MD14G1006900.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015242
Physical Location & Seq
Forward (+)
743244 .. 744911
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1006900.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCTGATCAGCCAGTACAAGGAGATAATGGCAAGTCTGAGGGCCGAAGGAAGGGCACTTCCAGCGGCGGGGGAGGAAAGCGTGCTTCTATGAAGGCGGCAGTCAGAACGACAACAATAACTAGTAATGCAGTAGGTTCAGGTAGTGGTGCAGCTCCATGTGGGGCGTGCAAGTTTTTGAGGAGGAAGTGCATAAGCGGGTGCATATTCGCACCCCATTTCGGTTCAGACCAAGGAGCTGCACGCTTTGCCGCGGTGCACAAGGTGTTTGGGGCGAGTAATGTGTCGAAGCTTTTGTTGCACATTCCCTTGAACCGCCGGCATGACGCTGTCGTTACCATCACCTACGAGGCTCAAGCTAGGCTCTCCGACCCCGTTTACGGTTGTGTCTCCACCATACTTGCTTTGCAACAACAGGTTGCATCTTTGCAAGGCGAGCTAGCAATGGTGCAAACTCAGCTGTTAAACAGCAGGTTTGCAATTGCAAATGCTCTCCAAATCACTTCGCAGCAGCAGCAACAGCAACAGCACGAGCAGCAGCAAATGAACATGGCACTAATGCTACAGCCTGCTTACTCCAACAACACCTCCGCCTCCACTAACCTTATCAACATGAGCAGCTTGACCTCGAGCTTGGACATCGACGCGGCGCCATCTTCACAGAGCTTCATGGAGCCTCTTCAGCTTTCGCGGCCATCCCAAGATGATGAAGATGATGAGGAAGAGAGTCGAGTTCCCCTCTTGTTCGCCAATAATGGATATTAG

Protein Analysis

255

Amino Acids

27.14

Weight (kDa)

7.67

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 53 - 151 1.6e-34 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015242)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03760
malus_domestica MD11G1229500.v1.1 MD12G1009400.v1.1 MD14G1006900.v1.1
prunus_persica Prupe.7G011300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0496241
rosa_laevigata RLG00000022544
rosa_multiflora Rmu_sc0004080.1_g000014 Rmu_ssc0000454.1_g000007
rosa_roxburghii Rroxscaffold_6G00389310
rosa_rugosa Rorug03G0281000
rosa_samantha Rh3AG327900 Rh3BG364100 Rh3CG361000 Rh3DG364000
rosa_wichuraiana Rw3G028720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 462
AccB1I GGYRCC 1 cut(s) 649
AccII CGCG 3 cut(s) 254, 647, 691
AcoI YGGCCR 1 cut(s) 692
AcuI CTGAAG 1 cut(s) 665
AcyI GRCGYC 1 cut(s) 650
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 5 cut(s) 20, 52, 69, 221, 380
AgsI TTSAA 1 cut(s) 313
AhlI ACTAGT 1 cut(s) 122
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 394
Alw44I GTGCAC 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 2 cut(s) 43, 692
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 8 cut(s) 152, 239, 508, 511, 514, 535, 538, 618
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 334
AsuNHI GCTAGC 1 cut(s) 439
AvaI CYCGRG 1 cut(s) 628
BaeGI GKGCMC 2 cut(s) 59, 261
BanI GGYRCC 1 cut(s) 649
BauI CACGAG 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 8 cut(s) 164, 226, 520, 523, 526, 547, 550, 630
BccI CCATC 3 cut(s) 347, 661, 703
BclI TGATCA 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 394
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 3 cut(s) 123, 360, 440
BfmI CTRYAG 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 653
BfuAI ACCTGC 1 cut(s) 462
BmeT110I CYCGRG 1 cut(s) 628
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 2 cut(s) 651, 675
BmsI GCATC 1 cut(s) 431
BmtI GCTAGC 1 cut(s) 443
BplI GAGNNNNNCTC 2 cut(s) 723, 755
BpuEI CTTGAG 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 650
BsaJI CCNNGG 2 cut(s) 232, 252
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 52, 69, 221, 380
Bse118I RCCGGY 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 450
BseDI CCNNGG 2 cut(s) 232, 252
BseGI GGATG 1 cut(s) 695
BseLI CCNNNNNNNGG 5 cut(s) 20, 52, 69, 221, 380
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 2 cut(s) 30, 470
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 196
BseSI GKGCMC 2 cut(s) 59, 261
BseXI GCAGC 8 cut(s) 164, 226, 520, 523, 526, 547, 550, 630
BsgI GTGCAG 2 cut(s) 171, 225
Bsh1236I CGCG 3 cut(s) 254, 647, 691
BshFI GGCC 2 cut(s) 45, 694
BshNI GGYRCC 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 628
BsiSI CCGG 1 cut(s) 319
BslI CCNNNNNNNGG 5 cut(s) 20, 52, 69, 221, 380
BsmAI GTCTC 1 cut(s) 394
BsnI GGCC 2 cut(s) 45, 694
BsoBI CYCGRG 1 cut(s) 628
Bsp1286I GDGCHC 2 cut(s) 59, 261
Bsp143I GATC 1 cut(s) 7
BspANI GGCC 2 cut(s) 45, 694
BspCNI CTCAG 2 cut(s) 31, 469
BspFNI CGCG 3 cut(s) 254, 647, 691
BspLI GGNNCC 2 cut(s) 651, 675
BspMI ACCTGC 1 cut(s) 462
BspOI GCTAGC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 649
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 318
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 318
BssECI CCNNGG 2 cut(s) 232, 252
BssMI GATC 1 cut(s) 7
BssNI GRCGYC 1 cut(s) 650
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 530
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 2 cut(s) 684, 717
BstACI GRCGYC 1 cut(s) 650
BstAPI GCANNNNNTGC 1 cut(s) 248
BstC8I GCNNGC 7 cut(s) 84, 169, 244, 320, 437, 441, 570
BstDEI CTNAG 2 cut(s) 39, 456
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 695
BstFNI CGCG 3 cut(s) 254, 647, 691
BstH2I RGCGCY 1 cut(s) 653
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 1 cut(s) 10
BstMAI GTCTC 1 cut(s) 394
BstMBI GATC 1 cut(s) 7
BstSFI CTRYAG 1 cut(s) 563
BstSLI GKGCMC 2 cut(s) 59, 261
BstUI CGCG 3 cut(s) 254, 647, 691
BstV1I GCAGC 8 cut(s) 164, 226, 520, 523, 526, 547, 550, 630
BsuRI GGCC 2 cut(s) 45, 694
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 695
BveI ACCTGC 1 cut(s) 462
Cac8I GCNNGC 7 cut(s) 84, 169, 244, 320, 437, 441, 570
CfoI GCGC 1 cut(s) 652
Cfr10I RCCGGY 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 43
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 2 cut(s) 335, 653
Csp6I GTAC 1 cut(s) 17
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 5 cut(s) 159, 323, 550, 613, 670
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 2 cut(s) 39, 456
DinI GGCGCC 1 cut(s) 651
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
DraIII CACNNNGTG 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 692
Eam1104I CTCTTC 2 cut(s) 684, 717
EarI CTCTTC 2 cut(s) 684, 717
EciI GGCGGA 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 665
Eco88I CYCGRG 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 232
EgeI GGCGCC 1 cut(s) 651
EheI GGCGCC 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 232
FaeI CATG 5 cut(s) 162, 326, 553, 616, 673
FaiI YATR 9 cut(s) 92, 160, 194, 206, 324, 398, 551, 614, 671
FatI CATG 5 cut(s) 158, 322, 549, 612, 669
FauI CCCGC 2 cut(s) 62, 191
FbaI TGATCA 1 cut(s) 7
FokI GGATG 1 cut(s) 682
FspBI CTAG 3 cut(s) 123, 360, 440
GlaI GCGC 1 cut(s) 651
HaeII RGCGCY 1 cut(s) 653
HaeIII GGCC 2 cut(s) 45, 694
HapII CCGG 1 cut(s) 319
HgaI GACGC 2 cut(s) 335, 653
HhaI GCGC 1 cut(s) 652
Hin1I GRCGYC 1 cut(s) 650
Hin1II CATG 5 cut(s) 162, 326, 553, 616, 673
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 1 cut(s) 727
HpaII CCGG 1 cut(s) 319
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 2 cut(s) 259, 379
Hpy188I TCNGA 4 cut(s) 40, 107, 229, 370
Hpy8I GTNNAC 2 cut(s) 259, 379
Hpy99I CGWCG 1 cut(s) 647
HpyAV CCTTC 3 cut(s) 42, 46, 88
HpyCH4III ACNGT 1 cut(s) 383
HpyF3I CTNAG 2 cut(s) 39, 456
Hsp92I GRCGYC 1 cut(s) 650
Hsp92II CATG 5 cut(s) 162, 326, 553, 616, 673
HspAI GCGC 1 cut(s) 650
KasI GGCGCC 1 cut(s) 649
KroI GCCGGC 1 cut(s) 318
KroNI GCCGGC 1 cut(s) 320
Ksp22I TGATCA 1 cut(s) 7
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 1 cut(s) 7
LmnI GCTCC 3 cut(s) 160, 236, 673
LpnPI CCDG 7 cut(s) 27, 76, 126, 332, 401, 457, 582
Lsp1109I GCAGC 8 cut(s) 164, 226, 520, 523, 526, 547, 550, 630
LweI GCATC 1 cut(s) 431
MaeI CTAG 3 cut(s) 123, 360, 440
MaeIII GTNAC 1 cut(s) 334
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 4 cut(s) 648, 671, 722, 734
MfeI CAATTG 1 cut(s) 480
MhlI GDGCHC 2 cut(s) 59, 261
MluCI AATT 1 cut(s) 480
Mly113I GGCGCC 1 cut(s) 650
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 2 cut(s) 393, 603
MroNI GCCGGC 1 cut(s) 318
MseI TTAA 1 cut(s) 464
MspA1I CMGCKG 3 cut(s) 66, 254, 460
MspI CCGG 1 cut(s) 319
MunI CAATTG 1 cut(s) 480
MvnI CGCG 3 cut(s) 254, 647, 691
NaeI GCCGGC 1 cut(s) 320
NarI GGCGCC 1 cut(s) 650
NdeII GATC 1 cut(s) 7
NgoMIV GCCGGC 1 cut(s) 318
NheI GCTAGC 1 cut(s) 439
NlaIII CATG 5 cut(s) 162, 326, 553, 616, 673
NlaIV GGNNCC 2 cut(s) 651, 675
PaeR7I CTCGAG 1 cut(s) 628
PdiI GCCGGC 1 cut(s) 320
PflFI GACNNNGTC 1 cut(s) 329
PleI GAGTC 1 cut(s) 735
PluTI GGCGCC 1 cut(s) 653
PpsI GAGTC 1 cut(s) 735
PspN4I GGNNCC 2 cut(s) 651, 675
PspPI GGNCC 1 cut(s) 43
PspXI VCTCGAGB 1 cut(s) 628
PsyI GACNNNGTC 1 cut(s) 329
PvuII CAGCTG 1 cut(s) 460
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
SacII CCGCGG 1 cut(s) 255
SaqAI TTAA 1 cut(s) 464
Sau3AI GATC 1 cut(s) 7
Sau96I GGNCC 1 cut(s) 43
SchI GAGTC 1 cut(s) 736
SduI GDGCHC 2 cut(s) 59, 261
SfaNI GCATC 1 cut(s) 431
SfcI CTRYAG 1 cut(s) 563
SfoI GGCGCC 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 628
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SlaI CTCGAG 1 cut(s) 628
SmlI CTYRAG 2 cut(s) 354, 628
SmoI CTYRAG 2 cut(s) 354, 628
SpeI ACTAGT 1 cut(s) 122
Sse9I AATT 1 cut(s) 480
SspDI GGCGCC 1 cut(s) 649
SspMI CTAG 3 cut(s) 123, 360, 440
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 4 cut(s) 287, 629, 642, 730
TasI AATT 1 cut(s) 480
TatI WGTACW 1 cut(s) 16
TauI GCSGC 5 cut(s) 69, 101, 254, 650, 694
Tru1I TTAA 1 cut(s) 464
Tru9I TTAA 1 cut(s) 464
TseI GCWGC 8 cut(s) 152, 239, 508, 511, 514, 535, 538, 618
TspDTI ATGAA 4 cut(s) 107, 560, 658, 723
Tth111I GACNNNGTC 1 cut(s) 329
VneI GTGCAC 1 cut(s) 257
XhoI CTCGAG 1 cut(s) 628
XspI CTAG 3 cut(s) 123, 360, 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.