Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002600

Physical Location & Seq

Genomic Coordinates Forward (+)

8813940 .. 8818344

Gene Structure 4405 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1073600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGTTTGTCCAAGAACGCAACGGACCCAAATCCCCAAAATACTCTCACTCCAACTCCAGAGCGTCGCTTTCCTTCGCTCCGAATCTCGATTTCTCCTCATGGGTTTCCGAGAATCTGTACAAAATCGTCACCGTCGTGCTTCTGATCGCCACCGTCGCCGCCCTCTTCGTCCTCCGTAACATCGGTGACACCGCCGCCTTGCTCTGCTTCGAGACCCAGGCTCAGAACCTCGAGAAGATTCGGATGCCCCAGCTCGAATCCACCGTCAAGACCATCTCCGACACGTCGTCTCCCTACGCCAGCTTCCGATCTGAGAAGTGGGTTGTCGTTTCCGTCTCCGATTACCCGTCTGACTCGCTCAAGAAGCTCGTGAAGCTCAAGGGGTGGCAGGTCCTGGCGATTGGGAACTCCAAAACGCCGTCGGATTGGAGCCTCAAAGGTGCTATCTTTCTGTCCCTTGAACAACAAGCTCAATTGGGTTTTAGGGTTTTGGAGTACTTGCCTTATGATTCTTATGTTAGGAAGAGTGTGGGATACTTGTTTGCGATCCAGCACGGGGCGAAGAAGATCTTCGACGCTGATGATCGCGGAGAGGTGATTGGTGATGATTTGGGTAAACATTTTGATGTGGAATTGATTGGTGAGGGTGCTAGGCAAGAGACTATATTGCAGTATAGCCATGAGAATCCCAACAGGACCATTGTGAACCCGTATATTCATTTCGGGCAACGCTCTGTGTGGCCGAGAGGTTTGCCATTGGAGAATGTTGGTGAGCTTGGCCATGAAGAGTTCTACACTGAGGTTTTTGGTGGAAAGCAGTTTATACAACAGGGTATATCCAATGGACTTCCTGATGTCGATTCTGTGTTTTATTTTACACGAAAATCAGGTTTGGAAGCGTTTGATATCAGGTTTGATGACCATGCCCCAAAAGTGGCATTGCCGCAGGGGACGATGGTGCCTGTTAACTCTTTCAATACAATTTACCATTCGTCGGCATTTTGGGGTTTGATGCTTCCTGTTTCTGTTAGCACAATGGCTTCTGATATATTAAGGGGTTACTGGGGGCAAAGGCTTTTGTGGGAAATAGGTGGATATGTTGTAGTTTATCCCCCAACTGTTCATCGGTATGATAGAATTCAGGCATACCCCTTTTCTGAAGAGAAAGATCTTCATGTTAATGTAGGACGTTTGATCAAATTTTTGGTTTCATGGAGATCGGGTAAGCATAGGTTGTTTGAAAAGATTTTGGAGTTAAGTTTTGTCATGGCAGAAGAGGGGTTTTGGACTGAGAAGGATCTGAAGTTTACCGCTGCTTGGCTTCATGACTTAATTGCTGTAGGGTATCAGCAGCCTAGATTAATGTCATTGGAATTGGATCGGCCACGGGCAAATATTGGTCATGGGGATACAAAAGAGTTTGTCCCGCAAAAATTTCCATCAGTTCATCTTGGAGTTGAGGAAACAGGAACAGTGAACTATGAAATTGGAAATTTGATTAGATGGAGGAAGAATTTTGGGAATGTTGTGCTTATCATGTTTTGCAGTGGACCTGTGGAACGTACTGCTTTGGAATGGAGATTGCTTTATGGGCGGATATTTAAAACTGTGATCATTTTGTCTGATCTAAAGAACACCGATCTTGCTGTTGAAGAAGGAAAATTGGAGAATGTATATAAGTACATGCCCAAAATTTTTGATCGATATTCCGGGGCAGATGGATTTTTATTTCTTCAGGACAATACTATTCTTAATTACTGGAATCTTCTACAGGCAGACAAAACTAAATTGTGGATCACAAATGAGGTACCCAAGTCTTGGAGTACTGTGTCAACAAAAGACAACTCAGAAGAATGGTTTTCAAAGCAAGCAGGCATGGTAAAGAAGGTTGTTAGCATGATGCCAGTTCACTTCCAAGTCAGTTATAAGAACAGTGTAACTAGTCGCAAGAGCGTCACATTATGCAGTTCTGAGGTGTTCTACATCCCTCGTCGGTTTGTAGCTGACTTCGTCGATCTTGTTAATCTAGTTGGGAATCTAGAAATTCATTACAAGGTTGCCATTCCAATGTTCTTTCAAGCATTAGATTCACCTCAGAACTTTGACTCGGTGCTCAGTACAATGATATATGAGGAACAACTACCTTCTACGAATTCTTCATCCTTGTATTCGGCTAAAGTACCTGCGGTTCACCCATGGAGTGTGACAAGCGAACAAGAGTTCATTAAACTAATAAGAGTAATGGCAGAAGGTGACCCTCTCCTAATGGAGTTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0006073 GO:0006109 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009664 GO:0009832 GO:0009889 GO:0009987 GO:0010383 GO:0010556 GO:0010675 GO:0010962 GO:0010981 GO:0012505 GO:0016043 GO:0016051 GO:0019222 GO:0030243 GO:0030244 GO:0030312 GO:0031323 GO:0031326 GO:0031410 GO:0031982 GO:0031984 GO:0032881 GO:0032885 GO:0032950 GO:0032951 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0050789 GO:0050794 GO:0051128 GO:0051273 GO:0051274 GO:0052324 GO:0052541 GO:0060255 GO:0065007 GO:0070589 GO:0070592 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0080090 GO:0097708 GO:0098791 GO:1901576 GO:1903338 GO:2000112 GO:2001006 GO:2001009

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

STELLO

Protein Analysis

760

Amino Acids

86.44

Weight (kDa)

6.32

Isoelectric Point (pI)

34.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
STELLO	PF03385	348 - 460	1.8e-08	STELLO glycosyltransferases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002600)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41770 AT3G57420
fragaria_vesca	FvH4_6g23790 FvH4_6g23790 FvH4_7g01460
malus_domestica	MD07G1018300.v1.1 MD12G1073600.v1.1 MD14G1071900.v1.1
prunus_persica	Prupe.2G020200_v2.0.a1 Prupe.7G061300_v2.0.a1
pyrus_communis	pycom07g01440 pycom12g06360 pycom14g06170
rosa_chinensis	RchiOBHm_Chr1g0317301 RchiOBHm_Chr3g0482321
rosa_laevigata	RLG00000023359 RLG00000029570 RLG00000030608
rosa_multiflora	Rmu_co8232473.1_g000001 Rmu_co8457415.1_g000001 Rmu_sc0000135.1_g000005 Rmu_sc0013869.1_g000004 Rmu_sc0015957.1_g000002
rosa_roxburghii	Rroxscaffold_4G00330510 Rroxscaffold_6G00399430 Rroxscaffold_6G00399450 Rroxscaffold_6G00399930
rosa_rugosa	Rorug01G0015700 Rorug03G0195800 Rorug03G0195800
rosa_samantha	Rh1AG027100 Rh1BG023000 Rh1CG025100 Rh1DG046100 Rh3AG247400 Rh3BG281900 Rh3CG278900 Rh3DG274700
rosa_wichuraiana	Rw1G001870 Rw3G022180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1926
Acc36I	ACCTGC	2 cut(s)	379, 2191
Acc65I	GGTACC	1 cut(s)	1807
AccB1I	GGYRCC	2 cut(s)	958, 1807
AccB7I	CCANNNNNTGG	1 cut(s)	1818
AccII	CGCG	1 cut(s)	588
AciI	CCGC	9 cut(s)	159, 192, 195, 588, 944, 1311, 1427, 1594, 2186
AclWI	GGATC	4 cut(s)	541, 1305, 1386, 1802
AcoI	YGGCCR	3 cut(s)	740, 778, 1382
AcsI	RAATTY	8 cut(s)	1137, 1199, 1433, 1492, 1513, 1692, 2043, 2152
AcuI	CTGAAG	3 cut(s)	1179, 1322, 1718
AfaI	GTAC	8 cut(s)	119, 497, 1564, 1682, 1809, 1825, 2119, 2181
AfiI	CCNNNNNNNGG	5 cut(s)	556, 934, 994, 1317, 1818
AflIII	ACRYGT	1 cut(s)	282
AgsI	TTSAA	6 cut(s)	461, 976, 1241, 1652, 1863, 2078
AhdI	GACNNNNNGTC	1 cut(s)	286
AhlI	ACTAGT	1 cut(s)	1940
AjiI	CACGTC	1 cut(s)	285
AjnI	CCWGG	2 cut(s)	216, 393
AleI	CACNNNNGTG	1 cut(s)	134
AluBI	AGCT	7 cut(s)	253, 303, 367, 376, 470, 775, 2003
AluI	AGCT	7 cut(s)	253, 303, 367, 376, 470, 775, 2003
Alw21I	GWGCWC	1 cut(s)	2115
Alw26I	GTCTC	4 cut(s)	206, 294, 340, 653
AlwI	GGATC	4 cut(s)	541, 1305, 1386, 1802
AlwNI	CAGNNNCTG	1 cut(s)	394
Ama87I	CYCGRG	1 cut(s)	230
AoxI	GGCC	3 cut(s)	740, 778, 1382
ApeKI	GCWGC	2 cut(s)	1313, 1351
ApoI	RAATTY	8 cut(s)	1137, 1199, 1433, 1492, 1513, 1692, 2043, 2152
ArsI	GACNNNNNNTTYG	2 cut(s)	249, 281
AseI	ATTAAT	1 cut(s)	1361
Asp700I	GAANNNNTTC	1 cut(s)	569
Asp718I	GGTACC	1 cut(s)	1807
AspS9I	GGNCC	4 cut(s)	23, 391, 696, 1550
AsuC2I	CCSGG	1 cut(s)	1711
AsuHPI	GGTGA	9 cut(s)	121, 197, 607, 614, 653, 782, 2082, 2183, 2264
AvaI	CYCGRG	1 cut(s)	230
AvaII	GGWCC	4 cut(s)	23, 391, 696, 1550
BaeI	ACNNNNGTAYC	2 cut(s)	1799, 1832
BalI	TGGCCA	1 cut(s)	780
BanI	GGYRCC	2 cut(s)	958, 1807
BauI	CACGAG	1 cut(s)	368
Bbv12I	GWGCWC	1 cut(s)	2115
BbvI	GCAGC	2 cut(s)	1300, 1363
BccI	CCATC	5 cut(s)	281, 949, 1447, 1497, 1712
BceAI	ACGGC	1 cut(s)	403
BcgI	CGANNNNNNTGC	2 cut(s)	733, 767
BciT130I	CCWGG	2 cut(s)	218, 395
BciVI	GTATCC	2 cut(s)	527, 1402
BclI	TGATCA	2 cut(s)	1194, 1611
BcnI	CCSGG	1 cut(s)	1711
BcoDI	GTCTC	4 cut(s)	206, 294, 340, 653
BcuI	ACTAGT	1 cut(s)	1940
BfaI	CTAG	5 cut(s)	651, 1356, 1941, 2027, 2039
BfmI	CTRYAG	2 cut(s)	1338, 1769
BfuAI	ACCTGC	2 cut(s)	379, 2191
BfuI	GTATCC	2 cut(s)	527, 1402
BglII	AGATCT	2 cut(s)	567, 1168
BisI	GCNGC	5 cut(s)	159, 195, 944, 1314, 1352
BlsI	GCNGC	5 cut(s)	160, 196, 945, 1315, 1353
BmcAI	AGTACT	2 cut(s)	497, 1825
Bme1390I	CCNGG	3 cut(s)	218, 395, 1711
Bme18I	GGWCC	4 cut(s)	23, 391, 696, 1550
BmeRI	GACNNNNNGTC	1 cut(s)	286
BmeT110I	CYCGRG	1 cut(s)	230
BmgBI	CACGTC	1 cut(s)	285
BmgT120I	GGNCC	4 cut(s)	23, 391, 696, 1550
BmiI	GGNNCC	4 cut(s)	25, 431, 960, 1809
BmrFI	CCNGG	3 cut(s)	218, 395, 1711
BmrI	ACTGGG	1 cut(s)	1072
BmsI	GCATC	3 cut(s)	234, 1002, 1890
BmuI	ACTGGG	1 cut(s)	1072
BpmI	CTGGAG	1 cut(s)	40
BpuEI	CTTGAG	2 cut(s)	344, 362
BpuMI	CCSGG	1 cut(s)	1711
Bsa29I	ATCGAT	1 cut(s)	1702
BsaI	GGTCTC	1 cut(s)	206
BsaJI	CCNNGG	4 cut(s)	216, 1385, 1710, 2195
BsaXI	ACNNNNNCTCC	8 cut(s)	32, 38, 62, 68, 274, 304, 1207, 1237
Bsc4I	CCNNNNNNNGG	5 cut(s)	556, 934, 994, 1317, 1818
Bse1I	ACTGG	3 cut(s)	1067, 1763, 1904
Bse3DI	GCAATG	1 cut(s)	938
BseBI	CCWGG	2 cut(s)	218, 395
BseCI	ATCGAT	1 cut(s)	1702
BseDI	CCNNGG	4 cut(s)	216, 1385, 1710, 2195
BseGI	GGATG	3 cut(s)	249, 1983, 2159
BseLI	CCNNNNNNNGG	5 cut(s)	556, 934, 994, 1317, 1818
BseMI	GCAATG	1 cut(s)	938
BseMII	CTCAG	8 cut(s)	236, 303, 789, 1281, 1860, 1962, 2108, 2128
BseNI	ACTGG	3 cut(s)	1067, 1763, 1904
BseRI	GAGGAG	1 cut(s)	85
BseXI	GCAGC	2 cut(s)	1300, 1363
BseYI	CCCAGC	1 cut(s)	249
Bsh1236I	CGCG	1 cut(s)	588
BshFI	GGCC	3 cut(s)	742, 780, 1384
BshNI	GGYRCC	2 cut(s)	958, 1807
BshVI	ATCGAT	1 cut(s)	1702
BsiHKAI	GWGCWC	1 cut(s)	2115
BsiHKCI	CYCGRG	1 cut(s)	230
BsiSI	CCGG	1 cut(s)	1710
BslFI	GGGAC	3 cut(s)	439, 964, 1409
BslI	CCNNNNNNNGG	5 cut(s)	556, 934, 994, 1317, 1818
BsmAI	GTCTC	4 cut(s)	206, 294, 340, 653
BsmBI	CGTCTC	2 cut(s)	294, 340
BsmFI	GGGAC	3 cut(s)	439, 964, 1409
BsnI	GGCC	3 cut(s)	742, 780, 1384
Bso31I	GGTCTC	1 cut(s)	206
BsoBI	CYCGRG	1 cut(s)	230
Bsp1286I	GDGCHC	1 cut(s)	2115
Bsp1407I	TGTACA	1 cut(s)	117
Bsp19I	CCATGG	1 cut(s)	2195
BspACI	CCGC	9 cut(s)	159, 192, 195, 588, 944, 1311, 1427, 1594, 2186
BspANI	GGCC	3 cut(s)	742, 780, 1384
BspCNI	CTCAG	8 cut(s)	235, 304, 790, 1282, 1859, 1963, 2107, 2127
BspDI	ATCGAT	1 cut(s)	1702
BspFNI	CGCG	1 cut(s)	588
BspHI	TCATGA	1 cut(s)	1324
BspLI	GGNNCC	4 cut(s)	25, 431, 960, 1809
BspMI	ACCTGC	2 cut(s)	379, 2191
BspPI	GGATC	4 cut(s)	541, 1305, 1386, 1802
BspT107I	GGYRCC	2 cut(s)	958, 1807
BspTNI	GGTCTC	1 cut(s)	206
BsrDI	GCAATG	1 cut(s)	938
BsrGI	TGTACA	1 cut(s)	117
BsrI	ACTGG	3 cut(s)	1067, 1763, 1904
BssECI	CCNNGG	4 cut(s)	216, 1385, 1710, 2195
BssSI	CACGAG	1 cut(s)	368
BssT1I	CCWWGG	1 cut(s)	2195
Bst2BI	CACGAG	1 cut(s)	368
Bst2UI	CCWGG	2 cut(s)	218, 395
Bst4CI	ACNGT	8 cut(s)	133, 154, 265, 1120, 1474, 1609, 1828, 1934
Bst6I	CTCTTC	5 cut(s)	170, 518, 780, 1155, 1269
BstAUI	TGTACA	1 cut(s)	117
BstC8I	GCNNGC	3 cut(s)	301, 1869, 1873
BstDEI	CTNAG	8 cut(s)	222, 312, 798, 1290, 1846, 1971, 2094, 2114
BstDSI	CCRYGG	2 cut(s)	1385, 2195
BstEII	GGTNACC	1 cut(s)	2252
BstF5I	GGATG	3 cut(s)	249, 1983, 2159
BstFNI	CGCG	1 cut(s)	588
BstMAI	GTCTC	4 cut(s)	206, 294, 340, 653
BstMWI	GCNNNNNNNGC	4 cut(s)	155, 364, 373, 1591
BstNI	CCWGG	2 cut(s)	218, 395
BstNSI	RCATGY	1 cut(s)	1687
BstPI	GGTNACC	1 cut(s)	2252
BstSCI	CCNGG	3 cut(s)	216, 393, 1709
BstSFI	CTRYAG	2 cut(s)	1338, 1769
BstUI	CGCG	1 cut(s)	588
BstV1I	GCAGC	2 cut(s)	1300, 1363
BstX2I	RGATCY	3 cut(s)	567, 1168, 1297
BstYI	RGATCY	3 cut(s)	567, 1168, 1297
Bsu15I	ATCGAT	1 cut(s)	1702
BsuI	GTATCC	2 cut(s)	527, 1402
BsuRI	GGCC	3 cut(s)	742, 780, 1384
BsuTUI	ATCGAT	1 cut(s)	1702
BtgI	CCRYGG	2 cut(s)	1385, 2195
BtrI	CACGTC	1 cut(s)	285
BtsCI	GGATG	3 cut(s)	249, 1983, 2159
BtsI	GCAGTG	1 cut(s)	1552
BtsIMutI	CAGTG	4 cut(s)	795, 1479, 1552, 1939
BveI	ACCTGC	2 cut(s)	379, 2191
Cac8I	GCNNGC	3 cut(s)	301, 1869, 1873
CaiI	CAGNNNCTG	1 cut(s)	394
CciI	TCATGA	1 cut(s)	1324
Cfr13I	GGNCC	4 cut(s)	23, 391, 696, 1550
ClaI	ATCGAT	1 cut(s)	1702
CseI	GACGC	3 cut(s)	51, 584, 1942
Csp6I	GTAC	8 cut(s)	118, 496, 1563, 1681, 1808, 1824, 2118, 2180
CviQI	GTAC	8 cut(s)	118, 496, 1563, 1681, 1808, 1824, 2118, 2180
DdeI	CTNAG	8 cut(s)	222, 312, 798, 1290, 1846, 1971, 2094, 2114
DraI	TTTAAA	1 cut(s)	1603
DriI	GACNNNNNGTC	1 cut(s)	286
EaeI	YGGCCR	3 cut(s)	740, 778, 1382
Eam1104I	CTCTTC	5 cut(s)	170, 518, 780, 1155, 1269
Eam1105I	GACNNNNNGTC	1 cut(s)	286
EarI	CTCTTC	5 cut(s)	170, 518, 780, 1155, 1269
EciI	GGCGGA	1 cut(s)	1609
Eco130I	CCWWGG	1 cut(s)	2195
Eco31I	GGTCTC	1 cut(s)	206
Eco32I	GATATC	1 cut(s)	907
Eco47I	GGWCC	4 cut(s)	23, 391, 696, 1550
Eco57I	CTGAAG	3 cut(s)	1179, 1322, 1718
Eco88I	CYCGRG	1 cut(s)	230
Eco91I	GGTNACC	1 cut(s)	2252
EcoO109I	RGGNCCY	1 cut(s)	391
EcoO65I	GGTNACC	1 cut(s)	2252
EcoRI	GAATTC	2 cut(s)	1137, 2152
EcoRII	CCWGG	2 cut(s)	216, 393
EcoRV	GATATC	1 cut(s)	907
EcoT14I	CCWWGG	1 cut(s)	2195
ErhI	CCWWGG	1 cut(s)	2195
Esp3I	CGTCTC	2 cut(s)	294, 340
FalI	AAGNNNNNCTT	2 cut(s)	554, 586
FaqI	GGGAC	3 cut(s)	439, 964, 1409
FauI	CCCGC	1 cut(s)	1434
FbaI	TGATCA	2 cut(s)	1194, 1611
Fnu4HI	GCNGC	5 cut(s)	159, 195, 944, 1314, 1352
FokI	GGATG	3 cut(s)	256, 1970, 2146
Fsp4HI	GCNGC	5 cut(s)	159, 195, 944, 1314, 1352
FspBI	CTAG	5 cut(s)	651, 1356, 1941, 2027, 2039
GluI	GCNGC	5 cut(s)	159, 195, 944, 1314, 1352
GsaI	CCCAGC	1 cut(s)	253
GsuI	CTGGAG	1 cut(s)	40
HaeIII	GGCC	3 cut(s)	742, 780, 1384
HapII	CCGG	1 cut(s)	1710
HgaI	GACGC	3 cut(s)	51, 584, 1942
HincII	GTYRAC	2 cut(s)	967, 1833
HindII	GTYRAC	2 cut(s)	967, 1833
HpaI	GTTAAC	1 cut(s)	967
HpaII	CCGG	1 cut(s)	1710
HphI	GGTGA	9 cut(s)	121, 197, 607, 614, 653, 782, 2082, 2183, 2264
Hpy166II	GTNNAC	9 cut(s)	617, 706, 967, 1308, 1477, 1550, 1833, 1909, 2191
Hpy8I	GTNNAC	9 cut(s)	617, 706, 967, 1308, 1477, 1550, 1833, 1909, 2191
Hpy99I	CGWCG	9 cut(s)	67, 137, 158, 289, 424, 578, 997, 1995, 2015
HpyAV	CCTTC	6 cut(s)	82, 1288, 1649, 1879, 2154, 2243
HpyCH4III	ACNGT	8 cut(s)	133, 154, 265, 1120, 1474, 1609, 1828, 1934
HpyCH4IV	ACGT	3 cut(s)	284, 1189, 1561
HpyCH4V	TGCA	3 cut(s)	670, 1545, 1965
HpyF10VI	GCNNNNNNNGC	4 cut(s)	155, 364, 373, 1591
HpyF3I	CTNAG	8 cut(s)	222, 312, 798, 1290, 1846, 1971, 2094, 2114
HpySE526I	ACGT	3 cut(s)	284, 1189, 1561
KpnI	GGTACC	1 cut(s)	1811
Ksp22I	TGATCA	2 cut(s)	1194, 1611
KspAI	GTTAAC	1 cut(s)	967
LmnI	GCTCC	2 cut(s)	82, 429
Lsp1109I	GCAGC	2 cut(s)	1300, 1363
LweI	GCATC	3 cut(s)	234, 1002, 1890
MaeI	CTAG	5 cut(s)	651, 1356, 1941, 2027, 2039
MaeII	ACGT	3 cut(s)	284, 1189, 1561
MaeIII	GTNAC	8 cut(s)	127, 176, 185, 1058, 1936, 1954, 2203, 2252
MfeI	CAATTG	1 cut(s)	473
MflI	RGATCY	3 cut(s)	567, 1168, 1297
MhlI	GDGCHC	1 cut(s)	2115
MlsI	TGGCCA	1 cut(s)	780
MluNI	TGGCCA	1 cut(s)	780
MlyI	GAGTC	2 cut(s)	347, 2099
MmeI	TCCRAC	3 cut(s)	75, 303, 402
Mox20I	TGGCCA	1 cut(s)	780
MroXI	GAANNNNTTC	1 cut(s)	569
MscI	TGGCCA	1 cut(s)	780
MslI	CAYNNNNRTG	5 cut(s)	134, 624, 1128, 1179, 2066
Msp20I	TGGCCA	1 cut(s)	780
MspA1I	CMGCKG	1 cut(s)	1313
MspI	CCGG	1 cut(s)	1710
MspR9I	CCNGG	3 cut(s)	218, 395, 1711
MunI	CAATTG	1 cut(s)	473
MvaI	CCWGG	2 cut(s)	218, 395
MvnI	CGCG	1 cut(s)	588
MwoI	GCNNNNNNNGC	4 cut(s)	155, 364, 373, 1591
NciI	CCSGG	1 cut(s)	1711
NcoI	CCATGG	1 cut(s)	2195
NlaIV	GGNNCC	4 cut(s)	25, 431, 960, 1809
NmeAIII	GCCGAG	1 cut(s)	768
NmuCI	GTSAC	5 cut(s)	127, 185, 1954, 2203, 2252
NspI	RCATGY	1 cut(s)	1687
OliI	CACNNNNGTG	1 cut(s)	134
PaeR7I	CTCGAG	1 cut(s)	230
PagI	TCATGA	1 cut(s)	1324
PcsI	WCGNNNNNNNCGW	2 cut(s)	132, 261
PdmI	GAANNNNTTC	1 cut(s)	569
PflFI	GACNNNGTC	1 cut(s)	2009
PflMI	CCANNNNNTGG	1 cut(s)	1818
PkrI	GCNGC	5 cut(s)	160, 196, 945, 1315, 1353
PleI	GAGTC	2 cut(s)	347, 2099
PpsI	GAGTC	2 cut(s)	347, 2099
PpuMI	RGGWCCY	1 cut(s)	391
PshBI	ATTAAT	1 cut(s)	1361
PsiI	TTATAA	1 cut(s)	1926
Psp5II	RGGWCCY	1 cut(s)	391
Psp6I	CCWGG	2 cut(s)	216, 393
PspEI	GGTNACC	1 cut(s)	2252
PspFI	CCCAGC	1 cut(s)	249
PspGI	CCWGG	2 cut(s)	216, 393
PspN4I	GGNNCC	4 cut(s)	25, 431, 960, 1809
PspPI	GGNCC	4 cut(s)	23, 391, 696, 1550
PspPPI	RGGWCCY	1 cut(s)	391
PsrI	GAACNNNNNNTAC	2 cut(s)	2172, 2204
PstNI	CAGNNNCTG	1 cut(s)	394
PsuI	RGATCY	3 cut(s)	567, 1168, 1297
PsyI	GACNNNGTC	1 cut(s)	2009
RsaI	GTAC	8 cut(s)	119, 497, 1564, 1682, 1809, 1825, 2119, 2181
RsaNI	GTAC	8 cut(s)	118, 496, 1563, 1681, 1808, 1824, 2118, 2180
RseI	CAYNNNNRTG	5 cut(s)	134, 624, 1128, 1179, 2066
SatI	GCNGC	5 cut(s)	159, 195, 944, 1314, 1352
Sau96I	GGNCC	4 cut(s)	23, 391, 696, 1550
ScaI	AGTACT	2 cut(s)	497, 1825
SchI	GAGTC	2 cut(s)	347, 2099
ScrFI	CCNGG	3 cut(s)	218, 395, 1711
SduI	GDGCHC	1 cut(s)	2115
SfaNI	GCATC	3 cut(s)	234, 1002, 1890
SfcI	CTRYAG	2 cut(s)	1338, 1769
Sfr274I	CTCGAG	1 cut(s)	230
SinI	GGWCC	4 cut(s)	23, 391, 696, 1550
SlaI	CTCGAG	1 cut(s)	230
SmiMI	CAYNNNNRTG	5 cut(s)	134, 624, 1128, 1179, 2066
SmlI	CTYRAG	3 cut(s)	230, 359, 377
SmoI	CTYRAG	3 cut(s)	230, 359, 377
SpeI	ACTAGT	1 cut(s)	1940
SsiI	CCGC	9 cut(s)	159, 192, 195, 588, 944, 1311, 1427, 1594, 2186
SspI	AATATT	1 cut(s)	1396
SspMI	CTAG	5 cut(s)	651, 1356, 1941, 2027, 2039
StyD4I	CCNGG	3 cut(s)	216, 393, 1709
StyI	CCWWGG	1 cut(s)	2195
TaaI	ACNGT	8 cut(s)	133, 154, 265, 1120, 1474, 1609, 1828, 1934
TaiI	ACGT	3 cut(s)	287, 1192, 1564
TaqI	TCGA	8 cut(s)	87, 210, 231, 255, 573, 858, 1702, 2013
TatI	WGTACW	5 cut(s)	117, 495, 1680, 1823, 2117
TauI	GCSGC	3 cut(s)	161, 197, 946
TscAI	CASTG	4 cut(s)	802, 1479, 1552, 1939
TseFI	GTSAC	5 cut(s)	127, 185, 1954, 2203, 2252
TseI	GCWGC	2 cut(s)	1313, 1351
Tsp45I	GTSAC	5 cut(s)	127, 185, 1954, 2203, 2252
TspGWI	ACGGA	3 cut(s)	36, 164, 322
TspRI	CASTG	4 cut(s)	802, 1479, 1552, 1939
Tth111I	GACNNNGTC	1 cut(s)	2009
Van91I	CCANNNNNTGG	1 cut(s)	1818
VpaK11BI	GGWCC	4 cut(s)	23, 391, 696, 1550
VspI	ATTAAT	1 cut(s)	1361
XapI	RAATTY	8 cut(s)	1137, 1199, 1433, 1492, 1513, 1692, 2043, 2152
XbaI	TCTAGA	1 cut(s)	2038
XceI	RCATGY	1 cut(s)	1687
XhoI	CTCGAG	1 cut(s)	230
XmnI	GAANNNNTTC	1 cut(s)	569
XspI	CTAG	5 cut(s)	651, 1356, 1941, 2027, 2039
ZrmI	AGTACT	2 cut(s)	497, 1825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.