Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006763

Physical Location & Seq

Genomic Coordinates Forward (+)

1731491 .. 1738397

Gene Structure 6907 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1018100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3189 bp

ATGGAAATGGAGAGCTCGCGTAGGCCGTTTAGCAGATCGAATGAACCAGGCTTGAAGAAGCCCCGATTGGCCGAAAACCCGAATGGCCGAGCCTTTGGTCAGAGACCCGGTGGCGGAGCTAATCCAGTGCTGTCAAGGTTCAGAGTGAGTGACAGAGACGTGGGGAGCGATGATCCGAATCGCGCGGATGGGGGCTATGTGCCGCAGCCACTGCAGCATCAGGAGCTTGTGAGCCAGTACAAGACGGCGTTGGCGGAGCTCACCTTCAATTCAAAGCCAATTATTACCAATTTGACGATAATTGCCGGCGAGAATGTCCACGCGGCGAAGTCGGTGGCGGCAACTGTATGCGGCAACATTATTGAGGTTCCCAGTGAGCAGAAGTTACCATCACTTTATCTTTTGGACAGTATTGTAAAGAATATCGGAAGGGATTATATCAAATATTTTGCTGCCAGACTCCCTGAGGTATTCTGCAAGGCATATAGGCAGGTTGAACCTCCTATACATCAAAGCATGCGGCATCTTTTTGGAACATGGAAAGGAGTGTTTCCTGCTCAGACGCTTCAGATGATTGAGAAAGAACTCGGATTTTCTTCTACAGCAAATGGTTCATCCTCTGTAGCAGCAACATCAAGACCAGATTCGCAGTCACAACGACCAGCACATAGCATTCATGTGAATCCCAAATATTTGGAAAGGCAACGGCTTCAGCAGCCAGCAAGGGCAAAAGGAATGGCTAGCGACTTTTCTGGGGTTATGGCAAACTCAATTGATGATGATGCAGAGAGGCCAGCCAGAGCAGCAAGTATCAGTGCTGGACGACCATGGGTTGATCCTGCTGTTAAAATGCATCGTTCCAATACAGATGCACTTAGTGATCGTGTTCATGAGGAGAACATTGGTGCTGAATATGGAGAATACGAATATGGTTCAGATCTTCACAGGAATTCAAACCCGGGAATTGGAAGAATTGGTGGGAAGATTACAGAAACACTACCCACCCAAAGAAATGGTTTTAATATCAAGCATGGACTTACAAATTACTCGTCATCTAAATCTACGAATGCTGATCCTCGCTTAAAGACAGCACCAGGCATTGCAACTAGAAACAGTGGTGGCCTGTCTAGTAGCTGGAAGAATTCTGAGGAAGAGGAGTTCATGTGGGATGATATGAACTCAAGATTAACAGATCATGGCCCTCCAGATATTTCTAGTAACTCAAGAAAAGATCACTGGGCTTCTGATGATTCAGAGAAATTGGGCTTTGGAGGTCAGTTTCGTAAACCAAAAGGTGGAAATGATTATGCATTAACAGCTGATATGGATACCTCTGCTAATCCTACTGAACAGAAAGACCTATCTGCTCTTGGTCAGCGAATGTCATCACCTTGGACATTGCAGGACTCTATCGGTATTGACAGGCTGACCCCTTCTGGTACTCCTGTCATTAGTTCGGTTCATTCAGAGCGTTATGCTTCAAGTTTAAGTGGGCTATCGACAAGTGGAGATTCTTCTGTGGCCAGGATGGGAAGTAGAGCACAAGTAGCGTCTTCTCGAATTGGAACGTCAAGTCTAGGGTTTAATGCAGCAGCAGGGCCTACTGGAGCTTTAGGAAAACAACAGCTTCAGTCTGTGAGAGCTGCATCACCATCTGGACAGTCATCGATGCACCAATATTCTCCCTCACCTACATCTGCAGTACATCCCCGTCATCACTCGCAAAGTTTAGCTGACCAAGACTTTGCTGAGGAACCTTTGCCAATTCCAACTCCCAATGTTAGATTGGGCAGCAAAGCAAAATCCCAGCCTCAAGATCTATCTTCGTCAATTCCTGACATCCAATCAAGGCACAAATACCCCTTTCGACAACAACCAGACAGTATAGAGTCTGAATCTTTCGGTCACACTAAGAAGCCTCATGTGCCACAGGTCTCTACTTTTTCAACTCCCTCAACTGTTGGAGATTCTATACCAGATCTTTCAAATGTCCTTGCTGCAGAATCCTCAGGACAGTTGAACACAAGTAGTTTGTTGGCTGCTGTTATGAAGACTGGAATACTCTCTGACAAATCAATTACTTGTAGCCTGCCCAATTTGAATCGTCGGGACATGGGACATATTCCATCACAGTCTGGTGTTCAGCCTCCCTTGCAAAGTGGACCCTCCCCTACCCTTGCACTCCCAGGGCCCAAGGTTGCATCAGCACCTTCATCAGGTCACCTTTCTGGTGAGAACTCGCCAGCTTCCTCAAATGTATCACAGAAGAAGGTAGCACATCTGCCAATTCCACCTAGCCAACCTCCTTCCTCTCTTGATGGCAGTGCGTCAGCAAGTTCAACTGTGGTGAGTAATGCCTCAGATCCAATTTCAAACCTTTTAAGCTCCTTAGTTGCAAAGGGTTTGATATCTGCATCAAAGTCGGAGTCACCCACTCCTGTGCCATCCCAAAAGCTGACTGAACTGCACAACAAGGGCCTAGGTGCACCTGCCTCCAAATCAGTGTCAGTATCTCCAGTTTCAGTTTCAGTCTCTCCAAGTCGTCCTGTTACATCCCAAACTGATGCGTCTCTCCCAGAACCTGTAGCTAAAACATCTGCTGCCTTACCTCAATCTACCAAGACAGAAATACAAAACCTAATAGGCATTGCCTTTAAGCCAGATATAATCCGAGAATTTCATCCAGCTGTGATAGATGAACTTTTTAATGACCTTCCATATACGTGTAGCATATGTGGCCTTAGGCTCAAACTTAAAGAACGGCTTGAGAGACATTTGGAATGGCATGCTTTGAAAAATCCCGAAGGCAGTGGTTCAGTTATGGCATCAAGGAAGTGGTATCCAGATTTAACCAGCTGGGTTGCTGGAAGGACAGTACCCCCTTTGGTACCGGAGGCCATCAATTCAATAGACGAGCCTAATGAGATGATGGATATTGATGAGCCCATAGTTCCAGCAGACGAAAACCAATGTGCATGTGTTATATGTGGTGATATTTTTGAAGATTTTTACTGTCAAGAAAGGGATGAATGGATGTTCAAAGGAGCTTCGTACATGAGTATTCCATCTGCGGCCGGTAAGATGGAAACCACAGAGGAGAGTGTTTTGAAAGGTCCCATTGTGCATGCAAATTGTACAGTGGAAAGTTCACTCTCTGATAGCCATGTTAAATTGGAACCAGATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0000993 GO:0001098 GO:0001099 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005849 GO:0006139 GO:0006351 GO:0006353 GO:0006366 GO:0006369 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0019899 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0046483 GO:0070013 GO:0070063 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03015 ko04016 ko04075 map03015 map04016 map04075

Pfam Domains

Protein Families

CTD_bind

Protein Analysis

1063

Amino Acids

113.89

Weight (kDa)

6.38

Isoelectric Point (pI)

55.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CID	PF04818	81 - 190	1.8e-12	CID domain
zf_PCFS4	PF23228	980 - 1046	5.2e-29	PCFS4 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006763)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36480 AT2G36480
fragaria_vesca	FvH4_6g17910 FvH4_6g17910
malus_domestica	MD12G1018100.v1.1 MD14G1016100.v1.1
prunus_persica	Prupe.7G117000_v2.0.a1 Prupe.7G117000_v2.0.a1 Prupe.7G117000_v2.0.a1 Prupe.7G117000_v2.0.a1 Prupe.7G117000_v2.0.a1 Prupe.7G117000_v2.0.a1
pyrus_communis	pycom12g01510 pycom14g01390
rosa_chinensis	RchiOBHm_Chr3g0472161
rosa_laevigata	RLG00000024100
rosa_multiflora	Rmu_sc0002532.1_g000029 Rmu_sc0019091.1_g000001
rosa_roxburghii	Rroxscaffold_6G00409180
rosa_rugosa	Rorug03G0122700
rosa_samantha	Rh3AG174700 Rh3BG200000 Rh3CG191200 Rh3CG198500 Rh3DG174500
rosa_wichuraiana	Rw3G016630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2497
Acc36I	ACCTGC	2 cut(s)	481, 2497
Acc65I	GGTACC	1 cut(s)	2887
AccB1I	GGYRCC	1 cut(s)	2887
AccB7I	CCANNNNNTGG	1 cut(s)	1295
AccII	CGCG	4 cut(s)	19, 183, 185, 323
AciI	CCGC	9 cut(s)	114, 185, 203, 254, 323, 338, 351, 520, 3071
AclWI	GGATC	4 cut(s)	167, 830, 1067, 2357
AcoI	YGGCCR	4 cut(s)	69, 85, 1521, 3072
AcsI	RAATTY	3 cut(s)	949, 1141, 2675
AcuI	CTGAAG	3 cut(s)	551, 695, 1613
AdeI	CACNNNGTG	1 cut(s)	878
AfaI	GTAC	7 cut(s)	239, 1441, 1704, 2877, 2889, 3053, 3136
AfiI	CCNNNNNNNGG	4 cut(s)	113, 965, 1295, 2216
AflIII	ACRYGT	1 cut(s)	2723
AjiI	CACGTC	1 cut(s)	160
AjnI	CCWGG	4 cut(s)	46, 1093, 1523, 2185
AjuI	GAANNNNNNNTTGG	2 cut(s)	50, 82
AleI	CACNNNNGTG	1 cut(s)	2438
AloI	GAACNNNNNNTCC	2 cut(s)	1142, 1174
Alw21I	GWGCWC	4 cut(s)	17, 261, 1543, 2488
Alw26I	GTCTC	6 cut(s)	97, 150, 1939, 2536, 2574, 2764
Alw44I	GTGCAC	1 cut(s)	2484
AlwI	GGATC	4 cut(s)	167, 830, 1067, 2357
AlwNI	CAGNNNCTG	2 cut(s)	211, 2582
Ama87I	CYCGRG	1 cut(s)	958
ApaI	GGGCCC	1 cut(s)	2193
ApaLI	GTGCAC	1 cut(s)	2484
ApoI	RAATTY	3 cut(s)	949, 1141, 2675
ArsI	GACNNNNNNTTYG	6 cut(s)	1034, 1066, 1727, 1759, 2411, 2443
Asp718I	GGTACC	1 cut(s)	2887
AspA2I	CCTAGG	1 cut(s)	2479
AspLEI	GCGC	1 cut(s)	185
AspS9I	GGNCC	7 cut(s)	1199, 1598, 2162, 2189, 2190, 2476, 3113
AsuC2I	CCSGG	3 cut(s)	108, 959, 960
AsuHPI	GGTGA	9 cut(s)	253, 1380, 1641, 1680, 2213, 2243, 2359, 2421, 3002
AsuNHI	GCTAGC	1 cut(s)	740
AvaI	CYCGRG	1 cut(s)	958
AvaII	GGWCC	2 cut(s)	2162, 3113
AvrII	CCTAGG	1 cut(s)	2479
AxyI	CCTNAGG	3 cut(s)	465, 2008, 2741
BaeGI	GKGCMC	2 cut(s)	2193, 2488
BaeI	ACNNNNGTAYC	2 cut(s)	1431, 1464
BalI	TGGCCA	1 cut(s)	1523
BanI	GGYRCC	1 cut(s)	2887
BanII	GRGCYC	4 cut(s)	17, 261, 2193, 2946
BbsI	GAAGAC	2 cut(s)	1545, 2057
Bbv12I	GWGCWC	4 cut(s)	17, 261, 1543, 2488
BbvCI	CCTCAGC	1 cut(s)	1749
BceAI	ACGGC	4 cut(s)	10, 261, 722, 2777
BcgI	CGANNNNNNTGC	2 cut(s)	2403, 2437
BciT130I	CCWGG	4 cut(s)	48, 1095, 1525, 2187
BciVI	GTATCC	2 cut(s)	1321, 2850
BcnI	CCSGG	3 cut(s)	108, 959, 960
BcoDI	GTCTC	6 cut(s)	97, 150, 1939, 2536, 2574, 2764
BfaI	CTAG	7 cut(s)	741, 1107, 1128, 1215, 1577, 2295, 2480
BfmI	CTRYAG	6 cut(s)	212, 600, 621, 1698, 1998, 2583
BfuAI	ACCTGC	2 cut(s)	481, 2497
BfuI	GTATCC	2 cut(s)	1321, 2850
BglII	AGATCT	3 cut(s)	937, 1816, 1978
BlnI	CCTAGG	1 cut(s)	2479
Bme1390I	CCNGG	7 cut(s)	48, 108, 959, 960, 1095, 1525, 2187
Bme18I	GGWCC	2 cut(s)	2162, 3113
BmeT110I	CYCGRG	1 cut(s)	958
BmgBI	CACGTC	1 cut(s)	160
BmgT120I	GGNCC	7 cut(s)	1199, 1598, 2162, 2189, 2190, 2476, 3113
BmiI	GGNNCC	7 cut(s)	369, 1755, 2164, 2191, 2889, 3115, 3177
BmrFI	CCNGG	7 cut(s)	48, 108, 959, 960, 1095, 1525, 2187
BmrI	ACTGGG	2 cut(s)	366, 1246
BmtI	GCTAGC	1 cut(s)	744
BmuI	ACTGGG	2 cut(s)	366, 1246
BpiI	GAAGAC	2 cut(s)	1545, 2057
BpmI	CTGGAG	3 cut(s)	1188, 1626, 2499
Bpu10I	CCTNAGC	1 cut(s)	1749
BpuEI	CTTGAG	4 cut(s)	1165, 1207, 1797, 2786
BpuMI	CCSGG	3 cut(s)	108, 959, 960
Bsa29I	ATCGAT	1 cut(s)	1667
BsaAI	YACGTR	1 cut(s)	2724
BsaBI	GATNNNNATC	1 cut(s)	177
BsaI	GGTCTC	2 cut(s)	97, 1939
BsaJI	CCNNGG	7 cut(s)	827, 958, 1391, 2185, 2186, 2193, 2479
BsaWI	WCCGGW	1 cut(s)	2890
BsaXI	ACNNNNNCTCC	2 cut(s)	1148, 1178
Bsc4I	CCNNNNNNNGG	4 cut(s)	113, 965, 1295, 2216
Bse118I	RCCGGY	2 cut(s)	305, 3074
Bse1I	ACTGG	7 cut(s)	125, 235, 372, 1241, 1609, 2059, 2516
Bse21I	CCTNAGG	3 cut(s)	465, 2008, 2741
Bse3DI	GCAATG	3 cut(s)	1098, 1397, 2646
Bse8I	GATNNNNATC	1 cut(s)	177
BseBI	CCWGG	4 cut(s)	48, 1095, 1525, 2187
BseCI	ATCGAT	1 cut(s)	1667
BseDI	CCNNGG	7 cut(s)	827, 958, 1391, 2185, 2186, 2193, 2479
BseJI	GATNNNNATC	1 cut(s)	177
BseLI	CCNNNNNNNGG	4 cut(s)	113, 965, 1295, 2216
BseMI	GCAATG	3 cut(s)	1098, 1397, 2646
BseMII	CTCAG	6 cut(s)	456, 572, 1137, 1740, 2022, 2373
BseNI	ACTGG	7 cut(s)	125, 235, 372, 1241, 1609, 2059, 2516
BseRI	GAGGAG	3 cut(s)	908, 1169, 3110
BseSI	GKGCMC	2 cut(s)	2193, 2488
BseX3I	CGGCCG	1 cut(s)	3072
BseYI	CCCAGC	2 cut(s)	1806, 2856
BsgI	GTGCAG	1 cut(s)	2450
Bsh1236I	CGCG	4 cut(s)	19, 183, 185, 323
Bsh1285I	CGRYCG	1 cut(s)	3075
BshNI	GGYRCC	1 cut(s)	2887
BshVI	ATCGAT	1 cut(s)	1667
BsiEI	CGRYCG	1 cut(s)	3075
BsiHKAI	GWGCWC	4 cut(s)	17, 261, 1543, 2488
BsiHKCI	CYCGRG	1 cut(s)	958
BsiSI	CCGG	5 cut(s)	108, 306, 959, 2891, 3075
BslFI	GGGAC	3 cut(s)	2123, 2130, 3099
BslI	CCNNNNNNNGG	4 cut(s)	113, 965, 1295, 2216
BsmAI	GTCTC	6 cut(s)	97, 150, 1939, 2536, 2574, 2764
BsmBI	CGTCTC	2 cut(s)	150, 2574
BsmFI	GGGAC	3 cut(s)	2123, 2130, 3099
BsmI	GAATGC	2 cut(s)	672, 1072
Bso31I	GGTCTC	2 cut(s)	97, 1939
BsoBI	CYCGRG	1 cut(s)	958
Bsp120I	GGGCCC	1 cut(s)	2189
Bsp1286I	GDGCHC	6 cut(s)	17, 261, 1543, 2193, 2488, 2946
Bsp1407I	TGTACA	1 cut(s)	3134
Bsp19I	CCATGG	1 cut(s)	827
BspACI	CCGC	9 cut(s)	114, 185, 203, 254, 323, 338, 351, 520, 3071
BspCNI	CTCAG	6 cut(s)	457, 571, 1138, 1741, 2021, 2372
BspDI	ATCGAT	1 cut(s)	1667
BspFNI	CGCG	4 cut(s)	19, 183, 185, 323
BspHI	TCATGA	1 cut(s)	889
BspLI	GGNNCC	7 cut(s)	369, 1755, 2164, 2191, 2889, 3115, 3177
BspMAI	CTGCAG	3 cut(s)	216, 1702, 2002
BspMI	ACCTGC	2 cut(s)	481, 2497
BspOI	GCTAGC	1 cut(s)	744
BspPI	GGATC	4 cut(s)	167, 830, 1067, 2357
BspT107I	GGYRCC	1 cut(s)	2887
BspTNI	GGTCTC	2 cut(s)	97, 1939
BsrDI	GCAATG	3 cut(s)	1098, 1397, 2646
BsrFI	RCCGGY	2 cut(s)	305, 3074
BsrGI	TGTACA	1 cut(s)	3134
BsrI	ACTGG	7 cut(s)	125, 235, 372, 1241, 1609, 2059, 2516
BssAI	RCCGGY	2 cut(s)	305, 3074
BssECI	CCNNGG	7 cut(s)	827, 958, 1391, 2185, 2186, 2193, 2479
BssT1I	CCWWGG	4 cut(s)	827, 1391, 2193, 2479
Bst2UI	CCWGG	4 cut(s)	48, 1095, 1525, 2187
Bst6I	CTCTTC	1 cut(s)	1146
BstAPI	GCANNNNNTGC	1 cut(s)	211
BstAUI	TGTACA	1 cut(s)	3134
BstBAI	YACGTR	1 cut(s)	2724
BstDSI	CCRYGG	1 cut(s)	827
BstEII	GGTNACC	1 cut(s)	2219
BstENI	CCTNNNNNAGG	1 cut(s)	2214
BstFNI	CGCG	4 cut(s)	19, 183, 185, 323
BstHHI	GCGC	1 cut(s)	185
BstMAI	GTCTC	6 cut(s)	97, 150, 1939, 2536, 2574, 2764
BstMCI	CGRYCG	1 cut(s)	3075
BstNI	CCWGG	4 cut(s)	48, 1095, 1525, 2187
BstNSI	RCATGY	4 cut(s)	520, 2789, 2979, 3128
BstPI	GGTNACC	1 cut(s)	2219
BstSCI	CCNGG	7 cut(s)	46, 106, 957, 958, 1093, 1523, 2185
BstSFI	CTRYAG	6 cut(s)	212, 600, 621, 1698, 1998, 2583
BstSLI	GKGCMC	2 cut(s)	2193, 2488
BstUI	CGCG	4 cut(s)	19, 183, 185, 323
BstV2I	GAAGAC	2 cut(s)	1545, 2057
BstX2I	RGATCY	4 cut(s)	937, 1816, 1978, 2362
BstXI	CCANNNNNNTGG	2 cut(s)	694, 1013
BstYI	RGATCY	4 cut(s)	937, 1816, 1978, 2362
BstZI	CGGCCG	1 cut(s)	3072
Bsu15I	ATCGAT	1 cut(s)	1667
Bsu36I	CCTNAGG	3 cut(s)	465, 2008, 2741
BsuI	GTATCC	2 cut(s)	1321, 2850
BsuTUI	ATCGAT	1 cut(s)	1667
BtgI	CCRYGG	1 cut(s)	827
BtgZI	GCGATG	1 cut(s)	183
BtrI	CACGTC	1 cut(s)	160
BtsI	GCAGTG	3 cut(s)	209, 2329, 2815
BveI	ACCTGC	2 cut(s)	481, 2497
CaiI	CAGNNNCTG	2 cut(s)	211, 2582
CciI	TCATGA	1 cut(s)	889
CfoI	GCGC	1 cut(s)	185
Cfr10I	RCCGGY	2 cut(s)	305, 3074
Cfr13I	GGNCC	7 cut(s)	1199, 1598, 2162, 2189, 2190, 2476, 3113
Cfr9I	CCCGGG	1 cut(s)	958
ClaI	ATCGAT	1 cut(s)	1667
CseI	GACGC	4 cut(s)	571, 1539, 2316, 2556
Csp6I	GTAC	7 cut(s)	238, 1440, 1703, 2876, 2888, 3052, 3135
CviQI	GTAC	7 cut(s)	238, 1440, 1703, 2876, 2888, 3052, 3135
DraIII	CACNNNGTG	1 cut(s)	878
EaeI	YGGCCR	4 cut(s)	69, 85, 1521, 3072
EagI	CGGCCG	1 cut(s)	3072
Eam1104I	CTCTTC	1 cut(s)	1146
EarI	CTCTTC	1 cut(s)	1146
EciI	GGCGGA	2 cut(s)	129, 269
Ecl136II	GAGCTC	2 cut(s)	15, 259
EclXI	CGGCCG	1 cut(s)	3072
Eco130I	CCWWGG	4 cut(s)	827, 1391, 2193, 2479
Eco24I	GRGCYC	4 cut(s)	17, 261, 2193, 2946
Eco31I	GGTCTC	2 cut(s)	97, 1939
Eco32I	GATATC	1 cut(s)	2409
Eco47I	GGWCC	2 cut(s)	2162, 3113
Eco52I	CGGCCG	1 cut(s)	3072
Eco53kI	GAGCTC	2 cut(s)	15, 259
Eco57I	CTGAAG	3 cut(s)	551, 695, 1613
Eco81I	CCTNAGG	3 cut(s)	465, 2008, 2741
Eco88I	CYCGRG	1 cut(s)	958
Eco91I	GGTNACC	1 cut(s)	2219
EcoICRI	GAGCTC	2 cut(s)	15, 259
EcoNI	CCTNNNNNAGG	1 cut(s)	2214
EcoO109I	RGGNCCY	4 cut(s)	1598, 2189, 2476, 3113
EcoO65I	GGTNACC	1 cut(s)	2219
EcoRI	GAATTC	2 cut(s)	949, 1141
EcoRII	CCWGG	4 cut(s)	46, 1093, 1523, 2185
EcoRV	GATATC	1 cut(s)	2409
EcoT14I	CCWWGG	4 cut(s)	827, 1391, 2193, 2479
EcoT22I	ATGCAT	2 cut(s)	855, 1312
EcoT38I	GRGCYC	4 cut(s)	17, 261, 2193, 2946
ErhI	CCWWGG	4 cut(s)	827, 1391, 2193, 2479
Esp3I	CGTCTC	2 cut(s)	150, 2574
FalI	AAGNNNNNCTT	2 cut(s)	2748, 2780
FaqI	GGGAC	3 cut(s)	2123, 2130, 3099
FauNDI	CATATG	1 cut(s)	2732
FriOI	GRGCYC	4 cut(s)	17, 261, 2193, 2946
FspBI	CTAG	7 cut(s)	741, 1107, 1128, 1215, 1577, 2295, 2480
GlaI	GCGC	1 cut(s)	184
GsaI	CCCAGC	2 cut(s)	1810, 2860
GsuI	CTGGAG	3 cut(s)	1188, 1626, 2499
HapII	CCGG	5 cut(s)	108, 306, 959, 2891, 3075
HgaI	GACGC	4 cut(s)	571, 1539, 2316, 2556
HhaI	GCGC	1 cut(s)	185
Hin6I	GCGC	1 cut(s)	183
HinP1I	GCGC	1 cut(s)	183
HpaII	CCGG	5 cut(s)	108, 306, 959, 2891, 3075
HphI	GGTGA	9 cut(s)	253, 1380, 1641, 1680, 2213, 2243, 2359, 2421, 3002
Hpy166II	GTNNAC	5 cut(s)	319, 1286, 2162, 2486, 3149
Hpy8I	GTNNAC	5 cut(s)	319, 1286, 2162, 2486, 3149
Hpy99I	CGWCG	1 cut(s)	2109
HpyAV	CCTTC	9 cut(s)	274, 423, 1443, 2220, 2263, 2316, 2723, 2798, 2862
HpyCH4IV	ACGT	3 cut(s)	159, 1568, 2723
HpySE526I	ACGT	3 cut(s)	159, 1568, 2723
HspAI	GCGC	1 cut(s)	183
KpnI	GGTACC	1 cut(s)	2891
KroI	GCCGGC	1 cut(s)	305
KroNI	GCCGGC	1 cut(s)	307
LmnI	GCTCC	7 cut(s)	116, 165, 223, 256, 1607, 2390, 3044
MaeI	CTAG	7 cut(s)	741, 1107, 1128, 1215, 1577, 2295, 2480
MaeII	ACGT	3 cut(s)	159, 1568, 2723
MaeIII	GTNAC	8 cut(s)	149, 384, 651, 1217, 1904, 2219, 2427, 2548
MfeI	CAATTG	1 cut(s)	771
MflI	RGATCY	4 cut(s)	937, 1816, 1978, 2362
MhlI	GDGCHC	6 cut(s)	17, 261, 1543, 2193, 2488, 2946
MlsI	TGGCCA	1 cut(s)	1523
MluNI	TGGCCA	1 cut(s)	1523
MlyI	GAGTC	4 cut(s)	453, 1400, 1898, 2435
MmeI	TCCRAC	3 cut(s)	1793, 1942, 2403
Mox20I	TGGCCA	1 cut(s)	1523
Mph1103I	ATGCAT	2 cut(s)	855, 1312
MroNI	GCCGGC	1 cut(s)	305
MscI	TGGCCA	1 cut(s)	1523
MslI	CAYNNNNRTG	3 cut(s)	677, 2438, 2722
Msp20I	TGGCCA	1 cut(s)	1523
MspA1I	CMGCKG	3 cut(s)	1319, 2687, 2856
MspI	CCGG	5 cut(s)	108, 306, 959, 2891, 3075
MspR9I	CCNGG	7 cut(s)	48, 108, 959, 960, 1095, 1525, 2187
MunI	CAATTG	1 cut(s)	771
Mva1269I	GAATGC	2 cut(s)	672, 1072
MvaI	CCWGG	4 cut(s)	48, 1095, 1525, 2187
MvnI	CGCG	4 cut(s)	19, 183, 185, 323
NaeI	GCCGGC	1 cut(s)	307
NciI	CCSGG	3 cut(s)	108, 959, 960
NcoI	CCATGG	1 cut(s)	827
NdeI	CATATG	1 cut(s)	2732
NgoMIV	GCCGGC	1 cut(s)	305
NheI	GCTAGC	1 cut(s)	740
NlaIV	GGNNCC	7 cut(s)	369, 1755, 2164, 2191, 2889, 3115, 3177
NmeAIII	GCCGAG	1 cut(s)	113
NmuCI	GTSAC	5 cut(s)	149, 651, 1904, 2219, 2427
NsiI	ATGCAT	2 cut(s)	855, 1312
NspI	RCATGY	4 cut(s)	520, 2789, 2979, 3128
OliI	CACNNNNGTG	1 cut(s)	2438
PaeI	GCATGC	3 cut(s)	520, 2789, 3128
PagI	TCATGA	1 cut(s)	889
PaqCI	CACCTGC	1 cut(s)	2497
PasI	CCCWGGG	1 cut(s)	2186
PcsI	WCGNNNNNNNCGW	2 cut(s)	23, 165
PctI	GAATGC	2 cut(s)	672, 1072
PdiI	GCCGGC	1 cut(s)	307
PfeI	GAWTC	9 cut(s)	178, 644, 682, 1250, 1511, 1895, 1967, 2003, 2101
PflMI	CCANNNNNTGG	1 cut(s)	1295
PleI	GAGTC	4 cut(s)	453, 1400, 1897, 2434
PpsI	GAGTC	4 cut(s)	453, 1400, 1897, 2434
Ppu21I	YACGTR	1 cut(s)	2724
PpuMI	RGGWCCY	1 cut(s)	3113
Psp124BI	GAGCTC	2 cut(s)	17, 261
Psp5II	RGGWCCY	1 cut(s)	3113
Psp6I	CCWGG	4 cut(s)	46, 1093, 1523, 2185
PspEI	GGTNACC	1 cut(s)	2219
PspFI	CCCAGC	2 cut(s)	1806, 2856
PspGI	CCWGG	4 cut(s)	46, 1093, 1523, 2185
PspN4I	GGNNCC	7 cut(s)	369, 1755, 2164, 2191, 2889, 3115, 3177
PspOMI	GGGCCC	1 cut(s)	2189
PspPI	GGNCC	7 cut(s)	1199, 1598, 2162, 2189, 2190, 2476, 3113
PspPPI	RGGWCCY	1 cut(s)	3113
PsrI	GAACNNNNNNTAC	2 cut(s)	489, 521
PstI	CTGCAG	3 cut(s)	216, 1702, 2002
PstNI	CAGNNNCTG	2 cut(s)	211, 2582
PsuI	RGATCY	4 cut(s)	937, 1816, 1978, 2362
PvuII	CAGCTG	3 cut(s)	1319, 2687, 2856
RsaI	GTAC	7 cut(s)	239, 1441, 1704, 2877, 2889, 3053, 3136
RsaNI	GTAC	7 cut(s)	238, 1440, 1703, 2876, 2888, 3052, 3135
RseI	CAYNNNNRTG	3 cut(s)	677, 2438, 2722
SacI	GAGCTC	2 cut(s)	17, 261
Sau96I	GGNCC	7 cut(s)	1199, 1598, 2162, 2189, 2190, 2476, 3113
SchI	GAGTC	4 cut(s)	453, 1400, 1898, 2435
ScrFI	CCNGG	7 cut(s)	48, 108, 959, 960, 1095, 1525, 2187
SduI	GDGCHC	6 cut(s)	17, 261, 1543, 2193, 2488, 2946
SfcI	CTRYAG	6 cut(s)	212, 600, 621, 1698, 1998, 2583
SinI	GGWCC	2 cut(s)	2162, 3113
SmaI	CCCGGG	1 cut(s)	960
SmiMI	CAYNNNNRTG	3 cut(s)	677, 2438, 2722
SmlI	CTYRAG	4 cut(s)	1180, 1222, 1812, 2765
SmoI	CTYRAG	4 cut(s)	1180, 1222, 1812, 2765
SphI	GCATGC	3 cut(s)	520, 2789, 3128
SsiI	CCGC	9 cut(s)	114, 185, 203, 254, 323, 338, 351, 520, 3071
SspI	AATATT	3 cut(s)	446, 692, 1679
SspMI	CTAG	7 cut(s)	741, 1107, 1128, 1215, 1577, 2295, 2480
SstI	GAGCTC	2 cut(s)	17, 261
StyD4I	CCNGG	7 cut(s)	46, 106, 957, 958, 1093, 1523, 2185
StyI	CCWWGG	4 cut(s)	827, 1391, 2193, 2479
TaiI	ACGT	3 cut(s)	162, 1571, 2726
TaqI	TCGA	5 cut(s)	38, 1499, 1558, 1667, 1867
TaqII	GACCGA	1 cut(s)	1892
TatI	WGTACW	3 cut(s)	237, 1702, 3134
TauI	GCSGC	6 cut(s)	205, 326, 341, 354, 523, 3074
TfiI	GAWTC	9 cut(s)	178, 644, 682, 1250, 1511, 1895, 1967, 2003, 2101
TseFI	GTSAC	5 cut(s)	149, 651, 1904, 2219, 2427
Tsp45I	GTSAC	5 cut(s)	149, 651, 1904, 2219, 2427
TspMI	CCCGGG	1 cut(s)	958
Van91I	CCANNNNNTGG	1 cut(s)	1295
VneI	GTGCAC	1 cut(s)	2484
VpaK11BI	GGWCC	2 cut(s)	2162, 3113
XagI	CCTNNNNNAGG	1 cut(s)	2214
XapI	RAATTY	3 cut(s)	949, 1141, 2675
XceI	RCATGY	4 cut(s)	520, 2789, 2979, 3128
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1783
XmaI	CCCGGG	1 cut(s)	958
XmaJI	CCTAGG	1 cut(s)	2479
XspI	CTAG	7 cut(s)	741, 1107, 1128, 1215, 1577, 2295, 2480
Zsp2I	ATGCAT	2 cut(s)	855, 1312

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.