pycom14g02750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013540
Physical Location & Seq
Reverse (-)
2096142 .. 2096453
312 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g02750.1

Sequence Viewer

Length: 312 bp
ATGGACACATTGGAATATATTATGACAATGGCTTTGAGACCTAGCAATGGACTGAATATTTTCTTGTACATTGCGATGGAACAGGCGGACAAAATTGCAGGTGGGACTAGATTGGGTTTTGTTACAATTGGGAATGCGGAGGTTGAGGTCCCTGTGTACATAGACTGCTTGGTTTTTCCACCAAGTGACGGCTCCGGCCCAATTTTCCGAGCCGTCAGAAACGGACGCTTGAAGGACAAGACACCTCCCGGTGAGCCAAGGATCGATGAGAAATCTTATGCAGGCCCTTCAGAAGTCGGTCCAGATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

104

Amino Acids

11.22

Weight (kDa)

4.85

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7148 PF23650 18 - 88 1.8e-22 Domain of unknown function (DUF7148)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36145
fragaria_vesca FvH4_6g19360
malus_domestica MD12G1029300.v1.1 MD12G1029500.v1.1 MD14G1029600.v1.1 MD14G1029800.v1.1
prunus_persica Prupe.7G102300_v2.0.a1
pyrus_communis pycom14g02750
rosa_chinensis RchiOBHm_Chr3g0474621
rosa_laevigata RLG00000023907
rosa_multiflora Rmu_sc0001353.1_g000039 Rmu_sc0001353.1_g000048
rosa_roxburghii Rroxscaffold_6G00407040
rosa_rugosa Rorug03G0142200
rosa_samantha Rh3AG192500 Rh3BG222400 Rh3CG217100 Rh3DG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 89
AciI CCGC 2 cut(s) 86, 137
AclWI GGATC 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 273
AdeI CACNNNGTG 1 cut(s) 185
AfaI GTAC 2 cut(s) 68, 158
AfiI CCNNNNNNNGG 2 cut(s) 47, 188
AgsI TTSAA 1 cut(s) 232
Alw26I GTCTC 1 cut(s) 31
AlwI GGATC 1 cut(s) 269
AoxI GGCC 2 cut(s) 196, 283
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
Asp700I GAANNNNTTC 1 cut(s) 59
AspS9I GGNCC 4 cut(s) 148, 197, 284, 299
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 1 cut(s) 263
AvaII GGWCC 2 cut(s) 148, 299
BccI CCATC 1 cut(s) 70
BceAI ACGGC 2 cut(s) 197, 205
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 31
BfaI CTAG 3 cut(s) 42, 108, 310
BfuAI ACCTGC 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 2 cut(s) 148, 299
BmgT120I GGNCC 4 cut(s) 148, 197, 284, 299
BmiI GGNNCC 2 cut(s) 150, 193
BmrFI CCNGG 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 257
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 188
Bse3DI GCAATG 2 cut(s) 52, 69
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 257
BseLI CCNNNNNNNGG 2 cut(s) 47, 188
BseMI GCAATG 2 cut(s) 52, 69
BshFI GGCC 2 cut(s) 198, 285
BshVI ATCGAT 1 cut(s) 264
BsiSI CCGG 2 cut(s) 195, 249
BslFI GGGAC 2 cut(s) 118, 134
BslI CCNNNNNNNGG 2 cut(s) 47, 188
BsmAI GTCTC 1 cut(s) 31
BsmFI GGGAC 2 cut(s) 118, 134
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 2 cut(s) 198, 285
Bso31I GGTCTC 1 cut(s) 31
Bsp1407I TGTACA 2 cut(s) 66, 156
Bsp143I GATC 2 cut(s) 261, 304
BspACI CCGC 2 cut(s) 86, 137
BspANI GGCC 2 cut(s) 198, 285
BspDI ATCGAT 1 cut(s) 264
BspLI GGNNCC 2 cut(s) 150, 193
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 2 cut(s) 52, 69
BsrGI TGTACA 2 cut(s) 66, 156
BssECI CCNNGG 1 cut(s) 257
BssMI GATC 2 cut(s) 261, 304
BssT1I CCWWGG 1 cut(s) 257
BstAUI TGTACA 2 cut(s) 66, 156
BstC8I GCNNGC 1 cut(s) 283
BstKTI GATC 2 cut(s) 264, 307
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 2 cut(s) 261, 304
BstSCI CCNGG 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 2 cut(s) 198, 285
BsuTUI ATCGAT 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 89
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 1 cut(s) 283
Cfr13I GGNCC 4 cut(s) 148, 197, 284, 299
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 234
Csp6I GTAC 2 cut(s) 67, 157
CviJI RGCY 6 cut(s) 32, 192, 198, 212, 256, 285
CviKI_1 RGCY 6 cut(s) 32, 192, 198, 212, 256, 285
CviQI GTAC 2 cut(s) 67, 157
DpnI GATC 2 cut(s) 263, 306
DpnII GATC 2 cut(s) 261, 304
DraIII CACNNNGTG 1 cut(s) 185
EciI GGCGGA 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 2 cut(s) 148, 299
Eco57I CTGAAG 1 cut(s) 273
EcoO109I RGGNCCY 2 cut(s) 148, 284
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
FaiI YATR 4 cut(s) 18, 23, 161, 279
FaqI GGGAC 2 cut(s) 118, 134
FspBI CTAG 3 cut(s) 42, 108, 310
HaeIII GGCC 2 cut(s) 198, 285
HapII CCGG 2 cut(s) 195, 249
HgaI GACGC 1 cut(s) 234
HpaII CCGG 2 cut(s) 195, 249
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 3 cut(s) 209, 218, 292
Hpy188III TCNNGA 1 cut(s) 302
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 226, 297
HpyCH4V TGCA 2 cut(s) 98, 281
Kzo9I GATC 2 cut(s) 261, 304
LmnI GCTCC 1 cut(s) 197
LpnPI CCDG 6 cut(s) 68, 84, 165, 208, 262, 267
MaeI CTAG 3 cut(s) 42, 108, 310
MaeIII GTNAC 2 cut(s) 121, 185
MalI GATC 2 cut(s) 263, 306
MboI GATC 2 cut(s) 261, 304
MfeI CAATTG 1 cut(s) 126
MluCI AATT 3 cut(s) 93, 126, 201
MnlI CCTC 3 cut(s) 133, 139, 255
MroXI GAANNNNTTC 1 cut(s) 59
MslI CAYNNNNRTG 1 cut(s) 74
MspI CCGG 2 cut(s) 195, 249
MspR9I CCNGG 1 cut(s) 249
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 249
NdeII GATC 2 cut(s) 261, 304
NlaIV GGNNCC 2 cut(s) 150, 193
NmuCI GTSAC 1 cut(s) 185
PaqCI CACCTGC 1 cut(s) 89
PctI GAATGC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 148
Psp5II RGGWCCY 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 150, 193
PspPI GGNCC 4 cut(s) 148, 197, 284, 299
PspPPI RGGWCCY 1 cut(s) 148
RsaI GTAC 2 cut(s) 68, 158
RsaNI GTAC 2 cut(s) 67, 157
RseI CAYNNNNRTG 1 cut(s) 74
Sau3AI GATC 2 cut(s) 261, 304
Sau96I GGNCC 4 cut(s) 148, 197, 284, 299
ScrFI CCNGG 1 cut(s) 249
SetI ASST 5 cut(s) 43, 103, 144, 150, 247
SinI GGWCC 2 cut(s) 148, 299
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 3 cut(s) 93, 126, 201
SsiI CCGC 2 cut(s) 86, 137
SspI AATATT 1 cut(s) 58
SspMI CTAG 3 cut(s) 42, 108, 310
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 1 cut(s) 257
TaqI TCGA 1 cut(s) 264
TaqII GACCGA 1 cut(s) 287
TasI AATT 3 cut(s) 93, 126, 201
TatI WGTACW 2 cut(s) 66, 156
TseFI GTSAC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 185
TspGWI ACGGA 1 cut(s) 237
VpaK11BI GGWCC 2 cut(s) 148, 299
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 3 cut(s) 42, 108, 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.