Rh6DG332900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016760
Physical Location & Seq
Forward (+)
53791583 .. 53795729
4147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG332900.1

Sequence Viewer

Length: 2862 bp
ATGGACTCGATTTTAAACTCATCCTTGCAAGAAATCTGCTCGGAAGGTCCAAACGGTCTCCCTCTTCAAACCCTCTCCTCCAGGCTCAACCTCTCTCCGCCACTCCAACAACCCCTATGGGCGGCGCTACTCTCCGTCCCCGCCCTCAAATTCCACACCCAAACCCAAAACGCCACCGTTTCGCACCTCCCCACAGACCCTTCAATCCAGTCGTTCCGCGACGCCGAGAAGCTCAACCTGAAGCTCGTCGCCGACCAGCCTCTCCGCAACAACTTCCTCGGCCTCTACGACGTCCAGTCCGCATCCGACACCATGTGCGAGTACCAACGCAAAACCCTAGAGCGCGTGGCCGCGGCTGGGTCAAAGGGGATCACGCAGAGTCAACTATCCAAGGAGCTTGGGATTAGAGGTGAGAATTTCTTTTACAAATTGAGGAGGCTCGAATGTCGAGGCTTGATTGTGAAGCAACCATTGAATGATGATCATGATGATGATGCCAATGTGAGTGGGAGTACTAATTTAGTGTACTTAAATCGTTATGCAAAGCATCTAGGTCTAGGGTCTGAACAAAAAGCTGAGGCCACTAAATCTAAGGAGGAAGAGAATATGACTAAGAAGAGTGGTAATTTGATAGCGGAACTTCCGATTGAGCATCAAATTTACAAGCTAGTTAATGATGCGGGGTCAAGGGGGGTCATGGTGTTTAAGGTTGCAGAGAGGCTGGGAATTGATAGGAAAGAGAATAGGAAGAGGATTGAAAGTTTGTGCTCCAAGTTTGGGATGATAATGGATAAAGAGAAACACGGAAAGACGCAAGTGTATCGAGTTTGGGCACCTGGGAAACGTAATGCTGAATCAGCTAATGCAATTCTGAATGAGTCTGAAAATGTTACTCAAAGTAAATTTAATGAAGATGTTCTTCGTGGGTCCGTTCAGAAAGGTGATACAGCAATAAATGATGTCTCATCAGGAATGCCACCTGCTGCGGAATGTGCTAGGCCCAGAAGGGGAAAAAGTGTACTTGAACCATCACAGGATAAAGGGGATGAACGTCAATCTCTGGGAATTAGAAGCCGTTTGCAGAAAAGAAAGAGATCTTCTGAAGCAGCTTCTGTAAAGTTTATAGAAGTTGATGGCGTAACTGAGCACCGACCTGGACCAAATGAAGATGGTGAGGCGAGGTGTGCTTTTTCAAAGTCAAAATCAACATGTCAAAGAAGGTTTTTATGGACAGAGGAGGCTGATAGGCAATTAGTCATCCAATATGTAAGACGGTGTGCAACTCTTGGGGCAAAATATCATCACCAGATAAATTGGGCTTCACTTCCGGACCTTCCAGCGCCTCCAAGTACCTGCAAAAGAAGAATGTCCTATCTGAAGCTCAGTAATGGAAAATTTAGAAAATCTTTAATGACACTGTGTAACATGCTCAGTAAACGGTATGCAAAGCTCCTGCAGAAAACCCAGAACAGGTCATTTTACCAAGATGATTGCGGATCACTTTTGCAAGGTTCAACAGAGGAGGGTTGTAACCAGAATTATCCTCTCAGTGACCGTGGGACAGGTCTTCAGGAAGAACAATGGGATGACTTTAATGACAAAAATATTAAAGAAGTGCTTGATCAGATACTTCACTATGTGAGGTTGACTAAATCTGTTGCCTCCAAAAGATGTGGAGCTTCCTGCGAGGAGGGTTCAGATCTTAATGCTAATGCTGAAGATGTTGGAGGCAGTGATTGTGAACCTTTGTCTCTCTCACCTCAGGTCTTCCCCAATATTTGCAAGTCACCATTTCCAGCTGATTCTGGCAAGAGAGCTGCCAAATTTGCTCATTGGCTCCACAAAAGAGATAGAGATCTTATGGGAAGGGGGCTTGATCTTCCTACAGCTATACAGTGTGGTGAACTTTTTCATTTGTTTGCTCTAGTTTTTTCAGGTGAATTGTCAATCTCCCCATGCTTGCCAGATGGAGTGGGAGAAGCAGAAGATTTGAGAAGCTCAAAACATAAAATTGGTAGGAATAAACATTTGGATGCTAAAAGACTAAAATCCTTGGTTGCAGAAAGTGAGATTATTTCCCGTCGAGAAAAAGGTTTCCCGGGTCTCATGGTATCTGTATATCGTACAGCATCTTCTAAAGCCTATTGTGAAAATTTGTTCAAAGATGATGATACTCTTACCGGTGACAAGCATTGTGGTGAAAATTATCAGCAAGACAGTACTTCAGGTATGAATCAGATTAGAGGAAACCTCAATTCAGGCATTGTTATCCCTGTGGCAGAGACCTGTAGTGAATCAGCTGGCGAAGGTATCCTCCATCCTGGAAATTGTGATATTGGTGGAACTCTTTTGATAGGGAATGCAAATATGAATGTTAATGATGCACATAATGTGACTATTCTTAATTCTCCTAAAGAGGTTGCTGAGCGTCCATCTGAAAAACTAACCAGCGGTGTGGAAGGCAGAGTTATCTCACCTAGAGGAGATTTTGATGAATGTTCTAAACTCTCTTCTGATGAATTATGTGTGCCACTTCTTCCTTGGATAAATGGGGACGAGACCATCAACAAGACTATCTACGAGGGGTTCATGCAACGTGTTTTTGGGATTGTGATGCAAAACCCCGGGATATTAGAGGGTGAAATTATCCGTAAGATGGATGTACTGAATCCGCAGAGTTGTGGAAAATTGTTGGCGGAGATGATTGAAGCAAAACATCTTCATGTCAGGACAATGCGCCAAGCCACATCCAATGGCCCCCCAGCATTTCTAGGGAACCTATTAGGAAGCAGCCTCACCCAATCAAAGGTGGTTTTCCGTAGGCATCTCTTTGCAAATTCCATGAGCGCGTTCTTGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

953

Amino Acids

105.63

Weight (kDa)

8.4

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 96 5.1e-25 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 108 - 182 1.1e-13 B-block binding subunit of TFIIIC
DUF7646 PF24657 209 - 285 6.1e-28 Domain of unknown function (DUF7646)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23920 FvH4_2g23920
malus_domestica MD11G1308600.v1.1
prunus_persica Prupe.8G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291551
rosa_laevigata RLG00000012108
rosa_multiflora Rmu_sc0002226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175980
rosa_rugosa Rorug06G0220200
rosa_samantha Rh6AG332400 Rh6BG339300 Rh6CG345600 Rh6DG332900
rosa_wichuraiana Rw6G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 988
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 2 cut(s) 988, 1361
AccB1I GGYRCC 1 cut(s) 832
AccII CGCG 4 cut(s) 219, 345, 353, 2849
AccIII TCCGGA 1 cut(s) 1327
AclWI GGATC 2 cut(s) 377, 1504
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 8 cut(s) 149, 415, 657, 902, 1394, 1823, 2152, 2836
AcuI CTGAAG 6 cut(s) 260, 1122, 1397, 1553, 1737, 2208
AcyI GRCGYC 2 cut(s) 222, 291
AdeI CACNNNGTG 2 cut(s) 315, 1639
AfaI GTAC 8 cut(s) 323, 514, 527, 1020, 1351, 2125, 2221, 2664
AfiI CCNNNNNNNGG 7 cut(s) 121, 357, 777, 1007, 1470, 2656, 2806
AflIII ACRYGT 2 cut(s) 1208, 2596
AgeI ACCGGT 1 cut(s) 2180
AgsI TTSAA 9 cut(s) 68, 204, 475, 758, 1025, 1194, 1515, 2161, 2708
AhdI GACNNNNNGTC 1 cut(s) 295
AjnI CCWGG 4 cut(s) 80, 835, 1153, 2320
AjuI GAANNNNNNNTTGG 2 cut(s) 2012, 2044
Alw21I GWGCWC 2 cut(s) 770, 1149
Alw26I GTCTC 6 cut(s) 62, 967, 1755, 2108, 2276, 2552
AlwI GGATC 2 cut(s) 377, 1504
AlwNI CAGNNNCTG 1 cut(s) 1112
Ama87I CYCGRG 2 cut(s) 2098, 2624
Aor13HI TCCGGA 1 cut(s) 1327
AoxI GGCC 5 cut(s) 280, 348, 579, 998, 2755
ApeKI GCWGC 4 cut(s) 983, 1106, 1817, 2790
ApoI RAATTY 8 cut(s) 149, 415, 657, 902, 1394, 1823, 2152, 2836
AsiGI ACCGGT 1 cut(s) 2180
Asp700I GAANNNNTTC 2 cut(s) 915, 1908
AspLEI GCGC 5 cut(s) 127, 345, 1342, 2739, 2849
AspS9I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2756
AsuC2I CCSGG 4 cut(s) 2099, 2100, 2625, 2626
AvaI CYCGRG 2 cut(s) 2098, 2624
AvaII GGWCC 4 cut(s) 47, 927, 1157, 1330
AxyI CCTNAGG 1 cut(s) 1761
BaeGI GKGCMC 1 cut(s) 835
BanI GGYRCC 1 cut(s) 832
BarI GAAGNNNNNNTAC 2 cut(s) 2116, 2148
BbsI GAAGAC 2 cut(s) 1559, 1759
Bbv12I GWGCWC 2 cut(s) 770, 1149
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 4 cut(s) 970, 1118, 1804, 2802
BccI CCATC 8 cut(s) 1036, 1127, 1163, 1961, 2325, 2440, 2570, 2650
BceAI ACGGC 1 cut(s) 1059
BcgI CGANNNNNNTGC 2 cut(s) 803, 837
BciT130I CCWGG 4 cut(s) 82, 837, 1155, 2322
BciVI GTATCC 1 cut(s) 2321
BclI TGATCA 2 cut(s) 481, 1621
BcnI CCSGG 4 cut(s) 2099, 2100, 2625, 2626
BcoDI GTCTC 6 cut(s) 62, 967, 1755, 2108, 2276, 2552
BfaI CTAG 8 cut(s) 338, 551, 557, 668, 996, 1925, 2478, 2771
BfmI CTRYAG 4 cut(s) 1454, 1884, 2287, 2858
BfoI RGCGCY 2 cut(s) 128, 1343
BfuAI ACCTGC 2 cut(s) 988, 1361
BfuI GTATCC 1 cut(s) 2321
BglII AGATCT 3 cut(s) 1094, 1699, 1855
BisI GCNGC 7 cut(s) 123, 351, 354, 984, 1107, 1818, 2791
BlpI GCTNAGC 1 cut(s) 2424
BlsI GCNGC 7 cut(s) 124, 352, 355, 985, 1108, 1819, 2792
BmcAI AGTACT 2 cut(s) 514, 2221
Bme1390I CCNGG 8 cut(s) 82, 837, 1155, 2099, 2100, 2322, 2625, 2626
Bme18I GGWCC 4 cut(s) 47, 927, 1157, 1330
BmeRI GACNNNNNGTC 1 cut(s) 295
BmeT110I CYCGRG 2 cut(s) 2098, 2624
BmgT120I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2756
BmiI GGNNCC 5 cut(s) 834, 928, 1838, 2758, 2777
BmrFI CCNGG 8 cut(s) 82, 837, 1155, 2099, 2100, 2322, 2625, 2626
BpiI GAAGAC 2 cut(s) 1559, 1759
BplI GAGNNNNNCTC 2 cut(s) 2235, 2267
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 2424
BpuMI CCSGG 4 cut(s) 2099, 2100, 2625, 2626
BsaBI GATNNNNATC 1 cut(s) 1854
BsaHI GRCGYC 2 cut(s) 222, 291
BsaI GGTCTC 4 cut(s) 62, 2108, 2276, 2552
BsaWI WCCGGW 2 cut(s) 1327, 2180
Bsc4I CCNNNNNNNGG 7 cut(s) 121, 357, 777, 1007, 1470, 2656, 2806
Bse118I RCCGGY 1 cut(s) 2180
Bse1I ACTGG 2 cut(s) 208, 295
Bse21I CCTNAGG 1 cut(s) 1761
Bse8I GATNNNNATC 1 cut(s) 1854
BseAI TCCGGA 1 cut(s) 1327
BseBI CCWGG 4 cut(s) 82, 837, 1155, 2322
BseJI GATNNNNATC 1 cut(s) 1854
BseLI CCNNNNNNNGG 7 cut(s) 121, 357, 777, 1007, 1470, 2656, 2806
BseMII CTCAG 7 cut(s) 567, 1134, 1396, 1444, 1561, 1775, 2415
BseNI ACTGG 2 cut(s) 208, 295
BseRI GAGGAG 6 cut(s) 67, 448, 1250, 1535, 1703, 2496
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 4 cut(s) 970, 1118, 1804, 2802
BseYI CCCAGC 3 cut(s) 356, 721, 2761
Bsh1236I CGCG 4 cut(s) 219, 345, 353, 2849
BshFI GGCC 5 cut(s) 282, 350, 581, 1000, 2757
BshNI GGYRCC 1 cut(s) 832
BshTI ACCGGT 1 cut(s) 2180
BsiHKAI GWGCWC 2 cut(s) 770, 1149
BsiHKCI CYCGRG 2 cut(s) 2098, 2624
BsiSI CCGG 4 cut(s) 1328, 2099, 2181, 2625
BslFI GGGAC 3 cut(s) 122, 1573, 2567
BslI CCNNNNNNNGG 7 cut(s) 121, 357, 777, 1007, 1470, 2656, 2806
BsmAI GTCTC 6 cut(s) 62, 967, 1755, 2108, 2276, 2552
BsmFI GGGAC 3 cut(s) 122, 1573, 2567
BsmI GAATGC 2 cut(s) 978, 2365
BsnI GGCC 5 cut(s) 282, 350, 581, 1000, 2757
Bso31I GGTCTC 4 cut(s) 62, 2108, 2276, 2552
BsoBI CYCGRG 2 cut(s) 2098, 2624
Bsp1286I GDGCHC 3 cut(s) 770, 835, 1149
Bsp13I TCCGGA 1 cut(s) 1327
Bsp143I GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
Bsp1720I GCTNAGC 1 cut(s) 2424
BspANI GGCC 5 cut(s) 282, 350, 581, 1000, 2757
BspCNI CTCAG 7 cut(s) 568, 1135, 1395, 1443, 1560, 1774, 2416
BspEI TCCGGA 1 cut(s) 1327
BspFNI CGCG 4 cut(s) 219, 345, 353, 2849
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 5 cut(s) 834, 928, 1838, 2758, 2777
BspMAI CTGCAG 1 cut(s) 1458
BspMI ACCTGC 2 cut(s) 988, 1361
BspPI GGATC 2 cut(s) 377, 1504
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 4 cut(s) 62, 2108, 2276, 2552
BsrFI RCCGGY 1 cut(s) 2180
BsrI ACTGG 2 cut(s) 208, 295
BssAI RCCGGY 1 cut(s) 2180
BssMI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
BssNI GRCGYC 2 cut(s) 222, 291
BssT1I CCWWGG 3 cut(s) 390, 2052, 2540
Bst2UI CCWGG 4 cut(s) 82, 837, 1155, 2322
Bst4CI ACNGT 8 cut(s) 56, 178, 1275, 1419, 1440, 1556, 1896, 2219
Bst6I CTCTTC 5 cut(s) 69, 594, 611, 743, 2515
BstACI GRCGYC 2 cut(s) 222, 291
BstC8I GCNNGC 2 cut(s) 1961, 2302
BstDEI CTNAG 9 cut(s) 576, 591, 612, 1143, 1382, 1430, 1547, 1761, 2424
BstDSI CCRYGG 2 cut(s) 351, 1555
BstFNI CGCG 4 cut(s) 219, 345, 353, 2849
BstH2I RGCGCY 2 cut(s) 128, 1343
BstHHI GCGC 5 cut(s) 127, 345, 1342, 2739, 2849
BstKTI GATC 8 cut(s) 372, 484, 1097, 1499, 1624, 1702, 1858, 1879
BstMAI GTCTC 6 cut(s) 62, 967, 1755, 2108, 2276, 2552
BstMBI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
BstMWI GCNNNNNNNGC 4 cut(s) 857, 992, 1184, 1826
BstNI CCWGG 4 cut(s) 82, 837, 1155, 2322
BstNSI RCATGY 2 cut(s) 1212, 1429
BstSCI CCNGG 8 cut(s) 80, 835, 1153, 2097, 2098, 2320, 2623, 2624
BstSFI CTRYAG 4 cut(s) 1454, 1884, 2287, 2858
BstSLI GKGCMC 1 cut(s) 835
BstUI CGCG 4 cut(s) 219, 345, 353, 2849
BstV1I GCAGC 4 cut(s) 970, 1118, 1804, 2802
BstV2I GAAGAC 2 cut(s) 1559, 1759
BstX2I RGATCY 3 cut(s) 1094, 1699, 1855
BstXI CCANNNNNNTGG 1 cut(s) 2455
BstYI RGATCY 3 cut(s) 1094, 1699, 1855
Bsu36I CCTNAGG 1 cut(s) 1761
BsuI GTATCC 1 cut(s) 2321
BsuRI GGCC 5 cut(s) 282, 350, 581, 1000, 2757
BtgI CCRYGG 2 cut(s) 351, 1555
BtsI GCAGTG 1 cut(s) 1738
BtsIMutI CAGTG 4 cut(s) 1415, 1555, 1738, 1901
BveI ACCTGC 2 cut(s) 988, 1361
Cac8I GCNNGC 2 cut(s) 1961, 2302
CaiI CAGNNNCTG 1 cut(s) 1112
CciI TCATGA 1 cut(s) 484
CfoI GCGC 5 cut(s) 127, 345, 1342, 2739, 2849
Cfr10I RCCGGY 1 cut(s) 2180
Cfr13I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2756
Cfr42I CCGCGG 1 cut(s) 354
Cfr9I CCCGGG 2 cut(s) 2098, 2624
CseI GACGC 3 cut(s) 230, 820, 2417
Csp6I GTAC 8 cut(s) 322, 513, 526, 1019, 1350, 2124, 2220, 2663
CspAI ACCGGT 1 cut(s) 2180
CspCI CAANNNNNGTGG 6 cut(s) 487, 522, 1654, 1689, 2176, 2211
CviQI GTAC 8 cut(s) 322, 513, 526, 1019, 1350, 2124, 2220, 2663
DdeI CTNAG 9 cut(s) 576, 591, 612, 1143, 1382, 1430, 1547, 1761, 2424
DpnI GATC 8 cut(s) 371, 483, 1096, 1498, 1623, 1701, 1857, 1878
DpnII GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 2 cut(s) 315, 1639
DriI GACNNNNNGTC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 5 cut(s) 69, 594, 611, 743, 2515
Eam1105I GACNNNNNGTC 1 cut(s) 295
EarI CTCTTC 5 cut(s) 69, 594, 611, 743, 2515
EciI GGCGGA 2 cut(s) 87, 2711
Eco130I CCWWGG 3 cut(s) 390, 2052, 2540
Eco31I GGTCTC 4 cut(s) 62, 2108, 2276, 2552
Eco47I GGWCC 4 cut(s) 47, 927, 1157, 1330
Eco57I CTGAAG 6 cut(s) 260, 1122, 1397, 1553, 1737, 2208
Eco81I CCTNAGG 1 cut(s) 1761
Eco88I CYCGRG 2 cut(s) 2098, 2624
EcoRII CCWGG 4 cut(s) 80, 835, 1153, 2320
EcoT14I CCWWGG 3 cut(s) 390, 2052, 2540
ErhI CCWWGG 3 cut(s) 390, 2052, 2540
FalI AAGNNNNNCTT 4 cut(s) 903, 935, 1602, 1634
FaqI GGGAC 3 cut(s) 122, 1573, 2567
FauI CCCGC 2 cut(s) 148, 673
FbaI TGATCA 2 cut(s) 481, 1621
Fnu4HI GCNGC 7 cut(s) 123, 351, 354, 984, 1107, 1818, 2791
Fsp4HI GCNGC 7 cut(s) 123, 351, 354, 984, 1107, 1818, 2791
FspBI CTAG 8 cut(s) 338, 551, 557, 668, 996, 1925, 2478, 2771
GlaI GCGC 5 cut(s) 126, 344, 1341, 2738, 2848
GluI GCNGC 7 cut(s) 123, 351, 354, 984, 1107, 1818, 2791
GsaI CCCAGC 3 cut(s) 360, 725, 2765
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 2 cut(s) 128, 1343
HaeIII GGCC 5 cut(s) 282, 350, 581, 1000, 2757
HapII CCGG 4 cut(s) 1328, 2099, 2181, 2625
HgaI GACGC 3 cut(s) 230, 820, 2417
HhaI GCGC 5 cut(s) 127, 345, 1342, 2739, 2849
Hin1I GRCGYC 2 cut(s) 222, 291
Hin6I GCGC 5 cut(s) 125, 343, 1340, 2737, 2847
HinP1I GCGC 5 cut(s) 125, 343, 1340, 2737, 2847
HincII GTYRAC 2 cut(s) 383, 1647
HindII GTYRAC 2 cut(s) 383, 1647
HinfI GANTC 8 cut(s) 5, 379, 854, 878, 1802, 2233, 2294, 2668
HpaII CCGG 4 cut(s) 1328, 2099, 2181, 2625
Hpy166II GTNNAC 7 cut(s) 383, 526, 1019, 1436, 1647, 1742, 1904
Hpy188III TCNNGA 6 cut(s) 485, 969, 1328, 1571, 2084, 2728
Hpy8I GTNNAC 7 cut(s) 383, 526, 1019, 1436, 1647, 1742, 1904
Hpy99I CGWCG 4 cut(s) 224, 251, 293, 2085
HpyAV CCTTC 8 cut(s) 38, 210, 999, 1212, 1343, 1860, 2300, 2453
HpyCH4III ACNGT 8 cut(s) 56, 178, 1275, 1419, 1440, 1556, 1896, 2219
HpyCH4IV ACGT 4 cut(s) 291, 844, 1051, 2596
HpyF10VI GCNNNNNNNGC 4 cut(s) 857, 992, 1184, 1826
HpyF3I CTNAG 9 cut(s) 576, 591, 612, 1143, 1382, 1430, 1547, 1761, 2424
HpySE526I ACGT 4 cut(s) 291, 844, 1051, 2596
Hsp92I GRCGYC 2 cut(s) 222, 291
HspAI GCGC 5 cut(s) 125, 343, 1340, 2737, 2847
Kpn2I TCCGGA 1 cut(s) 1327
Ksp22I TGATCA 2 cut(s) 481, 1621
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
LmnI GCTCC 5 cut(s) 394, 773, 1455, 1676, 1842
Lsp1109I GCAGC 4 cut(s) 970, 1118, 1804, 2802
MaeI CTAG 8 cut(s) 338, 551, 557, 668, 996, 1925, 2478, 2771
MaeII ACGT 4 cut(s) 291, 844, 1051, 2596
MaeIII GTNAC 8 cut(s) 889, 1138, 1421, 1529, 1550, 1785, 2183, 2392
MalI GATC 8 cut(s) 371, 483, 1096, 1498, 1623, 1701, 1857, 1878
MboI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
MflI RGATCY 3 cut(s) 1094, 1699, 1855
MhlI GDGCHC 3 cut(s) 770, 835, 1149
MlyI GAGTC 2 cut(s) 388, 887
MmeI TCCRAC 3 cut(s) 130, 330, 1705
MroI TCCGGA 1 cut(s) 1327
MroXI GAANNNNTTC 2 cut(s) 915, 1908
MslI CAYNNNNRTG 4 cut(s) 489, 2031, 2196, 2721
MspA1I CMGCKG 4 cut(s) 353, 1799, 2300, 2451
MspI CCGG 4 cut(s) 1328, 2099, 2181, 2625
MspR9I CCNGG 8 cut(s) 82, 837, 1155, 2099, 2100, 2322, 2625, 2626
Mva1269I GAATGC 2 cut(s) 978, 2365
MvaI CCWGG 4 cut(s) 82, 837, 1155, 2322
MvnI CGCG 4 cut(s) 219, 345, 353, 2849
MwoI GCNNNNNNNGC 4 cut(s) 857, 992, 1184, 1826
NciI CCSGG 4 cut(s) 2099, 2100, 2625, 2626
NdeII GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
NlaIV GGNNCC 5 cut(s) 834, 928, 1838, 2758, 2777
NmeAIII GCCGAG 2 cut(s) 250, 258
NmuCI GTSAC 4 cut(s) 1550, 1785, 2183, 2392
NspI RCATGY 2 cut(s) 1212, 1429
PagI TCATGA 1 cut(s) 484
PaqCI CACCTGC 1 cut(s) 988
PciI ACATGT 1 cut(s) 1208
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 2 cut(s) 978, 2365
PdmI GAANNNNTTC 2 cut(s) 915, 1908
PfeI GAWTC 5 cut(s) 854, 1802, 2233, 2294, 2668
PfoI TCCNGGA 1 cut(s) 2320
PinAI ACCGGT 1 cut(s) 2180
PkrI GCNGC 7 cut(s) 124, 352, 355, 985, 1108, 1819, 2792
PleI GAGTC 2 cut(s) 387, 886
PpsI GAGTC 2 cut(s) 387, 886
PscI ACATGT 1 cut(s) 1208
Psp6I CCWGG 4 cut(s) 80, 835, 1153, 2320
PspFI CCCAGC 3 cut(s) 356, 721, 2761
PspGI CCWGG 4 cut(s) 80, 835, 1153, 2320
PspN4I GGNNCC 5 cut(s) 834, 928, 1838, 2758, 2777
PspPI GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2756
PstI CTGCAG 1 cut(s) 1458
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 3 cut(s) 1094, 1699, 1855
PvuII CAGCTG 2 cut(s) 1799, 2300
RsaI GTAC 8 cut(s) 323, 514, 527, 1020, 1351, 2125, 2221, 2664
RsaNI GTAC 8 cut(s) 322, 513, 526, 1019, 1350, 2124, 2220, 2663
RseI CAYNNNNRTG 4 cut(s) 489, 2031, 2196, 2721
SacII CCGCGG 1 cut(s) 354
SatI GCNGC 7 cut(s) 123, 351, 354, 984, 1107, 1818, 2791
Sau3AI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
Sau96I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2756
ScaI AGTACT 2 cut(s) 514, 2221
SchI GAGTC 2 cut(s) 388, 887
ScrFI CCNGG 8 cut(s) 82, 837, 1155, 2099, 2100, 2322, 2625, 2626
SduI GDGCHC 3 cut(s) 770, 835, 1149
SfcI CTRYAG 4 cut(s) 1454, 1884, 2287, 2858
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
SinI GGWCC 4 cut(s) 47, 927, 1157, 1330
SmaI CCCGGG 2 cut(s) 2100, 2626
SmiMI CAYNNNNRTG 4 cut(s) 489, 2031, 2196, 2721
SspI AATATT 2 cut(s) 1606, 1777
SspMI CTAG 8 cut(s) 338, 551, 557, 668, 996, 1925, 2478, 2771
StyD4I CCNGG 8 cut(s) 80, 835, 1153, 2097, 2098, 2320, 2623, 2624
StyI CCWWGG 3 cut(s) 390, 2052, 2540
TaaI ACNGT 8 cut(s) 56, 178, 1275, 1419, 1440, 1556, 1896, 2219
TaiI ACGT 4 cut(s) 294, 847, 1054, 2599
TaqI TCGA 5 cut(s) 8, 441, 448, 823, 2083
TatI WGTACW 5 cut(s) 512, 525, 1018, 2219, 2662
TauI GCSGC 3 cut(s) 125, 353, 356
TfiI GAWTC 5 cut(s) 854, 1802, 2233, 2294, 2668
TscAI CASTG 4 cut(s) 1422, 1555, 1738, 1901
TseFI GTSAC 4 cut(s) 1550, 1785, 2183, 2392
TseI GCWGC 4 cut(s) 983, 1106, 1817, 2790
Tsp45I GTSAC 4 cut(s) 1550, 1785, 2183, 2392
TspGWI ACGGA 5 cut(s) 124, 819, 919, 2639, 2807
TspMI CCCGGG 2 cut(s) 2098, 2624
TspRI CASTG 4 cut(s) 1422, 1555, 1738, 1901
VpaK11BI GGWCC 4 cut(s) 47, 927, 1157, 1330
XapI RAATTY 8 cut(s) 149, 415, 657, 902, 1394, 1823, 2152, 2836
XceI RCATGY 2 cut(s) 1212, 1429
XcmI CCANNNNNNNNNTGG 1 cut(s) 2538
XmaI CCCGGG 2 cut(s) 2098, 2624
XmnI GAANNNNTTC 2 cut(s) 915, 1908
XspI CTAG 8 cut(s) 338, 551, 557, 668, 996, 1925, 2478, 2771
ZraI GACGTC 1 cut(s) 292
ZrmI AGTACT 2 cut(s) 514, 2221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.