MD11G1308600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016760
Physical Location & Seq
Reverse (-)
42220209 .. 42225914
5706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1308600.v1.1.491

Sequence Viewer

Length: 3495 bp
ATGGACTCGATTTTAAACGCGTCGTTAGAAGAAATCTGCTTGGAAGGCCGAAACGGTCTCGCGATATCAACCCTCTGGTCCAGACTCGAGTCCTCTCTTTCATCCTCCAATTTTTCTGATCATGGGTTCAAACAAACCTTATGGGCGGAGCTCCGGTCAGTCCCGACCCTCAAATTCCTGAACCAGAAGCAAAATCCCCGGTACTACAGCCCAGCGGATTCTTCAATCCAGTCCTTCCAAGACGCCGAGAAACTCGACCTGAAGCTCGTCGCCGATCAATGTCTCAGGGACAGCTTCCTGGGCCTCTACATTGCCCAATCCTCCGTCGAAAACATGTGTTCGTATCAACGCCGAACCCTTGAAAGAGTTGCCACCGCTAGAAGGGATGGAATTACACAAAGTCAACTTTCCAAGGAATTGGGCATTCAAGGCAAAAACTATTTCTACAAAGTGAGAAAGCTTGAATGTCAAGGGTTAATTGCGAAGCGACCTGCATTGGTGAGGAAAAAAATTCCTGGTGACGAAGGCCACTCGAGCAATAATTTGAGCGTGAGTACCAATTTGATATACTTACATCGCTATGCAAATGATTTGGGCTCTCAACAAGAAGTTGAGATCACAAAGGTAGAACAGAATACAGAGAGTTTTGGGAATGCAAAAGAAATCCCTGCAAGTGGAGATGGATTTTCGGGAAATGGGGTTAAAGATAATGTGCTTGTAAATGACTATTTATCAGCAACGAAGGCAGTCTGCGATAAGCTCAAAGAATCCAATGGCAAGGTTCTTGTTTCGGATCTCAAGAAAGAGCTTGGTTACAGCGGAACACGTTCAGGAAATAGAGCGTGGAGGAAGATTTGTTGCAGATTGGAAGCTGCTCATATTGTGAAAAAGTTTGAGGCTCAAGTAAATGAGAAGGTTGAGCAATACCTTGTTTTGCTGAAAAAGTTTTGTCCGAAGAGTCTCGAGCCAAAAAATCTTGGGCAGGTGAAAATCGTGAAGAAATATCAAACTGCTCATCTGCTTGCGGAACTTCCGATTGAGCATCAAATTCAAGAATTGATTAATGCTGCAGGATCAGAGGGTATTATTATGTCTAATATTTCCAAGAGGCTTGGAATTGGCAGGAAAGAGAGTGATAAGTGGATTGAAAGTATGTGCTCTAGGTTTGGGATGATTAAGAGGAAAGAACGAAACAAAAAAGTCAATGAATATCGAGTTTGGGAACGAGGGAAATGCAATTTCAAATCAGCCAAGGTATTCCTTAATCAATCAGAAAATGACAACACAACAACAACAACAACAACAACACCTTATGTTAGCTATGTAGATACTCTTGATAGGACAGATCAAATTCAATCAACTTTGACAGAAACGGAAACACATGATGCCGCATCAGATACACCACTTTCTGTCTTGAATCCACATCTGACTTTGACAGTGGACAGTGCCAGAAAGGAAGAAAGCATGCTCGAACAGGATAAAGGGGCAGGACTTCAATCTCAAAAAAGTGAACATGCTTCCTGGAAAAGGAATAGATCTTCTAAAGCAAGCTCAGTAGAGTTCACAGAAGTTGAAGGGGTAACTGGAAGGCTGGAAGAGCAGATGCTTGACAAATTACCTGTTACTGTCAAAACATTCATGGAAGGAAGAAATCTCTTGTTCACTTTTCCAGGTGAGCATGACGCACATTTGCATGCGTTCCAGATGGATGGTCATCAAGAAACTGACCAGGAATCTGGAACAAAAGATACTGAGGGGTGTCATTCGATCTTGAGTATGTGCGATTTTTCAAAGTTGAAGCCATCACGTCAATTCAGGTTTTCATGGACTGAAGAGGCTGATAGGCAATTGGTTATTCAATATGCAAGATGGTGCGCAACTCTTGGGGCGAAATATCATCACCAGATAAATTGGGCTTCACTTCCTGACCTTCCAGCACCTCCTAGTACCTGCAAAAGAAGAATGTCATATCTGAAGAACAGTAATGGAAAATTTAGAAAAGCTCTGATGAAGCTCTGTAACATGCTCAGTGAAAGATATGCTATGCTCTTGCTAAAAACCCAGAATGGGTCACTTAACATATCCGATTGCAGACAACTTTTCCAAGGTTCAGCAGAGGAAGGTTATAACAAGAATTGTTCCAACATCAGTGACCGTAATCAAAGGACAGGTTTTCGGGAAAAACTGTGGGATGATTTTGATGATAAGAATATTAGAAAAAGCCTTGATGAGATACTTCACCACAGGATGACTAAAATGGATGCCACCAAAAGAGATGGATCTACGTGTGAGGCAGGGTCAGACCATAGTACAAATTCTGAAGAATATGACCCGCAGGGATCTGAATTGATTGTACCATCAGTTCCGAGCGATGAAATTCAGAATGATAGCAGAGGAATTTCTGCACAACGATGTGTTCAACACCTTCATCAGAATTTCTTTAAACTTTTGCATGAAGGAGTTGTTAGTGCACCAATCTACAAATCTTTGGCTGTAGAGCTATTTAAACTTGTCTTCTTGAGTACCATAACAGCACGAGGAGAGCAAAATCTGCCTGCAGAAATCCAACAGCGTTACTCAGAGCATGATCTTTTAGCAGCTCTCGACTACCTAAGAGATAACAAAATTGTGGTTGGGGGTACTGACAGTCAACCTCTATCCCTCTCACCTCAGTTTTTCTCTAACATTTTTAGGTCACCGTTTCCTACTGATTCTGGAAAGAGAGCTGCCAAATTGGCTCGTTGGCTCCGTGAAAGAGAGAAAGATCTTACAGAAGGGGGGATTCATCTGTCTACAGATTTAAAATGTGGGGACTTTTTTCATTTGTTTGCTCTAGTTTCTTCAGGCCAACTGTCGATTTCATCACACATTCCAGATGGAGTGGGAGAGGCTGAAGATTTGAGAAGTTCAAAACGGAAAACTGGAAGCGCTGAATCTTTGAACTCTAAAAGACTAAAATACTCAAGTGATAATCTTTCCCGCCGAGAAAAAGGTTACCCAGGAATCATGGTGTCTGTATATCTGACATCTTCTACGTCGAAGTCTGTAAATCTGTTGAAAGATGACGGTGCTTCTAGTGGCAAAAAACACGTCAGTGGAAATAATCATGTAGAACAAACTGGCAGTGTGCCTGAAGGTTGTATGGAAGACAAAATGTGTGCGCCAACACTGCCGTGGATGAATGGTGAACAGAAAATTAACAAGATTTTCGACAAGGGACTCAAACGGCGCCTTCTTGGGATTGTGATGCAAAATCCAGGGATGTTGGAGGATGAAATTATCCGGAAGATGGATGTACTGAATCCGCAGAGTTGTCGAAAATTGATGGAGTTGATGGTTCTGGACAAGCATCTTCATGTGCGGAAGTTGCATCAAACTGTTTACAGCGGTCCCCCGCCAATTCTAAGGACCCTCATCGGAAGCAGCTCCGCCCCGCCAAAAGAGGTGGTTCGTGAGCATTTCTTCGCAAATTCCGTGAGTGCAGGTATGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1165

Amino Acids

130.95

Weight (kDa)

8.73

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 103 2.7e-32 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 116 - 196 2.8e-14 B-block binding subunit of TFIIIC
DUF7599 PF24538 241 - 324 2.1e-28 Domain of unknown function (DUF7599)
DUF7646 PF24657 333 - 413 5.2e-26 Domain of unknown function (DUF7646)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23920 FvH4_2g23920
malus_domestica MD11G1308600.v1.1
prunus_persica Prupe.8G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291551
rosa_laevigata RLG00000012108
rosa_multiflora Rmu_sc0002226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175980
rosa_rugosa Rorug06G0220200
rosa_samantha Rh6AG332400 Rh6BG339300 Rh6CG345600 Rh6DG332900
rosa_wichuraiana Rw6G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2127
AarI CACCTGC 1 cut(s) 973
Acc16I TGCGCA 1 cut(s) 1876
Acc36I ACCTGC 4 cut(s) 499, 973, 1958, 3473
AccB1I GGYRCC 1 cut(s) 3228
AccI GTMKAC 1 cut(s) 2792
AccII CGCG 2 cut(s) 20, 62
AccIII TCCGGA 1 cut(s) 3282
AclWI GGATC 4 cut(s) 801, 1081, 2287, 2347
AcuI CTGAAG 7 cut(s) 281, 1851, 1994, 2340, 2826, 2913, 3153
AcyI GRCGYC 2 cut(s) 243, 3229
AfaI GTAC 8 cut(s) 203, 556, 1948, 2311, 2355, 2524, 2641, 3297
AfeI AGCGCT 1 cut(s) 2929
AfiI CCNNNNNNNGG 5 cut(s) 381, 674, 1527, 2067, 3289
AflIII ACRYGT 5 cut(s) 18, 333, 824, 2285, 3088
AjiI CACGTC 2 cut(s) 1808, 3091
AjnI CCWGG 7 cut(s) 297, 514, 1520, 1669, 1728, 2998, 3256
AleI CACNNNNGTG 2 cut(s) 3093, 3172
Alw21I GWGCWC 3 cut(s) 153, 1160, 2473
Alw26I GTCTC 3 cut(s) 62, 287, 965
Alw44I GTGCAC 1 cut(s) 2469
AlwI GGATC 4 cut(s) 801, 1081, 2287, 2347
Ama87I CYCGRG 3 cut(s) 86, 532, 962
Aor13HI TCCGGA 1 cut(s) 3282
Aor51HI AGCGCT 1 cut(s) 2929
AoxI GGCC 4 cut(s) 46, 301, 526, 2845
ApaLI GTGCAC 1 cut(s) 2469
ApeKI GCWGC 5 cut(s) 872, 1067, 2597, 2726, 3423
ArsI GACNNNNNNTTYG 2 cut(s) 3075, 3107
AseI ATTAAT 1 cut(s) 1062
Asp700I GAANNNNTTC 1 cut(s) 826
AspLEI GCGC 4 cut(s) 1877, 2930, 3163, 3231
AspS9I GGNCC 4 cut(s) 78, 301, 3389, 3408
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 9 cut(s) 511, 530, 997, 1685, 1892, 2231, 2658, 2688, 3197
AvaI CYCGRG 3 cut(s) 86, 532, 962
AvaII GGWCC 3 cut(s) 78, 3389, 3408
BaeGI GKGCMC 1 cut(s) 2473
BanI GGYRCC 1 cut(s) 3228
BanII GRGCYC 2 cut(s) 153, 599
BauI CACGAG 1 cut(s) 2535
BbsI GAAGAC 2 cut(s) 2506, 3153
Bbv12I GWGCWC 3 cut(s) 153, 1160, 2473
BbvI GCAGC 5 cut(s) 859, 1054, 2609, 2713, 3435
BceAI ACGGC 2 cut(s) 3157, 3242
BcgI CGANNNNNNTGC 4 cut(s) 1215, 1249, 3296, 3330
BciT130I CCWGG 7 cut(s) 299, 516, 1522, 1671, 1730, 3000, 3258
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 1 cut(s) 199
BcoDI GTCTC 3 cut(s) 62, 287, 965
BfaI CTAG 5 cut(s) 378, 1161, 1944, 2834, 3075
BfmI CTRYAG 6 cut(s) 205, 1068, 2493, 2556, 2793, 3491
BfoI RGCGCY 2 cut(s) 2931, 3232
BfuAI ACCTGC 4 cut(s) 499, 973, 1958, 3473
BglI GCCNNNNNGGC 1 cut(s) 2735
BglII AGATCT 2 cut(s) 1535, 2764
BisI GCNGC 6 cut(s) 873, 1068, 1389, 2598, 2727, 3424
BlsI GCNGC 6 cut(s) 874, 1069, 1390, 2599, 2728, 3425
Bme1390I CCNGG 8 cut(s) 199, 299, 516, 1522, 1671, 1730, 3000, 3258
Bme18I GGWCC 3 cut(s) 78, 3389, 3408
BmeT110I CYCGRG 3 cut(s) 86, 532, 962
BmgBI CACGTC 2 cut(s) 1808, 3091
BmgT120I GGNCC 4 cut(s) 78, 301, 3389, 3408
BmiI GGNNCC 4 cut(s) 2747, 3230, 3391, 3410
BmrFI CCNGG 8 cut(s) 199, 299, 516, 1522, 1671, 1730, 3000, 3258
BmsI GCATC 8 cut(s) 1051, 1375, 1400, 1593, 2251, 3237, 3358, 3379
BoxI GACNNNNGTC 1 cut(s) 88
BpiI GAAGAC 2 cut(s) 2506, 3153
BpuEI CTTGAG 5 cut(s) 782, 885, 1792, 2539, 2947
BpuMI CCSGG 1 cut(s) 199
BsaAI YACGTR 1 cut(s) 2286
BsaBI GATNNNNATC 1 cut(s) 1713
BsaHI GRCGYC 2 cut(s) 243, 3229
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 8 cut(s) 197, 298, 411, 1251, 2104, 2998, 3173, 3257
BsaWI WCCGGW 2 cut(s) 153, 3282
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 5 cut(s) 381, 674, 1527, 2067, 3289
Bse1I ACTGG 4 cut(s) 229, 1588, 2926, 3124
Bse3DI GCAATG 1 cut(s) 309
Bse8I GATNNNNATC 1 cut(s) 1713
BseAI TCCGGA 1 cut(s) 3282
BseBI CCWGG 7 cut(s) 299, 516, 1522, 1671, 1730, 3000, 3258
BseDI CCNNGG 8 cut(s) 197, 298, 411, 1251, 2104, 2998, 3173, 3257
BseJI GATNNNNATC 1 cut(s) 1713
BseLI CCNNNNNNNGG 5 cut(s) 381, 674, 1527, 2067, 3289
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 6 cut(s) 298, 1566, 1743, 2041, 2592, 2684
BseNI ACTGG 4 cut(s) 229, 1588, 2926, 3124
BseRI GAGGAG 1 cut(s) 2553
BseSI GKGCMC 1 cut(s) 2473
BseXI GCAGC 5 cut(s) 859, 1054, 2609, 2713, 3435
BseYI CCCAGC 1 cut(s) 211
BsgI GTGCAG 1 cut(s) 2388
Bsh1236I CGCG 2 cut(s) 20, 62
BshFI GGCC 4 cut(s) 48, 303, 528, 2847
BshNI GGYRCC 1 cut(s) 3228
BsiHKAI GWGCWC 3 cut(s) 153, 1160, 2473
BsiHKCI CYCGRG 3 cut(s) 86, 532, 962
BsiSI CCGG 3 cut(s) 154, 199, 3283
BslFI GGGAC 5 cut(s) 146, 302, 2825, 3231, 3375
BslI CCNNNNNNNGG 5 cut(s) 381, 674, 1527, 2067, 3289
BsmAI GTCTC 3 cut(s) 62, 287, 965
BsmFI GGGAC 5 cut(s) 146, 302, 2825, 3231, 3375
BsmI GAATGC 2 cut(s) 423, 658
BsnI GGCC 4 cut(s) 48, 303, 528, 2847
Bso31I GGTCTC 1 cut(s) 62
BsoBI CYCGRG 3 cut(s) 86, 532, 962
Bsp1286I GDGCHC 4 cut(s) 153, 599, 1160, 2473
Bsp13I TCCGGA 1 cut(s) 3282
Bsp68I TCGCGA 1 cut(s) 62
BspANI GGCC 4 cut(s) 48, 303, 528, 2847
BspCNI CTCAG 6 cut(s) 297, 1565, 1744, 2040, 2591, 2683
BspEI TCCGGA 1 cut(s) 3282
BspFNI CGCG 2 cut(s) 20, 62
BspLI GGNNCC 4 cut(s) 2747, 3230, 3391, 3410
BspMAI CTGCAG 2 cut(s) 1072, 2560
BspMI ACCTGC 4 cut(s) 499, 973, 1958, 3473
BspPI GGATC 4 cut(s) 801, 1081, 2287, 2347
BspQI GCTCTTC 1 cut(s) 1590
BspT107I GGYRCC 1 cut(s) 3228
BspTNI GGTCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 4 cut(s) 229, 1588, 2926, 3124
BssECI CCNNGG 8 cut(s) 197, 298, 411, 1251, 2104, 2998, 3173, 3257
BssNI GRCGYC 2 cut(s) 243, 3229
BssSI CACGAG 1 cut(s) 2535
BssT1I CCWWGG 3 cut(s) 411, 1251, 2104
Bst2BI CACGAG 1 cut(s) 2535
Bst2UI CCWGG 7 cut(s) 299, 516, 1522, 1671, 1730, 3000, 3258
Bst6I CTCTTC 3 cut(s) 950, 1590, 1827
BstACI GRCGYC 2 cut(s) 243, 3229
BstAPI GCANNNNNTGC 1 cut(s) 2551
BstBAI YACGTR 1 cut(s) 2286
BstC8I GCNNGC 5 cut(s) 1023, 1466, 1549, 1695, 2556
BstDEI CTNAG 8 cut(s) 284, 1552, 1752, 2027, 2578, 2612, 2670, 3404
BstDSI CCRYGG 1 cut(s) 3173
BstEII GGTNACC 2 cut(s) 2694, 2993
BstENI CCTNNNNNAGG 1 cut(s) 1525
BstFNI CGCG 2 cut(s) 20, 62
BstH2I RGCGCY 2 cut(s) 2931, 3232
BstHHI GCGC 4 cut(s) 1877, 2930, 3163, 3231
BstMAI GTCTC 3 cut(s) 62, 287, 965
BstNI CCWGG 7 cut(s) 299, 516, 1522, 1671, 1730, 3000, 3258
BstNSI RCATGY 5 cut(s) 337, 1468, 1517, 1697, 2026
BstPAI GACNNNNGTC 1 cut(s) 88
BstPI GGTNACC 2 cut(s) 2694, 2993
BstSCI CCNGG 8 cut(s) 197, 297, 514, 1520, 1669, 1728, 2998, 3256
BstSFI CTRYAG 6 cut(s) 205, 1068, 2493, 2556, 2793, 3491
BstSLI GKGCMC 1 cut(s) 2473
BstUI CGCG 2 cut(s) 20, 62
BstV1I GCAGC 5 cut(s) 859, 1054, 2609, 2713, 3435
BstV2I GAAGAC 2 cut(s) 2506, 3153
BstX2I RGATCY 5 cut(s) 793, 1535, 2279, 2339, 2764
BstXI CCANNNNNNTGG 3 cut(s) 418, 1709, 1736
BstYI RGATCY 5 cut(s) 793, 1535, 2279, 2339, 2764
BsuRI GGCC 4 cut(s) 48, 303, 528, 2847
BtgI CCRYGG 1 cut(s) 3173
BtgZI GCGATG 2 cut(s) 560, 2385
BtrI CACGTC 2 cut(s) 1808, 3091
BtsI GCAGTG 2 cut(s) 3130, 3167
BtsIMutI CAGTG 7 cut(s) 1443, 1450, 2035, 2155, 3100, 3130, 3167
BtuMI TCGCGA 1 cut(s) 62
BveI ACCTGC 4 cut(s) 499, 973, 1958, 3473
Cac8I GCNNGC 5 cut(s) 1023, 1466, 1549, 1695, 2556
CfoI GCGC 4 cut(s) 1877, 2930, 3163, 3231
Cfr13I GGNCC 4 cut(s) 78, 301, 3389, 3408
CseI GACGC 3 cut(s) 9, 251, 1691
Csp6I GTAC 8 cut(s) 202, 555, 1947, 2310, 2354, 2523, 2640, 3296
CspCI CAANNNNNGTGG 2 cut(s) 3426, 3461
CviQI GTAC 8 cut(s) 202, 555, 1947, 2310, 2354, 2523, 2640, 3296
DdeI CTNAG 8 cut(s) 284, 1552, 1752, 2027, 2578, 2612, 2670, 3404
DinI GGCGCC 1 cut(s) 3230
DraI TTTAAA 4 cut(s) 15, 2443, 2506, 2802
Eam1104I CTCTTC 3 cut(s) 950, 1590, 1827
EarI CTCTTC 3 cut(s) 950, 1590, 1827
EciI GGCGGA 2 cut(s) 161, 3418
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 3 cut(s) 411, 1251, 2104
Eco24I GRGCYC 2 cut(s) 153, 599
Eco31I GGTCTC 1 cut(s) 62
Eco32I GATATC 1 cut(s) 66
Eco47I GGWCC 3 cut(s) 78, 3389, 3408
Eco47III AGCGCT 1 cut(s) 2929
Eco53kI GAGCTC 1 cut(s) 151
Eco57I CTGAAG 7 cut(s) 281, 1851, 1994, 2340, 2826, 2913, 3153
Eco88I CYCGRG 3 cut(s) 86, 532, 962
Eco91I GGTNACC 2 cut(s) 2694, 2993
EcoICRI GAGCTC 1 cut(s) 151
EcoNI CCTNNNNNAGG 1 cut(s) 1525
EcoO109I RGGNCCY 1 cut(s) 3408
EcoO65I GGTNACC 2 cut(s) 2694, 2993
EcoRII CCWGG 7 cut(s) 297, 514, 1520, 1669, 1728, 2998, 3256
EcoRV GATATC 1 cut(s) 66
EcoT14I CCWWGG 3 cut(s) 411, 1251, 2104
EcoT38I GRGCYC 2 cut(s) 153, 599
EgeI GGCGCC 1 cut(s) 3230
EheI GGCGCC 1 cut(s) 3230
ErhI CCWWGG 3 cut(s) 411, 1251, 2104
FaqI GGGAC 5 cut(s) 146, 302, 2825, 3231, 3375
FauI CCCGC 4 cut(s) 2340, 2987, 3402, 3441
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 2792
Fnu4HI GCNGC 6 cut(s) 873, 1068, 1389, 2598, 2727, 3424
FriOI GRGCYC 2 cut(s) 153, 599
Fsp4HI GCNGC 6 cut(s) 873, 1068, 1389, 2598, 2727, 3424
FspBI CTAG 5 cut(s) 378, 1161, 1944, 2834, 3075
FspI TGCGCA 1 cut(s) 1876
GlaI GCGC 4 cut(s) 1876, 2929, 3162, 3230
GluI GCNGC 6 cut(s) 873, 1068, 1389, 2598, 2727, 3424
GsaI CCCAGC 1 cut(s) 215
HaeII RGCGCY 2 cut(s) 2931, 3232
HaeIII GGCC 4 cut(s) 48, 303, 528, 2847
HapII CCGG 3 cut(s) 154, 199, 3283
HgaI GACGC 3 cut(s) 9, 251, 1691
HhaI GCGC 4 cut(s) 1877, 2930, 3163, 3231
Hin1I GRCGYC 2 cut(s) 243, 3229
Hin6I GCGC 4 cut(s) 1875, 2928, 3161, 3229
HinP1I GCGC 4 cut(s) 1875, 2928, 3161, 3229
HincII GTYRAC 2 cut(s) 404, 2651
HindII GTYRAC 2 cut(s) 404, 2651
HindIII AAGCTT 1 cut(s) 458
HpaII CCGG 3 cut(s) 154, 199, 3283
HphI GGTGA 9 cut(s) 511, 530, 997, 1685, 1892, 2231, 2658, 2688, 3197
Hpy99I CGWCG 4 cut(s) 25, 272, 329, 3040
HpyCH4IV ACGT 5 cut(s) 826, 1807, 2285, 3035, 3090
HpyF3I CTNAG 8 cut(s) 284, 1552, 1752, 2027, 2578, 2612, 2670, 3404
HpySE526I ACGT 5 cut(s) 826, 1807, 2285, 3035, 3090
Hsp92I GRCGYC 2 cut(s) 243, 3229
HspAI GCGC 4 cut(s) 1875, 2928, 3161, 3229
KasI GGCGCC 1 cut(s) 3228
Kpn2I TCCGGA 1 cut(s) 3282
Ksp22I TGATCA 1 cut(s) 118
LguI GCTCTTC 1 cut(s) 1590
LmnI GCTCC 4 cut(s) 148, 156, 2751, 3431
Lsp1109I GCAGC 5 cut(s) 859, 1054, 2609, 2713, 3435
LweI GCATC 8 cut(s) 1051, 1375, 1400, 1593, 2251, 3237, 3358, 3379
MaeI CTAG 5 cut(s) 378, 1161, 1944, 2834, 3075
MaeII ACGT 5 cut(s) 826, 1807, 2285, 3035, 3090
MfeI CAATTG 1 cut(s) 1847
MflI RGATCY 5 cut(s) 793, 1535, 2279, 2339, 2764
MhlI GDGCHC 4 cut(s) 153, 599, 1160, 2473
MluI ACGCGT 1 cut(s) 18
Mly113I GGCGCC 1 cut(s) 3229
MlyI GAGTC 4 cut(s) 78, 98, 967, 3213
MmeI TCCRAC 3 cut(s) 2166, 2590, 3246
MroI TCCGGA 1 cut(s) 3282
MroXI GAANNNNTTC 1 cut(s) 826
MslI CAYNNNNRTG 6 cut(s) 579, 1692, 2410, 3093, 3172, 3354
MspA1I CMGCKG 3 cut(s) 215, 819, 3387
MspI CCGG 3 cut(s) 154, 199, 3283
MspR9I CCNGG 8 cut(s) 199, 299, 516, 1522, 1671, 1730, 3000, 3258
MunI CAATTG 1 cut(s) 1847
Mva1269I GAATGC 2 cut(s) 423, 658
MvaI CCWGG 7 cut(s) 299, 516, 1522, 1671, 1730, 3000, 3258
MvnI CGCG 2 cut(s) 20, 62
NarI GGCGCC 1 cut(s) 3229
NciI CCSGG 1 cut(s) 199
NlaIV GGNNCC 4 cut(s) 2747, 3230, 3391, 3410
NmeAIII GCCGAG 2 cut(s) 271, 3008
NmuCI GTSAC 4 cut(s) 518, 2070, 2150, 2694
NruI TCGCGA 1 cut(s) 62
NsbI TGCGCA 1 cut(s) 1876
NspI RCATGY 5 cut(s) 337, 1468, 1517, 1697, 2026
OliI CACNNNNGTG 2 cut(s) 3093, 3172
PaeI GCATGC 2 cut(s) 1468, 1697
PaeR7I CTCGAG 3 cut(s) 86, 532, 962
PaqCI CACCTGC 1 cut(s) 973
PciI ACATGT 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 1590
PcsI WCGNNNNNNNCGW 2 cut(s) 2746, 3471
PctI GAATGC 2 cut(s) 423, 658
PdmI GAANNNNTTC 1 cut(s) 826
PfeI GAWTC 9 cut(s) 218, 767, 1417, 1733, 2711, 2782, 2933, 3003, 3301
PfoI TCCNGGA 1 cut(s) 1520
PkrI GCNGC 6 cut(s) 874, 1069, 1390, 2599, 2728, 3425
PleI GAGTC 4 cut(s) 78, 97, 966, 3213
PluTI GGCGCC 1 cut(s) 3232
PpsI GAGTC 4 cut(s) 78, 97, 966, 3213
Ppu21I YACGTR 1 cut(s) 2286
PpuMI RGGWCCY 1 cut(s) 3408
PscI ACATGT 1 cut(s) 333
PshAI GACNNNNGTC 1 cut(s) 88
PshBI ATTAAT 1 cut(s) 1062
PsiI TTATAA 1 cut(s) 2127
Psp124BI GAGCTC 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 3408
Psp6I CCWGG 7 cut(s) 297, 514, 1520, 1669, 1728, 2998, 3256
PspEI GGTNACC 2 cut(s) 2694, 2993
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 7 cut(s) 297, 514, 1520, 1669, 1728, 2998, 3256
PspN4I GGNNCC 4 cut(s) 2747, 3230, 3391, 3410
PspPI GGNCC 4 cut(s) 78, 301, 3389, 3408
PspPPI RGGWCCY 1 cut(s) 3408
PspXI VCTCGAGB 2 cut(s) 86, 532
PsrI GAACNNNNNNTAC 4 cut(s) 1732, 1764, 2346, 2378
PstI CTGCAG 2 cut(s) 1072, 2560
PsuI RGATCY 5 cut(s) 793, 1535, 2279, 2339, 2764
RruI TCGCGA 1 cut(s) 62
RsaI GTAC 8 cut(s) 203, 556, 1948, 2311, 2355, 2524, 2641, 3297
RsaNI GTAC 8 cut(s) 202, 555, 1947, 2310, 2354, 2523, 2640, 3296
RseI CAYNNNNRTG 6 cut(s) 579, 1692, 2410, 3093, 3172, 3354
SacI GAGCTC 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 1590
SatI GCNGC 6 cut(s) 873, 1068, 1389, 2598, 2727, 3424
Sau96I GGNCC 4 cut(s) 78, 301, 3389, 3408
SchI GAGTC 4 cut(s) 78, 98, 967, 3213
ScrFI CCNGG 8 cut(s) 199, 299, 516, 1522, 1671, 1730, 3000, 3258
SduI GDGCHC 4 cut(s) 153, 599, 1160, 2473
SfaNI GCATC 8 cut(s) 1051, 1375, 1400, 1593, 2251, 3237, 3358, 3379
SfcI CTRYAG 6 cut(s) 205, 1068, 2493, 2556, 2793, 3491
SfoI GGCGCC 1 cut(s) 3230
Sfr274I CTCGAG 3 cut(s) 86, 532, 962
SinI GGWCC 3 cut(s) 78, 3389, 3408
SlaI CTCGAG 3 cut(s) 86, 532, 962
SmiMI CAYNNNNRTG 6 cut(s) 579, 1692, 2410, 3093, 3172, 3354
SmlI CTYRAG 8 cut(s) 86, 532, 797, 900, 962, 1771, 2518, 2962
SmoI CTYRAG 8 cut(s) 86, 532, 797, 900, 962, 1771, 2518, 2962
SphI GCATGC 2 cut(s) 1468, 1697
SspDI GGCGCC 1 cut(s) 3228
SspI AATATT 2 cut(s) 1099, 2212
SspMI CTAG 5 cut(s) 378, 1161, 1944, 2834, 3075
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 8 cut(s) 197, 297, 514, 1520, 1669, 1728, 2998, 3256
StyI CCWWGG 3 cut(s) 411, 1251, 2104
TaiI ACGT 5 cut(s) 829, 1810, 2288, 3038, 3093
TatI WGTACW 2 cut(s) 2309, 3295
TauI GCSGC 1 cut(s) 1391
TfiI GAWTC 9 cut(s) 218, 767, 1417, 1733, 2711, 2782, 2933, 3003, 3301
TscAI CASTG 7 cut(s) 1443, 1450, 2035, 2155, 3100, 3130, 3174
TseFI GTSAC 4 cut(s) 518, 2070, 2150, 2694
TseI GCWGC 5 cut(s) 872, 1067, 2597, 2726, 3423
Tsp45I GTSAC 4 cut(s) 518, 2070, 2150, 2694
TspGWI ACGGA 5 cut(s) 313, 1388, 2738, 2929, 3463
TspRI CASTG 7 cut(s) 1443, 1450, 2035, 2155, 3100, 3130, 3174
VneI GTGCAC 1 cut(s) 2469
VpaK11BI GGWCC 3 cut(s) 78, 3389, 3408
VspI ATTAAT 1 cut(s) 1062
XagI CCTNNNNNAGG 1 cut(s) 1525
XceI RCATGY 5 cut(s) 337, 1468, 1517, 1697, 2026
XcmI CCANNNNNNNNNTGG 1 cut(s) 3171
XhoI CTCGAG 3 cut(s) 86, 532, 962
XmiI GTMKAC 1 cut(s) 2792
XmnI GAANNNNTTC 1 cut(s) 826
XspI CTAG 5 cut(s) 378, 1161, 1944, 2834, 3075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.