MD11G1214100.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007495
Physical Location & Seq
Reverse (-)
31271770 .. 31273047
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1214100.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGGCTACGCTTCAATCTTCTTTTTGTTCTTCTTCTCGTTTTTCTTCAGCTACGTTTAGAAACACAAAAGCCACTCTTCACATACCAAAATTCCAAAAATCATCCTCTCTTTCAGTTCCTAGACAGCTCCTTTTGAATGAATTAAATCTAGCAGCCACTTCAACTGATTTAGAACACAACCCAAAATTCGGAAACAAGAAAAAATCAAATCGTAACGAACCAAATTCGGATTCGAATTTGGTGCAAGAGTTGTACGCAGTAATGGAGATTGTGTCTGACAGAGCAGAAATGCATAAGAACATATGTGCTCAGAGGGACAACTGGAACGCCCTACTTTTGAACTCCGTGAACGGAATGACAGCCACGGCGGCGATCATGGCCGGACTCGCATCAGCAGCAGGATCCGGAGCGTCCATTCTGCCCCTCAAGCTCTCTGCTGGCCTCCTCTACATTTCCGTCACCGGGATTCTGCTGGTGATGAACAAAATCCAGCCTTCCCAGCTGGCGGAAGAACAGAGAAACGCTTCCAGGCTGTTCAAACGGCTTCACCAAGAGATCAAAACGACGCTGTTTCTGAAAACTCCGACTTCAAAGGACGTGAAACTTGCAATGGAGAAGGTGCTGGCATTGGACAAGGCCTACCCACTTCCTCTTTTGGGAACCATGATCGAGAAGTTCCCGTCCCACGTGGAGCCCGCTGTGTGGTGGCCGGAGGAGCACGGGACTGGGCATGAGAAGGCGGGCGAGGAGGAGGTAGAGAGGAACGGCTGGGATGAGAAGTTGGAGGAGGAAATGAAGGGCGTGTTGGGTGTGGTGAGAGGAAAAGATGGTGCGGAATACGTGAGGCTGAGCAATGTGGTGCTGAAGGTGAACAAGGTTCTGGCAATGTGTGGGCCAATGCTGACCGTGTTTGCGGCGGCTGGGGCGGGGGTTGTAGGGTGTGATGGGTTCGTCGGGTGGTGGGGTGCGGTGCTGGCTGTGGTGTGTGGGGGCTTGGGTGTGGTGGTGAATAGTTTGGAGCACGGCGGGCAGGTGGGGATGGTGTTTGAGATGTATAGGAGTTCTGCTGGGTTTTTTAAGCTGATGGAGGAGACGATAGAGTCGGCGTTGAAGGAAGGGGAGGTTGGGGGAAAGAGGGAGAATGGGGAGGTGTTCGAAGTGAAAGTGGCGCTTCAGCTCGGAAGGAGCATTTCGGAGCTCAGAGATCTGGCGAGTTCTGAGTCTTTGGCTTCCAGATTGAAGCAGGTCAAAGAGGAGTTTGCAAGCAAGCTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

426

Amino Acids

46.23

Weight (kDa)

7.1

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 84 - 401 3.4e-143 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1021
AasI GACNNNNNNGTC 1 cut(s) 1099
Acc36I ACCTGC 2 cut(s) 1021, 1234
AccIII TCCGGA 1 cut(s) 404
AclWI GGATC 2 cut(s) 396, 409
AcoI YGGCCR 2 cut(s) 378, 707
AcsI RAATTY 4 cut(s) 89, 185, 223, 235
AcuI CTGAAG 3 cut(s) 30, 884, 1157
AcvI CACGTG 1 cut(s) 688
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 5 cut(s) 188, 462, 505, 656, 702
AgsI TTSAA 8 cut(s) 14, 136, 162, 340, 538, 591, 1111, 1240
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 1 cut(s) 527
AjuI GAANNNNNNNTTGG 6 cut(s) 214, 246, 788, 820, 1107, 1139
AluBI AGCT 8 cut(s) 50, 127, 430, 502, 1081, 1177, 1198, 1270
AluI AGCT 8 cut(s) 50, 127, 430, 502, 1081, 1177, 1198, 1270
Alw21I GWGCWC 4 cut(s) 310, 720, 1023, 1200
Alw26I GTCTC 1 cut(s) 1085
AlwI GGATC 2 cut(s) 396, 409
Aor13HI TCCGGA 1 cut(s) 404
AoxI GGCC 5 cut(s) 378, 439, 636, 707, 893
ApeKI GCWGC 2 cut(s) 152, 395
ApoI RAATTY 4 cut(s) 89, 185, 223, 235
Asp700I GAANNNNTTC 1 cut(s) 523
AspLEI GCGC 1 cut(s) 1171
AspS9I GGNCC 1 cut(s) 893
AsuC2I CCSGG 1 cut(s) 463
AsuHPI GGTGA 6 cut(s) 451, 487, 539, 826, 880, 1018
AsuII TTCGAA 2 cut(s) 233, 1155
BamHI GGATCC 1 cut(s) 401
BanII GRGCYC 2 cut(s) 696, 1200
BbrPI CACGTG 1 cut(s) 688
Bbv12I GWGCWC 4 cut(s) 310, 720, 1023, 1200
BbvI GCAGC 2 cut(s) 164, 407
BccI CCATC 4 cut(s) 821, 938, 1033, 1078
BceAI ACGGC 4 cut(s) 381, 557, 781, 1039
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 1 cut(s) 529
BcnI CCSGG 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 1085
BfaI CTAG 2 cut(s) 120, 149
BfoI RGCGCY 1 cut(s) 1172
BfuAI ACCTGC 2 cut(s) 1021, 1234
BglI GCCNNNNNGGC 1 cut(s) 368
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 5 cut(s) 153, 369, 396, 915, 918
BlpI GCTNAGC 1 cut(s) 848
BlsI GCNGC 5 cut(s) 154, 370, 397, 916, 919
Bme1390I CCNGG 2 cut(s) 463, 529
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 3 cut(s) 403, 661, 693
BmrFI CCNGG 2 cut(s) 463, 529
BmrI ACTGGG 1 cut(s) 735
BmsI GCATC 1 cut(s) 398
BmuI ACTGGG 1 cut(s) 735
Bpu1102I GCTNAGC 1 cut(s) 848
Bpu14I TTCGAA 2 cut(s) 233, 1155
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 463
BsaAI YACGTR 2 cut(s) 688, 841
BsaJI CCNNGG 1 cut(s) 363
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 5 cut(s) 188, 462, 505, 656, 702
Bse1I ACTGG 2 cut(s) 326, 730
Bse3DI GCAATG 3 cut(s) 615, 859, 891
BseAI TCCGGA 1 cut(s) 404
BseBI CCWGG 1 cut(s) 529
BseDI CCNNGG 1 cut(s) 363
BseGI GGATG 3 cut(s) 101, 778, 1044
BseLI CCNNNNNNNGG 5 cut(s) 188, 462, 505, 656, 702
BseMI GCAATG 3 cut(s) 615, 859, 891
BseMII CTCAG 4 cut(s) 323, 839, 1209, 1213
BseNI ACTGG 2 cut(s) 326, 730
BseRI GAGGAG 7 cut(s) 434, 728, 761, 764, 800, 1103, 1268
BseXI GCAGC 2 cut(s) 164, 407
BseYI CCCAGC 4 cut(s) 498, 768, 920, 1067
BshFI GGCC 5 cut(s) 380, 441, 638, 709, 895
BsiHKAI GWGCWC 4 cut(s) 310, 720, 1023, 1200
BsiSI CCGG 4 cut(s) 381, 405, 462, 710
BslFI GGGAC 3 cut(s) 329, 667, 736
BslI CCNNNNNNNGG 5 cut(s) 188, 462, 505, 656, 702
BsmAI GTCTC 1 cut(s) 1085
BsmBI CGTCTC 1 cut(s) 1085
BsmFI GGGAC 3 cut(s) 329, 667, 736
BsnI GGCC 5 cut(s) 380, 441, 638, 709, 895
Bsp119I TTCGAA 2 cut(s) 233, 1155
Bsp1286I GDGCHC 5 cut(s) 310, 696, 720, 1023, 1200
Bsp13I TCCGGA 1 cut(s) 404
Bsp143I GATC 5 cut(s) 372, 401, 555, 666, 1204
Bsp1720I GCTNAGC 1 cut(s) 848
BspANI GGCC 5 cut(s) 380, 441, 638, 709, 895
BspCNI CTCAG 4 cut(s) 322, 840, 1210, 1212
BspEI TCCGGA 1 cut(s) 404
BspLI GGNNCC 3 cut(s) 403, 661, 693
BspMI ACCTGC 2 cut(s) 1021, 1234
BspPI GGATC 2 cut(s) 396, 409
BspT104I TTCGAA 2 cut(s) 233, 1155
BsrDI GCAATG 3 cut(s) 615, 859, 891
BsrI ACTGG 2 cut(s) 326, 730
BssECI CCNNGG 1 cut(s) 363
BssMI GATC 5 cut(s) 372, 401, 555, 666, 1204
Bst2UI CCWGG 1 cut(s) 529
Bst4CI ACNGT 1 cut(s) 907
Bst6I CTCTTC 1 cut(s) 81
BstBAI YACGTR 2 cut(s) 688, 841
BstBI TTCGAA 2 cut(s) 233, 1155
BstC8I GCNNGC 9 cut(s) 439, 504, 624, 696, 742, 975, 1028, 1264, 1268
BstDEI CTNAG 4 cut(s) 309, 848, 1199, 1218
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 3 cut(s) 101, 778, 1044
BstH2I RGCGCY 1 cut(s) 1172
BstHHI GCGC 1 cut(s) 1171
BstKTI GATC 5 cut(s) 375, 404, 558, 669, 1207
BstMAI GTCTC 1 cut(s) 1085
BstMBI GATC 5 cut(s) 372, 401, 555, 666, 1204
BstNI CCWGG 1 cut(s) 529
BstSCI CCNGG 2 cut(s) 461, 527
BstV1I GCAGC 2 cut(s) 164, 407
BstX2I RGATCY 2 cut(s) 401, 1204
BstYI RGATCY 2 cut(s) 401, 1204
BsuRI GGCC 5 cut(s) 380, 441, 638, 709, 895
BtgI CCRYGG 1 cut(s) 363
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 3 cut(s) 101, 778, 1044
BveI ACCTGC 2 cut(s) 1021, 1234
Cac8I GCNNGC 9 cut(s) 439, 504, 624, 696, 742, 975, 1028, 1264, 1268
CfoI GCGC 1 cut(s) 1171
Cfr13I GGNCC 1 cut(s) 893
CseI GACGC 2 cut(s) 399, 574
Csp6I GTAC 1 cut(s) 253
CviAII CATG 3 cut(s) 376, 664, 731
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 4 cut(s) 309, 848, 1199, 1218
DpnI GATC 5 cut(s) 374, 403, 557, 668, 1206
DpnII GATC 5 cut(s) 372, 401, 555, 666, 1204
DrdI GACNNNNNNGTC 1 cut(s) 1099
DseDI GACNNNNNNGTC 1 cut(s) 1099
EaeI YGGCCR 2 cut(s) 378, 707
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
EciI GGCGGA 1 cut(s) 521
Ecl136II GAGCTC 1 cut(s) 1198
Eco147I AGGCCT 1 cut(s) 638
Eco24I GRGCYC 2 cut(s) 696, 1200
Eco53kI GAGCTC 1 cut(s) 1198
Eco57I CTGAAG 3 cut(s) 30, 884, 1157
Eco72I CACGTG 1 cut(s) 688
EcoICRI GAGCTC 1 cut(s) 1198
EcoRII CCWGG 1 cut(s) 527
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 696, 1200
Esp3I CGTCTC 1 cut(s) 1085
FaeI CATG 3 cut(s) 379, 667, 734
FaiI YATR 8 cut(s) 83, 294, 302, 304, 377, 665, 732, 1056
FalI AAGNNNNNCTT 4 cut(s) 60, 92, 1155, 1187
FaqI GGGAC 3 cut(s) 329, 667, 736
FatI CATG 3 cut(s) 375, 663, 730
FauI CCCGC 4 cut(s) 703, 733, 919, 1019
FauNDI CATATG 1 cut(s) 302
Fnu4HI GCNGC 5 cut(s) 153, 369, 396, 915, 918
FokI GGATG 3 cut(s) 88, 785, 1051
FriOI GRGCYC 2 cut(s) 696, 1200
Fsp4HI GCNGC 5 cut(s) 153, 369, 396, 915, 918
FspBI CTAG 2 cut(s) 120, 149
GlaI GCGC 1 cut(s) 1170
GluI GCNGC 5 cut(s) 153, 369, 396, 915, 918
GsaI CCCAGC 4 cut(s) 502, 772, 924, 1071
HaeII RGCGCY 1 cut(s) 1172
HaeIII GGCC 5 cut(s) 380, 441, 638, 709, 895
HapII CCGG 4 cut(s) 381, 405, 462, 710
HgaI GACGC 2 cut(s) 399, 574
HhaI GCGC 1 cut(s) 1171
Hin1II CATG 3 cut(s) 379, 667, 734
Hin6I GCGC 1 cut(s) 1169
HinP1I GCGC 1 cut(s) 1169
HinfI GANTC 5 cut(s) 230, 384, 466, 1100, 1220
HpaII CCGG 4 cut(s) 381, 405, 462, 710
HphI GGTGA 6 cut(s) 451, 487, 539, 826, 880, 1018
Hpy166II GTNNAC 2 cut(s) 349, 871
Hpy188III TCNNGA 3 cut(s) 405, 670, 1233
Hpy8I GTNNAC 2 cut(s) 349, 871
Hpy99I CGWCG 2 cut(s) 568, 956
HpyAV CCTTC 8 cut(s) 504, 610, 730, 790, 859, 1105, 1109, 1176
HpyCH4III ACNGT 1 cut(s) 907
HpyCH4IV ACGT 4 cut(s) 53, 597, 687, 840
HpyCH4V TGCA 4 cut(s) 244, 292, 608, 1262
HpyF3I CTNAG 4 cut(s) 309, 848, 1199, 1218
HpySE526I ACGT 4 cut(s) 53, 597, 687, 840
Hsp92II CATG 3 cut(s) 379, 667, 734
HspAI GCGC 1 cut(s) 1169
Kpn2I TCCGGA 1 cut(s) 404
Kzo9I GATC 5 cut(s) 372, 401, 555, 666, 1204
LmnI GCTCC 7 cut(s) 132, 407, 691, 715, 1018, 1185, 1195
Lsp1109I GCAGC 2 cut(s) 164, 407
LweI GCATC 1 cut(s) 398
MaeI CTAG 2 cut(s) 120, 149
MaeII ACGT 4 cut(s) 53, 597, 687, 840
MaeIII GTNAC 2 cut(s) 212, 457
MalI GATC 5 cut(s) 374, 403, 557, 668, 1206
MboI GATC 5 cut(s) 372, 401, 555, 666, 1204
MboII GAAGA 6 cut(s) 9, 21, 24, 36, 68, 521
MflI RGATCY 2 cut(s) 401, 1204
MhlI GDGCHC 5 cut(s) 310, 696, 720, 1023, 1200
MluCI AATT 5 cut(s) 89, 140, 185, 223, 235
MlyI GAGTC 3 cut(s) 378, 1109, 1229
MmeI TCCRAC 2 cut(s) 608, 762
Mph1103I ATGCAT 1 cut(s) 294
MroI TCCGGA 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 523
MseI TTAA 3 cut(s) 143, 1077, 1276
MspA1I CMGCKG 2 cut(s) 502, 698
MspI CCGG 4 cut(s) 381, 405, 462, 710
MspR9I CCNGG 2 cut(s) 463, 529
MvaI CCWGG 1 cut(s) 529
NciI CCSGG 1 cut(s) 463
NdeI CATATG 1 cut(s) 302
NdeII GATC 5 cut(s) 372, 401, 555, 666, 1204
NlaIII CATG 3 cut(s) 379, 667, 734
NlaIV GGNNCC 3 cut(s) 403, 661, 693
NmuCI GTSAC 1 cut(s) 457
NsiI ATGCAT 1 cut(s) 294
NspV TTCGAA 2 cut(s) 233, 1155
PaqCI CACCTGC 1 cut(s) 1021
PceI AGGCCT 1 cut(s) 638
PdmI GAANNNNTTC 1 cut(s) 523
PfeI GAWTC 2 cut(s) 230, 466
PkrI GCNGC 5 cut(s) 154, 370, 397, 916, 919
PleI GAGTC 3 cut(s) 378, 1108, 1228
PmaCI CACGTG 1 cut(s) 688
PmlI CACGTG 1 cut(s) 688
PpsI GAGTC 3 cut(s) 378, 1108, 1228
Ppu21I YACGTR 2 cut(s) 688, 841
Psp124BI GAGCTC 1 cut(s) 1200
Psp6I CCWGG 1 cut(s) 527
PspCI CACGTG 1 cut(s) 688
PspFI CCCAGC 4 cut(s) 498, 768, 920, 1067
PspGI CCWGG 1 cut(s) 527
PspN4I GGNNCC 3 cut(s) 403, 661, 693
PspPI GGNCC 1 cut(s) 893
PsuI RGATCY 2 cut(s) 401, 1204
PvuII CAGCTG 1 cut(s) 502
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SacI GAGCTC 1 cut(s) 1200
SaqAI TTAA 3 cut(s) 143, 1077, 1276
SatI GCNGC 5 cut(s) 153, 369, 396, 915, 918
Sau3AI GATC 5 cut(s) 372, 401, 555, 666, 1204
Sau96I GGNCC 1 cut(s) 893
SchI GAGTC 3 cut(s) 378, 1109, 1229
ScrFI CCNGG 2 cut(s) 463, 529
SduI GDGCHC 5 cut(s) 310, 696, 720, 1023, 1200
SfaNI GCATC 1 cut(s) 398
SfuI TTCGAA 2 cut(s) 233, 1155
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 5 cut(s) 89, 140, 185, 223, 235
SseBI AGGCCT 1 cut(s) 638
SspMI CTAG 2 cut(s) 120, 149
SstI GAGCTC 1 cut(s) 1200
StuI AGGCCT 1 cut(s) 638
StyD4I CCNGG 2 cut(s) 461, 527
TaaI ACNGT 1 cut(s) 907
TaiI ACGT 4 cut(s) 56, 600, 690, 843
TaqI TCGA 3 cut(s) 233, 669, 1155
TasI AATT 5 cut(s) 89, 140, 185, 223, 235
TauI GCSGC 3 cut(s) 371, 917, 920
TfiI GAWTC 2 cut(s) 230, 466
Tru1I TTAA 3 cut(s) 143, 1077, 1276
Tru9I TTAA 3 cut(s) 143, 1077, 1276
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 2 cut(s) 152, 395
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 3 cut(s) 153, 494, 809
TspGWI ACGGA 3 cut(s) 334, 366, 445
XapI RAATTY 4 cut(s) 89, 185, 223, 235
XmnI GAANNNNTTC 1 cut(s) 523
XspI CTAG 2 cut(s) 120, 149
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.