Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

57621220 .. 57628790

Gene Structure 7571 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG378500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGATGTGCACCAGTTGCTCCGAGTCCGTTGAATCTGCTCTTCGAATGGTTGATGGAGTGAAAAACGCAGTTGTTGGGCTAGCCCTTGAGGAAGCAAAGGTTCACTTTGATCCAAACATCACTGACACCTGTTCGATCATGAAAGCAATAGAAGATGCTGGCTTTGGATCTGAGCTCATTAGCTCAGGGAATGATGTAAACAAAGTGCATCTAAAACTTGAAGGAGTTAATTCTCCTGAAGACATGACCATCATCCAATCCTCCCTTGAATCAGTTGAGGGTGTAAATAATGTTGAAGTGGACGTGTTAGAAACAAAGGTCACCATCACCTACGATGCAGACCTCACTGGTCCAAGATCTCTTATACATTGTATTGAAGAAGCTGGAAGCAACCCCAAATCATACCAAGCAAGCTTGCATGTTCCTCCAAGGCGAAGAGAAGCTGAACAGCAGCTTGAGACTCAGATGTACAGGAACCAGTTTTTCTTGAGCTGTCTATTTTCAGTTCCTGTATTTTTATTCTCAATGGTGCTTCCAATGCTTTCTCCCTATGGGGACTGGTTAATGTACAAGATTCACAACATGCTCACTGTAGGAATGCTTTTAAGATGGATTCTGTGCACACCCGTGCAGTTCATTATTGGCAGGAGGTTTTATGTGGGATCATATCATGCTTTAAGACGTAGGTCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTCTACTCTGTCTATATAGCAATGAAAGCATTGGCTTTGGATAATTTTGAGGGGGAAGATTTCTTTGAAACTAGTTCCATGATGATATCCTTTATACTCCTGGGGAAGTACTTAGAAGTTCTAGCTAGAGGAAAAACATCTGATGCTTTAGCAAAGCTGACAGACCTTACTCCTGAAACGGCTTATCTGCTATCACTAGATGCTGATGGCAATGCTATCTCAGAGATGGAAATTAGCACTCAACTTATTCAAAGAAATGATATACTTAAGATTGTTCCCGGGGCAAAAGTTCCTGTTGATGGGATTGTAATTAGTGGTCAAAGCCATGTGAACGAGAGTATGATCACAGGAGAGGCAAGGCCTATTTCTAAAAGATTAGGTGACAAGGTAATTGGTGGCACCATGAATGAGAATGGATGCTTACAGGTTAAGGCAACCCATGTTGGTTCAGAGACTGCGCTATCCCAGATAGTTCAACTTGTTGAAGCTGCACAGCTTTCCAGAGCACCTGTTCAGAAGCTAGCTGATCAGATCTCAAAGTTTTTTGTTCCAGCAGTTGTGATTGCAGCATTTCTGACATGGCTGGGTTGGTTCATCCTGGGAGAATTCGGTCTTTATCCCATATCATGGACACCAAAAGGCATGGATAGGTTTGAGCTGGCACTGCAGTTCGGCATCTCAGTACTAGTGGTTGCATGCCCTTGTGCTTTGGGATTAGCAACACCTACAGCAGTCATGGTTGCAACAGGGAAGGGTGCTACACAAGGTGTTTTTATCAAGGGTGGAAATGCACTTGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAGACAGGGACACTGACAGTTGGAAAACCGACAGTAGTTAGTGCTGTTCTATTTTCCAATTACTCAATGGAGGAATTCTGTACTGTGGCTACTGCTGCAGAGGCAAATAGTGAACACCCAATAGCAAAGTCTATTGTGGAGCATGCAAAGGGGTTTCTTAATAAGTTTGGATCAACTGAACATGTGGTGGAGGCTAAGGACTTTGAGGTACACACTGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGGCTCATGCGGGAATATAATGTCCAGGTTGGTCCTGAGGTTGAGAAATTCGTTTCAGAAAATGAGAAACTGGCTCGAACATGTGTGTTAGTTTCCATTGATGGAAAGGTTGCTGGGTCGTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCATGTGTAATATCTTATCTACACTCAATGGGCATTTCAAGCATCATGGTTACTGGTGATAATTGGGCAACAGCAGCAGCAATTGCAAGGGAGGTTGGCATCGATAAGGTGTTTGCTGAGACCGATCCCATGGGAAAAGCTGATAGAATCAAAGAGTTACAGGTAAGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

720

Amino Acids

78.11

Weight (kDa)

5.47

Isoelectric Point (pI)

32.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	1 - 55	1.7e-06	Heavy-metal-associated domain
HMA	PF00403	85 - 129	2.8e-06	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	303 - 407	5.1e-25	P-type ATPase actuator domain
Hydrolase	PF00702	516 - 718	1.2e-24	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	719, 1133
AccB7I	CCANNNNNTGG	1 cut(s)	1362
AciI	CCGC	1 cut(s)	1846
AclWI	GGATC	6 cut(s)	104, 175, 670, 1744, 1964, 2108
AcsI	RAATTY	3 cut(s)	1340, 1640, 1883
AcuI	CTGAAG	1 cut(s)	258
AdeI	CACNNNGTG	1 cut(s)	1502
AfaI	GTAC	6 cut(s)	470, 569, 845, 1419, 1648, 1776
AfiI	CCNNNNNNNGG	2 cut(s)	1034, 1362
AflII	CTTAAG	1 cut(s)	1001
AflIII	ACRYGT	3 cut(s)	303, 1747, 1916
AhlI	ACTAGT	2 cut(s)	806, 1420
AjiI	CACGTC	1 cut(s)	304
AjnI	CCWGG	3 cut(s)	834, 1332, 1860
AleI	CACNNNNGTG	1 cut(s)	626
Alw21I	GWGCWC	4 cut(s)	11, 177, 623, 1243
Alw26I	GTCTC	3 cut(s)	452, 1181, 2102
Alw44I	GTGCAC	2 cut(s)	7, 619
AlwI	GGATC	6 cut(s)	104, 175, 670, 1744, 1964, 2108
AlwNI	CAGNNNCTG	3 cut(s)	509, 1190, 1988
Ama87I	CYCGRG	1 cut(s)	1013
AoxI	GGCC	1 cut(s)	1094
ApaLI	GTGCAC	2 cut(s)	7, 619
ApeKI	GCWGC	7 cut(s)	451, 1223, 1301, 1661, 1988, 2063, 2066
ApoI	RAATTY	3 cut(s)	1340, 1640, 1883
AspLEI	GCGC	1 cut(s)	1195
AspS9I	GGNCC	2 cut(s)	350, 1868
AsuC2I	CCSGG	2 cut(s)	1014, 1015
AsuHPI	GGTGA	6 cut(s)	313, 319, 1127, 1558, 1820, 2057
AsuII	TTCGAA	1 cut(s)	43
AsuNHI	GCTAGC	2 cut(s)	79, 1255
AvaI	CYCGRG	1 cut(s)	1013
AvaII	GGWCC	2 cut(s)	350, 1868
AxyI	CCTNAGG	1 cut(s)	1872
BaeGI	GKGCMC	3 cut(s)	11, 623, 722
BanI	GGYRCC	2 cut(s)	719, 1133
BanII	GRGCYC	1 cut(s)	177
BbsI	GAAGAC	1 cut(s)	246
Bbv12I	GWGCWC	4 cut(s)	11, 177, 623, 1243
BbvI	GCAGC	7 cut(s)	463, 1210, 1313, 1648, 1975, 2075, 2078
BccI	CCATC	8 cut(s)	47, 257, 332, 603, 935, 955, 1028, 1931
BceAI	ACGGC	1 cut(s)	930
BciT130I	CCWGG	3 cut(s)	836, 1334, 1862
BclI	TGATCA	2 cut(s)	1077, 1261
BcnI	CCSGG	2 cut(s)	1014, 1015
BcoDI	GTCTC	3 cut(s)	452, 1181, 2102
BcuI	ACTAGT	2 cut(s)	806, 1420
BfaI	CTAG	7 cut(s)	80, 807, 857, 861, 932, 1256, 1421
BfmI	CTRYAG	4 cut(s)	591, 1400, 1461, 1662
BfrI	CTTAAG	1 cut(s)	1001
BglII	AGATCT	2 cut(s)	356, 1266
BisI	GCNGC	7 cut(s)	452, 1224, 1302, 1662, 1989, 2064, 2067
BlsI	GCNGC	7 cut(s)	453, 1225, 1303, 1663, 1990, 2065, 2068
BmcAI	AGTACT	2 cut(s)	845, 1419
Bme1390I	CCNGG	5 cut(s)	836, 1014, 1015, 1334, 1862
Bme18I	GGWCC	2 cut(s)	350, 1868
BmeT110I	CYCGRG	1 cut(s)	1013
BmgBI	CACGTC	1 cut(s)	304
BmgT120I	GGNCC	2 cut(s)	350, 1868
BmiI	GGNNCC	3 cut(s)	476, 721, 1135
BmrFI	CCNGG	5 cut(s)	836, 1014, 1015, 1334, 1862
BmrI	ACTGGG	1 cut(s)	725
BmsI	GCATC	9 cut(s)	145, 217, 325, 868, 925, 1142, 1419, 2040, 2097
BmtI	GCTAGC	2 cut(s)	83, 1259
BmuI	ACTGGG	1 cut(s)	725
BoxI	GACNNNNGTC	1 cut(s)	685
BpiI	GAAGAC	1 cut(s)	246
BpmI	CTGGAG	1 cut(s)	1803
Bpu10I	CCTNAGC	2 cut(s)	184, 1761
Bpu14I	TTCGAA	1 cut(s)	43
BpuEI	CTTGAG	3 cut(s)	107, 476, 508
BpuMI	CCSGG	2 cut(s)	1014, 1015
Bsa29I	ATCGAT	1 cut(s)	2091
BsaI	GGTCTC	1 cut(s)	2102
BsaJI	CCNNGG	6 cut(s)	428, 835, 1013, 1014, 1333, 2118
BsaXI	ACNNNNNCTCC	2 cut(s)	1776, 1806
Bsc4I	CCNNNNNNNGG	2 cut(s)	1034, 1362
Bse1I	ACTGG	9 cut(s)	12, 352, 478, 563, 720, 1786, 1911, 1973, 2047
Bse21I	CCTNAGG	1 cut(s)	1872
Bse3DI	GCAATG	2 cut(s)	762, 952
BseBI	CCWGG	3 cut(s)	836, 1334, 1862
BseCI	ATCGAT	1 cut(s)	2091
BseDI	CCNNGG	6 cut(s)	428, 835, 1013, 1014, 1333, 2118
BseGI	GGATG	4 cut(s)	252, 706, 1157, 1329
BseLI	CCNNNNNNNGG	2 cut(s)	1034, 1362
BseMI	GCAATG	2 cut(s)	762, 952
BseMII	CTCAG	7 cut(s)	162, 198, 476, 969, 1428, 1863, 2097
BseNI	ACTGG	9 cut(s)	12, 352, 478, 563, 720, 1786, 1911, 1973, 2047
BseSI	GKGCMC	3 cut(s)	11, 623, 722
BseXI	GCAGC	7 cut(s)	463, 1210, 1313, 1648, 1975, 2075, 2078
BseYI	CCCAGC	2 cut(s)	1318, 1949
BsgI	GTGCAG	2 cut(s)	650, 1209
BshFI	GGCC	1 cut(s)	1096
BshNI	GGYRCC	2 cut(s)	719, 1133
BshVI	ATCGAT	1 cut(s)	2091
BsiHKAI	GWGCWC	4 cut(s)	11, 177, 623, 1243
BsiHKCI	CYCGRG	1 cut(s)	1013
BsiSI	CCGG	1 cut(s)	1014
BslFI	GGGAC	2 cut(s)	569, 1588
BslI	CCNNNNNNNGG	2 cut(s)	1034, 1362
BsmAI	GTCTC	3 cut(s)	452, 1181, 2102
BsmFI	GGGAC	2 cut(s)	569, 1588
BsmI	GAATGC	1 cut(s)	603
BsnI	GGCC	1 cut(s)	1096
Bso31I	GGTCTC	1 cut(s)	2102
BsoBI	CYCGRG	1 cut(s)	1013
Bsp119I	TTCGAA	1 cut(s)	43
Bsp1286I	GDGCHC	5 cut(s)	11, 177, 623, 722, 1243
Bsp1407I	TGTACA	2 cut(s)	468, 567
Bsp19I	CCATGG	1 cut(s)	2118
BspACI	CCGC	1 cut(s)	1846
BspANI	GGCC	1 cut(s)	1096
BspCNI	CTCAG	7 cut(s)	163, 197, 475, 968, 1427, 1864, 2098
BspDI	ATCGAT	1 cut(s)	2091
BspHI	TCATGA	1 cut(s)	138
BspLI	GGNNCC	3 cut(s)	476, 721, 1135
BspMAI	CTGCAG	2 cut(s)	1404, 1666
BspOI	GCTAGC	2 cut(s)	83, 1259
BspPI	GGATC	6 cut(s)	104, 175, 670, 1744, 1964, 2108
BspQI	GCTCTTC	1 cut(s)	45
BspT104I	TTCGAA	1 cut(s)	43
BspT107I	GGYRCC	2 cut(s)	719, 1133
BspTI	CTTAAG	1 cut(s)	1001
BspTNI	GGTCTC	1 cut(s)	2102
BsrDI	GCAATG	2 cut(s)	762, 952
BsrGI	TGTACA	2 cut(s)	468, 567
BsrI	ACTGG	9 cut(s)	12, 352, 478, 563, 720, 1786, 1911, 1973, 2047
BssECI	CCNNGG	6 cut(s)	428, 835, 1013, 1014, 1333, 2118
BssT1I	CCWWGG	2 cut(s)	428, 2118
Bst2UI	CCWGG	3 cut(s)	836, 1334, 1862
Bst4CI	ACNGT	5 cut(s)	592, 1555, 1585, 1600, 1651
Bst6I	CTCTTC	3 cut(s)	45, 430, 1795
BstAFI	CTTAAG	1 cut(s)	1001
BstAPI	GCANNNNNTGC	2 cut(s)	15, 2072
BstAUI	TGTACA	2 cut(s)	468, 567
BstBI	TTCGAA	1 cut(s)	43
BstC8I	GCNNGC	8 cut(s)	81, 160, 412, 416, 1257, 1395, 1432, 1710
BstDEI	CTNAG	9 cut(s)	171, 184, 462, 847, 955, 1414, 1761, 1872, 2106
BstDSI	CCRYGG	1 cut(s)	2118
BstEII	GGTNACC	1 cut(s)	319
BstF5I	GGATG	4 cut(s)	252, 706, 1157, 1329
BstHHI	GCGC	1 cut(s)	1195
BstMAI	GTCTC	3 cut(s)	452, 1181, 2102
BstMWI	GCNNNNNNNGC	9 cut(s)	15, 538, 761, 1399, 1661, 1667, 2028, 2063, 2072
BstNI	CCWGG	3 cut(s)	836, 1334, 1862
BstNSI	RCATGY	7 cut(s)	422, 586, 1434, 1712, 1751, 1920, 1995
BstPAI	GACNNNNGTC	1 cut(s)	685
BstPI	GGTNACC	1 cut(s)	319
BstSCI	CCNGG	5 cut(s)	834, 1012, 1013, 1332, 1860
BstSFI	CTRYAG	4 cut(s)	591, 1400, 1461, 1662
BstSLI	GKGCMC	3 cut(s)	11, 623, 722
BstV1I	GCAGC	7 cut(s)	463, 1210, 1313, 1648, 1975, 2075, 2078
BstV2I	GAAGAC	1 cut(s)	246
BstX2I	RGATCY	3 cut(s)	167, 356, 1266
BstYI	RGATCY	3 cut(s)	167, 356, 1266
Bsu15I	ATCGAT	1 cut(s)	2091
Bsu36I	CCTNAGG	1 cut(s)	1872
BsuRI	GGCC	1 cut(s)	1096
BsuTUI	ATCGAT	1 cut(s)	2091
BtgI	CCRYGG	1 cut(s)	2118
BtrI	CACGTC	1 cut(s)	304
BtsCI	GGATG	4 cut(s)	252, 706, 1157, 1329
BtsI	GCAGTG	1 cut(s)	1397
BtsIMutI	CAGTG	8 cut(s)	120, 345, 588, 713, 1397, 1577, 1779, 1980
Cac8I	GCNNGC	8 cut(s)	81, 160, 412, 416, 1257, 1395, 1432, 1710
CaiI	CAGNNNCTG	3 cut(s)	509, 1190, 1988
CciI	TCATGA	1 cut(s)	138
CfoI	GCGC	1 cut(s)	1195
Cfr13I	GGNCC	2 cut(s)	350, 1868
Cfr9I	CCCGGG	1 cut(s)	1013
ClaI	ATCGAT	1 cut(s)	2091
Csp6I	GTAC	6 cut(s)	469, 568, 844, 1418, 1647, 1775
CviQI	GTAC	6 cut(s)	469, 568, 844, 1418, 1647, 1775
DdeI	CTNAG	9 cut(s)	171, 184, 462, 847, 955, 1414, 1761, 1872, 2106
DraIII	CACNNNGTG	1 cut(s)	1502
Eam1104I	CTCTTC	3 cut(s)	45, 430, 1795
EarI	CTCTTC	3 cut(s)	45, 430, 1795
Ecl136II	GAGCTC	1 cut(s)	175
Eco130I	CCWWGG	2 cut(s)	428, 2118
Eco147I	AGGCCT	1 cut(s)	1096
Eco24I	GRGCYC	1 cut(s)	177
Eco31I	GGTCTC	1 cut(s)	2102
Eco32I	GATATC	1 cut(s)	822
Eco47I	GGWCC	2 cut(s)	350, 1868
Eco53kI	GAGCTC	1 cut(s)	175
Eco57I	CTGAAG	1 cut(s)	258
Eco81I	CCTNAGG	1 cut(s)	1872
Eco88I	CYCGRG	1 cut(s)	1013
Eco91I	GGTNACC	1 cut(s)	319
EcoICRI	GAGCTC	1 cut(s)	175
EcoO65I	GGTNACC	1 cut(s)	319
EcoRI	GAATTC	2 cut(s)	1340, 1640
EcoRII	CCWGG	3 cut(s)	834, 1332, 1860
EcoRV	GATATC	1 cut(s)	822
EcoT14I	CCWWGG	2 cut(s)	428, 2118
EcoT38I	GRGCYC	1 cut(s)	177
ErhI	CCWWGG	2 cut(s)	428, 2118
FalI	AAGNNNNNCTT	2 cut(s)	89, 121
FaqI	GGGAC	2 cut(s)	569, 1588
FauI	CCCGC	1 cut(s)	1839
FbaI	TGATCA	2 cut(s)	1077, 1261
Fnu4HI	GCNGC	7 cut(s)	452, 1224, 1302, 1662, 1989, 2064, 2067
FokI	GGATG	4 cut(s)	239, 713, 1164, 1316
FriOI	GRGCYC	1 cut(s)	177
Fsp4HI	GCNGC	7 cut(s)	452, 1224, 1302, 1662, 1989, 2064, 2067
FspBI	CTAG	7 cut(s)	80, 807, 857, 861, 932, 1256, 1421
GlaI	GCGC	1 cut(s)	1194
GluI	GCNGC	7 cut(s)	452, 1224, 1302, 1662, 1989, 2064, 2067
GsaI	CCCAGC	2 cut(s)	1322, 1953
GsuI	CTGGAG	1 cut(s)	1803
HaeIII	GGCC	1 cut(s)	1096
HapII	CCGG	1 cut(s)	1014
HhaI	GCGC	1 cut(s)	1195
Hin6I	GCGC	1 cut(s)	1193
HinP1I	GCGC	1 cut(s)	1193
HindIII	AAGCTT	2 cut(s)	412, 2155
HinfI	GANTC	7 cut(s)	23, 32, 269, 460, 574, 613, 2136
HpaII	CCGG	1 cut(s)	1014
HphI	GGTGA	6 cut(s)	313, 319, 1127, 1558, 1820, 2057
Hpy166II	GTNNAC	8 cut(s)	9, 103, 199, 301, 621, 1066, 1679, 1777
Hpy188III	TCNNGA	6 cut(s)	139, 236, 487, 908, 1236, 1871
Hpy8I	GTNNAC	8 cut(s)	9, 103, 199, 301, 621, 1066, 1679, 1777
HpyAV	CCTTC	2 cut(s)	215, 1480
HpyCH4III	ACNGT	5 cut(s)	592, 1555, 1585, 1600, 1651
HpyCH4IV	ACGT	2 cut(s)	303, 682
HpyF10VI	GCNNNNNNNGC	9 cut(s)	15, 538, 761, 1399, 1661, 1667, 2028, 2063, 2072
HpyF3I	CTNAG	9 cut(s)	171, 184, 462, 847, 955, 1414, 1761, 1872, 2106
HpySE526I	ACGT	2 cut(s)	303, 682
HspAI	GCGC	1 cut(s)	1193
Ksp22I	TGATCA	2 cut(s)	1077, 1261
LguI	GCTCTTC	1 cut(s)	45
LmnI	GCTCC	3 cut(s)	23, 1705, 1784
Lsp1109I	GCAGC	7 cut(s)	463, 1210, 1313, 1648, 1975, 2075, 2078
LweI	GCATC	9 cut(s)	145, 217, 325, 868, 925, 1142, 1419, 2040, 2097
MaeI	CTAG	7 cut(s)	80, 807, 857, 861, 932, 1256, 1421
MaeII	ACGT	2 cut(s)	303, 682
MaeIII	GTNAC	6 cut(s)	319, 1115, 1808, 1963, 2038, 2145
MboII	GAAGA	7 cut(s)	32, 164, 251, 389, 447, 803, 1812
MfeI	CAATTG	1 cut(s)	2070
MflI	RGATCY	3 cut(s)	167, 356, 1266
MhlI	GDGCHC	5 cut(s)	11, 177, 623, 722, 1243
MlyI	GAGTC	2 cut(s)	32, 454
MmeI	TCCRAC	1 cut(s)	1567
MseI	TTAA	7 cut(s)	228, 563, 605, 677, 1002, 1164, 1725
MslI	CAYNNNNRTG	1 cut(s)	626
MspCI	CTTAAG	1 cut(s)	1001
MspI	CCGG	1 cut(s)	1014
MspR9I	CCNGG	5 cut(s)	836, 1014, 1015, 1334, 1862
MunI	CAATTG	1 cut(s)	2070
Mva1269I	GAATGC	1 cut(s)	603
MvaI	CCWGG	3 cut(s)	836, 1334, 1862
MwoI	GCNNNNNNNGC	9 cut(s)	15, 538, 761, 1399, 1661, 1667, 2028, 2063, 2072
NciI	CCSGG	2 cut(s)	1014, 1015
NcoI	CCATGG	1 cut(s)	2118
NheI	GCTAGC	2 cut(s)	79, 1255
NlaIV	GGNNCC	3 cut(s)	476, 721, 1135
NmuCI	GTSAC	3 cut(s)	319, 1115, 1808
NspI	RCATGY	7 cut(s)	422, 586, 1434, 1712, 1751, 1920, 1995
NspV	TTCGAA	1 cut(s)	43
OliI	CACNNNNGTG	1 cut(s)	626
PaeI	GCATGC	2 cut(s)	1434, 1712
PagI	TCATGA	1 cut(s)	138
PceI	AGGCCT	1 cut(s)	1096
PciI	ACATGT	2 cut(s)	1747, 1916
PciSI	GCTCTTC	1 cut(s)	45
PctI	GAATGC	1 cut(s)	603
PfeI	GAWTC	5 cut(s)	32, 269, 574, 613, 2136
PflMI	CCANNNNNTGG	1 cut(s)	1362
PkrI	GCNGC	7 cut(s)	453, 1225, 1303, 1663, 1990, 2065, 2068
PleI	GAGTC	2 cut(s)	31, 454
PpsI	GAGTC	2 cut(s)	31, 454
PscI	ACATGT	2 cut(s)	1747, 1916
PshAI	GACNNNNGTC	1 cut(s)	685
Psp124BI	GAGCTC	1 cut(s)	177
Psp6I	CCWGG	3 cut(s)	834, 1332, 1860
PspEI	GGTNACC	1 cut(s)	319
PspFI	CCCAGC	2 cut(s)	1318, 1949
PspGI	CCWGG	3 cut(s)	834, 1332, 1860
PspN4I	GGNNCC	3 cut(s)	476, 721, 1135
PspPI	GGNCC	2 cut(s)	350, 1868
PstI	CTGCAG	2 cut(s)	1404, 1666
PstNI	CAGNNNCTG	3 cut(s)	509, 1190, 1988
PsuI	RGATCY	3 cut(s)	167, 356, 1266
RsaI	GTAC	6 cut(s)	470, 569, 845, 1419, 1648, 1776
RsaNI	GTAC	6 cut(s)	469, 568, 844, 1418, 1647, 1775
RseI	CAYNNNNRTG	1 cut(s)	626
SacI	GAGCTC	1 cut(s)	177
SapI	GCTCTTC	1 cut(s)	45
SaqAI	TTAA	7 cut(s)	228, 563, 605, 677, 1002, 1164, 1725
SatI	GCNGC	7 cut(s)	452, 1224, 1302, 1662, 1989, 2064, 2067
Sau96I	GGNCC	2 cut(s)	350, 1868
ScaI	AGTACT	2 cut(s)	845, 1419
SchI	GAGTC	2 cut(s)	32, 454
ScrFI	CCNGG	5 cut(s)	836, 1014, 1015, 1334, 1862
SduI	GDGCHC	5 cut(s)	11, 177, 623, 722, 1243
SfaNI	GCATC	9 cut(s)	145, 217, 325, 868, 925, 1142, 1419, 2040, 2097
SfcI	CTRYAG	4 cut(s)	591, 1400, 1461, 1662
SfuI	TTCGAA	1 cut(s)	43
SinI	GGWCC	2 cut(s)	350, 1868
SmaI	CCCGGG	1 cut(s)	1015
SmiMI	CAYNNNNRTG	1 cut(s)	626
SmlI	CTYRAG	4 cut(s)	86, 455, 487, 1001
SmoI	CTYRAG	4 cut(s)	86, 455, 487, 1001
SpeI	ACTAGT	2 cut(s)	806, 1420
SphI	GCATGC	2 cut(s)	1434, 1712
SseBI	AGGCCT	1 cut(s)	1096
SsiI	CCGC	1 cut(s)	1846
SspMI	CTAG	7 cut(s)	80, 807, 857, 861, 932, 1256, 1421
SstI	GAGCTC	1 cut(s)	177
StuI	AGGCCT	1 cut(s)	1096
StyD4I	CCNGG	5 cut(s)	834, 1012, 1013, 1332, 1860
StyI	CCWWGG	2 cut(s)	428, 2118
TaaI	ACNGT	5 cut(s)	592, 1555, 1585, 1600, 1651
TaiI	ACGT	2 cut(s)	306, 685
TaqI	TCGA	4 cut(s)	43, 134, 1912, 2091
TaqII	GACCGA	2 cut(s)	1334, 2126
TatI	WGTACW	5 cut(s)	468, 567, 843, 1417, 1646
TfiI	GAWTC	5 cut(s)	32, 269, 574, 613, 2136
Tru1I	TTAA	7 cut(s)	228, 563, 605, 677, 1002, 1164, 1725
Tru9I	TTAA	7 cut(s)	228, 563, 605, 677, 1002, 1164, 1725
TscAI	CASTG	8 cut(s)	127, 352, 595, 720, 1404, 1584, 1786, 1980
TseFI	GTSAC	3 cut(s)	319, 1115, 1808
TseI	GCWGC	7 cut(s)	451, 1223, 1301, 1661, 1988, 2063, 2066
Tsp45I	GTSAC	3 cut(s)	319, 1115, 1808
TspDTI	ATGAA	5 cut(s)	155, 625, 773, 1154, 1318
TspGWI	ACGGA	1 cut(s)	16
TspMI	CCCGGG	1 cut(s)	1013
TspRI	CASTG	8 cut(s)	127, 352, 595, 720, 1404, 1584, 1786, 1980
Van91I	CCANNNNNTGG	1 cut(s)	1362
Vha464I	CTTAAG	1 cut(s)	1001
VneI	GTGCAC	2 cut(s)	7, 619
VpaK11BI	GGWCC	2 cut(s)	350, 1868
XapI	RAATTY	3 cut(s)	1340, 1640, 1883
XceI	RCATGY	7 cut(s)	422, 586, 1434, 1712, 1751, 1920, 1995
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1630
XmaI	CCCGGG	1 cut(s)	1013
XspI	CTAG	7 cut(s)	80, 807, 857, 861, 932, 1256, 1421
ZrmI	AGTACT	2 cut(s)	845, 1419

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.