Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

57012971 .. 57092069

Gene Structure 79099 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG363900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2889 bp

ATGGATTCAAACCATTTGAATGGCGGAGTGGATGCTAACGGGATGGATGATATCAACCGGCCTCTGTTGGAGCCCTTGGATGTTAGAGCCGCAGATAAGAGAATTAGAACACTTAAGTTCAAAATTGGGGAAATCCACTGTGCTTCTTGTTCAACCACCATTGAATCTGTGGTGGGAAGCCTTAATGGGGTCAAGAGCGTTACGGTGTCACCCATTCATGGCCAAGCTGCTATCGATTATATACCAGAGCTCATTAATGGAAGCAAAATCAAGGAAGCCATAGAAGATGCAGGTTTCCCAGTTGATGAATTTCCAGAACAAGACATTGCAGTATGCCGGCTCAGGATAAAAGGAATGATGTGCACCAGTTGCTCCGAGTCCGTTGAATCTGCTCTTCGAATGGTTGATGGAGTGAAAAACGCAGTTGTTGGTCTAGCCCTTGAGGAAGCAAAGGTTCACTTTGATCCAAACATCACTGACACCGGTTCGATCATGAAAGCAATAGAAGATGCTGGCTTTGGATCTGAGCTCATTAGCTCAGGGAATGATGTAAACAAAGTGCATCTAAAACTTGAAGGAGTTAATTCTCCTGAAGACATGACCATCATCCAATCCTCCCTTGAATCAGTTGAGGGTGTAAATAATGTTGAAGTGGACGTGTTAGAAAAAAAGGTCACCATCACCTACGATGCAGACCTCACTGGTCCAAGATCTCTTATACATTGTATTGAAGAAGCTGGAAGTAACCCCAAATCATACCAAGCAAGCTTGTATGTTCCTCCAAGGCGAAGAGAAGCTGAACAGCAGCTTGAGACTCAGATGTACAGGAACCAGTTTTTCTTGAGCTGTCTATTTTCAGTTCCGGTATTTTTATTCTCAATGGTGCTTCCAATGCTTTCTCCCTATGGGGACTGGTTAATGTACAAGATTCACAACATGCTCACTGTAGGAATGCTTTTAAGATGGATTCTGTGCACACCCGTGCAGTTCATTATTGGCAGGAGGTTTTATGTGGGATCATATCATGCTTTAAGACGTAGGTCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTCTACTCTGTCTATATAGCAATGAAAGCATTGGCTTTGGATAATTTTGAGGGGGAAGATTTCTTTGAAACTAGTTCCATGATGATATCCTTTATACTCCTGGGGAAGTACTTAGAAGTTCTAGCTAGAGGAAAAACATCTGATGCTTTAGCAAAGCTGACAGACCTTACTCCTGAAACGGCTTATCTGCTATCACTAGATGCTGATGGCAATGCTATCTCAGAGATGGAAATTAGCACTCAACTTATTCAAAGAAATGATATACTTAAGATTGTTCCCGGGGCAAAAGTTCCTGTTGATGGGATTGTAATTAGTGGTCAAAGCCATGTGAACGAGAGTATGATCACAGGAGAGGCAAGGCCTATTTCTAAAAGATTAGGTGACAAGGTAATTGGTGGCACCATGAATGAGAATGGATGCTTACAGGTTAAGGCAACCCATGTTGGTTCAGAGACTGCGCTATCCCAGATAGTTCAACTTGTTGAAGCTGCACAGCTTTCCAGAGCACCTGTTCAGAAGCTAGCTGATCAGATCTCAAAGTTTTTCGTTCCAGCAGTTGTGATTGCAGCATTTCTGACATGGCTGGGTTGGTTCATCCTGGGAGAATTCGGTCTTTATCCCATATCATGGACACCAAAAGGCATGGATAGGTTTGAGCTGGCACTGCAGTTCGGCATCTCAGTACTAGTGGTTGCATGCCCTTGTGCTTTGGGATTAGCAACACCTACAGCAGTCATGGTTGCAACAGGGAAGGGTGCTACACAAGGTGTTCTTATCAAGGGTGGAAATGCACTTGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAGACAGGGACACTGACAGTTGGAAAACCGACAGTAGTTAGTGCTGTTCTATTTTCCAATTACTCAATGGAGGAATTCTGTACTGTGGCTACTGCTGCAGAGGCAAATAGTGAACACCCAATAGCAAAGTCTATTGTGGAGCATGCAAAGGGGTTTCTTAACAAGTTTGGGTCAACTGAACATGTGGTGGAGGCTAAGGACTTTGAGGTACACACTGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGGCTCATGCGGGAATATAATGTCCAGGTTGGTCCTGAGGTTGAGAAATTCGTTTCAGAAAATGAGAAACTGGCTCGAACATGTGTGTTAGTTTCCATTGATGGAAAGGTTGCTGGGTCGTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCATGTGTAATATCTTATCTCCACTCAATGGGCATTTCAAGCATCATGGTTACTGGTGATAATCAAAAAGTTGGCATCGATATCGATAAGGTGTTTGCTGAGACCGATCCCATGGGAAAAGCTGATAGAATCAAAAAGTTACAGATGAAAGGATTGACTGTGACTATGGTGGGAGATGGAATCAATGACTCACCAGCTTTGGCTGCAGCAGATGTTGGCATGGCAATTGGCGCTGGCACCAATGTAGCAATTGAAGCTGCTGATATAGTTTTGATGAAAAGCAATTTGGAAGATGTAGTTACAGCCATAGATCTCTCTAGAAAGACTATGTCTCGAATTTGGTTGAATTATGTCTGGGCCCTGGGATACAACATCCTTGGCATGCCAGTTGCTGCGGGAATCTTGTTCCCTTTCACTGGGATCCGATTACCACCTTGGCTTGCCTGTGCCTGTATGGCTGCTTCATCTGTTAGTGTGGTATGTTCATCTCTCTTGTTGCAGTCTTATAAGAAGCCTTTGCATTTTCAGAACATTAAGTCTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

962

Amino Acids

103.93

Weight (kDa)

5.6

Isoelectric Point (pI)

35.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	40 - 99	3.4e-12	Heavy-metal-associated domain
HMA	PF00403	114 - 173	1.5e-09	Heavy-metal-associated domain
HMA	PF00403	203 - 247	4.7e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	421 - 525	7.7e-25	P-type ATPase actuator domain
Hydrolase	PF00702	634 - 852	2.6e-27	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2850
Acc36I	ACCTGC	1 cut(s)	281
AccB1I	GGYRCC	3 cut(s)	1073, 1487, 2579
AccB7I	CCANNNNNTGG	2 cut(s)	1716, 2371
AciI	CCGC	4 cut(s)	24, 90, 2200, 2738
AclWI	GGATC	7 cut(s)	458, 529, 1024, 2318, 2444, 2758, 2771
AcoI	YGGCCR	1 cut(s)	220
AcsI	RAATTY	5 cut(s)	308, 1694, 1994, 2237, 2679
AcuI	CTGAAG	1 cut(s)	612
AdeI	CACNNNGTG	1 cut(s)	1856
AfaI	GTAC	6 cut(s)	824, 923, 1199, 1773, 2002, 2130
AfiI	CCNNNNNNNGG	6 cut(s)	187, 218, 1388, 1716, 2371, 2759
AflII	CTTAAG	2 cut(s)	113, 1355
AflIII	ACRYGT	3 cut(s)	657, 2101, 2270
AgeI	ACCGGT	1 cut(s)	482
AhlI	ACTAGT	2 cut(s)	1160, 1774
AjiI	CACGTC	1 cut(s)	658
AjnI	CCWGG	4 cut(s)	1188, 1686, 2214, 2703
AjuI	GAANNNNNNNTTGG	2 cut(s)	2612, 2644
AleI	CACNNNNGTG	1 cut(s)	980
Alw21I	GWGCWC	5 cut(s)	252, 365, 531, 977, 1597
Alw26I	GTCTC	4 cut(s)	806, 1535, 2438, 2679
Alw44I	GTGCAC	2 cut(s)	361, 973
AlwI	GGATC	7 cut(s)	458, 529, 1024, 2318, 2444, 2758, 2771
AlwNI	CAGNNNCTG	3 cut(s)	1544, 2342, 2735
Ama87I	CYCGRG	1 cut(s)	1367
AoxI	GGCC	4 cut(s)	59, 220, 1448, 2700
ApaI	GGGCCC	1 cut(s)	2704
ApaLI	GTGCAC	2 cut(s)	361, 973
ApoI	RAATTY	5 cut(s)	308, 1694, 1994, 2237, 2679
AseI	ATTAAT	1 cut(s)	255
AsiGI	ACCGGT	1 cut(s)	482
AspLEI	GCGC	2 cut(s)	1549, 2576
AspS9I	GGNCC	4 cut(s)	704, 2222, 2700, 2701
AsuC2I	CCSGG	2 cut(s)	1368, 1369
AsuHPI	GGTGA	8 cut(s)	201, 667, 673, 1481, 1912, 2174, 2411, 2526
AsuII	TTCGAA	1 cut(s)	397
AsuNHI	GCTAGC	1 cut(s)	1609
AvaI	CYCGRG	1 cut(s)	1367
AvaII	GGWCC	2 cut(s)	704, 2222
AxyI	CCTNAGG	1 cut(s)	2226
BaeGI	GKGCMC	4 cut(s)	365, 977, 1076, 2704
BalI	TGGCCA	1 cut(s)	222
BamHI	GGATCC	1 cut(s)	2763
BanI	GGYRCC	3 cut(s)	1073, 1487, 2579
BanII	GRGCYC	4 cut(s)	75, 252, 531, 2704
BarI	GAAGNNNNNNTAC	2 cut(s)	2788, 2820
BbsI	GAAGAC	1 cut(s)	600
Bbv12I	GWGCWC	5 cut(s)	252, 365, 531, 977, 1597
BceAI	ACGGC	1 cut(s)	1284
BcgI	CGANNNNNNTGC	2 cut(s)	2407, 2441
BciT130I	CCWGG	4 cut(s)	1190, 1688, 2216, 2705
BciVI	GTATCC	1 cut(s)	2702
BclI	TGATCA	2 cut(s)	1431, 1615
BcnI	CCSGG	2 cut(s)	1368, 1369
BcoDI	GTCTC	4 cut(s)	806, 1535, 2438, 2679
BcuI	ACTAGT	2 cut(s)	1160, 1774
BfaI	CTAG	8 cut(s)	434, 1161, 1211, 1215, 1286, 1610, 1775, 2661
BfmI	CTRYAG	5 cut(s)	945, 1754, 1815, 2016, 2547
BfoI	RGCGCY	1 cut(s)	2577
BfrI	CTTAAG	2 cut(s)	113, 1355
BfuAI	ACCTGC	1 cut(s)	281
BfuI	GTATCC	1 cut(s)	2702
BglI	GCCNNNNNGGC	1 cut(s)	2798
BglII	AGATCT	3 cut(s)	710, 1620, 2653
BmcAI	AGTACT	2 cut(s)	1199, 1773
Bme1390I	CCNGG	6 cut(s)	1190, 1368, 1369, 1688, 2216, 2705
Bme18I	GGWCC	2 cut(s)	704, 2222
BmeT110I	CYCGRG	1 cut(s)	1367
BmgBI	CACGTC	1 cut(s)	658
BmgT120I	GGNCC	4 cut(s)	704, 2222, 2700, 2701
BmiI	GGNNCC	7 cut(s)	72, 830, 1075, 1489, 2581, 2702, 2765
BmrFI	CCNGG	6 cut(s)	1190, 1368, 1369, 1688, 2216, 2705
BmrI	ACTGGG	3 cut(s)	293, 1079, 2769
BmtI	GCTAGC	1 cut(s)	1613
BmuI	ACTGGG	3 cut(s)	293, 1079, 2769
BoxI	GACNNNNGTC	1 cut(s)	1039
BpiI	GAAGAC	1 cut(s)	600
BpmI	CTGGAG	1 cut(s)	2157
Bpu10I	CCTNAGC	3 cut(s)	341, 538, 2115
Bpu14I	TTCGAA	1 cut(s)	397
BpuEI	CTTGAG	3 cut(s)	461, 830, 862
BpuMI	CCSGG	2 cut(s)	1368, 1369
Bsa29I	ATCGAT	3 cut(s)	234, 2421, 2427
BsaI	GGTCTC	1 cut(s)	2438
BsaWI	WCCGGW	2 cut(s)	482, 862
BsaXI	ACNNNNNCTCC	2 cut(s)	2130, 2160
Bsc4I	CCNNNNNNNGG	6 cut(s)	187, 218, 1388, 1716, 2371, 2759
Bse118I	RCCGGY	3 cut(s)	57, 336, 482
Bse21I	CCTNAGG	1 cut(s)	2226
Bse3DI	GCAATG	3 cut(s)	324, 1116, 1306
BseBI	CCWGG	4 cut(s)	1190, 1688, 2216, 2705
BseCI	ATCGAT	3 cut(s)	234, 2421, 2427
BseGI	GGATG	9 cut(s)	37, 48, 52, 85, 606, 1060, 1511, 1683, 2715
BseLI	CCNNNNNNNGG	6 cut(s)	187, 218, 1388, 1716, 2371, 2759
BseMI	GCAATG	3 cut(s)	324, 1116, 1306
BseMII	CTCAG	8 cut(s)	355, 516, 552, 830, 1323, 1782, 2217, 2433
BseSI	GKGCMC	4 cut(s)	365, 977, 1076, 2704
BseYI	CCCAGC	2 cut(s)	1672, 2303
BsgI	GTGCAG	2 cut(s)	1004, 1563
BshFI	GGCC	4 cut(s)	61, 222, 1450, 2702
BshNI	GGYRCC	3 cut(s)	1073, 1487, 2579
BshTI	ACCGGT	1 cut(s)	482
BshVI	ATCGAT	3 cut(s)	234, 2421, 2427
BsiHKAI	GWGCWC	5 cut(s)	252, 365, 531, 977, 1597
BsiHKCI	CYCGRG	1 cut(s)	1367
BsiSI	CCGG	5 cut(s)	58, 337, 483, 863, 1368
BslFI	GGGAC	2 cut(s)	923, 1942
BslI	CCNNNNNNNGG	6 cut(s)	187, 218, 1388, 1716, 2371, 2759
BsmAI	GTCTC	4 cut(s)	806, 1535, 2438, 2679
BsmFI	GGGAC	2 cut(s)	923, 1942
BsmI	GAATGC	1 cut(s)	957
BsnI	GGCC	4 cut(s)	61, 222, 1450, 2702
Bso31I	GGTCTC	1 cut(s)	2438
BsoBI	CYCGRG	1 cut(s)	1367
Bsp119I	TTCGAA	1 cut(s)	397
Bsp120I	GGGCCC	1 cut(s)	2700
Bsp1286I	GDGCHC	8 cut(s)	75, 252, 365, 531, 977, 1076, 1597, 2704
Bsp1407I	TGTACA	2 cut(s)	822, 921
Bsp19I	CCATGG	1 cut(s)	2454
BspACI	CCGC	4 cut(s)	24, 90, 2200, 2738
BspANI	GGCC	4 cut(s)	61, 222, 1450, 2702
BspCNI	CTCAG	8 cut(s)	354, 517, 551, 829, 1322, 1781, 2218, 2434
BspDI	ATCGAT	3 cut(s)	234, 2421, 2427
BspHI	TCATGA	1 cut(s)	492
BspLI	GGNNCC	7 cut(s)	72, 830, 1075, 1489, 2581, 2702, 2765
BspMAI	CTGCAG	3 cut(s)	1758, 2020, 2551
BspMI	ACCTGC	1 cut(s)	281
BspOI	GCTAGC	1 cut(s)	1613
BspPI	GGATC	7 cut(s)	458, 529, 1024, 2318, 2444, 2758, 2771
BspQI	GCTCTTC	1 cut(s)	399
BspT104I	TTCGAA	1 cut(s)	397
BspT107I	GGYRCC	3 cut(s)	1073, 1487, 2579
BspTI	CTTAAG	2 cut(s)	113, 1355
BspTNI	GGTCTC	1 cut(s)	2438
BsrDI	GCAATG	3 cut(s)	324, 1116, 1306
BsrFI	RCCGGY	3 cut(s)	57, 336, 482
BsrGI	TGTACA	2 cut(s)	822, 921
BssAI	RCCGGY	3 cut(s)	57, 336, 482
BssT1I	CCWWGG	5 cut(s)	75, 782, 2454, 2719, 2777
Bst2UI	CCWGG	4 cut(s)	1190, 1688, 2216, 2705
Bst4CI	ACNGT	8 cut(s)	140, 205, 946, 1909, 1939, 1954, 2005, 2503
Bst6I	CTCTTC	3 cut(s)	399, 784, 2149
BstAFI	CTTAAG	2 cut(s)	113, 1355
BstAPI	GCANNNNNTGC	1 cut(s)	369
BstAUI	TGTACA	2 cut(s)	822, 921
BstBI	TTCGAA	1 cut(s)	397
BstDSI	CCRYGG	1 cut(s)	2454
BstEII	GGTNACC	1 cut(s)	673
BstF5I	GGATG	9 cut(s)	37, 48, 52, 85, 606, 1060, 1511, 1683, 2715
BstH2I	RGCGCY	1 cut(s)	2577
BstHHI	GCGC	2 cut(s)	1549, 2576
BstMAI	GTCTC	4 cut(s)	806, 1535, 2438, 2679
BstNI	CCWGG	4 cut(s)	1190, 1688, 2216, 2705
BstNSI	RCATGY	7 cut(s)	940, 1788, 2066, 2105, 2274, 2349, 2728
BstPAI	GACNNNNGTC	1 cut(s)	1039
BstPI	GGTNACC	1 cut(s)	673
BstSCI	CCNGG	6 cut(s)	1188, 1366, 1367, 1686, 2214, 2703
BstSFI	CTRYAG	5 cut(s)	945, 1754, 1815, 2016, 2547
BstSLI	GKGCMC	4 cut(s)	365, 977, 1076, 2704
BstV2I	GAAGAC	1 cut(s)	600
BstX2I	RGATCY	5 cut(s)	521, 710, 1620, 2653, 2763
BstXI	CCANNNNNNTGG	1 cut(s)	20
BstYI	RGATCY	5 cut(s)	521, 710, 1620, 2653, 2763
Bsu15I	ATCGAT	3 cut(s)	234, 2421, 2427
Bsu36I	CCTNAGG	1 cut(s)	2226
BsuI	GTATCC	1 cut(s)	2702
BsuRI	GGCC	4 cut(s)	61, 222, 1450, 2702
BsuTUI	ATCGAT	3 cut(s)	234, 2421, 2427
BtgI	CCRYGG	1 cut(s)	2454
BtrI	CACGTC	1 cut(s)	658
BtsCI	GGATG	9 cut(s)	37, 48, 52, 85, 606, 1060, 1511, 1683, 2715
BtsI	GCAGTG	1 cut(s)	1751
BveI	ACCTGC	1 cut(s)	281
CaiI	CAGNNNCTG	3 cut(s)	1544, 2342, 2735
CciI	TCATGA	1 cut(s)	492
CfoI	GCGC	2 cut(s)	1549, 2576
Cfr10I	RCCGGY	3 cut(s)	57, 336, 482
Cfr13I	GGNCC	4 cut(s)	704, 2222, 2700, 2701
Cfr9I	CCCGGG	1 cut(s)	1367
ClaI	ATCGAT	3 cut(s)	234, 2421, 2427
Csp6I	GTAC	6 cut(s)	823, 922, 1198, 1772, 2001, 2129
CspAI	ACCGGT	1 cut(s)	482
CviQI	GTAC	6 cut(s)	823, 922, 1198, 1772, 2001, 2129
DraIII	CACNNNGTG	1 cut(s)	1856
EaeI	YGGCCR	1 cut(s)	220
Eam1104I	CTCTTC	3 cut(s)	399, 784, 2149
EarI	CTCTTC	3 cut(s)	399, 784, 2149
EciI	GGCGGA	1 cut(s)	39
Ecl136II	GAGCTC	2 cut(s)	250, 529
Eco130I	CCWWGG	5 cut(s)	75, 782, 2454, 2719, 2777
Eco147I	AGGCCT	1 cut(s)	1450
Eco24I	GRGCYC	4 cut(s)	75, 252, 531, 2704
Eco31I	GGTCTC	1 cut(s)	2438
Eco32I	GATATC	3 cut(s)	52, 1176, 2425
Eco47I	GGWCC	2 cut(s)	704, 2222
Eco53kI	GAGCTC	2 cut(s)	250, 529
Eco57I	CTGAAG	1 cut(s)	612
Eco81I	CCTNAGG	1 cut(s)	2226
Eco88I	CYCGRG	1 cut(s)	1367
Eco91I	GGTNACC	1 cut(s)	673
EcoICRI	GAGCTC	2 cut(s)	250, 529
EcoO109I	RGGNCCY	1 cut(s)	2701
EcoO65I	GGTNACC	1 cut(s)	673
EcoRI	GAATTC	2 cut(s)	1694, 1994
EcoRII	CCWGG	4 cut(s)	1188, 1686, 2214, 2703
EcoRV	GATATC	3 cut(s)	52, 1176, 2425
EcoT14I	CCWWGG	5 cut(s)	75, 782, 2454, 2719, 2777
EcoT38I	GRGCYC	4 cut(s)	75, 252, 531, 2704
ErhI	CCWWGG	5 cut(s)	75, 782, 2454, 2719, 2777
FalI	AAGNNNNNCTT	4 cut(s)	443, 475, 1845, 1877
FaqI	GGGAC	2 cut(s)	923, 1942
FauI	CCCGC	2 cut(s)	2193, 2731
FbaI	TGATCA	2 cut(s)	1431, 1615
FokI	GGATG	9 cut(s)	44, 55, 59, 92, 593, 1067, 1518, 1670, 2702
FriOI	GRGCYC	4 cut(s)	75, 252, 531, 2704
FspBI	CTAG	8 cut(s)	434, 1161, 1211, 1215, 1286, 1610, 1775, 2661
GlaI	GCGC	2 cut(s)	1548, 2575
GsaI	CCCAGC	2 cut(s)	1676, 2307
GsuI	CTGGAG	1 cut(s)	2157
HaeII	RGCGCY	1 cut(s)	2577
HaeIII	GGCC	4 cut(s)	61, 222, 1450, 2702
HapII	CCGG	5 cut(s)	58, 337, 483, 863, 1368
HhaI	GCGC	2 cut(s)	1549, 2576
Hin6I	GCGC	2 cut(s)	1547, 2574
HinP1I	GCGC	2 cut(s)	1547, 2574
HincII	GTYRAC	1 cut(s)	2094
HindII	GTYRAC	1 cut(s)	2094
HindIII	AAGCTT	1 cut(s)	766
HpaII	CCGG	5 cut(s)	58, 337, 483, 863, 1368
HphI	GGTGA	8 cut(s)	201, 667, 673, 1481, 1912, 2174, 2411, 2526
Hpy166II	GTNNAC	9 cut(s)	363, 457, 553, 655, 975, 1420, 2033, 2094, 2131
Hpy8I	GTNNAC	9 cut(s)	363, 457, 553, 655, 975, 1420, 2033, 2094, 2131
HpyAV	CCTTC	2 cut(s)	569, 1834
HpyCH4III	ACNGT	8 cut(s)	140, 205, 946, 1909, 1939, 1954, 2005, 2503
HpyCH4IV	ACGT	2 cut(s)	657, 1036
HpySE526I	ACGT	2 cut(s)	657, 1036
HspAI	GCGC	2 cut(s)	1547, 2574
KroI	GCCGGC	1 cut(s)	336
KroNI	GCCGGC	1 cut(s)	338
Ksp22I	TGATCA	2 cut(s)	1431, 1615
LguI	GCTCTTC	1 cut(s)	399
LmnI	GCTCC	4 cut(s)	70, 377, 2059, 2138
MaeI	CTAG	8 cut(s)	434, 1161, 1211, 1215, 1286, 1610, 1775, 2661
MaeII	ACGT	2 cut(s)	657, 1036
MboII	GAAGA	9 cut(s)	296, 386, 518, 605, 743, 801, 1157, 2166, 2645
MfeI	CAATTG	2 cut(s)	2568, 2592
MflI	RGATCY	5 cut(s)	521, 710, 1620, 2653, 2763
MhlI	GDGCHC	8 cut(s)	75, 252, 365, 531, 977, 1076, 1597, 2704
MlsI	TGGCCA	1 cut(s)	222
MluNI	TGGCCA	1 cut(s)	222
MlyI	GAGTC	3 cut(s)	386, 808, 2525
MmeI	TCCRAC	2 cut(s)	48, 1921
Mox20I	TGGCCA	1 cut(s)	222
MroNI	GCCGGC	1 cut(s)	336
MscI	TGGCCA	1 cut(s)	222
MslI	CAYNNNNRTG	2 cut(s)	18, 980
Msp20I	TGGCCA	1 cut(s)	222
MspCI	CTTAAG	2 cut(s)	113, 1355
MspI	CCGG	5 cut(s)	58, 337, 483, 863, 1368
MspR9I	CCNGG	6 cut(s)	1190, 1368, 1369, 1688, 2216, 2705
MunI	CAATTG	2 cut(s)	2568, 2592
Mva1269I	GAATGC	1 cut(s)	957
MvaI	CCWGG	4 cut(s)	1190, 1688, 2216, 2705
NaeI	GCCGGC	1 cut(s)	338
NciI	CCSGG	2 cut(s)	1368, 1369
NcoI	CCATGG	1 cut(s)	2454
NgoMIV	GCCGGC	1 cut(s)	336
NheI	GCTAGC	1 cut(s)	1609
NlaIV	GGNNCC	7 cut(s)	72, 830, 1075, 1489, 2581, 2702, 2765
NmuCI	GTSAC	5 cut(s)	207, 673, 1469, 2162, 2503
NspI	RCATGY	7 cut(s)	940, 1788, 2066, 2105, 2274, 2349, 2728
NspV	TTCGAA	1 cut(s)	397
OliI	CACNNNNGTG	1 cut(s)	980
PaeI	GCATGC	3 cut(s)	1788, 2066, 2728
PagI	TCATGA	1 cut(s)	492
PasI	CCCWGGG	1 cut(s)	2704
PceI	AGGCCT	1 cut(s)	1450
PciI	ACATGT	2 cut(s)	2101, 2270
PciSI	GCTCTTC	1 cut(s)	399
PctI	GAATGC	1 cut(s)	957
PdiI	GCCGGC	1 cut(s)	338
PfeI	GAWTC	9 cut(s)	5, 164, 386, 623, 928, 967, 2472, 2523, 2742
PflFI	GACNNNGTC	1 cut(s)	2671
PflMI	CCANNNNNTGG	2 cut(s)	1716, 2371
PinAI	ACCGGT	1 cut(s)	482
PleI	GAGTC	3 cut(s)	385, 808, 2525
PpsI	GAGTC	3 cut(s)	385, 808, 2525
PscI	ACATGT	2 cut(s)	2101, 2270
PshAI	GACNNNNGTC	1 cut(s)	1039
PshBI	ATTAAT	1 cut(s)	255
PsiI	TTATAA	1 cut(s)	2850
Psp124BI	GAGCTC	2 cut(s)	252, 531
Psp6I	CCWGG	4 cut(s)	1188, 1686, 2214, 2703
PspEI	GGTNACC	1 cut(s)	673
PspFI	CCCAGC	2 cut(s)	1672, 2303
PspGI	CCWGG	4 cut(s)	1188, 1686, 2214, 2703
PspN4I	GGNNCC	7 cut(s)	72, 830, 1075, 1489, 2581, 2702, 2765
PspOMI	GGGCCC	1 cut(s)	2700
PspPI	GGNCC	4 cut(s)	704, 2222, 2700, 2701
PstI	CTGCAG	3 cut(s)	1758, 2020, 2551
PstNI	CAGNNNCTG	3 cut(s)	1544, 2342, 2735
PsuI	RGATCY	5 cut(s)	521, 710, 1620, 2653, 2763
PsyI	GACNNNGTC	1 cut(s)	2671
RsaI	GTAC	6 cut(s)	824, 923, 1199, 1773, 2002, 2130
RsaNI	GTAC	6 cut(s)	823, 922, 1198, 1772, 2001, 2129
RseI	CAYNNNNRTG	2 cut(s)	18, 980
SacI	GAGCTC	2 cut(s)	252, 531
SapI	GCTCTTC	1 cut(s)	399
Sau96I	GGNCC	4 cut(s)	704, 2222, 2700, 2701
ScaI	AGTACT	2 cut(s)	1199, 1773
SchI	GAGTC	3 cut(s)	386, 808, 2525
ScrFI	CCNGG	6 cut(s)	1190, 1368, 1369, 1688, 2216, 2705
SduI	GDGCHC	8 cut(s)	75, 252, 365, 531, 977, 1076, 1597, 2704
SfcI	CTRYAG	5 cut(s)	945, 1754, 1815, 2016, 2547
SfuI	TTCGAA	1 cut(s)	397
SinI	GGWCC	2 cut(s)	704, 2222
SmaI	CCCGGG	1 cut(s)	1369
SmiMI	CAYNNNNRTG	2 cut(s)	18, 980
SmlI	CTYRAG	5 cut(s)	113, 440, 809, 841, 1355
SmoI	CTYRAG	5 cut(s)	113, 440, 809, 841, 1355
SpeI	ACTAGT	2 cut(s)	1160, 1774
SphI	GCATGC	3 cut(s)	1788, 2066, 2728
SseBI	AGGCCT	1 cut(s)	1450
SsiI	CCGC	4 cut(s)	24, 90, 2200, 2738
SspMI	CTAG	8 cut(s)	434, 1161, 1211, 1215, 1286, 1610, 1775, 2661
SstI	GAGCTC	2 cut(s)	252, 531
StuI	AGGCCT	1 cut(s)	1450
StyD4I	CCNGG	6 cut(s)	1188, 1366, 1367, 1686, 2214, 2703
StyI	CCWWGG	5 cut(s)	75, 782, 2454, 2719, 2777
TaaI	ACNGT	8 cut(s)	140, 205, 946, 1909, 1939, 1954, 2005, 2503
TaiI	ACGT	2 cut(s)	660, 1039
TaqI	TCGA	7 cut(s)	234, 397, 488, 2266, 2421, 2427, 2677
TaqII	GACCGA	2 cut(s)	1688, 2462
TatI	WGTACW	5 cut(s)	822, 921, 1197, 1771, 2000
TauI	GCSGC	1 cut(s)	92
TfiI	GAWTC	9 cut(s)	5, 164, 386, 623, 928, 967, 2472, 2523, 2742
TseFI	GTSAC	5 cut(s)	207, 673, 1469, 2162, 2503
Tsp45I	GTSAC	5 cut(s)	207, 673, 1469, 2162, 2503
TspGWI	ACGGA	1 cut(s)	370
TspMI	CCCGGG	1 cut(s)	1367
Tth111I	GACNNNGTC	1 cut(s)	2671
Van91I	CCANNNNNTGG	2 cut(s)	1716, 2371
Vha464I	CTTAAG	2 cut(s)	113, 1355
VneI	GTGCAC	2 cut(s)	361, 973
VpaK11BI	GGWCC	2 cut(s)	704, 2222
VspI	ATTAAT	1 cut(s)	255
XapI	RAATTY	5 cut(s)	308, 1694, 1994, 2237, 2679
XbaI	TCTAGA	1 cut(s)	2660
XceI	RCATGY	7 cut(s)	940, 1788, 2066, 2105, 2274, 2349, 2728
XcmI	CCANNNNNNNNNTGG	2 cut(s)	166, 1984
XmaI	CCCGGG	1 cut(s)	1367
XspI	CTAG	8 cut(s)	434, 1161, 1211, 1215, 1286, 1610, 1775, 2661
ZrmI	AGTACT	2 cut(s)	1199, 1773

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.