RLG00000023907

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013540
Physical Location & Seq
Forward (+)
29841431 .. 29842484
1054 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023907

Sequence Viewer

Length: 591 bp
ATGGCCGCCACTGCTGTGAATATGAAGCTACTCCACTTTCACTCCCCACGTGCCAATCCCCACCCGCCATCGTTCTCCCCACTTCTCACCAACACCACCACCACCAGCCATGCCTTCCCTAACAACCTCAGTTTACGTTCCGGCCACTCCCACCACCCAAATCCAACGGCGGTGGTCAAAGCGGCGCTGGATAAGGACAGCCTTGTGCCCTCCGACGACGCCGAAGATGGAGTCTTGCTTGGTACCATGAAATTGCCTGATGATACTGACTTTCCCAGATTCCAAATCTTGCTCTTCCAGTGGGCTAACAGTCTCTGCCAAGGAGCAAATCTACCGCTTGATATGCCTTTGAAGGTTGACAAAATAGCAGGTGGGACTAGATTGGGTTTCATTACAATTGGGGATGGGGAGACAGAGGTCATTGGCTACATTGATTGCTTGGTTTTCCCTCCACAAGGTGGTTCCGGTCCCATTTTTCGAGCTGTTAGAAATGGACGTTTGAAGGATCGGGTCCTTCCCGGGGAGCCCAGGATCATGAGAAATCTTATGCAGGCTCTTCAGAAGTCGGTTCAAATCGCTAGGGTGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.34

Weight (kDa)

8.64

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7148 PF23650 75 - 194 2.8e-57 Domain of unknown function (DUF7148)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36145
fragaria_vesca FvH4_6g19360
malus_domestica MD12G1029300.v1.1 MD12G1029500.v1.1 MD14G1029600.v1.1 MD14G1029800.v1.1
prunus_persica Prupe.7G102300_v2.0.a1
pyrus_communis pycom14g02750
rosa_chinensis RchiOBHm_Chr3g0474621
rosa_laevigata RLG00000023907
rosa_multiflora Rmu_sc0001353.1_g000039 Rmu_sc0001353.1_g000048
rosa_roxburghii Rroxscaffold_6G00407040
rosa_rugosa Rorug03G0142200
rosa_samantha Rh3AG192500 Rh3BG222400 Rh3CG217100 Rh3DG218000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 359
Acc36I ACCTGC 1 cut(s) 359
Acc65I GGTACC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 458
AciI CCGC 5 cut(s) 6, 65, 170, 182, 335
AclWI GGATC 2 cut(s) 513, 539
AcoI YGGCCR 2 cut(s) 3, 142
AcuI CTGAAG 1 cut(s) 542
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 458
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 3 cut(s) 455, 458, 520
AgsI TTSAA 3 cut(s) 352, 502, 572
AjnI CCWGG 1 cut(s) 527
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 2 cut(s) 28, 482
AluI AGCT 2 cut(s) 28, 482
Alw26I GTCTC 2 cut(s) 317, 404
AlwI GGATC 2 cut(s) 513, 539
AlwNI CAGNNNCTG 1 cut(s) 315
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 2 cut(s) 3, 142
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
Asp718I GGTACC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 2 cut(s) 467, 511
AsuC2I CCSGG 2 cut(s) 519, 520
AsuHPI GGTGA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 2 cut(s) 467, 511
BaeGI GKGCMC 1 cut(s) 210
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 528
BbrPI CACGTG 1 cut(s) 50
BccI CCATC 3 cut(s) 76, 221, 398
BceAI ACGGC 1 cut(s) 183
BciT130I CCWGG 1 cut(s) 529
BcnI CCSGG 2 cut(s) 519, 520
BcoDI GTCTC 2 cut(s) 317, 404
BfaI CTAG 2 cut(s) 378, 579
BfoI RGCGCY 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 359
BisI GCNGC 2 cut(s) 6, 183
BlsI GCNGC 2 cut(s) 7, 184
Bme1390I CCNGG 3 cut(s) 519, 520, 529
Bme18I GGWCC 2 cut(s) 467, 511
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 467, 511
BmiI GGNNCC 5 cut(s) 244, 463, 469, 512, 525
BmrFI CCNGG 3 cut(s) 519, 520, 529
BoxI GACNNNNGTC 1 cut(s) 416
BpuMI CCSGG 2 cut(s) 519, 520
BsaAI YACGTR 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 4 cut(s) 319, 518, 519, 527
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 455, 458, 520
Bse1I ACTGG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 529
BseDI CCNNGG 4 cut(s) 319, 518, 519, 527
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 3 cut(s) 455, 458, 520
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 298
BseSI GKGCMC 1 cut(s) 210
BshFI GGCC 2 cut(s) 5, 144
BshNI GGYRCC 1 cut(s) 242
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 3 cut(s) 141, 465, 519
BslFI GGGAC 2 cut(s) 388, 453
BslI CCNNNNNNNGG 3 cut(s) 455, 458, 520
BsmAI GTCTC 2 cut(s) 317, 404
BsmFI GGGAC 2 cut(s) 388, 453
BsnI GGCC 2 cut(s) 5, 144
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 210, 528
Bsp143I GATC 2 cut(s) 505, 531
BspACI CCGC 5 cut(s) 6, 65, 170, 182, 335
BspANI GGCC 2 cut(s) 5, 144
BspCNI CTCAG 1 cut(s) 141
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 5 cut(s) 244, 463, 469, 512, 525
BspMI ACCTGC 1 cut(s) 359
BspPI GGATC 2 cut(s) 513, 539
BspQI GCTCTTC 2 cut(s) 299, 561
BspT107I GGYRCC 1 cut(s) 242
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 4 cut(s) 319, 518, 519, 527
BssMI GATC 2 cut(s) 505, 531
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 319
Bst2UI CCWGG 1 cut(s) 529
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 2 cut(s) 299, 561
BstACI GRCGYC 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 1 cut(s) 128
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 1 cut(s) 409
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 2 cut(s) 508, 534
BstMAI GTCTC 2 cut(s) 317, 404
BstMBI GATC 2 cut(s) 505, 531
BstMWI GCNNNNNNNGC 2 cut(s) 11, 343
BstNI CCWGG 1 cut(s) 529
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 517, 518, 527
BstSLI GKGCMC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 5, 144
BtsCI GGATG 1 cut(s) 409
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 2 cut(s) 9, 305
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 552
CaiI CAGNNNCTG 1 cut(s) 315
CciI TCATGA 1 cut(s) 534
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 467, 511
Cfr9I CCCGGG 1 cut(s) 518
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 243
CspCI CAANNNNNGTGG 2 cut(s) 153, 188
CviAII CATG 3 cut(s) 110, 247, 535
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 1 cut(s) 128
DpnI GATC 2 cut(s) 507, 533
DpnII GATC 2 cut(s) 505, 531
DraIII CACNNNGTG 1 cut(s) 458
EaeI YGGCCR 2 cut(s) 3, 142
Eam1104I CTCTTC 2 cut(s) 299, 561
EarI CTCTTC 2 cut(s) 299, 561
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 467, 511
Eco57I CTGAAG 1 cut(s) 542
Eco72I CACGTG 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 518
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 527
EcoT14I CCWWGG 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 3 cut(s) 113, 250, 538
FaiI YATR 6 cut(s) 23, 111, 248, 344, 536, 548
FaqI GGGAC 2 cut(s) 388, 453
FatI CATG 3 cut(s) 109, 246, 534
FauI CCCGC 1 cut(s) 72
Fnu4HI GCNGC 2 cut(s) 6, 183
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 2 cut(s) 6, 183
FspBI CTAG 2 cut(s) 378, 579
GlaI GCGC 1 cut(s) 186
GluI GCNGC 2 cut(s) 6, 183
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 2 cut(s) 5, 144
HapII CCGG 3 cut(s) 141, 465, 519
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 3 cut(s) 113, 250, 538
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 2 cut(s) 231, 279
HpaII CCGG 3 cut(s) 141, 465, 519
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 134, 358
Hpy188I TCNGA 2 cut(s) 214, 561
Hpy188III TCNNGA 1 cut(s) 535
Hpy8I GTNNAC 2 cut(s) 134, 358
Hpy99I CGWCG 2 cut(s) 218, 221
HpyAV CCTTC 4 cut(s) 124, 346, 496, 524
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 3 cut(s) 49, 136, 496
HpyCH4V TGCA 1 cut(s) 550
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 343
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 3 cut(s) 49, 136, 496
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 3 cut(s) 113, 250, 538
HspAI GCGC 1 cut(s) 185
KpnI GGTACC 1 cut(s) 246
Kzo9I GATC 2 cut(s) 505, 531
LguI GCTCTTC 2 cut(s) 299, 561
LmnI GCTCC 2 cut(s) 323, 523
MaeI CTAG 2 cut(s) 378, 579
MaeII ACGT 3 cut(s) 49, 136, 496
MalI GATC 2 cut(s) 507, 533
MboI GATC 2 cut(s) 505, 531
MboII GAAGA 3 cut(s) 236, 286, 548
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 210, 528
MluCI AATT 2 cut(s) 251, 396
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 2 cut(s) 188, 237
MnlI CCTC 4 cut(s) 137, 220, 409, 459
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 3 cut(s) 141, 465, 519
MspR9I CCNGG 3 cut(s) 519, 520, 529
MunI CAATTG 1 cut(s) 396
MvaI CCWGG 1 cut(s) 529
MwoI GCNNNNNNNGC 2 cut(s) 11, 343
NciI CCSGG 2 cut(s) 519, 520
NdeII GATC 2 cut(s) 505, 531
NlaIII CATG 3 cut(s) 113, 250, 538
NlaIV GGNNCC 5 cut(s) 244, 463, 469, 512, 525
OliI CACNNNNGTG 1 cut(s) 14
PagI TCATGA 1 cut(s) 534
PaqCI CACCTGC 1 cut(s) 359
PciSI GCTCTTC 2 cut(s) 299, 561
PfeI GAWTC 1 cut(s) 279
PflMI CCANNNNNTGG 1 cut(s) 458
PkrI GCNGC 2 cut(s) 7, 184
PleI GAGTC 1 cut(s) 239
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 1 cut(s) 239
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 511
PshAI GACNNNNGTC 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 527
PspCI CACGTG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 527
PspN4I GGNNCC 5 cut(s) 244, 463, 469, 512, 525
PspPI GGNCC 2 cut(s) 467, 511
PspPPI RGGWCCY 1 cut(s) 511
PstNI CAGNNNCTG 1 cut(s) 315
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 1 cut(s) 14
SapI GCTCTTC 2 cut(s) 299, 561
SatI GCNGC 2 cut(s) 6, 183
Sau3AI GATC 2 cut(s) 505, 531
Sau96I GGNCC 2 cut(s) 467, 511
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 3 cut(s) 519, 520, 529
SduI GDGCHC 2 cut(s) 210, 528
SinI GGWCC 2 cut(s) 467, 511
SmaI CCCGGG 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 2 cut(s) 251, 396
SsiI CCGC 5 cut(s) 6, 65, 170, 182, 335
SspMI CTAG 2 cut(s) 378, 579
StyD4I CCNGG 3 cut(s) 517, 518, 527
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 3 cut(s) 52, 139, 499
TaqI TCGA 1 cut(s) 478
TasI AATT 2 cut(s) 251, 396
TauI GCSGC 2 cut(s) 8, 185
TfiI GAWTC 1 cut(s) 279
TscAI CASTG 2 cut(s) 16, 305
TspDTI ATGAA 3 cut(s) 38, 263, 379
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 2 cut(s) 16, 305
Van91I CCANNNNNTGG 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 467, 511
XagI CCTNNNNNAGG 1 cut(s) 453
XmaI CCCGGG 1 cut(s) 518
XspI CTAG 2 cut(s) 378, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.