RchiOBHm_Chr3g0481011

Protein LST8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007389
Physical Location & Seq
Forward (+)
27042917 .. 27046704
3788 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44600

Sequence Viewer

Length: 954 bp
ATGAGCCAACAACCGTCGGTGATACTTGCGACGGCGAGCTATGACCACACTATTCGCTTCTGGGAGGCCAAAAGTGGCCGCTGCTACCGTACCATCCAATACCCTGATTCACAAGTAAATAGGCTTGAGATAACTCCGGATAAACGATTCCTGGCTGCAGCGGGCAACCCTCATATTCGATTGTTTGATGTTAATTCAAACAGTCCTCAACCGGTGATGAGCTATGATTCACACACTAATAACGTAATGGCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCCGAGGACGGTACAGTAAAAATTTGGGATTTGAGAGCTCCAGGTTGCCAAAGGGAATATGAAAGCCGTGCAGCTGTTAACACTGTTGTGCTGCACCCGAATCAGACTGAACTAATATCTGGGGACCAAAATGGCAACATTCGTGTTTGGGATTTGACAGCGAATTCTTGCAGTTGTGAATTGGTTCCAGAGGTGGATACTGCTGTAAGGTCGTTAACAGTTATGTGGGATGGGAGCTTGGTTGTTGCTGCAAATAATCATGGGACATGCTATGTTTGGCGCTTGTTGCGAGGGACACAGACTATGACAAATTTTGAGCCACTTCATAAGCTCCAAGCACACAAGGGATACATCCTCAAATGTCTACTTTCACCTGAGTTCTGTGAACCCCACAGATATTTGGCCACTGCCTCTTCTGACCACACTGTCAAGATATGGAATGTTGATGGTTTCACATTAGAGAAAACTCTAGAAGGGCATCAACGGTGGGTTTGGGACTGTGTTTTTTCCGTAGATGGTGCCTATCTTATAACAGCTTCCTCTGATACAACAGCAAGGCTTTGGAACCTGTCCACCGGAGAAGATATCAGGGTGTATCAGGGGCATCACAAAGCAACCATTTGTTGTGCACTGCATGATGGAGCTGAACCTGGTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006935 GO:0006950 GO:0007154 GO:0007165 GO:0007190 GO:0008064 GO:0008150 GO:0009267 GO:0009605 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0010638 GO:0016043 GO:0019220 GO:0019222 GO:0023052 GO:0030100 GO:0030587 GO:0030832 GO:0030833 GO:0030838 GO:0031152 GO:0031252 GO:0031323 GO:0031325 GO:0031334 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0031932 GO:0032147 GO:0032148 GO:0032268 GO:0032270 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040011 GO:0042221 GO:0042325 GO:0042327 GO:0042330 GO:0042594 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043327 GO:0043519 GO:0043520 GO:0043549 GO:0044087 GO:0044089 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045806 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0050764 GO:0050765 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051493 GO:0051495 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0071496 GO:0071840 GO:0080090 GO:0090066 GO:0090702 GO:0098630 GO:0098743 GO:0099120 GO:0110053 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04714 map04136 map04138 map04140 map04150 map04151 map04714
Pfam Domains
Protein Families
WD40

Protein Analysis

317

Amino Acids

35.51

Weight (kDa)

5.88

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 6 - 110 3.6e-06 EIF3I
Beta-prop_WDR3_1st PF25173 7 - 64 2.8e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 7 - 196 1.8e-29 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 7 - 66 7e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 7 - 110 2.3e-10 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 8 - 113 2.6e-14 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 8 - 111 9.2e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 8 - 110 6.2e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 9 - 114 1.1e-12 WDHD1 first WD40 domain
Beta-prop_EML PF23409 10 - 109 1e-05 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 13 - 114 2e-07 WDR36/Utp21 first beta-propeller
WDR55 PF24796 36 - 195 2.6e-16 WDR55
Beta-prop_THOC3 PF25174 37 - 307 5.7e-42 THOC3 beta-propeller domain
WD40_Gbeta PF25391 48 - 196 8.8e-13 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 49 - 246 1.4e-19 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 58 - 192 3.6e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 60 - 244 4.2e-20 Prp19 WD40 domain
WD40 PF00400 70 - 107 1.7e-10 WD domain, G-beta repeat
Beta-prop_WDR90_POC16_2nd PF23393 97 - 191 8.2e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 102 - 302 7.5e-19 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 119 - 287 2.4e-25 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 124 - 191 5.2e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 127 - 294 7.9e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 132 - 304 6.6e-18 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 165 - 306 2.2e-10 WDHD1 first WD40 domain
WD40_Gbeta PF25391 167 - 306 5.7e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 205 - 287 2.1e-15 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 222 - 308 2e-21 WDR5 beta-propeller domain
WD40_Prp19 PF24814 228 - 307 8.1e-11 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 230 - 299 2.9e-10 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 231 - 300 1.6e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 245 - 301 1.1e-06 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 250 - 286 2.3e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
AasI GACNNNNNNGTC 1 cut(s) 716
AccB1I GGYRCC 1 cut(s) 809
AccI GTMKAC 1 cut(s) 655
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 2 cut(s) 79, 161
AcoI YGGCCR 2 cut(s) 76, 693
AcsI RAATTY 3 cut(s) 312, 454, 601
AfaI GTAC 2 cut(s) 91, 304
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 2 cut(s) 198, 263
AjnI CCWGG 3 cut(s) 150, 331, 940
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 8 cut(s) 39, 222, 329, 365, 528, 622, 827, 935
AluI AGCT 8 cut(s) 39, 222, 329, 365, 528, 622, 827, 935
Alw21I GWGCWC 2 cut(s) 331, 922
Alw44I GTGCAC 1 cut(s) 918
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 3 cut(s) 66, 76, 693
ApaLI GTGCAC 1 cut(s) 918
ApeKI GCWGC 6 cut(s) 81, 155, 158, 362, 382, 539
ApoI RAATTY 3 cut(s) 312, 454, 601
AsiGI ACCGGT 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 474
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 2 cut(s) 415, 944
AsuHPI GGTGA 3 cut(s) 31, 226, 654
AvaII GGWCC 2 cut(s) 415, 944
BaeGI GKGCMC 1 cut(s) 922
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BalI TGGCCA 1 cut(s) 695
BanI GGYRCC 1 cut(s) 809
BanII GRGCYC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 331, 922
BbvI GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BccI CCATC 6 cut(s) 101, 263, 515, 731, 800, 923
BceAI ACGGC 2 cut(s) 48, 342
BciT130I CCWGG 3 cut(s) 152, 333, 942
BciVI GTATCC 2 cut(s) 481, 632
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 574
BfuI GTATCC 2 cut(s) 481, 632
BisI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
BlsI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
Bme1390I CCNGG 3 cut(s) 152, 333, 942
Bme18I GGWCC 2 cut(s) 415, 944
BmgT120I GGNCC 2 cut(s) 415, 944
BmiI GGNNCC 5 cut(s) 292, 416, 477, 811, 857
BmrFI CCNGG 3 cut(s) 152, 333, 942
BmsI GCATC 2 cut(s) 778, 904
BpmI CTGGAG 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 3 cut(s) 136, 211, 866
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 281
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 3 cut(s) 152, 333, 942
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 4 cut(s) 93, 285, 526, 642
BseMII CTCAG 1 cut(s) 657
BseNI ACTGG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BsgI GTGCAG 2 cut(s) 368, 381
BshFI GGCC 3 cut(s) 68, 78, 695
BshNI GGYRCC 1 cut(s) 809
BshTI ACCGGT 1 cut(s) 211
BsiHKAI GWGCWC 2 cut(s) 331, 922
BsiSI CCGG 3 cut(s) 137, 212, 867
BslFI GGGAC 4 cut(s) 428, 568, 598, 800
BsmFI GGGAC 4 cut(s) 428, 568, 598, 800
BsnI GGCC 3 cut(s) 68, 78, 695
Bsp1286I GDGCHC 2 cut(s) 331, 922
Bsp13I TCCGGA 1 cut(s) 136
BspACI CCGC 2 cut(s) 79, 161
BspANI GGCC 3 cut(s) 68, 78, 695
BspCNI CTCAG 1 cut(s) 658
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 5 cut(s) 292, 416, 477, 811, 857
BspMAI CTGCAG 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 809
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 294
Bst2UI CCWGG 3 cut(s) 152, 333, 942
Bst6I CTCTTC 1 cut(s) 709
BstC8I GCNNGC 2 cut(s) 37, 163
BstDEI CTNAG 1 cut(s) 666
BstF5I GGATG 4 cut(s) 93, 285, 526, 642
BstH2I RGCGCY 1 cut(s) 574
BstHHI GCGC 1 cut(s) 573
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstNI CCWGG 3 cut(s) 152, 333, 942
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 3 cut(s) 150, 331, 940
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 922
BstV1I GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BsuI GTATCC 2 cut(s) 481, 632
BsuRI GGCC 3 cut(s) 68, 78, 695
BtsCI GGATG 4 cut(s) 93, 285, 526, 642
BtsI GCAGTG 3 cut(s) 258, 696, 920
BtsIMutI CAGTG 5 cut(s) 258, 372, 696, 714, 920
Cac8I GCNNGC 2 cut(s) 37, 163
CfoI GCGC 1 cut(s) 573
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 415, 944
CsiI ACCWGGT 1 cut(s) 940
Csp6I GTAC 2 cut(s) 90, 303
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 3 cut(s) 551, 558, 926
CviQI GTAC 2 cut(s) 90, 303
DdeI CTNAG 1 cut(s) 666
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 2 cut(s) 76, 693
Eam1104I CTCTTC 1 cut(s) 709
EarI CTCTTC 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 331
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 2 cut(s) 415, 944
Eco53kI GAGCTC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 3 cut(s) 150, 331, 940
EcoRV GATATC 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 331
FaeI CATG 3 cut(s) 554, 561, 929
FaqI GGGAC 4 cut(s) 428, 568, 598, 800
FatI CATG 3 cut(s) 550, 557, 925
FauI CCCGC 1 cut(s) 154
FblI GTMKAC 1 cut(s) 655
Fnu4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FokI GGATG 4 cut(s) 80, 292, 533, 629
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FspBI CTAG 1 cut(s) 761
GlaI GCGC 1 cut(s) 572
GluI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
GsuI CTGGAG 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 574
HaeIII GGCC 3 cut(s) 68, 78, 695
HapII CCGG 3 cut(s) 137, 212, 867
HhaI GCGC 1 cut(s) 573
Hin1II CATG 3 cut(s) 554, 561, 929
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HincII GTYRAC 2 cut(s) 370, 507
HindII GTYRAC 2 cut(s) 370, 507
HinfI GANTC 4 cut(s) 107, 147, 227, 391
HpaI GTTAAC 2 cut(s) 370, 507
HpaII CCGG 3 cut(s) 137, 212, 867
HphI GGTGA 3 cut(s) 31, 226, 654
Hpy166II GTNNAC 6 cut(s) 370, 507, 656, 677, 864, 920
Hpy188I TCNGA 4 cut(s) 295, 396, 709, 835
Hpy188III TCNNGA 5 cut(s) 137, 479, 721, 761, 951
Hpy8I GTNNAC 6 cut(s) 370, 507, 656, 677, 864, 920
Hpy99I CGWCG 2 cut(s) 19, 34
HpyAV CCTTC 1 cut(s) 758
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 7 cut(s) 158, 362, 385, 462, 542, 920, 925
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 1 cut(s) 666
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 3 cut(s) 554, 561, 929
HspAI GCGC 1 cut(s) 571
Kpn2I TCCGGA 1 cut(s) 136
KspAI GTTAAC 2 cut(s) 370, 507
LmnI GCTCC 4 cut(s) 334, 525, 627, 932
Lsp1109I GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
LweI GCATC 2 cut(s) 778, 904
MabI ACCWGGT 1 cut(s) 940
MaeI CTAG 1 cut(s) 761
MaeII ACGT 1 cut(s) 243
MboII GAAGA 2 cut(s) 696, 884
MhlI GDGCHC 2 cut(s) 331, 922
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 5 cut(s) 193, 312, 454, 470, 601
MluNI TGGCCA 1 cut(s) 695
MnlI CCTC 9 cut(s) 58, 180, 216, 289, 475, 575, 656, 712, 841
Mox20I TGGCCA 1 cut(s) 695
MroI TCCGGA 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 695
MseI TTAA 3 cut(s) 192, 369, 506
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 695
MspA1I CMGCKG 3 cut(s) 81, 161, 365
MspI CCGG 3 cut(s) 137, 212, 867
MspR9I CCNGG 3 cut(s) 152, 333, 942
MvaI CCWGG 3 cut(s) 152, 333, 942
MwoI GCNNNNNNNGC 1 cut(s) 577
NlaIII CATG 3 cut(s) 554, 561, 929
NlaIV GGNNCC 5 cut(s) 292, 416, 477, 811, 857
NspI RCATGY 1 cut(s) 561
OliI CACNNNNGTG 1 cut(s) 377
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 4 cut(s) 107, 147, 227, 391
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
PsiI TTATAA 1 cut(s) 821
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 3 cut(s) 150, 331, 940
PspGI CCWGG 3 cut(s) 150, 331, 940
PspN4I GGNNCC 5 cut(s) 292, 416, 477, 811, 857
PspPI GGNCC 2 cut(s) 415, 944
PstI CTGCAG 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 91, 304
RsaNI GTAC 2 cut(s) 90, 303
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 192, 369, 506
SatI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
Sau96I GGNCC 2 cut(s) 415, 944
ScrFI CCNGG 3 cut(s) 152, 333, 942
SduI GDGCHC 2 cut(s) 331, 922
SexAI ACCWGGT 1 cut(s) 940
SfaNI GCATC 2 cut(s) 778, 904
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 2 cut(s) 415, 944
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 5 cut(s) 193, 312, 454, 470, 601
SsiI CCGC 2 cut(s) 79, 161
SspMI CTAG 1 cut(s) 761
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 3 cut(s) 150, 331, 940
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 178
TasI AATT 5 cut(s) 193, 312, 454, 470, 601
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 4 cut(s) 107, 147, 227, 391
Tru1I TTAA 3 cut(s) 192, 369, 506
Tru9I TTAA 3 cut(s) 192, 369, 506
TscAI CASTG 5 cut(s) 258, 379, 703, 721, 927
TseI GCWGC 6 cut(s) 81, 155, 158, 362, 382, 539
TspDTI ATGAA 2 cut(s) 366, 605
TspGWI ACGGA 1 cut(s) 790
TspRI CASTG 5 cut(s) 258, 379, 703, 721, 927
VneI GTGCAC 1 cut(s) 918
VpaK11BI GGWCC 2 cut(s) 415, 944
XapI RAATTY 3 cut(s) 312, 454, 601
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 655
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.