Rorug05G0185900

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007495
Physical Location & Seq
Forward (+)
18006630 .. 18007238
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0185900.1

Sequence Viewer

Length: 531 bp
ATGGCCGAGAACAAGGCCCGCTTCATGTTCGTCAACACCAACTCCCTCTGGGACAAAATCGTCGAACAAATGACCAAGAAGATCGGCTACTACTCTCCCTCCATGAACACTCTCTGGCGCACCGGCAGCTTCCTCACCAATAGTCACAGCCCTAAGAAGTTCAGGTCTCGCCGGAAGAAGATCGTCTTCGGACCGACTCAGCCGCCGGACTGCCTCGTGGTTTTCGACTCCGAGAGGAAGTCGTCGGTGATTCTCGAGGCGCATAGGTTGCAGATTCCGATTGTTTCGCTTGTGGACTCGGACATGCCTATCGAGTACTACAACAAGATCACGTATCCGATTCCGTGTAATTCCTCGGTGCAATTTGTGTACCTGTTCTGCAATTTGATCACCAAGACGTTCATGCTTCAGCAGAAGAAGAGCGGCGCCGTTTCGGCCGAAATTGAGACTAGTGAGCGAGTGGGGGTTATAGAGGAGGCTAAGAGCGCAAAGAAGGATGAGGTGCTTGTGGTTCAATCAATTAAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

176

Amino Acids

20.1

Weight (kDa)

9.42

Isoelectric Point (pI)

51.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S2 PF00318 67 - 133 7e-11 Ribosomal protein S2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 425
AccBSI CCGCTC 1 cut(s) 423
AciI CCGC 3 cut(s) 19, 203, 423
AcoI YGGCCR 2 cut(s) 3, 435
AcuI CTGAAG 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 2 cut(s) 317, 371
AgsI TTSAA 1 cut(s) 515
AhlI ACTAGT 1 cut(s) 449
AluBI AGCT 1 cut(s) 129
AluI AGCT 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 171, 440
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 3 cut(s) 3, 15, 435
ApeKI GCWGC 1 cut(s) 126
AspLEI GCGC 4 cut(s) 120, 262, 428, 488
AspS9I GGNCC 2 cut(s) 16, 191
AsuHPI GGTGA 3 cut(s) 127, 259, 382
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 191
BanI GGYRCC 1 cut(s) 425
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 138
BceAI ACGGC 1 cut(s) 413
BciVI GTATCC 1 cut(s) 345
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 2 cut(s) 171, 440
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 429
BfuI GTATCC 1 cut(s) 345
BglI GCCNNNNNGGC 1 cut(s) 434
BisI GCNGC 3 cut(s) 127, 203, 424
BlsI GCNGC 3 cut(s) 128, 204, 425
BmcAI AGTACT 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 2 cut(s) 16, 191
BmiI GGNNCC 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 354
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 83, 113
Bse118I RCCGGY 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 502
BseMII CTCAG 1 cut(s) 212
BseRI GAGGAG 1 cut(s) 488
BseX3I CGGCCG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 438
BshFI GGCC 3 cut(s) 5, 17, 437
BshNI GGYRCC 1 cut(s) 425
BsiEI CGRYCG 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 3 cut(s) 123, 172, 206
BslFI GGGAC 1 cut(s) 65
BsmAI GTCTC 2 cut(s) 171, 440
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 3 cut(s) 5, 17, 437
Bso31I GGTCTC 1 cut(s) 171
BsoBI CYCGRG 1 cut(s) 254
Bsp143I GATC 4 cut(s) 81, 180, 327, 387
BspACI CCGC 3 cut(s) 19, 203, 423
BspANI GGCC 3 cut(s) 5, 17, 437
BspCNI CTCAG 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 427
BspQI GCTCTTC 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 171
BsrBI CCGCTC 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 4 cut(s) 81, 180, 327, 387
BssNI GRCGYC 1 cut(s) 426
BssSI CACGAG 1 cut(s) 215
Bst2BI CACGAG 1 cut(s) 215
Bst6I CTCTTC 1 cut(s) 413
BstACI GRCGYC 1 cut(s) 426
BstAPI GCANNNNNTGC 1 cut(s) 268
BstBAI YACGTR 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 3 cut(s) 153, 198, 480
BstF5I GGATG 1 cut(s) 502
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 4 cut(s) 120, 262, 428, 488
BstKTI GATC 4 cut(s) 84, 183, 330, 390
BstMAI GTCTC 2 cut(s) 171, 440
BstMBI GATC 4 cut(s) 81, 180, 327, 387
BstMCI CGRYCG 1 cut(s) 438
BstMWI GCNNNNNNNGC 4 cut(s) 126, 268, 434, 485
BstNSI RCATGY 1 cut(s) 307
BstV1I GCAGC 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 178
BstZI CGGCCG 1 cut(s) 435
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 3 cut(s) 5, 17, 437
BtsCI GGATG 1 cut(s) 502
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 4 cut(s) 120, 262, 428, 488
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 16, 191
CpoI CGGWCCG 1 cut(s) 191
Csp6I GTAC 2 cut(s) 316, 370
CspI CGGWCCG 1 cut(s) 191
CviAII CATG 4 cut(s) 25, 103, 304, 403
CviJI RGCY 8 cut(s) 5, 17, 87, 129, 150, 202, 437, 479
CviKI_1 RGCY 8 cut(s) 5, 17, 87, 129, 150, 202, 437, 479
CviQI GTAC 2 cut(s) 316, 370
DdeI CTNAG 3 cut(s) 153, 198, 480
DinI GGCGCC 1 cut(s) 427
DpnI GATC 4 cut(s) 83, 182, 329, 389
DpnII GATC 4 cut(s) 81, 180, 327, 387
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 2 cut(s) 3, 435
EagI CGGCCG 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
EclXI CGGCCG 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 191
Eco52I CGGCCG 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 427
EheI GGCGCC 1 cut(s) 427
FaeI CATG 4 cut(s) 28, 106, 307, 406
FaiI YATR 6 cut(s) 26, 104, 264, 305, 404, 470
FalI AAGNNNNNCTT 3 cut(s) 37, 170, 202
FaqI GGGAC 1 cut(s) 65
FatI CATG 4 cut(s) 24, 102, 303, 402
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 127, 203, 424
FokI GGATG 1 cut(s) 509
Fsp4HI GCNGC 3 cut(s) 127, 203, 424
FspBI CTAG 1 cut(s) 450
GlaI GCGC 4 cut(s) 119, 261, 427, 487
GluI GCNGC 3 cut(s) 127, 203, 424
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 3 cut(s) 5, 17, 437
HapII CCGG 3 cut(s) 123, 172, 206
HhaI GCGC 4 cut(s) 120, 262, 428, 488
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 4 cut(s) 28, 106, 307, 406
Hin6I GCGC 4 cut(s) 118, 260, 426, 486
HinP1I GCGC 4 cut(s) 118, 260, 426, 486
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 6 cut(s) 196, 227, 250, 274, 296, 340
HpaII CCGG 3 cut(s) 123, 172, 206
HphI GGTGA 3 cut(s) 127, 259, 382
Hpy166II GTNNAC 3 cut(s) 34, 295, 370
Hpy188I TCNGA 5 cut(s) 191, 232, 279, 301, 339
Hpy188III TCNNGA 1 cut(s) 254
Hpy8I GTNNAC 3 cut(s) 34, 295, 370
Hpy99I CGWCG 2 cut(s) 65, 247
HpyAV CCTTC 1 cut(s) 487
HpyCH4IV ACGT 2 cut(s) 332, 398
HpyCH4V TGCA 3 cut(s) 271, 361, 381
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 268, 434, 485
HpyF3I CTNAG 3 cut(s) 153, 198, 480
HpySE526I ACGT 2 cut(s) 332, 398
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 4 cut(s) 28, 106, 307, 406
HspAI GCGC 4 cut(s) 118, 260, 426, 486
KasI GGCGCC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 4 cut(s) 81, 180, 327, 387
LguI GCTCTTC 1 cut(s) 413
LpnPI CCDG 7 cut(s) 34, 100, 136, 148, 185, 219, 386
Lsp1109I GCAGC 1 cut(s) 138
MaeI CTAG 1 cut(s) 450
MaeII ACGT 2 cut(s) 332, 398
MaeIII GTNAC 1 cut(s) 143
MalI GATC 4 cut(s) 83, 182, 329, 389
MbiI CCGCTC 1 cut(s) 423
MboI GATC 4 cut(s) 81, 180, 327, 387
MboII GAAGA 6 cut(s) 91, 178, 187, 190, 427, 430
MluCI AATT 5 cut(s) 349, 362, 382, 441, 519
Mly113I GGCGCC 1 cut(s) 426
MlyI GAGTC 3 cut(s) 190, 221, 290
MseI TTAA 1 cut(s) 522
MspI CCGG 3 cut(s) 123, 172, 206
MwoI GCNNNNNNNGC 4 cut(s) 126, 268, 434, 485
NarI GGCGCC 1 cut(s) 426
NdeII GATC 4 cut(s) 81, 180, 327, 387
NlaIII CATG 4 cut(s) 28, 106, 307, 406
NlaIV GGNNCC 1 cut(s) 427
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 254
PciSI GCTCTTC 1 cut(s) 413
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 3 cut(s) 250, 274, 340
PkrI GCNGC 3 cut(s) 128, 204, 425
PleI GAGTC 3 cut(s) 190, 221, 290
PluTI GGCGCC 1 cut(s) 429
PpsI GAGTC 3 cut(s) 190, 221, 290
Ppu21I YACGTR 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 2 cut(s) 16, 191
RsaI GTAC 2 cut(s) 317, 371
RsaNI GTAC 2 cut(s) 316, 370
Rsr2I CGGWCCG 1 cut(s) 191
RsrII CGGWCCG 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 3 cut(s) 127, 203, 424
Sau3AI GATC 4 cut(s) 81, 180, 327, 387
Sau96I GGNCC 2 cut(s) 16, 191
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 3 cut(s) 190, 221, 290
SetI ASST 7 cut(s) 131, 167, 269, 335, 375, 401, 504
SfoI GGCGCC 1 cut(s) 427
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 449
Sse9I AATT 5 cut(s) 349, 362, 382, 441, 519
SsiI CCGC 3 cut(s) 19, 203, 423
SspDI GGCGCC 1 cut(s) 425
SspMI CTAG 1 cut(s) 450
TaiI ACGT 2 cut(s) 335, 401
TaqI TCGA 4 cut(s) 63, 225, 255, 312
TaqII GACCGA 1 cut(s) 208
TasI AATT 5 cut(s) 349, 362, 382, 441, 519
TatI WGTACW 1 cut(s) 315
TauI GCSGC 2 cut(s) 205, 426
TfiI GAWTC 3 cut(s) 250, 274, 340
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 1 cut(s) 126
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 3 cut(s) 13, 119, 391
TspGWI ACGGA 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 191
XceI RCATGY 1 cut(s) 307
XhoI CTCGAG 1 cut(s) 254
XspI CTAG 1 cut(s) 450
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.