pycom11g18860

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007495
Physical Location & Seq
Reverse (-)
21062542 .. 21063984
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g18860.1

Sequence Viewer

Length: 1443 bp
ATGAGTCAAACACAAAACCAGAATTACTTTAAAAACCCCACGCCCATTTCGTGGTACTTTCTCAATTCCCTCTCTATAAAATCTCAACCAATTCCTCACAAAACACCATCAATCTCAAGTTTGATAGCAGAGTGGCAAACCCTACCAATTTTGCAGCATTTCTCGATGGCTACGCTTCAATCTTCTTTTTGTTCTTCTTCTCGTTTTTCTTCAGCTACGTTTAGAAACACAAGAGCCGCTCTTCACACACCAAAATTCCAAAAATCATCCTCTCTCTCAGTTCCTAGACAGCTCCTTGTGAATGAATTAAATCTAGCAGCTACTTCAACTGATTTAGAACACAACCCGAAATTCGGAAACAAGAAAAAATCAAATCGTAACGAACCAAATTCGGATTCGAATTTGGTGCAAGAGCTGTACGCAGTAATGGAGATTGTGTCTGACAGAGCAGAAATGCATAAGAACATATGTGCTCAGAGGGACAACTGGAACGCCCTACTTTTGAACTCCGTGAACGGAATGACAGCCACGGCGGCTATCATGGCCGGATTCGCATCAGCGGCAGTATCCGGAGCGTCCATTCTGCCCCTCAAGCCCTCTGCAGGCCTCCTCTACATTTCCGTCACCGGGATTCTGCTGGTGATGAACAAAATCCAGCCTTCCCAGCTGGCGGAAGAACAGAGAAACGCTTCGAGGCTGTTCAAACGGCTTCATCAAGAGATCAAGACGACGCTGTTTCTGAAAACTCCGACTTCAAACGATGTGAAACTTGCAATGGAGAAGGTGCTGGCATTGGACAAGGCCTACCCGCTTCCTCTTTTGGGAACCATGATCGAGAAGTTCCCTTCCCACGTGGAGCCCGCTGAGTGGTGGCCGGAGGAGCATGAGACTGAGCATGAGAAGGCGGGCGAGGAGGAGGCAGAGAGGAACGGGTGGGATGAGAAGTTGGAGAAGGAAATGAAGAGCTTGTTGGGTGTGGTGAGAGGAAAAGATGGTGCGGAATACGTGAGGCTCAGCAATGTGGTGCTGAAGGTGAACAAGATTCTCGCAATGTGTGGACCAATGCTGACCGGGTTTGCGGCGGCTGGGGCGGGGGTTGTAGGGTGTGGTGGGTTTGTTGGGTGGTGGGGTGCGGTGCTAGCTGTGGTTTGTGGGGGCTTGGGTTTGGTGGTGAATAGTTTGGAGCACGGCGGGCAGGTGGGGATGGTGTTTGAGATGTATAGGAGTTCTGCTGGGTTTTTTAAGCTGATGGAGGAGACGATTGAGTCGGCGTTGAAGGAAGGGGAGGTTGGGGGGGAGAGGGAGAATGGCGAGGTGTTCGAAGTGAAAGTGGCGCTTCAGCTCGGAAGGAGCATTTCGGAGCTCAGAGATCTGGCGAGTTCTGAGTCTTTGGCTTCCAGGTTGAAGCAGGTCAAAGAGGAGTTTGCAAGCAAGCTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

481

Amino Acids

52.74

Weight (kDa)

6.82

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 139 - 456 1e-141 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1186
AasI GACNNNNNNGTC 1 cut(s) 1264
Acc36I ACCTGC 2 cut(s) 1186, 1399
AccB7I CCANNNNNTGG 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 239
AccIII TCCGGA 1 cut(s) 569
AcoI YGGCCR 2 cut(s) 543, 872
AcsI RAATTY 4 cut(s) 254, 350, 388, 400
AcuI CTGAAG 3 cut(s) 195, 1049, 1322
AcvI CACGTG 1 cut(s) 853
AfaI GTAC 2 cut(s) 56, 419
AfiI CCNNNNNNNGG 7 cut(s) 51, 353, 602, 627, 670, 821, 867
AgsI TTSAA 7 cut(s) 179, 327, 505, 703, 756, 1276, 1405
AjnI CCWGG 1 cut(s) 1397
AjuI GAANNNNNNNTTGG 6 cut(s) 379, 411, 953, 985, 1272, 1304
Alw21I GWGCWC 3 cut(s) 475, 1188, 1365
Alw26I GTCTC 2 cut(s) 881, 1250
Aor13HI TCCGGA 1 cut(s) 569
AoxI GGCC 4 cut(s) 543, 604, 801, 872
ApeKI GCWGC 2 cut(s) 154, 317
ApoI RAATTY 4 cut(s) 254, 350, 388, 400
Asp700I GAANNNNTTC 1 cut(s) 688
AspLEI GCGC 1 cut(s) 1336
AspS9I GGNCC 1 cut(s) 1058
AsuC2I CCSGG 2 cut(s) 628, 1072
AsuHPI GGTGA 5 cut(s) 616, 652, 991, 1045, 1183
AsuII TTCGAA 2 cut(s) 398, 1320
AsuNHI GCTAGC 1 cut(s) 1138
AvaII GGWCC 1 cut(s) 1058
BanII GRGCYC 2 cut(s) 861, 1365
BbrPI CACGTG 1 cut(s) 853
Bbv12I GWGCWC 3 cut(s) 475, 1188, 1365
BbvI GCAGC 2 cut(s) 166, 329
BccI CCATC 5 cut(s) 115, 160, 986, 1198, 1243
BceAI ACGGC 3 cut(s) 546, 722, 1204
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BciT130I CCWGG 1 cut(s) 1399
BciVI GTATCC 1 cut(s) 577
BcnI CCSGG 2 cut(s) 628, 1072
BcoDI GTCTC 2 cut(s) 881, 1250
BfaI CTAG 3 cut(s) 285, 314, 1139
BfmI CTRYAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 1337
BfuAI ACCTGC 2 cut(s) 1186, 1399
BfuI GTATCC 1 cut(s) 577
BglI GCCNNNNNGGC 1 cut(s) 533
BglII AGATCT 1 cut(s) 1369
BisI GCNGC 7 cut(s) 155, 237, 318, 534, 561, 1080, 1083
BlpI GCTNAGC 1 cut(s) 1013
BlsI GCNGC 7 cut(s) 156, 238, 319, 535, 562, 1081, 1084
Bme1390I CCNGG 3 cut(s) 628, 1072, 1399
Bme18I GGWCC 1 cut(s) 1058
BmgT120I GGNCC 1 cut(s) 1058
BmiI GGNNCC 2 cut(s) 826, 858
BmrFI CCNGG 3 cut(s) 628, 1072, 1399
BmsI GCATC 1 cut(s) 563
BmtI GCTAGC 1 cut(s) 1142
Bpu1102I GCTNAGC 1 cut(s) 1013
Bpu14I TTCGAA 2 cut(s) 398, 1320
BpuEI CTTGAG 2 cut(s) 100, 575
BpuMI CCSGG 2 cut(s) 628, 1072
BsaAI YACGTR 2 cut(s) 853, 1006
BsaBI GATNNNNATC 1 cut(s) 553
BsaJI CCNNGG 1 cut(s) 528
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 7 cut(s) 51, 353, 602, 627, 670, 821, 867
Bse1I ACTGG 1 cut(s) 491
Bse3DI GCAATG 3 cut(s) 780, 1024, 1056
Bse8I GATNNNNATC 1 cut(s) 553
BseAI TCCGGA 1 cut(s) 569
BseBI CCWGG 1 cut(s) 1399
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 3 cut(s) 266, 943, 1209
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 7 cut(s) 51, 353, 602, 627, 670, 821, 867
BseMI GCAATG 3 cut(s) 780, 1024, 1056
BseMII CTCAG 7 cut(s) 291, 488, 855, 882, 1027, 1374, 1378
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 6 cut(s) 599, 893, 926, 929, 1268, 1433
BseXI GCAGC 2 cut(s) 166, 329
BseYI CCCAGC 3 cut(s) 663, 1085, 1232
BshFI GGCC 4 cut(s) 545, 606, 803, 874
BsiHKAI GWGCWC 3 cut(s) 475, 1188, 1365
BsiSI CCGG 5 cut(s) 546, 570, 627, 875, 1071
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 7 cut(s) 51, 353, 602, 627, 670, 821, 867
BsmAI GTCTC 2 cut(s) 881, 1250
BsmBI CGTCTC 1 cut(s) 1250
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 4 cut(s) 545, 606, 803, 874
Bsp119I TTCGAA 2 cut(s) 398, 1320
Bsp1286I GDGCHC 4 cut(s) 475, 861, 1188, 1365
Bsp13I TCCGGA 1 cut(s) 569
Bsp143I GATC 3 cut(s) 720, 831, 1369
Bsp1720I GCTNAGC 1 cut(s) 1013
BspANI GGCC 4 cut(s) 545, 606, 803, 874
BspCNI CTCAG 7 cut(s) 290, 487, 856, 883, 1026, 1375, 1377
BspEI TCCGGA 1 cut(s) 569
BspLI GGNNCC 2 cut(s) 826, 858
BspMAI CTGCAG 1 cut(s) 604
BspMI ACCTGC 2 cut(s) 1186, 1399
BspOI GCTAGC 1 cut(s) 1142
BspQI GCTCTTC 2 cut(s) 246, 956
BspT104I TTCGAA 2 cut(s) 398, 1320
BsrBI CCGCTC 1 cut(s) 239
BsrDI GCAATG 3 cut(s) 780, 1024, 1056
BsrI ACTGG 1 cut(s) 491
BssECI CCNNGG 1 cut(s) 528
BssMI GATC 3 cut(s) 720, 831, 1369
Bst2UI CCWGG 1 cut(s) 1399
Bst6I CTCTTC 2 cut(s) 246, 956
BstBAI YACGTR 2 cut(s) 853, 1006
BstBI TTCGAA 2 cut(s) 398, 1320
BstC8I GCNNGC 9 cut(s) 604, 669, 789, 861, 907, 1140, 1193, 1429, 1433
BstDEI CTNAG 7 cut(s) 277, 474, 864, 891, 1013, 1364, 1383
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 3 cut(s) 266, 943, 1209
BstH2I RGCGCY 1 cut(s) 1337
BstHHI GCGC 1 cut(s) 1336
BstKTI GATC 3 cut(s) 723, 834, 1372
BstMAI GTCTC 2 cut(s) 881, 1250
BstMBI GATC 3 cut(s) 720, 831, 1369
BstNI CCWGG 1 cut(s) 1399
BstSCI CCNGG 3 cut(s) 626, 1070, 1397
BstSFI CTRYAG 1 cut(s) 600
BstV1I GCAGC 2 cut(s) 166, 329
BstX2I RGATCY 1 cut(s) 1369
BstYI RGATCY 1 cut(s) 1369
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 4 cut(s) 545, 606, 803, 874
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 266, 943, 1209
BveI ACCTGC 2 cut(s) 1186, 1399
Cac8I GCNNGC 9 cut(s) 604, 669, 789, 861, 907, 1140, 1193, 1429, 1433
CfoI GCGC 1 cut(s) 1336
Cfr13I GGNCC 1 cut(s) 1058
CseI GACGC 2 cut(s) 564, 739
Csp6I GTAC 2 cut(s) 55, 418
CviAII CATG 4 cut(s) 541, 829, 884, 896
CviQI GTAC 2 cut(s) 55, 418
DdeI CTNAG 7 cut(s) 277, 474, 864, 891, 1013, 1364, 1383
DpnI GATC 3 cut(s) 722, 833, 1371
DpnII GATC 3 cut(s) 720, 831, 1369
DraI TTTAAA 1 cut(s) 31
DrdI GACNNNNNNGTC 1 cut(s) 1264
DseDI GACNNNNNNGTC 1 cut(s) 1264
EaeI YGGCCR 2 cut(s) 543, 872
Eam1104I CTCTTC 2 cut(s) 246, 956
EarI CTCTTC 2 cut(s) 246, 956
EciI GGCGGA 1 cut(s) 686
Ecl136II GAGCTC 1 cut(s) 1363
Eco147I AGGCCT 2 cut(s) 606, 803
Eco24I GRGCYC 2 cut(s) 861, 1365
Eco47I GGWCC 1 cut(s) 1058
Eco53kI GAGCTC 1 cut(s) 1363
Eco57I CTGAAG 3 cut(s) 195, 1049, 1322
Eco72I CACGTG 1 cut(s) 853
EcoICRI GAGCTC 1 cut(s) 1363
EcoRII CCWGG 1 cut(s) 1397
EcoT22I ATGCAT 1 cut(s) 459
EcoT38I GRGCYC 2 cut(s) 861, 1365
Esp3I CGTCTC 1 cut(s) 1250
FaeI CATG 4 cut(s) 544, 832, 887, 899
FaiI YATR 9 cut(s) 77, 459, 467, 469, 542, 830, 885, 897, 1221
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FaqI GGGAC 1 cut(s) 494
FatI CATG 4 cut(s) 540, 828, 883, 895
FauI CCCGC 5 cut(s) 816, 868, 898, 1084, 1184
FauNDI CATATG 1 cut(s) 467
Fnu4HI GCNGC 7 cut(s) 155, 237, 318, 534, 561, 1080, 1083
FokI GGATG 3 cut(s) 253, 950, 1216
FriOI GRGCYC 2 cut(s) 861, 1365
Fsp4HI GCNGC 7 cut(s) 155, 237, 318, 534, 561, 1080, 1083
FspBI CTAG 3 cut(s) 285, 314, 1139
GlaI GCGC 1 cut(s) 1335
GluI GCNGC 7 cut(s) 155, 237, 318, 534, 561, 1080, 1083
GsaI CCCAGC 3 cut(s) 667, 1089, 1236
HaeII RGCGCY 1 cut(s) 1337
HaeIII GGCC 4 cut(s) 545, 606, 803, 874
HapII CCGG 5 cut(s) 546, 570, 627, 875, 1071
HgaI GACGC 2 cut(s) 564, 739
HhaI GCGC 1 cut(s) 1336
Hin1II CATG 4 cut(s) 544, 832, 887, 899
Hin6I GCGC 1 cut(s) 1334
HinP1I GCGC 1 cut(s) 1334
HindIII AAGCTT 1 cut(s) 1433
HinfI GANTC 7 cut(s) 4, 395, 549, 631, 1042, 1265, 1385
HpaII CCGG 5 cut(s) 546, 570, 627, 875, 1071
HphI GGTGA 5 cut(s) 616, 652, 991, 1045, 1183
Hpy166II GTNNAC 3 cut(s) 514, 1036, 1058
Hpy188III TCNNGA 5 cut(s) 163, 570, 716, 724, 835
Hpy8I GTNNAC 3 cut(s) 514, 1036, 1058
Hpy99I CGWCG 1 cut(s) 733
HpyAV CCTTC 9 cut(s) 669, 775, 855, 895, 946, 1024, 1270, 1274, 1341
HpyCH4IV ACGT 3 cut(s) 218, 852, 1005
HpyCH4V TGCA 6 cut(s) 154, 409, 457, 602, 773, 1427
HpyF3I CTNAG 7 cut(s) 277, 474, 864, 891, 1013, 1364, 1383
HpySE526I ACGT 3 cut(s) 218, 852, 1005
Hsp92II CATG 4 cut(s) 544, 832, 887, 899
HspAI GCGC 1 cut(s) 1334
Kpn2I TCCGGA 1 cut(s) 569
Kzo9I GATC 3 cut(s) 720, 831, 1369
LguI GCTCTTC 2 cut(s) 246, 956
LmnI GCTCC 7 cut(s) 297, 572, 856, 880, 1183, 1350, 1360
Lsp1109I GCAGC 2 cut(s) 166, 329
LweI GCATC 1 cut(s) 563
MaeI CTAG 3 cut(s) 285, 314, 1139
MaeII ACGT 3 cut(s) 218, 852, 1005
MaeIII GTNAC 2 cut(s) 377, 622
MalI GATC 3 cut(s) 722, 833, 1371
MbiI CCGCTC 1 cut(s) 239
MboI GATC 3 cut(s) 720, 831, 1369
MboII GAAGA 7 cut(s) 174, 186, 189, 201, 233, 686, 973
MflI RGATCY 1 cut(s) 1369
MhlI GDGCHC 4 cut(s) 475, 861, 1188, 1365
MluCI AATT 9 cut(s) 22, 64, 90, 147, 254, 305, 350, 388, 400
MlyI GAGTC 3 cut(s) 13, 1274, 1394
MmeI TCCRAC 2 cut(s) 773, 927
Mph1103I ATGCAT 1 cut(s) 459
MroI TCCGGA 1 cut(s) 569
MroXI GAANNNNTTC 1 cut(s) 688
MseI TTAA 3 cut(s) 30, 308, 1242
MspA1I CMGCKG 3 cut(s) 560, 667, 863
MspI CCGG 5 cut(s) 546, 570, 627, 875, 1071
MspR9I CCNGG 3 cut(s) 628, 1072, 1399
MvaI CCWGG 1 cut(s) 1399
NciI CCSGG 2 cut(s) 628, 1072
NdeI CATATG 1 cut(s) 467
NdeII GATC 3 cut(s) 720, 831, 1369
NheI GCTAGC 1 cut(s) 1138
NlaIII CATG 4 cut(s) 544, 832, 887, 899
NlaIV GGNNCC 2 cut(s) 826, 858
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 459
NspV TTCGAA 2 cut(s) 398, 1320
PaqCI CACCTGC 1 cut(s) 1186
PceI AGGCCT 2 cut(s) 606, 803
PciSI GCTCTTC 2 cut(s) 246, 956
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 688
PfeI GAWTC 4 cut(s) 395, 549, 631, 1042
PflMI CCANNNNNTGG 1 cut(s) 51
PkrI GCNGC 7 cut(s) 156, 238, 319, 535, 562, 1081, 1084
PleI GAGTC 3 cut(s) 12, 1273, 1393
PmaCI CACGTG 1 cut(s) 853
PmlI CACGTG 1 cut(s) 853
PpsI GAGTC 3 cut(s) 12, 1273, 1393
Ppu21I YACGTR 2 cut(s) 853, 1006
Psp124BI GAGCTC 1 cut(s) 1365
Psp6I CCWGG 1 cut(s) 1397
PspCI CACGTG 1 cut(s) 853
PspFI CCCAGC 3 cut(s) 663, 1085, 1232
PspGI CCWGG 1 cut(s) 1397
PspN4I GGNNCC 2 cut(s) 826, 858
PspPI GGNCC 1 cut(s) 1058
PstI CTGCAG 1 cut(s) 604
PsuI RGATCY 1 cut(s) 1369
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 2 cut(s) 56, 419
RsaNI GTAC 2 cut(s) 55, 418
SacI GAGCTC 1 cut(s) 1365
SapI GCTCTTC 2 cut(s) 246, 956
SaqAI TTAA 3 cut(s) 30, 308, 1242
SatI GCNGC 7 cut(s) 155, 237, 318, 534, 561, 1080, 1083
Sau3AI GATC 3 cut(s) 720, 831, 1369
Sau96I GGNCC 1 cut(s) 1058
SchI GAGTC 3 cut(s) 13, 1274, 1394
ScrFI CCNGG 3 cut(s) 628, 1072, 1399
SduI GDGCHC 4 cut(s) 475, 861, 1188, 1365
SfaNI GCATC 1 cut(s) 563
SfcI CTRYAG 1 cut(s) 600
SfuI TTCGAA 2 cut(s) 398, 1320
SinI GGWCC 1 cut(s) 1058
SmlI CTYRAG 2 cut(s) 115, 590
SmoI CTYRAG 2 cut(s) 115, 590
Sse9I AATT 9 cut(s) 22, 64, 90, 147, 254, 305, 350, 388, 400
SseBI AGGCCT 2 cut(s) 606, 803
SspMI CTAG 3 cut(s) 285, 314, 1139
SstI GAGCTC 1 cut(s) 1365
StuI AGGCCT 2 cut(s) 606, 803
StyD4I CCNGG 3 cut(s) 626, 1070, 1397
TaiI ACGT 3 cut(s) 221, 855, 1008
TaqI TCGA 5 cut(s) 164, 398, 692, 834, 1320
TasI AATT 9 cut(s) 22, 64, 90, 147, 254, 305, 350, 388, 400
TauI GCSGC 5 cut(s) 239, 536, 563, 1082, 1085
TfiI GAWTC 4 cut(s) 395, 549, 631, 1042
Tru1I TTAA 3 cut(s) 30, 308, 1242
Tru9I TTAA 3 cut(s) 30, 308, 1242
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 2 cut(s) 154, 317
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 4 cut(s) 318, 659, 701, 974
TspGWI ACGGA 3 cut(s) 499, 531, 610
Van91I CCANNNNNTGG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 1058
XapI RAATTY 4 cut(s) 254, 350, 388, 400
XmnI GAANNNNTTC 1 cut(s) 688
XspI CTAG 3 cut(s) 285, 314, 1139
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.