Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

19373282 .. 19378264

Gene Structure 4983 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom11g17250.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGGATAATCTGAAGGAGCCTCTGTTGAAGCCTTTGGATATTGATAACAAAGATAAAAGAATTAGAACAGTGAAGTTTAAAATAGGGGATATCGAGTGTGCATCTTGTGCAACTACCATTGAATCTGTGTTGGGAAAGCTTGATGGGATCAAGAGTGCAACAGTGTCGCCCATTGAAGGCCAAGCTGCAGTCAATTATATACCAGAGCTCATTAATGCAAGAAAAATTAAGGAAGCTGTAGAAGATGCTGGTTTCCCTGTTTCTGAATTTCCAGAACAAGACGTAGCAGTATGCCGACTGAGGATAAAAGGAATGGCGTGCACCAGCTGCTCCGAGTCTGTTGAGTGCGCTCTTCGAATGGTCGATGGAGTCAAAAATGCAGTTGTTGGTCTAGCCCTTGAAGAAGCAAAGGTTCACTTTGATCCCAATCTCACTGATACTAGTTGCATTATTCAAGCAATTGAAGATGCTGGCTTTGGGGTTGAACTGGTTAGTTCTGGGAATGATGTCAGCAAACTTCATCTAAAACTTGAAGGAGTTGATTCTCCAGAGGATATGACTATTGTCCGATCCTCCGTTGAATCAGTTGAAGGAGTAAGTAATGTTGAAGTCGACTTGGCAGAGAAGAAGGTGACTATTACCTATGATTCAGACTTCACAGGTCCAAGATCTCTTATACGTTGTATTGAAGAAGCTGGCCTTGAATCCAAGGTGTACCAGGTAAGCTTGTATGTTCCTCCAAGACCAAGAGAAGCTGAGCGGAAACATGAGATTCAGATGTACAGGAACCAGTTTTTCCTTAGCTGCCTATTTTCCGTTCCTATATTTTTATTCTCAATGGTGCTTCCGATGCTTCCTCCTTATGGAAACTGGTTAGACTACAAGGTTCACAATATGCTCACTGTAGGGATGCTTTTAAGATGGATTCTTTGCACACCAGTGCAGTTCATTGTTGGCCGGAGGTTCTATGTTGGATCATATCATGCGTTGAGACGTAGATCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTTTACTCTGTCTATATTGCAATAAAAGCATTTGCTTTGGATAAATTTGAAGGGCAAGATTTCTTTGAAACTAGCTCTATGTTGATATCCTTTATACTCTTGGGAAAGTACTTAGAAGTTCTGGCTAAAGGGAAGACATCTGATGCTTTGGCGAAGCTGACAGACCTTGCTCCTGATACGGCTTTTCTGTTGTCATTAGATGACGATGACAATGTTATCTCAGAGATCGAAATCAGCACTCAACTTATTCAAAAAAATGATATACTTAAGGTTGCTCCGGGGGCAAAAGTTCCTGTTGATGGGATTGTAGTTCGTGGTCACAGCTATGTGAATGAGAGTATGATCACAGGAGAGGCAACACCCATTTCTAAAAAACTTGGTGACAAGGTAATTGGTGGCACTATGAATGAGAATGGATGCTTACTGGTGAAGGCAACTCATGTTGGTTCGGAGACTGCACTTTCCCAAATAGTTCAACTTGTTGAAGCTGCACAGCTTGCCAGAGCACCTGTTCAGAAACTAGCTGACCAGATTTCAAAGTTTTTTGTTCCAACGGTTGTCATTGTAGCATTCTTGACATGGCTTGGATGGTTCATCCCTGGAGAATTTGGTCTTTTCCCAGAAAACTGGATACCAAAAGGCATGGATAAATTTGAGCTGGCATTACAATTTGGTATCTCAGTATTAGTGGTTGCTTGCCCTTGTGCCTTGGGACTAGCAACCCCTACGGCAGTAATGGTTGCAACAGGGAAGGGTGCTTCGCAAGGTGTTCTTATCAAGGGTGGAAATGCACTCGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAAACAGGAACTCTGACAGTTGGGAAACCCGTGGTAGTCAATGCAGTGCTCTTCTCCAGTTACTCAATGGAGGAGTTCTGTGCTGTCTCTATTGCCACAGAGGCAAATAGTGAACACCCAATAGCGAAATCGGTGGTGGAGCATGCTAAGAGGCTGCTAAAGACGTTTGGATCAACTGAACATGTGATGGAGGCTAAGGATTTTGAGGTGCACACAGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGACTCATGCGGCATAATAATGTCCAGGTTGGTTCTGAGGTTGAGAAATATGTTTCAGAACACGAGAATCTGGCTCAAACATGTGTGTTGGTTGCCGTTGATGGAAAGATTGCCGGGTCGTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCGCGGGTCATCTCTTATCTTCACTCGATGAACATTACTAGCATCATGGTTACTGGTGATAACTGGGCTACAGCAGCAGCCATCGCAAAGGAGGTTGGCATTGATAAGGTGTATGCTGAGACGGATCCACTGGGAAAAGCTGATAGAATCAAAGAATTGCAGATGAAAGGAATGGCGGTGGCAATGGTGGGAGATGGAATCAATGACTCTCCAGCTTTGGTTGCAGCAGATGTTGGCATGGCAATTGGTGCTGGCACCGACGTCGCTATAGAAGCCGCTGATATAGTTCTGATGAAGAGCAACTTGGAAGATGTAGTTACAGCCATAGATGATCGATCCAGAAAAACTATGTCACGAATTCGATTGAATTATGTCTGGGCCCTGGGCTACAATATCCTCGGCATGCCAGTCGCAGCTGGAGTCTTGTTCCCTTTCACTGGAATTCGACTACCACCGTGGCTTGCCGGTGCATGTATGGCTGCTTCATCTCTCAGCGTGGTCTGTTCTTCTCTCTTGTTGCAGTCATATAAGAAGCCTTTGCATATTCAGAGCGTCTCCAACGGCAAGTTTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

959

Amino Acids

103.61

Weight (kDa)

5.68

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2565
AccB1I	GGYRCC	2 cut(s)	1031, 2555
AccBSI	CCGCTC	1 cut(s)	760
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	1 cut(s)	2305
AciI	CCGC	5 cut(s)	760, 2158, 2305, 2477, 2577
AclWI	GGATC	9 cut(s)	155, 416, 564, 982, 2056, 2276, 2420, 2433, 2631
AcoI	YGGCCR	1 cut(s)	955
AcsI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2658, 2742
AcuI	CTGAAG	1 cut(s)	32
AcyI	GRCGYC	1 cut(s)	2562
AfaI	GTAC	3 cut(s)	716, 782, 1157
AfiI	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
AflII	CTTAAG	1 cut(s)	1313
AflIII	ACRYGT	2 cut(s)	2059, 2228
AhlI	ACTAGT	1 cut(s)	440
AjnI	CCWGG	4 cut(s)	717, 1645, 2172, 2682
AjuI	GAANNNNNNNTTGG	2 cut(s)	2588, 2620
AleI	CACNNNNGTG	1 cut(s)	938
Alw21I	GWGCWC	5 cut(s)	210, 323, 1555, 1929, 2091
Alw26I	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
Alw44I	GTGCAC	2 cut(s)	319, 2087
AlwI	GGATC	9 cut(s)	155, 416, 564, 982, 2056, 2276, 2420, 2433, 2631
AlwNI	CAGNNNCTG	2 cut(s)	2099, 2300
AoxI	GGCC	4 cut(s)	178, 697, 955, 2679
ApaI	GGGCCC	1 cut(s)	2683
ApaLI	GTGCAC	2 cut(s)	319, 2087
ApoI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2658, 2742
AseI	ATTAAT	1 cut(s)	213
AspLEI	GCGC	2 cut(s)	350, 2305
AspS9I	GGNCC	3 cut(s)	662, 2679, 2680
AsuC2I	CCSGG	2 cut(s)	1326, 2263
AsuHPI	GGTGA	6 cut(s)	643, 1439, 1486, 1870, 2132, 2369
AsuII	TTCGAA	1 cut(s)	355
AvaII	GGWCC	1 cut(s)	662
BaeGI	GKGCMC	4 cut(s)	323, 1034, 2091, 2683
BamHI	GGATCC	1 cut(s)	2425
BanI	GGYRCC	2 cut(s)	1031, 2555
BanII	GRGCYC	2 cut(s)	210, 2683
BarI	GAAGNNNNNNTAC	2 cut(s)	2767, 2799
BauI	CACGAG	1 cut(s)	2210
BbsI	GAAGAC	1 cut(s)	1187
Bbv12I	GWGCWC	5 cut(s)	210, 323, 1555, 1929, 2091
BccI	CCATC	9 cut(s)	137, 359, 915, 1340, 1629, 2059, 2243, 2390, 2489
BceAI	ACGGC	3 cut(s)	1242, 1791, 2228
BcgI	CGANNNNNNTGC	6 cut(s)	147, 181, 2545, 2579, 2692, 2726
BciT130I	CCWGG	4 cut(s)	719, 1647, 2174, 2684
BciVI	GTATCC	1 cut(s)	1671
BclI	TGATCA	1 cut(s)	1389
BcnI	CCSGG	2 cut(s)	1326, 2263
BcoDI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
BcuI	ACTAGT	1 cut(s)	440
BfaI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
BfmI	CTRYAG	5 cut(s)	186, 237, 903, 2370, 2568
BfrI	CTTAAG	1 cut(s)	1313
BfuI	GTATCC	1 cut(s)	1671
BglI	GCCNNNNNGGC	1 cut(s)	1979
BglII	AGATCT	1 cut(s)	668
BlpI	GCTNAGC	1 cut(s)	756
BmcAI	AGTACT	1 cut(s)	1157
Bme1390I	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
Bme18I	GGWCC	1 cut(s)	662
BmgT120I	GGNCC	3 cut(s)	662, 2679, 2680
BmiI	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
BmrFI	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
BmrI	ACTGGG	3 cut(s)	1037, 2374, 2441
BmsI	GCATC	8 cut(s)	110, 235, 457, 840, 900, 1180, 1454, 2352
BmuI	ACTGGG	3 cut(s)	1037, 2374, 2441
BoxI	GACNNNNGTC	1 cut(s)	563
BpiI	GAAGAC	1 cut(s)	1187
BpmI	CTGGAG	5 cut(s)	531, 1668, 1918, 2496, 2739
Bpu10I	CCTNAGC	2 cut(s)	800, 2073
Bpu1102I	GCTNAGC	1 cut(s)	756
Bpu14I	TTCGAA	1 cut(s)	355
BpuMI	CCSGG	2 cut(s)	1326, 2263
Bsa29I	ATCGAT	1 cut(s)	2635
BsaBI	GATNNNNATC	2 cut(s)	426, 1277
BsaHI	GRCGYC	1 cut(s)	2562
BsaJI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2682, 2683, 2698, 2756
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 2088, 2118
Bsc4I	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
Bse118I	RCCGGY	1 cut(s)	2765
Bse3DI	GCAATG	1 cut(s)	2490
Bse8I	GATNNNNATC	2 cut(s)	426, 1277
BseBI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
BseCI	ATCGAT	1 cut(s)	2635
BseDI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2682, 2683, 2698, 2756
BseGI	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BseJI	GATNNNNATC	2 cut(s)	426, 1277
BseLI	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
BseMI	GCAATG	1 cut(s)	2490
BseMII	CTCAG	7 cut(s)	290, 747, 1281, 1740, 2175, 2409, 2806
BseRI	GAGGAG	1 cut(s)	1964
BseSI	GKGCMC	4 cut(s)	323, 1034, 2091, 2683
BsgI	GTGCAG	3 cut(s)	962, 1488, 1521
Bsh1236I	CGCG	1 cut(s)	2305
BshFI	GGCC	4 cut(s)	180, 699, 957, 2681
BshNI	GGYRCC	2 cut(s)	1031, 2555
BshVI	ATCGAT	1 cut(s)	2635
BsiHKAI	GWGCWC	5 cut(s)	210, 323, 1555, 1929, 2091
BsiSI	CCGG	4 cut(s)	958, 1325, 2262, 2766
BslFI	GGGAC	1 cut(s)	1773
BslI	CCNNNNNNNGG	4 cut(s)	176, 863, 1346, 2738
BsmAI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
BsmBI	CGTCTC	3 cut(s)	985, 2414, 2860
BsmFI	GGGAC	1 cut(s)	1773
BsmI	GAATGC	1 cut(s)	1616
BsnI	GGCC	4 cut(s)	180, 699, 957, 2681
Bsp119I	TTCGAA	1 cut(s)	355
Bsp120I	GGGCCC	1 cut(s)	2679
Bsp1286I	GDGCHC	7 cut(s)	210, 323, 1034, 1555, 1929, 2091, 2683
Bsp1407I	TGTACA	1 cut(s)	780
Bsp1720I	GCTNAGC	1 cut(s)	756
BspACI	CCGC	5 cut(s)	760, 2158, 2305, 2477, 2577
BspANI	GGCC	4 cut(s)	180, 699, 957, 2681
BspCNI	CTCAG	7 cut(s)	291, 748, 1280, 1739, 2176, 2410, 2805
BspDI	ATCGAT	1 cut(s)	2635
BspFNI	CGCG	1 cut(s)	2305
BspLI	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
BspMAI	CTGCAG	1 cut(s)	190
BspPI	GGATC	9 cut(s)	155, 416, 564, 982, 2056, 2276, 2420, 2433, 2631
BspQI	GCTCTTC	3 cut(s)	357, 1934, 2591
BspT104I	TTCGAA	1 cut(s)	355
BspT107I	GGYRCC	2 cut(s)	1031, 2555
BspTI	CTTAAG	1 cut(s)	1313
BsrBI	CCGCTC	1 cut(s)	760
BsrDI	GCAATG	1 cut(s)	2490
BsrFI	RCCGGY	1 cut(s)	2765
BsrGI	TGTACA	1 cut(s)	780
BssAI	RCCGGY	1 cut(s)	2765
BssECI	CCNNGG	9 cut(s)	708, 1325, 1645, 1755, 1908, 2682, 2683, 2698, 2756
BssNI	GRCGYC	1 cut(s)	2562
BssSI	CACGAG	1 cut(s)	2210
BssT1I	CCWWGG	2 cut(s)	708, 1755
Bst2BI	CACGAG	1 cut(s)	2210
Bst2UI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
Bst4CI	ACNGT	7 cut(s)	70, 163, 904, 1603, 1867, 1897, 2757
Bst6I	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
BstACI	GRCGYC	1 cut(s)	2562
BstAFI	CTTAAG	1 cut(s)	1313
BstAPI	GCANNNNNTGC	4 cut(s)	107, 327, 1544, 2549
BstAUI	TGTACA	1 cut(s)	780
BstBI	TTCGAA	1 cut(s)	355
BstDSI	CCRYGG	2 cut(s)	1908, 2756
BstF5I	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BstFNI	CGCG	1 cut(s)	2305
BstHHI	GCGC	2 cut(s)	350, 2305
BstMAI	GTCTC	6 cut(s)	985, 1493, 1969, 2143, 2414, 2860
BstNI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
BstNSI	RCATGY	5 cut(s)	2024, 2063, 2232, 2707, 2775
BstPAI	GACNNNNGTC	1 cut(s)	563
BstSCI	CCNGG	6 cut(s)	717, 1324, 1645, 2172, 2261, 2682
BstSFI	CTRYAG	5 cut(s)	186, 237, 903, 2370, 2568
BstSLI	GKGCMC	4 cut(s)	323, 1034, 2091, 2683
BstUI	CGCG	1 cut(s)	2305
BstV2I	GAAGAC	1 cut(s)	1187
BstX2I	RGATCY	2 cut(s)	668, 2425
BstXI	CCANNNNNNTGG	1 cut(s)	1674
BstYI	RGATCY	2 cut(s)	668, 2425
Bsu15I	ATCGAT	1 cut(s)	2635
BsuI	GTATCC	1 cut(s)	1671
BsuRI	GGCC	4 cut(s)	180, 699, 957, 2681
BsuTUI	ATCGAT	1 cut(s)	2635
BtgI	CCRYGG	2 cut(s)	1908, 2756
BtgZI	GCGATG	1 cut(s)	2368
BtsCI	GGATG	5 cut(s)	915, 1018, 1469, 1640, 1641
BtsI	GCAGTG	1 cut(s)	1929
CaiI	CAGNNNCTG	2 cut(s)	2099, 2300
CfoI	GCGC	2 cut(s)	350, 2305
Cfr10I	RCCGGY	1 cut(s)	2765
Cfr13I	GGNCC	3 cut(s)	662, 2679, 2680
ClaI	ATCGAT	1 cut(s)	2635
CseI	GACGC	1 cut(s)	2842
CsiI	ACCWGGT	1 cut(s)	717
Csp6I	GTAC	3 cut(s)	715, 781, 1156
CviQI	GTAC	3 cut(s)	715, 781, 1156
DraI	TTTAAA	1 cut(s)	79
EaeI	YGGCCR	1 cut(s)	955
Eam1104I	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
EarI	CTCTTC	4 cut(s)	357, 1934, 2107, 2591
Ecl136II	GAGCTC	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	708, 1755
Eco24I	GRGCYC	2 cut(s)	210, 2683
Eco32I	GATATC	2 cut(s)	91, 1134
Eco47I	GGWCC	1 cut(s)	662
Eco53kI	GAGCTC	1 cut(s)	208
Eco57I	CTGAAG	1 cut(s)	32
EcoICRI	GAGCTC	1 cut(s)	208
EcoO109I	RGGNCCY	1 cut(s)	2680
EcoRI	GAATTC	2 cut(s)	2658, 2742
EcoRII	CCWGG	4 cut(s)	717, 1645, 2172, 2682
EcoRV	GATATC	2 cut(s)	91, 1134
EcoT14I	CCWWGG	2 cut(s)	708, 1755
EcoT38I	GRGCYC	2 cut(s)	210, 2683
ErhI	CCWWGG	2 cut(s)	708, 1755
Esp3I	CGTCTC	3 cut(s)	985, 2414, 2860
FalI	AAGNNNNNCTT	8 cut(s)	401, 433, 684, 716, 1803, 1835, 2588, 2620
FaqI	GGGAC	1 cut(s)	1773
FauI	CCCGC	1 cut(s)	2298
FbaI	TGATCA	1 cut(s)	1389
FblI	GTMKAC	1 cut(s)	612
FokI	GGATG	5 cut(s)	922, 1025, 1476, 1628, 1647
FriOI	GRGCYC	2 cut(s)	210, 2683
FspBI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
GlaI	GCGC	2 cut(s)	349, 2304
GsuI	CTGGAG	5 cut(s)	531, 1668, 1918, 2496, 2739
HaeIII	GGCC	4 cut(s)	180, 699, 957, 2681
HapII	CCGG	4 cut(s)	958, 1325, 2262, 2766
HgaI	GACGC	1 cut(s)	2842
HhaI	GCGC	2 cut(s)	350, 2305
Hin1I	GRCGYC	1 cut(s)	2562
Hin6I	GCGC	2 cut(s)	348, 2303
HinP1I	GCGC	2 cut(s)	348, 2303
HincII	GTYRAC	1 cut(s)	613
HindII	GTYRAC	1 cut(s)	613
HindIII	AAGCTT	2 cut(s)	137, 724
HpaII	CCGG	4 cut(s)	958, 1325, 2262, 2766
HphI	GGTGA	6 cut(s)	643, 1439, 1486, 1870, 2132, 2369
Hpy166II	GTNNAC	7 cut(s)	321, 415, 613, 715, 889, 1991, 2089
Hpy188III	TCNNGA	7 cut(s)	151, 272, 548, 1220, 1621, 2640, 2655
Hpy8I	GTNNAC	7 cut(s)	321, 415, 613, 715, 889, 1991, 2089
Hpy99I	CGWCG	2 cut(s)	2564, 2567
HpyAV	CCTTC	8 cut(s)	7, 170, 527, 584, 622, 1091, 1471, 1792
HpyCH4III	ACNGT	7 cut(s)	70, 163, 904, 1603, 1867, 1897, 2757
HpyCH4IV	ACGT	5 cut(s)	282, 679, 994, 2042, 2562
HpySE526I	ACGT	5 cut(s)	282, 679, 994, 2042, 2562
Hsp92I	GRCGYC	1 cut(s)	2562
HspAI	GCGC	2 cut(s)	348, 2303
Ksp22I	TGATCA	1 cut(s)	1389
LguI	GCTCTTC	3 cut(s)	357, 1934, 2591
LmnI	GCTCC	6 cut(s)	16, 335, 1222, 1327, 2017, 2096
LweI	GCATC	8 cut(s)	110, 235, 457, 840, 900, 1180, 1454, 2352
MabI	ACCWGGT	1 cut(s)	717
MaeI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
MaeII	ACGT	5 cut(s)	282, 679, 994, 2042, 2562
MaeIII	GTNAC	9 cut(s)	631, 1364, 1427, 1937, 2120, 2275, 2350, 2617, 2652
MbiI	CCGCTC	1 cut(s)	760
MfeI	CAATTG	2 cut(s)	459, 2544
MflI	RGATCY	2 cut(s)	668, 2425
MhlI	GDGCHC	7 cut(s)	210, 323, 1034, 1555, 1929, 2091, 2683
MlyI	GAGTC	5 cut(s)	344, 378, 2145, 2501, 2730
MmeI	TCCRAC	2 cut(s)	952, 1622
MseI	TTAA	5 cut(s)	78, 213, 228, 917, 1314
MslI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
MspA1I	CMGCKG	3 cut(s)	327, 2579, 2717
MspCI	CTTAAG	1 cut(s)	1313
MspI	CCGG	4 cut(s)	958, 1325, 2262, 2766
MspR9I	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
MunI	CAATTG	2 cut(s)	459, 2544
Mva1269I	GAATGC	1 cut(s)	1616
MvaI	CCWGG	4 cut(s)	719, 1647, 2174, 2684
MvnI	CGCG	1 cut(s)	2305
NciI	CCSGG	2 cut(s)	1326, 2263
NlaIV	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
NmeAIII	GCCGAG	1 cut(s)	2679
NmuCI	GTSAC	5 cut(s)	631, 1364, 1427, 2120, 2652
NspI	RCATGY	5 cut(s)	2024, 2063, 2232, 2707, 2775
NspV	TTCGAA	1 cut(s)	355
OliI	CACNNNNGTG	1 cut(s)	938
PaeI	GCATGC	2 cut(s)	2024, 2707
PasI	CCCWGGG	1 cut(s)	2683
PciI	ACATGT	2 cut(s)	2059, 2228
PciSI	GCTCTTC	3 cut(s)	357, 1934, 2591
PctI	GAATGC	1 cut(s)	1616
PleI	GAGTC	5 cut(s)	343, 377, 2145, 2501, 2729
PpsI	GAGTC	5 cut(s)	343, 377, 2145, 2501, 2729
PscI	ACATGT	2 cut(s)	2059, 2228
PshAI	GACNNNNGTC	1 cut(s)	563
PshBI	ATTAAT	1 cut(s)	213
Psp124BI	GAGCTC	1 cut(s)	210
Psp6I	CCWGG	4 cut(s)	717, 1645, 2172, 2682
PspGI	CCWGG	4 cut(s)	717, 1645, 2172, 2682
PspN4I	GGNNCC	6 cut(s)	18, 788, 1033, 2427, 2557, 2681
PspOMI	GGGCCC	1 cut(s)	2679
PspPI	GGNCC	3 cut(s)	662, 2679, 2680
PstI	CTGCAG	1 cut(s)	190
PstNI	CAGNNNCTG	2 cut(s)	2099, 2300
PsuI	RGATCY	2 cut(s)	668, 2425
PvuII	CAGCTG	2 cut(s)	327, 2717
RsaI	GTAC	3 cut(s)	716, 782, 1157
RsaNI	GTAC	3 cut(s)	715, 781, 1156
RseI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
SacI	GAGCTC	1 cut(s)	210
SalI	GTCGAC	1 cut(s)	611
SapI	GCTCTTC	3 cut(s)	357, 1934, 2591
SaqAI	TTAA	5 cut(s)	78, 213, 228, 917, 1314
Sau96I	GGNCC	3 cut(s)	662, 2679, 2680
ScaI	AGTACT	1 cut(s)	1157
SchI	GAGTC	5 cut(s)	344, 378, 2145, 2501, 2730
ScrFI	CCNGG	6 cut(s)	719, 1326, 1647, 2174, 2263, 2684
SduI	GDGCHC	7 cut(s)	210, 323, 1034, 1555, 1929, 2091, 2683
SexAI	ACCWGGT	1 cut(s)	717
SfaNI	GCATC	8 cut(s)	110, 235, 457, 840, 900, 1180, 1454, 2352
SfcI	CTRYAG	5 cut(s)	186, 237, 903, 2370, 2568
SfuI	TTCGAA	1 cut(s)	355
SinI	GGWCC	1 cut(s)	662
SmiMI	CAYNNNNRTG	3 cut(s)	938, 1371, 2166
SmlI	CTYRAG	1 cut(s)	1313
SmoI	CTYRAG	1 cut(s)	1313
SpeI	ACTAGT	1 cut(s)	440
SphI	GCATGC	2 cut(s)	2024, 2707
SsiI	CCGC	5 cut(s)	760, 2158, 2305, 2477, 2577
SspMI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
SstI	GAGCTC	1 cut(s)	210
StyD4I	CCNGG	6 cut(s)	717, 1324, 1645, 2172, 2261, 2682
StyI	CCWWGG	2 cut(s)	708, 1755
TaaI	ACNGT	7 cut(s)	70, 163, 904, 1603, 1867, 1897, 2757
TaiI	ACGT	5 cut(s)	285, 682, 997, 2045, 2565
TatI	WGTACW	2 cut(s)	780, 1155
TauI	GCSGC	2 cut(s)	2161, 2579
Tru1I	TTAA	5 cut(s)	78, 213, 228, 917, 1314
Tru9I	TTAA	5 cut(s)	78, 213, 228, 917, 1314
TseFI	GTSAC	5 cut(s)	631, 1364, 1427, 2120, 2652
Tsp45I	GTSAC	5 cut(s)	631, 1364, 1427, 2120, 2652
TspDTI	ATGAA	8 cut(s)	509, 937, 1466, 1630, 2345, 2480, 2609, 2775
TspGWI	ACGGA	3 cut(s)	565, 805, 2438
Vha464I	CTTAAG	1 cut(s)	1313
VneI	GTGCAC	2 cut(s)	319, 2087
VpaK11BI	GGWCC	1 cut(s)	662
VspI	ATTAAT	1 cut(s)	213
XapI	RAATTY	6 cut(s)	266, 1091, 1652, 1697, 2658, 2742
XceI	RCATGY	5 cut(s)	2024, 2063, 2232, 2707, 2775
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1942
XmiI	GTMKAC	1 cut(s)	612
XspI	CTAG	6 cut(s)	392, 441, 1119, 1568, 1763, 2340
ZraI	GACGTC	1 cut(s)	2563
ZrmI	AGTACT	1 cut(s)	1157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.