MD12G1066500.v1.1

Protein LST8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007389
Physical Location & Seq
Forward (+)
7848064 .. 7851740
3677 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1066500.v1.1.491

Sequence Viewer

Length: 981 bp
ATGTCTTTTTCTGAGGAGGTTAAGATGAGCCAGCAACCATCGGTGATCCTTGCAACGGCTAGCTATGATCACACTATTCGGTTTTGGGAGGCCAAAAGTGGCCGCTGCTACCGTACCATCCAGTACCCTGATTCGCAAGTTAATAGGCTTGAGATTACTCCAGATAAACGCTTCCTGGCTGCCGCTGGAAATCCTCACATTCGATTATTTGATGTTAATACAAATAGCCCTCAACCGGTGATGAGCTATGAGTCACATAGTAATAATGTAATGGCGGTAGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGTACAGTAAAAATTTGGGATTTGAGAGCCCCAGGTTGTCAAAGGGAATATGAAAGTCGTGCAGCTGTTAACACTGTTGTGCTTCACCCGAATCAGACTGAACTAATATCTGGGGACCAAAATGGCAATATTCGTGTTTGGGACTTGACAGCAAATTCATGCAGCTGTGAATTGGTTCCAGAGGTGGATACAGCTGTAAGGTCATTAACAGTTATGTGGGATGGGAGCTTGGTTGTTGCAGCAAATAATCATGGGACATGCTACGTTTGGCGCTTGTTGCGGGGAACCCAGACAATGACAGAGTTTGCACCACTTCATAAACTGCAAGCACACAAGGGATATTACATTCTTAAATGTCTGCTTTCACCTGAATTCTGCGAACCCCACAGATACTTGGCGACTGCCTCTTCGGACCACACTGTCAAGATATGGAATGTAGATGGTTTCACATTAGAGAAAACTTTAGTAGGTCATTCACGATGGGTTTGGGACTGTGTTTTCTCGGTAGATGGTGCCTATCTTATAACAGCTTCCTCTGATACAACAGCAAGGCTTTGGTCCATGTCCACCGGCGAAGATATCAGGGTGTATCAAGGGCATCACAAAGCAACCATTTGTTGTGCACTCCATGATGGAGCCGAACCTTCTGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006935 GO:0006950 GO:0007154 GO:0007165 GO:0007190 GO:0008064 GO:0008150 GO:0009267 GO:0009605 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0010638 GO:0016043 GO:0019220 GO:0019222 GO:0023052 GO:0030100 GO:0030587 GO:0030832 GO:0030833 GO:0030838 GO:0031152 GO:0031252 GO:0031323 GO:0031325 GO:0031334 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0031932 GO:0032147 GO:0032148 GO:0032268 GO:0032270 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040011 GO:0042221 GO:0042325 GO:0042327 GO:0042330 GO:0042594 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043327 GO:0043519 GO:0043520 GO:0043549 GO:0044087 GO:0044089 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045806 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0050764 GO:0050765 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051493 GO:0051495 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0071496 GO:0071840 GO:0080090 GO:0090066 GO:0090702 GO:0098630 GO:0098743 GO:0099120 GO:0110053 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04714 map04136 map04138 map04140 map04150 map04151 map04714
Pfam Domains
Protein Families
WD40

Protein Analysis

327

Amino Acids

36.51

Weight (kDa)

5.88

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 6 - 78 4.4e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 7 - 204 3.6e-30 WDR5 beta-propeller domain
WD40_Prp19 PF24814 7 - 122 4.5e-15 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 9 - 118 2.8e-10 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 72 1.4e-07 WDR3 first beta-propeller domain
EIF3I PF24805 13 - 120 1.4e-06 EIF3I
Beta-prop_EML PF23409 14 - 117 3.4e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 15 - 116 1.8e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 15 - 120 2.9e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 16 - 118 3.5e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 17 - 122 4.1e-13 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 21 - 124 6.4e-08 WDR36/Utp21 first beta-propeller
WDR55 PF24796 44 - 202 2.3e-16 WDR55
Beta-prop_THOC3 PF25174 45 - 316 4.9e-40 THOC3 beta-propeller domain
WD40_Gbeta PF25391 55 - 205 1e-13 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 57 - 256 1.6e-19 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 66 - 200 3.3e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 73 - 253 1.8e-19 Prp19 WD40 domain
WD40 PF00400 78 - 115 1.8e-10 WD domain, G-beta repeat
Beta-prop_WDR90_POC16_2nd PF23393 105 - 199 8.4e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 111 - 311 8e-19 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 128 - 295 1.2e-23 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 132 - 199 6.4e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 135 - 303 4.3e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 140 - 313 8.5e-16 TEP-1 second beta-propeller
WD40_Gbeta PF25391 176 - 315 2.8e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 232 - 318 1.5e-20 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 237 - 295 5.9e-06 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 237 - 310 5.8e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 237 - 318 1.2e-09 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 239 - 308 7.2e-10 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 240 - 310 1.9e-06 EIF3I
WD40 PF00400 259 - 295 1.8e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 848
AasI GACNNNNNNGTC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 836
AciI CCGC 4 cut(s) 103, 183, 275, 604
AclWI GGATC 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 3 cut(s) 336, 478, 695
AfaI GTAC 3 cut(s) 115, 125, 328
AfiI CCNNNNNNNGG 2 cut(s) 55, 235
AgeI ACCGGT 1 cut(s) 235
AgsI TTSAA 1 cut(s) 287
AjnI CCWGG 2 cut(s) 174, 355
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 7 cut(s) 63, 246, 389, 489, 518, 552, 854
AluI AGCT 7 cut(s) 63, 246, 389, 489, 518, 552, 854
Alw21I GWGCWC 1 cut(s) 949
Alw44I GTGCAC 1 cut(s) 945
AlwI GGATC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 2 cut(s) 90, 100
ApaLI GTGCAC 1 cut(s) 945
ApeKI GCWGC 5 cut(s) 105, 179, 386, 486, 563
ApoI RAATTY 3 cut(s) 336, 478, 695
ArsI GACNNNNNNTTYG 2 cut(s) 715, 747
AsiGI ACCGGT 1 cut(s) 235
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 597
AspS9I GGNCC 3 cut(s) 439, 736, 882
AsuHPI GGTGA 4 cut(s) 55, 250, 401, 681
AsuNHI GCTAGC 1 cut(s) 59
AvaII GGWCC 3 cut(s) 439, 736, 882
BaeGI GKGCMC 1 cut(s) 949
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BanI GGYRCC 1 cut(s) 836
BanII GRGCYC 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 949
BbvI GCAGC 5 cut(s) 92, 166, 398, 498, 575
BccI CCATC 9 cut(s) 46, 125, 287, 317, 539, 758, 798, 827, 950
BceAI ACGGC 1 cut(s) 72
BcgI CGANNNNNNTGC 1 cut(s) 953
BciT130I CCWGG 2 cut(s) 176, 357
BciVI GTATCC 1 cut(s) 505
BclI TGATCA 1 cut(s) 67
BfaI CTAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 598
BfuI GTATCC 1 cut(s) 505
BisI GCNGC 7 cut(s) 103, 106, 180, 183, 387, 487, 564
BlsI GCNGC 7 cut(s) 104, 107, 181, 184, 388, 488, 565
Bme1390I CCNGG 2 cut(s) 176, 357
Bme18I GGWCC 3 cut(s) 439, 736, 882
BmgT120I GGNCC 3 cut(s) 439, 736, 882
BmiI GGNNCC 5 cut(s) 440, 501, 610, 838, 961
BmrFI CCNGG 2 cut(s) 176, 357
BmsI GCATC 1 cut(s) 931
BmtI GCTAGC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 235
Bse118I RCCGGY 2 cut(s) 235, 893
Bse1I ACTGG 2 cut(s) 121, 305
BseBI CCWGG 2 cut(s) 176, 357
BseDI CCNNGG 1 cut(s) 355
BseGI GGATG 4 cut(s) 117, 309, 328, 550
BseLI CCNNNNNNNGG 2 cut(s) 55, 235
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 121, 305
BseRI GAGGAG 1 cut(s) 29
BseSI GKGCMC 1 cut(s) 949
BseXI GCAGC 5 cut(s) 92, 166, 398, 498, 575
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 2 cut(s) 92, 102
BshNI GGYRCC 1 cut(s) 836
BshTI ACCGGT 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 949
BsiSI CCGG 2 cut(s) 236, 894
BslFI GGGAC 4 cut(s) 452, 479, 592, 827
BslI CCNNNNNNNGG 2 cut(s) 55, 235
BsmFI GGGAC 4 cut(s) 452, 479, 592, 827
BsnI GGCC 2 cut(s) 92, 102
Bsp1286I GDGCHC 2 cut(s) 355, 949
Bsp143I GATC 2 cut(s) 45, 67
BspACI CCGC 4 cut(s) 103, 183, 275, 604
BspANI GGCC 2 cut(s) 92, 102
BspCNI CTCAG 2 cut(s) 4, 310
BspLI GGNNCC 5 cut(s) 440, 501, 610, 838, 961
BspOI GCTAGC 1 cut(s) 63
BspPI GGATC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 836
BsrFI RCCGGY 2 cut(s) 235, 893
BsrI ACTGG 2 cut(s) 121, 305
BssAI RCCGGY 2 cut(s) 235, 893
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 2 cut(s) 45, 67
Bst2UI CCWGG 2 cut(s) 176, 357
Bst4CI ACNGT 6 cut(s) 113, 331, 400, 535, 745, 818
Bst6I CTCTTC 1 cut(s) 736
BstC8I GCNNGC 3 cut(s) 32, 61, 651
BstDEI CTNAG 2 cut(s) 12, 318
BstF5I GGATG 4 cut(s) 117, 309, 328, 550
BstH2I RGCGCY 1 cut(s) 598
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 2 cut(s) 48, 70
BstMBI GATC 2 cut(s) 45, 67
BstMWI GCNNNNNNNGC 1 cut(s) 601
BstNI CCWGG 2 cut(s) 176, 357
BstNSI RCATGY 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 174, 355
BstSLI GKGCMC 1 cut(s) 949
BstV1I GCAGC 5 cut(s) 92, 166, 398, 498, 575
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 2 cut(s) 92, 102
BtsCI GGATG 4 cut(s) 117, 309, 328, 550
BtsIMutI CAGTG 2 cut(s) 396, 741
Cac8I GCNNGC 3 cut(s) 32, 61, 651
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 1 cut(s) 597
Cfr10I RCCGGY 2 cut(s) 235, 893
Cfr13I GGNCC 3 cut(s) 439, 736, 882
Csp6I GTAC 3 cut(s) 114, 124, 327
CspAI ACCGGT 1 cut(s) 235
CviAII CATG 5 cut(s) 483, 575, 582, 886, 953
CviQI GTAC 3 cut(s) 114, 124, 327
DdeI CTNAG 2 cut(s) 12, 318
DpnI GATC 2 cut(s) 47, 69
DpnII GATC 2 cut(s) 45, 67
DrdI GACNNNNNNGTC 1 cut(s) 743
DseDI GACNNNNNNGTC 1 cut(s) 743
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 736
EarI CTCTTC 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 355
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 3 cut(s) 439, 736, 882
EcoRI GAATTC 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 174, 355
EcoRV GATATC 1 cut(s) 904
EcoT38I GRGCYC 1 cut(s) 355
FaeI CATG 5 cut(s) 486, 578, 585, 889, 956
FaqI GGGAC 4 cut(s) 452, 479, 592, 827
FatI CATG 5 cut(s) 482, 574, 581, 885, 952
FauI CCCGC 1 cut(s) 597
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 7 cut(s) 103, 106, 180, 183, 387, 487, 564
FokI GGATG 4 cut(s) 104, 316, 335, 557
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 7 cut(s) 103, 106, 180, 183, 387, 487, 564
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 596
GluI GCNGC 7 cut(s) 103, 106, 180, 183, 387, 487, 564
GsuI CTGGAG 1 cut(s) 144
HaeII RGCGCY 1 cut(s) 598
HaeIII GGCC 2 cut(s) 92, 102
HapII CCGG 2 cut(s) 236, 894
HhaI GCGC 1 cut(s) 597
Hin1II CATG 5 cut(s) 486, 578, 585, 889, 956
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 3 cut(s) 131, 251, 415
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 2 cut(s) 236, 894
HphI GGTGA 4 cut(s) 55, 250, 401, 681
Hpy166II GTNNAC 3 cut(s) 394, 891, 947
Hpy188I TCNGA 5 cut(s) 13, 319, 420, 736, 862
Hpy188III TCNNGA 5 cut(s) 161, 503, 748, 801, 978
Hpy8I GTNNAC 3 cut(s) 394, 891, 947
HpyAV CCTTC 1 cut(s) 978
HpyCH4III ACNGT 6 cut(s) 113, 331, 400, 535, 745, 818
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 8 cut(s) 53, 386, 486, 563, 632, 649, 947, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 601
HpyF3I CTNAG 2 cut(s) 12, 318
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 5 cut(s) 486, 578, 585, 889, 956
HspAI GCGC 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 45, 67
LmnI GCTCC 2 cut(s) 549, 959
Lsp1109I GCAGC 5 cut(s) 92, 166, 398, 498, 575
LweI GCATC 1 cut(s) 931
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 1 cut(s) 252
MalI GATC 2 cut(s) 47, 69
MboI GATC 2 cut(s) 45, 67
MboII GAAGA 2 cut(s) 723, 911
MhlI GDGCHC 2 cut(s) 355, 949
MluCI AATT 4 cut(s) 336, 478, 494, 695
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 9 cut(s) 7, 10, 82, 204, 240, 313, 499, 739, 868
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 5 cut(s) 105, 185, 389, 489, 518
MspI CCGG 2 cut(s) 236, 894
MspR9I CCNGG 2 cut(s) 176, 357
MvaI CCWGG 2 cut(s) 176, 357
MwoI GCNNNNNNNGC 1 cut(s) 601
NdeII GATC 2 cut(s) 45, 67
NheI GCTAGC 1 cut(s) 59
NlaIII CATG 5 cut(s) 486, 578, 585, 889, 956
NlaIV GGNNCC 5 cut(s) 440, 501, 610, 838, 961
NmuCI GTSAC 1 cut(s) 252
NspI RCATGY 1 cut(s) 585
OliI CACNNNNGTG 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 2 cut(s) 131, 415
PinAI ACCGGT 1 cut(s) 235
PkrI GCNGC 7 cut(s) 104, 107, 181, 184, 388, 488, 565
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PsiI TTATAA 1 cut(s) 848
Psp6I CCWGG 2 cut(s) 174, 355
PspGI CCWGG 2 cut(s) 174, 355
PspN4I GGNNCC 5 cut(s) 440, 501, 610, 838, 961
PspPI GGNCC 3 cut(s) 439, 736, 882
PstNI CAGNNNCTG 1 cut(s) 317
PvuII CAGCTG 3 cut(s) 389, 489, 518
RsaI GTAC 3 cut(s) 115, 125, 328
RsaNI GTAC 3 cut(s) 114, 124, 327
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
SatI GCNGC 7 cut(s) 103, 106, 180, 183, 387, 487, 564
Sau3AI GATC 2 cut(s) 45, 67
Sau96I GGNCC 3 cut(s) 439, 736, 882
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 176, 357
SduI GDGCHC 2 cut(s) 355, 949
SfaNI GCATC 1 cut(s) 931
SgrAI CRCCGGYG 1 cut(s) 893
SinI GGWCC 3 cut(s) 439, 736, 882
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 4 cut(s) 336, 478, 494, 695
SsiI CCGC 4 cut(s) 103, 183, 275, 604
SspI AATATT 1 cut(s) 454
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 174, 355
TaaI ACNGT 6 cut(s) 113, 331, 400, 535, 745, 818
TaiI ACGT 1 cut(s) 591
TaqI TCGA 1 cut(s) 202
TasI AATT 4 cut(s) 336, 478, 494, 695
TauI GCSGC 2 cut(s) 105, 185
TfiI GAWTC 2 cut(s) 131, 415
Tru1I TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
Tru9I TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
TscAI CASTG 2 cut(s) 403, 748
TseFI GTSAC 1 cut(s) 252
TseI GCWGC 5 cut(s) 105, 179, 386, 486, 563
Tsp45I GTSAC 1 cut(s) 252
TspDTI ATGAA 3 cut(s) 390, 471, 629
TspRI CASTG 2 cut(s) 403, 748
VneI GTGCAC 1 cut(s) 945
VpaK11BI GGWCC 3 cut(s) 439, 736, 882
XapI RAATTY 3 cut(s) 336, 478, 695
XceI RCATGY 1 cut(s) 585
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.