MD12G1029500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013540
Physical Location & Seq
Reverse (-)
3223571 .. 3224095
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1029500.v1.1.491

Sequence Viewer

Length: 525 bp
ATGATTAGAAGCTTGGTGAGGCGCTTCTCGCCAACCATTCCCGTCTCGTTAAAGAAGATGAAAGCAGCATATCAGCATATCCTCTACACTTTCGATTCCTCCAACGAATCCCTTTCCCTCTTTCCCATCAATTCTAAAACCCCCTCACAGATTTCATTTGCTCAGCCACCCATGCCCATTTCTGTCGCTGTGGCGGGAGGAGTGATGAGCTCGCTCCGCCGCGTCCACTCCCCACGTGTCAGTTCCCACGTGCTACTCTCCCCTCCTCCTCCGTCGCTGGCTGCTTCCTGTCCACTAGTATCCACCACCGCCACCAGCTGCTGCTTCTTTCCTAACCTTAATTTGCATTCCGGCCGTCACTACCGCCACCGAAATCAAACGGCGGTGGCCAAGGCGTCGTCTCAGGAAAAGGACAGCCTTATCCCTTCGGACGACGCCGAAGACGGCGTCTTGCTCGGGACCATGAAATTGCCCTCGGATACCGATCTTCCCAGATTCCAAATTCTACTATTCCAGGTAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.0

Weight (kDa)

9.72

Isoelectric Point (pI)

73.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7148 PF23650 147 - 173 6.2e-07 Domain of unknown function (DUF7148)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36145
fragaria_vesca FvH4_6g19360
malus_domestica MD12G1029300.v1.1 MD12G1029500.v1.1 MD14G1029600.v1.1 MD14G1029800.v1.1
prunus_persica Prupe.7G102300_v2.0.a1
pyrus_communis pycom14g02750
rosa_chinensis RchiOBHm_Chr3g0474621
rosa_laevigata RLG00000023907
rosa_multiflora Rmu_sc0001353.1_g000039 Rmu_sc0001353.1_g000048
rosa_roxburghii Rroxscaffold_6G00407040
rosa_rugosa Rorug03G0142200
rosa_samantha Rh3AG192500 Rh3BG222400 Rh3CG217100 Rh3DG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 222
AciI CCGC 6 cut(s) 194, 217, 220, 309, 364, 383
AcoI YGGCCR 2 cut(s) 352, 387
AcsI RAATTY 1 cut(s) 501
AcvI CACGTG 2 cut(s) 236, 250
AcyI GRCGYC 3 cut(s) 395, 435, 447
AflIII ACRYGT 1 cut(s) 235
AhlI ACTAGT 1 cut(s) 295
AjnI CCWGG 1 cut(s) 513
AluBI AGCT 3 cut(s) 12, 210, 318
AluI AGCT 3 cut(s) 12, 210, 318
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 2 cut(s) 49, 405
AlwNI CAGNNNCTG 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 2 cut(s) 352, 387
ApeKI GCWGC 4 cut(s) 65, 281, 318, 321
ApoI RAATTY 1 cut(s) 501
ArsI GACNNNNNNTTYG 2 cut(s) 432, 464
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 459
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 1 cut(s) 459
BalI TGGCCA 1 cut(s) 389
BanII GRGCYC 1 cut(s) 212
BbrPI CACGTG 2 cut(s) 236, 250
BbsI GAAGAC 1 cut(s) 447
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 4 cut(s) 77, 268, 305, 308
BccI CCATC 1 cut(s) 134
BceAI ACGGC 3 cut(s) 339, 396, 460
BciT130I CCWGG 1 cut(s) 515
BciVI GTATCC 2 cut(s) 310, 472
BcoDI GTCTC 2 cut(s) 49, 405
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 25
BfuI GTATCC 2 cut(s) 310, 472
BisI GCNGC 5 cut(s) 66, 220, 282, 319, 322
BlpI GCTNAGC 1 cut(s) 162
BlsI GCNGC 5 cut(s) 67, 221, 283, 320, 323
Bme1390I CCNGG 1 cut(s) 515
Bme18I GGWCC 1 cut(s) 459
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 459
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 1 cut(s) 515
BpiI GAAGAC 1 cut(s) 447
Bpu1102I GCTNAGC 1 cut(s) 162
BsaAI YACGTR 2 cut(s) 236, 250
BsaBI GATNNNNATC 1 cut(s) 483
BsaHI GRCGYC 3 cut(s) 395, 435, 447
BsaJI CCNNGG 2 cut(s) 390, 474
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 2 cut(s) 390, 474
BseJI GATNNNNATC 1 cut(s) 483
BseMII CTCAG 2 cut(s) 176, 416
BseRI GAGGAG 3 cut(s) 213, 255, 258
BseX3I CGGCCG 1 cut(s) 352
BseXI GCAGC 4 cut(s) 77, 268, 305, 308
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 2 cut(s) 354, 389
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 1 cut(s) 472
BsmAI GTCTC 2 cut(s) 49, 405
BsmBI CGTCTC 2 cut(s) 49, 405
BsmFI GGGAC 1 cut(s) 472
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 2 cut(s) 354, 389
BsoBI CYCGRG 1 cut(s) 455
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 1 cut(s) 484
Bsp1720I GCTNAGC 1 cut(s) 162
BspACI CCGC 6 cut(s) 194, 217, 220, 309, 364, 383
BspANI GGCC 2 cut(s) 354, 389
BspCNI CTCAG 2 cut(s) 175, 415
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 390, 474
BssMI GATC 1 cut(s) 484
BssNI GRCGYC 3 cut(s) 395, 435, 447
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 515
BstACI GRCGYC 3 cut(s) 395, 435, 447
BstBAI YACGTR 2 cut(s) 236, 250
BstC8I GCNNGC 2 cut(s) 212, 279
BstDEI CTNAG 2 cut(s) 162, 402
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 25
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 1 cut(s) 487
BstMAI GTCTC 2 cut(s) 49, 405
BstMBI GATC 1 cut(s) 484
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 3 cut(s) 28, 172, 216
BstNI CCWGG 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 513
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 77, 268, 305, 308
BstV2I GAAGAC 1 cut(s) 447
BstZI CGGCCG 1 cut(s) 352
BsuI GTATCC 2 cut(s) 310, 472
BsuRI GGCC 2 cut(s) 354, 389
Cac8I GCNNGC 2 cut(s) 212, 279
CaiI CAGNNNCTG 1 cut(s) 321
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 459
CseI GACGC 4 cut(s) 211, 384, 436, 443
CviAII CATG 2 cut(s) 172, 463
CviJI RGCY 8 cut(s) 12, 166, 210, 281, 318, 354, 389, 417
CviKI_1 RGCY 8 cut(s) 12, 166, 210, 281, 318, 354, 389, 417
DdeI CTNAG 2 cut(s) 162, 402
DpnI GATC 1 cut(s) 486
DpnII GATC 1 cut(s) 484
EaeI YGGCCR 2 cut(s) 352, 387
EagI CGGCCG 1 cut(s) 352
EciI GGCGGA 1 cut(s) 206
Ecl136II GAGCTC 1 cut(s) 210
EclXI CGGCCG 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 459
Eco52I CGGCCG 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 210
Eco72I CACGTG 2 cut(s) 236, 250
Eco88I CYCGRG 1 cut(s) 455
EcoICRI GAGCTC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 513
EcoT14I CCWWGG 1 cut(s) 390
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 1 cut(s) 390
Esp3I CGTCTC 2 cut(s) 49, 405
FaeI CATG 2 cut(s) 175, 466
FaiI YATR 5 cut(s) 70, 78, 173, 464, 523
FaqI GGGAC 1 cut(s) 472
FatI CATG 2 cut(s) 171, 462
FauI CCCGC 1 cut(s) 187
Fnu4HI GCNGC 5 cut(s) 66, 220, 282, 319, 322
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 5 cut(s) 66, 220, 282, 319, 322
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 23
GluI GCNGC 5 cut(s) 66, 220, 282, 319, 322
HaeII RGCGCY 1 cut(s) 25
HaeIII GGCC 2 cut(s) 354, 389
HapII CCGG 1 cut(s) 351
HgaI GACGC 4 cut(s) 211, 384, 436, 443
HhaI GCGC 1 cut(s) 24
Hin1I GRCGYC 3 cut(s) 395, 435, 447
Hin1II CATG 2 cut(s) 175, 466
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 3 cut(s) 95, 107, 495
HpaII CCGG 1 cut(s) 351
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 226, 293
Hpy188I TCNGA 2 cut(s) 430, 478
Hpy188III TCNNGA 2 cut(s) 404, 457
Hpy8I GTNNAC 2 cut(s) 226, 293
Hpy99I CGWCG 3 cut(s) 277, 400, 437
HpyAV CCTTC 1 cut(s) 435
HpyCH4IV ACGT 2 cut(s) 235, 249
HpyCH4V TGCA 1 cut(s) 346
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 172, 216
HpyF3I CTNAG 2 cut(s) 162, 402
HpySE526I ACGT 2 cut(s) 235, 249
Hsp92I GRCGYC 3 cut(s) 395, 435, 447
Hsp92II CATG 2 cut(s) 175, 466
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 1 cut(s) 484
LmnI GCTCC 1 cut(s) 219
LpnPI CCDG 7 cut(s) 263, 301, 328, 364, 389, 500, 505
Lsp1109I GCAGC 4 cut(s) 77, 268, 305, 308
MaeI CTAG 1 cut(s) 296
MaeII ACGT 2 cut(s) 235, 249
MaeIII GTNAC 1 cut(s) 356
MalI GATC 1 cut(s) 486
MboI GATC 1 cut(s) 484
MboII GAAGA 3 cut(s) 67, 452, 479
MhlI GDGCHC 1 cut(s) 212
MlsI TGGCCA 1 cut(s) 389
MluCI AATT 4 cut(s) 130, 340, 467, 501
MluNI TGGCCA 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 126
Mox20I TGGCCA 1 cut(s) 389
MscI TGGCCA 1 cut(s) 389
MseI TTAA 2 cut(s) 50, 339
Msp20I TGGCCA 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 28, 172, 216
NdeII GATC 1 cut(s) 484
NlaIII CATG 2 cut(s) 175, 466
NlaIV GGNNCC 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 356
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 3 cut(s) 95, 107, 495
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 5 cut(s) 67, 221, 283, 320, 323
PmaCI CACGTG 2 cut(s) 236, 250
PmlI CACGTG 2 cut(s) 236, 250
Ppu21I YACGTR 2 cut(s) 236, 250
Psp124BI GAGCTC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 513
PspCI CACGTG 2 cut(s) 236, 250
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 1 cut(s) 459
PstNI CAGNNNCTG 1 cut(s) 321
PsyI GACNNNGTC 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 318
SacI GAGCTC 1 cut(s) 212
SaqAI TTAA 2 cut(s) 50, 339
SatI GCNGC 5 cut(s) 66, 220, 282, 319, 322
Sau3AI GATC 1 cut(s) 484
Sau96I GGNCC 1 cut(s) 459
ScrFI CCNGG 1 cut(s) 515
SduI GDGCHC 1 cut(s) 212
SetI ASST 7 cut(s) 14, 212, 238, 252, 320, 339, 519
SinI GGWCC 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 4 cut(s) 130, 340, 467, 501
SsiI CCGC 6 cut(s) 194, 217, 220, 309, 364, 383
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 513
StyI CCWWGG 1 cut(s) 390
TaiI ACGT 2 cut(s) 238, 252
TaqI TCGA 1 cut(s) 93
TasI AATT 4 cut(s) 130, 340, 467, 501
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 3 cut(s) 95, 107, 495
Tru1I TTAA 2 cut(s) 50, 339
Tru9I TTAA 2 cut(s) 50, 339
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 4 cut(s) 65, 281, 318, 321
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 3 cut(s) 74, 144, 479
TspGWI ACGGA 1 cut(s) 261
Tth111I GACNNNGTC 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 459
XapI RAATTY 1 cut(s) 501
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.