pycom12g05790

Protein LST8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007389
Physical Location & Seq
Forward (+)
5780338 .. 5783690
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05790.10

Sequence Viewer

Length: 981 bp
ATGTCAACTCAGGAGGATGTTAAGATGAGCCAGCAACCATCGGTGATCCTTGCAACGGCTAGCTATGATCACACTATTCGGTTTTGGGAGGCCAAAAGTGGCCGCTGCTACCGTACCATCCAGTGCCCTGATTCGCAAGTTAATAGGCTTGAGATTACTCCAGATAAACGCTTCCTGGCTGCAGCCGGAAATCCTCACATTCGATTATTTGATGTTAATACAAATAACCCTCAACCGGTGATGAGCTATGAGTCACATAGTAATAATGTAATGGCAGTAGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGTACTGTAAAAATTTGGGATTTGAGAGCCCCAGGTTGTCAAAGGGAATATGAAAGTCGTGCAGCTGTTAACACTGTTGTGCTTCACCCGAATCAGACTGAACTAATATCTGGGGACCAAAATGGCAATATTCGTGTTTGGGACTTGACAGCAAATTCATGCAGCTGTGAATTGGTTCCAGAGGTGGATACGGCTGTAAGGTCATTAACAGTTATGTGGGATGGGAGCTTGGTTGTTGCAGCAAATAATCATGGGACATGCTACGTTTGGCGCTTGCTGCGGGGAACCCAGACAATGACCGAGTTTGCGCCACTTCATAAACTGCAAGCACACAAGGGAAGTTACATTCTTAAATGTCTTCTTTCACCTGAATTCTGCGAACCCCACAGATACTTGGCGACTGCCTCTTCGGACCACACTGTCAAGATATGGAATGTAGATGGTTTCACATTAGAGAAAACTTTAGTAGGACATGAACGATGGGTGTGGGACTGTGTTTTCTCAGTAGACGGTGCCTATCTTATAACAGCTTCCTCTGATGCAACAGCAAGGCTTTGGTCCATGTCCACCGGCGAAGATATCAGGGTGTATCAAGGGCATCACAAAGCAACCATTTGTTGTGCACTCCATGATGGAGCCGAACCTTCTGCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006935 GO:0006950 GO:0007154 GO:0007165 GO:0007190 GO:0008064 GO:0008150 GO:0009267 GO:0009605 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0010638 GO:0016043 GO:0019220 GO:0019222 GO:0023052 GO:0030100 GO:0030587 GO:0030832 GO:0030833 GO:0030838 GO:0031152 GO:0031252 GO:0031323 GO:0031325 GO:0031334 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0031932 GO:0032147 GO:0032148 GO:0032268 GO:0032270 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040011 GO:0042221 GO:0042325 GO:0042327 GO:0042330 GO:0042594 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043327 GO:0043519 GO:0043520 GO:0043549 GO:0044087 GO:0044089 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045806 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0050764 GO:0050765 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051493 GO:0051495 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0071496 GO:0071840 GO:0080090 GO:0090066 GO:0090702 GO:0098630 GO:0098743 GO:0099120 GO:0110053 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04714 map04136 map04138 map04140 map04150 map04151 map04714
Pfam Domains
Protein Families
WD40

Protein Analysis

327

Amino Acids

36.39

Weight (kDa)

5.78

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 848
AasI GACNNNNNNGTC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 836
AccI GTMKAC 1 cut(s) 831
AciI CCGC 2 cut(s) 103, 604
AclWI GGATC 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 3 cut(s) 336, 478, 695
AfaI GTAC 2 cut(s) 115, 328
AfiI CCNNNNNNNGG 2 cut(s) 55, 235
AgeI ACCGGT 1 cut(s) 235
AgsI TTSAA 1 cut(s) 287
AjnI CCWGG 2 cut(s) 174, 355
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 6 cut(s) 63, 246, 389, 489, 552, 854
AluI AGCT 6 cut(s) 63, 246, 389, 489, 552, 854
Alw21I GWGCWC 1 cut(s) 949
Alw44I GTGCAC 1 cut(s) 945
AlwI GGATC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 2 cut(s) 90, 100
ApaLI GTGCAC 1 cut(s) 945
ApeKI GCWGC 7 cut(s) 105, 179, 182, 386, 486, 563, 601
ApoI RAATTY 3 cut(s) 336, 478, 695
ArsI GACNNNNNNTTYG 2 cut(s) 715, 747
AsiGI ACCGGT 1 cut(s) 235
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 2 cut(s) 597, 634
AspS9I GGNCC 3 cut(s) 439, 736, 882
AsuHPI GGTGA 4 cut(s) 55, 250, 401, 681
AsuNHI GCTAGC 1 cut(s) 59
AvaII GGWCC 3 cut(s) 439, 736, 882
BaeGI GKGCMC 2 cut(s) 128, 949
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BanI GGYRCC 1 cut(s) 836
BanII GRGCYC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 949
BbvI GCAGC 7 cut(s) 92, 166, 194, 398, 498, 575, 588
BccI CCATC 8 cut(s) 46, 125, 287, 317, 539, 758, 798, 950
BceAI ACGGC 2 cut(s) 72, 531
BcgI CGANNNNNNTGC 1 cut(s) 953
BciT130I CCWGG 2 cut(s) 176, 357
BciVI GTATCC 1 cut(s) 505
BclI TGATCA 1 cut(s) 67
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 598
BfuI GTATCC 1 cut(s) 505
BisI GCNGC 8 cut(s) 103, 106, 180, 183, 387, 487, 564, 602
BlsI GCNGC 8 cut(s) 104, 107, 181, 184, 388, 488, 565, 603
Bme1390I CCNGG 2 cut(s) 176, 357
Bme18I GGWCC 3 cut(s) 439, 736, 882
BmgT120I GGNCC 3 cut(s) 439, 736, 882
BmiI GGNNCC 5 cut(s) 440, 501, 610, 838, 961
BmrFI CCNGG 2 cut(s) 176, 357
BmsI GCATC 2 cut(s) 853, 931
BmtI GCTAGC 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 674
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 235
Bse118I RCCGGY 2 cut(s) 235, 893
Bse1I ACTGG 2 cut(s) 121, 305
BseBI CCWGG 2 cut(s) 176, 357
BseDI CCNNGG 1 cut(s) 355
BseGI GGATG 5 cut(s) 22, 117, 309, 328, 550
BseLI CCNNNNNNNGG 2 cut(s) 55, 235
BseMII CTCAG 3 cut(s) 23, 309, 840
BseNI ACTGG 2 cut(s) 121, 305
BseSI GKGCMC 2 cut(s) 128, 949
BseXI GCAGC 7 cut(s) 92, 166, 194, 398, 498, 575, 588
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 2 cut(s) 92, 102
BshNI GGYRCC 1 cut(s) 836
BshTI ACCGGT 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 949
BsiSI CCGG 3 cut(s) 186, 236, 894
BslFI GGGAC 4 cut(s) 452, 479, 592, 827
BslI CCNNNNNNNGG 2 cut(s) 55, 235
BsmFI GGGAC 4 cut(s) 452, 479, 592, 827
BsnI GGCC 2 cut(s) 92, 102
Bsp1286I GDGCHC 3 cut(s) 128, 355, 949
Bsp143I GATC 2 cut(s) 45, 67
BspACI CCGC 2 cut(s) 103, 604
BspANI GGCC 2 cut(s) 92, 102
BspCNI CTCAG 3 cut(s) 22, 310, 839
BspLI GGNNCC 5 cut(s) 440, 501, 610, 838, 961
BspMAI CTGCAG 1 cut(s) 184
BspOI GCTAGC 1 cut(s) 63
BspPI GGATC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 836
BsrFI RCCGGY 2 cut(s) 235, 893
BsrI ACTGG 2 cut(s) 121, 305
BssAI RCCGGY 2 cut(s) 235, 893
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 2 cut(s) 45, 67
Bst2UI CCWGG 2 cut(s) 176, 357
Bst4CI ACNGT 7 cut(s) 113, 331, 400, 535, 745, 818, 836
Bst6I CTCTTC 1 cut(s) 736
BstC8I GCNNGC 4 cut(s) 32, 61, 599, 651
BstDEI CTNAG 3 cut(s) 9, 318, 826
BstF5I GGATG 5 cut(s) 22, 117, 309, 328, 550
BstH2I RGCGCY 1 cut(s) 598
BstHHI GCGC 2 cut(s) 597, 634
BstKTI GATC 2 cut(s) 48, 70
BstMBI GATC 2 cut(s) 45, 67
BstMWI GCNNNNNNNGC 1 cut(s) 601
BstNI CCWGG 2 cut(s) 176, 357
BstNSI RCATGY 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 174, 355
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 2 cut(s) 128, 949
BstV1I GCAGC 7 cut(s) 92, 166, 194, 398, 498, 575, 588
BstV2I GAAGAC 1 cut(s) 674
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 2 cut(s) 92, 102
BtsCI GGATG 5 cut(s) 22, 117, 309, 328, 550
BtsIMutI CAGTG 3 cut(s) 128, 396, 741
Cac8I GCNNGC 4 cut(s) 32, 61, 599, 651
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 2 cut(s) 597, 634
Cfr10I RCCGGY 2 cut(s) 235, 893
Cfr13I GGNCC 3 cut(s) 439, 736, 882
Csp6I GTAC 2 cut(s) 114, 327
CspAI ACCGGT 1 cut(s) 235
CviAII CATG 6 cut(s) 483, 575, 582, 797, 886, 953
CviQI GTAC 2 cut(s) 114, 327
DdeI CTNAG 3 cut(s) 9, 318, 826
DpnI GATC 2 cut(s) 47, 69
DpnII GATC 2 cut(s) 45, 67
DrdI GACNNNNNNGTC 1 cut(s) 743
DseDI GACNNNNNNGTC 1 cut(s) 743
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 736
EarI CTCTTC 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 355
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 3 cut(s) 439, 736, 882
EcoRI GAATTC 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 174, 355
EcoRV GATATC 1 cut(s) 904
EcoT38I GRGCYC 1 cut(s) 355
FaeI CATG 6 cut(s) 486, 578, 585, 800, 889, 956
FaqI GGGAC 4 cut(s) 452, 479, 592, 827
FatI CATG 6 cut(s) 482, 574, 581, 796, 885, 952
FauI CCCGC 1 cut(s) 597
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 8 cut(s) 103, 106, 180, 183, 387, 487, 564, 602
FokI GGATG 5 cut(s) 29, 104, 316, 335, 557
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 8 cut(s) 103, 106, 180, 183, 387, 487, 564, 602
FspBI CTAG 1 cut(s) 60
GlaI GCGC 2 cut(s) 596, 633
GluI GCNGC 8 cut(s) 103, 106, 180, 183, 387, 487, 564, 602
GsuI CTGGAG 1 cut(s) 144
HaeII RGCGCY 1 cut(s) 598
HaeIII GGCC 2 cut(s) 92, 102
HapII CCGG 3 cut(s) 186, 236, 894
HhaI GCGC 2 cut(s) 597, 634
Hin1II CATG 6 cut(s) 486, 578, 585, 800, 889, 956
Hin6I GCGC 2 cut(s) 595, 632
HinP1I GCGC 2 cut(s) 595, 632
HincII GTYRAC 2 cut(s) 6, 394
HindII GTYRAC 2 cut(s) 6, 394
HinfI GANTC 3 cut(s) 131, 251, 415
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 3 cut(s) 186, 236, 894
HphI GGTGA 4 cut(s) 55, 250, 401, 681
Hpy166II GTNNAC 5 cut(s) 6, 394, 832, 891, 947
Hpy188I TCNGA 4 cut(s) 319, 420, 736, 862
Hpy188III TCNNGA 5 cut(s) 11, 161, 503, 748, 978
Hpy8I GTNNAC 5 cut(s) 6, 394, 832, 891, 947
HpyAV CCTTC 1 cut(s) 978
HpyCH4III ACNGT 7 cut(s) 113, 331, 400, 535, 745, 818, 836
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 9 cut(s) 53, 182, 386, 486, 563, 649, 866, 947, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 601
HpyF3I CTNAG 3 cut(s) 9, 318, 826
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 6 cut(s) 486, 578, 585, 800, 889, 956
HspAI GCGC 2 cut(s) 595, 632
Ksp22I TGATCA 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 45, 67
LmnI GCTCC 2 cut(s) 549, 959
Lsp1109I GCAGC 7 cut(s) 92, 166, 194, 398, 498, 575, 588
LweI GCATC 2 cut(s) 853, 931
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 2 cut(s) 252, 665
MalI GATC 2 cut(s) 47, 69
MboI GATC 2 cut(s) 45, 67
MboII GAAGA 3 cut(s) 674, 723, 911
MhlI GDGCHC 3 cut(s) 128, 355, 949
MluCI AATT 4 cut(s) 336, 478, 494, 695
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 8 cut(s) 7, 82, 204, 240, 313, 499, 739, 868
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 3 cut(s) 105, 389, 489
MspI CCGG 3 cut(s) 186, 236, 894
MspR9I CCNGG 2 cut(s) 176, 357
MvaI CCWGG 2 cut(s) 176, 357
MwoI GCNNNNNNNGC 1 cut(s) 601
NdeII GATC 2 cut(s) 45, 67
NheI GCTAGC 1 cut(s) 59
NlaIII CATG 6 cut(s) 486, 578, 585, 800, 889, 956
NlaIV GGNNCC 5 cut(s) 440, 501, 610, 838, 961
NmuCI GTSAC 1 cut(s) 252
NspI RCATGY 1 cut(s) 585
OliI CACNNNNGTG 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 2 cut(s) 131, 415
PinAI ACCGGT 1 cut(s) 235
PkrI GCNGC 8 cut(s) 104, 107, 181, 184, 388, 488, 565, 603
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PsiI TTATAA 1 cut(s) 848
Psp6I CCWGG 2 cut(s) 174, 355
PspGI CCWGG 2 cut(s) 174, 355
PspN4I GGNNCC 5 cut(s) 440, 501, 610, 838, 961
PspPI GGNCC 3 cut(s) 439, 736, 882
PstI CTGCAG 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 317
PvuII CAGCTG 2 cut(s) 389, 489
RsaI GTAC 2 cut(s) 115, 328
RsaNI GTAC 2 cut(s) 114, 327
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
SatI GCNGC 8 cut(s) 103, 106, 180, 183, 387, 487, 564, 602
Sau3AI GATC 2 cut(s) 45, 67
Sau96I GGNCC 3 cut(s) 439, 736, 882
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 176, 357
SduI GDGCHC 3 cut(s) 128, 355, 949
SfaNI GCATC 2 cut(s) 853, 931
SfcI CTRYAG 1 cut(s) 180
SgrAI CRCCGGYG 1 cut(s) 893
SinI GGWCC 3 cut(s) 439, 736, 882
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 4 cut(s) 336, 478, 494, 695
SsiI CCGC 2 cut(s) 103, 604
SspI AATATT 1 cut(s) 454
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 174, 355
TaaI ACNGT 7 cut(s) 113, 331, 400, 535, 745, 818, 836
TaiI ACGT 1 cut(s) 591
TaqI TCGA 1 cut(s) 202
TaqII GACCGA 1 cut(s) 638
TasI AATT 4 cut(s) 336, 478, 494, 695
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 2 cut(s) 131, 415
Tru1I TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
Tru9I TTAA 6 cut(s) 21, 141, 216, 393, 530, 675
TscAI CASTG 3 cut(s) 128, 403, 748
TseFI GTSAC 1 cut(s) 252
TseI GCWGC 7 cut(s) 105, 179, 182, 386, 486, 563, 601
Tsp45I GTSAC 1 cut(s) 252
TspDTI ATGAA 4 cut(s) 390, 471, 629, 813
TspRI CASTG 3 cut(s) 128, 403, 748
VneI GTGCAC 1 cut(s) 945
VpaK11BI GGWCC 3 cut(s) 439, 736, 882
XapI RAATTY 3 cut(s) 336, 478, 695
XceI RCATGY 1 cut(s) 585
XmiI GTMKAC 1 cut(s) 831
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.