Rw6G028870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016760
Physical Location & Seq
Forward (+)
52933670 .. 52938190
4521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028870.1

Sequence Viewer

Length: 2850 bp
ATGGACTCGATTTTAAACTCATCCTTGCAAGAAATCTGCTCGGAAGGTCCAAAGGGTCTCCCTCTTCGAACCCTCTCTTCCAGGCTCAACCTCTCTCCGCCACTCCAACAACCCCTATGGGCGGCGCTACTCTCCGTCCCCGCCCTCAAATTCCACGCCCAAACCCAAAACGCCACCGTTTCGCACCTCCCCACAGACCCTTCAATCCAGTCGTTCCGCGACGCCGAGAAGCTCAACCTGAAGCTCGTCGCCGACCAGCCTCTCCGCAACAACTTCCTCGGCCTCTACGGCGTCCAGTCCGCATCCGACACCATGTGCGAGTACCAACGCAAAACCCTAGAGCGCGTGGCCGCGGCTGGGTCAAAGGGGATCACGCAGAGTCAACTATCCAAGGAGCTTGGGATTAGAGGTGAGAATTTCTTTTACAAATTGAGGAGGCTCGAATGTCAGGGCTTGATTGTGAGGCAACCATTGAATGATGATCATCATGATGATGATGCCAATGTGAGTGGGAGTACTAATTTAGTGTACTTAAATCGCTATGCAAAGCATTTAGGTCTAGGGTCTGAACAAAAAGCTGAGGTCACTAAATCTAAGGAGGAAGAGAATATGACTAAGAAGAGTGGTAATTTGATAGCGGAACTTCCGATTGAGCATCAAATTTACGAGCTAGTTAATGATGCGGGGTCAAGGGGGGTCGTGGTGTTTAAGGTTGCAGAGAGGCTGGGAATTGATAGGAAAGAGAATAGGAAGAGGATTGAAAGTTTGTGCTCCAAGTTTGGGATGATAATGGATAAAGAGAAATGCGGAAAGACCAAAGTGTATCGAGTTTGGGCACCTGGGAAACGTAATGCTGAATCAGCTAATGCAATTCTGAATGAGTCTGAAAATGTTACTCAAAGTAAATTTAATGAAGATGTTCTTCGTGGGTCCGTTCAGAAAGGTGATACAGCAATAAATGATGTCTCATCAGGAATGCCACCTGCTGTGGAATGTGCTAGGCCCAGAAGGGGAAAAAGTGTACTTGAACCATCACAGGATAAAGGGGATGAACGTCAATCTCTGGGAATTAGAAGCCCTTTGCAGAAAAGAAAGAGATCTTCTGAAGCAGCTTCTGTAAAGTTTATAGAAGTTGATGGTGTAACTGAGCACCAACCTGGACCAAATGAAGATGGTGAGGCGAGGTGTGCTTTTTCAAAGTCAAAATCAACATGTCAAAGAAGGTTTTTATGGACAGAGGAGGCTGATAGGCAATTAGTTATCCAATATGTAAGACGGTGTGCAACTCTTGGGGCAAAATATCATCACCAGATAAATTGGGCTTCACTTTCGGACCTTCCAGCTCCTCCAAGTACCTGCAAAAGAAGAATGTCATATCTGAAGCTCAGTAATGGAAAATTTAGAAAATCTTTAATGACACTGTGTAACATGCTCAGTAAACGGTATGCAAAGCTCCTGCAGAAAACCCAGAACAGGCCATTTTACCAAGATGATTGTGGATCACTTTTGCAAGGTTCAACAGAGGAGGGTTGTAAACAGAATTATCCTCTCAGTGACCATGGGACAGGTCTTCAGGAAGAACAATGGGATGACTTTAATGACAAAAATATTAAAGAAGTGCTTGATCAGATACTTCACTATGTGAGGTTGACTAAATCTGTTGCCTCCAAAAGATGTGGAGCTTCCTGCGAGGAGGGTTCAGAACTTAATGCTAATGCTGAAGATGTTGGAGGCTGTGATTGTGAACCTTTGTCTCTCTCACCTCAGGTCTTCCCCAATATTTGCAAGTCACCATTTCCAACTGATTCCGGCATGAGAGCTGCCAAATTTGCTCATTGGCTCCACAAAAGAGATAGAGATCTTATGGGAAGGGGGCTTGATCTTCCTACAGATATACAGTGTGGTGAATTTTTTCATATGTTTGCTCTAGTTTTTTCAGGTGAATTGTCAATCTCCCCATGCTTGCCAGATGGAGTGGGAGAAGCAGAAGATTTGAGAAGCTCAAAACATAAAATTGGTAGGAATAAACATTCGGATGCTAAAAGGCTAAAATCCTTGGTTGCAGAAAGTGAGATTATTTCCCATCGAGAAAAAGGTTTCCCGGGTCTCATGGTATCTATATGTCGTACAGCATCTTCTAAAGCCTATTGTGAAAATTTGTTCAAAGATGATGATGATACTCTAGGTGGTGACAAACGTTTTGGTGAAAATTATCAGCAAGACAGTACTTCAGGTGTGAATCAGATGAGAGAAAACCTCAATTCAGGCATTGTTATCCCTGTGGCAGAGACTTGTAGTGAGTCACCTGGCGGAGGTATCCTCCATCCTGGAAATTGTGATATTATTGGAACTCTTTTGATAGGGAAAACAAATATGAATGTTAATGATGCACATAATGTGACTATTCTTAATTCTCCTAAAGAGGTTGATGAGCGTCCGTCTGAAAAACTAACTAGCGGTGTGGAAGGCAAAGTTATCTCACCTAGAGGAGATTTTGATGAATGCTCTGAACTCTCTTCTGATGAATTATGTGTGCCACTTCTTCCTTGGATAAATGGGGACGAGACCATCAACAAGACTATCTACAAGGGATTCATGCAACGTGTTTTTGGGATTGTGATGCAAAACCCTGGGATATTAGAGGGTGAAATTATCCGTAAGATGGATGTACTGAATCCGCAGAGTTGTAGAAAATTGTTGGTGGAGATGATTGAAGCAAAACATCTTCATGTCAGGACAATGCGCCAAGCCACATTGAATGGCCCCCCAGCATTTCTAGGGAACCTATTTGGAAGCAGCCTCACCCAGTCAAGGTGGTTTTCCGTAGGCATCTCTTTGCAAATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

949

Amino Acids

105.4

Weight (kDa)

7.86

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 96 7.9e-25 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 108 - 183 9.7e-14 B-block binding subunit of TFIIIC
DUF7646 PF24657 210 - 286 7.8e-28 Domain of unknown function (DUF7646)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016760)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23920 FvH4_2g23920
malus_domestica MD11G1308600.v1.1
prunus_persica Prupe.8G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0291551
rosa_laevigata RLG00000012108
rosa_multiflora Rmu_sc0002226.1_g000002
rosa_roxburghii Rroxscaffold_7G00175980
rosa_rugosa Rorug06G0220200
rosa_samantha Rh6AG332400 Rh6BG339300 Rh6CG345600 Rh6DG332900
rosa_wichuraiana Rw6G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 991
Acc36I ACCTGC 2 cut(s) 991, 1364
AccB1I GGYRCC 1 cut(s) 835
AccII CGCG 3 cut(s) 219, 345, 353
AclI AACGTT 1 cut(s) 2197
AclWI GGATC 2 cut(s) 377, 1507
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 9 cut(s) 149, 415, 660, 905, 1397, 1826, 1907, 2155, 2841
AcuI CTGAAG 6 cut(s) 260, 1125, 1400, 1556, 1740, 2214
AcyI GRCGYC 2 cut(s) 222, 291
AdeI CACNNNGTG 2 cut(s) 315, 1642
AfaI GTAC 8 cut(s) 323, 517, 530, 1023, 1354, 2128, 2227, 2670
AfiI CCNNNNNNNGG 8 cut(s) 121, 357, 780, 1010, 1473, 2312, 2662, 2811
AflIII ACRYGT 2 cut(s) 1211, 2602
AgsI TTSAA 9 cut(s) 204, 475, 761, 1028, 1197, 1518, 2164, 2714, 2758
AjnI CCWGG 6 cut(s) 80, 838, 1156, 2305, 2326, 2629
Alw21I GWGCWC 2 cut(s) 773, 1152
Alw26I GTCTC 6 cut(s) 62, 970, 1758, 2111, 2282, 2558
AlwI GGATC 2 cut(s) 377, 1507
AlwNI CAGNNNCTG 1 cut(s) 1115
Ama87I CYCGRG 1 cut(s) 2101
AoxI GGCC 5 cut(s) 280, 348, 1001, 1475, 2761
ApeKI GCWGC 3 cut(s) 1109, 1820, 2796
ApoI RAATTY 9 cut(s) 149, 415, 660, 905, 1397, 1826, 1907, 2155, 2841
ArsI GACNNNNNNTTYG 2 cut(s) 2183, 2215
Asp700I GAANNNNTTC 2 cut(s) 918, 1911
AspLEI GCGC 3 cut(s) 127, 345, 2745
AspS9I GGNCC 6 cut(s) 47, 930, 1002, 1160, 1333, 2762
AsuC2I CCSGG 2 cut(s) 2102, 2103
AsuII TTCGAA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 2101
AvaII GGWCC 4 cut(s) 47, 930, 1160, 1333
AxyI CCTNAGG 1 cut(s) 1764
BaeGI GKGCMC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 835
BarI GAAGNNNNNNTAC 2 cut(s) 2119, 2151
BbsI GAAGAC 2 cut(s) 1562, 1762
Bbv12I GWGCWC 2 cut(s) 773, 1152
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 3 cut(s) 1121, 1807, 2808
BccI CCATC 8 cut(s) 1039, 1130, 1166, 1964, 2091, 2331, 2576, 2656
BceAI ACGGC 1 cut(s) 304
BciT130I CCWGG 6 cut(s) 82, 840, 1158, 2307, 2328, 2631
BciVI GTATCC 1 cut(s) 2327
BclI TGATCA 2 cut(s) 481, 1624
BcnI CCSGG 2 cut(s) 2102, 2103
BcoDI GTCTC 6 cut(s) 62, 970, 1758, 2111, 2282, 2558
BfaI CTAG 9 cut(s) 338, 560, 671, 999, 1928, 2183, 2454, 2484, 2777
BfmI CTRYAG 2 cut(s) 1457, 1887
BfoI RGCGCY 1 cut(s) 128
BfuAI ACCTGC 2 cut(s) 991, 1364
BfuI GTATCC 1 cut(s) 2327
BglI GCCNNNNNGGC 1 cut(s) 288
BglII AGATCT 2 cut(s) 1097, 1858
BisI GCNGC 6 cut(s) 123, 351, 354, 1110, 1821, 2797
BlsI GCNGC 6 cut(s) 124, 352, 355, 1111, 1822, 2798
BmcAI AGTACT 2 cut(s) 517, 2227
Bme1390I CCNGG 8 cut(s) 82, 840, 1158, 2102, 2103, 2307, 2328, 2631
Bme18I GGWCC 4 cut(s) 47, 930, 1160, 1333
BmeT110I CYCGRG 1 cut(s) 2101
BmgT120I GGNCC 6 cut(s) 47, 930, 1002, 1160, 1333, 2762
BmiI GGNNCC 5 cut(s) 837, 931, 1841, 2764, 2783
BmrFI CCNGG 8 cut(s) 82, 840, 1158, 2102, 2103, 2307, 2328, 2631
BmrI ACTGGG 1 cut(s) 2800
BmsI GCATC 9 cut(s) 311, 487, 664, 670, 2026, 2141, 2377, 2610, 2838
BmuI ACTGGG 1 cut(s) 2800
BpiI GAAGAC 2 cut(s) 1562, 1762
BplI GAGNNNNNCTC 4 cut(s) 2241, 2273, 2304, 2336
Bpu10I CCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 67
BpuMI CCSGG 2 cut(s) 2102, 2103
BsaBI GATNNNNATC 2 cut(s) 483, 1857
BsaHI GRCGYC 2 cut(s) 222, 291
BsaI GGTCTC 3 cut(s) 62, 2111, 2558
Bsc4I CCNNNNNNNGG 8 cut(s) 121, 357, 780, 1010, 1473, 2312, 2662, 2811
Bse1I ACTGG 3 cut(s) 208, 295, 2806
Bse21I CCTNAGG 1 cut(s) 1764
Bse8I GATNNNNATC 2 cut(s) 483, 1857
BseBI CCWGG 6 cut(s) 82, 840, 1158, 2307, 2328, 2631
BseGI GGATG 8 cut(s) 20, 302, 789, 1054, 1594, 2041, 2323, 2671
BseJI GATNNNNATC 2 cut(s) 483, 1857
BseLI CCNNNNNNNGG 8 cut(s) 121, 357, 780, 1010, 1473, 2312, 2662, 2811
BseMII CTCAG 6 cut(s) 570, 1137, 1399, 1447, 1564, 1778
BseNI ACTGG 3 cut(s) 208, 295, 2806
BseRI GAGGAG 6 cut(s) 448, 1253, 1335, 1538, 1706, 2502
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 3 cut(s) 1121, 1807, 2808
BseYI CCCAGC 3 cut(s) 356, 724, 2767
Bsh1236I CGCG 3 cut(s) 219, 345, 353
BshFI GGCC 5 cut(s) 282, 350, 1003, 1477, 2763
BshNI GGYRCC 1 cut(s) 835
BsiHKAI GWGCWC 2 cut(s) 773, 1152
BsiHKCI CYCGRG 1 cut(s) 2101
BsiSI CCGG 2 cut(s) 1809, 2102
BslFI GGGAC 3 cut(s) 122, 1576, 2573
BslI CCNNNNNNNGG 8 cut(s) 121, 357, 780, 1010, 1473, 2312, 2662, 2811
BsmAI GTCTC 6 cut(s) 62, 970, 1758, 2111, 2282, 2558
BsmFI GGGAC 3 cut(s) 122, 1576, 2573
BsmI GAATGC 2 cut(s) 981, 2507
BsnI GGCC 5 cut(s) 282, 350, 1003, 1477, 2763
Bso31I GGTCTC 3 cut(s) 62, 2111, 2558
BsoBI CYCGRG 1 cut(s) 2101
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 3 cut(s) 773, 838, 1152
Bsp143I GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
Bsp19I CCATGG 1 cut(s) 1558
BspANI GGCC 5 cut(s) 282, 350, 1003, 1477, 2763
BspCNI CTCAG 6 cut(s) 571, 1138, 1398, 1446, 1563, 1777
BspFNI CGCG 3 cut(s) 219, 345, 353
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 5 cut(s) 837, 931, 1841, 2764, 2783
BspMAI CTGCAG 1 cut(s) 1461
BspMI ACCTGC 2 cut(s) 991, 1364
BspPI GGATC 2 cut(s) 377, 1507
BspT104I TTCGAA 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 835
BspTNI GGTCTC 3 cut(s) 62, 2111, 2558
BsrI ACTGG 3 cut(s) 208, 295, 2806
BssMI GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
BssNI GRCGYC 2 cut(s) 222, 291
BssT1I CCWWGG 4 cut(s) 390, 1558, 2055, 2546
Bst2UI CCWGG 6 cut(s) 82, 840, 1158, 2307, 2328, 2631
Bst4CI ACNGT 6 cut(s) 178, 1278, 1422, 1443, 1899, 2225
Bst6I CTCTTC 6 cut(s) 69, 82, 597, 614, 746, 2521
BstACI GRCGYC 2 cut(s) 222, 291
BstBI TTCGAA 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 1964
BstDEI CTNAG 8 cut(s) 579, 594, 615, 1146, 1385, 1433, 1550, 1764
BstDSI CCRYGG 2 cut(s) 351, 1558
BstENI CCTNNNNNAGG 1 cut(s) 2310
BstF5I GGATG 8 cut(s) 20, 302, 789, 1054, 1594, 2041, 2323, 2671
BstFNI CGCG 3 cut(s) 219, 345, 353
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 3 cut(s) 127, 345, 2745
BstKTI GATC 7 cut(s) 372, 484, 1100, 1502, 1627, 1861, 1882
BstMAI GTCTC 6 cut(s) 62, 970, 1758, 2111, 2282, 2558
BstMBI GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
BstMWI GCNNNNNNNGC 4 cut(s) 288, 860, 1187, 1829
BstNI CCWGG 6 cut(s) 82, 840, 1158, 2307, 2328, 2631
BstNSI RCATGY 2 cut(s) 1215, 1432
BstSCI CCNGG 8 cut(s) 80, 838, 1156, 2100, 2101, 2305, 2326, 2629
BstSFI CTRYAG 2 cut(s) 1457, 1887
BstSLI GKGCMC 1 cut(s) 838
BstUI CGCG 3 cut(s) 219, 345, 353
BstV1I GCAGC 3 cut(s) 1121, 1807, 2808
BstV2I GAAGAC 2 cut(s) 1562, 1762
BstX2I RGATCY 2 cut(s) 1097, 1858
BstYI RGATCY 2 cut(s) 1097, 1858
Bsu36I CCTNAGG 1 cut(s) 1764
BsuI GTATCC 1 cut(s) 2327
BsuRI GGCC 5 cut(s) 282, 350, 1003, 1477, 2763
BtgI CCRYGG 2 cut(s) 351, 1558
BtsCI GGATG 8 cut(s) 20, 302, 789, 1054, 1594, 2041, 2323, 2671
BtsIMutI CAGTG 3 cut(s) 1418, 1558, 1904
BveI ACCTGC 2 cut(s) 991, 1364
Cac8I GCNNGC 1 cut(s) 1964
CaiI CAGNNNCTG 1 cut(s) 1115
CciI TCATGA 1 cut(s) 487
CfoI GCGC 3 cut(s) 127, 345, 2745
Cfr13I GGNCC 6 cut(s) 47, 930, 1002, 1160, 1333, 2762
Cfr42I CCGCGG 1 cut(s) 354
Cfr9I CCCGGG 1 cut(s) 2101
CseI GACGC 3 cut(s) 230, 280, 2423
Csp6I GTAC 8 cut(s) 322, 516, 529, 1022, 1353, 2127, 2226, 2669
CspCI CAANNNNNGTGG 4 cut(s) 490, 525, 1657, 1692
CviQI GTAC 8 cut(s) 322, 516, 529, 1022, 1353, 2127, 2226, 2669
DdeI CTNAG 8 cut(s) 579, 594, 615, 1146, 1385, 1433, 1550, 1764
DpnI GATC 7 cut(s) 371, 483, 1099, 1501, 1626, 1860, 1881
DpnII GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 2 cut(s) 315, 1642
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 6 cut(s) 69, 82, 597, 614, 746, 2521
EarI CTCTTC 6 cut(s) 69, 82, 597, 614, 746, 2521
EciI GGCGGA 2 cut(s) 87, 2325
Eco130I CCWWGG 4 cut(s) 390, 1558, 2055, 2546
Eco31I GGTCTC 3 cut(s) 62, 2111, 2558
Eco47I GGWCC 4 cut(s) 47, 930, 1160, 1333
Eco57I CTGAAG 6 cut(s) 260, 1125, 1400, 1556, 1740, 2214
Eco81I CCTNAGG 1 cut(s) 1764
Eco88I CYCGRG 1 cut(s) 2101
EcoNI CCTNNNNNAGG 1 cut(s) 2310
EcoRII CCWGG 6 cut(s) 80, 838, 1156, 2305, 2326, 2629
EcoT14I CCWWGG 4 cut(s) 390, 1558, 2055, 2546
ErhI CCWWGG 4 cut(s) 390, 1558, 2055, 2546
FalI AAGNNNNNCTT 4 cut(s) 906, 938, 1605, 1637
FaqI GGGAC 3 cut(s) 122, 1576, 2573
FauI CCCGC 2 cut(s) 148, 676
FauNDI CATATG 1 cut(s) 1917
FbaI TGATCA 2 cut(s) 481, 1624
Fnu4HI GCNGC 6 cut(s) 123, 351, 354, 1110, 1821, 2797
FokI GGATG 8 cut(s) 7, 289, 796, 1061, 1601, 2048, 2310, 2678
Fsp4HI GCNGC 6 cut(s) 123, 351, 354, 1110, 1821, 2797
FspBI CTAG 9 cut(s) 338, 560, 671, 999, 1928, 2183, 2454, 2484, 2777
GlaI GCGC 3 cut(s) 126, 344, 2744
GluI GCNGC 6 cut(s) 123, 351, 354, 1110, 1821, 2797
GsaI CCCAGC 3 cut(s) 360, 728, 2771
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 5 cut(s) 282, 350, 1003, 1477, 2763
HapII CCGG 2 cut(s) 1809, 2102
HgaI GACGC 3 cut(s) 230, 280, 2423
HhaI GCGC 3 cut(s) 127, 345, 2745
Hin1I GRCGYC 2 cut(s) 222, 291
Hin6I GCGC 3 cut(s) 125, 343, 2743
HinP1I GCGC 3 cut(s) 125, 343, 2743
HincII GTYRAC 2 cut(s) 383, 1650
HindII GTYRAC 2 cut(s) 383, 1650
HinfI GANTC 9 cut(s) 5, 379, 857, 881, 1805, 2239, 2300, 2592, 2674
HpaII CCGG 2 cut(s) 1809, 2102
Hpy166II GTNNAC 7 cut(s) 383, 529, 1022, 1439, 1535, 1650, 1745
Hpy188III TCNNGA 5 cut(s) 488, 972, 1574, 2087, 2734
Hpy8I GTNNAC 7 cut(s) 383, 529, 1022, 1439, 1535, 1650, 1745
Hpy99I CGWCG 2 cut(s) 224, 251
HpyAV CCTTC 7 cut(s) 38, 210, 1002, 1215, 1346, 1863, 2459
HpyCH4III ACNGT 6 cut(s) 178, 1278, 1422, 1443, 1899, 2225
HpyCH4IV ACGT 4 cut(s) 847, 1054, 2197, 2602
HpyF10VI GCNNNNNNNGC 4 cut(s) 288, 860, 1187, 1829
HpyF3I CTNAG 8 cut(s) 579, 594, 615, 1146, 1385, 1433, 1550, 1764
HpySE526I ACGT 4 cut(s) 847, 1054, 2197, 2602
Hsp92I GRCGYC 2 cut(s) 222, 291
HspAI GCGC 3 cut(s) 125, 343, 2743
Ksp22I TGATCA 2 cut(s) 481, 1624
KspI CCGCGG 1 cut(s) 354
Kzo9I GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
LmnI GCTCC 6 cut(s) 394, 776, 1348, 1458, 1679, 1845
Lsp1109I GCAGC 3 cut(s) 1121, 1807, 2808
LweI GCATC 9 cut(s) 311, 487, 664, 670, 2026, 2141, 2377, 2610, 2838
MaeI CTAG 9 cut(s) 338, 560, 671, 999, 1928, 2183, 2454, 2484, 2777
MaeII ACGT 4 cut(s) 847, 1054, 2197, 2602
MaeIII GTNAC 9 cut(s) 583, 892, 1141, 1424, 1553, 1788, 2189, 2301, 2398
MalI GATC 7 cut(s) 371, 483, 1099, 1501, 1626, 1860, 1881
MboI GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
MflI RGATCY 2 cut(s) 1097, 1858
MhlI GDGCHC 3 cut(s) 773, 838, 1152
MlyI GAGTC 3 cut(s) 388, 890, 2309
MmeI TCCRAC 4 cut(s) 130, 330, 1708, 1823
MroXI GAANNNNTTC 2 cut(s) 918, 1911
MslI CAYNNNNRTG 4 cut(s) 489, 492, 2034, 2727
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 1809, 2102
MspR9I CCNGG 8 cut(s) 82, 840, 1158, 2102, 2103, 2307, 2328, 2631
Mva1269I GAATGC 2 cut(s) 981, 2507
MvaI CCWGG 6 cut(s) 82, 840, 1158, 2307, 2328, 2631
MvnI CGCG 3 cut(s) 219, 345, 353
MwoI GCNNNNNNNGC 4 cut(s) 288, 860, 1187, 1829
NciI CCSGG 2 cut(s) 2102, 2103
NcoI CCATGG 1 cut(s) 1558
NdeI CATATG 1 cut(s) 1917
NdeII GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
NlaIV GGNNCC 5 cut(s) 837, 931, 1841, 2764, 2783
NmeAIII GCCGAG 2 cut(s) 250, 258
NmuCI GTSAC 6 cut(s) 583, 1553, 1788, 2189, 2301, 2398
NspI RCATGY 2 cut(s) 1215, 1432
NspV TTCGAA 1 cut(s) 67
PagI TCATGA 1 cut(s) 487
PaqCI CACCTGC 1 cut(s) 991
PasI CCCWGGG 1 cut(s) 2630
PciI ACATGT 1 cut(s) 1211
PctI GAATGC 2 cut(s) 981, 2507
PdmI GAANNNNTTC 2 cut(s) 918, 1911
PfeI GAWTC 5 cut(s) 857, 1805, 2239, 2592, 2674
PfoI TCCNGGA 1 cut(s) 2326
PkrI GCNGC 6 cut(s) 124, 352, 355, 1111, 1822, 2798
PleI GAGTC 3 cut(s) 387, 889, 2308
PpsI GAGTC 3 cut(s) 387, 889, 2308
PscI ACATGT 1 cut(s) 1211
Psp1406I AACGTT 1 cut(s) 2197
Psp6I CCWGG 6 cut(s) 80, 838, 1156, 2305, 2326, 2629
PspFI CCCAGC 3 cut(s) 356, 724, 2767
PspGI CCWGG 6 cut(s) 80, 838, 1156, 2305, 2326, 2629
PspN4I GGNNCC 5 cut(s) 837, 931, 1841, 2764, 2783
PspPI GGNCC 6 cut(s) 47, 930, 1002, 1160, 1333, 2762
PstI CTGCAG 1 cut(s) 1461
PstNI CAGNNNCTG 1 cut(s) 1115
PsuI RGATCY 2 cut(s) 1097, 1858
RsaI GTAC 8 cut(s) 323, 517, 530, 1023, 1354, 2128, 2227, 2670
RsaNI GTAC 8 cut(s) 322, 516, 529, 1022, 1353, 2127, 2226, 2669
RseI CAYNNNNRTG 4 cut(s) 489, 492, 2034, 2727
SacII CCGCGG 1 cut(s) 354
SatI GCNGC 6 cut(s) 123, 351, 354, 1110, 1821, 2797
Sau3AI GATC 7 cut(s) 369, 481, 1097, 1499, 1624, 1858, 1879
Sau96I GGNCC 6 cut(s) 47, 930, 1002, 1160, 1333, 2762
ScaI AGTACT 2 cut(s) 517, 2227
SchI GAGTC 3 cut(s) 388, 890, 2309
ScrFI CCNGG 8 cut(s) 82, 840, 1158, 2102, 2103, 2307, 2328, 2631
SduI GDGCHC 3 cut(s) 773, 838, 1152
SfaNI GCATC 9 cut(s) 311, 487, 664, 670, 2026, 2141, 2377, 2610, 2838
SfcI CTRYAG 2 cut(s) 1457, 1887
Sfr303I CCGCGG 1 cut(s) 354
SfuI TTCGAA 1 cut(s) 67
SgrBI CCGCGG 1 cut(s) 354
SinI GGWCC 4 cut(s) 47, 930, 1160, 1333
SmaI CCCGGG 1 cut(s) 2103
SmiMI CAYNNNNRTG 4 cut(s) 489, 492, 2034, 2727
SspI AATATT 2 cut(s) 1609, 1780
SspMI CTAG 9 cut(s) 338, 560, 671, 999, 1928, 2183, 2454, 2484, 2777
StyD4I CCNGG 8 cut(s) 80, 838, 1156, 2100, 2101, 2305, 2326, 2629
StyI CCWWGG 4 cut(s) 390, 1558, 2055, 2546
TaaI ACNGT 6 cut(s) 178, 1278, 1422, 1443, 1899, 2225
TaiI ACGT 4 cut(s) 850, 1057, 2200, 2605
TaqI TCGA 5 cut(s) 8, 67, 441, 826, 2086
TatI WGTACW 5 cut(s) 515, 528, 1021, 2225, 2668
TauI GCSGC 3 cut(s) 125, 353, 356
TfiI GAWTC 5 cut(s) 857, 1805, 2239, 2592, 2674
TscAI CASTG 3 cut(s) 1425, 1558, 1904
TseFI GTSAC 6 cut(s) 583, 1553, 1788, 2189, 2301, 2398
TseI GCWGC 3 cut(s) 1109, 1820, 2796
Tsp45I GTSAC 6 cut(s) 583, 1553, 1788, 2189, 2301, 2398
TspDTI ATGAA 9 cut(s) 927, 1065, 1182, 1904, 2390, 2514, 2538, 2584, 2717
TspGWI ACGGA 5 cut(s) 124, 922, 2427, 2645, 2812
TspMI CCCGGG 1 cut(s) 2101
TspRI CASTG 3 cut(s) 1425, 1558, 1904
VpaK11BI GGWCC 4 cut(s) 47, 930, 1160, 1333
XagI CCTNNNNNAGG 1 cut(s) 2310
XapI RAATTY 9 cut(s) 149, 415, 660, 905, 1397, 1826, 1907, 2155, 2841
XceI RCATGY 2 cut(s) 1215, 1432
XcmI CCANNNNNNNNNTGG 2 cut(s) 1493, 2544
XmaI CCCGGG 1 cut(s) 2101
XmnI GAANNNNTTC 2 cut(s) 918, 1911
XspI CTAG 9 cut(s) 338, 560, 671, 999, 1928, 2183, 2454, 2484, 2777
ZrmI AGTACT 2 cut(s) 517, 2227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.