MD12G1050300.v1.1

Homogentisate phytyltransferase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008712
Physical Location & Seq
Forward (+)
5711620 .. 5715769
4150 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1050300.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGGAGCTTTCTTCTCTGCGGTTCCCAGCTATAACACCTCACTGCAGCACCTCATCCTCTTTGCCTCCAATTTCTGTTCGGCCCACCACTACAAAATCGGTTGCTTTTCTCTCAAGCTGCAGGACAAAGTTGTACTCAATTGGGGTGTCCAGAAGCAGCTCCAAATCCTTGCTTAACTGCCAAAAGAGGCGAAATTCCATCTGGGCCTGCAGTCAAGTTGGAGCTGCTGAATCTGATCCAGTATTGACCAAAGTTTCAGATTTTAAAGATGCTTGTTGGAGATTCTTGAGGCCCCATACCATACGTGGGACAGTTCTGGGGTCTACTGCTTTGGTGGCAAGAGCATTGATTGAGAACTCCAATCTGATAAAGTGGTCTCTGCTGTTTAAGGCATTCTCTGGTCTTTTTGCTCTACTATGTGGGAATGGTTATATAGTTGGCATCAATCAGATTTACGATATCAAAATTGACAAGGTAAACAAACCTTATTTACCTATCGCTGCTGGGGATCTTTCAGTTAAATCAGCATGGCTCTTGGTGGTATTTTTTGCGGTGACTGGCTTGTTGATCGTTGGACTGAACTTTGGGCCATTCATTACTTCTCTCTACTGTCTTGGTCTTTTCCTTGGCACCATATATTCTGTTCCTCCGCTTAGAATGAAAAGATTTCCTGTTGCTGCTTTTCTTATAATTGCCACGGTACGGGGTTTCCTTCTCAATTTCGGTGTATATTATGCCACTAGAGCTGCCCTTGGGCTTCAATTTGAGTGGAGCTCGGCCGTTGCTTTTATCACTACCTTTGTAACATTGTTTGCTTTGGTTATTGCGATAACAAAAGATCTTCCGGATGTAGAGGGGGACCGCAAGTTTGAAATATCAACGTTTGCAACAAAACTTGGAGTTAGAAACATTGCGTTCCTTGGTTCTGGGCTTCTGCTGCTAAATTATGTTGGTTCCATATTGATAGCAAGTTTAATGCCGGAGGCATTCAGACGTAGCTTAATGATACCCATGCATGCAATCTTGGCACTGAGTTTAGTTTTCCAGACTTGGGTGCTCGAGAAAGCGAATTACACCAAGGAAGCAATTGCCAACTACTACAGATTTATTTGGAATCTCTTCTATGCCGAGTACATCCTTTTCCCCTTCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 map00130
Pfam Domains
Protein Families
UbiA

Protein Analysis

385

Amino Acids

42.54

Weight (kDa)

9.72

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 108 - 373 2.3e-37 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AccB1I GGYRCC 1 cut(s) 629
AccI GTMKAC 1 cut(s) 323
AccIII TCCGGA 1 cut(s) 844
AciI CCGC 4 cut(s) 19, 551, 650, 862
AclI AACGTT 1 cut(s) 881
AclWI GGATC 2 cut(s) 230, 516
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 1 cut(s) 193
AfaI GTAC 3 cut(s) 134, 702, 1133
AfiI CCNNNNNNNGG 3 cut(s) 306, 702, 1051
AgsI TTSAA 2 cut(s) 761, 872
AluBI AGCT 8 cut(s) 7, 29, 117, 159, 224, 746, 774, 999
AluI AGCT 8 cut(s) 7, 29, 117, 159, 224, 746, 774, 999
Alw21I GWGCWC 2 cut(s) 776, 1059
Alw26I GTCTC 1 cut(s) 381
AlwI GGATC 2 cut(s) 230, 516
Ama87I CYCGRG 1 cut(s) 1058
Aor13HI TCCGGA 1 cut(s) 844
AoxI GGCC 5 cut(s) 80, 204, 290, 587, 777
ApeKI GCWGC 8 cut(s) 45, 117, 156, 224, 500, 677, 746, 937
ApoI RAATTY 1 cut(s) 193
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
Asp700I GAANNNNTTC 1 cut(s) 1118
AspS9I GGNCC 5 cut(s) 81, 204, 291, 587, 859
AsuHPI GGTGA 1 cut(s) 565
AvaI CYCGRG 1 cut(s) 1058
AvaII GGWCC 1 cut(s) 859
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 776
Bbv12I GWGCWC 2 cut(s) 776, 1059
BbvI GCAGC 8 cut(s) 57, 104, 168, 211, 487, 664, 733, 924
BccI CCATC 1 cut(s) 206
BceAI ACGGC 1 cut(s) 764
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 2 cut(s) 741, 1153
BfmI CTRYAG 4 cut(s) 43, 118, 208, 1099
BglII AGATCT 1 cut(s) 838
BisI GCNGC 8 cut(s) 46, 118, 157, 225, 501, 678, 747, 938
BlsI GCNGC 8 cut(s) 47, 119, 158, 226, 502, 679, 748, 939
Bme18I GGWCC 1 cut(s) 859
BmeT110I CYCGRG 1 cut(s) 1058
BmgT120I GGNCC 5 cut(s) 81, 204, 291, 587, 859
BmiI GGNNCC 5 cut(s) 23, 293, 631, 860, 955
BmsI GCATC 2 cut(s) 259, 450
BplI GAGNNNNNCTC 2 cut(s) 758, 790
BpuEI CTTGAG 2 cut(s) 97, 307
BsaAI YACGTR 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 5 cut(s) 625, 696, 751, 919, 1077
BsaWI WCCGGW 1 cut(s) 844
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 702, 1051
Bse1I ACTGG 2 cut(s) 239, 562
Bse3DI GCAATG 1 cut(s) 909
BseAI TCCGGA 1 cut(s) 844
BseDI CCNNGG 5 cut(s) 625, 696, 751, 919, 1077
BseGI GGATG 3 cut(s) 53, 853, 1134
BseLI CCNNNNNNNGG 3 cut(s) 306, 702, 1051
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 1 cut(s) 1022
BseNI ACTGG 2 cut(s) 239, 562
BseX3I CGGCCG 1 cut(s) 777
BseXI GCAGC 8 cut(s) 57, 104, 168, 211, 487, 664, 733, 924
BseYI CCCAGC 2 cut(s) 25, 503
Bsh1285I CGRYCG 1 cut(s) 780
BshFI GGCC 5 cut(s) 82, 206, 292, 589, 779
BshNI GGYRCC 1 cut(s) 629
BsiEI CGRYCG 1 cut(s) 780
BsiHKAI GWGCWC 2 cut(s) 776, 1059
BsiHKCI CYCGRG 1 cut(s) 1058
BsiSI CCGG 2 cut(s) 845, 980
BslFI GGGAC 2 cut(s) 322, 872
BslI CCNNNNNNNGG 3 cut(s) 306, 702, 1051
BsmAI GTCTC 1 cut(s) 381
BsmFI GGGAC 2 cut(s) 322, 872
BsmI GAATGC 2 cut(s) 392, 986
BsnI GGCC 5 cut(s) 82, 206, 292, 589, 779
Bso31I GGTCTC 1 cut(s) 381
BsoBI CYCGRG 1 cut(s) 1058
Bsp1286I GDGCHC 2 cut(s) 776, 1059
Bsp13I TCCGGA 1 cut(s) 844
Bsp143I GATC 4 cut(s) 235, 508, 567, 838
BspACI CCGC 4 cut(s) 19, 551, 650, 862
BspANI GGCC 5 cut(s) 82, 206, 292, 589, 779
BspCNI CTCAG 1 cut(s) 1023
BspEI TCCGGA 1 cut(s) 844
BspLI GGNNCC 5 cut(s) 23, 293, 631, 860, 955
BspMAI CTGCAG 3 cut(s) 47, 122, 212
BspPI GGATC 2 cut(s) 230, 516
BspT107I GGYRCC 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 381
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 2 cut(s) 239, 562
BssECI CCNNGG 5 cut(s) 625, 696, 751, 919, 1077
BssMI GATC 4 cut(s) 235, 508, 567, 838
BssT1I CCWWGG 4 cut(s) 625, 751, 919, 1077
Bst4CI ACNGT 3 cut(s) 313, 611, 700
Bst6I CTCTTC 1 cut(s) 1124
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 208, 1017
BstDEI CTNAG 2 cut(s) 653, 1031
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 3 cut(s) 53, 853, 1134
BstKTI GATC 4 cut(s) 238, 511, 570, 841
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 4 cut(s) 235, 508, 567, 838
BstMCI CGRYCG 1 cut(s) 780
BstMWI GCNNNNNNNGC 4 cut(s) 335, 743, 937, 1025
BstNSI RCATGY 1 cut(s) 1019
BstSFI CTRYAG 4 cut(s) 43, 118, 208, 1099
BstV1I GCAGC 8 cut(s) 57, 104, 168, 211, 487, 664, 733, 924
BstX2I RGATCY 2 cut(s) 508, 838
BstYI RGATCY 2 cut(s) 508, 838
BstZI CGGCCG 1 cut(s) 777
BsuRI GGCC 5 cut(s) 82, 206, 292, 589, 779
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 3 cut(s) 53, 853, 1134
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 2 cut(s) 40, 1028
Cac8I GCNNGC 2 cut(s) 208, 1017
Cfr13I GGNCC 5 cut(s) 81, 204, 291, 587, 859
Csp6I GTAC 3 cut(s) 133, 701, 1132
CspCI CAANNNNNGTGG 2 cut(s) 749, 784
CviAII CATG 3 cut(s) 528, 1012, 1016
CviQI GTAC 3 cut(s) 133, 701, 1132
DdeI CTNAG 2 cut(s) 653, 1031
DpnI GATC 4 cut(s) 237, 510, 569, 840
DpnII GATC 4 cut(s) 235, 508, 567, 838
DraI TTTAAA 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 777
EagI CGGCCG 1 cut(s) 777
Eam1104I CTCTTC 1 cut(s) 1124
EarI CTCTTC 1 cut(s) 1124
Ecl136II GAGCTC 1 cut(s) 774
EclXI CGGCCG 1 cut(s) 777
Eco130I CCWWGG 4 cut(s) 625, 751, 919, 1077
Eco24I GRGCYC 1 cut(s) 776
Eco31I GGTCTC 1 cut(s) 381
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 859
Eco52I CGGCCG 1 cut(s) 777
Eco53kI GAGCTC 1 cut(s) 774
Eco88I CYCGRG 1 cut(s) 1058
EcoICRI GAGCTC 1 cut(s) 774
EcoO109I RGGNCCY 1 cut(s) 291
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 4 cut(s) 625, 751, 919, 1077
EcoT22I ATGCAT 1 cut(s) 1017
EcoT38I GRGCYC 1 cut(s) 776
ErhI CCWWGG 4 cut(s) 625, 751, 919, 1077
FaeI CATG 3 cut(s) 531, 1015, 1019
FaqI GGGAC 2 cut(s) 322, 872
FatI CATG 3 cut(s) 527, 1011, 1015
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 8 cut(s) 46, 118, 157, 225, 501, 678, 747, 938
FokI GGATG 3 cut(s) 40, 860, 1121
FriOI GRGCYC 1 cut(s) 776
Fsp4HI GCNGC 8 cut(s) 46, 118, 157, 225, 501, 678, 747, 938
FspBI CTAG 2 cut(s) 741, 1153
GluI GCNGC 8 cut(s) 46, 118, 157, 225, 501, 678, 747, 938
GsaI CCCAGC 2 cut(s) 29, 507
HaeIII GGCC 5 cut(s) 82, 206, 292, 589, 779
HapII CCGG 2 cut(s) 845, 980
Hin1II CATG 3 cut(s) 531, 1015, 1019
HinfI GANTC 3 cut(s) 230, 282, 1114
HpaII CCGG 2 cut(s) 845, 980
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 2 cut(s) 324, 478
Hpy188I TCNGA 5 cut(s) 235, 259, 366, 450, 992
Hpy188III TCNNGA 5 cut(s) 150, 286, 845, 1045, 1060
Hpy8I GTNNAC 2 cut(s) 324, 478
HpyAV CCTTC 1 cut(s) 722
HpyCH4III ACNGT 3 cut(s) 313, 611, 700
HpyCH4IV ACGT 3 cut(s) 304, 881, 994
HpyCH4V TGCA 6 cut(s) 45, 120, 210, 887, 1015, 1019
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 743, 937, 1025
HpyF3I CTNAG 2 cut(s) 653, 1031
HpySE526I ACGT 3 cut(s) 304, 881, 994
Hsp92II CATG 3 cut(s) 531, 1015, 1019
Kpn2I TCCGGA 1 cut(s) 844
Kzo9I GATC 4 cut(s) 235, 508, 567, 838
LmnI GCTCC 4 cut(s) 4, 164, 221, 771
Lsp1109I GCAGC 8 cut(s) 57, 104, 168, 211, 487, 664, 733, 924
LweI GCATC 2 cut(s) 259, 450
MaeI CTAG 2 cut(s) 741, 1153
MaeII ACGT 3 cut(s) 304, 881, 994
MaeIII GTNAC 2 cut(s) 553, 802
MalI GATC 4 cut(s) 237, 510, 569, 840
MboI GATC 4 cut(s) 235, 508, 567, 838
MboII GAAGA 3 cut(s) 3, 833, 1111
MfeI CAATTG 2 cut(s) 138, 1086
MflI RGATCY 2 cut(s) 508, 838
MhlI GDGCHC 2 cut(s) 776, 1059
MmeI TCCRAC 3 cut(s) 199, 257, 553
MnlI CCTC 9 cut(s) 48, 61, 67, 75, 180, 282, 657, 847, 976
Mph1103I ATGCAT 1 cut(s) 1017
MroI TCCGGA 1 cut(s) 844
MroXI GAANNNNTTC 1 cut(s) 1118
MseI TTAA 6 cut(s) 174, 264, 387, 519, 974, 1001
MspI CCGG 2 cut(s) 845, 980
MunI CAATTG 2 cut(s) 138, 1086
Mva1269I GAATGC 2 cut(s) 392, 986
MwoI GCNNNNNNNGC 4 cut(s) 335, 743, 937, 1025
NdeII GATC 4 cut(s) 235, 508, 567, 838
NlaIII CATG 3 cut(s) 531, 1015, 1019
NlaIV GGNNCC 5 cut(s) 23, 293, 631, 860, 955
NmeAIII GCCGAG 2 cut(s) 755, 1153
NmuCI GTSAC 1 cut(s) 553
NsiI ATGCAT 1 cut(s) 1017
NspI RCATGY 1 cut(s) 1019
PaeI GCATGC 1 cut(s) 1019
PaeR7I CTCGAG 1 cut(s) 1058
PctI GAATGC 2 cut(s) 392, 986
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 3 cut(s) 230, 282, 1114
PkrI GCNGC 8 cut(s) 47, 119, 158, 226, 502, 679, 748, 939
Ppu21I YACGTR 1 cut(s) 305
PsiI TTATAA 1 cut(s) 689
Psp124BI GAGCTC 1 cut(s) 776
Psp1406I AACGTT 1 cut(s) 881
PspFI CCCAGC 2 cut(s) 25, 503
PspN4I GGNNCC 5 cut(s) 23, 293, 631, 860, 955
PspPI GGNCC 5 cut(s) 81, 204, 291, 587, 859
PstI CTGCAG 3 cut(s) 47, 122, 212
PsuI RGATCY 2 cut(s) 508, 838
RsaI GTAC 3 cut(s) 134, 702, 1133
RsaNI GTAC 3 cut(s) 133, 701, 1132
SacI GAGCTC 1 cut(s) 776
SaqAI TTAA 6 cut(s) 174, 264, 387, 519, 974, 1001
SatI GCNGC 8 cut(s) 46, 118, 157, 225, 501, 678, 747, 938
Sau3AI GATC 4 cut(s) 235, 508, 567, 838
Sau96I GGNCC 5 cut(s) 81, 204, 291, 587, 859
SduI GDGCHC 2 cut(s) 776, 1059
SfaNI GCATC 2 cut(s) 259, 450
SfcI CTRYAG 4 cut(s) 43, 118, 208, 1099
Sfr274I CTCGAG 1 cut(s) 1058
SinI GGWCC 1 cut(s) 859
SlaI CTCGAG 1 cut(s) 1058
SmlI CTYRAG 3 cut(s) 112, 286, 1058
SmoI CTYRAG 3 cut(s) 112, 286, 1058
SphI GCATGC 1 cut(s) 1019
SsiI CCGC 4 cut(s) 19, 551, 650, 862
SspMI CTAG 2 cut(s) 741, 1153
SstI GAGCTC 1 cut(s) 776
StyI CCWWGG 4 cut(s) 625, 751, 919, 1077
TaaI ACNGT 3 cut(s) 313, 611, 700
TaiI ACGT 3 cut(s) 307, 884, 997
TaqI TCGA 1 cut(s) 1059
TatI WGTACW 2 cut(s) 132, 1131
TfiI GAWTC 3 cut(s) 230, 282, 1114
Tru1I TTAA 6 cut(s) 174, 264, 387, 519, 974, 1001
Tru9I TTAA 6 cut(s) 174, 264, 387, 519, 974, 1001
TscAI CASTG 2 cut(s) 47, 1035
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 8 cut(s) 45, 117, 156, 224, 500, 677, 746, 937
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 3 cut(s) 583, 674, 1138
TspRI CASTG 2 cut(s) 47, 1035
VpaK11BI GGWCC 1 cut(s) 859
XapI RAATTY 1 cut(s) 193
XceI RCATGY 1 cut(s) 1019
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 1058
XmiI GTMKAC 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 2 cut(s) 741, 1153
Zsp2I ATGCAT 1 cut(s) 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.