MD14G1066600.v1.1

Protein LST8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007389
Physical Location & Seq
Forward (+)
7256884 .. 7262534
5651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1066600.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGATTAGGTATTACAATTTCAAACCCAGTTTTTCTGAAGAGGAGACTAAGATGATCCAACAACCATCGGTGATCCTTGCAACTGCTAGCTATGATCACACTATTCGGTTTTGGGAGGCCAAAAGTGGCCGCTGCTACCGTACCATCCAGTACCCTGATTCACAAGTTAATAAGCTCGAGATAACTCCAGATAAACGCTTCCTGGCTGCAGCCGGAAATCCTCACATTCGATTATTTGATGTTAATACAAATAGCCCTCAACCGGTGATGAGCTATGAGTCACATAGTAATAATGTAATGGCTGTAGGGTTTCAATGCGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGTACAGTAAAAATTTGGGATCTGAGAGCTCCAGGTTGTCAAAGGGAATATGAAAGTCGTGCAGCTGTTAACACTGTTGTGCTTCACCCGAACCAGACTGAACTAATATCTGGGGACCAAAATGGCAATATTCGTGTTTGGGATTTGACAGCAAATTTGTGCAGCTGTGAATTGGTTCCAGAGGTGGACACGGCTGTAAGGTCATTAACAGTTATGTGGGATGGGAGCTTGGTTGTTGCAGCAAATAATCATGGGACATGCTACGTTTGGCGCTTGTTGCATGGAAACCAGACGATGTCAAATTTTGAGCCACTTCATAAGCTGCAAGCACACAAGGGATACATTCTTAAATGTCTTCTTTCACCTGAATTCTGTGAACCCCACAGATACTTGGCAACCGCCTCTTCGGACCACACTGTCAAGATATGGAATGTAGATGGTTTCACATTAGAGAAAACTTTAGTAGGACATCAACGGTGGGTGTGGGACTGTGTTTTCTCAGCGGACGGTTCCTATCTTATAACAGCTTCCTCTGATACAACAGCGAGACTTTGGTCCATGTCCACCGGTGAAGATATCAGGGTGTATCAGGGGAATCACAAAGCAACAATTTGTTGTGCACTCCATGATGGAGCCGAACCTTCTGCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006935 GO:0006950 GO:0007154 GO:0007165 GO:0007190 GO:0008064 GO:0008150 GO:0009267 GO:0009605 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0010638 GO:0016043 GO:0019220 GO:0019222 GO:0023052 GO:0030100 GO:0030587 GO:0030832 GO:0030833 GO:0030838 GO:0031152 GO:0031252 GO:0031323 GO:0031325 GO:0031334 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0031932 GO:0032147 GO:0032148 GO:0032268 GO:0032270 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040011 GO:0042221 GO:0042325 GO:0042327 GO:0042330 GO:0042594 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043327 GO:0043519 GO:0043520 GO:0043549 GO:0044087 GO:0044089 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045806 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0050764 GO:0050765 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051493 GO:0051495 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0071496 GO:0071840 GO:0080090 GO:0090066 GO:0090702 GO:0098630 GO:0098743 GO:0099120 GO:0110053 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04714 map04136 map04138 map04140 map04150 map04151 map04714
Pfam Domains
Protein Families
WD40

Protein Analysis

335

Amino Acids

37.61

Weight (kDa)

5.89

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 19 - 131 1.2e-13 WDHD1 first WD40 domain
EIF3I PF24805 20 - 128 2e-06 EIF3I
WD40_MABP1-WDR62_2nd PF24782 22 - 125 9.2e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 23 - 81 1.1e-07 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 23 - 127 3.6e-10 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 23 - 128 2e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 23 - 212 2.3e-29 WDR5 beta-propeller domain
WD40_Prp19 PF24814 24 - 191 9.9e-21 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 25 - 85 2.1e-08 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 25 - 127 4.2e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 26 - 126 4.6e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 30 - 131 7.4e-08 WDR36/Utp21 first beta-propeller
WDR55 PF24796 53 - 211 6.5e-16 WDR55
Beta-prop_THOC3 PF25174 54 - 323 3.5e-41 THOC3 beta-propeller domain
WD40_Gbeta PF25391 64 - 214 3.4e-13 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 66 - 264 5.8e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 75 - 209 7.3e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 78 - 261 1.9e-19 Prp19 WD40 domain
WD40 PF00400 87 - 124 1.9e-10 WD domain, G-beta repeat
Beta-prop_WDR90_POC16_2nd PF23393 114 - 209 3.1e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 120 - 311 4.1e-18 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 141 - 208 2e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 144 - 311 8.3e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 149 - 323 5.4e-16 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 182 - 323 1.2e-09 WDHD1 first WD40 domain
WD40_Gbeta PF25391 185 - 323 5.3e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 222 - 326 1.2e-10 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 222 - 303 3e-16 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 227 - 303 7.4e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR5 PF25175 239 - 325 9.8e-20 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 247 - 317 7e-10 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 248 - 319 5e-07 EIF3I
WD40 PF00400 267 - 303 4.9e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 872
AasI GACNNNNNNGTC 1 cut(s) 767
AciI CCGC 3 cut(s) 130, 750, 854
AclWI GGATC 3 cut(s) 49, 67, 378
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 4 cut(s) 363, 505, 652, 719
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 3 cut(s) 142, 152, 355
AfiI CCNNNNNNNGG 1 cut(s) 262
AgeI ACCGGT 2 cut(s) 262, 917
AgsI TTSAA 2 cut(s) 22, 314
AjnI CCWGG 2 cut(s) 201, 382
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 9 cut(s) 90, 175, 273, 380, 416, 516, 579, 673, 878
AluI AGCT 9 cut(s) 90, 175, 273, 380, 416, 516, 579, 673, 878
Alw21I GWGCWC 2 cut(s) 382, 973
Alw26I GTCTC 2 cut(s) 38, 892
Alw44I GTGCAC 1 cut(s) 969
AlwI GGATC 3 cut(s) 49, 67, 378
AlwNI CAGNNNCTG 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 2 cut(s) 117, 127
ApaLI GTGCAC 1 cut(s) 969
ApeKI GCWGC 7 cut(s) 132, 206, 209, 413, 513, 590, 673
ApoI RAATTY 4 cut(s) 363, 505, 652, 719
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
AsiGI ACCGGT 2 cut(s) 262, 917
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 1 cut(s) 624
AspS9I GGNCC 3 cut(s) 466, 760, 906
AsuHPI GGTGA 5 cut(s) 82, 277, 428, 705, 932
AsuNHI GCTAGC 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 3 cut(s) 466, 760, 906
BaeGI GKGCMC 1 cut(s) 973
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 2 cut(s) 382, 973
BbvI GCAGC 7 cut(s) 119, 193, 221, 425, 525, 602, 660
BccI CCATC 7 cut(s) 73, 152, 314, 344, 566, 782, 974
BceAI ACGGC 1 cut(s) 558
BcgI CGANNNNNNTGC 1 cut(s) 977
BciT130I CCWGG 2 cut(s) 203, 384
BciVI GTATCC 1 cut(s) 683
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 38, 892
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 2 cut(s) 207, 303
BfoI RGCGCY 1 cut(s) 625
BfuI GTATCC 1 cut(s) 683
BisI GCNGC 8 cut(s) 130, 133, 207, 210, 414, 514, 591, 674
BlsI GCNGC 8 cut(s) 131, 134, 208, 211, 415, 515, 592, 675
Bme1390I CCNGG 2 cut(s) 203, 384
Bme18I GGWCC 3 cut(s) 466, 760, 906
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 3 cut(s) 466, 760, 906
BmiI GGNNCC 4 cut(s) 467, 528, 862, 985
BmrFI CCNGG 2 cut(s) 203, 384
BmrI ACTGGG 1 cut(s) 21
BmtI GCTAGC 1 cut(s) 90
BmuI ACTGGG 1 cut(s) 21
BoxI GACNNNNGTC 1 cut(s) 904
BpiI GAAGAC 1 cut(s) 698
BpmI CTGGAG 2 cut(s) 171, 366
BsaWI WCCGGW 2 cut(s) 262, 917
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse118I RCCGGY 2 cut(s) 262, 917
Bse1I ACTGG 3 cut(s) 27, 148, 332
BseBI CCWGG 2 cut(s) 203, 384
BseGI GGATG 5 cut(s) 144, 336, 355, 577, 998
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMII CTCAG 3 cut(s) 336, 365, 864
BseNI ACTGG 3 cut(s) 27, 148, 332
BseRI GAGGAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 973
BseXI GCAGC 7 cut(s) 119, 193, 221, 425, 525, 602, 660
BsgI GTGCAG 2 cut(s) 432, 532
BshFI GGCC 2 cut(s) 119, 129
BshTI ACCGGT 2 cut(s) 262, 917
BsiHKAI GWGCWC 2 cut(s) 382, 973
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 3 cut(s) 213, 263, 918
BslFI GGGAC 3 cut(s) 479, 619, 851
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 2 cut(s) 38, 892
BsmFI GGGAC 3 cut(s) 479, 619, 851
BsnI GGCC 2 cut(s) 119, 129
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 382, 973
Bsp143I GATC 4 cut(s) 54, 72, 94, 370
BspACI CCGC 3 cut(s) 130, 750, 854
BspANI GGCC 2 cut(s) 119, 129
BspCNI CTCAG 3 cut(s) 337, 366, 863
BspLI GGNNCC 4 cut(s) 467, 528, 862, 985
BspMAI CTGCAG 1 cut(s) 211
BspOI GCTAGC 1 cut(s) 90
BspPI GGATC 3 cut(s) 49, 67, 378
BsrFI RCCGGY 2 cut(s) 262, 917
BsrI ACTGG 3 cut(s) 27, 148, 332
BssAI RCCGGY 2 cut(s) 262, 917
BssMI GATC 4 cut(s) 54, 72, 94, 370
Bst2UI CCWGG 2 cut(s) 203, 384
Bst4CI ACNGT 8 cut(s) 140, 358, 427, 562, 769, 828, 842, 860
Bst6I CTCTTC 2 cut(s) 33, 760
BstC8I GCNNGC 2 cut(s) 88, 678
BstDEI CTNAG 4 cut(s) 48, 345, 374, 850
BstF5I GGATG 5 cut(s) 144, 336, 355, 577, 998
BstH2I RGCGCY 1 cut(s) 625
BstHHI GCGC 1 cut(s) 624
BstKTI GATC 4 cut(s) 57, 75, 97, 373
BstMAI GTCTC 2 cut(s) 38, 892
BstMBI GATC 4 cut(s) 54, 72, 94, 370
BstMWI GCNNNNNNNGC 1 cut(s) 628
BstNI CCWGG 2 cut(s) 203, 384
BstNSI RCATGY 1 cut(s) 612
BstPAI GACNNNNGTC 1 cut(s) 904
BstSCI CCNGG 2 cut(s) 201, 382
BstSFI CTRYAG 2 cut(s) 207, 303
BstSLI GKGCMC 1 cut(s) 973
BstV1I GCAGC 7 cut(s) 119, 193, 221, 425, 525, 602, 660
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 2 cut(s) 119, 129
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 5 cut(s) 144, 336, 355, 577, 998
BtsIMutI CAGTG 2 cut(s) 423, 765
Cac8I GCNNGC 2 cut(s) 88, 678
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 1 cut(s) 624
Cfr10I RCCGGY 2 cut(s) 262, 917
Cfr13I GGNCC 3 cut(s) 466, 760, 906
Csp6I GTAC 3 cut(s) 141, 151, 354
CspAI ACCGGT 2 cut(s) 262, 917
CviAII CATG 5 cut(s) 602, 609, 632, 910, 977
CviQI GTAC 3 cut(s) 141, 151, 354
DdeI CTNAG 4 cut(s) 48, 345, 374, 850
DpnI GATC 4 cut(s) 56, 74, 96, 372
DpnII GATC 4 cut(s) 54, 72, 94, 370
DrdI GACNNNNNNGTC 1 cut(s) 767
DseDI GACNNNNNNGTC 1 cut(s) 767
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 33, 760
EarI CTCTTC 2 cut(s) 33, 760
Ecl136II GAGCTC 1 cut(s) 380
Eco24I GRGCYC 1 cut(s) 382
Eco32I GATATC 1 cut(s) 928
Eco47I GGWCC 3 cut(s) 466, 760, 906
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 719
EcoRII CCWGG 2 cut(s) 201, 382
EcoRV GATATC 1 cut(s) 928
EcoT38I GRGCYC 1 cut(s) 382
FaeI CATG 5 cut(s) 605, 612, 635, 913, 980
FaqI GGGAC 3 cut(s) 479, 619, 851
FatI CATG 5 cut(s) 601, 608, 631, 909, 976
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 8 cut(s) 130, 133, 207, 210, 414, 514, 591, 674
FokI GGATG 5 cut(s) 131, 343, 362, 584, 985
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 8 cut(s) 130, 133, 207, 210, 414, 514, 591, 674
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 623
GluI GCNGC 8 cut(s) 130, 133, 207, 210, 414, 514, 591, 674
GsuI CTGGAG 2 cut(s) 171, 366
HaeII RGCGCY 1 cut(s) 625
HaeIII GGCC 2 cut(s) 119, 129
HapII CCGG 3 cut(s) 213, 263, 918
HhaI GCGC 1 cut(s) 624
Hin1II CATG 5 cut(s) 605, 612, 635, 913, 980
Hin6I GCGC 1 cut(s) 622
HinP1I GCGC 1 cut(s) 622
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 3 cut(s) 158, 278, 946
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 3 cut(s) 213, 263, 918
HphI GGTGA 5 cut(s) 82, 277, 428, 705, 932
Hpy166II GTNNAC 5 cut(s) 421, 538, 728, 915, 971
Hpy188I TCNGA 5 cut(s) 37, 346, 375, 760, 886
Hpy188III TCNNGA 5 cut(s) 178, 188, 530, 772, 1002
Hpy8I GTNNAC 5 cut(s) 421, 538, 728, 915, 971
HpyAV CCTTC 1 cut(s) 1002
HpyCH4III ACNGT 8 cut(s) 140, 358, 427, 562, 769, 828, 842, 860
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 9 cut(s) 80, 209, 413, 513, 590, 631, 676, 971, 998
HpyF10VI GCNNNNNNNGC 1 cut(s) 628
HpyF3I CTNAG 4 cut(s) 48, 345, 374, 850
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 5 cut(s) 605, 612, 635, 913, 980
HspAI GCGC 1 cut(s) 622
Ksp22I TGATCA 1 cut(s) 94
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 4 cut(s) 54, 72, 94, 370
LmnI GCTCC 3 cut(s) 385, 576, 983
Lsp1109I GCAGC 7 cut(s) 119, 193, 221, 425, 525, 602, 660
MaeI CTAG 1 cut(s) 87
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 1 cut(s) 279
MalI GATC 4 cut(s) 56, 74, 96, 372
MboI GATC 4 cut(s) 54, 72, 94, 370
MboII GAAGA 4 cut(s) 50, 698, 747, 935
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 2 cut(s) 382, 973
MluCI AATT 7 cut(s) 16, 363, 505, 521, 652, 719, 960
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 8 cut(s) 34, 109, 231, 267, 340, 526, 763, 892
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 5 cut(s) 168, 243, 420, 557, 699
MslI CAYNNNNRTG 1 cut(s) 428
MspA1I CMGCKG 4 cut(s) 132, 416, 516, 854
MspI CCGG 3 cut(s) 213, 263, 918
MspR9I CCNGG 2 cut(s) 203, 384
MvaI CCWGG 2 cut(s) 203, 384
MwoI GCNNNNNNNGC 1 cut(s) 628
NdeII GATC 4 cut(s) 54, 72, 94, 370
NheI GCTAGC 1 cut(s) 86
NlaIII CATG 5 cut(s) 605, 612, 635, 913, 980
NlaIV GGNNCC 4 cut(s) 467, 528, 862, 985
NmuCI GTSAC 1 cut(s) 279
NspI RCATGY 1 cut(s) 612
OliI CACNNNNGTG 1 cut(s) 428
PaeR7I CTCGAG 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 2 cut(s) 158, 946
PflFI GACNNNGTC 1 cut(s) 646
PinAI ACCGGT 2 cut(s) 262, 917
PkrI GCNGC 8 cut(s) 131, 134, 208, 211, 415, 515, 592, 675
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PshAI GACNNNNGTC 1 cut(s) 904
PsiI TTATAA 1 cut(s) 872
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 2 cut(s) 201, 382
PspGI CCWGG 2 cut(s) 201, 382
PspN4I GGNNCC 4 cut(s) 467, 528, 862, 985
PspPI GGNCC 3 cut(s) 466, 760, 906
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 370
PsyI GACNNNGTC 1 cut(s) 646
PvuII CAGCTG 2 cut(s) 416, 516
RsaI GTAC 3 cut(s) 142, 152, 355
RsaNI GTAC 3 cut(s) 141, 151, 354
RseI CAYNNNNRTG 1 cut(s) 428
SacI GAGCTC 1 cut(s) 382
SaqAI TTAA 5 cut(s) 168, 243, 420, 557, 699
SatI GCNGC 8 cut(s) 130, 133, 207, 210, 414, 514, 591, 674
Sau3AI GATC 4 cut(s) 54, 72, 94, 370
Sau96I GGNCC 3 cut(s) 466, 760, 906
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 203, 384
SduI GDGCHC 2 cut(s) 382, 973
SfcI CTRYAG 2 cut(s) 207, 303
Sfr274I CTCGAG 1 cut(s) 176
SgrAI CRCCGGYG 1 cut(s) 917
SinI GGWCC 3 cut(s) 466, 760, 906
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 7 cut(s) 16, 363, 505, 521, 652, 719, 960
SsiI CCGC 3 cut(s) 130, 750, 854
SspI AATATT 1 cut(s) 481
SspMI CTAG 1 cut(s) 87
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 2 cut(s) 201, 382
TaaI ACNGT 8 cut(s) 140, 358, 427, 562, 769, 828, 842, 860
TaiI ACGT 1 cut(s) 618
TaqI TCGA 2 cut(s) 177, 229
TasI AATT 7 cut(s) 16, 363, 505, 521, 652, 719, 960
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 2 cut(s) 158, 946
Tru1I TTAA 5 cut(s) 168, 243, 420, 557, 699
Tru9I TTAA 5 cut(s) 168, 243, 420, 557, 699
TscAI CASTG 2 cut(s) 430, 772
TseFI GTSAC 1 cut(s) 279
TseI GCWGC 7 cut(s) 132, 206, 209, 413, 513, 590, 673
Tsp45I GTSAC 1 cut(s) 279
TspDTI ATGAA 2 cut(s) 417, 656
TspRI CASTG 2 cut(s) 430, 772
Tth111I GACNNNGTC 1 cut(s) 646
VneI GTGCAC 1 cut(s) 969
VpaK11BI GGWCC 3 cut(s) 466, 760, 906
XapI RAATTY 4 cut(s) 363, 505, 652, 719
XceI RCATGY 1 cut(s) 612
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.