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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g23920 FvH4_2g23920 |
| malus_domestica | MD11G1308600.v1.1 |
| prunus_persica | Prupe.8G262800_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr6g0291551 |
| rosa_laevigata | RLG00000012108 |
| rosa_multiflora | Rmu_sc0002226.1_g000002 |
| rosa_roxburghii | Rroxscaffold_7G00175980 |
| rosa_rugosa | Rorug06G0220200 |
| rosa_samantha | Rh6AG332400 Rh6BG339300 Rh6CG345600 Rh6DG332900 |
| rosa_wichuraiana | Rw6G028870 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1045 |
| AatII | GACGTC | 1 cut(s) | 351 |
| Acc36I | ACCTGC | 2 cut(s) | 1045, 1418 |
| AccB1I | GGYRCC | 1 cut(s) | 889 |
| AccII | CGCG | 3 cut(s) | 402, 410, 2882 |
| AccIII | TCCGGA | 1 cut(s) | 1384 |
| AclWI | GGATC | 3 cut(s) | 434, 1561, 2354 |
| AcoI | YGGCCR | 1 cut(s) | 405 |
| AcsI | RAATTY | 8 cut(s) | 206, 472, 714, 959, 1451, 1880, 2209, 2869 |
| AcuI | CTGAAG | 7 cut(s) | 57, 317, 1179, 1454, 1610, 1794, 2265 |
| AcyI | GRCGYC | 2 cut(s) | 279, 348 |
| AdeI | CACNNNGTG | 2 cut(s) | 372, 1696 |
| AfaI | GTAC | 8 cut(s) | 380, 571, 584, 1077, 1408, 2182, 2278, 2697 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 178, 414, 834, 1064, 1527, 2689, 2839 |
| AflIII | ACRYGT | 2 cut(s) | 1265, 2629 |
| AgeI | ACCGGT | 1 cut(s) | 2237 |
| AgsI | TTSAA | 9 cut(s) | 125, 261, 532, 815, 1082, 1251, 1572, 2218, 2741 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 352 |
| AjnI | CCWGG | 4 cut(s) | 137, 892, 1210, 2362 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2069, 2101 |
| Alw21I | GWGCWC | 2 cut(s) | 827, 1206 |
| Alw26I | GTCTC | 7 cut(s) | 119, 270, 1024, 1812, 2165, 2333, 2594 |
| AlwI | GGATC | 3 cut(s) | 434, 1561, 2354 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1169 |
| Ama87I | CYCGRG | 2 cut(s) | 2155, 2657 |
| Aor13HI | TCCGGA | 1 cut(s) | 1384 |
| AoxI | GGCC | 4 cut(s) | 337, 405, 636, 1055 |
| ApeKI | GCWGC | 4 cut(s) | 1040, 1163, 1874, 2823 |
| ApoI | RAATTY | 8 cut(s) | 206, 472, 714, 959, 1451, 1880, 2209, 2869 |
| AsiGI | ACCGGT | 1 cut(s) | 2237 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 972, 1965 |
| AspLEI | GCGC | 5 cut(s) | 184, 402, 1399, 2772, 2882 |
| AspS9I | GGNCC | 5 cut(s) | 104, 984, 1056, 1214, 1387 |
| AsuC2I | CCSGG | 4 cut(s) | 2156, 2157, 2658, 2659 |
| AvaI | CYCGRG | 2 cut(s) | 2155, 2657 |
| AvaII | GGWCC | 4 cut(s) | 104, 984, 1214, 1387 |
| AxyI | CCTNAGG | 1 cut(s) | 1818 |
| BaeGI | GKGCMC | 1 cut(s) | 892 |
| BanI | GGYRCC | 1 cut(s) | 889 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2173, 2205 |
| BbsI | GAAGAC | 2 cut(s) | 1616, 1816 |
| Bbv12I | GWGCWC | 2 cut(s) | 827, 1206 |
| BbvCI | CCTCAGC | 1 cut(s) | 633 |
| BbvI | GCAGC | 4 cut(s) | 1027, 1175, 1861, 2835 |
| BccI | CCATC | 9 cut(s) | 52, 239, 1093, 1184, 1220, 1323, 2018, 2612, 2683 |
| BceAI | ACGGC | 1 cut(s) | 1116 |
| BciT130I | CCWGG | 4 cut(s) | 139, 894, 1212, 2364 |
| BclI | TGATCA | 2 cut(s) | 538, 1678 |
| BcnI | CCSGG | 4 cut(s) | 2156, 2157, 2658, 2659 |
| BcoDI | GTCTC | 7 cut(s) | 119, 270, 1024, 1812, 2165, 2333, 2594 |
| BfaI | CTAG | 9 cut(s) | 395, 608, 614, 725, 1053, 1982, 2490, 2520, 2804 |
| BfmI | CTRYAG | 7 cut(s) | 1041, 1511, 1941, 2193, 2344, 2705, 2891 |
| BfoI | RGCGCY | 2 cut(s) | 185, 1400 |
| BfuAI | ACCTGC | 2 cut(s) | 1045, 1418 |
| BglII | AGATCT | 3 cut(s) | 1151, 1756, 1912 |
| BisI | GCNGC | 7 cut(s) | 180, 408, 411, 1041, 1164, 1875, 2824 |
| BlpI | GCTNAGC | 1 cut(s) | 2466 |
| BlsI | GCNGC | 7 cut(s) | 181, 409, 412, 1042, 1165, 1876, 2825 |
| BmcAI | AGTACT | 2 cut(s) | 571, 2278 |
| Bme1390I | CCNGG | 8 cut(s) | 139, 894, 1212, 2156, 2157, 2364, 2658, 2659 |
| Bme18I | GGWCC | 4 cut(s) | 104, 984, 1214, 1387 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 352 |
| BmeT110I | CYCGRG | 2 cut(s) | 2155, 2657 |
| BmgT120I | GGNCC | 5 cut(s) | 104, 984, 1056, 1214, 1387 |
| BmiI | GGNNCC | 5 cut(s) | 891, 985, 1895, 2791, 2810 |
| BmrFI | CCNGG | 8 cut(s) | 139, 894, 1212, 2156, 2157, 2364, 2658, 2659 |
| BpiI | GAAGAC | 2 cut(s) | 1616, 1816 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2292, 2324 |
| BpmI | CTGGAG | 1 cut(s) | 121 |
| Bpu10I | CCTNAGC | 1 cut(s) | 633 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2466 |
| BpuMI | CCSGG | 4 cut(s) | 2156, 2157, 2658, 2659 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1911 |
| BsaHI | GRCGYC | 2 cut(s) | 279, 348 |
| BsaI | GGTCTC | 4 cut(s) | 119, 2165, 2333, 2594 |
| BsaWI | WCCGGW | 2 cut(s) | 1384, 2237 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 178, 414, 834, 1064, 1527, 2689, 2839 |
| Bse118I | RCCGGY | 1 cut(s) | 2237 |
| Bse1I | ACTGG | 2 cut(s) | 265, 352 |
| Bse21I | CCTNAGG | 1 cut(s) | 1818 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1911 |
| BseAI | TCCGGA | 1 cut(s) | 1384 |
| BseBI | CCWGG | 4 cut(s) | 139, 894, 1212, 2364 |
| BseGI | GGATG | 9 cut(s) | 77, 359, 843, 1108, 1314, 1648, 2095, 2698, 2780 |
| BseJI | GATNNNNATC | 1 cut(s) | 1911 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 178, 414, 834, 1064, 1527, 2689, 2839 |
| BseMII | CTCAG | 7 cut(s) | 624, 1191, 1453, 1501, 1618, 1832, 2457 |
| BseNI | ACTGG | 2 cut(s) | 265, 352 |
| BseRI | GAGGAG | 6 cut(s) | 124, 505, 1307, 1592, 1760, 2538 |
| BseSI | GKGCMC | 1 cut(s) | 892 |
| BseXI | GCAGC | 4 cut(s) | 1027, 1175, 1861, 2835 |
| BseYI | CCCAGC | 3 cut(s) | 413, 778, 2794 |
| Bsh1236I | CGCG | 3 cut(s) | 402, 410, 2882 |
| BshFI | GGCC | 4 cut(s) | 339, 407, 638, 1057 |
| BshNI | GGYRCC | 1 cut(s) | 889 |
| BshTI | ACCGGT | 1 cut(s) | 2237 |
| BsiHKAI | GWGCWC | 2 cut(s) | 827, 1206 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2155, 2657 |
| BsiSI | CCGG | 4 cut(s) | 1385, 2156, 2238, 2658 |
| BslFI | GGGAC | 3 cut(s) | 179, 1630, 2609 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 178, 414, 834, 1064, 1527, 2689, 2839 |
| BsmAI | GTCTC | 7 cut(s) | 119, 270, 1024, 1812, 2165, 2333, 2594 |
| BsmBI | CGTCTC | 1 cut(s) | 270 |
| BsmFI | GGGAC | 3 cut(s) | 179, 1630, 2609 |
| BsmI | GAATGC | 1 cut(s) | 1035 |
| BsnI | GGCC | 4 cut(s) | 339, 407, 638, 1057 |
| Bso31I | GGTCTC | 4 cut(s) | 119, 2165, 2333, 2594 |
| BsoBI | CYCGRG | 2 cut(s) | 2155, 2657 |
| Bsp1286I | GDGCHC | 3 cut(s) | 827, 892, 1206 |
| Bsp13I | TCCGGA | 1 cut(s) | 1384 |
| Bsp143I | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2466 |
| BspANI | GGCC | 4 cut(s) | 339, 407, 638, 1057 |
| BspCNI | CTCAG | 7 cut(s) | 625, 1192, 1452, 1500, 1617, 1831, 2458 |
| BspEI | TCCGGA | 1 cut(s) | 1384 |
| BspFNI | CGCG | 3 cut(s) | 402, 410, 2882 |
| BspHI | TCATGA | 1 cut(s) | 541 |
| BspLI | GGNNCC | 5 cut(s) | 891, 985, 1895, 2791, 2810 |
| BspMAI | CTGCAG | 3 cut(s) | 1045, 1515, 2709 |
| BspMI | ACCTGC | 2 cut(s) | 1045, 1418 |
| BspPI | GGATC | 3 cut(s) | 434, 1561, 2354 |
| BspT107I | GGYRCC | 1 cut(s) | 889 |
| BspTNI | GGTCTC | 4 cut(s) | 119, 2165, 2333, 2594 |
| BsrFI | RCCGGY | 1 cut(s) | 2237 |
| BsrI | ACTGG | 2 cut(s) | 265, 352 |
| BssAI | RCCGGY | 1 cut(s) | 2237 |
| BssMI | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| BssNI | GRCGYC | 2 cut(s) | 279, 348 |
| BssT1I | CCWWGG | 3 cut(s) | 447, 2109, 2582 |
| Bst2UI | CCWGG | 4 cut(s) | 139, 894, 1212, 2364 |
| Bst4CI | ACNGT | 6 cut(s) | 113, 1476, 1497, 1613, 1953, 2276 |
| Bst6I | CTCTTC | 5 cut(s) | 126, 651, 668, 800, 2557 |
| BstACI | GRCGYC | 2 cut(s) | 279, 348 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1049 |
| BstC8I | GCNNGC | 1 cut(s) | 2018 |
| BstDEI | CTNAG | 9 cut(s) | 633, 648, 669, 1200, 1439, 1487, 1604, 1818, 2466 |
| BstDSI | CCRYGG | 2 cut(s) | 408, 1612 |
| BstF5I | GGATG | 9 cut(s) | 77, 359, 843, 1108, 1314, 1648, 2095, 2698, 2780 |
| BstFNI | CGCG | 3 cut(s) | 402, 410, 2882 |
| BstH2I | RGCGCY | 2 cut(s) | 185, 1400 |
| BstHHI | GCGC | 5 cut(s) | 184, 402, 1399, 2772, 2882 |
| BstKTI | GATC | 9 cut(s) | 429, 541, 1154, 1556, 1681, 1759, 1915, 1936, 2362 |
| BstMAI | GTCTC | 7 cut(s) | 119, 270, 1024, 1812, 2165, 2333, 2594 |
| BstMBI | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1049, 1241, 1883 |
| BstNI | CCWGG | 4 cut(s) | 139, 894, 1212, 2364 |
| BstNSI | RCATGY | 2 cut(s) | 1269, 1486 |
| BstSCI | CCNGG | 8 cut(s) | 137, 892, 1210, 2154, 2155, 2362, 2656, 2657 |
| BstSFI | CTRYAG | 7 cut(s) | 1041, 1511, 1941, 2193, 2344, 2705, 2891 |
| BstSLI | GKGCMC | 1 cut(s) | 892 |
| BstUI | CGCG | 3 cut(s) | 402, 410, 2882 |
| BstV1I | GCAGC | 4 cut(s) | 1027, 1175, 1861, 2835 |
| BstV2I | GAAGAC | 2 cut(s) | 1616, 1816 |
| BstX2I | RGATCY | 3 cut(s) | 1151, 1756, 1912 |
| BstYI | RGATCY | 3 cut(s) | 1151, 1756, 1912 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1818 |
| BsuRI | GGCC | 4 cut(s) | 339, 407, 638, 1057 |
| BtgI | CCRYGG | 2 cut(s) | 408, 1612 |
| BtsCI | GGATG | 9 cut(s) | 77, 359, 843, 1108, 1314, 1648, 2095, 2698, 2780 |
| BtsI | GCAGTG | 1 cut(s) | 1795 |
| BtsIMutI | CAGTG | 4 cut(s) | 1472, 1612, 1795, 1958 |
| BveI | ACCTGC | 2 cut(s) | 1045, 1418 |
| Cac8I | GCNNGC | 1 cut(s) | 2018 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1169 |
| CciI | TCATGA | 1 cut(s) | 541 |
| CfoI | GCGC | 5 cut(s) | 184, 402, 1399, 2772, 2882 |
| Cfr10I | RCCGGY | 1 cut(s) | 2237 |
| Cfr13I | GGNCC | 5 cut(s) | 104, 984, 1056, 1214, 1387 |
| Cfr42I | CCGCGG | 1 cut(s) | 411 |
| Cfr9I | CCCGGG | 2 cut(s) | 2155, 2657 |
| CseI | GACGC | 2 cut(s) | 287, 2459 |
| Csp6I | GTAC | 8 cut(s) | 379, 570, 583, 1076, 1407, 2181, 2277, 2696 |
| CspAI | ACCGGT | 1 cut(s) | 2237 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 544, 579, 1711, 1746, 2233, 2268 |
| CviQI | GTAC | 8 cut(s) | 379, 570, 583, 1076, 1407, 2181, 2277, 2696 |
| DdeI | CTNAG | 9 cut(s) | 633, 648, 669, 1200, 1439, 1487, 1604, 1818, 2466 |
| DpnI | GATC | 9 cut(s) | 428, 540, 1153, 1555, 1680, 1758, 1914, 1935, 2361 |
| DpnII | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| DraI | TTTAAA | 1 cut(s) | 72 |
| DraIII | CACNNNGTG | 2 cut(s) | 372, 1696 |
| DriI | GACNNNNNGTC | 1 cut(s) | 352 |
| EaeI | YGGCCR | 1 cut(s) | 405 |
| Eam1104I | CTCTTC | 5 cut(s) | 126, 651, 668, 800, 2557 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 352 |
| EarI | CTCTTC | 5 cut(s) | 126, 651, 668, 800, 2557 |
| EciI | GGCGGA | 1 cut(s) | 2744 |
| Eco130I | CCWWGG | 3 cut(s) | 447, 2109, 2582 |
| Eco31I | GGTCTC | 4 cut(s) | 119, 2165, 2333, 2594 |
| Eco47I | GGWCC | 4 cut(s) | 104, 984, 1214, 1387 |
| Eco57I | CTGAAG | 7 cut(s) | 57, 317, 1179, 1454, 1610, 1794, 2265 |
| Eco81I | CCTNAGG | 1 cut(s) | 1818 |
| Eco88I | CYCGRG | 2 cut(s) | 2155, 2657 |
| EcoRII | CCWGG | 4 cut(s) | 137, 892, 1210, 2362 |
| EcoT14I | CCWWGG | 3 cut(s) | 447, 2109, 2582 |
| ErhI | CCWWGG | 3 cut(s) | 447, 2109, 2582 |
| Esp3I | CGTCTC | 1 cut(s) | 270 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 960, 992, 1659, 1691, 2603, 2635 |
| FaqI | GGGAC | 3 cut(s) | 179, 1630, 2609 |
| FauI | CCCGC | 2 cut(s) | 205, 730 |
| FbaI | TGATCA | 2 cut(s) | 538, 1678 |
| Fnu4HI | GCNGC | 7 cut(s) | 180, 408, 411, 1041, 1164, 1875, 2824 |
| FokI | GGATG | 9 cut(s) | 64, 346, 850, 1115, 1301, 1655, 2102, 2705, 2767 |
| Fsp4HI | GCNGC | 7 cut(s) | 180, 408, 411, 1041, 1164, 1875, 2824 |
| FspBI | CTAG | 9 cut(s) | 395, 608, 614, 725, 1053, 1982, 2490, 2520, 2804 |
| GlaI | GCGC | 5 cut(s) | 183, 401, 1398, 2771, 2881 |
| GluI | GCNGC | 7 cut(s) | 180, 408, 411, 1041, 1164, 1875, 2824 |
| GsaI | CCCAGC | 3 cut(s) | 417, 782, 2798 |
| GsuI | CTGGAG | 1 cut(s) | 121 |
| HaeII | RGCGCY | 2 cut(s) | 185, 1400 |
| HaeIII | GGCC | 4 cut(s) | 339, 407, 638, 1057 |
| HapII | CCGG | 4 cut(s) | 1385, 2156, 2238, 2658 |
| HgaI | GACGC | 2 cut(s) | 287, 2459 |
| HhaI | GCGC | 5 cut(s) | 184, 402, 1399, 2772, 2882 |
| Hin1I | GRCGYC | 2 cut(s) | 279, 348 |
| Hin6I | GCGC | 5 cut(s) | 182, 400, 1397, 2770, 2880 |
| HinP1I | GCGC | 5 cut(s) | 182, 400, 1397, 2770, 2880 |
| HincII | GTYRAC | 2 cut(s) | 440, 1704 |
| HindII | GTYRAC | 2 cut(s) | 440, 1704 |
| HinfI | GANTC | 8 cut(s) | 62, 436, 911, 935, 1859, 2290, 2351, 2701 |
| HpaII | CCGG | 4 cut(s) | 1385, 2156, 2238, 2658 |
| Hpy166II | GTNNAC | 7 cut(s) | 440, 583, 1076, 1493, 1704, 1799, 1961 |
| Hpy188III | TCNNGA | 6 cut(s) | 542, 1026, 1385, 1628, 2141, 2761 |
| Hpy8I | GTNNAC | 7 cut(s) | 440, 583, 1076, 1493, 1704, 1799, 1961 |
| Hpy99I | CGWCG | 3 cut(s) | 308, 350, 2142 |
| HpyAV | CCTTC | 7 cut(s) | 95, 267, 1056, 1269, 1400, 1917, 2495 |
| HpyCH4III | ACNGT | 6 cut(s) | 113, 1476, 1497, 1613, 1953, 2276 |
| HpyCH4IV | ACGT | 4 cut(s) | 348, 901, 1108, 2629 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1049, 1241, 1883 |
| HpyF3I | CTNAG | 9 cut(s) | 633, 648, 669, 1200, 1439, 1487, 1604, 1818, 2466 |
| HpySE526I | ACGT | 4 cut(s) | 348, 901, 1108, 2629 |
| Hsp92I | GRCGYC | 2 cut(s) | 279, 348 |
| HspAI | GCGC | 5 cut(s) | 182, 400, 1397, 2770, 2880 |
| Kpn2I | TCCGGA | 1 cut(s) | 1384 |
| Ksp22I | TGATCA | 2 cut(s) | 538, 1678 |
| KspI | CCGCGG | 1 cut(s) | 411 |
| Kzo9I | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| LmnI | GCTCC | 6 cut(s) | 451, 830, 1512, 1733, 1899, 2795 |
| Lsp1109I | GCAGC | 4 cut(s) | 1027, 1175, 1861, 2835 |
| MaeI | CTAG | 9 cut(s) | 395, 608, 614, 725, 1053, 1982, 2490, 2520, 2804 |
| MaeII | ACGT | 4 cut(s) | 348, 901, 1108, 2629 |
| MaeIII | GTNAC | 7 cut(s) | 946, 1195, 1478, 1586, 1607, 1842, 2240 |
| MalI | GATC | 9 cut(s) | 428, 540, 1153, 1555, 1680, 1758, 1914, 1935, 2361 |
| MboI | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| MflI | RGATCY | 3 cut(s) | 1151, 1756, 1912 |
| MhlI | GDGCHC | 3 cut(s) | 827, 892, 1206 |
| MlyI | GAGTC | 3 cut(s) | 56, 445, 944 |
| MmeI | TCCRAC | 3 cut(s) | 187, 387, 1762 |
| MroI | TCCGGA | 1 cut(s) | 1384 |
| MroXI | GAANNNNTTC | 2 cut(s) | 972, 1965 |
| MslI | CAYNNNNRTG | 5 cut(s) | 56, 546, 2088, 2253, 2754 |
| MspA1I | CMGCKG | 3 cut(s) | 410, 1856, 2357 |
| MspI | CCGG | 4 cut(s) | 1385, 2156, 2238, 2658 |
| MspR9I | CCNGG | 8 cut(s) | 139, 894, 1212, 2156, 2157, 2364, 2658, 2659 |
| Mva1269I | GAATGC | 1 cut(s) | 1035 |
| MvaI | CCWGG | 4 cut(s) | 139, 894, 1212, 2364 |
| MvnI | CGCG | 3 cut(s) | 402, 410, 2882 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1049, 1241, 1883 |
| NciI | CCSGG | 4 cut(s) | 2156, 2157, 2658, 2659 |
| NdeII | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| NlaIV | GGNNCC | 5 cut(s) | 891, 985, 1895, 2791, 2810 |
| NmeAIII | GCCGAG | 2 cut(s) | 307, 315 |
| NmuCI | GTSAC | 3 cut(s) | 1607, 1842, 2240 |
| NspI | RCATGY | 2 cut(s) | 1269, 1486 |
| PagI | TCATGA | 1 cut(s) | 541 |
| PaqCI | CACCTGC | 1 cut(s) | 1045 |
| PciI | ACATGT | 1 cut(s) | 1265 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 342 |
| PctI | GAATGC | 1 cut(s) | 1035 |
| PdmI | GAANNNNTTC | 2 cut(s) | 972, 1965 |
| PfeI | GAWTC | 5 cut(s) | 911, 1859, 2290, 2351, 2701 |
| PfoI | TCCNGGA | 1 cut(s) | 2362 |
| PinAI | ACCGGT | 1 cut(s) | 2237 |
| PkrI | GCNGC | 7 cut(s) | 181, 409, 412, 1042, 1165, 1876, 2825 |
| PleI | GAGTC | 3 cut(s) | 56, 444, 943 |
| PpsI | GAGTC | 3 cut(s) | 56, 444, 943 |
| PscI | ACATGT | 1 cut(s) | 1265 |
| Psp6I | CCWGG | 4 cut(s) | 137, 892, 1210, 2362 |
| PspFI | CCCAGC | 3 cut(s) | 413, 778, 2794 |
| PspGI | CCWGG | 4 cut(s) | 137, 892, 1210, 2362 |
| PspN4I | GGNNCC | 5 cut(s) | 891, 985, 1895, 2791, 2810 |
| PspPI | GGNCC | 5 cut(s) | 104, 984, 1056, 1214, 1387 |
| PstI | CTGCAG | 3 cut(s) | 1045, 1515, 2709 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1169 |
| PsuI | RGATCY | 3 cut(s) | 1151, 1756, 1912 |
| PvuII | CAGCTG | 2 cut(s) | 1856, 2357 |
| RsaI | GTAC | 8 cut(s) | 380, 571, 584, 1077, 1408, 2182, 2278, 2697 |
| RsaNI | GTAC | 8 cut(s) | 379, 570, 583, 1076, 1407, 2181, 2277, 2696 |
| RseI | CAYNNNNRTG | 5 cut(s) | 56, 546, 2088, 2253, 2754 |
| SacII | CCGCGG | 1 cut(s) | 411 |
| SatI | GCNGC | 7 cut(s) | 180, 408, 411, 1041, 1164, 1875, 2824 |
| Sau3AI | GATC | 9 cut(s) | 426, 538, 1151, 1553, 1678, 1756, 1912, 1933, 2359 |
| Sau96I | GGNCC | 5 cut(s) | 104, 984, 1056, 1214, 1387 |
| ScaI | AGTACT | 2 cut(s) | 571, 2278 |
| SchI | GAGTC | 3 cut(s) | 56, 445, 944 |
| ScrFI | CCNGG | 8 cut(s) | 139, 894, 1212, 2156, 2157, 2364, 2658, 2659 |
| SduI | GDGCHC | 3 cut(s) | 827, 892, 1206 |
| SfcI | CTRYAG | 7 cut(s) | 1041, 1511, 1941, 2193, 2344, 2705, 2891 |
| Sfr303I | CCGCGG | 1 cut(s) | 411 |
| SgrBI | CCGCGG | 1 cut(s) | 411 |
| SinI | GGWCC | 4 cut(s) | 104, 984, 1214, 1387 |
| SmaI | CCCGGG | 2 cut(s) | 2157, 2659 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 56, 546, 2088, 2253, 2754 |
| SspI | AATATT | 2 cut(s) | 1663, 1834 |
| SspMI | CTAG | 9 cut(s) | 395, 608, 614, 725, 1053, 1982, 2490, 2520, 2804 |
| StyD4I | CCNGG | 8 cut(s) | 137, 892, 1210, 2154, 2155, 2362, 2656, 2657 |
| StyI | CCWWGG | 3 cut(s) | 447, 2109, 2582 |
| TaaI | ACNGT | 6 cut(s) | 113, 1476, 1497, 1613, 1953, 2276 |
| TaiI | ACGT | 4 cut(s) | 351, 904, 1111, 2632 |
| TaqI | TCGA | 6 cut(s) | 19, 65, 498, 505, 880, 2140 |
| TatI | WGTACW | 5 cut(s) | 569, 582, 1075, 2276, 2695 |
| TauI | GCSGC | 3 cut(s) | 182, 410, 413 |
| TfiI | GAWTC | 5 cut(s) | 911, 1859, 2290, 2351, 2701 |
| TscAI | CASTG | 4 cut(s) | 1479, 1612, 1795, 1958 |
| TseFI | GTSAC | 3 cut(s) | 1607, 1842, 2240 |
| TseI | GCWGC | 4 cut(s) | 1040, 1163, 1874, 2823 |
| Tsp45I | GTSAC | 3 cut(s) | 1607, 1842, 2240 |
| TspGWI | ACGGA | 5 cut(s) | 181, 976, 2463, 2672, 2840 |
| TspMI | CCCGGG | 2 cut(s) | 2155, 2657 |
| TspRI | CASTG | 4 cut(s) | 1479, 1612, 1795, 1958 |
| VpaK11BI | GGWCC | 4 cut(s) | 104, 984, 1214, 1387 |
| XapI | RAATTY | 8 cut(s) | 206, 472, 714, 959, 1451, 1880, 2209, 2869 |
| XceI | RCATGY | 2 cut(s) | 1269, 1486 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2580 |
| XmaI | CCCGGG | 2 cut(s) | 2155, 2657 |
| XmnI | GAANNNNTTC | 2 cut(s) | 972, 1965 |
| XspI | CTAG | 9 cut(s) | 395, 608, 614, 725, 1053, 1982, 2490, 2520, 2804 |
| ZraI | GACGTC | 1 cut(s) | 349 |
| ZrmI | AGTACT | 2 cut(s) | 571, 2278 |