Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

56447031 .. 56454233

Gene Structure 7203 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G033260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2892 bp

ATGGATTCATACCATTTGAATGGCGGAGTGGATGCTAACGGGATGGATGATATCAACCGGCCTCTGTTGGAGCCCTTGGATGTTAGGGCCGCAGATAAGAGAATTAGAACACTTAAGTTCAAAATTGGGGAAATCCACTGTGCTTCTTGTTCAACTACCATTGAATCTGTGGTGGGAAGCCTTGATGGGGTCAAGAGCGTTACGGTGTCACCCATTCATGGCCAAGCTGCTATCGATTATATACCAGAGCTCATTAATGGAAGCAAAATCAAGGAAGCCATAGAAGATGCAGGTTTCCCAGTTGATGAATTTCCAGAACAAGACATTGCAGTATGCCGGCTCAGGATAAAAGGAATGATGTGCACCAGTTGCTCCGAGTCCGTTGAATCTGCTCTTCGAATGGTTGATGGAGTGAAAAACGCAGTTGTTGGTCTAGCCCTTGAGGAAGCAAAGGTTCACTTTGATCCAAACATCACTGACACCTGTTCGATCATGAAAGCAATAGAAGATGCTGGCTTTGGATCTGAGCTCATTAGCTCAGGGAATGATGTAAACAAAGTGCATCTAAGACTTGAAGGAGTTAATTCTCCTGAAGACATGACCATCATCCAATCCTCCCTTGAATCAGTTGAGGGTGTAAATAATGTTGAAGTGGACGTGTTAGAAAAAAAGGTCACCCTCACCTACGATGCAGACCTCACTGGTCCAAGATCTCTTATACATTGTATTGAAGAAGCTGGATACCAAGCAAGCTTGTATGTTCCTCCAAGGCGAAGAGAAGCTGAACAGCAGCTTGAGACTCAGATGTACAGGAACCAGTTTTTCTTGAGCTGTCTATTTTCAGTTCCTGTATTTTTATTCTCAATGGTGCTTCCAATGCTTTCTCCCTATGGGGACTGGTTAATGTACAAGATTCACAACATGCTCACTGTAGGATTGCTTTTAAGATGGATTCTGTGCACACCCGTGCAGTTCATTATTGGCAGGAGGTTTTATGTGGGATCATATCATGCTTTAAGACGTAGGTCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTCTACTCTGTCTATATAGCAATGAAAGCATTGGCTTTGGATAATTTTGAGGGGGAAGATTTCTTTGAAACTAGTTCCATGATGATATCCTTTATACTCTTGGGGAAGTACTTAGAAGTTCTAGCTAGAGGGAAAACATCTGATGCTTTAGCAAAGCTGACAGACCTTACTCCTGAAACTGCTTATCTGCTATCACTAGATGCTGATGGCAATGCTATCTCAGAGATGGAAATTAGCACTCAACTTATTCAAAGAAATGATATACTTAAGATTGTTCCCGGGGCAAAAGTTCCTGTTGATGGGATTGTAATTAGTGGTCAAAGCCATGTGAACGAGAGTATGATCACAGGAGAGGCAAGGCCTATTTCTAAAAGATTAGGTGACAAGGTAATTGGTGGCACCATGAATGAGAATGGATGCTTACAGGTTAAGGCAACCCATGTTGGTTCAGAGACTGCGCTATCCCAGATAGTTCAACTTGTTGAAGCTGCACAGCTTTCCAGAGCACCTGTTCAGAAGCTAGCTGATCAGATCTCAAAGTTTTTTGTTCCAGCAGTTGTGATTGCAGCATTTCTGACATGGCTGGGTTGGTTCATCCTGGGAGAATTCGGTCTTTATCCCATATCATGGACACCAAAAGGCATGGATAGGTTTGAGCTGGCACTGCAGTTCGGCATCTCAGTACTAGTGGTTGCATGCCCTTGTGCTTTGGGATTAGCAACACCTACAGCAGTCATGGTTGCAACAGGGAAGGGTGCTACACAAGGTGTTCTTATCAAGGGTGGAAATGCACTTGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAGACAGGGACACTGACAGTTGGAAAACCGACAGTAGTTAGTGCTGTTCTATTTTCCAATTACTCAATGGAGGAATTCTGTACTGTGGCTACTGCTGCAGAGGCAAATAGTGAACACCCAATAGCAAAGTCTATTGTGGAGCATGCAAAGGGGTTTCTTAATAAGTTTGGATCAACTGAACATGTGGTAGAGGCTAAGGACTTTGAGGTACACACTGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGGCTCATGCGGGAATATAATGTCCAGGTTGGTCCTGAGGTTGAGAAATTCGTTTCAGAAAATGAGAAACTGGCTCGAACATGTGTGTTAGTTTCCATTGATGGAAAGGTTGCTGGGTCGTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCATGTGTAATATCTTATCTCCACTCAATGGGCATTTCAAGCATCATGGTTACTGGTGATAATTTGGCAACAGCAGCAGCAATTGCAAGGGAGGTTGGCATCGATAAGGTGTTTGCTGAGACCGATCCCATGGGAAAAGCTGATAGAATCAAAGAGTTACAGATGAAAGGATTGACTGTGACTATGGTGGGAGATGGAATCAATGACTCACCAGCTTTGGCTGCAGCAGATGTTGGCATGGCAATTGGCGCTGGCACCGATGTAGCAATTGAAGCTGCTGATATAGTTTTGATGAAAAGCAATTTGGAAGATGTAGTTACAGCCATAGATCTCTCTAGAAAGACTATGTCTCGAATTTGGTTGAATTATGTCTGGGCCCTGGGATACAACATCCTTGGCATGCCAGTTGCTGCGGGAATCTTGTTCCCTTTCACTGGGATCCGATTACCACCTTGGCTTGCCGGTGCCTGTATGGCTGCTTCATCTGTTAGTGTGGTATGTTCATCTCTCTTGTTGCAGTCTTATAAGAAGCCTTTGCATTTTCAGAACATTAAGTCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

963

Amino Acids

103.99

Weight (kDa)

5.38

Isoelectric Point (pI)

36.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	40 - 99	2.7e-12	Heavy-metal-associated domain
HMA	PF00403	114 - 173	8.8e-09	Heavy-metal-associated domain
HMA	PF00403	203 - 248	2.8e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	416 - 520	7.7e-25	P-type ATPase actuator domain
Hydrolase	PF00702	629 - 853	1e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2853
Acc36I	ACCTGC	1 cut(s)	281
AccB1I	GGYRCC	4 cut(s)	1058, 1472, 2582, 2792
AccB7I	CCANNNNNTGG	2 cut(s)	1701, 2356
AciI	CCGC	4 cut(s)	24, 90, 2185, 2741
AclWI	GGATC	8 cut(s)	458, 529, 1009, 2083, 2303, 2447, 2761, 2774
AcoI	YGGCCR	1 cut(s)	220
AcsI	RAATTY	5 cut(s)	308, 1679, 1979, 2222, 2682
AcuI	CTGAAG	1 cut(s)	612
AdeI	CACNNNGTG	1 cut(s)	1841
AfaI	GTAC	6 cut(s)	809, 908, 1184, 1758, 1987, 2115
AfiI	CCNNNNNNNGG	6 cut(s)	187, 218, 1373, 1701, 2356, 2762
AflII	CTTAAG	2 cut(s)	113, 1340
AflIII	ACRYGT	3 cut(s)	657, 2086, 2255
AhlI	ACTAGT	2 cut(s)	1145, 1759
AjiI	CACGTC	1 cut(s)	658
AjnI	CCWGG	3 cut(s)	1671, 2199, 2706
AjuI	GAANNNNNNNTTGG	2 cut(s)	2615, 2647
AleI	CACNNNNGTG	1 cut(s)	965
Alw21I	GWGCWC	5 cut(s)	252, 365, 531, 962, 1582
Alw26I	GTCTC	4 cut(s)	791, 1520, 2441, 2682
Alw44I	GTGCAC	2 cut(s)	361, 958
AlwI	GGATC	8 cut(s)	458, 529, 1009, 2083, 2303, 2447, 2761, 2774
AlwNI	CAGNNNCTG	4 cut(s)	848, 1529, 2327, 2738
Ama87I	CYCGRG	1 cut(s)	1352
AoxI	GGCC	5 cut(s)	59, 87, 220, 1433, 2703
ApaI	GGGCCC	1 cut(s)	2707
ApaLI	GTGCAC	2 cut(s)	361, 958
ApoI	RAATTY	5 cut(s)	308, 1679, 1979, 2222, 2682
AseI	ATTAAT	1 cut(s)	255
AspLEI	GCGC	2 cut(s)	1534, 2579
AspS9I	GGNCC	5 cut(s)	87, 704, 2207, 2703, 2704
AsuC2I	CCSGG	2 cut(s)	1353, 1354
AsuHPI	GGTGA	8 cut(s)	201, 667, 673, 1466, 1897, 2159, 2396, 2529
AsuII	TTCGAA	1 cut(s)	397
AsuNHI	GCTAGC	1 cut(s)	1594
AvaI	CYCGRG	1 cut(s)	1352
AvaII	GGWCC	2 cut(s)	704, 2207
AxyI	CCTNAGG	1 cut(s)	2211
BaeGI	GKGCMC	4 cut(s)	365, 962, 1061, 2707
BalI	TGGCCA	1 cut(s)	222
BamHI	GGATCC	1 cut(s)	2766
BanI	GGYRCC	4 cut(s)	1058, 1472, 2582, 2792
BanII	GRGCYC	4 cut(s)	75, 252, 531, 2707
BarI	GAAGNNNNNNTAC	2 cut(s)	2791, 2823
BbsI	GAAGAC	1 cut(s)	600
Bbv12I	GWGCWC	5 cut(s)	252, 365, 531, 962, 1582
BciT130I	CCWGG	3 cut(s)	1673, 2201, 2708
BciVI	GTATCC	2 cut(s)	734, 2705
BclI	TGATCA	2 cut(s)	1416, 1600
BcnI	CCSGG	2 cut(s)	1353, 1354
BcoDI	GTCTC	4 cut(s)	791, 1520, 2441, 2682
BcuI	ACTAGT	2 cut(s)	1145, 1759
BfaI	CTAG	8 cut(s)	434, 1146, 1196, 1200, 1271, 1595, 1760, 2664
BfmI	CTRYAG	5 cut(s)	930, 1739, 1800, 2001, 2550
BfoI	RGCGCY	1 cut(s)	2580
BfrI	CTTAAG	2 cut(s)	113, 1340
BfuAI	ACCTGC	1 cut(s)	281
BfuI	GTATCC	2 cut(s)	734, 2705
BglI	GCCNNNNNGGC	1 cut(s)	2801
BglII	AGATCT	3 cut(s)	710, 1605, 2656
BmcAI	AGTACT	2 cut(s)	1184, 1758
Bme1390I	CCNGG	5 cut(s)	1353, 1354, 1673, 2201, 2708
Bme18I	GGWCC	2 cut(s)	704, 2207
BmeT110I	CYCGRG	1 cut(s)	1352
BmgBI	CACGTC	1 cut(s)	658
BmgT120I	GGNCC	5 cut(s)	87, 704, 2207, 2703, 2704
BmiI	GGNNCC	8 cut(s)	72, 815, 1060, 1474, 2584, 2705, 2768, 2794
BmrFI	CCNGG	5 cut(s)	1353, 1354, 1673, 2201, 2708
BmrI	ACTGGG	3 cut(s)	293, 1064, 2772
BmtI	GCTAGC	1 cut(s)	1598
BmuI	ACTGGG	3 cut(s)	293, 1064, 2772
BoxI	GACNNNNGTC	1 cut(s)	1024
BpiI	GAAGAC	1 cut(s)	600
BpmI	CTGGAG	1 cut(s)	2142
Bpu10I	CCTNAGC	3 cut(s)	341, 538, 2100
Bpu14I	TTCGAA	1 cut(s)	397
BpuEI	CTTGAG	3 cut(s)	461, 815, 847
BpuMI	CCSGG	2 cut(s)	1353, 1354
Bsa29I	ATCGAT	2 cut(s)	234, 2430
BsaI	GGTCTC	1 cut(s)	2441
BsaXI	ACNNNNNCTCC	2 cut(s)	2115, 2145
Bsc4I	CCNNNNNNNGG	6 cut(s)	187, 218, 1373, 1701, 2356, 2762
Bse118I	RCCGGY	3 cut(s)	57, 336, 2789
Bse21I	CCTNAGG	1 cut(s)	2211
Bse3DI	GCAATG	3 cut(s)	324, 1101, 1291
BseBI	CCWGG	3 cut(s)	1673, 2201, 2708
BseCI	ATCGAT	2 cut(s)	234, 2430
BseGI	GGATG	9 cut(s)	37, 48, 52, 85, 606, 1045, 1496, 1668, 2718
BseLI	CCNNNNNNNGG	6 cut(s)	187, 218, 1373, 1701, 2356, 2762
BseMI	GCAATG	3 cut(s)	324, 1101, 1291
BseMII	CTCAG	8 cut(s)	355, 516, 552, 815, 1308, 1767, 2202, 2436
BseSI	GKGCMC	4 cut(s)	365, 962, 1061, 2707
BseYI	CCCAGC	2 cut(s)	1657, 2288
BsgI	GTGCAG	2 cut(s)	989, 1548
BshFI	GGCC	5 cut(s)	61, 89, 222, 1435, 2705
BshNI	GGYRCC	4 cut(s)	1058, 1472, 2582, 2792
BshVI	ATCGAT	2 cut(s)	234, 2430
BsiHKAI	GWGCWC	5 cut(s)	252, 365, 531, 962, 1582
BsiHKCI	CYCGRG	1 cut(s)	1352
BsiSI	CCGG	4 cut(s)	58, 337, 1353, 2790
BslFI	GGGAC	2 cut(s)	908, 1927
BslI	CCNNNNNNNGG	6 cut(s)	187, 218, 1373, 1701, 2356, 2762
BsmAI	GTCTC	4 cut(s)	791, 1520, 2441, 2682
BsmFI	GGGAC	2 cut(s)	908, 1927
BsnI	GGCC	5 cut(s)	61, 89, 222, 1435, 2705
Bso31I	GGTCTC	1 cut(s)	2441
BsoBI	CYCGRG	1 cut(s)	1352
Bsp119I	TTCGAA	1 cut(s)	397
Bsp120I	GGGCCC	1 cut(s)	2703
Bsp1286I	GDGCHC	8 cut(s)	75, 252, 365, 531, 962, 1061, 1582, 2707
Bsp1407I	TGTACA	2 cut(s)	807, 906
Bsp19I	CCATGG	1 cut(s)	2457
BspACI	CCGC	4 cut(s)	24, 90, 2185, 2741
BspANI	GGCC	5 cut(s)	61, 89, 222, 1435, 2705
BspCNI	CTCAG	8 cut(s)	354, 517, 551, 814, 1307, 1766, 2203, 2437
BspDI	ATCGAT	2 cut(s)	234, 2430
BspHI	TCATGA	1 cut(s)	492
BspLI	GGNNCC	8 cut(s)	72, 815, 1060, 1474, 2584, 2705, 2768, 2794
BspMAI	CTGCAG	3 cut(s)	1743, 2005, 2554
BspMI	ACCTGC	1 cut(s)	281
BspOI	GCTAGC	1 cut(s)	1598
BspPI	GGATC	8 cut(s)	458, 529, 1009, 2083, 2303, 2447, 2761, 2774
BspQI	GCTCTTC	1 cut(s)	399
BspT104I	TTCGAA	1 cut(s)	397
BspT107I	GGYRCC	4 cut(s)	1058, 1472, 2582, 2792
BspTI	CTTAAG	2 cut(s)	113, 1340
BspTNI	GGTCTC	1 cut(s)	2441
BsrDI	GCAATG	3 cut(s)	324, 1101, 1291
BsrFI	RCCGGY	3 cut(s)	57, 336, 2789
BsrGI	TGTACA	2 cut(s)	807, 906
BssAI	RCCGGY	3 cut(s)	57, 336, 2789
BssT1I	CCWWGG	5 cut(s)	75, 767, 2457, 2722, 2780
Bst2UI	CCWGG	3 cut(s)	1673, 2201, 2708
Bst4CI	ACNGT	8 cut(s)	140, 205, 931, 1894, 1924, 1939, 1990, 2506
Bst6I	CTCTTC	3 cut(s)	399, 769, 2134
BstAFI	CTTAAG	2 cut(s)	113, 1340
BstAPI	GCANNNNNTGC	2 cut(s)	369, 2411
BstAUI	TGTACA	2 cut(s)	807, 906
BstBI	TTCGAA	1 cut(s)	397
BstDSI	CCRYGG	1 cut(s)	2457
BstEII	GGTNACC	1 cut(s)	673
BstF5I	GGATG	9 cut(s)	37, 48, 52, 85, 606, 1045, 1496, 1668, 2718
BstH2I	RGCGCY	1 cut(s)	2580
BstHHI	GCGC	2 cut(s)	1534, 2579
BstMAI	GTCTC	4 cut(s)	791, 1520, 2441, 2682
BstNI	CCWGG	3 cut(s)	1673, 2201, 2708
BstNSI	RCATGY	7 cut(s)	925, 1773, 2051, 2090, 2259, 2334, 2731
BstPAI	GACNNNNGTC	1 cut(s)	1024
BstPI	GGTNACC	1 cut(s)	673
BstSCI	CCNGG	5 cut(s)	1351, 1352, 1671, 2199, 2706
BstSFI	CTRYAG	5 cut(s)	930, 1739, 1800, 2001, 2550
BstSLI	GKGCMC	4 cut(s)	365, 962, 1061, 2707
BstV2I	GAAGAC	1 cut(s)	600
BstX2I	RGATCY	5 cut(s)	521, 710, 1605, 2656, 2766
BstXI	CCANNNNNNTGG	1 cut(s)	20
BstYI	RGATCY	5 cut(s)	521, 710, 1605, 2656, 2766
Bsu15I	ATCGAT	2 cut(s)	234, 2430
Bsu36I	CCTNAGG	1 cut(s)	2211
BsuI	GTATCC	2 cut(s)	734, 2705
BsuRI	GGCC	5 cut(s)	61, 89, 222, 1435, 2705
BsuTUI	ATCGAT	2 cut(s)	234, 2430
BtgI	CCRYGG	1 cut(s)	2457
BtrI	CACGTC	1 cut(s)	658
BtsCI	GGATG	9 cut(s)	37, 48, 52, 85, 606, 1045, 1496, 1668, 2718
BtsI	GCAGTG	1 cut(s)	1736
BveI	ACCTGC	1 cut(s)	281
CaiI	CAGNNNCTG	4 cut(s)	848, 1529, 2327, 2738
CciI	TCATGA	1 cut(s)	492
CfoI	GCGC	2 cut(s)	1534, 2579
Cfr10I	RCCGGY	3 cut(s)	57, 336, 2789
Cfr13I	GGNCC	5 cut(s)	87, 704, 2207, 2703, 2704
Cfr9I	CCCGGG	1 cut(s)	1352
ClaI	ATCGAT	2 cut(s)	234, 2430
Csp6I	GTAC	6 cut(s)	808, 907, 1183, 1757, 1986, 2114
CviQI	GTAC	6 cut(s)	808, 907, 1183, 1757, 1986, 2114
DraIII	CACNNNGTG	1 cut(s)	1841
EaeI	YGGCCR	1 cut(s)	220
Eam1104I	CTCTTC	3 cut(s)	399, 769, 2134
EarI	CTCTTC	3 cut(s)	399, 769, 2134
EciI	GGCGGA	1 cut(s)	39
Ecl136II	GAGCTC	2 cut(s)	250, 529
Eco130I	CCWWGG	5 cut(s)	75, 767, 2457, 2722, 2780
Eco147I	AGGCCT	1 cut(s)	1435
Eco24I	GRGCYC	4 cut(s)	75, 252, 531, 2707
Eco31I	GGTCTC	1 cut(s)	2441
Eco32I	GATATC	2 cut(s)	52, 1161
Eco47I	GGWCC	2 cut(s)	704, 2207
Eco53kI	GAGCTC	2 cut(s)	250, 529
Eco57I	CTGAAG	1 cut(s)	612
Eco81I	CCTNAGG	1 cut(s)	2211
Eco88I	CYCGRG	1 cut(s)	1352
Eco91I	GGTNACC	1 cut(s)	673
EcoICRI	GAGCTC	2 cut(s)	250, 529
EcoO109I	RGGNCCY	1 cut(s)	2704
EcoO65I	GGTNACC	1 cut(s)	673
EcoRI	GAATTC	2 cut(s)	1679, 1979
EcoRII	CCWGG	3 cut(s)	1671, 2199, 2706
EcoRV	GATATC	2 cut(s)	52, 1161
EcoT14I	CCWWGG	5 cut(s)	75, 767, 2457, 2722, 2780
EcoT38I	GRGCYC	4 cut(s)	75, 252, 531, 2707
ErhI	CCWWGG	5 cut(s)	75, 767, 2457, 2722, 2780
FalI	AAGNNNNNCTT	4 cut(s)	443, 475, 1830, 1862
FaqI	GGGAC	2 cut(s)	908, 1927
FauI	CCCGC	2 cut(s)	2178, 2734
FbaI	TGATCA	2 cut(s)	1416, 1600
FokI	GGATG	9 cut(s)	44, 55, 59, 92, 593, 1052, 1503, 1655, 2705
FriOI	GRGCYC	4 cut(s)	75, 252, 531, 2707
FspBI	CTAG	8 cut(s)	434, 1146, 1196, 1200, 1271, 1595, 1760, 2664
GlaI	GCGC	2 cut(s)	1533, 2578
GsaI	CCCAGC	2 cut(s)	1661, 2292
GsuI	CTGGAG	1 cut(s)	2142
HaeII	RGCGCY	1 cut(s)	2580
HaeIII	GGCC	5 cut(s)	61, 89, 222, 1435, 2705
HapII	CCGG	4 cut(s)	58, 337, 1353, 2790
HhaI	GCGC	2 cut(s)	1534, 2579
Hin6I	GCGC	2 cut(s)	1532, 2577
HinP1I	GCGC	2 cut(s)	1532, 2577
HindIII	AAGCTT	1 cut(s)	751
HpaII	CCGG	4 cut(s)	58, 337, 1353, 2790
HphI	GGTGA	8 cut(s)	201, 667, 673, 1466, 1897, 2159, 2396, 2529
Hpy166II	GTNNAC	8 cut(s)	363, 457, 553, 655, 960, 1405, 2018, 2116
Hpy8I	GTNNAC	8 cut(s)	363, 457, 553, 655, 960, 1405, 2018, 2116
HpyAV	CCTTC	2 cut(s)	569, 1819
HpyCH4III	ACNGT	8 cut(s)	140, 205, 931, 1894, 1924, 1939, 1990, 2506
HpyCH4IV	ACGT	2 cut(s)	657, 1021
HpySE526I	ACGT	2 cut(s)	657, 1021
HspAI	GCGC	2 cut(s)	1532, 2577
KroI	GCCGGC	1 cut(s)	336
KroNI	GCCGGC	1 cut(s)	338
Ksp22I	TGATCA	2 cut(s)	1416, 1600
LguI	GCTCTTC	1 cut(s)	399
LmnI	GCTCC	4 cut(s)	70, 377, 2044, 2123
MaeI	CTAG	8 cut(s)	434, 1146, 1196, 1200, 1271, 1595, 1760, 2664
MaeII	ACGT	2 cut(s)	657, 1021
MboII	GAAGA	9 cut(s)	296, 386, 518, 605, 743, 786, 1142, 2151, 2648
MfeI	CAATTG	3 cut(s)	2409, 2571, 2595
MflI	RGATCY	5 cut(s)	521, 710, 1605, 2656, 2766
MhlI	GDGCHC	8 cut(s)	75, 252, 365, 531, 962, 1061, 1582, 2707
MlsI	TGGCCA	1 cut(s)	222
MluNI	TGGCCA	1 cut(s)	222
MlyI	GAGTC	3 cut(s)	386, 793, 2528
MmeI	TCCRAC	2 cut(s)	48, 1906
Mox20I	TGGCCA	1 cut(s)	222
MroNI	GCCGGC	1 cut(s)	336
MscI	TGGCCA	1 cut(s)	222
MslI	CAYNNNNRTG	2 cut(s)	18, 965
Msp20I	TGGCCA	1 cut(s)	222
MspCI	CTTAAG	2 cut(s)	113, 1340
MspI	CCGG	4 cut(s)	58, 337, 1353, 2790
MspR9I	CCNGG	5 cut(s)	1353, 1354, 1673, 2201, 2708
MunI	CAATTG	3 cut(s)	2409, 2571, 2595
MvaI	CCWGG	3 cut(s)	1673, 2201, 2708
NaeI	GCCGGC	1 cut(s)	338
NciI	CCSGG	2 cut(s)	1353, 1354
NcoI	CCATGG	1 cut(s)	2457
NgoMIV	GCCGGC	1 cut(s)	336
NheI	GCTAGC	1 cut(s)	1594
NlaIV	GGNNCC	8 cut(s)	72, 815, 1060, 1474, 2584, 2705, 2768, 2794
NmuCI	GTSAC	5 cut(s)	207, 673, 1454, 2147, 2506
NspI	RCATGY	7 cut(s)	925, 1773, 2051, 2090, 2259, 2334, 2731
NspV	TTCGAA	1 cut(s)	397
OliI	CACNNNNGTG	1 cut(s)	965
PaeI	GCATGC	3 cut(s)	1773, 2051, 2731
PagI	TCATGA	1 cut(s)	492
PasI	CCCWGGG	1 cut(s)	2707
PceI	AGGCCT	1 cut(s)	1435
PciI	ACATGT	2 cut(s)	2086, 2255
PciSI	GCTCTTC	1 cut(s)	399
PdiI	GCCGGC	1 cut(s)	338
PfeI	GAWTC	9 cut(s)	5, 164, 386, 623, 913, 952, 2475, 2526, 2745
PflFI	GACNNNGTC	1 cut(s)	2674
PflMI	CCANNNNNTGG	2 cut(s)	1701, 2356
PleI	GAGTC	3 cut(s)	385, 793, 2528
PpsI	GAGTC	3 cut(s)	385, 793, 2528
PscI	ACATGT	2 cut(s)	2086, 2255
PshAI	GACNNNNGTC	1 cut(s)	1024
PshBI	ATTAAT	1 cut(s)	255
PsiI	TTATAA	1 cut(s)	2853
Psp124BI	GAGCTC	2 cut(s)	252, 531
Psp6I	CCWGG	3 cut(s)	1671, 2199, 2706
PspEI	GGTNACC	1 cut(s)	673
PspFI	CCCAGC	2 cut(s)	1657, 2288
PspGI	CCWGG	3 cut(s)	1671, 2199, 2706
PspN4I	GGNNCC	8 cut(s)	72, 815, 1060, 1474, 2584, 2705, 2768, 2794
PspOMI	GGGCCC	1 cut(s)	2703
PspPI	GGNCC	5 cut(s)	87, 704, 2207, 2703, 2704
PstI	CTGCAG	3 cut(s)	1743, 2005, 2554
PstNI	CAGNNNCTG	4 cut(s)	848, 1529, 2327, 2738
PsuI	RGATCY	5 cut(s)	521, 710, 1605, 2656, 2766
PsyI	GACNNNGTC	1 cut(s)	2674
RsaI	GTAC	6 cut(s)	809, 908, 1184, 1758, 1987, 2115
RsaNI	GTAC	6 cut(s)	808, 907, 1183, 1757, 1986, 2114
RseI	CAYNNNNRTG	2 cut(s)	18, 965
SacI	GAGCTC	2 cut(s)	252, 531
SapI	GCTCTTC	1 cut(s)	399
Sau96I	GGNCC	5 cut(s)	87, 704, 2207, 2703, 2704
ScaI	AGTACT	2 cut(s)	1184, 1758
SchI	GAGTC	3 cut(s)	386, 793, 2528
ScrFI	CCNGG	5 cut(s)	1353, 1354, 1673, 2201, 2708
SduI	GDGCHC	8 cut(s)	75, 252, 365, 531, 962, 1061, 1582, 2707
SfcI	CTRYAG	5 cut(s)	930, 1739, 1800, 2001, 2550
SfuI	TTCGAA	1 cut(s)	397
SinI	GGWCC	2 cut(s)	704, 2207
SmaI	CCCGGG	1 cut(s)	1354
SmiMI	CAYNNNNRTG	2 cut(s)	18, 965
SmlI	CTYRAG	5 cut(s)	113, 440, 794, 826, 1340
SmoI	CTYRAG	5 cut(s)	113, 440, 794, 826, 1340
SpeI	ACTAGT	2 cut(s)	1145, 1759
SphI	GCATGC	3 cut(s)	1773, 2051, 2731
SseBI	AGGCCT	1 cut(s)	1435
SsiI	CCGC	4 cut(s)	24, 90, 2185, 2741
SspMI	CTAG	8 cut(s)	434, 1146, 1196, 1200, 1271, 1595, 1760, 2664
SstI	GAGCTC	2 cut(s)	252, 531
StuI	AGGCCT	1 cut(s)	1435
StyD4I	CCNGG	5 cut(s)	1351, 1352, 1671, 2199, 2706
StyI	CCWWGG	5 cut(s)	75, 767, 2457, 2722, 2780
TaaI	ACNGT	8 cut(s)	140, 205, 931, 1894, 1924, 1939, 1990, 2506
TaiI	ACGT	2 cut(s)	660, 1024
TaqI	TCGA	6 cut(s)	234, 397, 488, 2251, 2430, 2680
TaqII	GACCGA	2 cut(s)	1673, 2465
TatI	WGTACW	5 cut(s)	807, 906, 1182, 1756, 1985
TauI	GCSGC	1 cut(s)	92
TfiI	GAWTC	9 cut(s)	5, 164, 386, 623, 913, 952, 2475, 2526, 2745
TseFI	GTSAC	5 cut(s)	207, 673, 1454, 2147, 2506
Tsp45I	GTSAC	5 cut(s)	207, 673, 1454, 2147, 2506
TspGWI	ACGGA	1 cut(s)	370
TspMI	CCCGGG	1 cut(s)	1352
Tth111I	GACNNNGTC	1 cut(s)	2674
Van91I	CCANNNNNTGG	2 cut(s)	1701, 2356
Vha464I	CTTAAG	2 cut(s)	113, 1340
VneI	GTGCAC	2 cut(s)	361, 958
VpaK11BI	GGWCC	2 cut(s)	704, 2207
VspI	ATTAAT	1 cut(s)	255
XapI	RAATTY	5 cut(s)	308, 1679, 1979, 2222, 2682
XbaI	TCTAGA	1 cut(s)	2663
XceI	RCATGY	7 cut(s)	925, 1773, 2051, 2090, 2259, 2334, 2731
XcmI	CCANNNNNNNNNTGG	2 cut(s)	166, 1969
XmaI	CCCGGG	1 cut(s)	1352
XspI	CTAG	8 cut(s)	434, 1146, 1196, 1200, 1271, 1595, 1760, 2664
ZrmI	AGTACT	2 cut(s)	1184, 1758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.