RLG00000012108

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016760
Physical Location & Seq
Reverse (-)
15313972 .. 15318051
4080 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012108

Sequence Viewer

Length: 2775 bp
ATGGACTCGATTTTAAACTCATCCCTGCAAGAAATCTGCTCGGAAGGTCCAAACGGTCTCCCTCTTCAAACCCTCTCCTCCAGACTCAACCTCTCTCCGCCGCTCCAACAACCCCTATGGGCGGCGCTACTCTCCGTCCCCGCCCTCAAATTCCACACCCAAACCCAAAACGCCACCGTTTCGCACCTCCCCACAGACCCTTCAATCCAGTCGTTCCGCGACGCCGAGAAGCTCAACCTGAAGCTCGTCGCCGACCAGCCTCTCCGCAACAACTTCCTCGGCCTGTACGACGTCCAGTCCGCATCCGACACCATGTGCGAGTACCAACGCAAAACCCTAGAGCGCGTGGCCGCGGCTGGGTCAAAGGGGATCACGCAGAGTCAACTATCCAAGGAGCTTGGGATTAGAGGTGAGAATTTCTTTTACAAATTGAGGAGGCTCGAATGTCAGGGCTTGATTGTGAAGCAACCATTGAATGATGATCATGATGATGATGCCAATGTGAGTGGGAGTACTAATTTAGTGTACTTAAATCGTTATGCAAAGCATTTAGGTCTAGGGTCTGAACAAAAAGCTGAGGCCACTAAATCTAAGGAGGATGAGAATATGACTAAGAAGAGTGGTAATTTGGTAGCGGAACTTCCGATTGAGCATCAAATTTATGAGCTGGTTAATGATGCGGGGTCAAGGGGGTTCATGGTGTTTAGGGTTGCAGAGAGGCTGGGAATTGTTAGGAAAGAGAATAGGAAGAGGATTGAAAGTTTGTGCTCCAAGTTTGGGATGATAATGGATAAAGAGAAATGCGGCAAGACCAAAGTGTATCGAGTTTGGGCACCTGGGAAACGTAATGCTGAATCAGCTAATGCAATTCTGAATGAGTCTGAAAATGTTACTCAAAGTAAATTTAATGAAGATGTTCTTCGTGGGTCCGTTCAGAAAGGTGATACAGCAATAAATGATGTCTCATCAGGAATGCCACCTGCTGTGGAATGTGCTAGGCCCAGAAGGGGAAAAAGTGTACTTGAACCATCACAGGTTAAAGGGGATGAACGTCAATCTCTGGGAATTAGAAGCCCTTTGCAGAAAAGAAAGAGATCTTCTGAAGCAGCTTCTGTAAAGTTTATAGAAGTTGATGGCGTAACTGAGCACCAACCTGGACCAAATGAAGGTGGTGAGGCGAGGTGTGCTTTTTCAAAGTCAAAATCAATTTGTCAAAGAAGGTTTTTATGGACAGAGGAGGCTGATAGGCAATTAGTTATCCAATATGTAAGATGGTGTGCAACTGTTGGGGCAAAATATCATCACCAGATAAACTGGGCTTCACTTCCGGACCTTCCAGCGCCTCCAAGTACTTGCAAAAGAAGAATGTCATATCTGAAACTCAGTAATGGAAAATGTAGAAAATCTTTAATGACACTGTGTAACATGCTCAGTAAACGGTATGCAAAGCTCCTGCAGAAAACCCAGAACAGGTCATTTTACCAAGATGATTGCGGATCACTTTTGCAAGGTTCAACAGAGGAGGGTTGTAACCAGAATTATCCTCTCAGTGACCGTGGGACAGGTCTTCAGGAAGAACAATGGGATGACTTTAATGACAAAAATATTAAAGAAGTGCTTGATCAGATACTTCACTATGTGAGGTTGACTAAATCTGTTGCCTCCAAAAGATGTGGAGCTTCCTGCGAGGAGGGTTCAGATCTTAATGTTAATGCTGAAGATGTTGAAGGCAGTGTTTCTGAACCTTTGTCTCTCTCACCTCAGGTCTTCTCCAATATTTGCAAGTCACCATTTCCAACTGATTCTGGCAAGAGAGCTGCCAAATTTGCTCATTGGCTCCACAAAAGAGATAGAGATCTTATGGGAAGGGGGCTTGATCTTCCTACAGATATACAGTGTGGTGAACTTTTTCATTTGTTTGCTCTAGTTTTTTCAGGTGAATTGTCAATCTCCCCATGCTTGCCAGATGGAGTGGGAGAAGCAGAAGATTTGAGAAGCTCAAAACATAAAATTGGTAGGAATAAACATTTGGATGCTAAAAGACTAAAATCCTTGGTTGCAGAAAGAGAGATTATTTCCCGTCGAGAAAAAGGTTTCCCGGGTCTCATGGTATCTGTATGTAGTACAGCATCTTCTAAAGCCTATTGTGAAAATTTGTTCAAAGATGATGATACTCTTACTGGTGACAAACGTTGTGGTGAAAATTATCAGCAAGACAGTACTTCAGGTATGAATCAGATTAGAGGAAATCTCAATTCAGGCATTGTTATCACTGTGGCAGAGACCTGTAGTGAATCAGCTGGCGAAGGTATCCTCCATCCTGGAAATTGTGATATTGGTGGAACTCTTTTGATAGGGAAAACAAATATGAATGTTAATGATGCACATAATGTGACTATTCTTAATTCTCCTAAAGAGGTTGCTGAGCGTCTGTCTGAAAAACTAACCAGCGGTGTGGAAGGCAAAGTTTTCTCACCTATAGGAGATTTTGATGAATGTTCTAAACTCTCTTCTGATGAATTATGTTTGCCACTTCTTCCTTGGATAAATGGGGATGGGACCATCAACAAGACTATCTATGAGGGGTTCATGCAACGTGTTTTTGGGATTGTGATGCAAAACCCCGGGATATTAGAGGGTGAAATTATCCGTAAGATGGATGTACTGAGTCCACAGAGTTGTAGAAAATTGTTGGCGGAGATGATTGAAGCAAAACATCTTCATGTCAGGACAATGCGCCAAGCCACATCGAATGCCCCCCCCCGGCATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

925

Amino Acids

102.53

Weight (kDa)

8.22

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 96 4.9e-25 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 108 - 182 4.4e-14 B-block binding subunit of TFIIIC
DUF7646 PF24657 209 - 285 1.4e-26 Domain of unknown function (DUF7646)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23920 FvH4_2g23920
malus_domestica MD11G1308600.v1.1
prunus_persica Prupe.8G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291551
rosa_laevigata RLG00000012108
rosa_multiflora Rmu_sc0002226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175980
rosa_rugosa Rorug06G0220200
rosa_samantha Rh6AG332400 Rh6BG339300 Rh6CG345600 Rh6DG332900
rosa_wichuraiana Rw6G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 988
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 988
AccB1I GGYRCC 1 cut(s) 832
AccBSI CCGCTC 1 cut(s) 103
AccII CGCG 3 cut(s) 219, 345, 353
AccIII TCCGGA 1 cut(s) 1327
AclI AACGTT 1 cut(s) 2191
AclWI GGATC 2 cut(s) 377, 1504
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 6 cut(s) 149, 415, 657, 902, 1823, 2152
AcuI CTGAAG 5 cut(s) 260, 1122, 1553, 1737, 2208
AcyI GRCGYC 2 cut(s) 222, 291
AdeI CACNNNGTG 2 cut(s) 315, 1639
AfaI GTAC 9 cut(s) 287, 323, 514, 527, 1020, 1351, 2125, 2221, 2664
AfiI CCNNNNNNNGG 8 cut(s) 121, 357, 777, 1007, 1166, 1470, 2656, 2763
AflIII ACRYGT 1 cut(s) 2596
AhdI GACNNNNNGTC 1 cut(s) 295
AjnI CCWGG 3 cut(s) 835, 1153, 2320
AjuI GAANNNNNNNTTGG 2 cut(s) 2012, 2044
Alw21I GWGCWC 2 cut(s) 770, 1149
Alw26I GTCTC 5 cut(s) 62, 967, 1755, 2108, 2276
AlwI GGATC 2 cut(s) 377, 1504
AlwNI CAGNNNCTG 1 cut(s) 1112
Ama87I CYCGRG 2 cut(s) 2098, 2624
Aor13HI TCCGGA 1 cut(s) 1327
AoxI GGCC 4 cut(s) 280, 348, 579, 998
ApeKI GCWGC 2 cut(s) 1106, 1817
ApoI RAATTY 6 cut(s) 149, 415, 657, 902, 1823, 2152
Asp700I GAANNNNTTC 2 cut(s) 915, 1908
AspLEI GCGC 4 cut(s) 127, 345, 1342, 2739
AspS9I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2559
AsuC2I CCSGG 5 cut(s) 2099, 2100, 2625, 2626, 2764
AvaI CYCGRG 2 cut(s) 2098, 2624
AvaII GGWCC 5 cut(s) 47, 927, 1157, 1330, 2559
AxyI CCTNAGG 1 cut(s) 1761
BaeGI GKGCMC 1 cut(s) 835
BanI GGYRCC 1 cut(s) 832
BarI GAAGNNNNNNTAC 4 cut(s) 624, 656, 2116, 2148
BbsI GAAGAC 2 cut(s) 1559, 1759
Bbv12I GWGCWC 2 cut(s) 770, 1149
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 2 cut(s) 1118, 1804
BccI CCATC 8 cut(s) 1036, 1127, 1266, 1961, 2325, 2549, 2570, 2650
BciT130I CCWGG 3 cut(s) 837, 1155, 2322
BciVI GTATCC 1 cut(s) 2321
BclI TGATCA 2 cut(s) 481, 1621
BcnI CCSGG 5 cut(s) 2099, 2100, 2625, 2626, 2764
BcoDI GTCTC 5 cut(s) 62, 967, 1755, 2108, 2276
BfaI CTAG 5 cut(s) 338, 557, 996, 1925, 2773
BfmI CTRYAG 4 cut(s) 1454, 1884, 2287, 2478
BfoI RGCGCY 2 cut(s) 128, 1343
BfuAI ACCTGC 1 cut(s) 988
BfuI GTATCC 1 cut(s) 2321
BglII AGATCT 3 cut(s) 1094, 1699, 1855
BisI GCNGC 7 cut(s) 101, 123, 351, 354, 805, 1107, 1818
BlpI GCTNAGC 1 cut(s) 2424
BlsI GCNGC 7 cut(s) 102, 124, 352, 355, 806, 1108, 1819
BmcAI AGTACT 3 cut(s) 514, 1351, 2221
Bme1390I CCNGG 8 cut(s) 837, 1155, 2099, 2100, 2322, 2625, 2626, 2764
Bme18I GGWCC 5 cut(s) 47, 927, 1157, 1330, 2559
BmeRI GACNNNNNGTC 1 cut(s) 295
BmeT110I CYCGRG 2 cut(s) 2098, 2624
BmgT120I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2559
BmiI GGNNCC 4 cut(s) 834, 928, 1838, 2560
BmrFI CCNGG 8 cut(s) 837, 1155, 2099, 2100, 2322, 2625, 2626, 2764
BmrI ACTGGG 1 cut(s) 1324
BmsI GCATC 8 cut(s) 311, 484, 661, 667, 2023, 2138, 2371, 2604
BmuI ACTGGG 1 cut(s) 1324
BpiI GAAGAC 2 cut(s) 1559, 1759
BplI GAGNNNNNCTC 2 cut(s) 2235, 2267
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 2424
BpuMI CCSGG 5 cut(s) 2099, 2100, 2625, 2626, 2764
BsaBI GATNNNNATC 1 cut(s) 1854
BsaHI GRCGYC 2 cut(s) 222, 291
BsaI GGTCTC 3 cut(s) 62, 2108, 2276
BsaWI WCCGGW 1 cut(s) 1327
Bsc4I CCNNNNNNNGG 8 cut(s) 121, 357, 777, 1007, 1166, 1470, 2656, 2763
Bse1I ACTGG 4 cut(s) 208, 295, 1319, 2185
Bse21I CCTNAGG 1 cut(s) 1761
Bse8I GATNNNNATC 1 cut(s) 1854
BseAI TCCGGA 1 cut(s) 1327
BseBI CCWGG 3 cut(s) 837, 1155, 2322
BseJI GATNNNNATC 1 cut(s) 1854
BseLI CCNNNNNNNGG 8 cut(s) 121, 357, 777, 1007, 1166, 1470, 2656, 2763
BseMII CTCAG 8 cut(s) 567, 1134, 1396, 1444, 1561, 1775, 2415, 2657
BseNI ACTGG 4 cut(s) 208, 295, 1319, 2185
BseRI GAGGAG 5 cut(s) 67, 448, 1250, 1535, 1703
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 2 cut(s) 1118, 1804
BseYI CCCAGC 2 cut(s) 356, 721
Bsh1236I CGCG 3 cut(s) 219, 345, 353
BshFI GGCC 4 cut(s) 282, 350, 581, 1000
BshNI GGYRCC 1 cut(s) 832
BsiHKAI GWGCWC 2 cut(s) 770, 1149
BsiHKCI CYCGRG 2 cut(s) 2098, 2624
BsiSI CCGG 4 cut(s) 1328, 2099, 2625, 2764
BslFI GGGAC 3 cut(s) 122, 1573, 2572
BslI CCNNNNNNNGG 8 cut(s) 121, 357, 777, 1007, 1166, 1470, 2656, 2763
BsmAI GTCTC 5 cut(s) 62, 967, 1755, 2108, 2276
BsmFI GGGAC 3 cut(s) 122, 1573, 2572
BsmI GAATGC 2 cut(s) 978, 2758
BsnI GGCC 4 cut(s) 282, 350, 581, 1000
Bso31I GGTCTC 3 cut(s) 62, 2108, 2276
BsoBI CYCGRG 2 cut(s) 2098, 2624
Bsp1286I GDGCHC 3 cut(s) 770, 835, 1149
Bsp13I TCCGGA 1 cut(s) 1327
Bsp143I GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
Bsp1720I GCTNAGC 1 cut(s) 2424
BspANI GGCC 4 cut(s) 282, 350, 581, 1000
BspCNI CTCAG 8 cut(s) 568, 1135, 1395, 1443, 1560, 1774, 2416, 2658
BspEI TCCGGA 1 cut(s) 1327
BspFNI CGCG 3 cut(s) 219, 345, 353
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 4 cut(s) 834, 928, 1838, 2560
BspMAI CTGCAG 1 cut(s) 1458
BspMI ACCTGC 1 cut(s) 988
BspPI GGATC 2 cut(s) 377, 1504
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 3 cut(s) 62, 2108, 2276
BsrBI CCGCTC 1 cut(s) 103
BsrI ACTGG 4 cut(s) 208, 295, 1319, 2185
BssMI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
BssNI GRCGYC 2 cut(s) 222, 291
BssT1I CCWWGG 3 cut(s) 390, 2052, 2540
Bst2UI CCWGG 3 cut(s) 837, 1155, 2322
Bst4CI ACNGT 9 cut(s) 56, 178, 1285, 1419, 1440, 1556, 1896, 2219, 2275
Bst6I CTCTTC 4 cut(s) 69, 611, 743, 2515
BstACI GRCGYC 2 cut(s) 222, 291
BstC8I GCNNGC 2 cut(s) 1961, 2302
BstDSI CCRYGG 2 cut(s) 351, 1555
BstFNI CGCG 3 cut(s) 219, 345, 353
BstH2I RGCGCY 2 cut(s) 128, 1343
BstHHI GCGC 4 cut(s) 127, 345, 1342, 2739
BstKTI GATC 8 cut(s) 372, 484, 1097, 1499, 1624, 1702, 1858, 1879
BstMAI GTCTC 5 cut(s) 62, 967, 1755, 2108, 2276
BstMBI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
BstMWI GCNNNNNNNGC 3 cut(s) 857, 1184, 1826
BstNI CCWGG 3 cut(s) 837, 1155, 2322
BstNSI RCATGY 1 cut(s) 1429
BstSCI CCNGG 8 cut(s) 835, 1153, 2097, 2098, 2320, 2623, 2624, 2762
BstSFI CTRYAG 4 cut(s) 1454, 1884, 2287, 2478
BstSLI GKGCMC 1 cut(s) 835
BstUI CGCG 3 cut(s) 219, 345, 353
BstV1I GCAGC 2 cut(s) 1118, 1804
BstV2I GAAGAC 2 cut(s) 1559, 1759
BstX2I RGATCY 3 cut(s) 1094, 1699, 1855
BstXI CCANNNNNNTGG 1 cut(s) 2455
BstYI RGATCY 3 cut(s) 1094, 1699, 1855
Bsu36I CCTNAGG 1 cut(s) 1761
BsuI GTATCC 1 cut(s) 2321
BsuRI GGCC 4 cut(s) 282, 350, 581, 1000
BtgI CCRYGG 2 cut(s) 351, 1555
BtsI GCAGTG 1 cut(s) 1738
BtsIMutI CAGTG 5 cut(s) 1415, 1555, 1738, 1901, 2271
BveI ACCTGC 1 cut(s) 988
Cac8I GCNNGC 2 cut(s) 1961, 2302
CaiI CAGNNNCTG 1 cut(s) 1112
CciI TCATGA 1 cut(s) 484
CfoI GCGC 4 cut(s) 127, 345, 1342, 2739
Cfr13I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2559
Cfr42I CCGCGG 1 cut(s) 354
Cfr9I CCCGGG 2 cut(s) 2098, 2624
CseI GACGC 2 cut(s) 230, 2417
Csp6I GTAC 9 cut(s) 286, 322, 513, 526, 1019, 1350, 2124, 2220, 2663
CspCI CAANNNNNGTGG 6 cut(s) 487, 522, 1654, 1689, 2176, 2211
CviAII CATG 8 cut(s) 313, 485, 697, 1426, 1956, 2107, 2590, 2723
CviQI GTAC 9 cut(s) 286, 322, 513, 526, 1019, 1350, 2124, 2220, 2663
DpnI GATC 8 cut(s) 371, 483, 1096, 1498, 1623, 1701, 1857, 1878
DpnII GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 2 cut(s) 315, 1639
DriI GACNNNNNGTC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 4 cut(s) 69, 611, 743, 2515
Eam1105I GACNNNNNGTC 1 cut(s) 295
EarI CTCTTC 4 cut(s) 69, 611, 743, 2515
EciI GGCGGA 2 cut(s) 87, 2711
Eco130I CCWWGG 3 cut(s) 390, 2052, 2540
Eco31I GGTCTC 3 cut(s) 62, 2108, 2276
Eco47I GGWCC 5 cut(s) 47, 927, 1157, 1330, 2559
Eco57I CTGAAG 5 cut(s) 260, 1122, 1553, 1737, 2208
Eco81I CCTNAGG 1 cut(s) 1761
Eco88I CYCGRG 2 cut(s) 2098, 2624
EcoRII CCWGG 3 cut(s) 835, 1153, 2320
EcoT14I CCWWGG 3 cut(s) 390, 2052, 2540
ErhI CCWWGG 3 cut(s) 390, 2052, 2540
FaeI CATG 8 cut(s) 316, 488, 700, 1429, 1959, 2110, 2593, 2726
FalI AAGNNNNNCTT 4 cut(s) 903, 935, 1602, 1634
FaqI GGGAC 3 cut(s) 122, 1573, 2572
FatI CATG 8 cut(s) 312, 484, 696, 1425, 1955, 2106, 2589, 2722
FauI CCCGC 2 cut(s) 148, 673
FbaI TGATCA 2 cut(s) 481, 1621
Fnu4HI GCNGC 7 cut(s) 101, 123, 351, 354, 805, 1107, 1818
Fsp4HI GCNGC 7 cut(s) 101, 123, 351, 354, 805, 1107, 1818
FspBI CTAG 5 cut(s) 338, 557, 996, 1925, 2773
GlaI GCGC 4 cut(s) 126, 344, 1341, 2738
GluI GCNGC 7 cut(s) 101, 123, 351, 354, 805, 1107, 1818
GsaI CCCAGC 2 cut(s) 360, 725
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 2 cut(s) 128, 1343
HaeIII GGCC 4 cut(s) 282, 350, 581, 1000
HapII CCGG 4 cut(s) 1328, 2099, 2625, 2764
HgaI GACGC 2 cut(s) 230, 2417
HhaI GCGC 4 cut(s) 127, 345, 1342, 2739
Hin1I GRCGYC 2 cut(s) 222, 291
Hin1II CATG 8 cut(s) 316, 488, 700, 1429, 1959, 2110, 2593, 2726
Hin6I GCGC 4 cut(s) 125, 343, 1340, 2737
HinP1I GCGC 4 cut(s) 125, 343, 1340, 2737
HincII GTYRAC 2 cut(s) 383, 1647
HindII GTYRAC 2 cut(s) 383, 1647
HinfI GANTC 9 cut(s) 5, 84, 379, 854, 878, 1802, 2233, 2294, 2668
HpaII CCGG 4 cut(s) 1328, 2099, 2625, 2764
Hpy166II GTNNAC 7 cut(s) 383, 526, 1019, 1436, 1647, 1904, 2672
Hpy188III TCNNGA 7 cut(s) 81, 485, 969, 1328, 1571, 2084, 2728
Hpy8I GTNNAC 7 cut(s) 383, 526, 1019, 1436, 1647, 1904, 2672
Hpy99I CGWCG 4 cut(s) 224, 251, 293, 2085
HpyCH4III ACNGT 9 cut(s) 56, 178, 1285, 1419, 1440, 1556, 1896, 2219, 2275
HpyCH4IV ACGT 5 cut(s) 291, 844, 1051, 2191, 2596
HpyF10VI GCNNNNNNNGC 3 cut(s) 857, 1184, 1826
HpySE526I ACGT 5 cut(s) 291, 844, 1051, 2191, 2596
Hsp92I GRCGYC 2 cut(s) 222, 291
Hsp92II CATG 8 cut(s) 316, 488, 700, 1429, 1959, 2110, 2593, 2726
HspAI GCGC 4 cut(s) 125, 343, 1340, 2737
Kpn2I TCCGGA 1 cut(s) 1327
Ksp22I TGATCA 2 cut(s) 481, 1621
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
LmnI GCTCC 6 cut(s) 108, 394, 773, 1455, 1676, 1842
Lsp1109I GCAGC 2 cut(s) 1118, 1804
LweI GCATC 8 cut(s) 311, 484, 661, 667, 2023, 2138, 2371, 2604
MaeI CTAG 5 cut(s) 338, 557, 996, 1925, 2773
MaeII ACGT 5 cut(s) 291, 844, 1051, 2191, 2596
MaeIII GTNAC 8 cut(s) 889, 1138, 1421, 1529, 1550, 1785, 2183, 2392
MalI GATC 8 cut(s) 371, 483, 1096, 1498, 1623, 1701, 1857, 1878
MbiI CCGCTC 1 cut(s) 103
MboI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
MflI RGATCY 3 cut(s) 1094, 1699, 1855
MhlI GDGCHC 3 cut(s) 770, 835, 1149
MlyI GAGTC 4 cut(s) 78, 388, 887, 2677
MmeI TCCRAC 3 cut(s) 130, 330, 1820
MroI TCCGGA 1 cut(s) 1327
MroXI GAANNNNTTC 2 cut(s) 915, 1908
MslI CAYNNNNRTG 3 cut(s) 489, 2031, 2721
MspA1I CMGCKG 3 cut(s) 353, 2300, 2451
MspI CCGG 4 cut(s) 1328, 2099, 2625, 2764
MspR9I CCNGG 8 cut(s) 837, 1155, 2099, 2100, 2322, 2625, 2626, 2764
Mva1269I GAATGC 2 cut(s) 978, 2758
MvaI CCWGG 3 cut(s) 837, 1155, 2322
MvnI CGCG 3 cut(s) 219, 345, 353
MwoI GCNNNNNNNGC 3 cut(s) 857, 1184, 1826
NciI CCSGG 5 cut(s) 2099, 2100, 2625, 2626, 2764
NdeII GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
NlaIII CATG 8 cut(s) 316, 488, 700, 1429, 1959, 2110, 2593, 2726
NlaIV GGNNCC 4 cut(s) 834, 928, 1838, 2560
NmeAIII GCCGAG 2 cut(s) 250, 258
NmuCI GTSAC 4 cut(s) 1550, 1785, 2183, 2392
NspI RCATGY 1 cut(s) 1429
PagI TCATGA 1 cut(s) 484
PaqCI CACCTGC 1 cut(s) 988
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 2 cut(s) 978, 2758
PdmI GAANNNNTTC 2 cut(s) 915, 1908
PfeI GAWTC 4 cut(s) 854, 1802, 2233, 2294
PfoI TCCNGGA 1 cut(s) 2320
PkrI GCNGC 7 cut(s) 102, 124, 352, 355, 806, 1108, 1819
PleI GAGTC 4 cut(s) 78, 387, 886, 2676
PpsI GAGTC 4 cut(s) 78, 387, 886, 2676
Psp1406I AACGTT 1 cut(s) 2191
Psp6I CCWGG 3 cut(s) 835, 1153, 2320
PspFI CCCAGC 2 cut(s) 356, 721
PspGI CCWGG 3 cut(s) 835, 1153, 2320
PspN4I GGNNCC 4 cut(s) 834, 928, 1838, 2560
PspPI GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2559
PstI CTGCAG 1 cut(s) 1458
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 3 cut(s) 1094, 1699, 1855
PvuII CAGCTG 1 cut(s) 2300
RsaI GTAC 9 cut(s) 287, 323, 514, 527, 1020, 1351, 2125, 2221, 2664
RsaNI GTAC 9 cut(s) 286, 322, 513, 526, 1019, 1350, 2124, 2220, 2663
RseI CAYNNNNRTG 3 cut(s) 489, 2031, 2721
SacII CCGCGG 1 cut(s) 354
SatI GCNGC 7 cut(s) 101, 123, 351, 354, 805, 1107, 1818
Sau3AI GATC 8 cut(s) 369, 481, 1094, 1496, 1621, 1699, 1855, 1876
Sau96I GGNCC 6 cut(s) 47, 927, 999, 1157, 1330, 2559
ScaI AGTACT 3 cut(s) 514, 1351, 2221
SchI GAGTC 4 cut(s) 78, 388, 887, 2677
ScrFI CCNGG 8 cut(s) 837, 1155, 2099, 2100, 2322, 2625, 2626, 2764
SduI GDGCHC 3 cut(s) 770, 835, 1149
SfaNI GCATC 8 cut(s) 311, 484, 661, 667, 2023, 2138, 2371, 2604
SfcI CTRYAG 4 cut(s) 1454, 1884, 2287, 2478
Sfr303I CCGCGG 1 cut(s) 354
SgrBI CCGCGG 1 cut(s) 354
SinI GGWCC 5 cut(s) 47, 927, 1157, 1330, 2559
SmaI CCCGGG 2 cut(s) 2100, 2626
SmiMI CAYNNNNRTG 3 cut(s) 489, 2031, 2721
SspI AATATT 2 cut(s) 1606, 1777
SspMI CTAG 5 cut(s) 338, 557, 996, 1925, 2773
StyD4I CCNGG 8 cut(s) 835, 1153, 2097, 2098, 2320, 2623, 2624, 2762
StyI CCWWGG 3 cut(s) 390, 2052, 2540
TaaI ACNGT 9 cut(s) 56, 178, 1285, 1419, 1440, 1556, 1896, 2219, 2275
TaiI ACGT 5 cut(s) 294, 847, 1054, 2194, 2599
TaqI TCGA 5 cut(s) 8, 441, 823, 2083, 2750
TatI WGTACW 7 cut(s) 512, 525, 1018, 1349, 2123, 2219, 2662
TauI GCSGC 5 cut(s) 103, 125, 353, 356, 807
TfiI GAWTC 4 cut(s) 854, 1802, 2233, 2294
TscAI CASTG 5 cut(s) 1422, 1555, 1738, 1901, 2278
TseFI GTSAC 4 cut(s) 1550, 1785, 2183, 2392
TseI GCWGC 2 cut(s) 1106, 1817
Tsp45I GTSAC 4 cut(s) 1550, 1785, 2183, 2392
TspGWI ACGGA 3 cut(s) 124, 919, 2639
TspMI CCCGGG 2 cut(s) 2098, 2624
TspRI CASTG 5 cut(s) 1422, 1555, 1738, 1901, 2278
VpaK11BI GGWCC 5 cut(s) 47, 927, 1157, 1330, 2559
XapI RAATTY 6 cut(s) 149, 415, 657, 902, 1823, 2152
XceI RCATGY 1 cut(s) 1429
XcmI CCANNNNNNNNNTGG 1 cut(s) 2538
XmaI CCCGGG 2 cut(s) 2098, 2624
XmnI GAANNNNTTC 2 cut(s) 915, 1908
XspI CTAG 5 cut(s) 338, 557, 996, 1925, 2773
ZraI GACGTC 1 cut(s) 292
ZrmI AGTACT 3 cut(s) 514, 1351, 2221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.