Rh3BG275000

Protein LST8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0007389
Physical Location & Seq
Forward (+)
26877548 .. 26880989
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG275000.1

Sequence Viewer

Length: 957 bp
ATGAGCCAACAACCGTCGGTGATACTTGCGACGGCGAGCTATGACCACACTATTCGCTTCTGGGAGGCCAAAAGTGGCCGCTGCTACCGTACCATCCAATACCCTGATTCACAAGTAAATAGGCTTGAGATAACCCCGGATAAACGATTCCTGGCTGCAGCGGGCAACCCTCATATTCGATTGTTTGATGTTAATTCAAACAGTCCTCAACCGGTGATGAGCTATGATTCACATACTAATAACGTAATGGCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCCGAGGATGGTACAGTAAAAATTTGGGATTTGAGAGCTCCAGGTTGCCAAAGGGAATATGAAAGCCGTGCAGCTGTTAACACTGTTGTGCTGCACCCGAATCAGACTGAACTAATATCTGGGGACCAAAATGGCAACATTCGTGTTTGGGATTTGACAGCGAATTCTTGCAGTTGTGAATTGGTTCCAGAGGTAGATACTGCTGTAAGGTCGTTAACAGTTATGTGGGATGGGAGCTTGGTTGTTGCTGCAAATAATCATGGGACATGCTATGTTTGGCGCTTGTTGCGAGGGACACAGACTATGACAAATTTTGAGCCACTTCATAAGCTTCAAGCACACACGGGATACAACATCCTCAAATGTCTACTTTCACCTGAGTTCTGTGAACCCCACAGATATTTGGCCACTGCCTCTTCTGACCACACTGTCAAGATATGGAATGTTGATGGTTTCACATTAGAGAAAACTCTAGAAGGGCATCAACGGTGGGTTTGGGACTGTGTTTTTTCCGTAGATGGTGCCTATCTTATAACAGCTTCCTCTGATTCAACAGCAAGGCTTTGGAACCTGTCCACCGGAGAAGATATCAGGGTGTATCAGGGGCATCACAAAGCAACCATTTGTTGTGCACTGCATGATGGAGCTGAACCTGGTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006935 GO:0006950 GO:0007154 GO:0007165 GO:0007190 GO:0008064 GO:0008150 GO:0009267 GO:0009605 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0010638 GO:0016043 GO:0019220 GO:0019222 GO:0023052 GO:0030100 GO:0030587 GO:0030832 GO:0030833 GO:0030838 GO:0031152 GO:0031252 GO:0031323 GO:0031325 GO:0031334 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0031932 GO:0032147 GO:0032148 GO:0032268 GO:0032270 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040011 GO:0042221 GO:0042325 GO:0042327 GO:0042330 GO:0042594 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043327 GO:0043519 GO:0043520 GO:0043549 GO:0044087 GO:0044089 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045806 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0050764 GO:0050765 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051493 GO:0051495 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0071496 GO:0071840 GO:0080090 GO:0090066 GO:0090702 GO:0098630 GO:0098743 GO:0099120 GO:0110053 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04714 map04136 map04138 map04140 map04150 map04151 map04714
Pfam Domains
Protein Families
WD40

Protein Analysis

318

Amino Acids

35.59

Weight (kDa)

5.77

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 6 - 109 8.5e-06 EIF3I
Beta-prop_WDR3_1st PF25173 7 - 64 2.8e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 7 - 196 1.7e-29 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 7 - 68 4.4e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 7 - 110 2.9e-10 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 8 - 111 1e-12 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 8 - 113 2.2e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 8 - 110 6.7e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 9 - 114 1.2e-12 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 13 - 117 1.7e-07 WDR36/Utp21 first beta-propeller
WDR55 PF24796 36 - 195 2.4e-16 WDR55
Beta-prop_THOC3 PF25174 37 - 308 2.2e-41 THOC3 beta-propeller domain
WD40_Gbeta PF25391 48 - 196 8.6e-13 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 49 - 248 2.7e-19 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 57 - 192 3.1e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 60 - 245 1e-19 Prp19 WD40 domain
WD40 PF00400 70 - 107 1.7e-10 WD domain, G-beta repeat
EIF3I PF24805 74 - 250 2.5e-06 EIF3I
Beta-prop_WDR90_POC16_2nd PF23393 98 - 191 8.7e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 103 - 303 1.5e-18 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 124 - 191 5.6e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 127 - 295 2.8e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 132 - 305 1.1e-16 TEP-1 second beta-propeller
WD40_Gbeta PF25391 168 - 307 2e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 205 - 288 2e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 224 - 309 4.2e-21 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 228 - 310 5.5e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 229 - 310 1.1e-10 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 229 - 302 6.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 231 - 300 1.1e-10 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 232 - 301 1.5e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 247 - 302 1.2e-06 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 251 - 287 1.2e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
AasI GACNNNNNNGTC 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 812
AccI GTMKAC 1 cut(s) 658
AciI CCGC 2 cut(s) 79, 161
AcoI YGGCCR 2 cut(s) 76, 696
AcsI RAATTY 3 cut(s) 312, 454, 601
AfaI GTAC 2 cut(s) 91, 304
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 4 cut(s) 198, 263, 626, 843
AjnI CCWGG 3 cut(s) 150, 331, 943
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 8 cut(s) 39, 222, 329, 365, 528, 622, 830, 938
AluI AGCT 8 cut(s) 39, 222, 329, 365, 528, 622, 830, 938
Alw21I GWGCWC 2 cut(s) 331, 925
Alw44I GTGCAC 1 cut(s) 921
AoxI GGCC 3 cut(s) 66, 76, 696
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 6 cut(s) 81, 155, 158, 362, 382, 539
ApoI RAATTY 3 cut(s) 312, 454, 601
AsiGI ACCGGT 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 474
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 2 cut(s) 415, 947
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 31, 226, 657
AvaII GGWCC 2 cut(s) 415, 947
BaeGI GKGCMC 1 cut(s) 925
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BalI TGGCCA 1 cut(s) 698
BanI GGYRCC 1 cut(s) 812
BanII GRGCYC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 331, 925
BbvI GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BccI CCATC 7 cut(s) 101, 263, 293, 515, 734, 803, 926
BceAI ACGGC 2 cut(s) 48, 342
BciT130I CCWGG 3 cut(s) 152, 333, 945
BciVI GTATCC 1 cut(s) 632
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 574
BfuI GTATCC 1 cut(s) 632
BisI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
BlsI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
Bme1390I CCNGG 4 cut(s) 137, 152, 333, 945
Bme18I GGWCC 2 cut(s) 415, 947
BmgT120I GGNCC 2 cut(s) 415, 947
BmiI GGNNCC 5 cut(s) 292, 416, 477, 814, 860
BmrFI CCNGG 4 cut(s) 137, 152, 333, 945
BmsI GCATC 2 cut(s) 781, 907
BpmI CTGGAG 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 135, 294
BsaWI WCCGGW 2 cut(s) 211, 869
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 281
BseBI CCWGG 3 cut(s) 152, 333, 945
BseDI CCNNGG 2 cut(s) 135, 294
BseGI GGATG 5 cut(s) 93, 285, 304, 526, 645
BseMII CTCAG 1 cut(s) 660
BseNI ACTGG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BsgI GTGCAG 2 cut(s) 368, 381
BshFI GGCC 3 cut(s) 68, 78, 698
BshNI GGYRCC 1 cut(s) 812
BshTI ACCGGT 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 331, 925
BsiSI CCGG 3 cut(s) 137, 212, 870
BslFI GGGAC 4 cut(s) 428, 568, 598, 803
BsmFI GGGAC 4 cut(s) 428, 568, 598, 803
BsnI GGCC 3 cut(s) 68, 78, 698
Bsp1286I GDGCHC 2 cut(s) 331, 925
BspACI CCGC 2 cut(s) 79, 161
BspANI GGCC 3 cut(s) 68, 78, 698
BspCNI CTCAG 1 cut(s) 661
BspLI GGNNCC 5 cut(s) 292, 416, 477, 814, 860
BspMAI CTGCAG 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 812
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 2 cut(s) 135, 294
Bst2UI CCWGG 3 cut(s) 152, 333, 945
Bst4CI ACNGT 9 cut(s) 15, 89, 203, 307, 376, 511, 721, 780, 794
Bst6I CTCTTC 1 cut(s) 712
BstC8I GCNNGC 2 cut(s) 37, 163
BstDEI CTNAG 1 cut(s) 669
BstF5I GGATG 5 cut(s) 93, 285, 304, 526, 645
BstH2I RGCGCY 1 cut(s) 574
BstHHI GCGC 1 cut(s) 573
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstNI CCWGG 3 cut(s) 152, 333, 945
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 4 cut(s) 135, 150, 331, 943
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 925
BstV1I GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 3 cut(s) 68, 78, 698
BtsCI GGATG 5 cut(s) 93, 285, 304, 526, 645
BtsI GCAGTG 3 cut(s) 258, 699, 923
BtsIMutI CAGTG 5 cut(s) 258, 372, 699, 717, 923
Cac8I GCNNGC 2 cut(s) 37, 163
CfoI GCGC 1 cut(s) 573
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 415, 947
CsiI ACCWGGT 1 cut(s) 943
Csp6I GTAC 2 cut(s) 90, 303
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 3 cut(s) 551, 558, 929
CviQI GTAC 2 cut(s) 90, 303
DdeI CTNAG 1 cut(s) 669
DrdI GACNNNNNNGTC 1 cut(s) 719
DseDI GACNNNNNNGTC 1 cut(s) 719
EaeI YGGCCR 2 cut(s) 76, 696
Eam1104I CTCTTC 1 cut(s) 712
EarI CTCTTC 1 cut(s) 712
Ecl136II GAGCTC 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 331
Eco32I GATATC 1 cut(s) 880
Eco47I GGWCC 2 cut(s) 415, 947
Eco53kI GAGCTC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 3 cut(s) 150, 331, 943
EcoRV GATATC 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 331
FaeI CATG 3 cut(s) 554, 561, 932
FaqI GGGAC 4 cut(s) 428, 568, 598, 803
FatI CATG 3 cut(s) 550, 557, 928
FauI CCCGC 1 cut(s) 154
FblI GTMKAC 1 cut(s) 658
Fnu4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FokI GGATG 5 cut(s) 80, 292, 311, 533, 632
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FspBI CTAG 1 cut(s) 764
GlaI GCGC 1 cut(s) 572
GluI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
GsuI CTGGAG 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 574
HaeIII GGCC 3 cut(s) 68, 78, 698
HapII CCGG 3 cut(s) 137, 212, 870
HhaI GCGC 1 cut(s) 573
Hin1II CATG 3 cut(s) 554, 561, 932
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HincII GTYRAC 2 cut(s) 370, 507
HindII GTYRAC 2 cut(s) 370, 507
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 5 cut(s) 107, 147, 227, 391, 839
HpaI GTTAAC 2 cut(s) 370, 507
HpaII CCGG 3 cut(s) 137, 212, 870
HphI GGTGA 3 cut(s) 31, 226, 657
Hpy166II GTNNAC 6 cut(s) 370, 507, 659, 680, 867, 923
Hpy188I TCNGA 4 cut(s) 295, 396, 712, 838
Hpy188III TCNNGA 4 cut(s) 479, 724, 764, 954
Hpy8I GTNNAC 6 cut(s) 370, 507, 659, 680, 867, 923
Hpy99I CGWCG 2 cut(s) 19, 34
HpyAV CCTTC 1 cut(s) 761
HpyCH4III ACNGT 9 cut(s) 15, 89, 203, 307, 376, 511, 721, 780, 794
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 7 cut(s) 158, 362, 385, 462, 542, 923, 928
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 1 cut(s) 669
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 3 cut(s) 554, 561, 932
HspAI GCGC 1 cut(s) 571
KspAI GTTAAC 2 cut(s) 370, 507
LmnI GCTCC 3 cut(s) 334, 525, 935
Lsp1109I GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
LweI GCATC 2 cut(s) 781, 907
MabI ACCWGGT 1 cut(s) 943
MaeI CTAG 1 cut(s) 764
MaeII ACGT 1 cut(s) 243
MboII GAAGA 2 cut(s) 699, 887
MhlI GDGCHC 2 cut(s) 331, 925
MlsI TGGCCA 1 cut(s) 698
MluCI AATT 5 cut(s) 193, 312, 454, 470, 601
MluNI TGGCCA 1 cut(s) 698
MnlI CCTC 9 cut(s) 58, 180, 216, 289, 475, 575, 659, 715, 844
Mox20I TGGCCA 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 698
MseI TTAA 3 cut(s) 192, 369, 506
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 698
MspA1I CMGCKG 3 cut(s) 81, 161, 365
MspI CCGG 3 cut(s) 137, 212, 870
MspR9I CCNGG 4 cut(s) 137, 152, 333, 945
MvaI CCWGG 3 cut(s) 152, 333, 945
MwoI GCNNNNNNNGC 1 cut(s) 577
NciI CCSGG 1 cut(s) 137
NlaIII CATG 3 cut(s) 554, 561, 932
NlaIV GGNNCC 5 cut(s) 292, 416, 477, 814, 860
NspI RCATGY 1 cut(s) 561
OliI CACNNNNGTG 1 cut(s) 377
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 5 cut(s) 107, 147, 227, 391, 839
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
PsiI TTATAA 1 cut(s) 824
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 3 cut(s) 150, 331, 943
PspGI CCWGG 3 cut(s) 150, 331, 943
PspN4I GGNNCC 5 cut(s) 292, 416, 477, 814, 860
PspPI GGNCC 2 cut(s) 415, 947
PstI CTGCAG 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 91, 304
RsaNI GTAC 2 cut(s) 90, 303
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 192, 369, 506
SatI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
Sau96I GGNCC 2 cut(s) 415, 947
ScrFI CCNGG 4 cut(s) 137, 152, 333, 945
SduI GDGCHC 2 cut(s) 331, 925
SexAI ACCWGGT 1 cut(s) 943
SfaNI GCATC 2 cut(s) 781, 907
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 2 cut(s) 415, 947
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 5 cut(s) 193, 312, 454, 470, 601
SsiI CCGC 2 cut(s) 79, 161
SspMI CTAG 1 cut(s) 764
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 4 cut(s) 135, 150, 331, 943
TaaI ACNGT 9 cut(s) 15, 89, 203, 307, 376, 511, 721, 780, 794
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 178
TasI AATT 5 cut(s) 193, 312, 454, 470, 601
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 5 cut(s) 107, 147, 227, 391, 839
Tru1I TTAA 3 cut(s) 192, 369, 506
Tru9I TTAA 3 cut(s) 192, 369, 506
TscAI CASTG 5 cut(s) 258, 379, 706, 724, 930
TseI GCWGC 6 cut(s) 81, 155, 158, 362, 382, 539
TspDTI ATGAA 2 cut(s) 366, 605
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 5 cut(s) 258, 379, 706, 724, 930
VneI GTGCAC 1 cut(s) 921
VpaK11BI GGWCC 2 cut(s) 415, 947
XapI RAATTY 3 cut(s) 312, 454, 601
XbaI TCTAGA 1 cut(s) 763
XceI RCATGY 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.