Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0005184

Physical Location & Seq

Genomic Coordinates Forward (+)

51137749 .. 51146426

Gene Structure 8678 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG388200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2706 bp

ATGGATTCAAACCATTTGAACGGCGGAGTGGATGCTATCAAACTGCCTCTGTTGGAGGAGCATAAGAGAATTGAACACTTAAGTTCAACAGGTTTCCCAGTTGATGAATTTCCAGAACAAGACATTGCAGTATGCCGGCTCAGGATAAAAGGAATGATGTGCACCAGTTGCTCCGAGTCCGTTGAATCTGCTCTTCGAATGGTTGATGGAGTGAAAAACGCAGTTGTTGGTCTAGCCCTTGAGGAAGCAAAGGTTCACTTTGATCCAAACATCACTGACACCTGTTCGATCATGAAAGCAATAGAAGTTGCTGGCTTTGGATCTGAGCTCATTAGCTCAGGGAATGATGTAAACAAAGTGCATCTAAAACTTGAAGGAGTTAATTCTCCTGAAGACATGACCATCATCCAATCCTCCCTTGAATCAGTTGAGGGTGTAAATAATGTTGAAGTGGACGTGTTAGAAAAAAAGGTCACTATTACCTACGATGCAGACCTCACTGGTCCAAGATCTCTTATACATTGTATTGAAGAAGCTGGAAGTAACCCCAAATCATACCAAGCAAGCTTGTATGTTCCTCCAAGACGAAGAGAAGCTGAACAGCAGCTTGAGACTCAGATGTACAGGAACCAGTTTTTCTTGAGCTGTCTATTTTCAGTTCCTGTATTTTTATTCTCAATGGTGCTTCCAATGCTTTCTCCCTATGGGGACTGGTTAATGTACAAGATTCACAACATGCTCACTGTAGGAATGCTTTTAAGATGGATTCTGTGCACACCCGTGCAGTTCATTATTGGCAGGAGGTTTTATGTGGGATCATATCATGCTTTAAGACGTAGGTCAGCTAATATGGATGTTCTTGTTGCACTGGGCACCAATGTTGCTTATTTCTACTCTGTCTATATAGCAATGAAAGCATTGGCTTTGGATAATTTTGAGGGGGAAGATTTCTTTGAAACTAGTTCCATGTTGATATCCTTTATACTCTTGGGGAAGTACTTAGAAGTTCTAGCTAGAGGAAAAACATCTGATGCTTTAGCGAAGCTGACAGACCTTACTCCTGAAACGGCTTATCTGCTATCACTAGATGCTGATGGCAATGCTATCTCAGAGATGGAAATTAGCACTCAACTTATTCAAAGAAATGATATACTTAAGATTGTTCCCGGGGCAAAAGTTCCTGTTGATGGGATTGTAATTAGTGGTCAAAGCCATGTGAACGAGAGTATGATCACAGGAGAGGCAAGGCCTATTTCTAAAAGATTAGGTGACAAGGTAATTGGTGGCACCATGAATGAGAATGGATGCTTACAGGTTAAGGCAACCCATGTTGGTTCAGAGACTGCGCTATCCCAGATAGTTCAACTTGTTGAAGCTGCACAGCTTTCCAGAGCACCTGTTCAGAAGCTAGCTGATCAGATCTCAAAGTTTTTTGTTCCAACAGTTGTGATTGCAGCATTTCTGACATGGCTGGGTTGGTTCATCCTGGGAGAATTCGGTCTTTATCCCATATCATGGACACCAAAAGGCATGGATAGGTTTGAGCTGGCACTGCAGTTCGGCATCTCAGTACTAGTGGTTGCATGCCCTTGTGCTTTGGGATTAGCAACACCTACAGCAGTCATGGTTGCAACAGGGAAGGGTGCTACACAAGGTGTTCTTATCAAGGGTGGCAATGCACTTGAAAAGGCTCATAAGGTGAAAACAGTTGTCTTTGATAAGACAGGGACACTGACAGTTGGAAAACCGACAGTAGTTAGTGCTGTGCTATTTTCCAATTACTCAATGGAGGAATTCTGTACTGTGGCTACTGCTGCAGAGGTAAATAGTGAACACCCAATAGCAAAGTCTATTGTGGATCATGCAAAGAGGTTTCTTAACAAGTTTGGGTCAACTGAACATGTGGTGGAGGCTAAGGACTTTGAGGTACACACTGGAGCTGGTGTTAGTGGAAGAGTTGGTGACAAAATGGTTTTGGTTGGGAACAAGAGGCTCATGCGGGAATATAATGTCCAGGTTGGTCCTGAGGTTGAGAAATTCGTTTCAGAAAATGAGAAACTGGCTCGAACATGTGTGTTAGTTTCCATTGATGGAAAGGTTGCTGGGTCGTTTGCTGTAACTGATCCAGTGAAGCCAGAGGCTGCATGTGTAATATCTTATCTCCACTCAATGGGCATTTCAAGCATCATGGTTACTGGTGATAATCGGGCAACAGCAGCAGCAATTGCAAGGGAGGTTGGCATCGATGAGGTGTTTGCTGAGACCGATCCCATGGGAAAAGCTGATAGAATCAAAGAGTTACAGATGAAAGGATTGACTGTGACTATGGTGGGAGATGGAATCAATGACTCACCAGCTTTGGCTGCAGCAGATGTTGGCATGGCAATTGGCGCTGGCACCGATGTAGCAATAGAAGCTGCTGATATAGTTTTGATGAAAAGCAATTTGGAAGATGTAGTTACAGCCATAGATCTCTCTAGAAAGACTATGTCTCGAATTTGGTTGAATTATGTCTGGGCCCTGGGATACAACATCCTTGGCATGCCAGTTGCTGCCGGAATCTTGTTCCCTTTCACTGGGATCCGATTACCACCTTGGCTTGCCGGTGCCTGTATGGCTGCTTCATCTGTTAGTGTGGTATGTTCATCTCTCTTGTTGCAGTCTTATAAGAAGCCTTTGCATTTTCAGAACATTAAGTCTGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

901

Amino Acids

97.57

Weight (kDa)

5.59

Isoelectric Point (pI)

37.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	47 - 106	8.5e-08	Heavy-metal-associated domain
HMA	PF00403	136 - 180	4.4e-07	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	354 - 458	7e-25	P-type ATPase actuator domain
Hydrolase	PF00702	567 - 791	5.3e-34	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005184)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190 FvH4_3g29190
malus_domestica	MD11G1199600.v1.1
prunus_persica	Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1 Prupe.4G243400_v2.0.a1
pyrus_communis	pycom11g17250
rosa_chinensis	RchiOBHm_Chr5g0053501 RchiOBHm_Chr5g0053621
rosa_laevigata	RLG00000034888 RLG00000034889 RLG00000034890
rosa_multiflora	Rmu_co8389717.1_g000001 Rmu_sc0003228.1_g000003 Rmu_sc0005966.1_g000022
rosa_roxburghii	Rroxscaffold_1G00026920
rosa_rugosa	Rorug05G0280400 Rorug05G0280500
rosa_samantha	Rh5AG351600 Rh5AG352300 Rh5BG363900 Rh5CG388200 Rh5DG378500
rosa_wichuraiana	Rw5G033260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2667
AccB1I	GGYRCC	4 cut(s)	872, 1286, 2396, 2606
AccB7I	CCANNNNNTGG	2 cut(s)	1515, 2170
AciI	CCGC	2 cut(s)	24, 1999
AclWI	GGATC	8 cut(s)	257, 328, 823, 1866, 2117, 2261, 2575, 2588
AcsI	RAATTY	5 cut(s)	107, 1493, 1793, 2036, 2496
AcuI	CTGAAG	1 cut(s)	411
AdeI	CACNNNGTG	1 cut(s)	1655
AfaI	GTAC	6 cut(s)	623, 722, 998, 1572, 1801, 1929
AfiI	CCNNNNNNNGG	4 cut(s)	1187, 1515, 2170, 2576
AflII	CTTAAG	2 cut(s)	79, 1154
AflIII	ACRYGT	3 cut(s)	456, 1900, 2069
AhlI	ACTAGT	2 cut(s)	959, 1573
AjiI	CACGTC	1 cut(s)	457
AjnI	CCWGG	3 cut(s)	1485, 2013, 2520
AjuI	GAANNNNNNNTTGG	2 cut(s)	2429, 2461
AleI	CACNNNNGTG	1 cut(s)	779
Alw21I	GWGCWC	4 cut(s)	164, 330, 776, 1396
Alw26I	GTCTC	4 cut(s)	605, 1334, 2255, 2496
Alw44I	GTGCAC	2 cut(s)	160, 772
AlwI	GGATC	8 cut(s)	257, 328, 823, 1866, 2117, 2261, 2575, 2588
AlwNI	CAGNNNCTG	4 cut(s)	662, 1343, 2141, 2552
Ama87I	CYCGRG	1 cut(s)	1166
AoxI	GGCC	2 cut(s)	1247, 2517
ApaI	GGGCCC	1 cut(s)	2521
ApaLI	GTGCAC	2 cut(s)	160, 772
ApoI	RAATTY	5 cut(s)	107, 1493, 1793, 2036, 2496
AspLEI	GCGC	2 cut(s)	1348, 2393
AspS9I	GGNCC	4 cut(s)	503, 2021, 2517, 2518
AsuC2I	CCSGG	2 cut(s)	1167, 1168
AsuHPI	GGTGA	5 cut(s)	1280, 1711, 1973, 2210, 2343
AsuII	TTCGAA	1 cut(s)	196
AsuNHI	GCTAGC	1 cut(s)	1408
AvaI	CYCGRG	1 cut(s)	1166
AvaII	GGWCC	2 cut(s)	503, 2021
AxyI	CCTNAGG	1 cut(s)	2025
BaeGI	GKGCMC	4 cut(s)	164, 776, 875, 2521
BamHI	GGATCC	1 cut(s)	2580
BanI	GGYRCC	4 cut(s)	872, 1286, 2396, 2606
BanII	GRGCYC	2 cut(s)	330, 2521
BarI	GAAGNNNNNNTAC	2 cut(s)	2605, 2637
BbsI	GAAGAC	1 cut(s)	399
Bbv12I	GWGCWC	4 cut(s)	164, 330, 776, 1396
BccI	CCATC	8 cut(s)	200, 410, 756, 1088, 1108, 1181, 2084, 2330
BceAI	ACGGC	2 cut(s)	37, 1083
BciT130I	CCWGG	3 cut(s)	1487, 2015, 2522
BciVI	GTATCC	1 cut(s)	2519
BclI	TGATCA	2 cut(s)	1230, 1414
BcnI	CCSGG	2 cut(s)	1167, 1168
BcoDI	GTCTC	4 cut(s)	605, 1334, 2255, 2496
BcuI	ACTAGT	2 cut(s)	959, 1573
BfaI	CTAG	8 cut(s)	233, 960, 1010, 1014, 1085, 1409, 1574, 2478
BfmI	CTRYAG	5 cut(s)	744, 1553, 1614, 1815, 2364
BfoI	RGCGCY	1 cut(s)	2394
BfrI	CTTAAG	2 cut(s)	79, 1154
BfuI	GTATCC	1 cut(s)	2519
BglI	GCCNNNNNGGC	1 cut(s)	2615
BglII	AGATCT	3 cut(s)	509, 1419, 2470
BmcAI	AGTACT	2 cut(s)	998, 1572
Bme1390I	CCNGG	5 cut(s)	1167, 1168, 1487, 2015, 2522
Bme18I	GGWCC	2 cut(s)	503, 2021
BmeT110I	CYCGRG	1 cut(s)	1166
BmgBI	CACGTC	1 cut(s)	457
BmgT120I	GGNCC	4 cut(s)	503, 2021, 2517, 2518
BmiI	GGNNCC	7 cut(s)	629, 874, 1288, 2398, 2519, 2582, 2608
BmrFI	CCNGG	5 cut(s)	1167, 1168, 1487, 2015, 2522
BmrI	ACTGGG	3 cut(s)	92, 878, 2586
BmsI	GCATC	9 cut(s)	22, 370, 478, 1021, 1078, 1295, 1572, 2193, 2250
BmtI	GCTAGC	1 cut(s)	1412
BmuI	ACTGGG	3 cut(s)	92, 878, 2586
BoxI	GACNNNNGTC	1 cut(s)	838
BpiI	GAAGAC	1 cut(s)	399
BpmI	CTGGAG	1 cut(s)	1956
Bpu10I	CCTNAGC	3 cut(s)	140, 337, 1914
Bpu14I	TTCGAA	1 cut(s)	196
BpuEI	CTTGAG	3 cut(s)	260, 629, 661
BpuMI	CCSGG	2 cut(s)	1167, 1168
Bsa29I	ATCGAT	1 cut(s)	2244
BsaI	GGTCTC	1 cut(s)	2255
BsaJI	CCNNGG	8 cut(s)	1166, 1167, 1486, 2271, 2520, 2521, 2536, 2594
BsaXI	ACNNNNNCTCC	2 cut(s)	1929, 1959
Bsc4I	CCNNNNNNNGG	4 cut(s)	1187, 1515, 2170, 2576
Bse118I	RCCGGY	2 cut(s)	135, 2603
Bse21I	CCTNAGG	1 cut(s)	2025
Bse3DI	GCAATG	4 cut(s)	123, 915, 1105, 1681
BseBI	CCWGG	3 cut(s)	1487, 2015, 2522
BseCI	ATCGAT	1 cut(s)	2244
BseDI	CCNNGG	8 cut(s)	1166, 1167, 1486, 2271, 2520, 2521, 2536, 2594
BseGI	GGATG	6 cut(s)	37, 405, 859, 1310, 1482, 2532
BseLI	CCNNNNNNNGG	4 cut(s)	1187, 1515, 2170, 2576
BseMI	GCAATG	4 cut(s)	123, 915, 1105, 1681
BseMII	CTCAG	8 cut(s)	154, 315, 351, 629, 1122, 1581, 2016, 2250
BseRI	GAGGAG	1 cut(s)	71
BseSI	GKGCMC	4 cut(s)	164, 776, 875, 2521
BseYI	CCCAGC	2 cut(s)	1471, 2102
BsgI	GTGCAG	2 cut(s)	803, 1362
BshFI	GGCC	2 cut(s)	1249, 2519
BshNI	GGYRCC	4 cut(s)	872, 1286, 2396, 2606
BshVI	ATCGAT	1 cut(s)	2244
BsiHKAI	GWGCWC	4 cut(s)	164, 330, 776, 1396
BsiHKCI	CYCGRG	1 cut(s)	1166
BsiSI	CCGG	4 cut(s)	136, 1167, 2556, 2604
BslFI	GGGAC	2 cut(s)	722, 1741
BslI	CCNNNNNNNGG	4 cut(s)	1187, 1515, 2170, 2576
BsmAI	GTCTC	4 cut(s)	605, 1334, 2255, 2496
BsmFI	GGGAC	2 cut(s)	722, 1741
BsmI	GAATGC	1 cut(s)	756
BsnI	GGCC	2 cut(s)	1249, 2519
Bso31I	GGTCTC	1 cut(s)	2255
BsoBI	CYCGRG	1 cut(s)	1166
Bsp119I	TTCGAA	1 cut(s)	196
Bsp120I	GGGCCC	1 cut(s)	2517
Bsp1286I	GDGCHC	6 cut(s)	164, 330, 776, 875, 1396, 2521
Bsp1407I	TGTACA	2 cut(s)	621, 720
Bsp19I	CCATGG	1 cut(s)	2271
BspACI	CCGC	2 cut(s)	24, 1999
BspANI	GGCC	2 cut(s)	1249, 2519
BspCNI	CTCAG	8 cut(s)	153, 316, 350, 628, 1121, 1580, 2017, 2251
BspDI	ATCGAT	1 cut(s)	2244
BspHI	TCATGA	1 cut(s)	291
BspLI	GGNNCC	7 cut(s)	629, 874, 1288, 2398, 2519, 2582, 2608
BspMAI	CTGCAG	3 cut(s)	1557, 1819, 2368
BspOI	GCTAGC	1 cut(s)	1412
BspPI	GGATC	8 cut(s)	257, 328, 823, 1866, 2117, 2261, 2575, 2588
BspQI	GCTCTTC	1 cut(s)	198
BspT104I	TTCGAA	1 cut(s)	196
BspT107I	GGYRCC	4 cut(s)	872, 1286, 2396, 2606
BspTI	CTTAAG	2 cut(s)	79, 1154
BspTNI	GGTCTC	1 cut(s)	2255
BsrDI	GCAATG	4 cut(s)	123, 915, 1105, 1681
BsrFI	RCCGGY	2 cut(s)	135, 2603
BsrGI	TGTACA	2 cut(s)	621, 720
BssAI	RCCGGY	2 cut(s)	135, 2603
BssECI	CCNNGG	8 cut(s)	1166, 1167, 1486, 2271, 2520, 2521, 2536, 2594
BssT1I	CCWWGG	3 cut(s)	2271, 2536, 2594
Bst2UI	CCWGG	3 cut(s)	1487, 2015, 2522
Bst4CI	ACNGT	7 cut(s)	745, 1444, 1708, 1738, 1753, 1804, 2320
Bst6I	CTCTTC	3 cut(s)	198, 583, 1948
BstAFI	CTTAAG	2 cut(s)	79, 1154
BstAPI	GCANNNNNTGC	2 cut(s)	168, 2225
BstAUI	TGTACA	2 cut(s)	621, 720
BstBI	TTCGAA	1 cut(s)	196
BstC8I	GCNNGC	9 cut(s)	137, 313, 565, 1410, 1548, 1585, 2395, 2543, 2601
BstDSI	CCRYGG	1 cut(s)	2271
BstF5I	GGATG	6 cut(s)	37, 405, 859, 1310, 1482, 2532
BstH2I	RGCGCY	1 cut(s)	2394
BstHHI	GCGC	2 cut(s)	1348, 2393
BstMAI	GTCTC	4 cut(s)	605, 1334, 2255, 2496
BstNI	CCWGG	3 cut(s)	1487, 2015, 2522
BstNSI	RCATGY	6 cut(s)	739, 1587, 1904, 2073, 2148, 2545
BstPAI	GACNNNNGTC	1 cut(s)	838
BstSCI	CCNGG	5 cut(s)	1165, 1166, 1485, 2013, 2520
BstSFI	CTRYAG	5 cut(s)	744, 1553, 1614, 1815, 2364
BstSLI	GKGCMC	4 cut(s)	164, 776, 875, 2521
BstV2I	GAAGAC	1 cut(s)	399
BstX2I	RGATCY	5 cut(s)	320, 509, 1419, 2470, 2580
BstYI	RGATCY	5 cut(s)	320, 509, 1419, 2470, 2580
Bsu15I	ATCGAT	1 cut(s)	2244
Bsu36I	CCTNAGG	1 cut(s)	2025
BsuI	GTATCC	1 cut(s)	2519
BsuRI	GGCC	2 cut(s)	1249, 2519
BsuTUI	ATCGAT	1 cut(s)	2244
BtgI	CCRYGG	1 cut(s)	2271
BtrI	CACGTC	1 cut(s)	457
BtsCI	GGATG	6 cut(s)	37, 405, 859, 1310, 1482, 2532
BtsI	GCAGTG	1 cut(s)	1550
BtsIMutI	CAGTG	9 cut(s)	273, 498, 741, 866, 1550, 1730, 1932, 2133, 2574
Cac8I	GCNNGC	9 cut(s)	137, 313, 565, 1410, 1548, 1585, 2395, 2543, 2601
CaiI	CAGNNNCTG	4 cut(s)	662, 1343, 2141, 2552
CciI	TCATGA	1 cut(s)	291
CfoI	GCGC	2 cut(s)	1348, 2393
Cfr10I	RCCGGY	2 cut(s)	135, 2603
Cfr13I	GGNCC	4 cut(s)	503, 2021, 2517, 2518
Cfr9I	CCCGGG	1 cut(s)	1166
ClaI	ATCGAT	1 cut(s)	2244
Csp6I	GTAC	6 cut(s)	622, 721, 997, 1571, 1800, 1928
CviQI	GTAC	6 cut(s)	622, 721, 997, 1571, 1800, 1928
DraIII	CACNNNGTG	1 cut(s)	1655
Eam1104I	CTCTTC	3 cut(s)	198, 583, 1948
EarI	CTCTTC	3 cut(s)	198, 583, 1948
EciI	GGCGGA	1 cut(s)	39
Ecl136II	GAGCTC	1 cut(s)	328
Eco130I	CCWWGG	3 cut(s)	2271, 2536, 2594
Eco147I	AGGCCT	1 cut(s)	1249
Eco24I	GRGCYC	2 cut(s)	330, 2521
Eco31I	GGTCTC	1 cut(s)	2255
Eco32I	GATATC	1 cut(s)	975
Eco47I	GGWCC	2 cut(s)	503, 2021
Eco53kI	GAGCTC	1 cut(s)	328
Eco57I	CTGAAG	1 cut(s)	411
Eco81I	CCTNAGG	1 cut(s)	2025
Eco88I	CYCGRG	1 cut(s)	1166
EcoICRI	GAGCTC	1 cut(s)	328
EcoO109I	RGGNCCY	1 cut(s)	2518
EcoRI	GAATTC	2 cut(s)	1493, 1793
EcoRII	CCWGG	3 cut(s)	1485, 2013, 2520
EcoRV	GATATC	1 cut(s)	975
EcoT14I	CCWWGG	3 cut(s)	2271, 2536, 2594
EcoT38I	GRGCYC	2 cut(s)	330, 2521
ErhI	CCWWGG	3 cut(s)	2271, 2536, 2594
FalI	AAGNNNNNCTT	4 cut(s)	242, 274, 1644, 1676
FaqI	GGGAC	2 cut(s)	722, 1741
FauI	CCCGC	1 cut(s)	1992
FbaI	TGATCA	2 cut(s)	1230, 1414
FokI	GGATG	6 cut(s)	44, 392, 866, 1317, 1469, 2519
FriOI	GRGCYC	2 cut(s)	330, 2521
FspBI	CTAG	8 cut(s)	233, 960, 1010, 1014, 1085, 1409, 1574, 2478
GlaI	GCGC	2 cut(s)	1347, 2392
GsaI	CCCAGC	2 cut(s)	1475, 2106
GsuI	CTGGAG	1 cut(s)	1956
HaeII	RGCGCY	1 cut(s)	2394
HaeIII	GGCC	2 cut(s)	1249, 2519
HapII	CCGG	4 cut(s)	136, 1167, 2556, 2604
HhaI	GCGC	2 cut(s)	1348, 2393
Hin6I	GCGC	2 cut(s)	1346, 2391
HinP1I	GCGC	2 cut(s)	1346, 2391
HincII	GTYRAC	1 cut(s)	1893
HindII	GTYRAC	1 cut(s)	1893
HindIII	AAGCTT	1 cut(s)	565
HpaII	CCGG	4 cut(s)	136, 1167, 2556, 2604
HphI	GGTGA	5 cut(s)	1280, 1711, 1973, 2210, 2343
Hpy166II	GTNNAC	9 cut(s)	162, 256, 352, 454, 774, 1219, 1832, 1893, 1930
Hpy8I	GTNNAC	9 cut(s)	162, 256, 352, 454, 774, 1219, 1832, 1893, 1930
HpyAV	CCTTC	2 cut(s)	368, 1633
HpyCH4III	ACNGT	7 cut(s)	745, 1444, 1708, 1738, 1753, 1804, 2320
HpyCH4IV	ACGT	2 cut(s)	456, 835
HpySE526I	ACGT	2 cut(s)	456, 835
HspAI	GCGC	2 cut(s)	1346, 2391
KroI	GCCGGC	1 cut(s)	135
KroNI	GCCGGC	1 cut(s)	137
Ksp22I	TGATCA	2 cut(s)	1230, 1414
LguI	GCTCTTC	1 cut(s)	198
LmnI	GCTCC	3 cut(s)	58, 176, 1937
LweI	GCATC	9 cut(s)	22, 370, 478, 1021, 1078, 1295, 1572, 2193, 2250
MaeI	CTAG	8 cut(s)	233, 960, 1010, 1014, 1085, 1409, 1574, 2478
MaeII	ACGT	2 cut(s)	456, 835
MaeIII	GTNAC	9 cut(s)	472, 542, 1268, 1961, 2116, 2191, 2298, 2320, 2458
MboII	GAAGA	7 cut(s)	185, 404, 542, 600, 956, 1965, 2462
MfeI	CAATTG	2 cut(s)	2223, 2385
MflI	RGATCY	5 cut(s)	320, 509, 1419, 2470, 2580
MhlI	GDGCHC	6 cut(s)	164, 330, 776, 875, 1396, 2521
MlyI	GAGTC	3 cut(s)	185, 607, 2342
MmeI	TCCRAC	3 cut(s)	33, 1463, 1720
MroNI	GCCGGC	1 cut(s)	135
MseI	TTAA	9 cut(s)	80, 381, 716, 758, 830, 1155, 1317, 1878, 2694
MslI	CAYNNNNRTG	1 cut(s)	779
MspCI	CTTAAG	2 cut(s)	79, 1154
MspI	CCGG	4 cut(s)	136, 1167, 2556, 2604
MspR9I	CCNGG	5 cut(s)	1167, 1168, 1487, 2015, 2522
MunI	CAATTG	2 cut(s)	2223, 2385
Mva1269I	GAATGC	1 cut(s)	756
MvaI	CCWGG	3 cut(s)	1487, 2015, 2522
NaeI	GCCGGC	1 cut(s)	137
NciI	CCSGG	2 cut(s)	1167, 1168
NcoI	CCATGG	1 cut(s)	2271
NgoMIV	GCCGGC	1 cut(s)	135
NheI	GCTAGC	1 cut(s)	1408
NlaIV	GGNNCC	7 cut(s)	629, 874, 1288, 2398, 2519, 2582, 2608
NmuCI	GTSAC	4 cut(s)	472, 1268, 1961, 2320
NspI	RCATGY	6 cut(s)	739, 1587, 1904, 2073, 2148, 2545
NspV	TTCGAA	1 cut(s)	196
OliI	CACNNNNGTG	1 cut(s)	779
PaeI	GCATGC	2 cut(s)	1587, 2545
PagI	TCATGA	1 cut(s)	291
PasI	CCCWGGG	1 cut(s)	2521
PceI	AGGCCT	1 cut(s)	1249
PciI	ACATGT	2 cut(s)	1900, 2069
PciSI	GCTCTTC	1 cut(s)	198
PctI	GAATGC	1 cut(s)	756
PdiI	GCCGGC	1 cut(s)	137
PfeI	GAWTC	8 cut(s)	5, 185, 422, 727, 766, 2289, 2340, 2559
PflFI	GACNNNGTC	1 cut(s)	2488
PflMI	CCANNNNNTGG	2 cut(s)	1515, 2170
PleI	GAGTC	3 cut(s)	184, 607, 2342
PpsI	GAGTC	3 cut(s)	184, 607, 2342
PscI	ACATGT	2 cut(s)	1900, 2069
PshAI	GACNNNNGTC	1 cut(s)	838
PsiI	TTATAA	1 cut(s)	2667
Psp124BI	GAGCTC	1 cut(s)	330
Psp6I	CCWGG	3 cut(s)	1485, 2013, 2520
PspFI	CCCAGC	2 cut(s)	1471, 2102
PspGI	CCWGG	3 cut(s)	1485, 2013, 2520
PspN4I	GGNNCC	7 cut(s)	629, 874, 1288, 2398, 2519, 2582, 2608
PspOMI	GGGCCC	1 cut(s)	2517
PspPI	GGNCC	4 cut(s)	503, 2021, 2517, 2518
PstI	CTGCAG	3 cut(s)	1557, 1819, 2368
PstNI	CAGNNNCTG	4 cut(s)	662, 1343, 2141, 2552
PsuI	RGATCY	5 cut(s)	320, 509, 1419, 2470, 2580
PsyI	GACNNNGTC	1 cut(s)	2488
RsaI	GTAC	6 cut(s)	623, 722, 998, 1572, 1801, 1929
RsaNI	GTAC	6 cut(s)	622, 721, 997, 1571, 1800, 1928
RseI	CAYNNNNRTG	1 cut(s)	779
SacI	GAGCTC	1 cut(s)	330
SapI	GCTCTTC	1 cut(s)	198
SaqAI	TTAA	9 cut(s)	80, 381, 716, 758, 830, 1155, 1317, 1878, 2694
Sau96I	GGNCC	4 cut(s)	503, 2021, 2517, 2518
ScaI	AGTACT	2 cut(s)	998, 1572
SchI	GAGTC	3 cut(s)	185, 607, 2342
ScrFI	CCNGG	5 cut(s)	1167, 1168, 1487, 2015, 2522
SduI	GDGCHC	6 cut(s)	164, 330, 776, 875, 1396, 2521
SfaNI	GCATC	9 cut(s)	22, 370, 478, 1021, 1078, 1295, 1572, 2193, 2250
SfcI	CTRYAG	5 cut(s)	744, 1553, 1614, 1815, 2364
SfuI	TTCGAA	1 cut(s)	196
SinI	GGWCC	2 cut(s)	503, 2021
SmaI	CCCGGG	1 cut(s)	1168
SmiMI	CAYNNNNRTG	1 cut(s)	779
SmlI	CTYRAG	5 cut(s)	79, 239, 608, 640, 1154
SmoI	CTYRAG	5 cut(s)	79, 239, 608, 640, 1154
SpeI	ACTAGT	2 cut(s)	959, 1573
SphI	GCATGC	2 cut(s)	1587, 2545
SseBI	AGGCCT	1 cut(s)	1249
SsiI	CCGC	2 cut(s)	24, 1999
SspMI	CTAG	8 cut(s)	233, 960, 1010, 1014, 1085, 1409, 1574, 2478
SstI	GAGCTC	1 cut(s)	330
StuI	AGGCCT	1 cut(s)	1249
StyD4I	CCNGG	5 cut(s)	1165, 1166, 1485, 2013, 2520
StyI	CCWWGG	3 cut(s)	2271, 2536, 2594
TaaI	ACNGT	7 cut(s)	745, 1444, 1708, 1738, 1753, 1804, 2320
TaiI	ACGT	2 cut(s)	459, 838
TaqI	TCGA	5 cut(s)	196, 287, 2065, 2244, 2494
TaqII	GACCGA	2 cut(s)	1487, 2279
TatI	WGTACW	5 cut(s)	621, 720, 996, 1570, 1799
TfiI	GAWTC	8 cut(s)	5, 185, 422, 727, 766, 2289, 2340, 2559
Tru1I	TTAA	9 cut(s)	80, 381, 716, 758, 830, 1155, 1317, 1878, 2694
Tru9I	TTAA	9 cut(s)	80, 381, 716, 758, 830, 1155, 1317, 1878, 2694
TscAI	CASTG	9 cut(s)	280, 505, 748, 873, 1557, 1737, 1939, 2133, 2581
TseFI	GTSAC	4 cut(s)	472, 1268, 1961, 2320
Tsp45I	GTSAC	4 cut(s)	472, 1268, 1961, 2320
TspGWI	ACGGA	1 cut(s)	169
TspMI	CCCGGG	1 cut(s)	1166
TspRI	CASTG	9 cut(s)	280, 505, 748, 873, 1557, 1737, 1939, 2133, 2581
Tth111I	GACNNNGTC	1 cut(s)	2488
Van91I	CCANNNNNTGG	2 cut(s)	1515, 2170
Vha464I	CTTAAG	2 cut(s)	79, 1154
VneI	GTGCAC	2 cut(s)	160, 772
VpaK11BI	GGWCC	2 cut(s)	503, 2021
XapI	RAATTY	5 cut(s)	107, 1493, 1793, 2036, 2496
XbaI	TCTAGA	1 cut(s)	2477
XceI	RCATGY	6 cut(s)	739, 1587, 1904, 2073, 2148, 2545
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1783
XmaI	CCCGGG	1 cut(s)	1166
XspI	CTAG	8 cut(s)	233, 960, 1010, 1014, 1085, 1409, 1574, 2478
ZrmI	AGTACT	2 cut(s)	998, 1572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.