Rh3DG218000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0013540
Physical Location & Seq
Reverse (-)
20257844 .. 20258943
1100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG218000.1

Sequence Viewer

Length: 594 bp
ATGGCCACCACTGCTGTGAATATAAAGCTACTCCACTTCCACTCCCCACGTGCCAATCCCCACGCGCCATCGTTCTCTCCACTTCTCAACAACACCGCCACCACCACCAGCCATGCCTTCCCTAACAACCTCAGTTTACATTCCGCCCACCACTCCCACCCAAATCCAACGGCGGTGGCCAAAGCGGCGCTGGATAAGGACAGCCTTGTGCCCTCCGACGACGCCGAAGATGGAGTCTTGCTTGGTACCATGAAATTACCTGATGATACCGACTTTCCCAGATTCCAAATCTTGCTCTTCCAGTGGGCTAACAGTCTCTGCCAAGGAGCAAATCTACCGCTTGATATGCCTTTGAAGGTTGACAAAATAGCAGGTGGGACTAGATTGGGTTTCATTACAATTGGGGATGGGGAGACGGAGGTCATTGGCTACATTGATTGCTTGGTTTTCCCTCCAGAAGGTGGTTCCGGTCCCATTTTTCGAGCTGTTAGAAATGGACGTTTGAAGGATCGGGTCCTTCCCGGGGAGCCCAGGATCATGAGAAATCTTATGCAGGCTCTTCAGAAGTCGGTTCAAATCGCTAGAGTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.38

Weight (kDa)

7.1

Isoelectric Point (pI)

26.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7148 PF23650 76 - 195 2.9e-57 Domain of unknown function (DUF7148)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36145
fragaria_vesca FvH4_6g19360
malus_domestica MD12G1029300.v1.1 MD12G1029500.v1.1 MD14G1029600.v1.1 MD14G1029800.v1.1
prunus_persica Prupe.7G102300_v2.0.a1
pyrus_communis pycom14g02750
rosa_chinensis RchiOBHm_Chr3g0474621
rosa_laevigata RLG00000023907
rosa_multiflora Rmu_sc0001353.1_g000039 Rmu_sc0001353.1_g000048
rosa_roxburghii Rroxscaffold_6G00407040
rosa_rugosa Rorug03G0142200
rosa_samantha Rh3AG192500 Rh3BG222400 Rh3CG217100 Rh3DG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 362
Acc36I ACCTGC 1 cut(s) 362
Acc65I GGTACC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 461
AccII CGCG 1 cut(s) 65
AciI CCGC 5 cut(s) 96, 144, 173, 185, 338
AclWI GGATC 2 cut(s) 516, 542
AcoI YGGCCR 2 cut(s) 3, 177
AcuI CTGAAG 1 cut(s) 545
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 2 cut(s) 247, 590
AfiI CCNNNNNNNGG 3 cut(s) 458, 461, 523
AgsI TTSAA 3 cut(s) 355, 505, 575
AjnI CCWGG 1 cut(s) 530
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 2 cut(s) 28, 485
AluI AGCT 2 cut(s) 28, 485
Alw26I GTCTC 2 cut(s) 320, 407
AlwI GGATC 2 cut(s) 516, 542
AlwNI CAGNNNCTG 1 cut(s) 318
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 2 cut(s) 3, 177
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
Asp718I GGTACC 1 cut(s) 245
AspLEI GCGC 2 cut(s) 67, 190
AspS9I GGNCC 2 cut(s) 470, 514
AsuC2I CCSGG 2 cut(s) 522, 523
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 2 cut(s) 470, 514
BaeGI GKGCMC 1 cut(s) 213
BalI TGGCCA 2 cut(s) 5, 179
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 531
BbrPI CACGTG 1 cut(s) 50
BccI CCATC 3 cut(s) 76, 224, 401
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 532
BcnI CCSGG 2 cut(s) 522, 523
BcoDI GTCTC 2 cut(s) 320, 407
BfaI CTAG 3 cut(s) 381, 582, 592
BfoI RGCGCY 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 362
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 3 cut(s) 522, 523, 532
Bme18I GGWCC 2 cut(s) 470, 514
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 2 cut(s) 470, 514
BmiI GGNNCC 5 cut(s) 247, 466, 472, 515, 528
BmrFI CCNGG 3 cut(s) 522, 523, 532
BoxI GACNNNNGTC 1 cut(s) 419
BpmI CTGGAG 1 cut(s) 438
BpuMI CCSGG 2 cut(s) 522, 523
BsaAI YACGTR 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 4 cut(s) 322, 521, 522, 530
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 458, 461, 523
Bse1I ACTGG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 532
BseDI CCNNGG 4 cut(s) 322, 521, 522, 530
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 3 cut(s) 458, 461, 523
BseMII CTCAG 1 cut(s) 145
BseNI ACTGG 1 cut(s) 301
BseSI GKGCMC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 2 cut(s) 5, 179
BshNI GGYRCC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 2 cut(s) 468, 522
BslFI GGGAC 2 cut(s) 391, 456
BslI CCNNNNNNNGG 3 cut(s) 458, 461, 523
BsmAI GTCTC 2 cut(s) 320, 407
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 391, 456
BsnI GGCC 2 cut(s) 5, 179
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 213, 531
Bsp143I GATC 2 cut(s) 508, 534
BspACI CCGC 5 cut(s) 96, 144, 173, 185, 338
BspANI GGCC 2 cut(s) 5, 179
BspCNI CTCAG 1 cut(s) 144
BspFNI CGCG 1 cut(s) 65
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 5 cut(s) 247, 466, 472, 515, 528
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 2 cut(s) 516, 542
BspQI GCTCTTC 2 cut(s) 302, 564
BspT107I GGYRCC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 4 cut(s) 322, 521, 522, 530
BssMI GATC 2 cut(s) 508, 534
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 532
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 2 cut(s) 302, 564
BstACI GRCGYC 1 cut(s) 222
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 1 cut(s) 131
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 412
BstFNI CGCG 1 cut(s) 65
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 2 cut(s) 67, 190
BstKTI GATC 2 cut(s) 511, 537
BstMAI GTCTC 2 cut(s) 320, 407
BstMBI GATC 2 cut(s) 508, 534
BstMWI GCNNNNNNNGC 3 cut(s) 11, 185, 346
BstNI CCWGG 1 cut(s) 532
BstPAI GACNNNNGTC 1 cut(s) 419
BstSCI CCNGG 3 cut(s) 520, 521, 530
BstSLI GKGCMC 1 cut(s) 213
BstUI CGCG 1 cut(s) 65
BsuRI GGCC 2 cut(s) 5, 179
BtsCI GGATG 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 2 cut(s) 9, 308
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 318
CciI TCATGA 1 cut(s) 537
CfoI GCGC 2 cut(s) 67, 190
Cfr13I GGNCC 2 cut(s) 470, 514
Cfr9I CCCGGG 1 cut(s) 521
CseI GACGC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 246, 589
CspCI CAANNNNNGTGG 2 cut(s) 156, 191
CviAII CATG 3 cut(s) 113, 250, 538
CviQI GTAC 2 cut(s) 246, 589
DdeI CTNAG 1 cut(s) 131
DpnI GATC 2 cut(s) 510, 536
DpnII GATC 2 cut(s) 508, 534
EaeI YGGCCR 2 cut(s) 3, 177
Eam1104I CTCTTC 2 cut(s) 302, 564
EarI CTCTTC 2 cut(s) 302, 564
EciI GGCGGA 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 322
Eco24I GRGCYC 1 cut(s) 531
Eco47I GGWCC 2 cut(s) 470, 514
Eco57I CTGAAG 1 cut(s) 545
Eco72I CACGTG 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 521
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 530
EcoT14I CCWWGG 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 322
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 3 cut(s) 116, 253, 541
FaiI YATR 6 cut(s) 23, 114, 251, 347, 539, 551
FaqI GGGAC 2 cut(s) 391, 456
FatI CATG 3 cut(s) 112, 249, 537
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 1 cut(s) 419
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 3 cut(s) 381, 582, 592
GlaI GCGC 2 cut(s) 66, 189
GluI GCNGC 1 cut(s) 186
GsuI CTGGAG 1 cut(s) 438
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 2 cut(s) 5, 179
HapII CCGG 2 cut(s) 468, 522
HgaI GACGC 1 cut(s) 230
HhaI GCGC 2 cut(s) 67, 190
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 116, 253, 541
Hin6I GCGC 2 cut(s) 65, 188
HinP1I GCGC 2 cut(s) 65, 188
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 2 cut(s) 234, 282
HpaII CCGG 2 cut(s) 468, 522
Hpy166II GTNNAC 3 cut(s) 137, 361, 589
Hpy188I TCNGA 2 cut(s) 217, 564
Hpy188III TCNNGA 2 cut(s) 455, 538
Hpy8I GTNNAC 3 cut(s) 137, 361, 589
Hpy99I CGWCG 2 cut(s) 221, 224
HpyAV CCTTC 5 cut(s) 127, 349, 452, 499, 527
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 2 cut(s) 49, 499
HpyCH4V TGCA 1 cut(s) 553
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 185, 346
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 2 cut(s) 49, 499
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 116, 253, 541
HspAI GCGC 2 cut(s) 65, 188
KpnI GGTACC 1 cut(s) 249
Kzo9I GATC 2 cut(s) 508, 534
LguI GCTCTTC 2 cut(s) 302, 564
LmnI GCTCC 2 cut(s) 326, 526
MaeI CTAG 3 cut(s) 381, 582, 592
MaeII ACGT 2 cut(s) 49, 499
MalI GATC 2 cut(s) 510, 536
MboI GATC 2 cut(s) 508, 534
MboII GAAGA 3 cut(s) 239, 289, 551
MfeI CAATTG 1 cut(s) 399
MhlI GDGCHC 2 cut(s) 213, 531
MlsI TGGCCA 2 cut(s) 5, 179
MluCI AATT 2 cut(s) 254, 399
MluNI TGGCCA 2 cut(s) 5, 179
MlyI GAGTC 1 cut(s) 243
MmeI TCCRAC 2 cut(s) 191, 240
MnlI CCTC 4 cut(s) 140, 223, 412, 462
Mox20I TGGCCA 2 cut(s) 5, 179
MscI TGGCCA 2 cut(s) 5, 179
MslI CAYNNNNRTG 1 cut(s) 14
Msp20I TGGCCA 2 cut(s) 5, 179
MspI CCGG 2 cut(s) 468, 522
MspR9I CCNGG 3 cut(s) 522, 523, 532
MunI CAATTG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 532
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 3 cut(s) 11, 185, 346
NciI CCSGG 2 cut(s) 522, 523
NdeII GATC 2 cut(s) 508, 534
NlaIII CATG 3 cut(s) 116, 253, 541
NlaIV GGNNCC 5 cut(s) 247, 466, 472, 515, 528
OliI CACNNNNGTG 1 cut(s) 14
PagI TCATGA 1 cut(s) 537
PaqCI CACCTGC 1 cut(s) 362
PciSI GCTCTTC 2 cut(s) 302, 564
PfeI GAWTC 1 cut(s) 282
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 1 cut(s) 187
PleI GAGTC 1 cut(s) 242
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 1 cut(s) 242
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 514
PshAI GACNNNNGTC 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 514
Psp6I CCWGG 1 cut(s) 530
PspCI CACGTG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 530
PspN4I GGNNCC 5 cut(s) 247, 466, 472, 515, 528
PspPI GGNCC 2 cut(s) 470, 514
PspPPI RGGWCCY 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 318
RsaI GTAC 2 cut(s) 247, 590
RsaNI GTAC 2 cut(s) 246, 589
RseI CAYNNNNRTG 1 cut(s) 14
SapI GCTCTTC 2 cut(s) 302, 564
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 508, 534
Sau96I GGNCC 2 cut(s) 470, 514
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 3 cut(s) 522, 523, 532
SduI GDGCHC 2 cut(s) 213, 531
SinI GGWCC 2 cut(s) 470, 514
SmaI CCCGGG 1 cut(s) 523
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 2 cut(s) 254, 399
SsiI CCGC 5 cut(s) 96, 144, 173, 185, 338
SspMI CTAG 3 cut(s) 381, 582, 592
StyD4I CCNGG 3 cut(s) 520, 521, 530
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 2 cut(s) 52, 502
TaqI TCGA 1 cut(s) 481
TasI AATT 2 cut(s) 254, 399
TatI WGTACW 1 cut(s) 588
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 1 cut(s) 282
TscAI CASTG 2 cut(s) 16, 308
TspDTI ATGAA 2 cut(s) 266, 382
TspGWI ACGGA 1 cut(s) 431
TspMI CCCGGG 1 cut(s) 521
TspRI CASTG 2 cut(s) 16, 308
Van91I CCANNNNNTGG 1 cut(s) 461
VpaK11BI GGWCC 2 cut(s) 470, 514
XagI CCTNNNNNAGG 1 cut(s) 456
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XmaI CCCGGG 1 cut(s) 521
XspI CTAG 3 cut(s) 381, 582, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.