MD12G1029300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013540
Physical Location & Seq
Reverse (-)
3222124 .. 3223389
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1029300.v1.1.491

Sequence Viewer

Length: 246 bp
ATGCCTTTGAAGGTAGACAAAATTGCAGGTGGGACTAGATTGGGGTTTGTTACAATCGGGAATGCGGAGGTTGAGGTCCCTGTGTACATAGACTGCTTGGTTTTCCCACCAAGTGACGGCTCTGGCCCTATTTTCCGAGCTGTCAGAAGCGGACCCTTGAAGGACAAGACACCTCCTGGTGAACCAAGGATCATGAGAAATCTTATGCAGGCCCTTCAGAAGTCGGTCCAAATCGCTAGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

82

Amino Acids

8.76

Weight (kDa)

9.74

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7148 PF23650 1 - 80 9.1e-35 Domain of unknown function (DUF7148)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36145
fragaria_vesca FvH4_6g19360
malus_domestica MD12G1029300.v1.1 MD12G1029500.v1.1 MD14G1029600.v1.1 MD14G1029800.v1.1
prunus_persica Prupe.7G102300_v2.0.a1
pyrus_communis pycom14g02750
rosa_chinensis RchiOBHm_Chr3g0474621
rosa_laevigata RLG00000023907
rosa_multiflora Rmu_sc0001353.1_g000039 Rmu_sc0001353.1_g000048
rosa_roxburghii Rroxscaffold_6G00407040
rosa_rugosa Rorug03G0142200
rosa_samantha Rh3AG192500 Rh3BG222400 Rh3CG217100 Rh3DG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 15
AciI CCGC 2 cut(s) 65, 150
AclWI GGATC 1 cut(s) 197
AcuI CTGAAG 1 cut(s) 200
AdeI CACNNNGTG 1 cut(s) 113
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 2 cut(s) 10, 160
AjnI CCWGG 1 cut(s) 175
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
AlwI GGATC 1 cut(s) 197
AoxI GGCC 2 cut(s) 124, 210
AspS9I GGNCC 5 cut(s) 76, 125, 152, 211, 226
AsuHPI GGTGA 1 cut(s) 191
AvaII GGWCC 3 cut(s) 76, 152, 226
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 177
BfaI CTAG 2 cut(s) 36, 237
BfuAI ACCTGC 1 cut(s) 17
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 3 cut(s) 76, 152, 226
BmgT120I GGNCC 5 cut(s) 76, 125, 152, 211, 226
BmiI GGNNCC 2 cut(s) 78, 154
BmrFI CCNGG 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 185
Bsc4I CCNNNNNNNGG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 185
BseLI CCNNNNNNNGG 1 cut(s) 116
BshFI GGCC 2 cut(s) 126, 212
BslFI GGGAC 2 cut(s) 46, 62
BslI CCNNNNNNNGG 1 cut(s) 116
BsmFI GGGAC 2 cut(s) 46, 62
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 2 cut(s) 126, 212
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 2 cut(s) 65, 150
BspANI GGCC 2 cut(s) 126, 212
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 78, 154
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 185
BssMI GATC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 1 cut(s) 243
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 210
BstKTI GATC 1 cut(s) 192
BstMBI GATC 1 cut(s) 189
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 175
BsuRI GGCC 2 cut(s) 126, 212
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 210
CciI TCATGA 1 cut(s) 192
Cfr13I GGNCC 5 cut(s) 76, 125, 152, 211, 226
Csp6I GTAC 1 cut(s) 85
CviAII CATG 1 cut(s) 193
CviJI RGCY 4 cut(s) 120, 126, 140, 212
CviKI_1 RGCY 4 cut(s) 120, 126, 140, 212
CviQI GTAC 1 cut(s) 85
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
DraIII CACNNNGTG 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 185
Eco47I GGWCC 3 cut(s) 76, 152, 226
Eco57I CTGAAG 1 cut(s) 200
EcoO109I RGGNCCY 2 cut(s) 76, 211
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
FaeI CATG 1 cut(s) 196
FaiI YATR 3 cut(s) 89, 194, 206
FaqI GGGAC 2 cut(s) 46, 62
FatI CATG 1 cut(s) 192
FblI GTMKAC 1 cut(s) 15
FspBI CTAG 2 cut(s) 36, 237
HaeIII GGCC 2 cut(s) 126, 212
Hin1II CATG 1 cut(s) 196
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 3 cut(s) 16, 85, 182
Hpy188I TCNGA 3 cut(s) 137, 146, 219
Hpy188III TCNNGA 2 cut(s) 58, 193
Hpy8I GTNNAC 3 cut(s) 16, 85, 182
HpyAV CCTTC 3 cut(s) 4, 154, 224
HpyCH4III ACNGT 1 cut(s) 243
HpyCH4V TGCA 2 cut(s) 26, 208
Hsp92II CATG 1 cut(s) 196
Kzo9I GATC 1 cut(s) 189
LpnPI CCDG 6 cut(s) 12, 93, 108, 162, 189, 194
MaeI CTAG 2 cut(s) 36, 237
MaeIII GTNAC 2 cut(s) 49, 113
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MluCI AATT 1 cut(s) 21
MnlI CCTC 3 cut(s) 61, 67, 183
MspR9I CCNGG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 1 cut(s) 177
NdeII GATC 1 cut(s) 189
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 2 cut(s) 78, 154
NmuCI GTSAC 1 cut(s) 113
PagI TCATGA 1 cut(s) 192
PaqCI CACCTGC 1 cut(s) 17
PctI GAATGC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 76
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 2 cut(s) 78, 154
PspPI GGNCC 5 cut(s) 76, 125, 152, 211, 226
PspPPI RGGWCCY 1 cut(s) 76
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 5 cut(s) 76, 125, 152, 211, 226
ScrFI CCNGG 1 cut(s) 177
SetI ASST 6 cut(s) 15, 31, 72, 78, 142, 175
SinI GGWCC 3 cut(s) 76, 152, 226
Sse9I AATT 1 cut(s) 21
SsiI CCGC 2 cut(s) 65, 150
SspMI CTAG 2 cut(s) 36, 237
StyD4I CCNGG 1 cut(s) 175
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 1 cut(s) 243
TaqII GACCGA 1 cut(s) 214
TasI AATT 1 cut(s) 21
TatI WGTACW 1 cut(s) 84
TseFI GTSAC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 113
VpaK11BI GGWCC 3 cut(s) 76, 152, 226
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 2 cut(s) 36, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.