MD12G1009400.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015242
Physical Location & Seq
Forward (+)
990430 .. 991982
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009400.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCTGATCCGGCAGTACAACGAGACCATGGCACGTCTGAAAGCCGAAGGAAGGGCACATCCATTGGAGGGGGAGGCAAGCGTGCGTCTATGGAGGCGGCAGTGGGAACGACAACAATAAATAGTAATGCAGTAGGTTCTGGTGGTGGTGGTGGTGGTGGTGCAGCACCATGTGGGGCGTGCAAGTTTTTGAGGAGGAAGTGCATAAGCGGGTGCATATTCGCACCCTATTTCGGTTCAGACCAAGGAGCTGCACGCTTTGCAGCTGTGCACAAGGTGTTTGGGGCGAGTAATGTCTCGAAGCTTTTGTTGCACATTCCCTTGAACCGCCGGCATGACGCTGTCGTTACCATTTCCTACGAGGCTCAAGCTAGGCTCTCCGACCCCGTTTATGGTTGTGTCTCCACCATACTTGCTTTGCAACAACAGGTTGCATCTTTGCAAGCCGAGCTAGCAATGGTGCAAACTCAGCTGATAAACAGCAGGTTTGCATTTGCAAACGCTCTCCAAATCACCTCGCAGCAGCAACAGCATGAGCAGCAGCAGAGCCTGGCACTAATGTTGCAGCCTGCTTACTCTAACAACTCCTCAGCCACCACTAACCTCATCAACATGAACAGCTTCACCTCAAATTTCGACATCGAGGCGGCCCCGTCTTCACAGAGCTTCATGGAGCCTCTTCAGCTTTCGAGGCCATCCCGAGATGATGAAGATGATGAGGAAGAGAGTCGAGTTCCACTCTTGTTCGCCAATGATCGCTATTGA

Protein Analysis

255

Amino Acids

27.11

Weight (kDa)

6.51

Isoelectric Point (pI)

59.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 57 - 155 2.8e-35 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015242)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03760
malus_domestica MD11G1229500.v1.1 MD12G1009400.v1.1 MD14G1006900.v1.1
prunus_persica Prupe.7G011300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0496241
rosa_laevigata RLG00000022544
rosa_multiflora Rmu_sc0004080.1_g000014 Rmu_ssc0000454.1_g000007
rosa_roxburghii Rroxscaffold_6G00389310
rosa_rugosa Rorug03G0281000
rosa_samantha Rh3AG327900 Rh3BG364100 Rh3CG361000 Rh3DG364000
rosa_wichuraiana Rw3G028720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 474
AciI CCGC 4 cut(s) 98, 210, 328, 647
AclWI GGATC 1 cut(s) 2
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 1 cut(s) 665
AdeI CACNNNGTG 2 cut(s) 173, 277
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 4 cut(s) 52, 69, 233, 392
AgsI TTSAA 1 cut(s) 325
AjiI CACGTC 1 cut(s) 36
AjnI CCWGG 1 cut(s) 549
AluBI AGCT 9 cut(s) 251, 266, 304, 371, 451, 472, 621, 666, 685
AluI AGCT 9 cut(s) 251, 266, 304, 371, 451, 472, 621, 666, 685
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 3 cut(s) 18, 301, 406
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 1 cut(s) 2
AlwNI CAGNNNCTG 1 cut(s) 550
Ama87I CYCGRG 1 cut(s) 699
AoxI GGCC 2 cut(s) 648, 692
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 8 cut(s) 164, 251, 263, 520, 523, 538, 541, 565
ApoI RAATTY 1 cut(s) 631
Asp700I GAANNNNTTC 1 cut(s) 620
AspS9I GGNCC 1 cut(s) 649
AsuHPI GGTGA 2 cut(s) 505, 616
AsuNHI GCTAGC 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 699
BaeGI GKGCMC 2 cut(s) 59, 273
BbsI GAAGAC 1 cut(s) 648
Bbv12I GWGCWC 1 cut(s) 273
BbvCI CCTCAGC 1 cut(s) 589
BbvI GCAGC 8 cut(s) 176, 238, 275, 532, 535, 550, 553, 577
BccI CCATC 1 cut(s) 703
BciT130I CCWGG 1 cut(s) 551
BcoDI GTCTC 3 cut(s) 18, 301, 406
BfaI CTAG 2 cut(s) 372, 452
BfuAI ACCTGC 1 cut(s) 474
Bme1390I CCNGG 1 cut(s) 551
BmeT110I CYCGRG 1 cut(s) 699
BmgBI CACGTC 1 cut(s) 36
BmgT120I GGNCC 1 cut(s) 649
BmiI GGNNCC 2 cut(s) 651, 675
BmrFI CCNGG 1 cut(s) 551
BmsI GCATC 1 cut(s) 443
BmtI GCTAGC 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 648
BplI GAGNNNNNCTC 2 cut(s) 723, 755
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 28, 244
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 69, 233, 392
Bse118I RCCGGY 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 551
BseDI CCNNGG 2 cut(s) 28, 244
BseGI GGATG 2 cut(s) 59, 695
BseLI CCNNNNNNNGG 4 cut(s) 52, 69, 233, 392
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 2 cut(s) 482, 603
BseRI GAGGAG 2 cut(s) 208, 577
BseSI GKGCMC 2 cut(s) 59, 273
BseXI GCAGC 8 cut(s) 176, 238, 275, 532, 535, 550, 553, 577
BsgI GTGCAG 2 cut(s) 183, 237
BshFI GGCC 2 cut(s) 650, 694
BsiHKAI GWGCWC 1 cut(s) 273
BsiHKCI CYCGRG 1 cut(s) 699
BsiSI CCGG 2 cut(s) 11, 331
BslI CCNNNNNNNGG 4 cut(s) 52, 69, 233, 392
BsmAI GTCTC 3 cut(s) 18, 301, 406
BsnI GGCC 2 cut(s) 650, 694
Bso31I GGTCTC 1 cut(s) 18
BsoBI CYCGRG 1 cut(s) 699
Bsp1286I GDGCHC 2 cut(s) 59, 273
Bsp143I GATC 2 cut(s) 7, 754
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 4 cut(s) 98, 210, 328, 647
BspANI GGCC 2 cut(s) 650, 694
BspCNI CTCAG 2 cut(s) 481, 602
BspLI GGNNCC 2 cut(s) 651, 675
BspMI ACCTGC 1 cut(s) 474
BspOI GCTAGC 1 cut(s) 455
BspPI GGATC 1 cut(s) 2
BspTNI GGTCTC 1 cut(s) 18
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 330
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 2 cut(s) 28, 244
BssMI GATC 2 cut(s) 7, 754
BssT1I CCWWGG 2 cut(s) 28, 244
Bst2UI CCWGG 1 cut(s) 551
Bst6I CTCTTC 2 cut(s) 684, 717
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 8 cut(s) 80, 84, 181, 256, 332, 444, 453, 570
BstDEI CTNAG 2 cut(s) 468, 589
BstDSI CCRYGG 1 cut(s) 28
BstF5I GGATG 2 cut(s) 59, 695
BstKTI GATC 2 cut(s) 10, 757
BstMAI GTCTC 3 cut(s) 18, 301, 406
BstMBI GATC 2 cut(s) 7, 754
BstNI CCWGG 1 cut(s) 551
BstSCI CCNGG 1 cut(s) 549
BstSLI GKGCMC 2 cut(s) 59, 273
BstV1I GCAGC 8 cut(s) 176, 238, 275, 532, 535, 550, 553, 577
BstV2I GAAGAC 1 cut(s) 648
BsuRI GGCC 2 cut(s) 650, 694
BtgI CCRYGG 1 cut(s) 28
BtrI CACGTC 1 cut(s) 36
BtsCI GGATG 2 cut(s) 59, 695
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 474
Cac8I GCNNGC 8 cut(s) 80, 84, 181, 256, 332, 444, 453, 570
CaiI CAGNNNCTG 1 cut(s) 550
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 649
CseI GACGC 2 cut(s) 75, 347
Csp6I GTAC 1 cut(s) 17
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 6 cut(s) 29, 171, 335, 533, 613, 670
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 2 cut(s) 468, 589
DpnI GATC 2 cut(s) 9, 756
DpnII GATC 2 cut(s) 7, 754
DraIII CACNNNGTG 2 cut(s) 173, 277
Eam1104I CTCTTC 2 cut(s) 684, 717
EarI CTCTTC 2 cut(s) 684, 717
Eco130I CCWWGG 2 cut(s) 28, 244
Eco31I GGTCTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 665
Eco88I CYCGRG 1 cut(s) 699
EcoRII CCWGG 1 cut(s) 549
EcoT14I CCWWGG 2 cut(s) 28, 244
ErhI CCWWGG 2 cut(s) 28, 244
FaeI CATG 6 cut(s) 32, 174, 338, 536, 616, 673
FatI CATG 6 cut(s) 28, 170, 334, 532, 612, 669
FauI CCCGC 1 cut(s) 203
FokI GGATG 2 cut(s) 46, 682
FspBI CTAG 2 cut(s) 372, 452
HaeIII GGCC 2 cut(s) 650, 694
HapII CCGG 2 cut(s) 11, 331
HgaI GACGC 2 cut(s) 75, 347
Hin1II CATG 6 cut(s) 32, 174, 338, 536, 616, 673
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 1 cut(s) 727
HpaII CCGG 2 cut(s) 11, 331
HphI GGTGA 2 cut(s) 505, 616
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 3 cut(s) 40, 241, 382
Hpy188III TCNNGA 2 cut(s) 298, 699
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 2 cut(s) 42, 46
HpyCH4IV ACGT 1 cut(s) 35
HpyF3I CTNAG 2 cut(s) 468, 589
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 6 cut(s) 32, 174, 338, 536, 616, 673
KroI GCCGGC 1 cut(s) 330
KroNI GCCGGC 1 cut(s) 332
Kzo9I GATC 2 cut(s) 7, 754
LmnI GCTCC 2 cut(s) 248, 673
LpnPI CCDG 8 cut(s) 24, 126, 344, 413, 469, 536, 563, 582
Lsp1109I GCAGC 8 cut(s) 176, 238, 275, 532, 535, 550, 553, 577
LweI GCATC 1 cut(s) 443
MaeI CTAG 2 cut(s) 372, 452
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 1 cut(s) 346
MalI GATC 2 cut(s) 9, 756
MboI GATC 2 cut(s) 7, 754
MboII GAAGA 4 cut(s) 648, 671, 722, 734
MhlI GDGCHC 2 cut(s) 59, 273
MluCI AATT 1 cut(s) 631
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 1 cut(s) 405
MroNI GCCGGC 1 cut(s) 330
MroXI GAANNNNTTC 1 cut(s) 620
MslI CAYNNNNRTG 1 cut(s) 611
MspA1I CMGCKG 2 cut(s) 266, 472
MspI CCGG 2 cut(s) 11, 331
MspR9I CCNGG 1 cut(s) 551
MvaI CCWGG 1 cut(s) 551
NaeI GCCGGC 1 cut(s) 332
NcoI CCATGG 1 cut(s) 28
NdeII GATC 2 cut(s) 7, 754
NgoMIV GCCGGC 1 cut(s) 330
NheI GCTAGC 1 cut(s) 451
NlaIII CATG 6 cut(s) 32, 174, 338, 536, 616, 673
NlaIV GGNNCC 2 cut(s) 651, 675
NmeAIII GCCGAG 1 cut(s) 472
PdiI GCCGGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 620
PflFI GACNNNGTC 1 cut(s) 341
PleI GAGTC 1 cut(s) 735
PpsI GAGTC 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 549
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 2 cut(s) 651, 675
PspPI GGNCC 1 cut(s) 649
PstNI CAGNNNCTG 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 341
PvuII CAGCTG 2 cut(s) 266, 472
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 611
Sau3AI GATC 2 cut(s) 7, 754
Sau96I GGNCC 1 cut(s) 649
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 1 cut(s) 551
SduI GDGCHC 2 cut(s) 59, 273
SfaNI GCATC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 1 cut(s) 631
SsiI CCGC 4 cut(s) 98, 210, 328, 647
SspMI CTAG 2 cut(s) 372, 452
StyD4I CCNGG 1 cut(s) 549
StyI CCWWGG 2 cut(s) 28, 244
TaiI ACGT 1 cut(s) 38
TaqI TCGA 5 cut(s) 299, 636, 642, 689, 730
TasI AATT 1 cut(s) 631
TatI WGTACW 1 cut(s) 16
TauI GCSGC 2 cut(s) 101, 650
TscAI CASTG 1 cut(s) 108
TseI GCWGC 8 cut(s) 164, 251, 263, 520, 523, 538, 541, 565
TspDTI ATGAA 3 cut(s) 629, 658, 723
TspRI CASTG 1 cut(s) 108
Tth111I GACNNNGTC 1 cut(s) 341
VneI GTGCAC 1 cut(s) 269
XapI RAATTY 1 cut(s) 631
XmnI GAANNNNTTC 1 cut(s) 620
XspI CTAG 2 cut(s) 372, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.