Rh5BG277500

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007495
Physical Location & Seq
Forward (+)
34986059 .. 34987521
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG277500.1

Sequence Viewer

Length: 1290 bp
ATGGCCACTATTCAAGCTTCTTTTTGTTCTGCTTCATTTTCTTCAGCTTCCTCTAGAAAAACCAAAGTTACTTTCCACTCCCAAAACTTTGAATCATCGCATCTTTCAGTTCCCAGGCTCCTGGTTAATGAACTGAATCAAAGAAATGGGTCACCCAACCTAACAGCCACTTCATCTGAAATACTAGAAGTGAAACCATTATTTGACAACAGAAAAAGATCAAATCTCGGTGACTCAGTTTCAGATTCAAATTTTGTCCAAGAGCTCTATGCAATAATGGAAATTGTGGCAGATAGAATAGAAATGCACAACAACATAAAGGCGCAGAGGGACAACTGGAATGTTCTGCTTCTCAACTCTGTGAATGGGATGACAGCCACAGCTGCAATCATGGCGGGACTTGCATCTGTCTCTGGATCTGGGCCGTCCATTTTGTCTCTCAAGATCTCATCAAGTCTCCTGTACATGGCTGTGACTGGGATTGTTCTAGTGATGAACAAGATCCAACCTTCACAGCTAGCAGAGGAACAAAGAAATGCTTCAAGGCTGTTCAAGAAGCTTCATGAAGAAATCAAAACCACTTTGGCTCTCAAAAACCCAGTTACTTCTTATGATGTGAAAGTCACAATGGAAAAGGTTTTGGCTTTGGATAAGGCATACCCACTTCCCTTACTTGGAACCATGATTGAGAAGTTTCCTCAAATTGTGAAGCCAGCTGTGTGGTGGCCTGAGGAAGAACCCAAGTGCCATGATCATAACATGAGTGACAAGTTTGAGAGGAATGGGTGGAATGAGAATCTTGAAGAGGAACTGAAGGAGGTTTTGTGCGTAATGAAGGGAAGGGACTCCGCAGAATATGCAAGGCTAAGCAACATGGTGTTGAAGGTCAACAAGGTCTTGGCTTTCTGTGGTCCTTTGCTTACTGGGTCTGCTGCAATTGGGTCAGGTTTTATTGGATTGCCTGGTTTTGGGTCATGGGGTGCTGTTTTGGGTGTGGTTTTGGGGGCTTTGGGCACTGTGGTTAACAGTTTGGAGCATGGAGGGCAAGTTGGGATGGTGTTTGAGATGTATAGGAGCTCTGCTGGGTTCTTCAAGCTCATGGAGGAGACCATTGAGTCAACTTTGAAGGAAAGAGAGGTTGGAGATAGAGAAAATGGGGAGCTCTTTGAAGTGAAAGTGGCTCTTCAGCTTGGGAGGAGTTTGTCTGAGCTCAGAGACCTCGCAAGTGCTTCATCTTTGTCTTCGAGAATTCAACAGGCCAAAGAAGAATTTGCAAGCAAGCTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chloroplast_duf

Protein Analysis

429

Amino Acids

47.12

Weight (kDa)

6.24

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroplast_duf PF14476 89 - 405 2.5e-147 Petal formation-expressed
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1114
AciI CCGC 2 cut(s) 395, 849
AclWI GGATC 2 cut(s) 424, 496
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 250, 1248, 1268
AcuI CTGAAG 3 cut(s) 27, 833, 1169
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 3 cut(s) 466, 674, 968
AjnI CCWGG 3 cut(s) 113, 120, 961
AjuI GAANNNNNNNTTGG 4 cut(s) 56, 88, 1122, 1154
Alw21I GWGCWC 4 cut(s) 267, 1079, 1164, 1212
Alw26I GTCTC 5 cut(s) 415, 441, 461, 1100, 1209
AlwI GGATC 2 cut(s) 424, 496
AoxI GGCC 4 cut(s) 3, 422, 725, 1257
ApeKI GCWGC 2 cut(s) 383, 932
ApoI RAATTY 3 cut(s) 250, 1248, 1268
Asp700I GAANNNNTTC 1 cut(s) 538
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 2 cut(s) 422, 911
AsuHPI GGTGA 2 cut(s) 144, 242
AsuNHI GCTAGC 1 cut(s) 517
AvaII GGWCC 1 cut(s) 911
AxyI CCTNAGG 1 cut(s) 729
BaeGI GKGCMC 1 cut(s) 1016
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 4 cut(s) 267, 1079, 1164, 1212
BbsI GAAGAC 1 cut(s) 1233
Bbv12I GWGCWC 4 cut(s) 267, 1079, 1164, 1212
BbvI GCAGC 2 cut(s) 370, 919
BccI CCATC 1 cut(s) 1048
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 3 cut(s) 115, 122, 963
BclI TGATCA 1 cut(s) 751
BcoDI GTCTC 5 cut(s) 415, 441, 461, 1100, 1209
BfaI CTAG 4 cut(s) 54, 185, 488, 518
BglII AGATCT 1 cut(s) 444
BisI GCNGC 2 cut(s) 384, 933
BlpI GCTNAGC 1 cut(s) 866
BlsI GCNGC 2 cut(s) 385, 934
Bme1390I CCNGG 3 cut(s) 115, 122, 963
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 2 cut(s) 422, 911
BmiI GGNNCC 2 cut(s) 119, 679
BmrFI CCNGG 3 cut(s) 115, 122, 963
BmrI ACTGGG 3 cut(s) 486, 593, 933
BmsI GCATC 2 cut(s) 109, 413
BmtI GCTAGC 1 cut(s) 521
BmuI ACTGGG 3 cut(s) 486, 593, 933
BpiI GAAGAC 1 cut(s) 1233
Bpu1102I GCTNAGC 1 cut(s) 866
BpuEI CTTGAG 1 cut(s) 425
BsaI GGTCTC 2 cut(s) 1100, 1209
BsaJI CCNNGG 1 cut(s) 113
BsaXI ACNNNNNCTCC 4 cut(s) 809, 839, 1032, 1062
Bsc4I CCNNNNNNNGG 3 cut(s) 466, 674, 968
Bse1I ACTGG 4 cut(s) 341, 481, 599, 928
Bse21I CCTNAGG 1 cut(s) 729
BseBI CCWGG 3 cut(s) 115, 122, 963
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 2 cut(s) 375, 1059
BseLI CCNNNNNNNGG 3 cut(s) 466, 674, 968
BseMII CTCAG 4 cut(s) 249, 720, 1197, 1225
BseNI ACTGG 4 cut(s) 341, 481, 599, 928
BseRI GAGGAG 2 cut(s) 1118, 1210
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 2 cut(s) 370, 919
BseYI CCCAGC 1 cut(s) 1082
BshFI GGCC 4 cut(s) 5, 424, 727, 1259
BsiHKAI GWGCWC 4 cut(s) 267, 1079, 1164, 1212
BslFI GGGAC 3 cut(s) 344, 411, 857
BslI CCNNNNNNNGG 3 cut(s) 466, 674, 968
BsmAI GTCTC 5 cut(s) 415, 441, 461, 1100, 1209
BsmFI GGGAC 3 cut(s) 344, 411, 857
BsnI GGCC 4 cut(s) 5, 424, 727, 1259
Bso31I GGTCTC 2 cut(s) 1100, 1209
Bsp1286I GDGCHC 5 cut(s) 267, 1016, 1079, 1164, 1212
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 5 cut(s) 218, 416, 444, 501, 751
Bsp1720I GCTNAGC 1 cut(s) 866
BspACI CCGC 2 cut(s) 395, 849
BspANI GGCC 4 cut(s) 5, 424, 727, 1259
BspCNI CTCAG 4 cut(s) 248, 721, 1198, 1224
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 119, 679
BspOI GCTAGC 1 cut(s) 521
BspPI GGATC 2 cut(s) 424, 496
BspQI GCTCTTC 1 cut(s) 1188
BspTNI GGTCTC 2 cut(s) 1100, 1209
BsrGI TGTACA 1 cut(s) 462
BsrI ACTGG 4 cut(s) 341, 481, 599, 928
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 5 cut(s) 218, 416, 444, 501, 751
Bst2UI CCWGG 3 cut(s) 115, 122, 963
Bst4CI ACNGT 2 cut(s) 1018, 1028
Bst6I CTCTTC 2 cut(s) 798, 1188
BstAPI GCANNNNNTGC 1 cut(s) 857
BstAUI TGTACA 1 cut(s) 462
BstC8I GCNNGC 4 cut(s) 519, 714, 1276, 1280
BstDEI CTNAG 5 cut(s) 235, 729, 866, 1206, 1211
BstEII GGTNACC 1 cut(s) 150
BstF5I GGATG 2 cut(s) 375, 1059
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 5 cut(s) 221, 419, 447, 504, 754
BstMAI GTCTC 5 cut(s) 415, 441, 461, 1100, 1209
BstMBI GATC 5 cut(s) 218, 416, 444, 501, 751
BstMWI GCNNNNNNNGC 5 cut(s) 383, 392, 401, 857, 1042
BstNI CCWGG 3 cut(s) 115, 122, 963
BstPI GGTNACC 1 cut(s) 150
BstSCI CCNGG 3 cut(s) 113, 120, 961
BstSLI GKGCMC 1 cut(s) 1016
BstV1I GCAGC 2 cut(s) 370, 919
BstV2I GAAGAC 1 cut(s) 1233
BstX2I RGATCY 3 cut(s) 416, 444, 501
BstXI CCANNNNNNTGG 2 cut(s) 121, 720
BstYI RGATCY 3 cut(s) 416, 444, 501
Bsu36I CCTNAGG 1 cut(s) 729
BsuRI GGCC 4 cut(s) 5, 424, 727, 1259
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 2 cut(s) 375, 1059
BtsIMutI CAGTG 1 cut(s) 1014
Cac8I GCNNGC 4 cut(s) 519, 714, 1276, 1280
CciI TCATGA 1 cut(s) 562
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 422, 911
Csp6I GTAC 1 cut(s) 463
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 5 cut(s) 235, 729, 866, 1206, 1211
DpnI GATC 5 cut(s) 220, 418, 446, 503, 753
DpnII GATC 5 cut(s) 218, 416, 444, 501, 751
DrdI GACNNNNNNGTC 1 cut(s) 1114
DseDI GACNNNNNNGTC 1 cut(s) 1114
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 798, 1188
EarI CTCTTC 2 cut(s) 798, 1188
Ecl136II GAGCTC 4 cut(s) 265, 1077, 1162, 1210
Eco24I GRGCYC 4 cut(s) 267, 1079, 1164, 1212
Eco31I GGTCTC 2 cut(s) 1100, 1209
Eco47I GGWCC 1 cut(s) 911
Eco53kI GAGCTC 4 cut(s) 265, 1077, 1162, 1210
Eco57I CTGAAG 3 cut(s) 27, 833, 1169
Eco81I CCTNAGG 1 cut(s) 729
Eco91I GGTNACC 1 cut(s) 150
EcoICRI GAGCTC 4 cut(s) 265, 1077, 1162, 1210
EcoO65I GGTNACC 1 cut(s) 150
EcoRI GAATTC 1 cut(s) 1248
EcoRII CCWGG 3 cut(s) 113, 120, 961
EcoT38I GRGCYC 4 cut(s) 267, 1079, 1164, 1212
FalI AAGNNNNNCTT 4 cut(s) 523, 555, 1167, 1199
FaqI GGGAC 3 cut(s) 344, 411, 857
FauI CCCGC 1 cut(s) 388
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 2 cut(s) 384, 933
FokI GGATG 2 cut(s) 382, 1066
FriOI GRGCYC 4 cut(s) 267, 1079, 1164, 1212
Fsp4HI GCNGC 2 cut(s) 384, 933
FspBI CTAG 4 cut(s) 54, 185, 488, 518
GlaI GCGC 1 cut(s) 324
GluI GCNGC 2 cut(s) 384, 933
GsaI CCCAGC 1 cut(s) 1086
HaeIII GGCC 4 cut(s) 5, 424, 727, 1259
HhaI GCGC 1 cut(s) 325
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 3 cut(s) 889, 1024, 1119
HindII GTYRAC 3 cut(s) 889, 1024, 1119
HindIII AAGCTT 3 cut(s) 15, 557, 1280
HinfI GANTC 7 cut(s) 92, 136, 233, 245, 796, 845, 1115
HpaI GTTAAC 1 cut(s) 1024
HphI GGTGA 2 cut(s) 144, 242
Hpy166II GTNNAC 3 cut(s) 889, 1024, 1119
Hpy188I TCNGA 4 cut(s) 178, 244, 1207, 1214
Hpy188III TCNNGA 7 cut(s) 54, 414, 442, 553, 563, 800, 1245
Hpy8I GTNNAC 3 cut(s) 889, 1024, 1119
HpyAV CCTTC 6 cut(s) 519, 808, 829, 834, 877, 1120
HpyCH4III ACNGT 2 cut(s) 1018, 1028
HpyCH4V TGCA 7 cut(s) 272, 307, 386, 404, 860, 935, 1274
HpyF10VI GCNNNNNNNGC 5 cut(s) 383, 392, 401, 857, 1042
HpyF3I CTNAG 5 cut(s) 235, 729, 866, 1206, 1211
HspAI GCGC 1 cut(s) 323
Ksp22I TGATCA 1 cut(s) 751
KspAI GTTAAC 1 cut(s) 1024
Kzo9I GATC 5 cut(s) 218, 416, 444, 501, 751
LguI GCTCTTC 1 cut(s) 1188
LmnI GCTCC 4 cut(s) 123, 1033, 1074, 1159
Lsp1109I GCAGC 2 cut(s) 370, 919
LweI GCATC 2 cut(s) 109, 413
MaeI CTAG 4 cut(s) 54, 185, 488, 518
MaeIII GTNAC 7 cut(s) 67, 150, 230, 472, 601, 622, 764
MalI GATC 5 cut(s) 220, 418, 446, 503, 753
MboI GATC 5 cut(s) 218, 416, 444, 501, 751
MboII GAAGA 8 cut(s) 33, 578, 746, 815, 1081, 1175, 1233, 1277
MfeI CAATTG 1 cut(s) 936
MflI RGATCY 3 cut(s) 416, 444, 501
MhlI GDGCHC 5 cut(s) 267, 1016, 1079, 1164, 1212
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 250, 282, 702, 936, 1248, 1268
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 227, 839, 1124
MmeI TCCRAC 2 cut(s) 529, 1120
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 126, 1023
MslI CAYNNNNRTG 1 cut(s) 470
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 383, 716
MspR9I CCNGG 3 cut(s) 115, 122, 963
MunI CAATTG 1 cut(s) 936
MvaI CCWGG 3 cut(s) 115, 122, 963
MwoI GCNNNNNNNGC 5 cut(s) 383, 392, 401, 857, 1042
NdeII GATC 5 cut(s) 218, 416, 444, 501, 751
NheI GCTAGC 1 cut(s) 517
NlaIV GGNNCC 2 cut(s) 119, 679
NmuCI GTSAC 5 cut(s) 150, 230, 472, 622, 764
PagI TCATGA 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 1188
PdmI GAANNNNTTC 1 cut(s) 538
PfeI GAWTC 4 cut(s) 92, 136, 245, 796
PkrI GCNGC 2 cut(s) 385, 934
PleI GAGTC 3 cut(s) 227, 839, 1123
PpsI GAGTC 3 cut(s) 227, 839, 1123
Psp124BI GAGCTC 4 cut(s) 267, 1079, 1164, 1212
Psp6I CCWGG 3 cut(s) 113, 120, 961
PspEI GGTNACC 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 1082
PspGI CCWGG 3 cut(s) 113, 120, 961
PspN4I GGNNCC 2 cut(s) 119, 679
PspPI GGNCC 2 cut(s) 422, 911
PsuI RGATCY 3 cut(s) 416, 444, 501
PvuII CAGCTG 2 cut(s) 383, 716
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 470
SacI GAGCTC 4 cut(s) 267, 1079, 1164, 1212
SapI GCTCTTC 1 cut(s) 1188
SaqAI TTAA 2 cut(s) 126, 1023
SatI GCNGC 2 cut(s) 384, 933
Sau3AI GATC 5 cut(s) 218, 416, 444, 501, 751
Sau96I GGNCC 2 cut(s) 422, 911
SchI GAGTC 3 cut(s) 227, 839, 1124
ScrFI CCNGG 3 cut(s) 115, 122, 963
SduI GDGCHC 5 cut(s) 267, 1016, 1079, 1164, 1212
SfaNI GCATC 2 cut(s) 109, 413
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 6 cut(s) 250, 282, 702, 936, 1248, 1268
SsiI CCGC 2 cut(s) 395, 849
SspMI CTAG 4 cut(s) 54, 185, 488, 518
SstI GAGCTC 4 cut(s) 267, 1079, 1164, 1212
StyD4I CCNGG 3 cut(s) 113, 120, 961
TaaI ACNGT 2 cut(s) 1018, 1028
TaqI TCGA 1 cut(s) 1244
TasI AATT 6 cut(s) 250, 282, 702, 936, 1248, 1268
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 4 cut(s) 92, 136, 245, 796
Tru1I TTAA 2 cut(s) 126, 1023
Tru9I TTAA 2 cut(s) 126, 1023
TscAI CASTG 1 cut(s) 1021
TseFI GTSAC 5 cut(s) 150, 230, 472, 622, 764
TseI GCWGC 2 cut(s) 383, 932
Tsp45I GTSAC 5 cut(s) 150, 230, 472, 622, 764
TspDTI ATGAA 8 cut(s) 24, 144, 162, 509, 551, 579, 848, 1221
TspRI CASTG 1 cut(s) 1021
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 3 cut(s) 250, 1248, 1268
XbaI TCTAGA 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 538
XspI CTAG 4 cut(s) 54, 185, 488, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.