Rroxscaffold_6G00400740

Protein LST8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007389
Physical Location & Seq
Reverse (-)
22886183 .. 22889907
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400740.1

Sequence Viewer

Length: 894 bp
ATGAGCCAACAACCGTCGGTGATACTTGCGACGGCGAGCTATGACCACACTATTCGCTTCTGGGAGGCCAAAAGTGGCCGCTGCTACCGTACCATCCAATACCCTGATTCACAAGTAAATAGGCTTGAGATAACCCCGGATAAACGATTCCTGGCTGCAGCGGGCAACCCTCATATTCGATTGTTTGATGTTAATTCAAACAGTCCTCAACCGGTGATGAGCTATGATTCACATACTAATAACGTAATGGCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCCGAGGATGGTACAGTAAAAATTTGGGATTTGAGAGCTCCAGGTTGCCAAAGGGAATATGAAAGCCGTGCGGCTGTTAACACTGTTGTGCTGCACCCGAATCAGACTGAACTAATATCTGGGGACCAAAATGGCAACATTCGTGTTTGGGATTTGACAGCTAATTCTTGCAGTTGTGAATTGGTTCCAGAGGTGGATACTGCTGTAAGGTCGTTAACAGTTATGTGGGATGGGAGCTTGGTCGTTGCTGCAAATAATCATGGGACATGCTATGTTTGGCGCTTGTTGCGAGGGACACAGACTATGACAAATTTTGAGCCACTTCATAAGCTTCAAGCACACAAGGGATACATCCTCAAGTGTCTACTTTCACCTGAGTTCTGTGAACCCCACAGATATCTGGCCACTGCCTCTTCTGACCACACTGTCAAGATATGGAATGTTGATGGTTTCACATTAGAGAAAACTCTAGAAGCTTCCTCTGATACAACAGCAAGGCTTTGGAACCTGTCCACCGGAGAAGATATCAGGGTGTATCAGGGGCATCACAAAGCAACCATTTGTTGTGCACTGCATGATGGAGCTGAACCTGGTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006935 GO:0006950 GO:0007154 GO:0007165 GO:0007190 GO:0008064 GO:0008150 GO:0009267 GO:0009605 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0010638 GO:0016043 GO:0019220 GO:0019222 GO:0023052 GO:0030100 GO:0030587 GO:0030832 GO:0030833 GO:0030838 GO:0031152 GO:0031252 GO:0031323 GO:0031325 GO:0031334 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0031932 GO:0032147 GO:0032148 GO:0032268 GO:0032270 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040011 GO:0042221 GO:0042325 GO:0042327 GO:0042330 GO:0042594 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043327 GO:0043519 GO:0043520 GO:0043549 GO:0044087 GO:0044089 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045806 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0050764 GO:0050765 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051493 GO:0051495 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0071496 GO:0071840 GO:0080090 GO:0090066 GO:0090702 GO:0098630 GO:0098743 GO:0099120 GO:0110053 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04714 map04136 map04138 map04140 map04150 map04151 map04714
Pfam Domains
Protein Families
WD40

Protein Analysis

297

Amino Acids

33.18

Weight (kDa)

5.93

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 6 - 109 6.2e-06 EIF3I
Beta-prop_WDR3_1st PF25173 7 - 64 2.5e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 7 - 196 1.5e-29 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 7 - 66 6.8e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 7 - 110 2e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 8 - 111 3.1e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 8 - 110 6.1e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 8 - 126 6.7e-13 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 9 - 114 1e-12 WDHD1 first WD40 domain
Beta-prop_EML PF23409 10 - 108 9.8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 13 - 116 1.5e-07 WDR36/Utp21 first beta-propeller
WDR55 PF24796 36 - 195 2.2e-16 WDR55
Beta-prop_THOC3 PF25174 37 - 255 2.5e-29 THOC3 beta-propeller domain
WD40_Gbeta PF25391 48 - 198 2.6e-13 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 49 - 285 7.5e-23 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 58 - 192 3.5e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 60 - 244 3.5e-20 Prp19 WD40 domain
WD40 PF00400 70 - 107 1.5e-10 WD domain, G-beta repeat
Beta-prop_WDR90_POC16_2nd PF23393 97 - 191 7.2e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st PF25173 119 - 248 5.5e-15 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 123 - 248 2.1e-06 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 124 - 191 4.6e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 127 - 246 1.8e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 132 - 246 1.3e-08 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 655
AciI CCGC 3 cut(s) 79, 161, 362
AcoI YGGCCR 2 cut(s) 76, 693
AcsI RAATTY 2 cut(s) 312, 601
AfaI GTAC 2 cut(s) 91, 304
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 3 cut(s) 198, 263, 626
AjnI CCWGG 3 cut(s) 150, 331, 880
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 8 cut(s) 39, 222, 329, 452, 528, 622, 767, 875
AluI AGCT 8 cut(s) 39, 222, 329, 452, 528, 622, 767, 875
Alw21I GWGCWC 2 cut(s) 331, 862
Alw44I GTGCAC 1 cut(s) 858
AoxI GGCC 3 cut(s) 66, 76, 693
ApaLI GTGCAC 1 cut(s) 858
ApeKI GCWGC 5 cut(s) 81, 155, 158, 382, 539
ApoI RAATTY 2 cut(s) 312, 601
AsiGI ACCGGT 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 474
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 2 cut(s) 415, 884
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 31, 226, 654
AvaII GGWCC 2 cut(s) 415, 884
BaeGI GKGCMC 1 cut(s) 862
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BalI TGGCCA 1 cut(s) 695
BanII GRGCYC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 331, 862
BbvI GCAGC 5 cut(s) 68, 142, 170, 369, 526
BccI CCATC 6 cut(s) 101, 263, 293, 515, 731, 863
BceAI ACGGC 2 cut(s) 48, 342
BciT130I CCWGG 3 cut(s) 152, 333, 882
BciVI GTATCC 2 cut(s) 481, 632
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 574
BfuI GTATCC 2 cut(s) 481, 632
BisI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
BlsI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
Bme1390I CCNGG 4 cut(s) 137, 152, 333, 882
Bme18I GGWCC 2 cut(s) 415, 884
BmgT120I GGNCC 2 cut(s) 415, 884
BmiI GGNNCC 4 cut(s) 292, 416, 477, 797
BmrFI CCNGG 4 cut(s) 137, 152, 333, 882
BmsI GCATC 1 cut(s) 844
BpmI CTGGAG 1 cut(s) 315
BpuEI CTTGAG 2 cut(s) 146, 632
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 135, 294
BsaWI WCCGGW 2 cut(s) 211, 806
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 281
BseBI CCWGG 3 cut(s) 152, 333, 882
BseDI CCNNGG 2 cut(s) 135, 294
BseGI GGATG 5 cut(s) 93, 285, 304, 526, 642
BseMII CTCAG 1 cut(s) 657
BseNI ACTGG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 862
BseXI GCAGC 5 cut(s) 68, 142, 170, 369, 526
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 3 cut(s) 68, 78, 695
BshTI ACCGGT 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 331, 862
BsiSI CCGG 3 cut(s) 137, 212, 807
BslFI GGGAC 3 cut(s) 428, 568, 598
BsmFI GGGAC 3 cut(s) 428, 568, 598
BsnI GGCC 3 cut(s) 68, 78, 695
Bsp1286I GDGCHC 2 cut(s) 331, 862
BspACI CCGC 3 cut(s) 79, 161, 362
BspANI GGCC 3 cut(s) 68, 78, 695
BspCNI CTCAG 1 cut(s) 658
BspLI GGNNCC 4 cut(s) 292, 416, 477, 797
BspMAI CTGCAG 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 2 cut(s) 135, 294
Bst2UI CCWGG 3 cut(s) 152, 333, 882
Bst4CI ACNGT 7 cut(s) 15, 89, 203, 307, 376, 511, 718
Bst6I CTCTTC 1 cut(s) 709
BstC8I GCNNGC 2 cut(s) 37, 163
BstDEI CTNAG 1 cut(s) 666
BstF5I GGATG 5 cut(s) 93, 285, 304, 526, 642
BstH2I RGCGCY 1 cut(s) 574
BstHHI GCGC 1 cut(s) 573
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstNI CCWGG 3 cut(s) 152, 333, 882
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 4 cut(s) 135, 150, 331, 880
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 862
BstV1I GCAGC 5 cut(s) 68, 142, 170, 369, 526
BsuI GTATCC 2 cut(s) 481, 632
BsuRI GGCC 3 cut(s) 68, 78, 695
BtsCI GGATG 5 cut(s) 93, 285, 304, 526, 642
BtsI GCAGTG 3 cut(s) 258, 696, 860
BtsIMutI CAGTG 5 cut(s) 258, 372, 696, 714, 860
Cac8I GCNNGC 2 cut(s) 37, 163
CfoI GCGC 1 cut(s) 573
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 415, 884
CsiI ACCWGGT 1 cut(s) 880
Csp6I GTAC 2 cut(s) 90, 303
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 3 cut(s) 551, 558, 866
CviQI GTAC 2 cut(s) 90, 303
DdeI CTNAG 1 cut(s) 666
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 2 cut(s) 76, 693
Eam1104I CTCTTC 1 cut(s) 709
EarI CTCTTC 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 331
Eco32I GATATC 2 cut(s) 689, 817
Eco47I GGWCC 2 cut(s) 415, 884
Eco53kI GAGCTC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 329
EcoRII CCWGG 3 cut(s) 150, 331, 880
EcoRV GATATC 2 cut(s) 689, 817
EcoT38I GRGCYC 1 cut(s) 331
FaeI CATG 3 cut(s) 554, 561, 869
FaqI GGGAC 3 cut(s) 428, 568, 598
FatI CATG 3 cut(s) 550, 557, 865
FauI CCCGC 1 cut(s) 154
FblI GTMKAC 1 cut(s) 655
Fnu4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FokI GGATG 5 cut(s) 80, 292, 311, 533, 629
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FspBI CTAG 1 cut(s) 761
GlaI GCGC 1 cut(s) 572
GluI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
GsuI CTGGAG 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 574
HaeIII GGCC 3 cut(s) 68, 78, 695
HapII CCGG 3 cut(s) 137, 212, 807
HhaI GCGC 1 cut(s) 573
Hin1II CATG 3 cut(s) 554, 561, 869
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HincII GTYRAC 2 cut(s) 370, 507
HindII GTYRAC 2 cut(s) 370, 507
HindIII AAGCTT 2 cut(s) 620, 765
HinfI GANTC 4 cut(s) 107, 147, 227, 391
HpaI GTTAAC 2 cut(s) 370, 507
HpaII CCGG 3 cut(s) 137, 212, 807
HphI GGTGA 3 cut(s) 31, 226, 654
Hpy166II GTNNAC 6 cut(s) 370, 507, 656, 677, 804, 860
Hpy188I TCNGA 4 cut(s) 295, 396, 709, 775
Hpy188III TCNNGA 4 cut(s) 479, 721, 761, 891
Hpy8I GTNNAC 6 cut(s) 370, 507, 656, 677, 804, 860
Hpy99I CGWCG 2 cut(s) 19, 34
HpyCH4III ACNGT 7 cut(s) 15, 89, 203, 307, 376, 511, 718
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 6 cut(s) 158, 385, 462, 542, 860, 865
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 1 cut(s) 666
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 3 cut(s) 554, 561, 869
HspAI GCGC 1 cut(s) 571
KspAI GTTAAC 2 cut(s) 370, 507
LmnI GCTCC 3 cut(s) 334, 525, 872
Lsp1109I GCAGC 5 cut(s) 68, 142, 170, 369, 526
LweI GCATC 1 cut(s) 844
MabI ACCWGGT 1 cut(s) 880
MaeI CTAG 1 cut(s) 761
MaeII ACGT 1 cut(s) 243
MboII GAAGA 2 cut(s) 696, 824
MhlI GDGCHC 2 cut(s) 331, 862
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 5 cut(s) 193, 312, 454, 470, 601
MluNI TGGCCA 1 cut(s) 695
MnlI CCTC 9 cut(s) 58, 180, 216, 289, 475, 575, 656, 712, 781
Mox20I TGGCCA 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 695
MseI TTAA 3 cut(s) 192, 369, 506
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 695
MspA1I CMGCKG 2 cut(s) 81, 161
MspI CCGG 3 cut(s) 137, 212, 807
MspR9I CCNGG 4 cut(s) 137, 152, 333, 882
MvaI CCWGG 3 cut(s) 152, 333, 882
MwoI GCNNNNNNNGC 1 cut(s) 577
NciI CCSGG 1 cut(s) 137
NlaIII CATG 3 cut(s) 554, 561, 869
NlaIV GGNNCC 4 cut(s) 292, 416, 477, 797
NspI RCATGY 1 cut(s) 561
OliI CACNNNNGTG 1 cut(s) 377
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 4 cut(s) 107, 147, 227, 391
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 3 cut(s) 150, 331, 880
PspGI CCWGG 3 cut(s) 150, 331, 880
PspN4I GGNNCC 4 cut(s) 292, 416, 477, 797
PspPI GGNCC 2 cut(s) 415, 884
PstI CTGCAG 1 cut(s) 160
RsaI GTAC 2 cut(s) 91, 304
RsaNI GTAC 2 cut(s) 90, 303
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 192, 369, 506
SatI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
Sau96I GGNCC 2 cut(s) 415, 884
ScrFI CCNGG 4 cut(s) 137, 152, 333, 882
SduI GDGCHC 2 cut(s) 331, 862
SexAI ACCWGGT 1 cut(s) 880
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 2 cut(s) 415, 884
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 125, 647
SmoI CTYRAG 2 cut(s) 125, 647
Sse9I AATT 5 cut(s) 193, 312, 454, 470, 601
SsiI CCGC 3 cut(s) 79, 161, 362
SspMI CTAG 1 cut(s) 761
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 4 cut(s) 135, 150, 331, 880
TaaI ACNGT 7 cut(s) 15, 89, 203, 307, 376, 511, 718
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 178
TasI AATT 5 cut(s) 193, 312, 454, 470, 601
TauI GCSGC 2 cut(s) 81, 365
TfiI GAWTC 4 cut(s) 107, 147, 227, 391
Tru1I TTAA 3 cut(s) 192, 369, 506
Tru9I TTAA 3 cut(s) 192, 369, 506
TscAI CASTG 5 cut(s) 258, 379, 703, 721, 867
TseI GCWGC 5 cut(s) 81, 155, 158, 382, 539
TspDTI ATGAA 2 cut(s) 366, 605
TspRI CASTG 5 cut(s) 258, 379, 703, 721, 867
VneI GTGCAC 1 cut(s) 858
VpaK11BI GGWCC 2 cut(s) 415, 884
XapI RAATTY 2 cut(s) 312, 601
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 655
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.