Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

56022419 .. 56028092

Gene Structure 5674 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ33090

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2535 bp

ATGATGTGCACCAGTTGCTCCGAGTCCGTTGAATCTGCTCTTCGAATGGTTGATGGAGTGAAAAACGCAGTTGTTGGGCTAGCCCTTGAGGAAGCAAAGGTTCACTTTGATCCAAACATCACTGACACCTGTTCGATCATGAAAGCAATAGAAGATGCTGGCTTTGGATCTGAGCTCATTAGCTCAGGGAATGATGTAAACAAAGTGCATCTAAAACTTGAAGGAGTTAATTCTCCTGAAGACATGACCATCATCCAATCCTCCCTTGAATCAGTTGAGGGTGTAAATAATGTTGAAGTGGACGTGTTAGAAAAAAAGGTCACCATCACCTACGATGCAGACCTCACTGGTCCAAGATCTCTTATACATTGTATTGAAGAAGCTGGAAGCAACCCCAAATCATACCAAGCAAGCTTGCATGTTCCTCCAAGGCGAAGAGAAGCTGAACAGCAGCTTGAGACTCAGATGTACAGGAACCAGTTTTTCTTGAGCTGTCTATTTTCAGTTCCTGTATTTTTATTCTCAATGGTGCTTCCAATGCTTTCTCCCTATGGGGACTGGTTAATGTACAAGATTCACAACATGCTCACTGTAGGAATGCTTTTAAGATGGATTCTGTGCACACCCGTGCAGTTCATTATTGGCAGGAGGTTTTATGTGGGATCATATCATGCTTTAAGACGTAGGTCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTCTACTCTGTCTATATAGCAATGAAAGCATTGGCTTTGGATAATTTTGAGGGGGAAGATTTCTTTGAAACTAGTTCCATGATGATATCCTTTATACTCCTGGGGAAGTACTTAGAAGTTCTAGCTAGAGGAAAAACATCTGATGCTTTAGCAAAGCTGACAGACCTTACTCCTGAAACGGCTTATCTGCTATCACTAGATGCTGATGGCAATGCTATCTCAGAGATGGAAATTAGCACTCAACTTATTCAAAGAAATGATATACTTAAGATTGTTCCCGGGGCAAAAGTTCCTGTTGATGGGATTGTAATTAGTGGTCAAAGCCATGTGAACGAGAGTATGATCACAGGAGAGGCAAGGCCTATTTCTAAAAGATTAGGTGACAAGGTAATTGGTGGCACCATGAATGAGAATGGATGCTTACAGGTTAAGGCAACCCATGTTGGTTCAGAGACTGCGCTATCCCAGATAGTTCAACTTGTTGAAGCTGCACAGCTTTCCAGAGCACCTGTTCAGAAGCTAGCTGATCAGATCTCAAAGTTTTTCGTTCCAGCAGTTGTGATTGCAGCATTTCTGACATGGCTGGGTTGGTTCATCCTGGGAGAATTCGGTCTTTATCCCATATCATGGACACCAAAAGGCATGGATAGGTTTGAGCTGGCACTGCAGTTCGGCATCTCAGTACTAGTGGTTGCATGCCCTTGTGCTTTGGGATTAGCAACACCTACAGCAGTCATGGTTGCAACAGGGAAGGGTGCTACACAAGGTGTTCTTATCAAGGGTGGAAATGCACTTGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAGACAGGGACACTGACAGTTGGAAAACCGACAGTAGTTAGTGCTGTTCTATTTTCCAATTACTCAATGGAGGAATTCTGTACTGTGGCTACTGCTGCAGAGGCAAATAGTGAACACCCAATAGCAAAGTCTATTGTGGAGCATGCAAAGGGGTTTCTTAACAAGTTTGGGTCAACTGAACATGTGGTGGAGGCTAAGGACTTTGAGGTACACACTGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGGCTCATGCGGGAATATAATGTCCAGGTTGGTCCTGAGGTTGAGAAATTCGTTTCAGAAAATGAGAAACTGGCTCGAACATGTGTGTTAGTTTCCATTGATGGAAAGGTTGCTGGGTCGTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCATGTGTAATATCTTATCTCCACTCAATGGGCATTTCAAGCATCATGGTTACTGGTGATAATCAAAAAGTTGGCATCGATATCGATAAGGTGTTTGCTGAGACCGATCCCATGGGAAAAGCTGATAGAATCAAAAAGTTACAGATGAAAGGATTGACTGTGACTATGGTGGGAGATGGAATCAATGACTCACCAGCTTTGGCTGCAGCAGATGTTGGCATGGCAATTGGCGCTGGCACCAATGTAGCAATTGAAGCTGCTGATATAGTTTTGATGAAAAGCAATTTGGAAGATGTAGTTACAGCCATAGATCTCTCTAGAAAGACTATGTCTCGAATTTGGTTGAATTATGTCTGGGCCCTGGGATACAACATCCTTGGCATGCCAGTTGCTGCGGGAATCTTGTTCCCTTTCACTGGGATCCGATTACCACCTTGGCTTGCCTGTGCCTGTATGGCTGCTTCATCTGTTAGTGTGGTATGTTCATCTCTCTTGTTGCAGTCTTATAAGAAGCCTTTGCATTTTCAGAACATTAAGTCTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

844

Amino Acids

91.24

Weight (kDa)

5.78

Isoelectric Point (pI)

36.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	1 - 55	2e-06	Heavy-metal-associated domain
HMA	PF00403	85 - 129	4.1e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	303 - 407	6.4e-25	P-type ATPase actuator domain
Hydrolase	PF00702	516 - 734	2.1e-27	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2496
AccB1I	GGYRCC	3 cut(s)	719, 1133, 2225
AccB7I	CCANNNNNTGG	2 cut(s)	1362, 2017
AciI	CCGC	2 cut(s)	1846, 2384
AclWI	GGATC	7 cut(s)	104, 175, 670, 1964, 2090, 2404, 2417
AcsI	RAATTY	4 cut(s)	1340, 1640, 1883, 2325
AcuI	CTGAAG	1 cut(s)	258
AdeI	CACNNNGTG	1 cut(s)	1502
AfaI	GTAC	6 cut(s)	470, 569, 845, 1419, 1648, 1776
AfiI	CCNNNNNNNGG	4 cut(s)	1034, 1362, 2017, 2405
AflII	CTTAAG	1 cut(s)	1001
AflIII	ACRYGT	3 cut(s)	303, 1747, 1916
AhlI	ACTAGT	2 cut(s)	806, 1420
AjiI	CACGTC	1 cut(s)	304
AjnI	CCWGG	4 cut(s)	834, 1332, 1860, 2349
AjuI	GAANNNNNNNTTGG	2 cut(s)	2258, 2290
AleI	CACNNNNGTG	1 cut(s)	626
Alw21I	GWGCWC	4 cut(s)	11, 177, 623, 1243
Alw26I	GTCTC	4 cut(s)	452, 1181, 2084, 2325
Alw44I	GTGCAC	2 cut(s)	7, 619
AlwI	GGATC	7 cut(s)	104, 175, 670, 1964, 2090, 2404, 2417
AlwNI	CAGNNNCTG	4 cut(s)	509, 1190, 1988, 2381
Ama87I	CYCGRG	1 cut(s)	1013
AoxI	GGCC	2 cut(s)	1094, 2346
ApaI	GGGCCC	1 cut(s)	2350
ApaLI	GTGCAC	2 cut(s)	7, 619
ApoI	RAATTY	4 cut(s)	1340, 1640, 1883, 2325
AspLEI	GCGC	2 cut(s)	1195, 2222
AspS9I	GGNCC	4 cut(s)	350, 1868, 2346, 2347
AsuC2I	CCSGG	2 cut(s)	1014, 1015
AsuHPI	GGTGA	7 cut(s)	313, 319, 1127, 1558, 1820, 2057, 2172
AsuII	TTCGAA	1 cut(s)	43
AsuNHI	GCTAGC	2 cut(s)	79, 1255
AvaI	CYCGRG	1 cut(s)	1013
AvaII	GGWCC	2 cut(s)	350, 1868
AxyI	CCTNAGG	1 cut(s)	1872
BaeGI	GKGCMC	4 cut(s)	11, 623, 722, 2350
BamHI	GGATCC	1 cut(s)	2409
BanI	GGYRCC	3 cut(s)	719, 1133, 2225
BanII	GRGCYC	2 cut(s)	177, 2350
BarI	GAAGNNNNNNTAC	2 cut(s)	2434, 2466
BbsI	GAAGAC	1 cut(s)	246
Bbv12I	GWGCWC	4 cut(s)	11, 177, 623, 1243
BccI	CCATC	9 cut(s)	47, 257, 332, 603, 935, 955, 1028, 1931, 2159
BceAI	ACGGC	1 cut(s)	930
BcgI	CGANNNNNNTGC	2 cut(s)	2053, 2087
BciT130I	CCWGG	4 cut(s)	836, 1334, 1862, 2351
BciVI	GTATCC	1 cut(s)	2348
BclI	TGATCA	2 cut(s)	1077, 1261
BcnI	CCSGG	2 cut(s)	1014, 1015
BcoDI	GTCTC	4 cut(s)	452, 1181, 2084, 2325
BcuI	ACTAGT	2 cut(s)	806, 1420
BfaI	CTAG	8 cut(s)	80, 807, 857, 861, 932, 1256, 1421, 2307
BfmI	CTRYAG	5 cut(s)	591, 1400, 1461, 1662, 2193
BfoI	RGCGCY	1 cut(s)	2223
BfrI	CTTAAG	1 cut(s)	1001
BfuI	GTATCC	1 cut(s)	2348
BglI	GCCNNNNNGGC	1 cut(s)	2444
BglII	AGATCT	3 cut(s)	356, 1266, 2299
BmcAI	AGTACT	2 cut(s)	845, 1419
Bme1390I	CCNGG	6 cut(s)	836, 1014, 1015, 1334, 1862, 2351
Bme18I	GGWCC	2 cut(s)	350, 1868
BmeT110I	CYCGRG	1 cut(s)	1013
BmgBI	CACGTC	1 cut(s)	304
BmgT120I	GGNCC	4 cut(s)	350, 1868, 2346, 2347
BmiI	GGNNCC	6 cut(s)	476, 721, 1135, 2227, 2348, 2411
BmrFI	CCNGG	6 cut(s)	836, 1014, 1015, 1334, 1862, 2351
BmrI	ACTGGG	2 cut(s)	725, 2415
BmsI	GCATC	9 cut(s)	145, 217, 325, 868, 925, 1142, 1419, 2040, 2073
BmtI	GCTAGC	2 cut(s)	83, 1259
BmuI	ACTGGG	2 cut(s)	725, 2415
BoxI	GACNNNNGTC	1 cut(s)	685
BpiI	GAAGAC	1 cut(s)	246
BpmI	CTGGAG	1 cut(s)	1803
Bpu10I	CCTNAGC	2 cut(s)	184, 1761
Bpu14I	TTCGAA	1 cut(s)	43
BpuEI	CTTGAG	3 cut(s)	107, 476, 508
BpuMI	CCSGG	2 cut(s)	1014, 1015
Bsa29I	ATCGAT	2 cut(s)	2067, 2073
BsaI	GGTCTC	1 cut(s)	2084
BsaXI	ACNNNNNCTCC	2 cut(s)	1776, 1806
Bsc4I	CCNNNNNNNGG	4 cut(s)	1034, 1362, 2017, 2405
Bse21I	CCTNAGG	1 cut(s)	1872
Bse3DI	GCAATG	2 cut(s)	762, 952
BseBI	CCWGG	4 cut(s)	836, 1334, 1862, 2351
BseCI	ATCGAT	2 cut(s)	2067, 2073
BseGI	GGATG	5 cut(s)	252, 706, 1157, 1329, 2361
BseLI	CCNNNNNNNGG	4 cut(s)	1034, 1362, 2017, 2405
BseMI	GCAATG	2 cut(s)	762, 952
BseMII	CTCAG	7 cut(s)	162, 198, 476, 969, 1428, 1863, 2079
BseSI	GKGCMC	4 cut(s)	11, 623, 722, 2350
BseYI	CCCAGC	2 cut(s)	1318, 1949
BsgI	GTGCAG	2 cut(s)	650, 1209
BshFI	GGCC	2 cut(s)	1096, 2348
BshNI	GGYRCC	3 cut(s)	719, 1133, 2225
BshVI	ATCGAT	2 cut(s)	2067, 2073
BsiHKAI	GWGCWC	4 cut(s)	11, 177, 623, 1243
BsiHKCI	CYCGRG	1 cut(s)	1013
BsiSI	CCGG	1 cut(s)	1014
BslFI	GGGAC	2 cut(s)	569, 1588
BslI	CCNNNNNNNGG	4 cut(s)	1034, 1362, 2017, 2405
BsmAI	GTCTC	4 cut(s)	452, 1181, 2084, 2325
BsmFI	GGGAC	2 cut(s)	569, 1588
BsmI	GAATGC	1 cut(s)	603
BsnI	GGCC	2 cut(s)	1096, 2348
Bso31I	GGTCTC	1 cut(s)	2084
BsoBI	CYCGRG	1 cut(s)	1013
Bsp119I	TTCGAA	1 cut(s)	43
Bsp120I	GGGCCC	1 cut(s)	2346
Bsp1286I	GDGCHC	6 cut(s)	11, 177, 623, 722, 1243, 2350
Bsp1407I	TGTACA	2 cut(s)	468, 567
Bsp19I	CCATGG	1 cut(s)	2100
BspACI	CCGC	2 cut(s)	1846, 2384
BspANI	GGCC	2 cut(s)	1096, 2348
BspCNI	CTCAG	7 cut(s)	163, 197, 475, 968, 1427, 1864, 2080
BspDI	ATCGAT	2 cut(s)	2067, 2073
BspHI	TCATGA	1 cut(s)	138
BspLI	GGNNCC	6 cut(s)	476, 721, 1135, 2227, 2348, 2411
BspMAI	CTGCAG	3 cut(s)	1404, 1666, 2197
BspOI	GCTAGC	2 cut(s)	83, 1259
BspPI	GGATC	7 cut(s)	104, 175, 670, 1964, 2090, 2404, 2417
BspQI	GCTCTTC	1 cut(s)	45
BspT104I	TTCGAA	1 cut(s)	43
BspT107I	GGYRCC	3 cut(s)	719, 1133, 2225
BspTI	CTTAAG	1 cut(s)	1001
BspTNI	GGTCTC	1 cut(s)	2084
BsrDI	GCAATG	2 cut(s)	762, 952
BsrGI	TGTACA	2 cut(s)	468, 567
BssT1I	CCWWGG	4 cut(s)	428, 2100, 2365, 2423
Bst2UI	CCWGG	4 cut(s)	836, 1334, 1862, 2351
Bst4CI	ACNGT	6 cut(s)	592, 1555, 1585, 1600, 1651, 2149
Bst6I	CTCTTC	3 cut(s)	45, 430, 1795
BstAFI	CTTAAG	1 cut(s)	1001
BstAPI	GCANNNNNTGC	1 cut(s)	15
BstAUI	TGTACA	2 cut(s)	468, 567
BstBI	TTCGAA	1 cut(s)	43
BstDEI	CTNAG	9 cut(s)	171, 184, 462, 847, 955, 1414, 1761, 1872, 2088
BstDSI	CCRYGG	1 cut(s)	2100
BstEII	GGTNACC	1 cut(s)	319
BstF5I	GGATG	5 cut(s)	252, 706, 1157, 1329, 2361
BstH2I	RGCGCY	1 cut(s)	2223
BstHHI	GCGC	2 cut(s)	1195, 2222
BstMAI	GTCTC	4 cut(s)	452, 1181, 2084, 2325
BstNI	CCWGG	4 cut(s)	836, 1334, 1862, 2351
BstNSI	RCATGY	8 cut(s)	422, 586, 1434, 1712, 1751, 1920, 1995, 2374
BstPAI	GACNNNNGTC	1 cut(s)	685
BstPI	GGTNACC	1 cut(s)	319
BstSCI	CCNGG	6 cut(s)	834, 1012, 1013, 1332, 1860, 2349
BstSFI	CTRYAG	5 cut(s)	591, 1400, 1461, 1662, 2193
BstSLI	GKGCMC	4 cut(s)	11, 623, 722, 2350
BstV2I	GAAGAC	1 cut(s)	246
BstX2I	RGATCY	5 cut(s)	167, 356, 1266, 2299, 2409
BstYI	RGATCY	5 cut(s)	167, 356, 1266, 2299, 2409
Bsu15I	ATCGAT	2 cut(s)	2067, 2073
Bsu36I	CCTNAGG	1 cut(s)	1872
BsuI	GTATCC	1 cut(s)	2348
BsuRI	GGCC	2 cut(s)	1096, 2348
BsuTUI	ATCGAT	2 cut(s)	2067, 2073
BtgI	CCRYGG	1 cut(s)	2100
BtrI	CACGTC	1 cut(s)	304
BtsCI	GGATG	5 cut(s)	252, 706, 1157, 1329, 2361
BtsI	GCAGTG	1 cut(s)	1397
BtsIMutI	CAGTG	9 cut(s)	120, 345, 588, 713, 1397, 1577, 1779, 1980, 2403
CaiI	CAGNNNCTG	4 cut(s)	509, 1190, 1988, 2381
CciI	TCATGA	1 cut(s)	138
CfoI	GCGC	2 cut(s)	1195, 2222
Cfr13I	GGNCC	4 cut(s)	350, 1868, 2346, 2347
Cfr9I	CCCGGG	1 cut(s)	1013
ClaI	ATCGAT	2 cut(s)	2067, 2073
Csp6I	GTAC	6 cut(s)	469, 568, 844, 1418, 1647, 1775
CviQI	GTAC	6 cut(s)	469, 568, 844, 1418, 1647, 1775
DdeI	CTNAG	9 cut(s)	171, 184, 462, 847, 955, 1414, 1761, 1872, 2088
DraIII	CACNNNGTG	1 cut(s)	1502
Eam1104I	CTCTTC	3 cut(s)	45, 430, 1795
EarI	CTCTTC	3 cut(s)	45, 430, 1795
Ecl136II	GAGCTC	1 cut(s)	175
Eco130I	CCWWGG	4 cut(s)	428, 2100, 2365, 2423
Eco147I	AGGCCT	1 cut(s)	1096
Eco24I	GRGCYC	2 cut(s)	177, 2350
Eco31I	GGTCTC	1 cut(s)	2084
Eco32I	GATATC	2 cut(s)	822, 2071
Eco47I	GGWCC	2 cut(s)	350, 1868
Eco53kI	GAGCTC	1 cut(s)	175
Eco57I	CTGAAG	1 cut(s)	258
Eco81I	CCTNAGG	1 cut(s)	1872
Eco88I	CYCGRG	1 cut(s)	1013
Eco91I	GGTNACC	1 cut(s)	319
EcoICRI	GAGCTC	1 cut(s)	175
EcoO109I	RGGNCCY	1 cut(s)	2347
EcoO65I	GGTNACC	1 cut(s)	319
EcoRI	GAATTC	2 cut(s)	1340, 1640
EcoRII	CCWGG	4 cut(s)	834, 1332, 1860, 2349
EcoRV	GATATC	2 cut(s)	822, 2071
EcoT14I	CCWWGG	4 cut(s)	428, 2100, 2365, 2423
EcoT38I	GRGCYC	2 cut(s)	177, 2350
ErhI	CCWWGG	4 cut(s)	428, 2100, 2365, 2423
FalI	AAGNNNNNCTT	4 cut(s)	89, 121, 1491, 1523
FaqI	GGGAC	2 cut(s)	569, 1588
FauI	CCCGC	2 cut(s)	1839, 2377
FbaI	TGATCA	2 cut(s)	1077, 1261
FokI	GGATG	5 cut(s)	239, 713, 1164, 1316, 2348
FriOI	GRGCYC	2 cut(s)	177, 2350
FspBI	CTAG	8 cut(s)	80, 807, 857, 861, 932, 1256, 1421, 2307
GlaI	GCGC	2 cut(s)	1194, 2221
GsaI	CCCAGC	2 cut(s)	1322, 1953
GsuI	CTGGAG	1 cut(s)	1803
HaeII	RGCGCY	1 cut(s)	2223
HaeIII	GGCC	2 cut(s)	1096, 2348
HapII	CCGG	1 cut(s)	1014
HhaI	GCGC	2 cut(s)	1195, 2222
Hin6I	GCGC	2 cut(s)	1193, 2220
HinP1I	GCGC	2 cut(s)	1193, 2220
HincII	GTYRAC	1 cut(s)	1740
HindII	GTYRAC	1 cut(s)	1740
HindIII	AAGCTT	1 cut(s)	412
HpaII	CCGG	1 cut(s)	1014
HphI	GGTGA	7 cut(s)	313, 319, 1127, 1558, 1820, 2057, 2172
Hpy166II	GTNNAC	9 cut(s)	9, 103, 199, 301, 621, 1066, 1679, 1740, 1777
Hpy188III	TCNNGA	8 cut(s)	139, 236, 487, 908, 1236, 1871, 2307, 2322
Hpy8I	GTNNAC	9 cut(s)	9, 103, 199, 301, 621, 1066, 1679, 1740, 1777
HpyAV	CCTTC	2 cut(s)	215, 1480
HpyCH4III	ACNGT	6 cut(s)	592, 1555, 1585, 1600, 1651, 2149
HpyCH4IV	ACGT	2 cut(s)	303, 682
HpyF3I	CTNAG	9 cut(s)	171, 184, 462, 847, 955, 1414, 1761, 1872, 2088
HpySE526I	ACGT	2 cut(s)	303, 682
HspAI	GCGC	2 cut(s)	1193, 2220
Ksp22I	TGATCA	2 cut(s)	1077, 1261
LguI	GCTCTTC	1 cut(s)	45
LmnI	GCTCC	3 cut(s)	23, 1705, 1784
LweI	GCATC	9 cut(s)	145, 217, 325, 868, 925, 1142, 1419, 2040, 2073
MaeI	CTAG	8 cut(s)	80, 807, 857, 861, 932, 1256, 1421, 2307
MaeII	ACGT	2 cut(s)	303, 682
MaeIII	GTNAC	8 cut(s)	319, 1115, 1808, 1963, 2038, 2127, 2149, 2287
MboII	GAAGA	8 cut(s)	32, 164, 251, 389, 447, 803, 1812, 2291
MfeI	CAATTG	2 cut(s)	2214, 2238
MflI	RGATCY	5 cut(s)	167, 356, 1266, 2299, 2409
MhlI	GDGCHC	6 cut(s)	11, 177, 623, 722, 1243, 2350
MlyI	GAGTC	3 cut(s)	32, 454, 2171
MmeI	TCCRAC	1 cut(s)	1567
MseI	TTAA	8 cut(s)	228, 563, 605, 677, 1002, 1164, 1725, 2523
MslI	CAYNNNNRTG	1 cut(s)	626
MspCI	CTTAAG	1 cut(s)	1001
MspI	CCGG	1 cut(s)	1014
MspR9I	CCNGG	6 cut(s)	836, 1014, 1015, 1334, 1862, 2351
MunI	CAATTG	2 cut(s)	2214, 2238
Mva1269I	GAATGC	1 cut(s)	603
MvaI	CCWGG	4 cut(s)	836, 1334, 1862, 2351
NciI	CCSGG	2 cut(s)	1014, 1015
NcoI	CCATGG	1 cut(s)	2100
NheI	GCTAGC	2 cut(s)	79, 1255
NlaIV	GGNNCC	6 cut(s)	476, 721, 1135, 2227, 2348, 2411
NmuCI	GTSAC	4 cut(s)	319, 1115, 1808, 2149
NspI	RCATGY	8 cut(s)	422, 586, 1434, 1712, 1751, 1920, 1995, 2374
NspV	TTCGAA	1 cut(s)	43
OliI	CACNNNNGTG	1 cut(s)	626
PaeI	GCATGC	3 cut(s)	1434, 1712, 2374
PagI	TCATGA	1 cut(s)	138
PasI	CCCWGGG	1 cut(s)	2350
PceI	AGGCCT	1 cut(s)	1096
PciI	ACATGT	2 cut(s)	1747, 1916
PciSI	GCTCTTC	1 cut(s)	45
PctI	GAATGC	1 cut(s)	603
PfeI	GAWTC	7 cut(s)	32, 269, 574, 613, 2118, 2169, 2388
PflFI	GACNNNGTC	1 cut(s)	2317
PflMI	CCANNNNNTGG	2 cut(s)	1362, 2017
PleI	GAGTC	3 cut(s)	31, 454, 2171
PpsI	GAGTC	3 cut(s)	31, 454, 2171
PscI	ACATGT	2 cut(s)	1747, 1916
PshAI	GACNNNNGTC	1 cut(s)	685
PsiI	TTATAA	1 cut(s)	2496
Psp124BI	GAGCTC	1 cut(s)	177
Psp6I	CCWGG	4 cut(s)	834, 1332, 1860, 2349
PspEI	GGTNACC	1 cut(s)	319
PspFI	CCCAGC	2 cut(s)	1318, 1949
PspGI	CCWGG	4 cut(s)	834, 1332, 1860, 2349
PspN4I	GGNNCC	6 cut(s)	476, 721, 1135, 2227, 2348, 2411
PspOMI	GGGCCC	1 cut(s)	2346
PspPI	GGNCC	4 cut(s)	350, 1868, 2346, 2347
PstI	CTGCAG	3 cut(s)	1404, 1666, 2197
PstNI	CAGNNNCTG	4 cut(s)	509, 1190, 1988, 2381
PsuI	RGATCY	5 cut(s)	167, 356, 1266, 2299, 2409
PsyI	GACNNNGTC	1 cut(s)	2317
RsaI	GTAC	6 cut(s)	470, 569, 845, 1419, 1648, 1776
RsaNI	GTAC	6 cut(s)	469, 568, 844, 1418, 1647, 1775
RseI	CAYNNNNRTG	1 cut(s)	626
SacI	GAGCTC	1 cut(s)	177
SapI	GCTCTTC	1 cut(s)	45
SaqAI	TTAA	8 cut(s)	228, 563, 605, 677, 1002, 1164, 1725, 2523
Sau96I	GGNCC	4 cut(s)	350, 1868, 2346, 2347
ScaI	AGTACT	2 cut(s)	845, 1419
SchI	GAGTC	3 cut(s)	32, 454, 2171
ScrFI	CCNGG	6 cut(s)	836, 1014, 1015, 1334, 1862, 2351
SduI	GDGCHC	6 cut(s)	11, 177, 623, 722, 1243, 2350
SfaNI	GCATC	9 cut(s)	145, 217, 325, 868, 925, 1142, 1419, 2040, 2073
SfcI	CTRYAG	5 cut(s)	591, 1400, 1461, 1662, 2193
SfuI	TTCGAA	1 cut(s)	43
SinI	GGWCC	2 cut(s)	350, 1868
SmaI	CCCGGG	1 cut(s)	1015
SmiMI	CAYNNNNRTG	1 cut(s)	626
SmlI	CTYRAG	4 cut(s)	86, 455, 487, 1001
SmoI	CTYRAG	4 cut(s)	86, 455, 487, 1001
SpeI	ACTAGT	2 cut(s)	806, 1420
SphI	GCATGC	3 cut(s)	1434, 1712, 2374
SseBI	AGGCCT	1 cut(s)	1096
SsiI	CCGC	2 cut(s)	1846, 2384
SspMI	CTAG	8 cut(s)	80, 807, 857, 861, 932, 1256, 1421, 2307
SstI	GAGCTC	1 cut(s)	177
StuI	AGGCCT	1 cut(s)	1096
StyD4I	CCNGG	6 cut(s)	834, 1012, 1013, 1332, 1860, 2349
StyI	CCWWGG	4 cut(s)	428, 2100, 2365, 2423
TaaI	ACNGT	6 cut(s)	592, 1555, 1585, 1600, 1651, 2149
TaiI	ACGT	2 cut(s)	306, 685
TaqI	TCGA	6 cut(s)	43, 134, 1912, 2067, 2073, 2323
TaqII	GACCGA	2 cut(s)	1334, 2108
TatI	WGTACW	5 cut(s)	468, 567, 843, 1417, 1646
TfiI	GAWTC	7 cut(s)	32, 269, 574, 613, 2118, 2169, 2388
Tru1I	TTAA	8 cut(s)	228, 563, 605, 677, 1002, 1164, 1725, 2523
Tru9I	TTAA	8 cut(s)	228, 563, 605, 677, 1002, 1164, 1725, 2523
TscAI	CASTG	9 cut(s)	127, 352, 595, 720, 1404, 1584, 1786, 1980, 2410
TseFI	GTSAC	4 cut(s)	319, 1115, 1808, 2149
Tsp45I	GTSAC	4 cut(s)	319, 1115, 1808, 2149
TspDTI	ATGAA	9 cut(s)	155, 625, 773, 1154, 1318, 2150, 2279, 2442, 2463
TspGWI	ACGGA	1 cut(s)	16
TspMI	CCCGGG	1 cut(s)	1013
TspRI	CASTG	9 cut(s)	127, 352, 595, 720, 1404, 1584, 1786, 1980, 2410
Tth111I	GACNNNGTC	1 cut(s)	2317
Van91I	CCANNNNNTGG	2 cut(s)	1362, 2017
Vha464I	CTTAAG	1 cut(s)	1001
VneI	GTGCAC	2 cut(s)	7, 619
VpaK11BI	GGWCC	2 cut(s)	350, 1868
XapI	RAATTY	4 cut(s)	1340, 1640, 1883, 2325
XbaI	TCTAGA	1 cut(s)	2306
XceI	RCATGY	8 cut(s)	422, 586, 1434, 1712, 1751, 1920, 1995, 2374
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1630
XmaI	CCCGGG	1 cut(s)	1013
XspI	CTAG	8 cut(s)	80, 807, 857, 861, 932, 1256, 1421, 2307
ZrmI	AGTACT	2 cut(s)	845, 1419

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.