Rroxscaffold_1G00040020

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007495
Physical Location & Seq
Reverse (-)
57661635 .. 57663454
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040020.1

Sequence Viewer

Length: 1287 bp
ATGGCCACTATTCAAGCTTCTTTTTGTTCTGCTTCATTTTCTTCAGCTTCCTCTAGAAAAACCAAAGTTACTCTCCACTCCCAAAACTTTGAATCATCGCATCTTTCAGTTCCCAGGCTCCTGGTTAATGAACTGAATCAAAGAAATGGGTCACTCAACCTAACAGCCACTTCATCTGAAATAGAAGTGAAACCATTATTTGACAACAGAAAAAGATCAAATCTCGGTGACTCAGTTTCAGATTCAAATTTTGTCCAAGAGCTCTATGCAATAATGGAGATTGTGGCAGATAGAATAGAAATGCACAACAACATAAAGGCTCAGAGGGACAACTGGAATGGTCTGCTTCTTAACTCTGTGAATGGGATGACAGCCACAGCTGCAATCATGGCGGGACTTGCATCGGTCTCTGGATCTGGACCGTCCATTTTGTCTCTCAAGATCTCATCAAGTCTCCTATACATGGCTGTGACTGGGATTGTTCTAGTGATGAACAAGATCCAACCTTCACAGCTAGCAGAGGAACAAAGAAATGCTTCAAGGCTGTTCAAGAAGCTTCATGAAGAAATCAAAACCACTTTGGCTCTCAAAAACCCAGTTACTTCTTATGATGTGAAAGTCACAATGGGAAAGGTTTTGGCTTTGGATAAGGCATACCCACTTCCCTTACTTGGAACCATGATTGAGAAGTTTCCTCAAATTGTGGAGCCAGCTGTGTGGTGGCCTGAGGAAGAACCCAAGTGCCATGATCATAACATGAGTGACAAGTTTGAGAGGAATGGGTGGAATGAGAATCTTGAAGAGGAACTGAAGGAGGTTTTGTGCGTAATGAAGGGAAGGGACTCCGCAGAATATGCAAGGCTAAGCAACATGGTCTTGAAGGTCAACAAGGTCTTGGCTTTCTGTGGTCCTTTGCTTACTGGGTCTGCTGCAATTGGGTCAGGTCTTATTGGGTTGCCTGGTTTTGGGTCATGGGGTGCTGTTTTGGGTGTGGTTTTGGGGGCTTTGGGCACTGTGGTTAACAGTTTGGAGCATGGAGGGCAAGTTGGAATGGTGTTTGAGATGTATAGGAGCTCTGCTGGGTTCTTCAAGCTCATGGAGGAGACCATTGAGTCAACTTTGAAGGAAAGAGAGGTTGGGGATAGAGAAAATGGGGAGCTCTTTGAAGTGAAAGTGGCTCTACAGCTTGGGAGGAGTTTGTCTGAGCTCAGAGACCTTGCAAGTGCTTCATCTTTGTCTTCAAGAATTCAACAGGCCAAAGAAGAATTTGCAAGCAAACTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

428

Amino Acids

46.84

Weight (kDa)

6.07

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 88 - 404 4e-146 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1111
AciI CCGC 2 cut(s) 392, 846
AclWI GGATC 2 cut(s) 421, 493
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 247, 1245, 1265
AcuI CTGAAG 2 cut(s) 27, 830
AfiI CCNNNNNNNGG 3 cut(s) 463, 671, 965
AjnI CCWGG 3 cut(s) 113, 120, 958
AjuI GAANNNNNNNTTGG 2 cut(s) 1119, 1151
Alw21I GWGCWC 4 cut(s) 264, 1076, 1161, 1209
Alw26I GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
AlwI GGATC 2 cut(s) 421, 493
AoxI GGCC 3 cut(s) 3, 722, 1254
ApeKI GCWGC 2 cut(s) 380, 929
ApoI RAATTY 3 cut(s) 247, 1245, 1265
Asp700I GAANNNNTTC 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 419, 908
AsuHPI GGTGA 1 cut(s) 239
AsuNHI GCTAGC 1 cut(s) 514
AvaII GGWCC 2 cut(s) 419, 908
AxyI CCTNAGG 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 1013
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 4 cut(s) 264, 1076, 1161, 1209
BbsI GAAGAC 1 cut(s) 1230
Bbv12I GWGCWC 4 cut(s) 264, 1076, 1161, 1209
BbvI GCAGC 2 cut(s) 367, 916
BciT130I CCWGG 3 cut(s) 115, 122, 960
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
BfaI CTAG 3 cut(s) 54, 485, 515
BfmI CTRYAG 1 cut(s) 1181
BglII AGATCT 1 cut(s) 441
BisI GCNGC 2 cut(s) 381, 930
BlpI GCTNAGC 1 cut(s) 863
BlsI GCNGC 2 cut(s) 382, 931
Bme1390I CCNGG 3 cut(s) 115, 122, 960
Bme18I GGWCC 2 cut(s) 419, 908
BmgT120I GGNCC 2 cut(s) 419, 908
BmiI GGNNCC 3 cut(s) 119, 676, 708
BmrFI CCNGG 3 cut(s) 115, 122, 960
BmrI ACTGGG 3 cut(s) 483, 590, 930
BmsI GCATC 2 cut(s) 109, 410
BmtI GCTAGC 1 cut(s) 518
BmuI ACTGGG 3 cut(s) 483, 590, 930
BpiI GAAGAC 1 cut(s) 1230
Bpu1102I GCTNAGC 1 cut(s) 863
BpuEI CTTGAG 1 cut(s) 422
BsaI GGTCTC 3 cut(s) 412, 1097, 1206
BsaJI CCNNGG 1 cut(s) 113
BsaXI ACNNNNNCTCC 4 cut(s) 806, 836, 1029, 1059
Bsc4I CCNNNNNNNGG 3 cut(s) 463, 671, 965
Bse1I ACTGG 4 cut(s) 338, 478, 596, 925
Bse21I CCTNAGG 1 cut(s) 726
BseBI CCWGG 3 cut(s) 115, 122, 960
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 3 cut(s) 463, 671, 965
BseMII CTCAG 5 cut(s) 246, 335, 717, 1194, 1222
BseNI ACTGG 4 cut(s) 338, 478, 596, 925
BseRI GAGGAG 2 cut(s) 1115, 1207
BseSI GKGCMC 1 cut(s) 1013
BseXI GCAGC 2 cut(s) 367, 916
BseYI CCCAGC 1 cut(s) 1079
BshFI GGCC 3 cut(s) 5, 724, 1256
BsiHKAI GWGCWC 4 cut(s) 264, 1076, 1161, 1209
BslFI GGGAC 3 cut(s) 341, 408, 854
BslI CCNNNNNNNGG 3 cut(s) 463, 671, 965
BsmAI GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
BsmFI GGGAC 3 cut(s) 341, 408, 854
BsnI GGCC 3 cut(s) 5, 724, 1256
Bso31I GGTCTC 3 cut(s) 412, 1097, 1206
Bsp1286I GDGCHC 5 cut(s) 264, 1013, 1076, 1161, 1209
Bsp143I GATC 5 cut(s) 215, 413, 441, 498, 748
Bsp1720I GCTNAGC 1 cut(s) 863
BspACI CCGC 2 cut(s) 392, 846
BspANI GGCC 3 cut(s) 5, 724, 1256
BspCNI CTCAG 5 cut(s) 245, 334, 718, 1195, 1221
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 3 cut(s) 119, 676, 708
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 2 cut(s) 421, 493
BspTNI GGTCTC 3 cut(s) 412, 1097, 1206
BsrI ACTGG 4 cut(s) 338, 478, 596, 925
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 5 cut(s) 215, 413, 441, 498, 748
Bst2UI CCWGG 3 cut(s) 115, 122, 960
Bst4CI ACNGT 3 cut(s) 423, 1015, 1025
Bst6I CTCTTC 1 cut(s) 795
BstAPI GCANNNNNTGC 1 cut(s) 854
BstC8I GCNNGC 3 cut(s) 516, 711, 1273
BstDEI CTNAG 6 cut(s) 232, 321, 726, 863, 1203, 1208
BstF5I GGATG 1 cut(s) 372
BstKTI GATC 5 cut(s) 218, 416, 444, 501, 751
BstMAI GTCTC 5 cut(s) 412, 438, 458, 1097, 1206
BstMBI GATC 5 cut(s) 215, 413, 441, 498, 748
BstMWI GCNNNNNNNGC 5 cut(s) 380, 389, 398, 854, 1039
BstNI CCWGG 3 cut(s) 115, 122, 960
BstSCI CCNGG 3 cut(s) 113, 120, 958
BstSFI CTRYAG 1 cut(s) 1181
BstSLI GKGCMC 1 cut(s) 1013
BstV1I GCAGC 2 cut(s) 367, 916
BstV2I GAAGAC 1 cut(s) 1230
BstX2I RGATCY 3 cut(s) 413, 441, 498
BstXI CCANNNNNNTGG 2 cut(s) 121, 717
BstYI RGATCY 3 cut(s) 413, 441, 498
Bsu36I CCTNAGG 1 cut(s) 726
BsuRI GGCC 3 cut(s) 5, 724, 1256
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 1011
Cac8I GCNNGC 3 cut(s) 516, 711, 1273
CciI TCATGA 1 cut(s) 559
Cfr13I GGNCC 2 cut(s) 419, 908
DdeI CTNAG 6 cut(s) 232, 321, 726, 863, 1203, 1208
DpnI GATC 5 cut(s) 217, 415, 443, 500, 750
DpnII GATC 5 cut(s) 215, 413, 441, 498, 748
DrdI GACNNNNNNGTC 1 cut(s) 1111
DseDI GACNNNNNNGTC 1 cut(s) 1111
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 795
EarI CTCTTC 1 cut(s) 795
Ecl136II GAGCTC 4 cut(s) 262, 1074, 1159, 1207
Eco24I GRGCYC 4 cut(s) 264, 1076, 1161, 1209
Eco31I GGTCTC 3 cut(s) 412, 1097, 1206
Eco47I GGWCC 2 cut(s) 419, 908
Eco53kI GAGCTC 4 cut(s) 262, 1074, 1159, 1207
Eco57I CTGAAG 2 cut(s) 27, 830
Eco81I CCTNAGG 1 cut(s) 726
EcoICRI GAGCTC 4 cut(s) 262, 1074, 1159, 1207
EcoRI GAATTC 1 cut(s) 1245
EcoRII CCWGG 3 cut(s) 113, 120, 958
EcoT38I GRGCYC 4 cut(s) 264, 1076, 1161, 1209
FalI AAGNNNNNCTT 2 cut(s) 520, 552
FaqI GGGAC 3 cut(s) 341, 408, 854
FauI CCCGC 1 cut(s) 385
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 2 cut(s) 381, 930
FokI GGATG 1 cut(s) 379
FriOI GRGCYC 4 cut(s) 264, 1076, 1161, 1209
Fsp4HI GCNGC 2 cut(s) 381, 930
FspBI CTAG 3 cut(s) 54, 485, 515
GluI GCNGC 2 cut(s) 381, 930
GsaI CCCAGC 1 cut(s) 1083
HaeIII GGCC 3 cut(s) 5, 724, 1256
HincII GTYRAC 3 cut(s) 886, 1021, 1116
HindII GTYRAC 3 cut(s) 886, 1021, 1116
HindIII AAGCTT 2 cut(s) 15, 554
HinfI GANTC 7 cut(s) 92, 136, 230, 242, 793, 842, 1112
HpaI GTTAAC 1 cut(s) 1021
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 3 cut(s) 886, 1021, 1116
Hpy188I TCNGA 5 cut(s) 178, 241, 324, 1204, 1211
Hpy188III TCNNGA 9 cut(s) 54, 411, 417, 439, 550, 560, 797, 877, 1242
Hpy8I GTNNAC 3 cut(s) 886, 1021, 1116
HpyAV CCTTC 6 cut(s) 516, 805, 826, 831, 874, 1117
HpyCH4III ACNGT 3 cut(s) 423, 1015, 1025
HpyCH4V TGCA 8 cut(s) 269, 304, 383, 401, 857, 932, 1220, 1271
HpyF10VI GCNNNNNNNGC 5 cut(s) 380, 389, 398, 854, 1039
HpyF3I CTNAG 6 cut(s) 232, 321, 726, 863, 1203, 1208
Ksp22I TGATCA 1 cut(s) 748
KspAI GTTAAC 1 cut(s) 1021
Kzo9I GATC 5 cut(s) 215, 413, 441, 498, 748
LmnI GCTCC 5 cut(s) 123, 706, 1030, 1071, 1156
Lsp1109I GCAGC 2 cut(s) 367, 916
LweI GCATC 2 cut(s) 109, 410
MaeI CTAG 3 cut(s) 54, 485, 515
MaeIII GTNAC 7 cut(s) 67, 150, 227, 469, 598, 619, 761
MalI GATC 5 cut(s) 217, 415, 443, 500, 750
MboI GATC 5 cut(s) 215, 413, 441, 498, 748
MboII GAAGA 7 cut(s) 33, 575, 743, 812, 1078, 1230, 1274
MfeI CAATTG 1 cut(s) 933
MflI RGATCY 3 cut(s) 413, 441, 498
MhlI GDGCHC 5 cut(s) 264, 1013, 1076, 1161, 1209
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 247, 699, 933, 1245, 1265
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 224, 836, 1121
MmeI TCCRAC 2 cut(s) 526, 1027
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 535
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 126, 351, 1020
MslI CAYNNNNRTG 1 cut(s) 467
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 380, 713
MspR9I CCNGG 3 cut(s) 115, 122, 960
MunI CAATTG 1 cut(s) 933
MvaI CCWGG 3 cut(s) 115, 122, 960
MwoI GCNNNNNNNGC 5 cut(s) 380, 389, 398, 854, 1039
NdeII GATC 5 cut(s) 215, 413, 441, 498, 748
NheI GCTAGC 1 cut(s) 514
NlaIV GGNNCC 3 cut(s) 119, 676, 708
NmuCI GTSAC 5 cut(s) 150, 227, 469, 619, 761
PagI TCATGA 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 4 cut(s) 92, 136, 242, 793
PkrI GCNGC 2 cut(s) 382, 931
PleI GAGTC 3 cut(s) 224, 836, 1120
PpsI GAGTC 3 cut(s) 224, 836, 1120
Psp124BI GAGCTC 4 cut(s) 264, 1076, 1161, 1209
Psp6I CCWGG 3 cut(s) 113, 120, 958
PspFI CCCAGC 1 cut(s) 1079
PspGI CCWGG 3 cut(s) 113, 120, 958
PspN4I GGNNCC 3 cut(s) 119, 676, 708
PspPI GGNCC 2 cut(s) 419, 908
PsuI RGATCY 3 cut(s) 413, 441, 498
PvuII CAGCTG 2 cut(s) 380, 713
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 4 cut(s) 264, 1076, 1161, 1209
SaqAI TTAA 3 cut(s) 126, 351, 1020
SatI GCNGC 2 cut(s) 381, 930
Sau3AI GATC 5 cut(s) 215, 413, 441, 498, 748
Sau96I GGNCC 2 cut(s) 419, 908
SchI GAGTC 3 cut(s) 224, 836, 1121
ScrFI CCNGG 3 cut(s) 115, 122, 960
SduI GDGCHC 5 cut(s) 264, 1013, 1076, 1161, 1209
SfaNI GCATC 2 cut(s) 109, 410
SfcI CTRYAG 1 cut(s) 1181
SinI GGWCC 2 cut(s) 419, 908
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 5 cut(s) 247, 699, 933, 1245, 1265
SsiI CCGC 2 cut(s) 392, 846
SspMI CTAG 3 cut(s) 54, 485, 515
SstI GAGCTC 4 cut(s) 264, 1076, 1161, 1209
StyD4I CCNGG 3 cut(s) 113, 120, 958
TaaI ACNGT 3 cut(s) 423, 1015, 1025
TaqII GACCGA 1 cut(s) 394
TasI AATT 5 cut(s) 247, 699, 933, 1245, 1265
TfiI GAWTC 4 cut(s) 92, 136, 242, 793
Tru1I TTAA 3 cut(s) 126, 351, 1020
Tru9I TTAA 3 cut(s) 126, 351, 1020
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 5 cut(s) 150, 227, 469, 619, 761
TseI GCWGC 2 cut(s) 380, 929
Tsp45I GTSAC 5 cut(s) 150, 227, 469, 619, 761
TspDTI ATGAA 8 cut(s) 24, 144, 162, 506, 548, 576, 845, 1218
TspRI CASTG 1 cut(s) 1018
VpaK11BI GGWCC 2 cut(s) 419, 908
XapI RAATTY 3 cut(s) 247, 1245, 1265
XbaI TCTAGA 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 3 cut(s) 54, 485, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.