Rh3AG239900

Protein LST8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007389
Physical Location & Seq
Forward (+)
25090642 .. 25094424
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG239900.1

Sequence Viewer

Length: 840 bp
ATGAGCCAACAACCGTCGGTGATACTTGCGACGGCGAGCTATGACCACACTATTCGCTTCTGGGAGGCCAAAAGTGGCCGCTGCTACCGTACCATCCAATACCCTGATTCACAAGTAAATAGGCTTGAGATAACTCCGGATAAACGATTCCTGGCTGCAGCGGGCAACCCTCATATTCGATTGTTTGATGTTAATTCAAACAGTCCTCAACCGGTGATGAGCTATGATTCACACACTAATAACGTAATGGCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCCGAGGACGGTACAGTAAAAATTTGGGATTTGAGAGCTCCAGGTTGCCAAAGGGAATATGAAAGCCGTGCAGCTGTTAACACTGTTGTGCTGCACCCGAATCAGACTGAACTAATATCTGGGGACCAAAATGGCAACATTCGTGTTTGGGATTTGACAGCGAATTCTTGCAGTTGTGAATTGGTTCCAGAGGTGGATACTGCTGTAAGGTCGTTAACAGTTATGTGGGATGGGAGCTTGGTTGTTGCTGCAAATAATCATGGGACATGCTATGTTTGGCGCTTGTTGCGAGGGACACAGACTATGACAAATTTTGAGCCACTTCATAAGCTCCAAGCACACAAGGGATACATCCTCAAATGTCTACTTTCACCTGAGTTCTGTGAACCCCACAGATATTTGGCCACTGCCTCTTCTGACCACACTGTCAAGATATGGAATGTTGATGGTTTCACATTAGAGAAAACTCTAGAAGGTATCTATCTTATACAATTCTCCCAAGATTCTAATATGTACTTTACAAATAACTCAGTAAACCTGATGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006935 GO:0006950 GO:0007154 GO:0007165 GO:0007190 GO:0008064 GO:0008150 GO:0009267 GO:0009605 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0010638 GO:0016043 GO:0019220 GO:0019222 GO:0023052 GO:0030100 GO:0030587 GO:0030832 GO:0030833 GO:0030838 GO:0031152 GO:0031252 GO:0031323 GO:0031325 GO:0031334 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0031932 GO:0032147 GO:0032148 GO:0032268 GO:0032270 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032879 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040011 GO:0042221 GO:0042325 GO:0042327 GO:0042330 GO:0042594 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043327 GO:0043519 GO:0043520 GO:0043549 GO:0044087 GO:0044089 GO:0044093 GO:0044424 GO:0044444 GO:0044464 GO:0044764 GO:0045806 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048856 GO:0050764 GO:0050765 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051493 GO:0051495 GO:0051703 GO:0051704 GO:0051716 GO:0060255 GO:0060627 GO:0065007 GO:0065008 GO:0065009 GO:0071496 GO:0071840 GO:0080090 GO:0090066 GO:0090702 GO:0098630 GO:0098743 GO:0099120 GO:0110053 GO:1902903 GO:1902905
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04714 map04136 map04138 map04140 map04150 map04151 map04714
Pfam Domains
Protein Families
WD40

Protein Analysis

279

Amino Acids

31.51

Weight (kDa)

5.83

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 6 - 110 2.8e-06 EIF3I
Beta-prop_WDR3_1st PF25173 7 - 55 1.5e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 7 - 67 4.2e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 7 - 110 1.7e-10 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 7 - 196 1.1e-29 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 8 - 109 1.4e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 8 - 110 4.5e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 8 - 111 2.7e-14 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 9 - 114 8.7e-13 WDHD1 first WD40 domain
Beta-prop_EML PF23409 10 - 109 6.9e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 13 - 117 1.2e-07 WDR36/Utp21 first beta-propeller
WDR55 PF24796 36 - 195 1.8e-16 WDR55
Beta-prop_THOC3 PF25174 37 - 268 1.8e-30 THOC3 beta-propeller domain
WD40_Gbeta PF25391 48 - 198 2.1e-13 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 49 - 251 3.2e-20 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 57 - 192 2.3e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 61 - 245 2.2e-20 Prp19 WD40 domain
WD40 PF00400 70 - 107 1.4e-10 WD domain, G-beta repeat
EIF3I PF24805 74 - 253 5.8e-08 EIF3I
Beta-prop_WDR90_POC16_2nd PF23393 97 - 191 6e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_1st PF25173 120 - 253 5.7e-15 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 124 - 268 3.6e-07 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 124 - 191 4e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 127 - 273 2.9e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 132 - 246 1e-08 TEP-1 second beta-propeller
WD40 PF00400 203 - 244 1e-05 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 222 - 269 1e-05 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 655
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 2 cut(s) 79, 161
AcoI YGGCCR 2 cut(s) 76, 693
AcsI RAATTY 3 cut(s) 312, 454, 601
AfaI GTAC 3 cut(s) 91, 304, 806
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 2 cut(s) 198, 263
AjnI CCWGG 2 cut(s) 150, 331
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 6 cut(s) 39, 222, 329, 365, 528, 622
AluI AGCT 6 cut(s) 39, 222, 329, 365, 528, 622
Alw21I GWGCWC 1 cut(s) 331
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 3 cut(s) 66, 76, 693
ApeKI GCWGC 6 cut(s) 81, 155, 158, 362, 382, 539
ApoI RAATTY 3 cut(s) 312, 454, 601
AsiGI ACCGGT 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 474
AspLEI GCGC 1 cut(s) 573
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 3 cut(s) 31, 226, 654
AvaII GGWCC 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BalI TGGCCA 1 cut(s) 695
BanII GRGCYC 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BccI CCATC 5 cut(s) 101, 263, 515, 731, 826
BceAI ACGGC 2 cut(s) 48, 342
BciT130I CCWGG 2 cut(s) 152, 333
BciVI GTATCC 2 cut(s) 481, 632
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 574
BfuI GTATCC 2 cut(s) 481, 632
BisI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
BlsI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
Bme1390I CCNGG 2 cut(s) 152, 333
Bme18I GGWCC 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 3 cut(s) 292, 416, 477
BmrFI CCNGG 2 cut(s) 152, 333
BpmI CTGGAG 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 2 cut(s) 136, 211
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 281
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 2 cut(s) 152, 333
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 4 cut(s) 93, 285, 526, 642
BseMII CTCAG 2 cut(s) 657, 834
BseNI ACTGG 1 cut(s) 281
BseXI GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BsgI GTGCAG 2 cut(s) 368, 381
BshFI GGCC 3 cut(s) 68, 78, 695
BshTI ACCGGT 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 2 cut(s) 137, 212
BslFI GGGAC 3 cut(s) 428, 568, 598
BsmFI GGGAC 3 cut(s) 428, 568, 598
BsnI GGCC 3 cut(s) 68, 78, 695
Bsp1286I GDGCHC 1 cut(s) 331
Bsp13I TCCGGA 1 cut(s) 136
BspACI CCGC 2 cut(s) 79, 161
BspANI GGCC 3 cut(s) 68, 78, 695
BspCNI CTCAG 2 cut(s) 658, 833
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 3 cut(s) 292, 416, 477
BspMAI CTGCAG 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 152, 333
Bst4CI ACNGT 8 cut(s) 15, 89, 203, 302, 307, 376, 511, 718
Bst6I CTCTTC 1 cut(s) 709
BstC8I GCNNGC 2 cut(s) 37, 163
BstDEI CTNAG 2 cut(s) 666, 820
BstF5I GGATG 4 cut(s) 93, 285, 526, 642
BstH2I RGCGCY 1 cut(s) 574
BstHHI GCGC 1 cut(s) 573
BstMWI GCNNNNNNNGC 1 cut(s) 577
BstNI CCWGG 2 cut(s) 152, 333
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 2 cut(s) 150, 331
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
BsuI GTATCC 2 cut(s) 481, 632
BsuRI GGCC 3 cut(s) 68, 78, 695
BtsCI GGATG 4 cut(s) 93, 285, 526, 642
BtsI GCAGTG 2 cut(s) 258, 696
BtsIMutI CAGTG 4 cut(s) 258, 372, 696, 714
Cac8I GCNNGC 2 cut(s) 37, 163
CfoI GCGC 1 cut(s) 573
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 415
Csp6I GTAC 3 cut(s) 90, 303, 805
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 2 cut(s) 551, 558
CviQI GTAC 3 cut(s) 90, 303, 805
DdeI CTNAG 2 cut(s) 666, 820
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 2 cut(s) 76, 693
Eam1104I CTCTTC 1 cut(s) 709
EarI CTCTTC 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 415
Eco53kI GAGCTC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 150, 331
EcoT38I GRGCYC 1 cut(s) 331
FaeI CATG 2 cut(s) 554, 561
FaqI GGGAC 3 cut(s) 428, 568, 598
FatI CATG 2 cut(s) 550, 557
FauI CCCGC 1 cut(s) 154
FblI GTMKAC 1 cut(s) 655
Fnu4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FokI GGATG 4 cut(s) 80, 292, 533, 629
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
FspBI CTAG 1 cut(s) 761
GlaI GCGC 1 cut(s) 572
GluI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
GsuI CTGGAG 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 574
HaeIII GGCC 3 cut(s) 68, 78, 695
HapII CCGG 2 cut(s) 137, 212
HhaI GCGC 1 cut(s) 573
Hin1II CATG 2 cut(s) 554, 561
Hin6I GCGC 1 cut(s) 571
HinP1I GCGC 1 cut(s) 571
HincII GTYRAC 2 cut(s) 370, 507
HindII GTYRAC 2 cut(s) 370, 507
HinfI GANTC 5 cut(s) 107, 147, 227, 391, 794
HpaI GTTAAC 2 cut(s) 370, 507
HpaII CCGG 2 cut(s) 137, 212
HphI GGTGA 3 cut(s) 31, 226, 654
Hpy166II GTNNAC 5 cut(s) 370, 507, 656, 677, 826
Hpy188I TCNGA 3 cut(s) 295, 396, 709
Hpy188III TCNNGA 4 cut(s) 137, 479, 721, 761
Hpy8I GTNNAC 5 cut(s) 370, 507, 656, 677, 826
Hpy99I CGWCG 2 cut(s) 19, 34
HpyAV CCTTC 1 cut(s) 758
HpyCH4III ACNGT 8 cut(s) 15, 89, 203, 302, 307, 376, 511, 718
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 5 cut(s) 158, 362, 385, 462, 542
HpyF10VI GCNNNNNNNGC 1 cut(s) 577
HpyF3I CTNAG 2 cut(s) 666, 820
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 2 cut(s) 554, 561
HspAI GCGC 1 cut(s) 571
Kpn2I TCCGGA 1 cut(s) 136
KspAI GTTAAC 2 cut(s) 370, 507
LmnI GCTCC 3 cut(s) 334, 525, 627
Lsp1109I GCAGC 6 cut(s) 68, 142, 170, 369, 374, 526
MaeI CTAG 1 cut(s) 761
MaeII ACGT 1 cut(s) 243
MboII GAAGA 1 cut(s) 696
MhlI GDGCHC 1 cut(s) 331
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 6 cut(s) 193, 312, 454, 470, 601, 782
MluNI TGGCCA 1 cut(s) 695
MnlI CCTC 8 cut(s) 58, 180, 216, 289, 475, 575, 656, 712
Mox20I TGGCCA 1 cut(s) 695
MroI TCCGGA 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 695
MseI TTAA 3 cut(s) 192, 369, 506
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 695
MspA1I CMGCKG 3 cut(s) 81, 161, 365
MspI CCGG 2 cut(s) 137, 212
MspR9I CCNGG 2 cut(s) 152, 333
MvaI CCWGG 2 cut(s) 152, 333
MwoI GCNNNNNNNGC 1 cut(s) 577
NlaIII CATG 2 cut(s) 554, 561
NlaIV GGNNCC 3 cut(s) 292, 416, 477
NspI RCATGY 1 cut(s) 561
OliI CACNNNNGTG 1 cut(s) 377
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 5 cut(s) 107, 147, 227, 391, 794
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 7 cut(s) 80, 83, 157, 160, 364, 384, 541
Psp124BI GAGCTC 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 150, 331
PspGI CCWGG 2 cut(s) 150, 331
PspN4I GGNNCC 3 cut(s) 292, 416, 477
PspPI GGNCC 1 cut(s) 415
PstI CTGCAG 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 3 cut(s) 91, 304, 806
RsaNI GTAC 3 cut(s) 90, 303, 805
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 192, 369, 506
SatI GCNGC 7 cut(s) 79, 82, 156, 159, 363, 383, 540
Sau96I GGNCC 1 cut(s) 415
ScrFI CCNGG 2 cut(s) 152, 333
SduI GDGCHC 1 cut(s) 331
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 6 cut(s) 193, 312, 454, 470, 601, 782
SsiI CCGC 2 cut(s) 79, 161
SspMI CTAG 1 cut(s) 761
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 2 cut(s) 150, 331
TaaI ACNGT 8 cut(s) 15, 89, 203, 302, 307, 376, 511, 718
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 178
TasI AATT 6 cut(s) 193, 312, 454, 470, 601, 782
TatI WGTACW 1 cut(s) 804
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 5 cut(s) 107, 147, 227, 391, 794
Tru1I TTAA 3 cut(s) 192, 369, 506
Tru9I TTAA 3 cut(s) 192, 369, 506
TscAI CASTG 4 cut(s) 258, 379, 703, 721
TseI GCWGC 6 cut(s) 81, 155, 158, 362, 382, 539
TspDTI ATGAA 2 cut(s) 366, 605
TspRI CASTG 4 cut(s) 258, 379, 703, 721
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 3 cut(s) 312, 454, 601
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 655
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.