	4 cut(s)	210, 1555, 1929, 2091
BccI	CCATC	8 cut(s)	137, 359, 915, 1340, 1629, 2243, 2390, 2489
BceAI	ACGGC	2 cut(s)	684, 1242
BcgI	CGANNNNNNTGC	4 cut(s)	147, 181, 2689, 2723
BciT130I	CCWGG	4 cut(s)	719, 1647, 2174, 2681
BciVI	GTATCC	1 cut(s)	1671
BclI	TGATCA	1 cut(s)	1389
BcnI	CCSGG	2 cut(s)	1326, 1667
BcoDI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2857
BcuI	ACTAGT	2 cut(s)	440, 1118
BfaI	CTAG	5 cut(s)	392, 441, 1119, 1568, 1763
BfmI	CTRYAG	5 cut(s)	186, 237, 1773, 2370, 2568
BfrI	CTTAAG	1 cut(s)	1313
BfuI	GTATCC	1 cut(s)	1671
BglI	GCCNNNNNGGC	1 cut(s)	1979
BglII	AGATCT	2 cut(s)	668, 2629
BlpI	GCTNAGC	1 cut(s)	756
BmcAI	AGTACT	1 cut(s)	1157
Bme1390I	CCNGG	6 cut(s)	719, 1326, 1647, 1667, 2174, 2681
Bme18I	GGWCC	2 cut(s)	662, 2180
BmgT120I	GGNCC	4 cut(s)	662, 2180, 2676, 2677
BmiI	GGNNCC	7 cut(s)	18, 788, 819, 1033, 2427, 2557, 2678
BmrFI	CCNGG	6 cut(s)	719, 1326, 1647, 1667, 2174, 2681
BmrI	ACTGGG	4 cut(s)	1037, 1893, 2374, 2441
BmsI	GCATC	7 cut(s)	110, 457, 840, 900, 1180, 1454, 2352
BmuI	ACTGGG	4 cut(s)	1037, 1893, 2374, 2441
BoxI	GACNNNNGTC	1 cut(s)	563
BpiI	GAAGAC	2 cut(s)	249, 1187
BpmI	CTGGAG	6 cut(s)	531, 1668, 1918, 2496, 2620, 2736
Bpu10I	CCTNAGC	2 cut(s)	800, 2073
Bpu1102I	GCTNAGC	1 cut(s)	756
Bpu14I	TTCGAA	1 cut(s)	355
BpuMI	CCSGG	2 cut(s)	1326, 1667
BsaBI	GATNNNNATC	1 cut(s)	1277
BsaJI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2679, 2680, 2695, 2753
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 2088, 2118
Bsc4I	CCNNNNNNNGG	5 cut(s)	176, 814, 863, 1346, 2735
Bse118I	RCCGGY	1 cut(s)	2762
Bse21I	CCTNAGG	1 cut(s)	2184
Bse3DI	GCAATG	1 cut(s)	2490
Bse8I	GATNNNNATC	1 cut(s)	1277
BseBI	CCWGG	4 cut(s)	719, 1647, 2174, 2681
BseDI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2679, 2680, 2695, 2753
BseGI	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BseJI	GATNNNNATC	1 cut(s)	1277
BseLI	CCNNNNNNNGG	5 cut(s)	176, 814, 863, 1346, 2735
BseMI	GCAATG	1 cut(s)	2490
BseMII	CTCAG	7 cut(s)	290, 747, 1281, 1740, 2175, 2409, 2803
BseRI	GAGGAG	2 cut(s)	606, 1964
BseSI	GKGCMC	3 cut(s)	1034, 2091, 2680
BsgI	GTGCAG	3 cut(s)	962, 1488, 1521
Bsh1236I	CGCG	1 cut(s)	2305
BshFI	GGCC	4 cut(s)	180, 699, 957, 2678
BshNI	GGYRCC	2 cut(s)	1031, 2555
BsiHKAI	GWGCWC	4 cut(s)	210, 1555, 1929, 2091
BsiSI	CCGG	4 cut(s)	958, 1325, 1667, 2763
BslFI	GGGAC	2 cut(s)	1773, 1900
BslI	CCNNNNNNNGG	5 cut(s)	176, 814, 863, 1346, 2735
BsmAI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2857
BsmBI	CGTCTC	3 cut(s)	985, 2414, 2857
BsmFI	GGGAC	2 cut(s)	1773, 1900
BsnI	GGCC	4 cut(s)	180, 699, 957, 2678
Bsp119I	TTCGAA	1 cut(s)	355
Bsp120I	GGGCCC	1 cut(s)	2676
Bsp1286I	GDGCHC	6 cut(s)	210, 1034, 1555, 1929, 2091, 2680
Bsp1407I	TGTACA	1 cut(s)	780
Bsp1720I	GCTNAGC	1 cut(s)	756
Bsp19I	CCATGG	1 cut(s)	2753
BspACI	CCGC	6 cut(s)	760, 2158, 2261, 2305, 2477, 2577
BspANI	GGCC	4 cut(s)	180, 699, 957, 2678
BspCNI	CTCAG	7 cut(s)	291, 748, 1280, 1739, 2176, 2410, 2802
BspFNI	CGCG	1 cut(s)	2305
BspLI	GGNNCC	7 cut(s)	18, 788, 819, 1033, 2427, 2557, 2678
BspMAI	CTGCAG	1 cut(s)	190
BspPI	GGATC	7 cut(s)	155, 416, 982, 2056, 2276, 2420, 2433
BspQI	GCTCTTC	3 cut(s)	357, 1934, 2591
BspT104I	TTCGAA	1 cut(s)	355
BspT107I	GGYRCC	2 cut(s)	1031, 2555
BspTI	CTTAAG	1 cut(s)	1313
BsrBI	CCGCTC	1 cut(s)	760
BsrDI	GCAATG	1 cut(s)	2490
BsrFI	RCCGGY	1 cut(s)	2762
BsrGI	TGTACA	1 cut(s)	780
BssAI	RCCGGY	1 cut(s)	2762
BssECI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2679, 2680, 2695, 2753
BssT1I	CCWWGG	3 cut(s)	708, 1755, 2753
Bst2UI	CCWGG	4 cut(s)	719, 1647, 2174, 2681
Bst4CI	ACNGT	6 cut(s)	70, 163, 904, 1603, 1867, 1897
Bst6I	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
BstAFI	CTTAAG	1 cut(s)	1313
BstAPI	GCANNNNNTGC	4 cut(s)	107, 327, 1544, 2549
BstAUI	TGTACA	1 cut(s)	780
BstBI	TTCGAA	1 cut(s)	355
BstDSI	CCRYGG	2 cut(s)	1908, 2753
BstF5I	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BstFNI	CGCG	1 cut(s)	2305
BstHHI	GCGC	2 cut(s)	350, 2305
BstMAI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2857
BstNI	CCWGG	4 cut(s)	719, 1647, 2174, 2681
BstNSI	RCATGY	6 cut(s)	321, 2024, 2063, 2232, 2704, 2772
BstPAI	GACNNNNGTC	1 cut(s)	563
BstSCI	CCNGG	6 cut(s)	717, 1324, 1645, 1665, 2172, 2679
BstSFI	CTRYAG	5 cut(s)	186, 237, 1773, 2370, 2568
BstSLI	GKGCMC	3 cut(s)	1034, 2091, 2680
BstUI	CGCG	1 cut(s)	2305
BstV2I	GAAGAC	2 cut(s)	249, 1187
BstX2I	RGATCY	3 cut(s)	668, 2425, 2629
BstXI	CCANNNNNNTGG	1 cut(s)	2347
BstYI	RGATCY	3 cut(s)	668, 2425, 2629
Bsu36I	CCTNAGG	1 cut(s)	2184
BsuI	GTATCC	1 cut(s)	1671
BsuRI	GGCC	4 cut(s)	180, 699, 957, 2678
BtgI	CCRYGG	2 cut(s)	1908, 2753
BtgZI	GCGATG	1 cut(s)	2368
BtsCI	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BtsI	GCAGTG	1 cut(s)	1929
CaiI	CAGNNNCTG	2 cut(s)	2099, 2300
CfoI	GCGC	2 cut(s)	350, 2305
Cfr10I	RCCGGY	1 cut(s)	2762
Cfr13I	GGNCC	4 cut(s)	662, 2180, 2676, 2677
CseI	GACGC	2 cut(s)	254, 2839
Csp6I	GTAC	3 cut(s)	715, 781, 1156
CviQI	GTAC	3 cut(s)	715, 781, 1156
DraI	TTTAAA	1 cut(s)	79
EaeI	YGGCCR	2 cut(s)	697, 955
Eam1104I	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
EarI	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
Eco130I	CCWWGG	3 cut(s)	708, 1755, 2753
Eco24I	GRGCYC	1 cut(s)	2680
Eco32I	GATATC	2 cut(s)	91, 1134
Eco47I	GGWCC	2 cut(s)	662, 2180
Eco57I	CTGAAG	1 cut(s)	32
Eco81I	CCTNAGG	1 cut(s)	2184
EcoO109I	RGGNCCY	1 cut(s)	2677
EcoRI	GAATTC	2 cut(s)	2655, 2739
EcoRII	CCWGG	4 cut(s)	717, 1645, 2172, 2679
EcoRV	GATATC	2 cut(s)	91, 1134
EcoT14I	CCWWGG	3 cut(s)	708, 1755, 2753
EcoT22I	ATGCAT	1 cut(s)	988
EcoT38I	GRGCYC	1 cut(s)	2680
ErhI	CCWWGG	3 cut(s)	708, 1755, 2753
Esp3I	CGTCTC	3 cut(s)	985, 2414, 2857
FalI	AAGNNNNNCTT	8 cut(s)	401, 433, 1803, 1835, 2588, 2620, 2819, 2851
FaqI	GGGAC	2 cut(s)	1773, 1900
FauI	CCCGC	2 cut(s)	2254, 2298
FbaI	TGATCA	1 cut(s)	1389
FokI	GGATG	5 cut(s)	922, 1025, 1476, 1628, 1647
FriOI	GRGCYC	1 cut(s)	2680
FspBI	CTAG	5 cut(s)	392, 441, 1119, 1568, 1763
GlaI	GCGC	2 cut(s)	349, 2304
GsuI	CTGGAG	6 cut(s)	531, 1668, 1918, 2496, 2620, 2736
HaeIII	GGCC	4 cut(s)	180, 699, 957, 2678
HapII	CCGG	4 cut(s)	958, 1325, 1667, 2763
HgaI	GACGC	2 cut(s)	254, 2839
HhaI	GCGC	2 cut(s)	350, 2305
Hin6I	GCGC	2 cut(s)	348, 2303
HinP1I	GCGC	2 cut(s)	348, 2303
HincII	GTYRAC	1 cut(s)	613
HindII	GTYRAC	1 cut(s)	613
HindIII	AAGCTT	2 cut(s)	137, 724
HpaII	CCGG	4 cut(s)	958, 1325, 1667, 2763
HphI	GGTGA	6 cut(s)	643, 1439, 1486, 1870, 2132, 2369
Hpy166II	GTNNAC	6 cut(s)	415, 613, 715, 889, 1991, 2089
Hpy188III	TCNNGA	8 cut(s)	151, 272, 548, 1220, 2183, 2637, 2652, 2854
Hpy8I	GTNNAC	6 cut(s)	415, 613, 715, 889, 1991, 2089
Hpy99I	CGWCG	1 cut(s)	2564
HpyAV	CCTTC	7 cut(s)	7, 170, 527, 622, 1091, 1471, 1792
HpyCH4III	ACNGT	6 cut(s)	70, 163, 904, 1603, 1867, 1897
HpyCH4IV	ACGT	4 cut(s)	679, 994, 2042, 2562
HpySE526I	ACGT	4 cut(s)	679, 994, 2042, 2562
HspAI	GCGC	2 cut(s)	348, 2303
Ksp22I	TGATCA	1 cut(s)	1389
LguI	GCTCTTC	3 cut(s)	357, 1934, 2591
LmnI	GCTCC	6 cut(s)	16, 335, 1222, 1327, 2017, 2096
LweI	GCATC	7 cut(s)	110, 457, 840, 900, 1180, 1454, 2352
MaeI	CTAG	5 cut(s)	392, 441, 1119, 1568, 1763
MaeII	ACGT	4 cut(s)	679, 994, 2042, 2562
MaeIII	GTNAC	8 cut(s)	631, 1364, 1427, 1937, 2275, 2350, 2617, 2649
MbiI	CCGCTC	1 cut(s)	760
MfeI	CAATTG	2 cut(s)	459, 2544
MflI	RGATCY	3 cut(s)	668, 2425, 2629
MhlI	GDGCHC	6 cut(s)	210, 1034, 1555, 1929, 2091, 2680
MlyI	GAGTC	5 cut(s)	344, 1882, 2145, 2501, 2727
MmeI	TCCRAC	3 cut(s)	568, 952, 1622
Mph1103I	ATGCAT	1 cut(s)	988
MroXI	GAANNNNTTC	1 cut(s)	541
MseI	TTAA	6 cut(s)	78, 213, 228, 917, 1314, 2403
MslI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
MspA1I	CMGCKG	3 cut(s)	327, 2579, 2714
MspCI	CTTAAG	1 cut(s)	1313
MspI	CCGG	4 cut(s)	958, 1325, 1667, 2763
MspR9I	CCNGG	6 cut(s)	719, 1326, 1647, 1667, 2174, 2681
MunI	CAATTG	2 cut(s)	459, 2544
MvaI	CCWGG	4 cut(s)	719, 1647, 2174, 2681
MvnI	CGCG	1 cut(s)	2305
NciI	CCSGG	2 cut(s)	1326, 1667
NcoI	CCATGG	1 cut(s)	2753
NlaIV	GGNNCC	7 cut(s)	18, 788, 819, 1033, 2427, 2557, 2678
NmeAIII	GCCGAG	1 cut(s)	2676
NmuCI	GTSAC	4 cut(s)	631, 1364, 1427, 2649
NsiI	ATGCAT	1 cut(s)	988
NspI	RCATGY	6 cut(s)	321, 2024, 2063, 2232, 2704, 2772
NspV	TTCGAA	1 cut(s)	355
OliI	CACNNNNGTG	1 cut(s)	938
PaeI	GCATGC	3 cut(s)	321, 2024, 2704
PasI	CCCWGGG	1 cut(s)	2680
PciI	ACATGT	2 cut(s)	2059, 2228
PciSI	GCTCTTC	3 cut(s)	357, 1934, 2591
PdmI	GAANNNNTTC	1 cut(s)	541
PfoI	TCCNGGA	1 cut(s)	1665
PleI	GAGTC	5 cut(s)	343, 1882, 2145, 2501, 2726
PpsI	GAGTC	5 cut(s)	343, 1882, 2145, 2501, 2726
PscI	ACATGT	2 cut(s)	2059, 2228
PshAI	GACNNNNGTC	1 cut(s)	563
PshBI	ATTAAT	2 cut(s)	213, 2403
Psp6I	CCWGG	4 cut(s)	717, 1645, 2172, 2679
PspGI	CCWGG	4 cut(s)	717, 1645, 2172, 2679
PspN4I	GGNNCC	7 cut(s)	18, 788, 819, 1033, 2427, 2557, 2678
PspOMI	GGGCCC	1 cut(s)	2676
PspPI	GGNCC	4 cut(s)	662, 2180, 2676, 2677
PstI	CTGCAG	1 cut(s)	190
PstNI	CAGNNNCTG	2 cut(s)	2099, 2300
PsuI	RGATCY	3 cut(s)	668, 2425, 2629
PvuII	CAGCTG	2 cut(s)	327, 2714
RsaI	GTAC	3 cut(s)	716, 782, 1157
RsaNI	GTAC	3 cut(s)	715, 781, 1156
RseI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
SapI	GCTCTTC	3 cut(s)	357, 1934, 2591
SaqAI	TTAA	6 cut(s)	78, 213, 228, 917, 1314, 2403
Sau96I	GGNCC	4 cut(s)	662, 2180, 2676, 2677
ScaI	AGTACT	1 cut(s)	1157
SchI	GAGTC	5 cut(s)	344, 1882, 2145, 2501, 2727
ScrFI	CCNGG	6 cut(s)	719, 1326, 1647, 1667, 2174, 2681
SduI	GDGCHC	6 cut(s)	210, 1034, 1555, 1929, 2091, 2680
SfaNI	GCATC	7 cut(s)	110, 457, 840, 900, 1180, 1454, 2352
SfcI	CTRYAG	5 cut(s)	186, 237, 1773, 2370, 2568
SfuI	TTCGAA	1 cut(s)	355
SinI	GGWCC	2 cut(s)	662, 2180
SmiMI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
SmlI	CTYRAG	1 cut(s)	1313
SmoI	CTYRAG	1 cut(s)	1313
SpeI	ACTAGT	2 cut(s)	440, 1118
SphI	GCATGC	3 cut(s)	321, 2024, 2704
SsiI	CCGC	6 cut(s)	760, 2158, 2261, 2305, 2477, 2577
SspMI	CTAG	5 cut(s)	392, 441, 1119, 1568, 1763
StyD4I	CCNGG	6 cut(s)	717, 1324, 1645, 1665, 2172, 2679
StyI	CCWWGG	3 cut(s)	708, 1755, 2753
TaaI	ACNGT	6 cut(s)	70, 163, 904, 1603, 1867, 1897
TaiI	ACGT	4 cut(s)	682, 997, 2045, 2565
TatI	WGTACW	2 cut(s)	780, 1155
TauI	GCSGC	2 cut(s)	2161, 2579
Tru1I	TTAA	6 cut(s)	78, 213, 228, 917, 1314, 2403
Tru9I	TTAA	6 cut(s)	78, 213, 228, 917, 1314, 2403
TseFI	GTSAC	4 cut(s)	631, 1364, 1427, 2649
Tsp45I	GTSAC	4 cut(s)	631, 1364, 1427, 2649
TspDTI	ATGAA	8 cut(s)	509, 937, 1466, 1630, 2345, 2480, 2609, 2772
TspGWI	ACGGA	2 cut(s)	565, 2438
Vha464I	CTTAAG	1 cut(s)	1313
VneI	GTGCAC	1 cut(s)	2087
VpaK11BI	GGWCC	2 cut(s)	662, 2180
VspI	ATTAAT	2 cut(s)	213, 2403
XapI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2655, 2739
XceI	RCATGY	6 cut(s)	321, 2024, 2063, 2232, 2704, 2772
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1942
XmnI	GAANNNNTTC	1 cut(s)	541
XspI	CTAG	5 cut(s)	392, 441, 1119, 1568, 1763
ZrmI	AGTACT	1 cut(s)	1157
Zsp2I	ATGCAT	1 cut(s)	988

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.