RLG00000033992

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007495
Physical Location & Seq
Forward (+)
35117157 .. 35120590
3434 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033992

Sequence Viewer

Length: 1326 bp
ATGGTTGTCGGGGATGGATTAGGTTACAGGGCAGATGGTTCGTCACGGACGGGAAATGGTGATGACAATCCGGGAGAGTTGGCAGCTTCCTCTAGAAAAATCAAAGCTACTCCCCACTCCCAAAACATTGAATCATCCCGTCTTTCAGTTCCCAGGCTCCTGGTTAATGAACTGAATCAAAGAAATGGGTCACTCAACCTAACAGCCACTTCATCTGAAATAGAAGTGAAACCATTATTTGACAACAGAAAAAGATCAAATCTTGGTGACTCAGTTTCAGATTCAAACTTTGTCCAAGAGCTCTATGCAATAATGGAAATTGTGGCAGATAGAATAGAAATGCACAACAACATAAAGGCTCAGAGGGACAACTGGAATGGTCTGCTTCTCAACTCTGTGAATGGGATGACAGCCACAGCTGCAATCATGGCGGGACTTGCATCTGTCTCTGGATCTGGACCGTCCATTTTGTCTCTCAAGATCTCATCAAGTCTCCTATACATGGCTGTGACAGGGATTGTTCTAGTGATGAACAAGATCCAACCTTCACAGCTAGCAGAGGAACAAAGAAATGCTTCAAGGCTGTTCAAGAAGCTTCATGAAGAAATCAAAACCACTTTGGCTCTCAAAAACCCAGTCACTTCTTATGATGTGAAAGTCACAATGGAAAAGGTTTTGGCTTTGGATAAGGCATACCCACTTCCCTTACTTGGAACCATGATTGAGAAGTTTCCTCAAATTGTGGAGCCAGCTGTGTGGTGGCCTGAGGAAGAACCCAAGTGCCATGATCATAACATGAGTGACAAGTTTGAGAGGAATGGGTGGAATGAGAATCTTGAACAGGAACTGAAGGAGGTTTTGTGCGTAATGAAGGGAAGGGACTCCGCAGAATATGCAAGGCTAAGCAACATGGTGTTGAAGGTCAACAAGGTCTTGGCTTTCTGTGGTCCTTTGCTTACTGGGTCTGCTGCAATTGGGTCAGGTCTTATTGGGTTGCCTGGTTTTGGGTCATGGGGTGCTGTTTTGGGTGTGGTTTTGGGGGCTTTGGGCACTGTGGTTAACAGTTTGGAGCATGGTGGGCAAGTTGGCATGGTGTTTGAGATGTATAGGAGCTCTGCTGGGTTCTTCAAGCTCATGGAGGAGACCATTGAGTCAACTTTGAAGGAAAGAGAGGTTGGGGATAGAGAAAATGGGGAGCTCTTAGAAGTGAAAGTGGCTCTTCAGCTTGGGAGGAGTTTGTCTGAGCTCAGAGACCTTGCAAGTGCTTCGTCTTTGTCTTCAAGAATCCAACAGGCCAAAGAAGAATTTGCAAGCAAGCTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

442

Amino Acids

48.07

Weight (kDa)

5.73

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 101 - 417 3.8e-146 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1150
AciI CCGC 2 cut(s) 431, 885
AclWI GGATC 2 cut(s) 460, 532
AcsI RAATTY 1 cut(s) 1304
AcuI CTGAAG 2 cut(s) 869, 1205
AfiI CCNNNNNNNGG 3 cut(s) 502, 710, 1004
AgsI TTSAA 9 cut(s) 131, 285, 579, 589, 839, 919, 1129, 1162, 1281
AjnI CCWGG 3 cut(s) 152, 159, 997
AjuI GAANNNNNNNTTGG 2 cut(s) 1158, 1190
Alw21I GWGCWC 4 cut(s) 303, 1115, 1200, 1248
Alw26I GTCTC 5 cut(s) 451, 477, 497, 1136, 1245
AlwI GGATC 2 cut(s) 460, 532
AlwNI CAGNNNCTG 1 cut(s) 847
AoxI GGCC 2 cut(s) 761, 1293
ApeKI GCWGC 3 cut(s) 83, 419, 968
ApoI RAATTY 1 cut(s) 1304
ArsI GACNNNNNNTTYG 2 cut(s) 621, 653
Asp700I GAANNNNTTC 1 cut(s) 574
AspS9I GGNCC 2 cut(s) 458, 947
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 71, 278
AsuNHI GCTAGC 1 cut(s) 553
AvaII GGWCC 2 cut(s) 458, 947
AxyI CCTNAGG 1 cut(s) 765
BaeGI GKGCMC 1 cut(s) 1052
BanII GRGCYC 4 cut(s) 303, 1115, 1200, 1248
BbsI GAAGAC 1 cut(s) 1269
Bbv12I GWGCWC 4 cut(s) 303, 1115, 1200, 1248
BbvI GCAGC 3 cut(s) 95, 406, 955
BccI CCATC 2 cut(s) 8, 29
BcgI CGANNNNNNTGC 4 cut(s) 21, 55, 1248, 1282
BciT130I CCWGG 3 cut(s) 154, 161, 999
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 5 cut(s) 451, 477, 497, 1136, 1245
BfaI CTAG 3 cut(s) 93, 524, 554
BglII AGATCT 1 cut(s) 480
BisI GCNGC 3 cut(s) 84, 420, 969
BlpI GCTNAGC 1 cut(s) 902
BlsI GCNGC 3 cut(s) 85, 421, 970
Bme1390I CCNGG 4 cut(s) 72, 154, 161, 999
Bme18I GGWCC 2 cut(s) 458, 947
BmgT120I GGNCC 2 cut(s) 458, 947
BmiI GGNNCC 3 cut(s) 158, 715, 747
BmrFI CCNGG 4 cut(s) 72, 154, 161, 999
BmrI ACTGGG 2 cut(s) 629, 969
BmsI GCATC 1 cut(s) 449
BmtI GCTAGC 1 cut(s) 557
BmuI ACTGGG 2 cut(s) 629, 969
BpiI GAAGAC 1 cut(s) 1269
Bpu1102I GCTNAGC 1 cut(s) 902
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 66
BsaI GGTCTC 2 cut(s) 1136, 1245
BsaJI CCNNGG 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 845, 875
Bsc4I CCNNNNNNNGG 3 cut(s) 502, 710, 1004
Bse1I ACTGG 3 cut(s) 377, 635, 964
Bse21I CCTNAGG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 3 cut(s) 154, 161, 999
BseDI CCNNGG 1 cut(s) 152
BseGI GGATG 3 cut(s) 19, 134, 411
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 3 cut(s) 502, 710, 1004
BseMII CTCAG 5 cut(s) 285, 374, 756, 1233, 1261
BseNI ACTGG 3 cut(s) 377, 635, 964
BseRI GAGGAG 2 cut(s) 1154, 1246
BseSI GKGCMC 1 cut(s) 1052
BseXI GCAGC 3 cut(s) 95, 406, 955
BseYI CCCAGC 1 cut(s) 1118
BshFI GGCC 2 cut(s) 763, 1295
BsiHKAI GWGCWC 4 cut(s) 303, 1115, 1200, 1248
BsiSI CCGG 1 cut(s) 71
BslFI GGGAC 3 cut(s) 380, 447, 893
BslI CCNNNNNNNGG 3 cut(s) 502, 710, 1004
BsmAI GTCTC 5 cut(s) 451, 477, 497, 1136, 1245
BsmFI GGGAC 3 cut(s) 380, 447, 893
BsnI GGCC 2 cut(s) 763, 1295
Bso31I GGTCTC 2 cut(s) 1136, 1245
Bsp1286I GDGCHC 5 cut(s) 303, 1052, 1115, 1200, 1248
Bsp143I GATC 5 cut(s) 254, 452, 480, 537, 787
Bsp1720I GCTNAGC 1 cut(s) 902
BspACI CCGC 2 cut(s) 431, 885
BspANI GGCC 2 cut(s) 763, 1295
BspCNI CTCAG 5 cut(s) 284, 373, 757, 1234, 1260
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 3 cut(s) 158, 715, 747
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 2 cut(s) 460, 532
BspQI GCTCTTC 1 cut(s) 1224
BspTNI GGTCTC 2 cut(s) 1136, 1245
BsrI ACTGG 3 cut(s) 377, 635, 964
BssECI CCNNGG 1 cut(s) 152
BssMI GATC 5 cut(s) 254, 452, 480, 537, 787
Bst2UI CCWGG 3 cut(s) 154, 161, 999
Bst4CI ACNGT 3 cut(s) 462, 1054, 1064
Bst6I CTCTTC 1 cut(s) 1224
BstAPI GCANNNNNTGC 1 cut(s) 893
BstC8I GCNNGC 4 cut(s) 555, 750, 1312, 1316
BstDEI CTNAG 7 cut(s) 271, 360, 765, 902, 1201, 1242, 1247
BstF5I GGATG 3 cut(s) 19, 134, 411
BstKTI GATC 5 cut(s) 257, 455, 483, 540, 790
BstMAI GTCTC 5 cut(s) 451, 477, 497, 1136, 1245
BstMBI GATC 5 cut(s) 254, 452, 480, 537, 787
BstMWI GCNNNNNNNGC 5 cut(s) 419, 428, 437, 893, 1078
BstNI CCWGG 3 cut(s) 154, 161, 999
BstSCI CCNGG 4 cut(s) 70, 152, 159, 997
BstSLI GKGCMC 1 cut(s) 1052
BstV1I GCAGC 3 cut(s) 95, 406, 955
BstV2I GAAGAC 1 cut(s) 1269
BstX2I RGATCY 3 cut(s) 452, 480, 537
BstXI CCANNNNNNTGG 2 cut(s) 160, 756
BstYI RGATCY 3 cut(s) 452, 480, 537
Bsu36I CCTNAGG 1 cut(s) 765
BsuRI GGCC 2 cut(s) 763, 1295
BtsCI GGATG 3 cut(s) 19, 134, 411
BtsIMutI CAGTG 1 cut(s) 1050
Cac8I GCNNGC 4 cut(s) 555, 750, 1312, 1316
CaiI CAGNNNCTG 1 cut(s) 847
CciI TCATGA 1 cut(s) 598
Cfr13I GGNCC 2 cut(s) 458, 947
DdeI CTNAG 7 cut(s) 271, 360, 765, 902, 1201, 1242, 1247
DpnI GATC 5 cut(s) 256, 454, 482, 539, 789
DpnII GATC 5 cut(s) 254, 452, 480, 537, 787
DrdI GACNNNNNNGTC 1 cut(s) 1150
DseDI GACNNNNNNGTC 1 cut(s) 1150
Eam1104I CTCTTC 1 cut(s) 1224
EarI CTCTTC 1 cut(s) 1224
Ecl136II GAGCTC 4 cut(s) 301, 1113, 1198, 1246
Eco24I GRGCYC 4 cut(s) 303, 1115, 1200, 1248
Eco31I GGTCTC 2 cut(s) 1136, 1245
Eco47I GGWCC 2 cut(s) 458, 947
Eco53kI GAGCTC 4 cut(s) 301, 1113, 1198, 1246
Eco57I CTGAAG 2 cut(s) 869, 1205
Eco81I CCTNAGG 1 cut(s) 765
EcoICRI GAGCTC 4 cut(s) 301, 1113, 1198, 1246
EcoRII CCWGG 3 cut(s) 152, 159, 997
EcoT38I GRGCYC 4 cut(s) 303, 1115, 1200, 1248
FalI AAGNNNNNCTT 4 cut(s) 559, 591, 1203, 1235
FaqI GGGAC 3 cut(s) 380, 447, 893
FauI CCCGC 1 cut(s) 424
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 3 cut(s) 84, 420, 969
FokI GGATG 3 cut(s) 26, 121, 418
FriOI GRGCYC 4 cut(s) 303, 1115, 1200, 1248
Fsp4HI GCNGC 3 cut(s) 84, 420, 969
FspBI CTAG 3 cut(s) 93, 524, 554
GluI GCNGC 3 cut(s) 84, 420, 969
GsaI CCCAGC 1 cut(s) 1122
HaeIII GGCC 2 cut(s) 763, 1295
HapII CCGG 1 cut(s) 71
HincII GTYRAC 3 cut(s) 925, 1060, 1155
HindII GTYRAC 3 cut(s) 925, 1060, 1155
HindIII AAGCTT 2 cut(s) 593, 1316
HinfI GANTC 8 cut(s) 131, 175, 269, 281, 832, 881, 1151, 1284
HpaI GTTAAC 1 cut(s) 1060
HpaII CCGG 1 cut(s) 71
HphI GGTGA 2 cut(s) 71, 278
Hpy166II GTNNAC 3 cut(s) 925, 1060, 1155
Hpy188I TCNGA 5 cut(s) 217, 280, 363, 1243, 1250
Hpy188III TCNNGA 8 cut(s) 93, 450, 456, 478, 589, 599, 836, 1281
Hpy8I GTNNAC 3 cut(s) 925, 1060, 1155
HpyAV CCTTC 6 cut(s) 555, 844, 865, 870, 913, 1156
HpyCH4III ACNGT 3 cut(s) 462, 1054, 1064
HpyCH4V TGCA 8 cut(s) 308, 343, 422, 440, 896, 971, 1259, 1310
HpyF10VI GCNNNNNNNGC 5 cut(s) 419, 428, 437, 893, 1078
HpyF3I CTNAG 7 cut(s) 271, 360, 765, 902, 1201, 1242, 1247
Ksp22I TGATCA 1 cut(s) 787
KspAI GTTAAC 1 cut(s) 1060
Kzo9I GATC 5 cut(s) 254, 452, 480, 537, 787
LguI GCTCTTC 1 cut(s) 1224
LmnI GCTCC 5 cut(s) 162, 745, 1069, 1110, 1195
Lsp1109I GCAGC 3 cut(s) 95, 406, 955
LweI GCATC 1 cut(s) 449
MaeI CTAG 3 cut(s) 93, 524, 554
MaeIII GTNAC 8 cut(s) 23, 42, 189, 266, 508, 637, 658, 800
MalI GATC 5 cut(s) 256, 454, 482, 539, 789
MboI GATC 5 cut(s) 254, 452, 480, 537, 787
MboII GAAGA 6 cut(s) 614, 782, 1117, 1211, 1269, 1313
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 3 cut(s) 452, 480, 537
MhlI GDGCHC 5 cut(s) 303, 1052, 1115, 1200, 1248
MluCI AATT 4 cut(s) 318, 738, 972, 1304
MlyI GAGTC 3 cut(s) 263, 875, 1160
MmeI TCCRAC 2 cut(s) 565, 1312
MroXI GAANNNNTTC 1 cut(s) 574
MseI TTAA 2 cut(s) 165, 1059
MslI CAYNNNNRTG 1 cut(s) 506
MspA1I CMGCKG 2 cut(s) 419, 752
MspI CCGG 1 cut(s) 71
MspR9I CCNGG 4 cut(s) 72, 154, 161, 999
MunI CAATTG 1 cut(s) 972
MvaI CCWGG 3 cut(s) 154, 161, 999
MwoI GCNNNNNNNGC 5 cut(s) 419, 428, 437, 893, 1078
NciI CCSGG 1 cut(s) 72
NdeII GATC 5 cut(s) 254, 452, 480, 537, 787
NheI GCTAGC 1 cut(s) 553
NlaIV GGNNCC 3 cut(s) 158, 715, 747
NmuCI GTSAC 7 cut(s) 42, 189, 266, 508, 637, 658, 800
PagI TCATGA 1 cut(s) 598
PciSI GCTCTTC 1 cut(s) 1224
PdmI GAANNNNTTC 1 cut(s) 574
PfeI GAWTC 5 cut(s) 131, 175, 281, 832, 1284
PfoI TCCNGGA 1 cut(s) 70
PkrI GCNGC 3 cut(s) 85, 421, 970
PleI GAGTC 3 cut(s) 263, 875, 1159
PpsI GAGTC 3 cut(s) 263, 875, 1159
Psp124BI GAGCTC 4 cut(s) 303, 1115, 1200, 1248
Psp6I CCWGG 3 cut(s) 152, 159, 997
PspFI CCCAGC 1 cut(s) 1118
PspGI CCWGG 3 cut(s) 152, 159, 997
PspN4I GGNNCC 3 cut(s) 158, 715, 747
PspPI GGNCC 2 cut(s) 458, 947
PstNI CAGNNNCTG 1 cut(s) 847
PsuI RGATCY 3 cut(s) 452, 480, 537
PvuII CAGCTG 2 cut(s) 419, 752
RseI CAYNNNNRTG 1 cut(s) 506
SacI GAGCTC 4 cut(s) 303, 1115, 1200, 1248
SapI GCTCTTC 1 cut(s) 1224
SaqAI TTAA 2 cut(s) 165, 1059
SatI GCNGC 3 cut(s) 84, 420, 969
Sau3AI GATC 5 cut(s) 254, 452, 480, 537, 787
Sau96I GGNCC 2 cut(s) 458, 947
SchI GAGTC 3 cut(s) 263, 875, 1160
ScrFI CCNGG 4 cut(s) 72, 154, 161, 999
SduI GDGCHC 5 cut(s) 303, 1052, 1115, 1200, 1248
SfaNI GCATC 1 cut(s) 449
SinI GGWCC 2 cut(s) 458, 947
SmiMI CAYNNNNRTG 1 cut(s) 506
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 4 cut(s) 318, 738, 972, 1304
SsiI CCGC 2 cut(s) 431, 885
SspMI CTAG 3 cut(s) 93, 524, 554
SstI GAGCTC 4 cut(s) 303, 1115, 1200, 1248
StyD4I CCNGG 4 cut(s) 70, 152, 159, 997
TaaI ACNGT 3 cut(s) 462, 1054, 1064
TasI AATT 4 cut(s) 318, 738, 972, 1304
TfiI GAWTC 5 cut(s) 131, 175, 281, 832, 1284
Tru1I TTAA 2 cut(s) 165, 1059
Tru9I TTAA 2 cut(s) 165, 1059
TscAI CASTG 1 cut(s) 1057
TseFI GTSAC 7 cut(s) 42, 189, 266, 508, 637, 658, 800
TseI GCWGC 3 cut(s) 83, 419, 968
Tsp45I GTSAC 7 cut(s) 42, 189, 266, 508, 637, 658, 800
TspDTI ATGAA 6 cut(s) 183, 201, 545, 587, 615, 884
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 1 cut(s) 1057
VpaK11BI GGWCC 2 cut(s) 458, 947
XapI RAATTY 1 cut(s) 1304
XbaI TCTAGA 1 cut(s) 92
XcmI CCANNNNNNNNNTGG 1 cut(s) 756
XmnI GAANNNNTTC 1 cut(s) 574
XspI CTAG 3 cut(s) 93, 524, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.