Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

56617806 .. 56630250

Gene Structure 12445 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034888

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3180 bp

ATGGTGGATGGGATTATTGGCGGCACCAGCGATATGGCTAGCGATGGTATTGGCACCCGAGTCGATGGCTTGGCTGAACGGATCCTCAATCCAGAGAGGCTGCTAGCATCTTCCGGCTTCGTCACCGTCACCGTCACGTCGTCTTCATCATCCGGTAAAGGTTTGATGGATTCAAACCATTTGAATGGCGGAGTGGATGCTAATGGGATGGATGATATCAACCAGCCTCTGTTGGAGCACTTGGATGTTAGAGCTGCAGATAAGAGAATTAGAACACTTAAGTTCAAAATTGGGGAAATCCACTGTGCTTCTTGTTCAAATTCCATTGAATCTGTGGTGGGAAGCCTTAATGGGGTCAAGAGCGTTACGGTGTCACCCATTCATGGCCAAGCTGCTATCGATTATATACCAGAGCTCATTAATGGAAGCAAAATCAAGGAAGCCATAGAAGATGCAGGTTTCCCAGTTGTTGAATTTCCAGAACAAGACATTGCAGTATGCCGGCTCAGGATAAAAGGAATGATGTGCACCAGTTGCTCTGAGTCTGTTGAATCTGCTCTTCGAATGGTTGATGGAGTGAAAAACGCAGTTGTTGGTCTAGCCCTTGAGGAAGCAAAGGTTCACTTTGATCCAAACATCACTGACACCTGTTCGATCATGAATGCAATAGAAGATGCTGGCTTTGGATCTGAGCTCATTAGTTCAGGGAATGATGTAAACAAAGTGCATCTAAAACTTGAAGGAGTTAATTCTCCTGAAGACATGACCATCATCCAATCCTCCCTTGAATCAGTTGAGGGTGTAAATAATGTTGAAGTGGACGTGTTAGAAAAAAAGGTCACTATTACCTACGATGCAGACATCACCGGTCCAAGATCTCTTATACATTGTATTGAAGAAGCTGGAAGTAACCCCAAATCATACCAAGCAAGCTTGTATGTTCCTCCAAGACGAAGAGAAGCTGAACAGCAGCTTGAGACTCAGATGTACAGGAACCAGTTTTTCTTGAGCTGTCTATTTTCAGTTCCTGTATTTTTATTCTCAATGGTGCTTCCAATGCTTTCTCCCTATGGGGACTGGTTAATGTACAAGATTCACAACATGCTCACTGTAGGAATGCTTTTAAGATGGATTCTGTGCACACCCGTGCAGTTCATTATTGGCAGGAGGTTTTATGTGGGATCATATCATGCTTTAAGACGTAGGTCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTCTACTCTGTCTATATAGCAATGAAAGCCTTGGCTTTGGATAATTTTGAGGGGGAAGATTTCTTTGAAACTAGTTCCATGTTGATATCCTTTATACTCTTGGGGAAGTACTTAGAAGTTCTAGCTAGAGGAAAAACATCTGATGCTTTAGCGAAGCTGACAGACCTTACTCCTGAAACGGCTTATCTGCTATCACTAGATGCTGATGGCAATGCTATCTCAGAGATGGAAATTAGCACTCAACTTATTCAAAGAAATGATATACTTAAGATTGTTCCCGGGGCAAAAGTTCCTGTTGATGGGATTGTAATTAGTGGTCAAAGCCATGTGAACGAGAGTATGATCACAGGAGAGGCAAGGCCTATTTCTAAAAGATTAGGTGACAAGGTAATTGGTGGCACCATGAACGAGAATGGATGCTTACAGGTTAAGGCAACCCATGTTGGTTCAGAGACTGCGCTATCCCAGATAGTTCAACTTGTTGAAGCTGCACAGCTTTCCAGAGCACCTGTTCAGAAGCTAGCTGATCAGATCTCAAAGTTTTTTGTTCCAACAGTTGTGATTGCAGCATTTCTGACATGGCTGGGTTGGTTCATCCTGGGGGAATTTGGTCTTTATCCCATATCATGGACACCAAAAGGCATGGATAGGTTTGAGCTGGCACTGCAGTTCGGCATCTCAGTACTAGTGGTTGCATGCCCTTGTGCTTTGGGATTAGCAACACCTACAGCAGTCATGGTTGCAACTGGGAAGGGTGCTACACAAGGTGTTCTTATCAAGGGTGGAAATGCACTTGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAGACAGGGACACTGACAGTTGGAAAACCGACAGTAGTTAGTGCTGTGCTATTTTCCAATTACTCAATGGAGGAATTCTGTACTGTGGCTACTGCTGCAGAGGCAAATAGTGAACACCCAATAGCAAAGTCTATTGTGGAGCATGCAAAGAGGTTTCTTAACAAGTTTGGGTCAACTGAACATGTGGTGGAGGCTAAGGACTTTGAGGTACACACTGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGGCTCATGCGGGAATATAATGTCCAGGTTGGTCCTGAGGTTGAGAAATTCGTTTCAGAAAATGAGAAACTGGCTCGAACATGTGTGTTAGTTTCCATTGATGGAAAGGTTGCTGGGTCATTTGCTGTAACAGATCCAGTGAAGCCAGAGGCTGCATGTGTAATATCTTATCTCCACTCAATGGGCATTTCAAGCATCATGGTTACTGGTGATAATTGGGCAACAGCAGCAGCAATTGCAAGGGAGGTTGGCATCGATAAGGTGTTTGCTGAGACCGATCCCATGGGAAAAGCTGATAGAATCAAAGAGTTACAGATGAAAGGATTGACTGTGACTATGGTGGGAGATGGAATCAATGACTCACCAGCTTTGGCTGCAGCAGATGTTGGCATGGCAATTGGCGCTGGCACCGATGTAGCAATAGAAGCTGCTGATATAGTTTTGATGAAGAGCAATTTGGAAGATGTAGTTACAGCCATAGATCTCTCTAGAAAGACTATGTCTCGAATTTGGTTGAATTATGTCTGGGCCCTGGGATACAACATCCTTGGCATGCCAGTTGCTGCGGGAATCTTGTTCCCTTTCACTGGGATCCGATTACCACCTTGGCTTGCCGGTGCCTGTATGGCTGCTTCATCTGTTAGTGTGTTGGCCTCTACTCTTCTCGCTCTCGCCGACACTGATGAGACTCTTGATGTTCAGCATGCAACCAGAAGTCTGTCAACAAATGGTGAGTTATCGACTCCGGCAAAGACTTTTGACAATCAAGAAGCGCATATTCTGGGCAAGGCTAATAACATTTTCGACTCCGGCAAAGACTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1060

Amino Acids

113.75

Weight (kDa)

5.19

Isoelectric Point (pI)

33.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	95 - 154	2.8e-12	Heavy-metal-associated domain
HMA	PF00403	169 - 228	2.4e-08	Heavy-metal-associated domain
HMA	PF00403	258 - 302	3.1e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	476 - 580	8.7e-25	P-type ATPase actuator domain
Hydrolase	PF00702	689 - 913	1.6e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	446
AccB1I	GGYRCC	6 cut(s)	23, 53, 1238, 1652, 2762, 2972
AccB7I	CCANNNNNTGG	2 cut(s)	1881, 2536
AciI	CCGC	4 cut(s)	21, 189, 2365, 2921
AclWI	GGATC	9 cut(s)	76, 89, 623, 694, 1189, 2483, 2627, 2941, 2954
AcoI	YGGCCR	1 cut(s)	385
AcsI	RAATTY	6 cut(s)	319, 473, 1859, 2159, 2402, 2862
AcuI	CTGAAG	1 cut(s)	777
AdeI	CACNNNGTG	1 cut(s)	2021
AfaI	GTAC	6 cut(s)	989, 1088, 1364, 1938, 2167, 2295
AfiI	CCNNNNNNNGG	6 cut(s)	352, 383, 1553, 1881, 2536, 2942
AflII	CTTAAG	2 cut(s)	278, 1520
AflIII	ACRYGT	3 cut(s)	822, 2266, 2435
AgeI	ACCGGT	1 cut(s)	866
AhlI	ACTAGT	2 cut(s)	1325, 1939
AjiI	CACGTC	2 cut(s)	138, 823
AjnI	CCWGG	3 cut(s)	1851, 2379, 2886
AjuI	GAANNNNNNNTTGG	2 cut(s)	2795, 2827
AleI	CACNNNNGTG	1 cut(s)	1145
Alw21I	GWGCWC	6 cut(s)	240, 417, 530, 696, 1142, 1762
Alw26I	GTCTC	5 cut(s)	971, 1700, 2621, 2862, 3035
Alw44I	GTGCAC	2 cut(s)	526, 1138
AlwI	GGATC	9 cut(s)	76, 89, 623, 694, 1189, 2483, 2627, 2941, 2954
AlwNI	CAGNNNCTG	5 cut(s)	229, 1028, 1709, 2507, 2918
Ama87I	CYCGRG	2 cut(s)	57, 1532
AoxI	GGCC	4 cut(s)	385, 1613, 2883, 3006
ApaI	GGGCCC	1 cut(s)	2887
ApaLI	GTGCAC	2 cut(s)	526, 1138
ApoI	RAATTY	6 cut(s)	319, 473, 1859, 2159, 2402, 2862
AseI	ATTAAT	1 cut(s)	420
AsiGI	ACCGGT	1 cut(s)	866
AspLEI	GCGC	3 cut(s)	1714, 2759, 3130
AspS9I	GGNCC	4 cut(s)	869, 2387, 2883, 2884
AsuC2I	CCSGG	2 cut(s)	1533, 1534
AsuII	TTCGAA	1 cut(s)	562
AsuNHI	GCTAGC	3 cut(s)	38, 103, 1774
AvaI	CYCGRG	2 cut(s)	57, 1532
AvaII	GGWCC	2 cut(s)	869, 2387
AxyI	CCTNAGG	1 cut(s)	2391
BaeGI	GKGCMC	4 cut(s)	530, 1142, 1241, 2887
BalI	TGGCCA	1 cut(s)	387
BamHI	GGATCC	2 cut(s)	81, 2946
BanI	GGYRCC	6 cut(s)	23, 53, 1238, 1652, 2762, 2972
BanII	GRGCYC	3 cut(s)	417, 696, 2887
BarI	GAAGNNNNNNTAC	2 cut(s)	2971, 3003
BbsI	GAAGAC	2 cut(s)	135, 765
Bbv12I	GWGCWC	6 cut(s)	240, 417, 530, 696, 1142, 1762
BceAI	ACGGC	1 cut(s)	1449
BcgI	CGANNNNNNTGC	2 cut(s)	43, 77
BciT130I	CCWGG	3 cut(s)	1853, 2381, 2888
BciVI	GTATCC	1 cut(s)	2885
BclI	TGATCA	2 cut(s)	1596, 1780
BcnI	CCSGG	2 cut(s)	1533, 1534
BcoDI	GTCTC	5 cut(s)	971, 1700, 2621, 2862, 3035
BcuI	ACTAGT	2 cut(s)	1325, 1939
BfmI	CTRYAG	6 cut(s)	255, 1110, 1919, 1980, 2181, 2730
BfoI	RGCGCY	1 cut(s)	2760
BfrI	CTTAAG	2 cut(s)	278, 1520
BfuAI	ACCTGC	1 cut(s)	446
BfuI	GTATCC	1 cut(s)	2885
BglI	GCCNNNNNGGC	1 cut(s)	2981
BglII	AGATCT	3 cut(s)	875, 1785, 2836
BmcAI	AGTACT	2 cut(s)	1364, 1938
Bme1390I	CCNGG	5 cut(s)	1533, 1534, 1853, 2381, 2888
Bme18I	GGWCC	2 cut(s)	869, 2387
BmeT110I	CYCGRG	2 cut(s)	57, 1532
BmgBI	CACGTC	2 cut(s)	138, 823
BmgT120I	GGNCC	4 cut(s)	869, 2387, 2883, 2884
BmrFI	CCNGG	5 cut(s)	1533, 1534, 1853, 2381, 2888
BmrI	ACTGGG	4 cut(s)	458, 1244, 2010, 2952
BmtI	GCTAGC	3 cut(s)	42, 107, 1778
BmuI	ACTGGG	4 cut(s)	458, 1244, 2010, 2952
BoxI	GACNNNNGTC	1 cut(s)	1204
BpiI	GAAGAC	2 cut(s)	135, 765
BpmI	CTGGAG	1 cut(s)	2322
Bpu10I	CCTNAGC	2 cut(s)	506, 2280
Bpu14I	TTCGAA	1 cut(s)	562
BpuEI	CTTGAG	3 cut(s)	626, 995, 1027
BpuMI	CCSGG	2 cut(s)	1533, 1534
Bsa29I	ATCGAT	2 cut(s)	399, 2610
BsaI	GGTCTC	1 cut(s)	2621
BsaJI	CCNNGG	9 cut(s)	1284, 1532, 1533, 1852, 2637, 2886, 2887, 2902, 2960
BsaWI	WCCGGW	2 cut(s)	152, 866
BsaXI	ACNNNNNCTCC	2 cut(s)	2295, 2325
Bsc4I	CCNNNNNNNGG	6 cut(s)	352, 383, 1553, 1881, 2536, 2942
Bse118I	RCCGGY	3 cut(s)	501, 866, 2969
Bse21I	CCTNAGG	1 cut(s)	2391
Bse3DI	GCAATG	3 cut(s)	489, 1281, 1471
BseBI	CCWGG	3 cut(s)	1853, 2381, 2888
BseCI	ATCGAT	2 cut(s)	399, 2610
BseDI	CCNNGG	9 cut(s)	1284, 1532, 1533, 1852, 2637, 2886, 2887, 2902, 2960
BseLI	CCNNNNNNNGG	6 cut(s)	352, 383, 1553, 1881, 2536, 2942
BseMI	GCAATG	3 cut(s)	489, 1281, 1471
BseMII	CTCAG	8 cut(s)	520, 531, 681, 995, 1488, 1947, 2382, 2616
BseSI	GKGCMC	4 cut(s)	530, 1142, 1241, 2887
BseYI	CCCAGC	2 cut(s)	1837, 2468
BsgI	GTGCAG	2 cut(s)	1169, 1728
BshFI	GGCC	4 cut(s)	387, 1615, 2885, 3008
BshNI	GGYRCC	6 cut(s)	23, 53, 1238, 1652, 2762, 2972
BshTI	ACCGGT	1 cut(s)	866
BshVI	ATCGAT	2 cut(s)	399, 2610
BsiHKAI	GWGCWC	6 cut(s)	240, 417, 530, 696, 1142, 1762
BsiHKCI	CYCGRG	2 cut(s)	57, 1532
BsiSI	CCGG	8 cut(s)	114, 153, 502, 867, 1533, 2970, 3101, 3165
BslFI	GGGAC	2 cut(s)	1088, 2107
BslI	CCNNNNNNNGG	6 cut(s)	352, 383, 1553, 1881, 2536, 2942
BsmAI	GTCTC	5 cut(s)	971, 1700, 2621, 2862, 3035
BsmFI	GGGAC	2 cut(s)	1088, 2107
BsmI	GAATGC	2 cut(s)	667, 1122
BsnI	GGCC	4 cut(s)	387, 1615, 2885, 3008
Bso31I	GGTCTC	1 cut(s)	2621
BsoBI	CYCGRG	2 cut(s)	57, 1532
Bsp119I	TTCGAA	1 cut(s)	562
Bsp120I	GGGCCC	1 cut(s)	2883
Bsp1286I	GDGCHC	8 cut(s)	240, 417, 530, 696, 1142, 1241, 1762, 2887
Bsp1407I	TGTACA	2 cut(s)	987, 1086
Bsp19I	CCATGG	1 cut(s)	2637
BspACI	CCGC	4 cut(s)	21, 189, 2365, 2921
BspANI	GGCC	4 cut(s)	387, 1615, 2885, 3008
BspCNI	CTCAG	8 cut(s)	519, 532, 682, 994, 1487, 1946, 2383, 2617
BspDI	ATCGAT	2 cut(s)	399, 2610
BspHI	TCATGA	1 cut(s)	657
BspMAI	CTGCAG	4 cut(s)	259, 1923, 2185, 2734
BspMI	ACCTGC	1 cut(s)	446
BspOI	GCTAGC	3 cut(s)	42, 107, 1778
BspPI	GGATC	9 cut(s)	76, 89, 623, 694, 1189, 2483, 2627, 2941, 2954
BspQI	GCTCTTC	2 cut(s)	564, 2798
BspT104I	TTCGAA	1 cut(s)	562
BspT107I	GGYRCC	6 cut(s)	23, 53, 1238, 1652, 2762, 2972
BspTI	CTTAAG	2 cut(s)	278, 1520
BspTNI	GGTCTC	1 cut(s)	2621
BsrDI	GCAATG	3 cut(s)	489, 1281, 1471
BsrFI	RCCGGY	3 cut(s)	501, 866, 2969
BsrGI	TGTACA	2 cut(s)	987, 1086
BssAI	RCCGGY	3 cut(s)	501, 866, 2969
BssECI	CCNNGG	9 cut(s)	1284, 1532, 1533, 1852, 2637, 2886, 2887, 2902, 2960
BssT1I	CCWWGG	4 cut(s)	1284, 2637, 2902, 2960
Bst2UI	CCWGG	3 cut(s)	1853, 2381, 2888
Bst6I	CTCTTC	5 cut(s)	564, 949, 2314, 2798, 3021
BstAFI	CTTAAG	2 cut(s)	278, 1520
BstAPI	GCANNNNNTGC	2 cut(s)	534, 2591
BstAUI	TGTACA	2 cut(s)	987, 1086
BstBI	TTCGAA	1 cut(s)	562
BstDSI	CCRYGG	1 cut(s)	2637
BstH2I	RGCGCY	1 cut(s)	2760
BstHHI	GCGC	3 cut(s)	1714, 2759, 3130
BstMAI	GTCTC	5 cut(s)	971, 1700, 2621, 2862, 3035
BstNI	CCWGG	3 cut(s)	1853, 2381, 2888
BstNSI	RCATGY	8 cut(s)	1105, 1953, 2231, 2270, 2439, 2514, 2911, 3062
BstPAI	GACNNNNGTC	1 cut(s)	1204
BstSCI	CCNGG	5 cut(s)	1531, 1532, 1851, 2379, 2886
BstSFI	CTRYAG	6 cut(s)	255, 1110, 1919, 1980, 2181, 2730
BstSLI	GKGCMC	4 cut(s)	530, 1142, 1241, 2887
BstV2I	GAAGAC	2 cut(s)	135, 765
BstX2I	RGATCY	7 cut(s)	81, 686, 875, 1785, 2488, 2836, 2946
BstXI	CCANNNNNNTGG	2 cut(s)	34, 185
BstYI	RGATCY	7 cut(s)	81, 686, 875, 1785, 2488, 2836, 2946
Bsu15I	ATCGAT	2 cut(s)	399, 2610
Bsu36I	CCTNAGG	1 cut(s)	2391
BsuI	GTATCC	1 cut(s)	2885
BsuRI	GGCC	4 cut(s)	387, 1615, 2885, 3008
BsuTUI	ATCGAT	2 cut(s)	399, 2610
BtgI	CCRYGG	1 cut(s)	2637
BtgZI	GCGATG	1 cut(s)	57
BtrI	CACGTC	2 cut(s)	138, 823
BtsI	GCAGTG	1 cut(s)	1916
BveI	ACCTGC	1 cut(s)	446
CaiI	CAGNNNCTG	5 cut(s)	229, 1028, 1709, 2507, 2918
CciI	TCATGA	1 cut(s)	657
CfoI	GCGC	3 cut(s)	1714, 2759, 3130
Cfr10I	RCCGGY	3 cut(s)	501, 866, 2969
Cfr13I	GGNCC	4 cut(s)	869, 2387, 2883, 2884
Cfr9I	CCCGGG	1 cut(s)	1532
ClaI	ATCGAT	2 cut(s)	399, 2610
Csp6I	GTAC	6 cut(s)	988, 1087, 1363, 1937, 2166, 2294
CspAI	ACCGGT	1 cut(s)	866
CviQI	GTAC	6 cut(s)	988, 1087, 1363, 1937, 2166, 2294
DraIII	CACNNNGTG	1 cut(s)	2021
EaeI	YGGCCR	1 cut(s)	385
Eam1104I	CTCTTC	5 cut(s)	564, 949, 2314, 2798, 3021
EarI	CTCTTC	5 cut(s)	564, 949, 2314, 2798, 3021
EciI	GGCGGA	1 cut(s)	204
Ecl136II	GAGCTC	2 cut(s)	415, 694
Eco130I	CCWWGG	4 cut(s)	1284, 2637, 2902, 2960
Eco147I	AGGCCT	1 cut(s)	1615
Eco24I	GRGCYC	3 cut(s)	417, 696, 2887
Eco31I	GGTCTC	1 cut(s)	2621
Eco32I	GATATC	2 cut(s)	217, 1341
Eco47I	GGWCC	2 cut(s)	869, 2387
Eco53kI	GAGCTC	2 cut(s)	415, 694
Eco57I	CTGAAG	1 cut(s)	777
Eco81I	CCTNAGG	1 cut(s)	2391
Eco88I	CYCGRG	2 cut(s)	57, 1532
EcoICRI	GAGCTC	2 cut(s)	415, 694
EcoO109I	RGGNCCY	1 cut(s)	2884
EcoRI	GAATTC	1 cut(s)	2159
EcoRII	CCWGG	3 cut(s)	1851, 2379, 2886
EcoRV	GATATC	2 cut(s)	217, 1341
EcoT14I	CCWWGG	4 cut(s)	1284, 2637, 2902, 2960
EcoT38I	GRGCYC	3 cut(s)	417, 696, 2887
ErhI	CCWWGG	4 cut(s)	1284, 2637, 2902, 2960
FalI	AAGNNNNNCTT	4 cut(s)	608, 640, 2010, 2042
FaqI	GGGAC	2 cut(s)	1088, 2107
FauI	CCCGC	2 cut(s)	2358, 2914
FbaI	TGATCA	2 cut(s)	1596, 1780
FriOI	GRGCYC	3 cut(s)	417, 696, 2887
GlaI	GCGC	3 cut(s)	1713, 2758, 3129
GsaI	CCCAGC	2 cut(s)	1841, 2472
GsuI	CTGGAG	1 cut(s)	2322
HaeII	RGCGCY	1 cut(s)	2760
HaeIII	GGCC	4 cut(s)	387, 1615, 2885, 3008
HapII	CCGG	8 cut(s)	114, 153, 502, 867, 1533, 2970, 3101, 3165
HhaI	GCGC	3 cut(s)	1714, 2759, 3130
Hin6I	GCGC	3 cut(s)	1712, 2757, 3128
HinP1I	GCGC	3 cut(s)	1712, 2757, 3128
HincII	GTYRAC	2 cut(s)	2259, 3078
HindII	GTYRAC	2 cut(s)	2259, 3078
HindIII	AAGCTT	1 cut(s)	931
HpaII	CCGG	8 cut(s)	114, 153, 502, 867, 1533, 2970, 3101, 3165
Hpy99I	CGWCG	1 cut(s)	142
HpyAV	CCTTC	2 cut(s)	734, 1999
HpyCH4IV	ACGT	3 cut(s)	137, 822, 1201
HpySE526I	ACGT	3 cut(s)	137, 822, 1201
HspAI	GCGC	3 cut(s)	1712, 2757, 3128
KroI	GCCGGC	1 cut(s)	501
KroNI	GCCGGC	1 cut(s)	503
Ksp22I	TGATCA	2 cut(s)	1596, 1780
LguI	GCTCTTC	2 cut(s)	564, 2798
LmnI	GCTCC	3 cut(s)	235, 2224, 2303
MaeII	ACGT	3 cut(s)	137, 822, 1201
MfeI	CAATTG	2 cut(s)	2589, 2751
MflI	RGATCY	7 cut(s)	81, 686, 875, 1785, 2488, 2836, 2946
MhlI	GDGCHC	8 cut(s)	240, 417, 530, 696, 1142, 1241, 1762, 2887
MlsI	TGGCCA	1 cut(s)	387
MluNI	TGGCCA	1 cut(s)	387
MlyI	GAGTC	7 cut(s)	69, 551, 973, 2708, 3037, 3091, 3155
MmeI	TCCRAC	3 cut(s)	213, 1829, 2086
Mox20I	TGGCCA	1 cut(s)	387
MroNI	GCCGGC	1 cut(s)	501
MscI	TGGCCA	1 cut(s)	387
MslI	CAYNNNNRTG	3 cut(s)	183, 243, 1145
Msp20I	TGGCCA	1 cut(s)	387
MspCI	CTTAAG	2 cut(s)	278, 1520
MspI	CCGG	8 cut(s)	114, 153, 502, 867, 1533, 2970, 3101, 3165
MspR9I	CCNGG	5 cut(s)	1533, 1534, 1853, 2381, 2888
MunI	CAATTG	2 cut(s)	2589, 2751
Mva1269I	GAATGC	2 cut(s)	667, 1122
MvaI	CCWGG	3 cut(s)	1853, 2381, 2888
NaeI	GCCGGC	1 cut(s)	503
NciI	CCSGG	2 cut(s)	1533, 1534
NcoI	CCATGG	1 cut(s)	2637
NgoMIV	GCCGGC	1 cut(s)	501
NheI	GCTAGC	3 cut(s)	38, 103, 1774
NmuCI	GTSAC	8 cut(s)	121, 127, 133, 372, 838, 1634, 2327, 2686
NspI	RCATGY	8 cut(s)	1105, 1953, 2231, 2270, 2439, 2514, 2911, 3062
NspV	TTCGAA	1 cut(s)	562
OliI	CACNNNNGTG	1 cut(s)	1145
PaeI	GCATGC	4 cut(s)	1953, 2231, 2911, 3062
PagI	TCATGA	1 cut(s)	657
PasI	CCCWGGG	1 cut(s)	2887
PceI	AGGCCT	1 cut(s)	1615
PciI	ACATGT	2 cut(s)	2266, 2435
PciSI	GCTCTTC	2 cut(s)	564, 2798
PcsI	WCGNNNNNNNCGW	1 cut(s)	3027
PctI	GAATGC	2 cut(s)	667, 1122
PdiI	GCCGGC	1 cut(s)	503
PfeI	GAWTC	9 cut(s)	170, 329, 551, 788, 1093, 1132, 2655, 2706, 2925
PflFI	GACNNNGTC	1 cut(s)	2854
PflMI	CCANNNNNTGG	2 cut(s)	1881, 2536
PinAI	ACCGGT	1 cut(s)	866
PleI	GAGTC	7 cut(s)	68, 550, 973, 2708, 3037, 3091, 3155
PpsI	GAGTC	7 cut(s)	68, 550, 973, 2708, 3037, 3091, 3155
PscI	ACATGT	2 cut(s)	2266, 2435
PshAI	GACNNNNGTC	1 cut(s)	1204
PshBI	ATTAAT	1 cut(s)	420
Psp124BI	GAGCTC	2 cut(s)	417, 696
Psp6I	CCWGG	3 cut(s)	1851, 2379, 2886
PspFI	CCCAGC	2 cut(s)	1837, 2468
PspGI	CCWGG	3 cut(s)	1851, 2379, 2886
PspOMI	GGGCCC	1 cut(s)	2883
PspPI	GGNCC	4 cut(s)	869, 2387, 2883, 2884
PstI	CTGCAG	4 cut(s)	259, 1923, 2185, 2734
PstNI	CAGNNNCTG	5 cut(s)	229, 1028, 1709, 2507, 2918
PsuI	RGATCY	7 cut(s)	81, 686, 875, 1785, 2488, 2836, 2946
PsyI	GACNNNGTC	1 cut(s)	2854
RsaI	GTAC	6 cut(s)	989, 1088, 1364, 1938, 2167, 2295
RsaNI	GTAC	6 cut(s)	988, 1087, 1363, 1937, 2166, 2294
RseI	CAYNNNNRTG	3 cut(s)	183, 243, 1145
SacI	GAGCTC	2 cut(s)	417, 696
SapI	GCTCTTC	2 cut(s)	564, 2798
Sau96I	GGNCC	4 cut(s)	869, 2387, 2883, 2884
ScaI	AGTACT	2 cut(s)	1364, 1938
SchI	GAGTC	7 cut(s)	69, 551, 973, 2708, 3037, 3091, 3155
ScrFI	CCNGG	5 cut(s)	1533, 1534, 1853, 2381, 2888
SduI	GDGCHC	8 cut(s)	240, 417, 530, 696, 1142, 1241, 1762, 2887
SfcI	CTRYAG	6 cut(s)	255, 1110, 1919, 1980, 2181, 2730
SfuI	TTCGAA	1 cut(s)	562
SinI	GGWCC	2 cut(s)	869, 2387
SmaI	CCCGGG	1 cut(s)	1534
SmiMI	CAYNNNNRTG	3 cut(s)	183, 243, 1145
SmlI	CTYRAG	5 cut(s)	278, 605, 974, 1006, 1520
SmoI	CTYRAG	5 cut(s)	278, 605, 974, 1006, 1520
SpeI	ACTAGT	2 cut(s)	1325, 1939
SphI	GCATGC	4 cut(s)	1953, 2231, 2911, 3062
SseBI	AGGCCT	1 cut(s)	1615
SsiI	CCGC	4 cut(s)	21, 189, 2365, 2921
SstI	GAGCTC	2 cut(s)	417, 696
StuI	AGGCCT	1 cut(s)	1615
StyD4I	CCNGG	5 cut(s)	1531, 1532, 1851, 2379, 2886
StyI	CCWWGG	4 cut(s)	1284, 2637, 2902, 2960
TaiI	ACGT	3 cut(s)	140, 825, 1204
TaqI	TCGA	9 cut(s)	63, 399, 562, 653, 2431, 2610, 2860, 3095, 3159
TaqII	GACCGA	1 cut(s)	2645
TatI	WGTACW	5 cut(s)	987, 1086, 1362, 1936, 2165
TauI	GCSGC	1 cut(s)	24
TfiI	GAWTC	9 cut(s)	170, 329, 551, 788, 1093, 1132, 2655, 2706, 2925
TseFI	GTSAC	8 cut(s)	121, 127, 133, 372, 838, 1634, 2327, 2686
Tsp45I	GTSAC	8 cut(s)	121, 127, 133, 372, 838, 1634, 2327, 2686
TspGWI	ACGGA	1 cut(s)	94
TspMI	CCCGGG	1 cut(s)	1532
Tth111I	GACNNNGTC	1 cut(s)	2854
Van91I	CCANNNNNTGG	2 cut(s)	1881, 2536
Vha464I	CTTAAG	2 cut(s)	278, 1520
VneI	GTGCAC	2 cut(s)	526, 1138
VpaK11BI	GGWCC	2 cut(s)	869, 2387
VspI	ATTAAT	1 cut(s)	420
XapI	RAATTY	6 cut(s)	319, 473, 1859, 2159, 2402, 2862
XbaI	TCTAGA	1 cut(s)	2843
XceI	RCATGY	8 cut(s)	1105, 1953, 2231, 2270, 2439, 2514, 2911, 3062
XcmI	CCANNNNNNNNNTGG	2 cut(s)	331, 2149
XmaI	CCCGGG	1 cut(s)	1532
ZrmI	AGTACT	2 cut(s)	1364, 1938

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.