Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007794

Physical Location & Seq

Genomic Coordinates Reverse (-)

12154707 .. 12158938

Gene Structure 4232 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1093300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGAAGGATACACGCAGACGCAGTATTCTCGTCAGAGCTGAGTCATACACAAAAGAAAACATAAAAACCAAAGATACAAGAGAAAGACCCTCAAATGAAGCTTCAGCAATGGCGTTAATGGCAACCAGAACAAACCTCTTTAGGCTCCTCACTACAAAAGCACCGATTTTGGGATCTGGGTCTTCCTCCAATGGCCTCAAGGGACCCGTTCGGGCCGACCGGAGCGGTGGAGCCGTGTCAACTTCGTCGTATTCGACCTCTGCGGTGGCGCAGCAGCCTGAGTCCGGGACCATTGGGTCTAGGCCGCCGCACATGGGGCAGGAGATATTGGGTGTGAAAGATTACGAAGATTATAGAAGATCTTTGTATGGTGAAATCACTCACAAGGCTTTGCTAGTTGATGCTGTTGGTACCCTTGTTGTTCCTTCCCAGCCAATGGCTCAGATATATAGGAAGATAGGCGAGAAATACGGAGTGGAGTACTCAGAGACCGAGATTTTGAACAGATACAGAAGAGCTTACGAGCAGCCTTGGGGAAGATCTCGTCTCAGATATGTAAATGATGGGAGACCCTTCTGGCAATATATAGTCAGTTCTTCCACTGGCTGTTCTGATTCTCAGTACTTTGAAGAACTTTATAACTACTATACGACTGACAAGGCTTGGCACCTCTGTGATCCTGATGCTGAGAAGGTGTTTGAAGCTCTGAGGAAAGCAGGTGTTAAAGTAGCTGTTGTATCAAATTTTGATACCCGATTAAGGCCTCTTTTACATGCTCTACATTGTGAACATTGGTTTGATGCAGTAGCAGTGTCAGCCGAAGTTGAAGCAGAGAAGCCAAATCCAACAATATTTCTTAAAGCTTGTGAATTATTGGGAGTAAAACCTGAGGATGCTGTGCATGTAGGTGATGATCGTAGGAATGATATATGGGGTGCTAGAGATGCAGGCTGTGATGCTTGGCTTTGGGGAAGTGATGTTCACTCCTTCAAGGAGGTGGCTCAGAGGGTAGGGGTCCAGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAD_2

Protein Analysis

342

Amino Acids

38.25

Weight (kDa)

8.16

Isoelectric Point (pI)

43.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	129 - 317	1.9e-24	haloacid dehalogenase-like hydrolase
HAD_2	PF13419	144 - 319	4.8e-16	Haloacid dehalogenase-like hydrolase
Hydrolase_like	PF13242	279 - 334	3.1e-09	HAD-hyrolase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41250
fragaria_vesca	FvH4_6g25490 FvH4_6g25490 FvH4_6g25490 FvH4_6g25490 FvH4_6g25490
malus_domestica	MD12G1093300.v1.1 MD14G1085300.v1.1
prunus_persica	Prupe.7G041200_v2.0.a1 Prupe.7G041200_v2.0.a1 Prupe.7G041200_v2.0.a1 Prupe.7G041200_v2.0.a1
pyrus_communis	pycom12g07860 pycom14g07490
rosa_chinensis	RchiOBHm_Chr2g0125431
rosa_laevigata	RLG00000018866
rosa_multiflora	Rmu_sc0005508.1_g000001
rosa_roxburghii	Rroxscaffold_2G00118000
rosa_rugosa	Rorug02G0257400
rosa_samantha	Rh2AG316300 Rh2BG323800 Rh2CG306000 Rh2DG342400
rosa_wichuraiana	Rw2G025500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	639
AarI	CACCTGC	1 cut(s)	707
AasI	GACNNNNNNGTC	1 cut(s)	295
Acc36I	ACCTGC	1 cut(s)	707
Acc65I	GGTACC	1 cut(s)	410
AccB1I	GGYRCC	2 cut(s)	410, 666
AccB7I	CCANNNNNTGG	1 cut(s)	436
AccBSI	CCGCTC	1 cut(s)	225
AciI	CCGC	4 cut(s)	225, 263, 305, 308
AclWI	GGATC	2 cut(s)	181, 671
AcsI	RAATTY	1 cut(s)	742
AcuI	CTGAAG	1 cut(s)	87
AfaI	GTAC	3 cut(s)	412, 482, 623
AfiI	CCNNNNNNNGG	5 cut(s)	170, 284, 314, 436, 759
AgsI	TTSAA	5 cut(s)	502, 629, 701, 827, 991
AjnI	CCWGG	1 cut(s)	1017
AleI	CACNNNNGTG	1 cut(s)	672
AloI	GAACNNNNNNTCC	2 cut(s)	963, 995
AluBI	AGCT	6 cut(s)	38, 101, 518, 704, 731, 863
AluI	AGCT	6 cut(s)	38, 101, 518, 704, 731, 863
Alw26I	GTCTC	3 cut(s)	482, 551, 562
AlwI	GGATC	2 cut(s)	181, 671
AoxI	GGCC	4 cut(s)	193, 213, 302, 761
ApeKI	GCWGC	3 cut(s)	271, 274, 526
ApoI	RAATTY	1 cut(s)	742
ArsI	GACNNNNNNTTYG	2 cut(s)	481, 513
Asp718I	GGTACC	1 cut(s)	410
AspLEI	GCGC	1 cut(s)	271
AspS9I	GGNCC	4 cut(s)	203, 213, 288, 1015
AsuC2I	CCSGG	1 cut(s)	286
AsuHPI	GGTGA	2 cut(s)	383, 920
AvaII	GGWCC	3 cut(s)	203, 288, 1015
AxyI	CCTNAGG	1 cut(s)	888
BaeI	ACNNNNGTAYC	2 cut(s)	402, 435
BanI	GGYRCC	2 cut(s)	410, 666
BbsI	GAAGAC	1 cut(s)	174
BbvI	GCAGC	3 cut(s)	283, 286, 538
BccI	CCATC	1 cut(s)	557
BceAI	ACGGC	1 cut(s)	218
BcgI	CGANNNNNNTGC	2 cut(s)	10, 44
BciT130I	CCWGG	1 cut(s)	1019
BcnI	CCSGG	1 cut(s)	286
BcoDI	GTCTC	3 cut(s)	482, 551, 562
BfaI	CTAG	3 cut(s)	300, 395, 939
BfuAI	ACCTGC	1 cut(s)	707
BglII	AGATCT	2 cut(s)	359, 539
BisI	GCNGC	5 cut(s)	272, 275, 305, 308, 527
BlsI	GCNGC	5 cut(s)	273, 276, 306, 309, 528
BmcAI	AGTACT	2 cut(s)	482, 623
Bme1390I	CCNGG	2 cut(s)	286, 1019
Bme18I	GGWCC	3 cut(s)	203, 288, 1015
BmgT120I	GGNCC	4 cut(s)	203, 213, 288, 1015
BmiI	GGNNCC	8 cut(s)	146, 204, 205, 232, 289, 412, 668, 1016
BmrFI	CCNGG	2 cut(s)	286, 1019
BmsI	GCATC	6 cut(s)	391, 673, 790, 883, 934, 946
BpiI	GAAGAC	1 cut(s)	174
BpuEI	CTTGAG	1 cut(s)	182
BpuMI	CCSGG	1 cut(s)	286
BsaI	GGTCTC	2 cut(s)	482, 562
BsaJI	CCNNGG	1 cut(s)	530
BsaWI	WCCGGW	1 cut(s)	219
Bsc4I	CCNNNNNNNGG	5 cut(s)	170, 284, 314, 436, 759
Bse1I	ACTGG	1 cut(s)	607
Bse21I	CCTNAGG	1 cut(s)	888
Bse3DI	GCAATG	1 cut(s)	114
BseBI	CCWGG	1 cut(s)	1019
BseDI	CCNNGG	1 cut(s)	530
BseGI	GGATG	1 cut(s)	898
BseLI	CCNNNNNNNGG	5 cut(s)	170, 284, 314, 436, 759
BseMI	GCAATG	1 cut(s)	114
BseNI	ACTGG	1 cut(s)	607
BseRI	GAGGAG	1 cut(s)	137
BseXI	GCAGC	3 cut(s)	283, 286, 538
BseYI	CCCAGC	1 cut(s)	429
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	4 cut(s)	195, 215, 304, 763
BshNI	GGYRCC	2 cut(s)	410, 666
BsiEI	CGRYCG	1 cut(s)	220
BsiSI	CCGG	2 cut(s)	220, 285
BslFI	GGGAC	2 cut(s)	216, 301
BslI	CCNNNNNNNGG	5 cut(s)	170, 284, 314, 436, 759
BsmAI	GTCTC	3 cut(s)	482, 551, 562
BsmBI	CGTCTC	1 cut(s)	551
BsmFI	GGGAC	2 cut(s)	216, 301
BsnI	GGCC	4 cut(s)	195, 215, 304, 763
Bso31I	GGTCTC	2 cut(s)	482, 562
Bsp143I	GATC	5 cut(s)	173, 359, 539, 676, 913
BspACI	CCGC	4 cut(s)	225, 263, 305, 308
BspANI	GGCC	4 cut(s)	195, 215, 304, 763
BspLI	GGNNCC	8 cut(s)	146, 204, 205, 232, 289, 412, 668, 1016
BspMI	ACCTGC	1 cut(s)	707
BspPI	GGATC	2 cut(s)	181, 671
BspQI	GCTCTTC	1 cut(s)	508
BspT107I	GGYRCC	2 cut(s)	410, 666
BspTNI	GGTCTC	2 cut(s)	482, 562
BsrBI	CCGCTC	1 cut(s)	225
BsrDI	GCAATG	1 cut(s)	114
BsrI	ACTGG	1 cut(s)	607
BssECI	CCNNGG	1 cut(s)	530
BssMI	GATC	5 cut(s)	173, 359, 539, 676, 913
BssT1I	CCWWGG	1 cut(s)	530
Bst2UI	CCWGG	1 cut(s)	1019
Bst6I	CTCTTC	1 cut(s)	508
BstC8I	GCNNGC	1 cut(s)	949
BstF5I	GGATG	1 cut(s)	898
BstHHI	GCGC	1 cut(s)	271
BstKTI	GATC	5 cut(s)	176, 362, 542, 679, 916
BstMAI	GTCTC	3 cut(s)	482, 551, 562
BstMBI	GATC	5 cut(s)	173, 359, 539, 676, 913
BstMCI	CGRYCG	1 cut(s)	220
BstMWI	GCNNNNNNNGC	4 cut(s)	119, 316, 815, 944
BstNI	CCWGG	1 cut(s)	1019
BstNSI	RCATGY	2 cut(s)	776, 905
BstSCI	CCNGG	2 cut(s)	284, 1017
BstV1I	GCAGC	3 cut(s)	283, 286, 538
BstV2I	GAAGAC	1 cut(s)	174
BstX2I	RGATCY	3 cut(s)	173, 359, 539
BstYI	RGATCY	3 cut(s)	173, 359, 539
Bsu36I	CCTNAGG	1 cut(s)	888
BsuRI	GGCC	4 cut(s)	195, 215, 304, 763
BtsCI	GGATG	1 cut(s)	898
BtsI	GCAGTG	1 cut(s)	816
BtsIMutI	CAGTG	2 cut(s)	600, 816
BveI	ACCTGC	1 cut(s)	707
Cac8I	GCNNGC	1 cut(s)	949
CfoI	GCGC	1 cut(s)	271
Cfr13I	GGNCC	4 cut(s)	203, 213, 288, 1015
CseI	GACGC	1 cut(s)	27
Csp6I	GTAC	3 cut(s)	411, 481, 622
CviAII	CATG	3 cut(s)	313, 773, 902
CviQI	GTAC	3 cut(s)	411, 481, 622
DpnI	GATC	5 cut(s)	175, 361, 541, 678, 915
DpnII	GATC	5 cut(s)	173, 359, 539, 676, 913
DrdI	GACNNNNNNGTC	1 cut(s)	295
DseDI	GACNNNNNNGTC	1 cut(s)	295
Eam1104I	CTCTTC	1 cut(s)	508
EarI	CTCTTC	1 cut(s)	508
Eco130I	CCWWGG	1 cut(s)	530
Eco147I	AGGCCT	1 cut(s)	763
Eco31I	GGTCTC	2 cut(s)	482, 562
Eco47I	GGWCC	3 cut(s)	203, 288, 1015
Eco57I	CTGAAG	1 cut(s)	87
Eco81I	CCTNAGG	1 cut(s)	888
EcoO109I	RGGNCCY	1 cut(s)	203
EcoRII	CCWGG	1 cut(s)	1017
EcoT14I	CCWWGG	1 cut(s)	530
ErhI	CCWWGG	1 cut(s)	530
Esp3I	CGTCTC	1 cut(s)	551
FaeI	CATG	3 cut(s)	316, 776, 905
FaqI	GGGAC	2 cut(s)	216, 301
FatI	CATG	3 cut(s)	312, 772, 901
Fnu4HI	GCNGC	5 cut(s)	272, 275, 305, 308, 527
FokI	GGATG	1 cut(s)	905
Fsp4HI	GCNGC	5 cut(s)	272, 275, 305, 308, 527
FspBI	CTAG	3 cut(s)	300, 395, 939
GlaI	GCGC	1 cut(s)	270
GluI	GCNGC	5 cut(s)	272, 275, 305, 308, 527
GsaI	CCCAGC	1 cut(s)	433
HaeIII	GGCC	4 cut(s)	195, 215, 304, 763
HapII	CCGG	2 cut(s)	220, 285
HgaI	GACGC	1 cut(s)	27
HhaI	GCGC	1 cut(s)	271
Hin1II	CATG	3 cut(s)	316, 776, 905
Hin6I	GCGC	1 cut(s)	269
HinP1I	GCGC	1 cut(s)	269
HincII	GTYRAC	1 cut(s)	240
HindII	GTYRAC	1 cut(s)	240
HindIII	AAGCTT	2 cut(s)	99, 861
HinfI	GANTC	3 cut(s)	41, 281, 614
HpaII	CCGG	2 cut(s)	220, 285
HphI	GGTGA	2 cut(s)	383, 920
Hpy166II	GTNNAC	3 cut(s)	240, 788, 982
Hpy188I	TCNGA	7 cut(s)	35, 444, 487, 551, 613, 708, 1005
Hpy188III	TCNNGA	1 cut(s)	680
Hpy8I	GTNNAC	3 cut(s)	240, 788, 982
Hpy99I	CGWCG	1 cut(s)	250
HpyAV	CCTTC	4 cut(s)	435, 583, 685, 997
HpyCH4V	TGCA	3 cut(s)	803, 901, 947
HpyF10VI	GCNNNNNNNGC	4 cut(s)	119, 316, 815, 944
Hsp92II	CATG	3 cut(s)	316, 776, 905
HspAI	GCGC	1 cut(s)	269
KflI	GGGWCCC	1 cut(s)	203
KpnI	GGTACC	1 cut(s)	414
Kzo9I	GATC	5 cut(s)	173, 359, 539, 676, 913
LguI	GCTCTTC	1 cut(s)	508
LmnI	GCTCC	3 cut(s)	150, 222, 230
Lsp1109I	GCAGC	3 cut(s)	283, 286, 538
LweI	GCATC	6 cut(s)	391, 673, 790, 883, 934, 946
MaeI	CTAG	3 cut(s)	300, 395, 939
MalI	GATC	5 cut(s)	175, 361, 541, 678, 915
MbiI	CCGCTC	1 cut(s)	225
MboI	GATC	5 cut(s)	173, 359, 539, 676, 913
MboII	GAAGA	8 cut(s)	174, 359, 369, 466, 525, 549, 588, 641
MflI	RGATCY	3 cut(s)	173, 359, 539
MluCI	AATT	2 cut(s)	742, 869
MlyI	GAGTC	2 cut(s)	50, 290
MmeI	TCCRAC	1 cut(s)	869
MseI	TTAA	4 cut(s)	116, 723, 758, 858
MslI	CAYNNNNRTG	2 cut(s)	672, 906
MspI	CCGG	2 cut(s)	220, 285
MspR9I	CCNGG	2 cut(s)	286, 1019
MvaI	CCWGG	1 cut(s)	1019
MwoI	GCNNNNNNNGC	4 cut(s)	119, 316, 815, 944
NciI	CCSGG	1 cut(s)	286
NdeII	GATC	5 cut(s)	173, 359, 539, 676, 913
NlaIII	CATG	3 cut(s)	316, 776, 905
NlaIV	GGNNCC	8 cut(s)	146, 204, 205, 232, 289, 412, 668, 1016
NspI	RCATGY	2 cut(s)	776, 905
OliI	CACNNNNGTG	1 cut(s)	672
PaqCI	CACCTGC	1 cut(s)	707
PceI	AGGCCT	1 cut(s)	763
PciSI	GCTCTTC	1 cut(s)	508
PcsI	WCGNNNNNNNCGW	1 cut(s)	251
PfeI	GAWTC	1 cut(s)	614
PflMI	CCANNNNNTGG	1 cut(s)	436
PfoI	TCCNGGA	1 cut(s)	284
PkrI	GCNGC	5 cut(s)	273, 276, 306, 309, 528
PleI	GAGTC	2 cut(s)	49, 289
PpsI	GAGTC	2 cut(s)	49, 289
PpuMI	RGGWCCY	1 cut(s)	203
PsiI	TTATAA	1 cut(s)	639
Psp5II	RGGWCCY	1 cut(s)	203
Psp6I	CCWGG	1 cut(s)	1017
PspFI	CCCAGC	1 cut(s)	429
PspGI	CCWGG	1 cut(s)	1017
PspN4I	GGNNCC	8 cut(s)	146, 204, 205, 232, 289, 412, 668, 1016
PspPI	GGNCC	4 cut(s)	203, 213, 288, 1015
PspPPI	RGGWCCY	1 cut(s)	203
PsuI	RGATCY	3 cut(s)	173, 359, 539
RsaI	GTAC	3 cut(s)	412, 482, 623
RsaNI	GTAC	3 cut(s)	411, 481, 622
RseI	CAYNNNNRTG	2 cut(s)	672, 906
SapI	GCTCTTC	1 cut(s)	508
SaqAI	TTAA	4 cut(s)	116, 723, 758, 858
SatI	GCNGC	5 cut(s)	272, 275, 305, 308, 527
Sau3AI	GATC	5 cut(s)	173, 359, 539, 676, 913
Sau96I	GGNCC	4 cut(s)	203, 213, 288, 1015
ScaI	AGTACT	2 cut(s)	482, 623
SchI	GAGTC	2 cut(s)	50, 290
ScrFI	CCNGG	2 cut(s)	286, 1019
SfaNI	GCATC	6 cut(s)	391, 673, 790, 883, 934, 946
SinI	GGWCC	3 cut(s)	203, 288, 1015
SmiMI	CAYNNNNRTG	2 cut(s)	672, 906
SmlI	CTYRAG	1 cut(s)	197
SmoI	CTYRAG	1 cut(s)	197
Sse9I	AATT	2 cut(s)	742, 869
SseBI	AGGCCT	1 cut(s)	763
SsiI	CCGC	4 cut(s)	225, 263, 305, 308
SspI	AATATT	1 cut(s)	852
SspMI	CTAG	3 cut(s)	300, 395, 939
StuI	AGGCCT	1 cut(s)	763
StyD4I	CCNGG	2 cut(s)	284, 1017
StyI	CCWWGG	1 cut(s)	530
TaqI	TCGA	1 cut(s)	254
TaqII	GACCGA	1 cut(s)	506
TasI	AATT	2 cut(s)	742, 869
TatI	WGTACW	2 cut(s)	480, 621
TauI	GCSGC	2 cut(s)	307, 310
TfiI	GAWTC	1 cut(s)	614
Tru1I	TTAA	4 cut(s)	116, 723, 758, 858
Tru9I	TTAA	4 cut(s)	116, 723, 758, 858
TscAI	CASTG	2 cut(s)	607, 816
TseI	GCWGC	3 cut(s)	271, 274, 526
TspDTI	ATGAA	2 cut(s)	17, 111
TspGWI	ACGGA	1 cut(s)	486
TspRI	CASTG	2 cut(s)	607, 816
Van91I	CCANNNNNTGG	1 cut(s)	436
VpaK11BI	GGWCC	3 cut(s)	203, 288, 1015
XapI	RAATTY	1 cut(s)	742
XceI	RCATGY	2 cut(s)	776, 905
XspI	CTAG	3 cut(s)	300, 395, 939
ZrmI	AGTACT	2 cut(s)	482, 623

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.