Rroxscaffold_7G00175980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016760
Physical Location & Seq
Reverse (-)
15458759 .. 15463214
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175980.1

Sequence Viewer

Length: 2961 bp
ATGGACTCGATTTTAAACTCATCCTTGCAAGAAATCTGCTCGGAAGGTCCAAACGGTCTCCCTCTTCAAACCCTCTCCTCCAGGCTCAACCTCTCTCCGCCACTCCAACAACACCTATGGGCGGCGCTACTCTCCGTCCCCGCCCTCAAATTCCACACCCAAACCCAAAACGCCACCGTTTCGCACCTCCCCACAGACCCTTCAATCCAGTCGTTCCGCGACGCCGAGAAGCTCAACCTGAAGCTCGTCGCCGACCAGCCTCTCCGCAACAACTTCCTCGGCCTCTACGACGTCCAGTCCGCATCCGACACCATGTGCGAGTACCAACGCAAAACCCTAGAGCGCGTGGCCGCGGCTGGGTCAAAGGGGATCACGCAGAGTCAACTCTCCAAGGAGCTTGGGATTAGAGGTGAGAATTTCTTTTACAAATTGAGGAGGCTTGAATGTCAGGGCTTGATTGTGAAGCAACCATTGAATGATGATCATGATGATGATGCCAATGTGAGTGGGAGTACTAATTTAGTGTACTTAAATCGTTATGCAAAGCATTTAGGTCTAGGGTCTGAACAAAAAGCTGAGGCCACTAAATCTAAGGAGGAAGAGAATATGACTAAGAAGAGTGGTAATTTGATAGCGGAACTTCCGATTGAGCATCAAATTTACGAGCTAGTTAATGATGCGGGGTCAAGGGGGGTCATGGTGTTTAAGGTTGCAGAGAGGCTGGGAATTGATAGGAAAGAGAATAGGAAGAGGATTGAAGGTTTGTGCTCCAAGTTTGGGATGATAATGGATAAAGAGAAATGCGGAAAGACCAAAGTGTATCGAGTTTGGGCACCTGGGAAACGTAATGCTGAATCAGCTAATGCAATTCTGAATGAGTCTGGAAATGTTACTCAAAGTAAATTTAATGAAGATGTACTTCGTGGGTCCGTTCAGAAAGGTGATACAGCAATGGCAACAGCAATAAATGATGTCTCATCAGGAATGCCACCTGCTGTGGAATGTGCTAGGCCCAGAAGGGGAAAAAGTGTACTTGAACCATCACAGCATATGTCGGATTACACTTTAACTTCATTCATTAGAGGTAGTCATTCACAAAATATACAGACTTCTGTTTCATGCATGTATGCTCCTGATAAAGGGGATGAACGTCAATCTCTGGGAATTAGAAGCCCTTTGCAGAAAAGAAAGAGATCTTCTGAAGCAGCTTCTGTAAAGTTTATAGAAGTTGATGGCGTGACTGAGCACCAACCTGGACCAAATGAAGGTGGTGAGGCGAGGTGTGCTTTTTCAAAGTCAAAATCAACATGTCAAAGAAGGTTTTTATGGACAGAGGAGGCTGATAGGCACTTAGTTATCCAATATGTAAGACGGTGTGCAACTCTTGGGGCAAAATATCATCACCAGATAAATTGGGCTTTAATTCCGGACCTTCCAGCGCCTCCAAGTACCTGCAAAAGAAGAATGTCATATCTGAAACTCAGTAATGGAAAATTTAGAAAATCTTTAATGACACTGTGTAACATGCTCAGTAAACGGTATGCAAAGCTCCTGCAGAAAACCCAGAACAGGTCATTTTACCAAGATGATTGCGGATCACTTTTGCAAGGTTCAACAGAGGAAGGTTGTAACCAGAATTATCCTCTCAGTGACCATGGGACAGGTCTTCAGGAAGAACAATGGGATGACTTTAATGACAAAAATATTAAAGAAGTGCTTGATCAGATACTTCACTATGTGAGGTTGACTAAATCTGTTGCCTCCAAAAGATGTGGAGCTTCCTGCGAGGAGGGTTCAGATCTTAATGCTAATGCTGAAGATGTTGAAGGCAGTGATTGTGAACCTTTGTCTCTCTCACCTCAGGTCTTCCCCAATATTTGCAAGTCACCATTTCCAACTGATTCTGGCAAGAGAGCTGCCAAATTTGCTCATTGGCTGCACAAAAGAGATAGAGATATTATGGGAAGGGGGCTTGATCTTCCTACAGATATACAGTGTGGTGAACTTTTTCAATTGTTTGCTCTAGTTTTTTCAGGTGAATTGTCAATCTCCCCATGCGTGCCAGATGGAGTGGGAGAAGCAGAAGATTTGAGAAGCTCAAAACATAAAATTGGTAGGAATAAACATTTGGATGCTAAAAGACTAAAATCCTTGGTTGCAGAAAGTGAGATTATTTCCCGTCGAGAAAAAGGTTTCCCGGGTCTCATGGTATCTGTATGTCGTACAGCATCTTCTAAAGCCTATTGTGAAAATTTGTTCAAAGATGATGATACTCTTACCGGTGACAAACATTGTGGTGAAAATTATCAGCAAGACAATACTTCAGGTATGAATCAGATTAGAGGAAACCTCAATTCAGGCATTGTTATCACTGTGGCAGAGACCTGTAGTGAATCAGCTGGCGAAGGTATCCTCCATCCTGGAAATTGTGATATTGGTGGAACTCTTTTGATAGGGAAAACAAATATGAATGTTAATGATGCACATAATGTGACTATTCTTAATTCTCCTAAAGAGGTTGCTGAGCGTCCGTCTGAAAAACTAACCAGCGGTGTGGAAGGCAAAGTTATCTCACCTAGAGGAGATTTTGACGAATGTTCTAAACTCTCTTCTGATGAATTATGTGTGCCACTTCTTCCTTGGATAAATGGGGACGAGACCATCAACAGGACTATCTATGAGGGGTTCATGCAATGTGTTTTTGGGATTGTGATGCAAAACCCTGGGATATTAGAGGGTGAAATTATCCGCAAGATGGATGTACTGAATCCGCAGAGTTGTAGAAAATTGTTGGTGGAGATGATTGAAGCAAAACATCTTCATGTCAGGACAATGCGCCAAGCCACATCGAATGGCCCCCCAGCATTTCTAGGGAACCTATTAGGAAGCAGCCTCACCCAATCGAAGGTGGTTTTCCGAAGGCATCTCTTTGCAAATTCCAGGAGCGCGTTCTTGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

986

Amino Acids

109.26

Weight (kDa)

8.1

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 96 1.7e-25 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 108 - 182 4.7e-14 B-block binding subunit of TFIIIC
DUF7646 PF24657 209 - 285 2.6e-27 Domain of unknown function (DUF7646)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23920 FvH4_2g23920
malus_domestica MD11G1308600.v1.1
prunus_persica Prupe.8G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291551
rosa_laevigata RLG00000012108
rosa_multiflora Rmu_sc0002226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175980
rosa_rugosa Rorug06G0220200
rosa_samantha Rh6AG332400 Rh6BG339300 Rh6CG345600 Rh6DG332900
rosa_wichuraiana Rw6G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1000
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 2 cut(s) 1000, 1460
AccB1I GGYRCC 1 cut(s) 832
AccII CGCG 4 cut(s) 219, 345, 353, 2948
AccIII TCCGGA 1 cut(s) 1426
AclWI GGATC 2 cut(s) 377, 1603
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 8 cut(s) 149, 415, 657, 902, 1493, 1922, 2251, 2935
AcuI CTGAAG 5 cut(s) 260, 1221, 1652, 1836, 2307
AcyI GRCGYC 2 cut(s) 222, 291
AdeI CACNNNGTG 2 cut(s) 315, 1738
AfaI GTAC 8 cut(s) 323, 514, 527, 918, 1032, 1450, 2224, 2763
AflIII ACRYGT 1 cut(s) 1307
AgeI ACCGGT 1 cut(s) 2279
AhdI GACNNNNNGTC 1 cut(s) 295
AjnI CCWGG 6 cut(s) 80, 835, 1252, 2419, 2722, 2939
AjuI GAANNNNNNNTTGG 2 cut(s) 2111, 2143
Alw21I GWGCWC 2 cut(s) 770, 1248
Alw26I GTCTC 6 cut(s) 62, 979, 1854, 2207, 2375, 2651
AlwI GGATC 2 cut(s) 377, 1603
AlwNI CAGNNNCTG 1 cut(s) 1211
Ama87I CYCGRG 1 cut(s) 2197
Aor13HI TCCGGA 1 cut(s) 1426
AoxI GGCC 5 cut(s) 280, 348, 579, 1010, 2854
ApeKI GCWGC 4 cut(s) 1205, 1916, 1936, 2889
ApoI RAATTY 8 cut(s) 149, 415, 657, 902, 1493, 1922, 2251, 2935
AsiGI ACCGGT 1 cut(s) 2279
Asp700I GAANNNNTTC 1 cut(s) 2007
AspLEI GCGC 5 cut(s) 127, 345, 1441, 2838, 2948
AspS9I GGNCC 6 cut(s) 47, 927, 1011, 1256, 1429, 2855
AsuC2I CCSGG 2 cut(s) 2198, 2199
AvaI CYCGRG 1 cut(s) 2197
AvaII GGWCC 4 cut(s) 47, 927, 1256, 1429
AxyI CCTNAGG 1 cut(s) 1860
BaeGI GKGCMC 1 cut(s) 835
BanI GGYRCC 1 cut(s) 832
BarI GAAGNNNNNNTAC 2 cut(s) 2215, 2247
BbsI GAAGAC 2 cut(s) 1658, 1858
Bbv12I GWGCWC 2 cut(s) 770, 1248
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 4 cut(s) 1217, 1903, 1923, 2901
BccI CCATC 6 cut(s) 1048, 1226, 2060, 2424, 2669, 2749
BciT130I CCWGG 6 cut(s) 82, 837, 1254, 2421, 2724, 2941
BciVI GTATCC 1 cut(s) 2420
BclI TGATCA 2 cut(s) 481, 1720
BcnI CCSGG 2 cut(s) 2198, 2199
BcoDI GTCTC 6 cut(s) 62, 979, 1854, 2207, 2375, 2651
BfaI CTAG 7 cut(s) 338, 557, 668, 1008, 2024, 2577, 2870
BfmI CTRYAG 4 cut(s) 1553, 1983, 2386, 2957
BfoI RGCGCY 2 cut(s) 128, 1442
BfuAI ACCTGC 2 cut(s) 1000, 1460
BfuI GTATCC 1 cut(s) 2420
BglII AGATCT 2 cut(s) 1193, 1798
BisI GCNGC 7 cut(s) 123, 351, 354, 1206, 1917, 1937, 2890
BlpI GCTNAGC 1 cut(s) 2523
BlsI GCNGC 7 cut(s) 124, 352, 355, 1207, 1918, 1938, 2891
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 8 cut(s) 82, 837, 1254, 2198, 2199, 2421, 2724, 2941
Bme18I GGWCC 4 cut(s) 47, 927, 1256, 1429
BmeRI GACNNNNNGTC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 2197
BmgT120I GGNCC 6 cut(s) 47, 927, 1011, 1256, 1429, 2855
BmiI GGNNCC 4 cut(s) 834, 928, 2857, 2876
BmrFI CCNGG 8 cut(s) 82, 837, 1254, 2198, 2199, 2421, 2724, 2941
BmsI GCATC 9 cut(s) 311, 484, 661, 667, 2122, 2237, 2470, 2703, 2932
BpiI GAAGAC 2 cut(s) 1658, 1858
BplI GAGNNNNNCTC 2 cut(s) 2334, 2366
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 2523
BpuMI CCSGG 2 cut(s) 2198, 2199
BsaHI GRCGYC 2 cut(s) 222, 291
BsaI GGTCTC 4 cut(s) 62, 2207, 2375, 2651
BsaWI WCCGGW 2 cut(s) 1426, 2279
Bse118I RCCGGY 1 cut(s) 2279
Bse1I ACTGG 2 cut(s) 208, 295
Bse21I CCTNAGG 1 cut(s) 1860
Bse3DI GCAATG 2 cut(s) 957, 2699
BseAI TCCGGA 1 cut(s) 1426
BseBI CCWGG 6 cut(s) 82, 837, 1254, 2421, 2724, 2941
BseGI GGATG 8 cut(s) 20, 302, 786, 1150, 1690, 2137, 2416, 2764
BseMI GCAATG 2 cut(s) 957, 2699
BseMII CTCAG 7 cut(s) 567, 1233, 1495, 1543, 1660, 1874, 2514
BseNI ACTGG 2 cut(s) 208, 295
BseRI GAGGAG 5 cut(s) 67, 448, 1349, 1802, 2595
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 4 cut(s) 1217, 1903, 1923, 2901
BseYI CCCAGC 3 cut(s) 356, 721, 2860
BsgI GTGCAG 1 cut(s) 1922
Bsh1236I CGCG 4 cut(s) 219, 345, 353, 2948
BshFI GGCC 5 cut(s) 282, 350, 581, 1012, 2856
BshNI GGYRCC 1 cut(s) 832
BshTI ACCGGT 1 cut(s) 2279
BsiHKAI GWGCWC 2 cut(s) 770, 1248
BsiHKCI CYCGRG 1 cut(s) 2197
BsiSI CCGG 3 cut(s) 1427, 2198, 2280
BslFI GGGAC 3 cut(s) 122, 1672, 2666
BsmAI GTCTC 6 cut(s) 62, 979, 1854, 2207, 2375, 2651
BsmFI GGGAC 3 cut(s) 122, 1672, 2666
BsmI GAATGC 1 cut(s) 990
BsnI GGCC 5 cut(s) 282, 350, 581, 1012, 2856
Bso31I GGTCTC 4 cut(s) 62, 2207, 2375, 2651
BsoBI CYCGRG 1 cut(s) 2197
Bsp1286I GDGCHC 3 cut(s) 770, 835, 1248
Bsp13I TCCGGA 1 cut(s) 1426
Bsp143I GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
Bsp1720I GCTNAGC 1 cut(s) 2523
Bsp19I CCATGG 1 cut(s) 1654
BspANI GGCC 5 cut(s) 282, 350, 581, 1012, 2856
BspCNI CTCAG 7 cut(s) 568, 1234, 1494, 1542, 1659, 1873, 2515
BspEI TCCGGA 1 cut(s) 1426
BspFNI CGCG 4 cut(s) 219, 345, 353, 2948
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 4 cut(s) 834, 928, 2857, 2876
BspMAI CTGCAG 1 cut(s) 1557
BspMI ACCTGC 2 cut(s) 1000, 1460
BspPI GGATC 2 cut(s) 377, 1603
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 4 cut(s) 62, 2207, 2375, 2651
BsrDI GCAATG 2 cut(s) 957, 2699
BsrFI RCCGGY 1 cut(s) 2279
BsrI ACTGG 2 cut(s) 208, 295
BssAI RCCGGY 1 cut(s) 2279
BssMI GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
BssNI GRCGYC 2 cut(s) 222, 291
BssT1I CCWWGG 4 cut(s) 390, 1654, 2151, 2639
Bst2UI CCWGG 6 cut(s) 82, 837, 1254, 2421, 2724, 2941
Bst4CI ACNGT 7 cut(s) 56, 178, 1374, 1518, 1539, 1995, 2374
Bst6I CTCTTC 5 cut(s) 69, 594, 611, 743, 2614
BstACI GRCGYC 2 cut(s) 222, 291
BstC8I GCNNGC 2 cut(s) 2060, 2401
BstDSI CCRYGG 2 cut(s) 351, 1654
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstF5I GGATG 8 cut(s) 20, 302, 786, 1150, 1690, 2137, 2416, 2764
BstFNI CGCG 4 cut(s) 219, 345, 353, 2948
BstH2I RGCGCY 2 cut(s) 128, 1442
BstHHI GCGC 5 cut(s) 127, 345, 1441, 2838, 2948
BstKTI GATC 7 cut(s) 372, 484, 1196, 1598, 1723, 1801, 1978
BstMAI GTCTC 6 cut(s) 62, 979, 1854, 2207, 2375, 2651
BstMBI GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
BstMWI GCNNNNNNNGC 3 cut(s) 857, 1283, 1925
BstNI CCWGG 6 cut(s) 82, 837, 1254, 2421, 2724, 2941
BstNSI RCATGY 3 cut(s) 1126, 1311, 1528
BstSCI CCNGG 8 cut(s) 80, 835, 1252, 2196, 2197, 2419, 2722, 2939
BstSFI CTRYAG 4 cut(s) 1553, 1983, 2386, 2957
BstSLI GKGCMC 1 cut(s) 835
BstUI CGCG 4 cut(s) 219, 345, 353, 2948
BstV1I GCAGC 4 cut(s) 1217, 1903, 1923, 2901
BstV2I GAAGAC 2 cut(s) 1658, 1858
BstX2I RGATCY 2 cut(s) 1193, 1798
BstXI CCANNNNNNTGG 1 cut(s) 2554
BstYI RGATCY 2 cut(s) 1193, 1798
Bsu36I CCTNAGG 1 cut(s) 1860
BsuI GTATCC 1 cut(s) 2420
BsuRI GGCC 5 cut(s) 282, 350, 581, 1012, 2856
BtgI CCRYGG 2 cut(s) 351, 1654
BtsCI GGATG 8 cut(s) 20, 302, 786, 1150, 1690, 2137, 2416, 2764
BtsI GCAGTG 1 cut(s) 1837
BtsIMutI CAGTG 5 cut(s) 1514, 1654, 1837, 2000, 2370
BveI ACCTGC 2 cut(s) 1000, 1460
Cac8I GCNNGC 2 cut(s) 2060, 2401
CaiI CAGNNNCTG 1 cut(s) 1211
CciI TCATGA 1 cut(s) 484
CfoI GCGC 5 cut(s) 127, 345, 1441, 2838, 2948
Cfr10I RCCGGY 1 cut(s) 2279
Cfr13I GGNCC 6 cut(s) 47, 927, 1011, 1256, 1429, 2855
Cfr42I CCGCGG 1 cut(s) 354
Cfr9I CCCGGG 1 cut(s) 2197
CseI GACGC 2 cut(s) 230, 2516
Csp6I GTAC 8 cut(s) 322, 513, 526, 917, 1031, 1449, 2223, 2762
CspAI ACCGGT 1 cut(s) 2279
CspCI CAANNNNNGTGG 6 cut(s) 487, 522, 1753, 1788, 2275, 2310
CviQI GTAC 8 cut(s) 322, 513, 526, 917, 1031, 1449, 2223, 2762
DpnI GATC 7 cut(s) 371, 483, 1195, 1597, 1722, 1800, 1977
DpnII GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 2 cut(s) 315, 1738
DriI GACNNNNNGTC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 5 cut(s) 69, 594, 611, 743, 2614
Eam1105I GACNNNNNGTC 1 cut(s) 295
EarI CTCTTC 5 cut(s) 69, 594, 611, 743, 2614
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 4 cut(s) 390, 1654, 2151, 2639
Eco31I GGTCTC 4 cut(s) 62, 2207, 2375, 2651
Eco47I GGWCC 4 cut(s) 47, 927, 1256, 1429
Eco57I CTGAAG 5 cut(s) 260, 1221, 1652, 1836, 2307
Eco81I CCTNAGG 1 cut(s) 1860
Eco88I CYCGRG 1 cut(s) 2197
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoRII CCWGG 6 cut(s) 80, 835, 1252, 2419, 2722, 2939
EcoT14I CCWWGG 4 cut(s) 390, 1654, 2151, 2639
EcoT22I ATGCAT 1 cut(s) 1124
ErhI CCWWGG 4 cut(s) 390, 1654, 2151, 2639
FalI AAGNNNNNCTT 4 cut(s) 903, 935, 1701, 1733
FaqI GGGAC 3 cut(s) 122, 1672, 2666
FauI CCCGC 2 cut(s) 148, 673
FauNDI CATATG 1 cut(s) 1050
FbaI TGATCA 2 cut(s) 481, 1720
Fnu4HI GCNGC 7 cut(s) 123, 351, 354, 1206, 1917, 1937, 2890
FokI GGATG 8 cut(s) 7, 289, 793, 1157, 1697, 2144, 2403, 2771
Fsp4HI GCNGC 7 cut(s) 123, 351, 354, 1206, 1917, 1937, 2890
FspBI CTAG 7 cut(s) 338, 557, 668, 1008, 2024, 2577, 2870
GlaI GCGC 5 cut(s) 126, 344, 1440, 2837, 2947
GluI GCNGC 7 cut(s) 123, 351, 354, 1206, 1917, 1937, 2890
GsaI CCCAGC 3 cut(s) 360, 725, 2864
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 2 cut(s) 128, 1442
HaeIII GGCC 5 cut(s) 282, 350, 581, 1012, 2856
HapII CCGG 3 cut(s) 1427, 2198, 2280
HgaI GACGC 2 cut(s) 230, 2516
HhaI GCGC 5 cut(s) 127, 345, 1441, 2838, 2948
Hin1I GRCGYC 2 cut(s) 222, 291
Hin6I GCGC 5 cut(s) 125, 343, 1439, 2836, 2946
HinP1I GCGC 5 cut(s) 125, 343, 1439, 2836, 2946
HincII GTYRAC 2 cut(s) 383, 1746
HindII GTYRAC 2 cut(s) 383, 1746
HinfI GANTC 8 cut(s) 5, 379, 854, 878, 1901, 2332, 2393, 2767
HpaII CCGG 3 cut(s) 1427, 2198, 2280
Hpy166II GTNNAC 7 cut(s) 383, 526, 1031, 1535, 1746, 1841, 2003
Hpy188III TCNNGA 8 cut(s) 485, 882, 981, 1133, 1427, 1670, 2183, 2827
Hpy8I GTNNAC 7 cut(s) 383, 526, 1031, 1535, 1746, 1841, 2003
Hpy99I CGWCG 4 cut(s) 224, 251, 293, 2184
HpyCH4III ACNGT 7 cut(s) 56, 178, 1374, 1518, 1539, 1995, 2374
HpyCH4IV ACGT 3 cut(s) 291, 844, 1150
HpyF10VI GCNNNNNNNGC 3 cut(s) 857, 1283, 1925
HpySE526I ACGT 3 cut(s) 291, 844, 1150
Hsp92I GRCGYC 2 cut(s) 222, 291
HspAI GCGC 5 cut(s) 125, 343, 1439, 2836, 2946
Kpn2I TCCGGA 1 cut(s) 1426
Ksp22I TGATCA 2 cut(s) 481, 1720
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
LmnI GCTCC 6 cut(s) 394, 773, 1135, 1554, 1775, 2943
Lsp1109I GCAGC 4 cut(s) 1217, 1903, 1923, 2901
LweI GCATC 9 cut(s) 311, 484, 661, 667, 2122, 2237, 2470, 2703, 2932
MaeI CTAG 7 cut(s) 338, 557, 668, 1008, 2024, 2577, 2870
MaeII ACGT 3 cut(s) 291, 844, 1150
MaeIII GTNAC 8 cut(s) 889, 1237, 1520, 1628, 1649, 1884, 2282, 2491
MalI GATC 7 cut(s) 371, 483, 1195, 1597, 1722, 1800, 1977
MboI GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
MfeI CAATTG 1 cut(s) 2012
MflI RGATCY 2 cut(s) 1193, 1798
MhlI GDGCHC 3 cut(s) 770, 835, 1248
MlyI GAGTC 2 cut(s) 388, 887
MmeI TCCRAC 4 cut(s) 130, 330, 1035, 1919
Mph1103I ATGCAT 1 cut(s) 1124
MroI TCCGGA 1 cut(s) 1426
MroXI GAANNNNTTC 1 cut(s) 2007
MslI CAYNNNNRTG 4 cut(s) 489, 2130, 2295, 2820
MspA1I CMGCKG 3 cut(s) 353, 2399, 2550
MspI CCGG 3 cut(s) 1427, 2198, 2280
MspR9I CCNGG 8 cut(s) 82, 837, 1254, 2198, 2199, 2421, 2724, 2941
MunI CAATTG 1 cut(s) 2012
Mva1269I GAATGC 1 cut(s) 990
MvaI CCWGG 6 cut(s) 82, 837, 1254, 2421, 2724, 2941
MvnI CGCG 4 cut(s) 219, 345, 353, 2948
MwoI GCNNNNNNNGC 3 cut(s) 857, 1283, 1925
NciI CCSGG 2 cut(s) 2198, 2199
NcoI CCATGG 1 cut(s) 1654
NdeI CATATG 1 cut(s) 1050
NdeII GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
NlaIV GGNNCC 4 cut(s) 834, 928, 2857, 2876
NmeAIII GCCGAG 2 cut(s) 250, 258
NmuCI GTSAC 5 cut(s) 1237, 1649, 1884, 2282, 2491
NsiI ATGCAT 1 cut(s) 1124
NspI RCATGY 3 cut(s) 1126, 1311, 1528
PagI TCATGA 1 cut(s) 484
PaqCI CACCTGC 1 cut(s) 1000
PasI CCCWGGG 1 cut(s) 2723
PciI ACATGT 1 cut(s) 1307
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 990
PdmI GAANNNNTTC 1 cut(s) 2007
PfeI GAWTC 5 cut(s) 854, 1901, 2332, 2393, 2767
PfoI TCCNGGA 2 cut(s) 2419, 2939
PinAI ACCGGT 1 cut(s) 2279
PkrI GCNGC 7 cut(s) 124, 352, 355, 1207, 1918, 1938, 2891
PleI GAGTC 2 cut(s) 387, 886
PpsI GAGTC 2 cut(s) 387, 886
PscI ACATGT 1 cut(s) 1307
Psp6I CCWGG 6 cut(s) 80, 835, 1252, 2419, 2722, 2939
PspFI CCCAGC 3 cut(s) 356, 721, 2860
PspGI CCWGG 6 cut(s) 80, 835, 1252, 2419, 2722, 2939
PspN4I GGNNCC 4 cut(s) 834, 928, 2857, 2876
PspPI GGNCC 6 cut(s) 47, 927, 1011, 1256, 1429, 2855
PstI CTGCAG 1 cut(s) 1557
PstNI CAGNNNCTG 1 cut(s) 1211
PsuI RGATCY 2 cut(s) 1193, 1798
PvuII CAGCTG 1 cut(s) 2399
RsaI GTAC 8 cut(s) 323, 514, 527, 918, 1032, 1450, 2224, 2763
RsaNI GTAC 8 cut(s) 322, 513, 526, 917, 1031, 1449, 2223, 2762
RseI CAYNNNNRTG 4 cut(s) 489, 2130, 2295, 2820
SacII CCGCGG 1 cut(s) 354
SatI GCNGC 7 cut(s) 123, 351, 354, 1206, 1917, 1937, 2890
Sau3AI GATC 7 cut(s) 369, 481, 1193, 1595, 1720, 1798, 1975
Sau96I GGNCC 6 cut(s) 47, 927, 1011, 1256, 1429, 2855
ScaI AGTACT 1 cut(s) 514
SchI GAGTC 2 cut(s) 388, 887
ScrFI CCNGG 8 cut(s) 82, 837, 1254, 2198, 2199, 2421, 2724, 2941
SduI GDGCHC 3 cut(s) 770, 835, 1248
SfaNI GCATC 9 cut(s) 311, 484, 661, 667, 2122, 2237, 2470, 2703, 2932
SfcI CTRYAG 4 cut(s) 1553, 1983, 2386, 2957
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
SinI GGWCC 4 cut(s) 47, 927, 1256, 1429
SmaI CCCGGG 1 cut(s) 2199
SmiMI CAYNNNNRTG 4 cut(s) 489, 2130, 2295, 2820
SspI AATATT 2 cut(s) 1705, 1876
SspMI CTAG 7 cut(s) 338, 557, 668, 1008, 2024, 2577, 2870
StyD4I CCNGG 8 cut(s) 80, 835, 1252, 2196, 2197, 2419, 2722, 2939
StyI CCWWGG 4 cut(s) 390, 1654, 2151, 2639
TaaI ACNGT 7 cut(s) 56, 178, 1374, 1518, 1539, 1995, 2374
TaiI ACGT 3 cut(s) 294, 847, 1153
TaqI TCGA 5 cut(s) 8, 823, 2182, 2849, 2903
TatI WGTACW 5 cut(s) 512, 525, 916, 1030, 2761
TauI GCSGC 3 cut(s) 125, 353, 356
TfiI GAWTC 5 cut(s) 854, 1901, 2332, 2393, 2767
TscAI CASTG 5 cut(s) 1521, 1654, 1837, 2000, 2377
TseFI GTSAC 5 cut(s) 1237, 1649, 1884, 2282, 2491
TseI GCWGC 4 cut(s) 1205, 1916, 1936, 2889
Tsp45I GTSAC 5 cut(s) 1237, 1649, 1884, 2282, 2491
TspGWI ACGGA 3 cut(s) 124, 919, 2520
TspMI CCCGGG 1 cut(s) 2197
TspRI CASTG 5 cut(s) 1521, 1654, 1837, 2000, 2377
VpaK11BI GGWCC 4 cut(s) 47, 927, 1256, 1429
XagI CCTNNNNNAGG 1 cut(s) 1137
XapI RAATTY 8 cut(s) 149, 415, 657, 902, 1493, 1922, 2251, 2935
XceI RCATGY 3 cut(s) 1126, 1311, 1528
XcmI CCANNNNNNNNNTGG 1 cut(s) 2637
XmaI CCCGGG 1 cut(s) 2197
XmnI GAANNNNTTC 1 cut(s) 2007
XspI CTAG 7 cut(s) 338, 557, 668, 1008, 2024, 2577, 2870
ZraI GACGTC 1 cut(s) 292
ZrmI AGTACT 1 cut(s) 514
Zsp2I ATGCAT 1 cut(s) 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.